Rmu_sc0001207.1_g000082

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001207.1
OG0016389
Physical Location & Seq
Forward (+)
349281 .. 350252
972 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 972 bp
atgggatctttgagtgcagaaattcacgaggacaagaaattatgcgaggactggtctgcgcttcatatgttggtagaggtttcctgccatgttggcgaagaagaaagaaagggtatgtgcgtgttatctttggtgagtcaacaggtatgccctaagaaacaaaggtcgccaccccgtggtgaaagttcgaaaatgacgagccctatccctaatattgcttctggttcttctgatcaagaaaatagaagaattgatgataagggtttgaaaagaaaaaggaagccatgtgttgattgtcataatgataaagacttggatgccgcggcgaagaagaagaagaagagaacgtatatgatgaaggtggacatgggtgtttatcaaccacctcctgatttgcccgaaaagtacaagcaaatggtgaatgggccaaatgcggcattggtgatacaaaaccagttgtttacgagtgatgtgaaccctggcctgaaccggctgacattgccaccaaaacaaattttgtgcaaggagttgctgagacctaatgagatagaaatccttaggccgaaggatgctttgcttaaagttccatttatagacccagagctgagaaaggaagagatttcactgagtttatggaagctgtcgagctcaaatgaaaggtcccttgtgttcaaatctgaatggaatcatattgttaagaagaacgatctgggtgaaggtgatctgatccaaatctggtcatttcgggatagcaacgatcaacttcagttggcacttgttgtggtgaagaaagccgagaagcgcaaaagtggtgaggaagtgagaagcaccgcaagaagtagtagtgtgagcaatgtcagtgaggacagtgcaagtgcaagccacagtggaagaaaatgtctcaaaatccgatttaaatgtaatgctagcaggagtgagagtactagtacatgtgaattattgagtcactga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

323

Amino Acids

36.36

Weight (kDa)

9.11

Isoelectric Point (pI)

