Rmu_sc0000883.1_g000001

Adenosylhomocysteinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000883.1
OG0009985
Physical Location & Seq
Forward (+)
1 .. 1803
1803 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 806 bp
ccagtggcacattgttgttccctgctatcaatgtcaacgactctgttaccaagagcaagtttgacaacttgtatggttgccgtcactctctccctgatggtttgatgagagccactgatgttatgattgccggaaaggtttctgttgtctgtggatatggtgatgttggaaagggttgtgctgctgccctcaagcaagctggatcccgtgtgatcgtgactgagattgacccaatctgtgctcttcaggctactatggagggtcttcaggttcttccccttgaggacgttgtctctgaggctgacatctttgtcaccaccaccggaaacaaggacatcatcatggttgaccatatgaggaagatgaagaacaatgccattgtttgcaacattgggcattttgacaatgagattgacatgattggacttgagacatacccaggagtgaagcgcatcaccatcaagcctcaaacagacaggtgggtcttccctgagaccaagtctggtatcattgtgttggctgagggccgtctcatgaacttgggttgtgccactggacaccccagctttgtgatgtcctgctctttcaccaaccaagtgattgctcagcttgagttgtggaatgagaggaagagcggcaagtatgagaagaaggtctatgtcttgcccaagcaccttgatgagaaggttgctgctcttcatcttggaaagcttggagccaagctcaccaagctctccaaggaccaagccgactacatcagcgttccaattgagggtccatacaagcctgctcactacaggtactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 map00270 map01100
Pfam Domains
Protein Families
AdoHcyase AdoHcyase_NAD

Protein Analysis

267

Amino Acids

29.29

Weight (kDa)

8.08

Isoelectric Point (pI)

26.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AdoHcyase PF05221 2 - 266 2.5e-49 S-adenosyl-L-homocysteine hydrolase
AdoHcyase_NAD PF00670 22 - 185 2e-83 S-adenosyl-L-homocysteine hydrolase, NAD binding domain
2-Hacid_dh_C PF02826 41 - 131 1.3e-08 D-isomer specific 2-hydroxyacid dehydrogenase, NAD binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0009985)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G23810 AT4G13940 AT4G13940 AT4G13940 AT4G13940
fragaria_vesca FvH4_4g13280
malus_domestica MD05G1230000.v1.1 MD13G1281900.v1.1
prunus_persica Prupe.1G165200_v2.0.a1
pyrus_communis pycom420g00960 pycom420g00980
rosa_chinensis RchiOBHm_Chr4g0413571
rosa_laevigata RLG00000008212
rosa_multiflora Rmu_sc0000883.1_g000001
rosa_roxburghii Rroxscaffold_5G00357440
rosa_rugosa Rorug04G0120900
rosa_samantha Rh4AG180700 Rh4BG179900 Rh4CG191800 Rh4DG175800
rosa_wichuraiana Rw4G015090

