Rmu_sc0000692.1_g000010

Ribonuclease H

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000692.1
OG0012710
Physical Location & Seq
Reverse (-)
43645 .. 50708
7064 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1245 bp
atggtttgtcgtcctaaggagtatggtgtcttgggaatcaaaagtacttcgcaaatgaatcaagctcttcttgcaaaacttgggtggagattaactcaaggagataatggattgtggagaaagcttaatcagggattgtcatggaggattggtgatggtaatattgtgaatttttggactgacatttggactccaggtggacctttgattaaggatgctgtaagctctagaaatgttgatttgaatctgaaggtgaaggatttttggaacggtgttggttggaacctagatttgataagacaatgtgtgagtgaggattccattagcgaaattatccaagtccctgctggtttccttggtggtggacatgataaattgacctggcgggctacatcgaatggtaatttcactgatctgaagggaagcaaacccgaccattctgcagtccaggcgtggccagaaactggagctgtgatcaaagaggggaaagagatggccgaagagaagaaaggaggaagagatcagcgcctcaatgataatgttggtagtgcatactatgttgcacctgaagtgctacatagatcctacagtgttgaagcagatatgtggagcataggggctctttccaaggctatgacagaagatgatctctactatcttagggctcagtttacgctcttggaacccaaacatggatacgtgtccctcgacaatttcagaacgattaagacccgcaaactgaatattgcagctccactggaccctgcagcttttgccgatgcagtagttccagattttatctggataatgctggtgatatgtgacatatcatatgacaacgggctcactccccagatcatcctattcagtcaattggatttaactatgctagagagtttacatccaaagactttcgatggatattgcaatgggcatgatgttggacatcaaattcacttgtacacacccaaatggtcttgcggaaatgactacgatgatagtccagacattggtaatgcgcaccaatctccttatatccgcttgggatttgcgggttgtcactttgatgagacagtcttcctgtcgttagggggagataagaacacggatcttcagcaggaacgacaggaattgtcgtggcctgtccccactatgtctcatctcgatccctattacagtgacgagatcacacaaatatgctgcaaacaaacctgcaaggaaggacgtctctacgagaggacgtag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_3 zf-RVT

Protein Analysis

414

Amino Acids

46.49

Weight (kDa)

5.32

Isoelectric Point (pI)

