Rroxscaffold_4G00302050

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016389
Physical Location & Seq
Forward (+)
22399646 .. 22400674
1029 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00302050.1

Sequence Viewer

Length: 1029 bp
ATGGGTACACTGGTCAGTTCTTCAAATCTCTCATATCTTCGAGAACAAGAAGAAAAGATGGGATCTTTGAGTACGGAAATCCTCCAGGACAAGAAATTATGCGAGGACTGGTCTGCGCTTCATATGTTGGTAGAGGTTTCCTGCCATGTTAGCGAAGAAGAAAAAAAGGGTGTGTGTGTGTTATCCTTGTTGAGTCAACAGGTATGCCCAAAGAAAGAAAGGTCGCCACCCCGCGGTGCAAGTTCGAAAATGACGAGCCCTATCCCTAATATTGCTTCTGGTTCTTCTGATCAAGAAAATAGAAGAATTGATGATAAGGGTTTGAAAAGAAAAAGGAACCCATGTGTTGATTGGCATAATGATAAAGACTCGGAGACCGCGGCGAAGAAGAAGAAGAAGAGAAGATATATGATGAAGGTGGACATGGGTGTTTATCAACCACCCCCCGATTTGCCCGAAAAGTACAAGCAAATGGTGAATGGGGCAAATGCGGCATTGGTGATACAAAAGCAGTTGTTTACGAGTGATGTGAACCCTGACCTGAACCGGCTGACATTGCCTCCAAAACAAATTTTGTGCAAGGAGTTGCTGAGACCTAATGAGATAGAAATCCTTAAGCCGAAGGAAGCTTTGCTTAAAGTTCCATTTATAGACCCAGAGCTCAGAAAGGAAGAGATTTCACTGAGTTTATGGAAGCTGTCGAGCTCAAATGAAAGGTCCCTTGTGTTCAAATCTAAATGGAATCATATTGTTAAGAAGAACGATCTGGGTGAAGGTGATCTGATCCAAATCTGGTCATTTCGGGATAGCAACGATCAACTTCAGTTGGCACTTGTTGTGGTGAAGAGAGCCGAGAAGCGCAAAAATGGCGAGGAAGTGAGAAGCACCGCAAGTAGTAGTAGTGTGAGCAATGTCAGTGAGGACAGTGCAAGTGCAAGCCACAGTGGAAGAAAATGTCTCAAAATCAGATTTAAATGTAATGCTAGCAGGAGTGAGAGTACTAGTACATGTGAATTATTGAGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

342

Amino Acids

38.6

Weight (kDa)

9.18

Isoelectric Point (pI)