59.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 137 - 234 3.7e-11 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13550
prunus_persica Prupe.2G161700_v2.0.a1 Prupe.2G162000_v2.0.a1
rosa_laevigata RLG00000028289
rosa_multiflora Rmu_sc0001207.1_g000082 Rmu_sc0011028.1_g000001
rosa_roxburghii Rroxscaffold_4G00302050
rosa_rugosa Rorug01G0233600
rosa_samantha Rh1AG247500 Rh1BG217300 Rh1CG231000 Rh1DG243400
rosa_wichuraiana Rw0G018090 Rw1G021410 Rw1G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 176
AccII CGCG 1 cut(s) 323
AciI CCGC 4 cut(s) 321, 323, 434, 831
AclWI GGATC 2 cut(s) 13, 721
AcsI RAATTY 2 cut(s) 21, 513
AcuI CTGAAG 1 cut(s) 749
AdeI CACNNNGTG 1 cut(s) 176
AfaI GTAC 3 cut(s) 407, 943, 949
AfiI CCNNNNNNNGG 1 cut(s) 176
AflIII ACRYGT 1 cut(s) 950
AgsI TTSAA 2 cut(s) 268, 673
AhlI ACTAGT 1 cut(s) 944
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 3 cut(s) 604, 640, 648
AluI AGCT 3 cut(s) 604, 640, 648
Alw21I GWGCWC 1 cut(s) 650
Alw26I GTCTC 2 cut(s) 529, 905
AlwI GGATC 2 cut(s) 13, 721
AoxI GGCC 3 cut(s) 425, 481, 560
ApoI RAATTY 2 cut(s) 21, 513
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AspLEI GCGC 2 cut(s) 61, 804
AspS9I GGNCC 2 cut(s) 425, 660
AsuHPI GGTGA 8 cut(s) 145, 191, 430, 454, 725, 731, 796, 824
AsuII TTCGAA 1 cut(s) 188
AsuNHI GCTAGC 1 cut(s) 926
AvaII GGWCC 1 cut(s) 660
AxyI CCTNAGG 1 cut(s) 557
BanII GRGCYC 2 cut(s) 203, 650
BauI CACGAG 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 650
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 232
BcoDI GTCTC 2 cut(s) 529, 905
BcuI ACTAGT 1 cut(s) 944
BfaI CTAG 2 cut(s) 927, 945
BglI GCCNNNNNGGC 1 cut(s) 93
BisI GCNGC 3 cut(s) 321, 324, 435
BlsI GCNGC 3 cut(s) 322, 325, 436
BmcAI AGTACT 1 cut(s) 943
Bme1390I CCNGG 1 cut(s) 480
Bme18I GGWCC 1 cut(s) 660
BmgT120I GGNCC 2 cut(s) 425, 660
BmiI GGNNCC 1 cut(s) 662
BmrFI CCNGG 1 cut(s) 480
BmsI GCATC 2 cut(s) 307, 559
BmtI GCTAGC 1 cut(s) 930
Bpu14I TTCGAA 1 cut(s) 188
BsaBI GATNNNNATC 2 cut(s) 551, 731
BsaI GGTCTC 1 cut(s) 529
BsaJI CCNNGG 3 cut(s) 175, 321, 478
BsaXI ACNNNNNCTCC 2 cut(s) 925, 955
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse118I RCCGGY 1 cut(s) 489
Bse1I ACTGG 2 cut(s) 56, 454
Bse21I CCTNAGG 1 cut(s) 557
Bse3DI GCAATG 2 cut(s) 497, 859
Bse8I GATNNNNATC 2 cut(s) 551, 731
BseBI CCWGG 1 cut(s) 480
BseDI CCNNGG 3 cut(s) 175, 321, 478
BseGI GGATG 2 cut(s) 322, 574
BseJI GATNNNNATC 2 cut(s) 551, 731
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 2 cut(s) 497, 859
BseMII CTCAG 3 cut(s) 524, 596, 617
BseNI ACTGG 2 cut(s) 56, 454
BsgI GTGCAG 1 cut(s) 36
Bsh1236I CGCG 1 cut(s) 323
BshFI GGCC 3 cut(s) 427, 483, 562
BsiHKAI GWGCWC 1 cut(s) 650
BsiSI CCGG 1 cut(s) 490
BslFI GGGAC 1 cut(s) 646
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 2 cut(s) 529, 905
BsmFI GGGAC 1 cut(s) 646
BsnI GGCC 3 cut(s) 427, 483, 562
Bso31I GGTCTC 1 cut(s) 529
Bsp119I TTCGAA 1 cut(s) 188
Bsp1286I GDGCHC 2 cut(s) 203, 650
Bsp143I GATC 6 cut(s) 5, 232, 706, 721, 726, 757
BspACI CCGC 4 cut(s) 321, 323, 434, 831
BspANI GGCC 3 cut(s) 427, 483, 562
