Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0000660.1

Orthogroup ID

OG0006465

Physical Location & Seq

Genomic Coordinates Forward (+)

208730 .. 214703

Gene Structure 5974 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2514 bp

atgactggacttgaggttaagatagtggtgcttttggtggtcggtgttttggcgacggcgtcgtattgtgcaactgttagttacgaccacagggcgcttgtgattgatggaaagcgcagagtgttggtctccggctctattcactaccctcgtagcactccggagatgtggccggaccttattcagaaatccaaggacggagggctggatgtgatcgagacctatgtgttctggaactcacatgaacctgttcgaggcaagtacaattttgagggaaggaacgatttggttggattcgtgaaggcagtggcagcagcagggctctatgtgcatcttcgaattgggccttatgtgtgcgcagaatggaattatggtggctttcctctctggctgcattttatacccggaattaagttgcggactgataatgagccgtacaaggcagaaatgcagcggttcacggccaaaatagttgaaatgatgaagaatgagaagctttatgcatctcagggaggacccatcattttatcccagatcgagaacgagtatggaaacattgataaaccatatgggcctgccgccaaaacctacattaagtgggcagcaaacatggctgtgtctttggatacaggggtgccctgggtcatgtgccagcaagcagatgctccttcttcagtgatcaacacttgcaatggattttactgtgatcaattcaccccaaattctaacagcacacccaaaatttggaccgaaaattggagtggatggtttctttccttcggtggtgctgtacctcagagaccggtggaagaccttgcatttgctgtagcacgcttctaccagcgaggtggaactttccaaaattattatatgtaccatggtggaaccaactttgggcggtctagtggaggaccttttattgctacaagttatgactatgatgctccactcgatgagtatggacttcttaggcagccaaagtggggtcatctaaaagatgtgcataaggcgataaagctttgtgaagctgcaatggtggccactgacccaacaatttcttctcttggtcaaaacattgaggcagctgtttacaaaacaggatcagtatgttctgcttttcttgccaatatcgacaccaaatctgatgccactgtgactttcaatggaaattcatatcaattgcctgcatggtctgtgagcatcttacctgactgcaaaaatgtagtgcttaacaccgcaaagattaacactgccgctatgattccaagcttcacgcgtcaatcaatttcagctgatgttgagcccactggagccgtgggctcaggctggagttggataaatgaaccagtgggtatctcaaaggctgatgcatttactagaaacggattattggagcagataaacactacagctgataaaagtgactatttatggtactctacaagcattgatgtcaaagatggatataaagctgatcttcacgtggaatctcttggtcatgctcttcatgcttttgtcaatggaaagcttgcagggagtggaacaggcaacagtggcaatgccaaggtttctgtggacatccctgtcgaatttgcatctgggaagaacaaaattgatcttctgagtctgactgttgggcttcagaattatggatctttctttgacttggtgggtgctggaatcactggtcctgtacaactaaaaggctcaacaaatggcactactattgatctttcttctcaacaatggacatatcagattggacttaaaggtgaagatgaagatcttcccagtggaagttctcagtggatttcacaacctaccttgcccaagaatcagccattgacatggtacaagacccaatttgatgcccctggtggcagtaaccctgttgcactagacttcactggaatggggaagggtgaggcatgggtgaatggacaaagcattggacgttactggcccactaatattgctccaaaaagtggatgccctgacacttgcaattacagaggagcttatagtgcagacaaatgccgaaagaactgtggcaagccatctcaagaattgtaccacgttcctcgttcatggctgaaatccagtggcaatactcttgtattatttgaagaagttgggggtgatccgacacagttatcttttgccacgagacaggtagaaagtttgtgctctcatgtatcagagtctcacccatcacctgtagatatgtggagttcagattcaaaagcaggaaggaagtcacggccgaggctatcacttgagtgcccatttcctaatcaagtcatttcttcaatcaagtttgcaagttacggaagacctagtgggacttgtgggagctttagccatggctcgtgcaggagccgtagcgctctatccattgtccagaaggcgtgcattggatcaaaaagttgtagcatcgaagtctcgactaatacatttggtgacccatgtaaaggagtagcaaagagtttagcagtagaagtttcttgtaaatag