29.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 173 - 218 7e-07 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012710)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G46700 AT2G46700 AT2G46700
fragaria_vesca FvH4_7g17350 FvH4_7g17350
malus_domestica MD01G1086500.v1.1 MD07G1156500.v1.1
prunus_persica Prupe.2G194900_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0360791
rosa_laevigata RLG00000027786
rosa_multiflora Rmu_sc0000692.1_g000010
rosa_roxburghii Rroxscaffold_4G00295810
rosa_rugosa Rorug01G0281500
rosa_samantha Rh1AG293500 Rh1BG258100 Rh1CG276000 Rh1DG288400
rosa_wichuraiana Rw1G025970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1228
Acc16I TGCGCA 1 cut(s) 1020
Acc36I ACCTGC 1 cut(s) 1220
AccB7I CCANNNNNTGG 2 cut(s) 464, 1010
AciI CCGC 5 cut(s) 385, 733, 981, 1039, 1052
AclWI GGATC 3 cut(s) 576, 1116, 1160
AcoI YGGCCR 2 cut(s) 455, 495
AcsI RAATTY 2 cut(s) 169, 951
AcuI CTGAAG 4 cut(s) 269, 437, 588, 1097
AcyI GRCGYC 1 cut(s) 1225
AfaI GTAC 2 cut(s) 46, 962
AfiI CCNNNNNNNGG 3 cut(s) 464, 692, 1010
AflIII ACRYGT 1 cut(s) 699
AgsI TTSAA 2 cut(s) 244, 596
AjnI CCWGG 3 cut(s) 193, 380, 447
AluBI AGCT 6 cut(s) 65, 124, 225, 470, 752, 770
AluI AGCT 6 cut(s) 65, 124, 225, 470, 752, 770
Alw26I GTCTC 3 cut(s) 1064, 1161, 1232
AlwI GGATC 3 cut(s) 576, 1116, 1160
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 3 cut(s) 455, 495, 1139
ApeKI GCWGC 3 cut(s) 749, 767, 1200
ApoI RAATTY 2 cut(s) 169, 951
AspLEI GCGC 2 cut(s) 528, 1021
AspS9I GGNCC 2 cut(s) 200, 760
AsuHPI GGTGA 3 cut(s) 164, 265, 826
AvaII GGWCC 2 cut(s) 200, 760
AxyI CCTNAGG 1 cut(s) 15
BalI TGGCCA 1 cut(s) 457
BanII GRGCYC 3 cut(s) 622, 667, 846
BbsI GAAGAC 1 cut(s) 1069
BbvI GCAGC 3 cut(s) 761, 779, 1187
BccI CCATC 3 cut(s) 149, 487, 911
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciT130I CCWGG 3 cut(s) 195, 382, 449
BciVI GTATCC 1 cut(s) 689
BclI TGATCA 1 cut(s) 474
BcoDI GTCTC 3 cut(s) 1064, 1161, 1232
BfaI CTAG 3 cut(s) 228, 287, 890
BfmI CTRYAG 3 cut(s) 441, 586, 765
BfoI RGCGCY 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 1220
BfuI GTATCC 1 cut(s) 689
BisI GCNGC 3 cut(s) 750, 768, 1201
BlsI GCNGC 3 cut(s) 751, 769, 1202
BmcAI AGTACT 1 cut(s) 46
Bme1390I CCNGG 3 cut(s) 195, 382, 449
Bme18I GGWCC 2 cut(s) 200, 760
BmgT120I GGNCC 2 cut(s) 200, 760
BmiI GGNNCC 3 cut(s) 284, 684, 762
BmrFI CCNGG 3 cut(s) 195, 382, 449
BmsI GCATC 2 cut(s) 205, 769
BpiI GAAGAC 1 cut(s) 1069
BplI GAGNNNNNCTC 2 cut(s) 79, 111
BpmI CTGGAG 2 cut(s) 177, 486
BpuEI CTTGAG 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 700
BsaBI GATNNNNATC 1 cut(s) 243
BsaHI GRCGYC 1 cut(s) 1225
BsaJI CCNNGG 2 cut(s) 355, 627
BsaXI ACNNNNNCTCC 2 cut(s) 136, 166