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 310, 481
AccBSI CCGCTC 1 cut(s) 635
AciI CCGC 1 cut(s) 635
AclWI GGATC 2 cut(s) 197, 210
AcuI CTGAAG 2 cut(s) 229, 250
AfaI GTAC 1 cut(s) 802
AfiI CCNNNNNNNGG 1 cut(s) 772
AjnI CCWGG 1 cut(s) 438
AluBI AGCT 6 cut(s) 199, 566, 609, 711, 723, 732
AluI AGCT 6 cut(s) 199, 566, 609, 711, 723, 732
Alw21I GWGCWC 1 cut(s) 243
Alw26I GTCTC 4 cut(s) 297, 424, 487, 535
AlwI GGATC 2 cut(s) 197, 210
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 1 cut(s) 525
ApeKI GCWGC 3 cut(s) 181, 184, 691
Asp700I GAANNNNTTC 1 cut(s) 138
AspLEI GCGC 1 cut(s) 452
AspS9I GGNCC 3 cut(s) 525, 741, 775
AsuHPI GGTGA 5 cut(s) 172, 306, 447, 579, 717
AvaII GGWCC 2 cut(s) 741, 775
BamHI GGATCC 1 cut(s) 202
BbsI GAAGAC 2 cut(s) 256, 477
Bbv12I GWGCWC 1 cut(s) 243
BbvCI CCTCAGC 1 cut(s) 521
BbvI GCAGC 3 cut(s) 168, 171, 678
BccI CCATC 2 cut(s) 91, 466
BceAI ACGGC 2 cut(s) 65, 512
BciT130I CCWGG 1 cut(s) 440
BcoDI GTCTC 4 cut(s) 297, 424, 487, 535
BfmI CTRYAG 1 cut(s) 795
BisI GCNGC 4 cut(s) 182, 185, 636, 692
BlpI GCTNAGC 1 cut(s) 605
BlsI GCNGC 4 cut(s) 183, 186, 637, 693
Bme1390I CCNGG 1 cut(s) 440
Bme18I GGWCC 2 cut(s) 741, 775
BmgT120I GGNCC 3 cut(s) 525, 741, 775
BmiI GGNNCC 3 cut(s) 204, 717, 776
BmrFI CCNGG 1 cut(s) 440
BmsI GCATC 1 cut(s) 461
BpiI GAAGAC 2 cut(s) 256, 477
BplI GAGNNNNNCTC 2 cut(s) 707, 739
Bpu10I CCTNAGC 1 cut(s) 521
Bpu1102I GCTNAGC 1 cut(s) 605
BpuEI CTTGAG 4 cut(s) 175, 301, 448, 631
BsaI GGTCTC 1 cut(s) 487
BsaJI CCNNGG 2 cut(s) 438, 737
BsaWI WCCGGW 1 cut(s) 322
Bsc4I CCNNNNNNNGG 1 cut(s) 772
Bse1I ACTGG 1 cut(s) 558
BseBI CCWGG 1 cut(s) 440
BseDI CCNNGG 2 cut(s) 438, 737
BseLI CCNNNNNNNGG 1 cut(s) 772
BseMII CTCAG 5 cut(s) 212, 287, 482, 512, 619
BseNI ACTGG 1 cut(s) 558
BseXI GCAGC 3 cut(s) 168, 171, 678
BseYI CCCAGC 1 cut(s) 562
BshFI GGCC 1 cut(s) 527
BsiHKAI GWGCWC 1 cut(s) 243
BsiSI CCGG 2 cut(s) 131, 323
BslI CCNNNNNNNGG 1 cut(s) 772
BsmAI GTCTC 4 cut(s) 297, 424, 487, 535
BsmBI CGTCTC 1 cut(s) 535
BsnI GGCC 1 cut(s) 527
Bso31I GGTCTC 1 cut(s) 487
Bsp1286I GDGCHC 1 cut(s) 243
Bsp143I GATC 2 cut(s) 202, 212
Bsp1720I GCTNAGC 1 cut(s) 605
BspACI CCGC 1 cut(s) 635
BspANI GGCC 1 cut(s) 527
BspCNI CTCAG 5 cut(s) 213, 288, 483, 513, 618
BspHI TCATGA 1 cut(s) 533
BspLI GGNNCC 3 cut(s) 204, 717, 776
BspPI GGATC 2 cut(s) 197, 210
BspQI GCTCTTC 3 cut(s) 248, 625, 701
BspTNI GGTCTC 1 cut(s) 487
BsrBI CCGCTC 1 cut(s) 635