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0004565 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0015925 GO:0016787 GO:0016798 GO:0030312 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Gal_Lectin Glyco_hydro_35

Protein Analysis

837

Amino Acids

90.9

Weight (kDa)

7.73

Isoelectric Point (pI)

33.21

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_35	PF01301	33 - 337	3.3e-122	Glycosyl hydrolases family 35
Glyco_hydro_42	PF02449	54 - 202	2.3e-06	Beta-galactosidase
GHD	PF17834	361 - 415	3.1e-24	Beta-sandwich domain in beta galactosidase
BetaGal_gal-bd	PF21467	615 - 703	9.4e-26	Beta-galactosidase, galactose-binding domain
SUEL_Lectin	PF02140	759 - 836	8.6e-22	D-galactoside/L-rhamnose binding SUEL lectin domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006465)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28470 AT2G28470 AT2G28470 AT2G28470 AT2G28470
fragaria_vesca	FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630 FvH4_3g32630
malus_domestica	MD03G1114300.v1.1 MD11G1133400.v1.1
prunus_persica	Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1 Prupe.6G099700_v2.0.a1
pyrus_communis	pycom11g11010
rosa_chinensis	RchiOBHm_Chr5g0059701
rosa_laevigata	RLG00000035321
rosa_multiflora	Rmu_sc0000660.1_g000045
rosa_roxburghii	Rroxscaffold_1G00020930
rosa_rugosa	Rorug05G0324200
rosa_samantha	Rh5AG389700 Rh5BG401300 Rh5DG417700
rosa_wichuraiana	Rw5G036670