64.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 155 - 253 2.5e-12 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13550
prunus_persica Prupe.2G161700_v2.0.a1 Prupe.2G162000_v2.0.a1
rosa_laevigata RLG00000028289
rosa_multiflora Rmu_sc0001207.1_g000082 Rmu_sc0011028.1_g000001
rosa_roxburghii Rroxscaffold_4G00302050
rosa_rugosa Rorug01G0233600
rosa_samantha Rh1AG247500 Rh1BG217300 Rh1CG231000 Rh1DG243400
rosa_wichuraiana Rw0G018090 Rw1G021410 Rw1G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 234, 380
AciI CCGC 6 cut(s) 232, 234, 378, 380, 491, 888
AclWI GGATC 2 cut(s) 70, 778
AcsI RAATTY 1 cut(s) 570
AcuI CTGAAG 1 cut(s) 806
AfaI GTAC 5 cut(s) 7, 73, 464, 1000, 1006
AfiI CCNNNNNNNGG 1 cut(s) 233
AflII CTTAAG 1 cut(s) 614
AflIII ACRYGT 1 cut(s) 1007
AgsI TTSAA 3 cut(s) 24, 325, 730
AhlI ACTAGT 1 cut(s) 1001
AjnI CCWGG 1 cut(s) 84
AluBI AGCT 4 cut(s) 629, 661, 697, 705
AluI AGCT 4 cut(s) 629, 661, 697, 705
Alw21I GWGCWC 2 cut(s) 663, 707
Alw26I GTCTC 3 cut(s) 368, 586, 962
AlwI GGATC 2 cut(s) 70, 778
ApoI RAATTY 1 cut(s) 570
ArsI GACNNNNNNTTYG 2 cut(s) 701, 733
AspLEI GCGC 2 cut(s) 118, 861
AspS9I GGNCC 1 cut(s) 717
AsuHPI GGTGA 5 cut(s) 487, 511, 782, 788, 853
AsuII TTCGAA 1 cut(s) 245
AsuNHI GCTAGC 1 cut(s) 983
AvaII GGWCC 1 cut(s) 717
BaeI ACNNNNGTAYC 1 cut(s) 30
BanII GRGCYC 3 cut(s) 260, 663, 707
Bbv12I GWGCWC 2 cut(s) 663, 707
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 86
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 3 cut(s) 368, 586, 962
BcuI ACTAGT 1 cut(s) 1001
BfaI CTAG 2 cut(s) 984, 1002
BfrI CTTAAG 1 cut(s) 614
BisI GCNGC 2 cut(s) 381, 492
BlsI GCNGC 2 cut(s) 382, 493
BmcAI AGTACT 1 cut(s) 1000
Bme1390I CCNGG 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 717
BmgT120I GGNCC 1 cut(s) 717
BmiI GGNNCC 2 cut(s) 338, 719
BmrFI CCNGG 1 cut(s) 86
BmtI GCTAGC 1 cut(s) 987
BpmI CTGGAG 1 cut(s) 68
Bpu14I TTCGAA 1 cut(s) 245
BsaBI GATNNNNATC 2 cut(s) 608, 788
BsaI GGTCTC 2 cut(s) 368, 586
BsaJI CCNNGG 2 cut(s) 232, 378
BsaXI ACNNNNNCTCC 4 cut(s) 544, 574, 982, 1012
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 546
Bse1I ACTGG 2 cut(s) 15, 113
Bse3DI GCAATG 2 cut(s) 554, 916
Bse8I GATNNNNATC 2 cut(s) 608, 788
BseBI CCWGG 1 cut(s) 86
BseDI CCNNGG 2 cut(s) 232, 378
BseJI GATNNNNATC 2 cut(s) 608, 788
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 2 cut(s) 554, 916
BseMII CTCAG 3 cut(s) 581, 674, 676
BseNI ACTGG 2 cut(s) 15, 113
Bsh1236I CGCG 2 cut(s) 234, 380
BsiHKAI GWGCWC 2 cut(s) 663, 707
BsiSI CCGG 1 cut(s) 547
BslFI GGGAC 1 cut(s) 703
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 3 cut(s) 368, 586, 962
BsmFI GGGAC 1 cut(s) 703
Bso31I GGTCTC 2 cut(s) 368, 586
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 3 cut(s) 260, 663, 707
Bsp143I GATC 6 cut(s) 62, 289, 763, 778, 783, 814
BspACI CCGC 6 cut(s) 232, 234, 378, 380, 491, 888
BspCNI CTCAG 3 cut(s) 582, 675, 675
BspFNI CGCG 2 cut(s) 234, 380
BspLI GGNNCC 2 cut(s) 338, 719
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 2 cut(s) 70, 778
BspT104I TTCGAA 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 614
BspTNI GGTCTC 2 cut(s) 368, 586
BsrDI GCAATG 2 cut(s) 554, 916
BsrFI RCCGGY 1 cut(s) 546
BsrI ACTGG 2 cut(s) 15, 113
BssAI RCCGGY 1 cut(s) 546
BssECI CCNNGG 2 cut(s) 232, 378
BssMI GATC 6 cut(s) 62, 289, 763, 778, 783, 814
Bst2UI CCWGG 1 cut(s) 86
Bst4CI ACNGT 2 cut(s) 926, 944
Bst6I CTCTTC 3 cut(s) 392, 666, 839
BstAFI CTTAAG 1 cut(s) 614
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 937, 985
BstDEI CTNAG 3 cut(s) 590, 662, 683
BstDSI CCRYGG 2 cut(s) 232, 378
BstFNI CGCG 2 cut(s) 234, 380
BstHHI GCGC 2 cut(s) 118, 861
BstKTI GATC 6 cut(s) 65, 292, 766, 781, 786, 817