BsrI ACTGG 1 cut(s) 558
BssECI CCNNGG 2 cut(s) 438, 737
BssMI GATC 2 cut(s) 202, 212
BssT1I CCWWGG 1 cut(s) 737
Bst2UI CCWGG 1 cut(s) 440
Bst6I CTCTTC 3 cut(s) 248, 625, 701
BstC8I GCNNGC 2 cut(s) 197, 788
BstDEI CTNAG 5 cut(s) 221, 296, 491, 521, 605
BstHHI GCGC 1 cut(s) 452
BstKTI GATC 2 cut(s) 205, 215
BstMAI GTCTC 4 cut(s) 297, 424, 487, 535
BstMBI GATC 2 cut(s) 202, 212
BstMWI GCNNNNNNNGC 2 cut(s) 247, 729
BstNI CCWGG 1 cut(s) 440
BstSCI CCNGG 1 cut(s) 438
BstSFI CTRYAG 1 cut(s) 795
BstV1I GCAGC 3 cut(s) 168, 171, 678
BstV2I GAAGAC 2 cut(s) 256, 477
BstX2I RGATCY 1 cut(s) 202
BstYI RGATCY 1 cut(s) 202
BsuRI GGCC 1 cut(s) 527
BtsIMutI CAGTG 3 cut(s) 9, 113, 551
Cac8I GCNNGC 2 cut(s) 197, 788
CaiI CAGNNNCTG 1 cut(s) 803
CciI TCATGA 1 cut(s) 533
CfoI GCGC 1 cut(s) 452
Cfr13I GGNCC 3 cut(s) 525, 741, 775
Csp6I GTAC 1 cut(s) 801
CviAII CATG 3 cut(s) 342, 417, 534
CviQI GTAC 1 cut(s) 801
DdeI CTNAG 5 cut(s) 221, 296, 491, 521, 605
DpnI GATC 2 cut(s) 204, 214
DpnII GATC 2 cut(s) 202, 212
DrdI GACNNNNNNGTC 2 cut(s) 310, 481
DseDI GACNNNNNNGTC 2 cut(s) 310, 481
Eam1104I CTCTTC 3 cut(s) 248, 625, 701
EarI CTCTTC 3 cut(s) 248, 625, 701
Eco130I CCWWGG 1 cut(s) 737
Eco31I GGTCTC 1 cut(s) 487
Eco47I GGWCC 2 cut(s) 741, 775
Eco57I CTGAAG 2 cut(s) 229, 250
EcoRII CCWGG 1 cut(s) 438
EcoT14I CCWWGG 1 cut(s) 737
ErhI CCWWGG 1 cut(s) 737
Esp3I CGTCTC 1 cut(s) 535
FaeI CATG 3 cut(s) 345, 420, 537
FatI CATG 3 cut(s) 341, 416, 533
FauNDI CATATG 1 cut(s) 353
Fnu4HI GCNGC 4 cut(s) 182, 185, 636, 692
Fsp4HI GCNGC 4 cut(s) 182, 185, 636, 692
GlaI GCGC 1 cut(s) 451
GluI GCNGC 4 cut(s) 182, 185, 636, 692
GsaI CCCAGC 1 cut(s) 566
HaeIII GGCC 1 cut(s) 527
HapII CCGG 2 cut(s) 131, 323
HhaI GCGC 1 cut(s) 452
Hin1II CATG 3 cut(s) 345, 420, 537
Hin6I GCGC 1 cut(s) 450
HinP1I GCGC 1 cut(s) 450
HincII GTYRAC 2 cut(s) 36, 348
HindII GTYRAC 2 cut(s) 36, 348
HindIII AAGCTT 1 cut(s) 709
HinfI GANTC 1 cut(s) 40
HpaII CCGG 2 cut(s) 131, 323
HphI GGTGA 5 cut(s) 172, 306, 447, 579, 717
Hpy166II GTNNAC 2 cut(s) 36, 348
Hpy188I TCNGA 1 cut(s) 297
Hpy188III TCNNGA 2 cut(s) 216, 534
Hpy8I GTNNAC 2 cut(s) 36, 348
HpyAV CCTTC 2 cut(s) 645, 678
HpyCH4IV ACGT 1 cut(s) 287
HpyCH4V TGCA 1 cut(s) 386
HpyF10VI GCNNNNNNNGC 2 cut(s) 247, 729
HpyF3I CTNAG 5 cut(s) 221, 296, 491, 521, 605
HpySE526I ACGT 1 cut(s) 287
Hsp92II CATG 3 cut(s) 345, 420, 537
HspAI GCGC 1 cut(s) 450
Kzo9I GATC 2 cut(s) 202, 212