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1583
Acc16I	TGCGCA	1 cut(s)	358
AccB1I	GGYRCC	1 cut(s)	634
AccB7I	CCANNNNNTGG	2 cut(s)	744, 1985
AccII	CGCG	1 cut(s)	1276
AccIII	TCCGGA	1 cut(s)	160
AciI	CCGC	6 cut(s)	418, 454, 579, 898, 1236, 1254
AclWI	GGATC	4 cut(s)	1108, 1660, 2135, 2425
AcoI	YGGCCR	4 cut(s)	170, 462, 1038, 2261
AcsI	RAATTY	4 cut(s)	721, 741, 1168, 1589
AcuI	CTGAAG	2 cut(s)	657, 1625
AcvI	CACGTG	1 cut(s)	1483
AcyI	GRCGYC	1 cut(s)	59
AfaI	GTAC	8 cut(s)	263, 437, 792, 875, 1436, 1695, 1853, 2072
AfeI	AGCGCT	1 cut(s)	2386
AfiI	CCNNNNNNNGG	7 cut(s)	254, 744, 756, 894, 983, 1985, 2471
AflIII	ACRYGT	1 cut(s)	1274
AgeI	ACCGGT	1 cut(s)	802
AgsI	TTSAA	5 cut(s)	476, 1162, 2126, 2241, 2310
AjnI	CCWGG	2 cut(s)	638, 1873
AjuI	GAANNNNNNNTTGG	2 cut(s)	1042, 1074
AleI	CACNNNNGTG	1 cut(s)	2278
Alw21I	GWGCWC	1 cut(s)	2189
Alw26I	GTCTC	6 cut(s)	133, 212, 793, 2161, 2208, 2446
AlwI	GGATC	4 cut(s)	1108, 1660, 2135, 2425
Aor13HI	TCCGGA	1 cut(s)	160
Aor51HI	AGCGCT	1 cut(s)	2386
AoxI	GGCC	7 cut(s)	170, 344, 462, 572, 1038, 1961, 2261
ApeKI	GCWGC	8 cut(s)	311, 314, 391, 451, 602, 973, 1028, 1082
ApoI	RAATTY	4 cut(s)	721, 741, 1168, 1589
ArsI	GACNNNNNNTTYG	2 cut(s)	803, 835
AsiGI	ACCGGT	1 cut(s)	802
Asp700I	GAANNNNTTC	2 cut(s)	249, 1785
AspLEI	GCGC	4 cut(s)	97, 117, 359, 2387
AspS9I	GGNCC	8 cut(s)	175, 344, 515, 572, 747, 911, 1688, 1962
AsuC2I	CCSGG	1 cut(s)	405
AsuHPI	GGTGA	8 cut(s)	706, 1784, 1934, 1945, 2150, 2198, 2205, 2471
AsuII	TTCGAA	1 cut(s)	337
AvaII	GGWCC	5 cut(s)	175, 515, 747, 911, 1688
BaeGI	GKGCMC	2 cut(s)	639, 2285
BaeI	ACNNNNGTAYC	2 cut(s)	618, 651
BalI	TGGCCA	1 cut(s)	1040
BanI	GGYRCC	1 cut(s)	634
BanII	GRGCYC	3 cut(s)	324, 1305, 1322
BauI	CACGAG	2 cut(s)	2164, 2368
BbrPI	CACGTG	1 cut(s)	1483
BbsI	GAAGAC	2 cut(s)	816, 2338
Bbv12I	GWGCWC	1 cut(s)	2189
BbvI	GCAGC	8 cut(s)	323, 326, 378, 463, 614, 985, 1015, 1094
BccI	CCATC	6 cut(s)	101, 527, 759, 1454, 2065, 2218
BceAI	ACGGC	6 cut(s)	72, 418, 477, 1298, 2276, 2364
BciT130I	CCWGG	2 cut(s)	640, 1875
BciVI	GTATCC	1 cut(s)	619
BclI	TGATCA	2 cut(s)	678, 706
BcnI	CCSGG	1 cut(s)	405
BcoDI	GTCTC	6 cut(s)	133, 212, 793, 2161, 2208, 2446
BfaI	CTAG	4 cut(s)	903, 1377, 1898, 2337
BfmI	CTRYAG	3 cut(s)	825, 1407, 2217
BfoI	RGCGCY	2 cut(s)	98, 2388
BfuI	GTATCC	1 cut(s)	619