Bsc4I CCNNNNNNNGG 3 cut(s) 464, 692, 1010
Bse1I ACTGG 2 cut(s) 469, 762
Bse21I CCTNAGG 1 cut(s) 15
Bse3DI GCAATG 1 cut(s) 934
Bse8I GATNNNNATC 1 cut(s) 243
BseBI CCWGG 3 cut(s) 195, 382, 449
BseDI CCNNGG 2 cut(s) 355, 627
BseGI GGATG 3 cut(s) 220, 858, 901
BseJI GATNNNNATC 1 cut(s) 243
BseLI CCNNNNNNNGG 3 cut(s) 464, 692, 1010
BseMI GCAATG 1 cut(s) 934
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 2 cut(s) 469, 762
BseXI GCAGC 3 cut(s) 761, 779, 1187
BshFI GGCC 3 cut(s) 457, 497, 1141
BslFI GGGAC 3 cut(s) 326, 688, 1130
BslI CCNNNNNNNGG 3 cut(s) 464, 692, 1010
BsmAI GTCTC 3 cut(s) 1064, 1161, 1232
BsmBI CGTCTC 1 cut(s) 1232
BsmFI GGGAC 3 cut(s) 326, 688, 1130
BsnI GGCC 3 cut(s) 457, 497, 1141
Bsp1286I GDGCHC 3 cut(s) 622, 667, 846
Bsp1407I TGTACA 1 cut(s) 960
Bsp143I GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
BspACI CCGC 5 cut(s) 385, 733, 981, 1039, 1052
BspANI GGCC 3 cut(s) 457, 497, 1141
BspCNI CTCAG 1 cut(s) 679
BspLI GGNNCC 3 cut(s) 284, 684, 762
BspMAI CTGCAG 2 cut(s) 445, 769
BspMI ACCTGC 1 cut(s) 1220
BspPI GGATC 3 cut(s) 576, 1116, 1160
BspQI GCTCTTC 1 cut(s) 72
BsrDI GCAATG 1 cut(s) 934
BsrGI TGTACA 1 cut(s) 960
BsrI ACTGG 2 cut(s) 469, 762
BssECI CCNNGG 2 cut(s) 355, 627
BssMI GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
BssNI GRCGYC 1 cut(s) 1225
BssT1I CCWWGG 2 cut(s) 355, 627
Bst2UI CCWGG 3 cut(s) 195, 382, 449
Bst4CI ACNGT 4 cut(s) 272, 590, 1075, 1178
Bst6I CTCTTC 3 cut(s) 72, 495, 511
BstACI GRCGYC 1 cut(s) 1225
BstAPI GCANNNNNTGC 1 cut(s) 773
BstAUI TGTACA 1 cut(s) 960
BstBAI YACGTR 1 cut(s) 700
BstC8I GCNNGC 1 cut(s) 387
BstDEI CTNAG 3 cut(s) 15, 659, 666
BstF5I GGATG 3 cut(s) 220, 858, 901
BstH2I RGCGCY 1 cut(s) 529
BstHHI GCGC 2 cut(s) 528, 1021
BstKTI GATC 9 cut(s) 415, 477, 523, 584, 649, 858, 1111, 1168, 1188
BstMAI GTCTC 3 cut(s) 1064, 1161, 1232
BstMBI GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
BstMWI GCNNNNNNNGC 3 cut(s) 71, 449, 773
BstNI CCWGG 3 cut(s) 195, 382, 449
BstSCI CCNGG 3 cut(s) 193, 380, 447
BstSFI CTRYAG 3 cut(s) 441, 586, 765
BstV1I GCAGC 3 cut(s) 761, 779, 1187
BstV2I GAAGAC 1 cut(s) 1069
BstX2I RGATCY 2 cut(s) 581, 1108
BstYI RGATCY 2 cut(s) 581, 1108
Bsu36I CCTNAGG 1 cut(s) 15
BsuI GTATCC 1 cut(s) 689
BsuRI GGCC 3 cut(s) 457, 497, 1141
BtsCI GGATG 3 cut(s) 220, 858, 901
BtsIMutI CAGTG 4 cut(s) 408, 595, 755, 1183
BveI ACCTGC 1 cut(s) 1220
Cac8I GCNNGC 1 cut(s) 387
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 2 cut(s) 528, 1021
Cfr13I GGNCC 2 cut(s) 200, 760
Csp6I GTAC 2 cut(s) 45, 961
CviAII CATG 4 cut(s) 141, 368, 692, 935
CviQI GTAC 2 cut(s) 45, 961
DdeI CTNAG 3 cut(s) 15, 659, 666
DpnI GATC 9 cut(s) 414, 476, 522, 583, 648, 857, 1110, 1167, 1187