LguI GCTCTTC 3 cut(s) 248, 625, 701
LmnI GCTCC 1 cut(s) 715
Lsp1109I GCAGC 3 cut(s) 168, 171, 678
LweI GCATC 1 cut(s) 461
MaeII ACGT 1 cut(s) 287
MaeIII GTNAC 4 cut(s) 45, 82, 216, 312
MalI GATC 2 cut(s) 204, 214
MbiI CCGCTC 1 cut(s) 635
MboI GATC 2 cut(s) 202, 212
MboII GAAGA 9 cut(s) 235, 256, 265, 372, 378, 477, 642, 660, 688
MfeI CAATTG 1 cut(s) 767
MflI RGATCY 1 cut(s) 202
MhlI GDGCHC 1 cut(s) 243
MluCI AATT 1 cut(s) 767
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 147
MnlI CCTC 9 cut(s) 199, 252, 276, 291, 350, 476, 516, 620, 765
MroXI GAANNNNTTC 1 cut(s) 138
MslI CAYNNNNRTG 2 cut(s) 340, 677
MspI CCGG 2 cut(s) 131, 323
MspR9I CCNGG 1 cut(s) 440
MunI CAATTG 1 cut(s) 767
MvaI CCWGG 1 cut(s) 440
MwoI GCNNNNNNNGC 2 cut(s) 247, 729
NdeI CATATG 1 cut(s) 353
NdeII GATC 2 cut(s) 202, 212
NlaIII CATG 3 cut(s) 345, 420, 537
NlaIV GGNNCC 3 cut(s) 204, 717, 776
NmuCI GTSAC 3 cut(s) 82, 216, 312
PagI TCATGA 1 cut(s) 533
PciSI GCTCTTC 3 cut(s) 248, 625, 701
PdmI GAANNNNTTC 1 cut(s) 138
PflFI GACNNNGTC 2 cut(s) 289, 498
PkrI GCNGC 4 cut(s) 183, 186, 637, 693
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 1 cut(s) 438
PspFI CCCAGC 1 cut(s) 562
PspGI CCWGG 1 cut(s) 438
PspN4I GGNNCC 3 cut(s) 204, 717, 776
PspPI GGNCC 3 cut(s) 525, 741, 775
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 1 cut(s) 202
PsyI GACNNNGTC 2 cut(s) 289, 498
RsaI GTAC 1 cut(s) 802
RsaNI GTAC 1 cut(s) 801
RseI CAYNNNNRTG 2 cut(s) 340, 677
SapI GCTCTTC 3 cut(s) 248, 625, 701
SatI GCNGC 4 cut(s) 182, 185, 636, 692
Sau3AI GATC 2 cut(s) 202, 212
Sau96I GGNCC 3 cut(s) 525, 741, 775
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 1 cut(s) 440
SduI GDGCHC 1 cut(s) 243
SfaNI GCATC 1 cut(s) 461
SfcI CTRYAG 1 cut(s) 795
SinI GGWCC 2 cut(s) 741, 775
SmiMI CAYNNNNRTG 2 cut(s) 340, 677
SmlI CTYRAG 4 cut(s) 190, 280, 427, 610
SmoI CTYRAG 4 cut(s) 190, 280, 427, 610
Sse9I AATT 1 cut(s) 767
SsiI CCGC 1 cut(s) 635
StyD4I CCNGG 1 cut(s) 438
StyI CCWWGG 1 cut(s) 737
TaiI ACGT 1 cut(s) 290
TasI AATT 1 cut(s) 767
TauI GCSGC 1 cut(s) 638
TscAI CASTG 2 cut(s) 120, 558
TseFI GTSAC 3 cut(s) 82, 216, 312
TseI GCWGC 3 cut(s) 181, 184, 691
Tsp45I GTSAC 3 cut(s) 82, 216, 312
TspDTI ATGAA 3 cut(s) 379, 550, 688
TspRI CASTG 2 cut(s) 120, 558
Tth111I GACNNNGTC 2 cut(s) 289, 498
VpaK11BI GGWCC 2 cut(s) 741, 775
XmnI GAANNNNTTC 1 cut(s) 138
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.