BglII	AGATCT	1 cut(s)	1783
Bme1390I	CCNGG	3 cut(s)	405, 640, 1875
Bme18I	GGWCC	5 cut(s)	175, 515, 747, 911, 1688
BmgT120I	GGNCC	8 cut(s)	175, 344, 515, 572, 747, 911, 1688, 1962
BmiI	GGNNCC	5 cut(s)	517, 636, 886, 1312, 2378
BmrFI	CCNGG	3 cut(s)	405, 640, 1875
BmrI	ACTGGG	1 cut(s)	1785
BmuI	ACTGGG	1 cut(s)	1785
BpiI	GAAGAC	2 cut(s)	816, 2338
BpmI	CTGGAG	2 cut(s)	1329, 1348
Bpu10I	CCTNAGC	1 cut(s)	1321
Bpu14I	TTCGAA	1 cut(s)	337
BpuEI	CTTGAG	3 cut(s)	32, 2046, 2297
BpuMI	CCSGG	1 cut(s)	405
BsaAI	YACGTR	1 cut(s)	1483
BsaBI	GATNNNNATC	1 cut(s)	1782
BsaHI	GRCGYC	1 cut(s)	59
BsaI	GGTCTC	3 cut(s)	133, 212, 793
BsaJI	CCNNGG	9 cut(s)	192, 638, 639, 877, 1314, 1563, 1873, 2264, 2362
BsaWI	WCCGGW	2 cut(s)	160, 802
Bsc4I	CCNNNNNNNGG	7 cut(s)	254, 744, 756, 894, 983, 1985, 2471
Bse118I	RCCGGY	1 cut(s)	802
Bse1I	ACTGG	8 cut(s)	10, 1312, 1346, 1690, 1791, 1912, 1964, 2100
Bse3DI	GCAATG	3 cut(s)	697, 1038, 1564
Bse8I	GATNNNNATC	1 cut(s)	1782
BseAI	TCCGGA	1 cut(s)	160
BseBI	CCWGG	2 cut(s)	640, 1875
BseDI	CCNNGG	9 cut(s)	192, 638, 639, 877, 1314, 1563, 1873, 2264, 2362
BseGI	GGATG	4 cut(s)	214, 770, 1578, 1994
BseJI	GATNNNNATC	1 cut(s)	1782
BseLI	CCNNNNNNNGG	7 cut(s)	254, 744, 756, 894, 983, 1985, 2471
BseMI	GCAATG	3 cut(s)	697, 1038, 1564
BseMII	CTCAG	5 cut(s)	521, 809, 1335, 1613, 1817
BseNI	ACTGG	8 cut(s)	10, 1312, 1346, 1690, 1791, 1912, 1964, 2100
BseRI	GAGGAG	1 cut(s)	2028
BseSI	GKGCMC	2 cut(s)	639, 2285
BseX3I	CGGCCG	1 cut(s)	2261
BseXI	GCAGC	8 cut(s)	323, 326, 378, 463, 614, 985, 1015, 1094
BsgI	GTGCAG	2 cut(s)	2046, 2392
Bsh1236I	CGCG	1 cut(s)	1276
Bsh1285I	CGRYCG	1 cut(s)	2264
BshFI	GGCC	7 cut(s)	172, 346, 464, 574, 1040, 1963, 2263
BshNI	GGYRCC	1 cut(s)	634
BshTI	ACCGGT	1 cut(s)	802
BsiEI	CGRYCG	1 cut(s)	2264
BsiHKAI	GWGCWC	1 cut(s)	2189
BsiSI	CCGG	5 cut(s)	132, 161, 173, 405, 803
BslFI	GGGAC	1 cut(s)	2356
BslI	CCNNNNNNNGG	7 cut(s)	254, 744, 756, 894, 983, 1985, 2471
BsmAI	GTCTC	6 cut(s)	133, 212, 793, 2161, 2208, 2446
BsmFI	GGGAC	1 cut(s)	2356
BsnI	GGCC	7 cut(s)	172, 346, 464, 574, 1040, 1963, 2263
Bso31I	GGTCTC	3 cut(s)	133, 212, 793
Bsp119I	TTCGAA	1 cut(s)	337
Bsp1286I	GDGCHC	6 cut(s)	324, 639, 1305, 1322, 2189, 2285
Bsp13I	TCCGGA	1 cut(s)	160
Bsp1407I	TGTACA	1 cut(s)	1693
Bsp19I	CCATGG	2 cut(s)	877, 2362