DpnII GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
EaeI YGGCCR 2 cut(s) 455, 495
Eam1104I CTCTTC 3 cut(s) 72, 495, 511
EarI CTCTTC 3 cut(s) 72, 495, 511
Eco130I CCWWGG 2 cut(s) 355, 627
Eco24I GRGCYC 3 cut(s) 622, 667, 846
Eco47I GGWCC 2 cut(s) 200, 760
Eco57I CTGAAG 4 cut(s) 269, 437, 588, 1097
Eco81I CCTNAGG 1 cut(s) 15
EcoRII CCWGG 3 cut(s) 193, 380, 447
EcoT14I CCWWGG 2 cut(s) 355, 627
EcoT38I GRGCYC 3 cut(s) 622, 667, 846
ErhI CCWWGG 2 cut(s) 355, 627
Esp3I CGTCTC 1 cut(s) 1232
FaeI CATG 4 cut(s) 144, 371, 695, 938
FalI AAGNNNNNCTT 2 cut(s) 54, 86
FaqI GGGAC 3 cut(s) 326, 688, 1130
FatI CATG 4 cut(s) 140, 367, 691, 934
FauI CCCGC 3 cut(s) 378, 740, 1045
FauNDI CATATG 1 cut(s) 832
FbaI TGATCA 1 cut(s) 474
Fnu4HI GCNGC 3 cut(s) 750, 768, 1201
FokI GGATG 3 cut(s) 227, 845, 888
FriOI GRGCYC 3 cut(s) 622, 667, 846
Fsp4HI GCNGC 3 cut(s) 750, 768, 1201
FspAI RTGCGCAY 1 cut(s) 1020
FspBI CTAG 3 cut(s) 228, 287, 890
FspI TGCGCA 1 cut(s) 1020
GlaI GCGC 2 cut(s) 527, 1020
GluI GCNGC 3 cut(s) 750, 768, 1201
GsuI CTGGAG 2 cut(s) 177, 486
HaeII RGCGCY 1 cut(s) 529
HaeIII GGCC 3 cut(s) 457, 497, 1141
HhaI GCGC 2 cut(s) 528, 1021
Hin1I GRCGYC 1 cut(s) 1225
Hin1II CATG 4 cut(s) 144, 371, 695, 938
Hin6I GCGC 2 cut(s) 526, 1019
HinP1I GCGC 2 cut(s) 526, 1019
HindIII AAGCTT 1 cut(s) 122
HinfI GANTC 5 cut(s) 36, 58, 190, 244, 317
HphI GGTGA 3 cut(s) 164, 265, 826
Hpy166II GTNNAC 5 cut(s) 200, 365, 672, 899, 963
Hpy188I TCNGA 3 cut(s) 249, 417, 719
Hpy188III TCNNGA 5 cut(s) 228, 791, 802, 1004, 1163
Hpy8I GTNNAC 5 cut(s) 200, 365, 672, 899, 963
HpyAV CCTTC 4 cut(s) 244, 250, 412, 1214
HpyCH4III ACNGT 4 cut(s) 272, 590, 1075, 1178
HpyCH4IV ACGT 3 cut(s) 699, 1225, 1241
HpyF10VI GCNNNNNNNGC 3 cut(s) 71, 449, 773
HpyF3I CTNAG 3 cut(s) 15, 659, 666
HpySE526I ACGT 3 cut(s) 699, 1225, 1241
Hsp92I GRCGYC 1 cut(s) 1225
Hsp92II CATG 4 cut(s) 144, 371, 695, 938
HspAI GCGC 2 cut(s) 526, 1019
Ksp22I TGATCA 1 cut(s) 474
Kzo9I GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
LguI GCTCTTC 1 cut(s) 72
LmnI GCTCC 3 cut(s) 467, 609, 757
Lsp1109I GCAGC 3 cut(s) 761, 779, 1187
LweI GCATC 2 cut(s) 205, 769
MaeI CTAG 3 cut(s) 228, 287, 890
MaeII ACGT 3 cut(s) 699, 1225, 1241
MaeIII GTNAC 3 cut(s) 821, 1058, 1178
MalI GATC 9 cut(s) 414, 476, 522, 583, 648, 857, 1110, 1167, 1187
MboI GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
MboII GAAGA 7 cut(s) 59, 512, 517, 528, 653, 1069, 1103
MfeI CAATTG 1 cut(s) 872
MflI RGATCY 2 cut(s) 581, 1108
MhlI GDGCHC 3 cut(s) 622, 667, 846
MlsI TGGCCA 1 cut(s) 457
MluCI AATT 8 cut(s) 169, 330, 374, 403, 712, 872, 951, 1130
MluNI TGGCCA 1 cut(s) 457
MlyI GAGTC 1 cut(s) 184
MmeI TCCRAC 2 cut(s) 260, 922
MnlI CCTC 7 cut(s) 138, 307, 475, 506, 539, 716, 1230