BspCNI CTCAG 3 cut(s) 525, 597, 618
BspFNI CGCG 1 cut(s) 323
BspLI GGNNCC 1 cut(s) 662
BspOI GCTAGC 1 cut(s) 930
BspPI GGATC 2 cut(s) 13, 721
BspT104I TTCGAA 1 cut(s) 188
BspTNI GGTCTC 1 cut(s) 529
BsrDI GCAATG 2 cut(s) 497, 859
BsrFI RCCGGY 1 cut(s) 489
BsrI ACTGG 2 cut(s) 56, 454
BssAI RCCGGY 1 cut(s) 489
BssECI CCNNGG 3 cut(s) 175, 321, 478
BssMI GATC 6 cut(s) 5, 232, 706, 721, 726, 757
BssSI CACGAG 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 2 cut(s) 869, 887
Bst6I CTCTTC 2 cut(s) 335, 609
BstBI TTCGAA 1 cut(s) 188
BstC8I GCNNGC 2 cut(s) 880, 928
BstDEI CTNAG 5 cut(s) 153, 533, 557, 605, 626
BstDSI CCRYGG 2 cut(s) 175, 321
BstF5I GGATG 2 cut(s) 322, 574
BstFNI CGCG 1 cut(s) 323
BstHHI GCGC 2 cut(s) 61, 804
BstKTI GATC 6 cut(s) 8, 235, 709, 724, 729, 760
BstMAI GTCTC 2 cut(s) 529, 905
BstMBI GATC 6 cut(s) 5, 232, 706, 721, 726, 757
BstMWI GCNNNNNNNGC 2 cut(s) 93, 499
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 1 cut(s) 954
BstSCI CCNGG 1 cut(s) 478
BstUI CGCG 1 cut(s) 323
BstX2I RGATCY 1 cut(s) 5
BstYI RGATCY 1 cut(s) 5
Bsu36I CCTNAGG 1 cut(s) 557
BsuRI GGCC 3 cut(s) 427, 483, 562
BtgI CCRYGG 2 cut(s) 175, 321
BtsCI GGATG 2 cut(s) 322, 574
BtsIMutI CAGTG 5 cut(s) 623, 865, 874, 892, 967
Cac8I GCNNGC 2 cut(s) 880, 928
CfoI GCGC 2 cut(s) 61, 804
Cfr10I RCCGGY 1 cut(s) 489
Cfr13I GGNCC 2 cut(s) 425, 660
Cfr42I CCGCGG 1 cut(s) 324
Csp6I GTAC 3 cut(s) 406, 942, 948
CviAII CATG 4 cut(s) 89, 285, 367, 951
CviQI GTAC 3 cut(s) 406, 942, 948
DdeI CTNAG 5 cut(s) 153, 533, 557, 605, 626
DpnI GATC 6 cut(s) 7, 234, 708, 723, 728, 759
DpnII GATC 6 cut(s) 5, 232, 706, 721, 726, 757
DraI TTTAAA 1 cut(s) 916
DraIII CACNNNGTG 1 cut(s) 176
Eam1104I CTCTTC 2 cut(s) 335, 609
EarI CTCTTC 2 cut(s) 335, 609
Ecl136II GAGCTC 1 cut(s) 648
Eco24I GRGCYC 2 cut(s) 203, 650
Eco31I GGTCTC 1 cut(s) 529
Eco47I GGWCC 1 cut(s) 660
Eco53kI GAGCTC 1 cut(s) 648
Eco57I CTGAAG 1 cut(s) 749
Eco81I CCTNAGG 1 cut(s) 557
EcoICRI GAGCTC 1 cut(s) 648
EcoO109I RGGNCCY 1 cut(s) 660
EcoRII CCWGG 1 cut(s) 478
EcoT38I GRGCYC 2 cut(s) 203, 650
FaeI CATG 4 cut(s) 92, 288, 370, 954
FaqI GGGAC 1 cut(s) 646
FatI CATG 4 cut(s) 88, 284, 366, 950
FauNDI CATATG 1 cut(s) 66
FbaI TGATCA 1 cut(s) 232
Fnu4HI GCNGC 3 cut(s) 321, 324, 435
FokI GGATG 2 cut(s) 329, 581
FriOI GRGCYC 2 cut(s) 203, 650
Fsp4HI GCNGC 3 cut(s) 321, 324, 435
FspBI CTAG 2 cut(s) 927, 945
GlaI GCGC 2 cut(s) 60, 803
GluI GCNGC 3 cut(s) 321, 324, 435
HaeIII GGCC 3 cut(s) 427, 483, 562
HapII CCGG 1 cut(s) 490
HhaI GCGC 2 cut(s) 61, 804
Hin1II CATG 4 cut(s) 92, 288, 370, 954
Hin6I GCGC 2 cut(s) 59, 802
HinP1I GCGC 2 cut(s) 59, 802
HincII GTYRAC 1 cut(s) 140
HindII GTYRAC 1 cut(s) 140
HinfI GANTC 3 cut(s) 136, 685, 964
HpaII CCGG 1 cut(s) 490
HphI GGTGA 8 cut(s) 145, 191, 430, 454, 725, 731, 796, 824
Hpy166II GTNNAC 4 cut(s) 140, 364, 462, 475
Hpy188I TCNGA 4 cut(s) 232, 679, 726, 911
Hpy188III TCNNGA 4 cut(s) 26, 236, 389, 746
Hpy8I GTNNAC 4 cut(s) 140, 364, 462, 475
HpyAV CCTTC 3 cut(s) 352, 559, 710
HpyCH4III ACNGT 2 cut(s) 869, 887
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 4 cut(s) 17, 522, 872, 878