BspACI	CCGC	6 cut(s)	418, 454, 579, 898, 1236, 1254
BspANI	GGCC	7 cut(s)	172, 346, 464, 574, 1040, 1963, 2263
BspCNI	CTCAG	5 cut(s)	520, 808, 1334, 1614, 1816
BspEI	TCCGGA	1 cut(s)	160
BspFNI	CGCG	1 cut(s)	1276
BspLI	GGNNCC	5 cut(s)	517, 636, 886, 1312, 2378
BspPI	GGATC	4 cut(s)	1108, 1660, 2135, 2425
BspQI	GCTCTTC	1 cut(s)	1509
BspT104I	TTCGAA	1 cut(s)	337
BspT107I	GGYRCC	1 cut(s)	634
BspTNI	GGTCTC	3 cut(s)	133, 212, 793
BsrDI	GCAATG	3 cut(s)	697, 1038, 1564
BsrFI	RCCGGY	1 cut(s)	802
BsrGI	TGTACA	1 cut(s)	1693
BsrI	ACTGG	8 cut(s)	10, 1312, 1346, 1690, 1791, 1912, 1964, 2100
BssAI	RCCGGY	1 cut(s)	802
BssECI	CCNNGG	9 cut(s)	192, 638, 639, 877, 1314, 1563, 1873, 2264, 2362
BssNI	GRCGYC	1 cut(s)	59
BssSI	CACGAG	2 cut(s)	2164, 2368
BssT1I	CCWWGG	4 cut(s)	192, 877, 1563, 2362
Bst2BI	CACGAG	2 cut(s)	2164, 2368
Bst2UI	CCWGG	2 cut(s)	640, 1875
Bst4CI	ACNGT	7 cut(s)	76, 704, 1153, 1553, 1633, 2048, 2151
Bst6I	CTCTTC	1 cut(s)	1509
BstACI	GRCGYC	1 cut(s)	59
BstAUI	TGTACA	1 cut(s)	1693
BstBAI	YACGTR	1 cut(s)	1483
BstBI	TTCGAA	1 cut(s)	337
BstC8I	GCNNGC	8 cut(s)	576, 653, 657, 832, 1185, 1530, 2054, 2410
BstDEI	CTNAG	6 cut(s)	507, 795, 968, 1321, 1622, 1803
BstDSI	CCRYGG	3 cut(s)	877, 1314, 2362
BstEII	GGTNACC	1 cut(s)	2459
BstENI	CCTNNNNNAGG	1 cut(s)	252
BstF5I	GGATG	4 cut(s)	214, 770, 1578, 1994
BstFNI	CGCG	1 cut(s)	1276
BstH2I	RGCGCY	2 cut(s)	98, 2388
BstHHI	GCGC	4 cut(s)	97, 117, 359, 2387
BstMAI	GTCTC	6 cut(s)	133, 212, 793, 2161, 2208, 2446
BstMCI	CGRYCG	1 cut(s)	2264
BstMWI	GCNNNNNNNGC	8 cut(s)	311, 328, 611, 1037, 1121, 1508, 1554, 2024
BstNI	CCWGG	2 cut(s)	640, 1875
BstPI	GGTNACC	1 cut(s)	2459
BstSCI	CCNGG	3 cut(s)	403, 638, 1873
BstSFI	CTRYAG	3 cut(s)	825, 1407, 2217
BstSLI	GKGCMC	2 cut(s)	639, 2285
BstUI	CGCG	1 cut(s)	1276
BstV1I	GCAGC	8 cut(s)	323, 326, 378, 463, 614, 985, 1015, 1094
BstV2I	GAAGAC	2 cut(s)	816, 2338
BstX2I	RGATCY	2 cut(s)	1652, 1783
BstXI	CCANNNNNNTGG	2 cut(s)	848, 1848
BstYI	RGATCY	2 cut(s)	1652, 1783
BstZI	CGGCCG	1 cut(s)	2261
BsuI	GTATCC	1 cut(s)	619
BsuRI	GGCC	7 cut(s)	172, 346, 464, 574, 1040, 1963, 2263
BtgI	CCRYGG	3 cut(s)	877, 1314, 2362
BtsCI	GGATG	4 cut(s)	214, 770, 1578, 1994
BtsI	GCAGTG	2 cut(s)	312, 1248
Cac8I	GCNNGC	8 cut(s)	576, 653, 657, 832, 1185, 1530, 2054, 2410
CfoI	GCGC	4 cut(s)	97, 117, 359, 2387