Mox20I TGGCCA 1 cut(s) 457
MscI TGGCCA 1 cut(s) 457
MseI TTAA 5 cut(s) 92, 126, 210, 726, 881
MslI CAYNNNNRTG 3 cut(s) 970, 1065, 1195
Msp20I TGGCCA 1 cut(s) 457
MspR9I CCNGG 3 cut(s) 195, 382, 449
MunI CAATTG 1 cut(s) 872
MvaI CCWGG 3 cut(s) 195, 382, 449
MwoI GCNNNNNNNGC 3 cut(s) 71, 449, 773
NdeI CATATG 1 cut(s) 832
NdeII GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
NlaIII CATG 4 cut(s) 144, 371, 695, 938
NlaIV GGNNCC 3 cut(s) 284, 684, 762
NmuCI GTSAC 3 cut(s) 821, 1058, 1178
NsbI TGCGCA 1 cut(s) 1020
PciSI GCTCTTC 1 cut(s) 72
PcsI WCGNNNNNNNCGW 1 cut(s) 705
PfeI GAWTC 4 cut(s) 36, 58, 244, 317
PflMI CCANNNNNTGG 2 cut(s) 464, 1010
PkrI GCNGC 3 cut(s) 751, 769, 1202
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
Ppu21I YACGTR 1 cut(s) 700
Psp6I CCWGG 3 cut(s) 193, 380, 447
PspGI CCWGG 3 cut(s) 193, 380, 447
PspN4I GGNNCC 3 cut(s) 284, 684, 762
PspPI GGNCC 2 cut(s) 200, 760
PstI CTGCAG 2 cut(s) 445, 769
PstNI CAGNNNCTG 1 cut(s) 464
PsuI RGATCY 2 cut(s) 581, 1108
RsaI GTAC 2 cut(s) 46, 962
RsaNI GTAC 2 cut(s) 45, 961
RseI CAYNNNNRTG 3 cut(s) 970, 1065, 1195
SapI GCTCTTC 1 cut(s) 72
SaqAI TTAA 5 cut(s) 92, 126, 210, 726, 881
SatI GCNGC 3 cut(s) 750, 768, 1201
Sau3AI GATC 9 cut(s) 412, 474, 520, 581, 646, 855, 1108, 1165, 1185
Sau96I GGNCC 2 cut(s) 200, 760
ScaI AGTACT 1 cut(s) 46
SchI GAGTC 1 cut(s) 184
ScrFI CCNGG 3 cut(s) 195, 382, 449
SduI GDGCHC 3 cut(s) 622, 667, 846
SfaNI GCATC 2 cut(s) 205, 769
SfcI CTRYAG 3 cut(s) 441, 586, 765
SinI GGWCC 2 cut(s) 200, 760
SmiMI CAYNNNNRTG 3 cut(s) 970, 1065, 1195
SmlI CTYRAG 1 cut(s) 96
SmoI CTYRAG 1 cut(s) 96
Sse9I AATT 8 cut(s) 169, 330, 374, 403, 712, 872, 951, 1130
SsiI CCGC 5 cut(s) 385, 733, 981, 1039, 1052
SspI AATATT 2 cut(s) 163, 745
SspMI CTAG 3 cut(s) 228, 287, 890
StyD4I CCNGG 3 cut(s) 193, 380, 447
StyI CCWWGG 2 cut(s) 355, 627
TaaI ACNGT 4 cut(s) 272, 590, 1075, 1178
TaiI ACGT 3 cut(s) 702, 1228, 1244
TaqI TCGA 4 cut(s) 395, 708, 915, 1164
TasI AATT 8 cut(s) 169, 330, 374, 403, 712, 872, 951, 1130
TatI WGTACW 2 cut(s) 44, 960
TfiI GAWTC 4 cut(s) 36, 58, 244, 317
Tru1I TTAA 5 cut(s) 92, 126, 210, 726, 881
Tru9I TTAA 5 cut(s) 92, 126, 210, 726, 881
TscAI CASTG 4 cut(s) 415, 595, 762, 1183
TseFI GTSAC 3 cut(s) 821, 1058, 1178
TseI GCWGC 3 cut(s) 749, 767, 1200
Tsp45I GTSAC 3 cut(s) 821, 1058, 1178
TspDTI ATGAA 1 cut(s) 71
TspGWI ACGGA 1 cut(s) 1121
TspRI CASTG 4 cut(s) 415, 595, 762, 1183
Van91I CCANNNNNTGG 2 cut(s) 464, 1010
VpaK11BI GGWCC 2 cut(s) 200, 760
XapI RAATTY 2 cut(s) 169, 951
XbaI TCTAGA 1 cut(s) 227
XcmI CCANNNNNNNNNTGG 2 cut(s) 344, 798
XspI CTAG 3 cut(s) 228, 287, 890
ZraI GACGTC 1 cut(s) 1226
ZrmI AGTACT 1 cut(s) 46
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.