HpyF10VI GCNNNNNNNGC 2 cut(s) 93, 499
HpyF3I CTNAG 5 cut(s) 153, 533, 557, 605, 626
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 4 cut(s) 92, 288, 370, 954
HspAI GCGC 2 cut(s) 59, 802
Ksp22I TGATCA 1 cut(s) 232
KspI CCGCGG 1 cut(s) 324
Kzo9I GATC 6 cut(s) 5, 232, 706, 721, 726, 757
LweI GCATC 2 cut(s) 307, 559
MaeI CTAG 2 cut(s) 927, 945
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 1 cut(s) 965
MalI GATC 6 cut(s) 7, 234, 708, 723, 728, 759
MboI GATC 6 cut(s) 5, 232, 706, 721, 726, 757
MflI RGATCY 1 cut(s) 5
MhlI GDGCHC 2 cut(s) 203, 650
MluCI AATT 5 cut(s) 21, 38, 249, 513, 956
MlyI GAGTC 1 cut(s) 145
MnlI CCTC 6 cut(s) 22, 40, 70, 396, 808, 856
MseI TTAA 3 cut(s) 579, 696, 915
MspA1I CMGCKG 1 cut(s) 323
MspI CCGG 1 cut(s) 490
MspR9I CCNGG 1 cut(s) 480
MvaI CCWGG 1 cut(s) 480
MvnI CGCG 1 cut(s) 323
MwoI GCNNNNNNNGC 2 cut(s) 93, 499
NdeI CATATG 1 cut(s) 66
NdeII GATC 6 cut(s) 5, 232, 706, 721, 726, 757
NheI GCTAGC 1 cut(s) 926
NlaIII CATG 4 cut(s) 92, 288, 370, 954
NlaIV GGNNCC 1 cut(s) 662
NmeAIII GCCGAG 1 cut(s) 820
NmuCI GTSAC 1 cut(s) 965
NspI RCATGY 1 cut(s) 954
NspV TTCGAA 1 cut(s) 188
PciI ACATGT 1 cut(s) 950
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PfeI GAWTC 1 cut(s) 685
PflMI CCANNNNNTGG 1 cut(s) 176
PkrI GCNGC 3 cut(s) 322, 325, 436
PleI GAGTC 1 cut(s) 144
PpsI GAGTC 1 cut(s) 144
PpuMI RGGWCCY 1 cut(s) 660
PscI ACATGT 1 cut(s) 950
Psp124BI GAGCTC 1 cut(s) 650
Psp5II RGGWCCY 1 cut(s) 660
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 662
PspPI GGNCC 2 cut(s) 425, 660
PspPPI RGGWCCY 1 cut(s) 660
PsuI RGATCY 1 cut(s) 5
RsaI GTAC 3 cut(s) 407, 943, 949
RsaNI GTAC 3 cut(s) 406, 942, 948
SacI GAGCTC 1 cut(s) 650
SacII CCGCGG 1 cut(s) 324
SaqAI TTAA 3 cut(s) 579, 696, 915
SatI GCNGC 3 cut(s) 321, 324, 435
Sau3AI GATC 6 cut(s) 5, 232, 706, 721, 726, 757
Sau96I GGNCC 2 cut(s) 425, 660
ScaI AGTACT 1 cut(s) 943
SchI GAGTC 1 cut(s) 145
ScrFI CCNGG 1 cut(s) 480
SduI GDGCHC 2 cut(s) 203, 650
SfaNI GCATC 2 cut(s) 307, 559
Sfr303I CCGCGG 1 cut(s) 324
SfuI TTCGAA 1 cut(s) 188
SgrBI CCGCGG 1 cut(s) 324
SinI GGWCC 1 cut(s) 660
SmiI ATTTAAAT 1 cut(s) 916
SpeI ACTAGT 1 cut(s) 944
Sse9I AATT 5 cut(s) 21, 38, 249, 513, 956
SsiI CCGC 4 cut(s) 321, 323, 434, 831
SspI AATATT 1 cut(s) 214
SspMI CTAG 2 cut(s) 927, 945
SstI GAGCTC 1 cut(s) 650
StyD4I CCNGG 1 cut(s) 478
SwaI ATTTAAAT 1 cut(s) 916
TaaI ACNGT 2 cut(s) 869, 887
TaiI ACGT 1 cut(s) 350
TaqI TCGA 2 cut(s) 188, 644
TasI AATT 5 cut(s) 21, 38, 249, 513, 956
TatI WGTACW 3 cut(s) 405, 941, 947
TauI GCSGC 3 cut(s) 323, 326, 437
TfiI GAWTC 1 cut(s) 685
Tru1I TTAA 3 cut(s) 579, 696, 915
Tru9I TTAA 3 cut(s) 579, 696, 915
TscAI CASTG 4 cut(s) 630, 865, 874, 892
TseFI GTSAC 1 cut(s) 965
Tsp45I GTSAC 1 cut(s) 965
TspDTI ATGAA 3 cut(s) 53, 371, 669
TspRI CASTG 4 cut(s) 630, 865, 874, 892
Van91I CCANNNNNTGG 1 cut(s) 176
VpaK11BI GGWCC 1 cut(s) 660
XapI RAATTY 2 cut(s) 21, 513
XceI RCATGY 1 cut(s) 954
XspI CTAG 2 cut(s) 927, 945
ZrmI AGTACT 1 cut(s) 943
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.