Cfr10I	RCCGGY	1 cut(s)	802
Cfr13I	GGNCC	8 cut(s)	175, 344, 515, 572, 747, 911, 1688, 1962
CseI	GACGC	2 cut(s)	48, 1265
Csp6I	GTAC	8 cut(s)	262, 436, 791, 874, 1435, 1694, 1852, 2071
CspAI	ACCGGT	1 cut(s)	802
CspCI	CAANNNNNGTGG	2 cut(s)	1954, 1989
CviQI	GTAC	8 cut(s)	262, 436, 791, 874, 1435, 1694, 1852, 2071
DdeI	CTNAG	6 cut(s)	507, 795, 968, 1321, 1622, 1803
DrdI	GACNNNNNNGTC	1 cut(s)	1583
DseDI	GACNNNNNNGTC	1 cut(s)	1583
EaeI	YGGCCR	4 cut(s)	170, 462, 1038, 2261
EagI	CGGCCG	1 cut(s)	2261
Eam1104I	CTCTTC	1 cut(s)	1509
EarI	CTCTTC	1 cut(s)	1509
EclXI	CGGCCG	1 cut(s)	2261
Eco130I	CCWWGG	4 cut(s)	192, 877, 1563, 2362
Eco24I	GRGCYC	3 cut(s)	324, 1305, 1322
Eco31I	GGTCTC	3 cut(s)	133, 212, 793
Eco47I	GGWCC	5 cut(s)	175, 515, 747, 911, 1688
Eco47III	AGCGCT	1 cut(s)	2386
Eco52I	CGGCCG	1 cut(s)	2261
Eco57I	CTGAAG	2 cut(s)	657, 1625
Eco72I	CACGTG	1 cut(s)	1483
Eco91I	GGTNACC	1 cut(s)	2459
EcoNI	CCTNNNNNAGG	1 cut(s)	252
EcoO109I	RGGNCCY	2 cut(s)	515, 911
EcoO65I	GGTNACC	1 cut(s)	2459
EcoRII	CCWGG	2 cut(s)	638, 1873
EcoT14I	CCWWGG	4 cut(s)	192, 877, 1563, 2362
EcoT22I	ATGCAT	2 cut(s)	505, 1372
EcoT38I	GRGCYC	3 cut(s)	324, 1305, 1322
ErhI	CCWWGG	4 cut(s)	192, 877, 1563, 2362
FalI	AAGNNNNNCTT	2 cut(s)	1461, 1493
FaqI	GGGAC	1 cut(s)	2356
FauNDI	CATATG	1 cut(s)	568
FbaI	TGATCA	2 cut(s)	678, 706
FokI	GGATG	4 cut(s)	221, 777, 1565, 2001
FriOI	GRGCYC	3 cut(s)	324, 1305, 1322
FspBI	CTAG	4 cut(s)	903, 1377, 1898, 2337
FspI	TGCGCA	1 cut(s)	358
GlaI	GCGC	4 cut(s)	96, 116, 358, 2386
GsuI	CTGGAG	2 cut(s)	1329, 1348
HaeII	RGCGCY	2 cut(s)	98, 2388
HaeIII	GGCC	7 cut(s)	172, 346, 464, 574, 1040, 1963, 2263
HapII	CCGG	5 cut(s)	132, 161, 173, 405, 803
HgaI	GACGC	2 cut(s)	48, 1265
HhaI	GCGC	4 cut(s)	97, 117, 359, 2387
Hin1I	GRCGYC	1 cut(s)	59
Hin6I	GCGC	4 cut(s)	95, 115, 357, 2385
HinP1I	GCGC	4 cut(s)	95, 115, 357, 2385
HindIII	AAGCTT	4 cut(s)	494, 1016, 1267, 1526
HinfI	GANTC	8 cut(s)	294, 1261, 1487, 1624, 1680, 1834, 2201, 2237
HpaII	CCGG	5 cut(s)	132, 161, 173, 405, 803
HphI	GGTGA	8 cut(s)	706, 1784, 1934, 1945, 2150, 2198, 2205, 2471
Hpy166II	GTNNAC	3 cut(s)	459, 1090, 1576
Hpy188III	TCNNGA	8 cut(s)	161, 217, 232, 298, 538, 2063, 2401, 2443
Hpy8I	GTNNAC	3 cut(s)	459, 1090, 1576
Hpy99I	CGWCG	2 cut(s)	58, 64
HpyAV	CCTTC	7 cut(s)	270, 295, 678, 787, 1912, 2244, 2398
HpyCH4III	ACNGT	7 cut(s)	76, 704, 1153, 1553, 1633, 2048, 2151
HpyCH4IV	ACGT	3 cut(s)	1482, 1954, 2076
HpyF10VI	GCNNNNNNNGC	8 cut(s)	311, 328, 611, 1037, 1121, 1508, 1554, 2024
HpyF3I	CTNAG	6 cut(s)	507, 795, 968, 1321, 1622, 1803
HpySE526I	ACGT	3 cut(s)	1482, 1954, 2076
Hsp92I	GRCGYC	1 cut(s)	59
HspAI	GCGC	4 cut(s)	95, 115, 357, 2385
Kpn2I	TCCGGA	1 cut(s)	160
Ksp22I	TGATCA	2 cut(s)	678, 706
LguI	GCTCTTC	1 cut(s)	1509
LmnI	GCTCC	8 cut(s)	670, 949, 1310, 1393, 1981, 2015, 2352, 2376
Lsp1109I	GCAGC	8 cut(s)	323, 326, 378, 463, 614, 985, 1015, 1094
MaeI	CTAG	4 cut(s)	903, 1377, 1898, 2337
MaeII	ACGT	3 cut(s)	1482, 1954, 2076
MaeIII	GTNAC	8 cut(s)	80, 1153, 1421, 1883, 1955, 2256, 2324, 2459
MfeI	CAATTG	1 cut(s)	1178
MflI	RGATCY	2 cut(s)	1652, 1783
MhlI	GDGCHC	6 cut(s)	324, 639, 1305, 1322, 2189, 2285
MlsI	TGGCCA	1 cut(s)	1040
MluI	ACGCGT	1 cut(s)	1274
MluNI	TGGCCA	1 cut(s)	1040
MlyI	GAGTC	2 cut(s)	1633, 2210
MmeI	TCCRAC	3 cut(s)	271, 1313, 2168
Mox20I	TGGCCA	1 cut(s)	1040
Mph1103I	ATGCAT	2 cut(s)	505, 1372
MroI	TCCGGA	1 cut(s)	160
MroXI	GAANNNNTTC	2 cut(s)	249, 1785
MscI	TGGCCA	1 cut(s)	1040
MseI	TTAA	6 cut(s)	18, 411, 594, 1230, 1245, 1767
MslI	CAYNNNNRTG	4 cut(s)	614, 1846, 1910, 2278
Msp20I	TGGCCA	1 cut(s)	1040
MspA1I	CMGCKG	4 cut(s)	454, 1085, 1292, 1412
MspI	CCGG	5 cut(s)	132, 161, 173, 405, 803
MspR9I	CCNGG	3 cut(s)	405, 640, 1875
MunI	CAATTG	1 cut(s)	1178
MvaI	CCWGG	2 cut(s)	640, 1875
MvnI	CGCG	1 cut(s)	1276
MwoI	GCNNNNNNNGC	8 cut(s)	311, 328, 611, 1037, 1121, 1508, 1554, 2024
NciI	CCSGG	1 cut(s)	405
NcoI	CCATGG	2 cut(s)	877, 2362
NdeI	CATATG	1 cut(s)	568
NlaIV	GGNNCC	5 cut(s)	517, 636, 886, 1312, 2378
NmeAIII	GCCGAG	1 cut(s)	2289
NmuCI	GTSAC	4 cut(s)	1153, 1421, 2256, 2459
NsbI	TGCGCA	1 cut(s)	358
NsiI	ATGCAT	2 cut(s)	505, 1372
NspV	TTCGAA	1 cut(s)	337
OliI	CACNNNNGTG	1 cut(s)	2278
PasI	CCCWGGG	1 cut(s)	639
PciSI	GCTCTTC	1 cut(s)	1509
PdmI	GAANNNNTTC	2 cut(s)	249, 1785
PfeI	GAWTC	6 cut(s)	294, 1261, 1487, 1680, 1834, 2237
PflFI	GACNNNGTC	1 cut(s)	58
PflMI	CCANNNNNTGG	2 cut(s)	744, 1985
PinAI	ACCGGT	1 cut(s)	802
PleI	GAGTC	2 cut(s)	1632, 2209
PmaCI	CACGTG	1 cut(s)	1483
PmlI	CACGTG	1 cut(s)	1483
PpsI	GAGTC	2 cut(s)	1632, 2209
Ppu21I	YACGTR	1 cut(s)	1483
PpuMI	RGGWCCY	2 cut(s)	515, 911
Psp5II	RGGWCCY	2 cut(s)	515, 911
Psp6I	CCWGG	2 cut(s)	638, 1873
PspCI	CACGTG	1 cut(s)	1483
PspEI	GGTNACC	1 cut(s)	2459
PspGI	CCWGG	2 cut(s)	638, 1873
PspN4I	GGNNCC	5 cut(s)	517, 636, 886, 1312, 2378
PspPI	GGNCC	8 cut(s)	175, 344, 515, 572, 747, 911, 1688, 1962
PspPPI	RGGWCCY	2 cut(s)	515, 911
PsuI	RGATCY	2 cut(s)	1652, 1783
PsyI	GACNNNGTC	1 cut(s)	58
PvuII	CAGCTG	3 cut(s)	1085, 1292, 1412
RsaI	GTAC	8 cut(s)	263, 437, 792, 875, 1436, 1695, 1853, 2072
RsaNI	GTAC	8 cut(s)	262, 436, 791, 874, 1435, 1694, 1852, 2071
RseI	CAYNNNNRTG	4 cut(s)	614, 1846, 1910, 2278
SapI	GCTCTTC	1 cut(s)	1509
SaqAI	TTAA	6 cut(s)	18, 411, 594, 1230, 1245, 1767
Sau96I	GGNCC	8 cut(s)	175, 344, 515, 572, 747, 911, 1688, 1962
SchI	GAGTC	2 cut(s)	1633, 2210
ScrFI	CCNGG	3 cut(s)	405, 640, 1875
SduI	GDGCHC	6 cut(s)	324, 639, 1305, 1322, 2189, 2285
SfcI	CTRYAG	3 cut(s)	825, 1407, 2217
SfuI	TTCGAA	1 cut(s)	337
SinI	GGWCC	5 cut(s)	175, 515, 747, 911, 1688
SmiMI	CAYNNNNRTG	4 cut(s)	614, 1846, 1910, 2278
SmlI	CTYRAG	3 cut(s)	11, 2061, 2276
SmoI	CTYRAG	3 cut(s)	11, 2061, 2276
SsiI	CCGC	6 cut(s)	418, 454, 579, 898, 1236, 1254
SspI	AATATT	1 cut(s)	1972
SspMI	CTAG	4 cut(s)	903, 1377, 1898, 2337
StyD4I	CCNGG	3 cut(s)	403, 638, 1873
StyI	CCWWGG	4 cut(s)	192, 877, 1563, 2362
TaaI	ACNGT	7 cut(s)	76, 704, 1153, 1553, 1633, 2048, 2151
TaiI	ACGT	3 cut(s)	1485, 1957, 2079
TaqI	TCGA	9 cut(s)	216, 253, 337, 537, 951, 1131, 1587, 2436, 2444
TaqII	GACCGA	1 cut(s)	764
TatI	WGTACW	2 cut(s)	261, 1693
TauI	GCSGC	2 cut(s)	581, 1256
TfiI	GAWTC	6 cut(s)	294, 1261, 1487, 1680, 1834, 2237
Tru1I	TTAA	6 cut(s)	18, 411, 594, 1230, 1245, 1767
Tru9I	TTAA	6 cut(s)	18, 411, 594, 1230, 1245, 1767
TseFI	GTSAC	4 cut(s)	1153, 1421, 2256, 2459
TseI	GCWGC	8 cut(s)	311, 314, 391, 451, 602, 973, 1028, 1082
Tsp45I	GTSAC	4 cut(s)	1153, 1421, 2256, 2459
TspDTI	ATGAA	7 cut(s)	258, 497, 1161, 1356, 1496, 1794, 2076
TspGWI	ACGGA	3 cut(s)	213, 1398, 2343
Tth111I	GACNNNGTC	1 cut(s)	58
Van91I	CCANNNNNTGG	2 cut(s)	744, 1985
VpaK11BI	GGWCC	5 cut(s)	175, 515, 747, 911, 1688
XagI	CCTNNNNNAGG	1 cut(s)	252
XapI	RAATTY	4 cut(s)	721, 741, 1168, 1589
XcmI	CCANNNNNNNNNTGG	2 cut(s)	1312, 1570
XmnI	GAANNNNTTC	2 cut(s)	249, 1785
XspI	CTAG	4 cut(s)	903, 1377, 1898, 2337
Zsp2I	ATGCAT	2 cut(s)	505, 1372

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.