BstMAI GTCTC 3 cut(s) 368, 586, 962
BstMBI GATC 6 cut(s) 62, 289, 763, 778, 783, 814
BstMWI GCNNNNNNNGC 4 cut(s) 150, 491, 556, 867
BstNI CCWGG 1 cut(s) 86
BstNSI RCATGY 1 cut(s) 1011
BstSCI CCNGG 1 cut(s) 84
BstUI CGCG 2 cut(s) 234, 380
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BtgI CCRYGG 2 cut(s) 232, 378
BtsIMutI CAGTG 5 cut(s) 8, 680, 922, 931, 949
Cac8I GCNNGC 2 cut(s) 937, 985
CfoI GCGC 2 cut(s) 118, 861
Cfr10I RCCGGY 1 cut(s) 546
Cfr13I GGNCC 1 cut(s) 717
Cfr42I CCGCGG 2 cut(s) 235, 381
Csp6I GTAC 5 cut(s) 6, 72, 463, 999, 1005
CviAII CATG 4 cut(s) 146, 342, 424, 1008
CviJI RGCY 9 cut(s) 258, 550, 619, 629, 661, 697, 705, 851, 939
CviKI_1 RGCY 9 cut(s) 258, 550, 619, 629, 661, 697, 705, 851, 939
CviQI GTAC 5 cut(s) 6, 72, 463, 999, 1005
DdeI CTNAG 3 cut(s) 590, 662, 683
DpnI GATC 6 cut(s) 64, 291, 765, 780, 785, 816
DpnII GATC 6 cut(s) 62, 289, 763, 778, 783, 814
DraI TTTAAA 1 cut(s) 973
Eam1104I CTCTTC 3 cut(s) 392, 666, 839
EarI CTCTTC 3 cut(s) 392, 666, 839
Ecl136II GAGCTC 2 cut(s) 661, 705
Eco24I GRGCYC 3 cut(s) 260, 663, 707
Eco31I GGTCTC 2 cut(s) 368, 586
Eco47I GGWCC 1 cut(s) 717
Eco53kI GAGCTC 2 cut(s) 661, 705
Eco57I CTGAAG 1 cut(s) 806
EcoICRI GAGCTC 2 cut(s) 661, 705
EcoO109I RGGNCCY 1 cut(s) 717
EcoRII CCWGG 1 cut(s) 84
EcoT38I GRGCYC 3 cut(s) 260, 663, 707
FaeI CATG 4 cut(s) 149, 345, 427, 1011
FalI AAGNNNNNCTT 2 cut(s) 618, 650
FaqI GGGAC 1 cut(s) 703
FatI CATG 4 cut(s) 145, 341, 423, 1007
FauI CCCGC 1 cut(s) 239
FauNDI CATATG 1 cut(s) 123
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 2 cut(s) 381, 492
FriOI GRGCYC 3 cut(s) 260, 663, 707
Fsp4HI GCNGC 2 cut(s) 381, 492
FspBI CTAG 2 cut(s) 984, 1002
GlaI GCGC 2 cut(s) 117, 860
GluI GCNGC 2 cut(s) 381, 492
GsuI CTGGAG 1 cut(s) 68
HapII CCGG 1 cut(s) 547
HhaI GCGC 2 cut(s) 118, 861
Hin1II CATG 4 cut(s) 149, 345, 427, 1011
Hin6I GCGC 2 cut(s) 116, 859
HinP1I GCGC 2 cut(s) 116, 859
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HindIII AAGCTT 1 cut(s) 627
HinfI GANTC 4 cut(s) 193, 368, 742, 1021
HpaII CCGG 1 cut(s) 547
HphI GGTGA 5 cut(s) 487, 511, 782, 788, 853
Hpy166II GTNNAC 5 cut(s) 8, 197, 421, 519, 532
Hpy188I TCNGA 5 cut(s) 289, 373, 665, 783, 968
Hpy188III TCNNGA 3 cut(s) 41, 293, 803
Hpy8I GTNNAC 5 cut(s) 8, 197, 421, 519, 532
HpyAV CCTTC 3 cut(s) 409, 616, 767
HpyCH4III ACNGT 2 cut(s) 926, 944
HpyCH4V TGCA 4 cut(s) 239, 579, 929, 935
HpyF10VI GCNNNNNNNGC 4 cut(s) 150, 491, 556, 867
HpyF3I CTNAG 3 cut(s) 590, 662, 683
Hsp92II CATG 4 cut(s) 149, 345, 427, 1011
HspAI GCGC 2 cut(s) 116, 859
Ksp22I TGATCA 1 cut(s) 289
KspI CCGCGG 2 cut(s) 235, 381
Kzo9I GATC 6 cut(s) 62, 289, 763, 778, 783, 814
MaeI CTAG 2 cut(s) 984, 1002
MalI GATC 6 cut(s) 64, 291, 765, 780, 785, 816
MboI GATC 6 cut(s) 62, 289, 763, 778, 783, 814
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 3 cut(s) 260, 663, 707
MluCI AATT 4 cut(s) 95, 306, 570, 1013
MlyI GAGTC 2 cut(s) 202, 362
MnlI CCTC 6 cut(s) 92, 97, 127, 570, 865, 913
MseI TTAA 4 cut(s) 615, 636, 753, 972
MspA1I CMGCKG 2 cut(s) 234, 380
MspCI CTTAAG 1 cut(s) 614
MspI CCGG 1 cut(s) 547
MspR9I CCNGG 1 cut(s) 86
MvaI CCWGG 1 cut(s) 86
MvnI CGCG 2 cut(s) 234, 380
MwoI GCNNNNNNNGC 4 cut(s) 150, 491, 556, 867
NdeI CATATG 1 cut(s) 123
NdeII GATC 6 cut(s) 62, 289, 763, 778, 783, 814
NheI GCTAGC 1 cut(s) 983
NlaIII CATG 4 cut(s) 149, 345, 427, 1011
NlaIV GGNNCC 2 cut(s) 338, 719
NmeAIII GCCGAG 1 cut(s) 877
NspI RCATGY 1 cut(s) 1011
NspV TTCGAA 1 cut(s) 245
PciI ACATGT 1 cut(s) 1007
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PfeI GAWTC 1 cut(s) 742
PfoI TCCNGGA 1 cut(s) 84
PkrI GCNGC 2 cut(s) 382, 493
PleI GAGTC 2 cut(s) 201, 362
PpsI GAGTC 2 cut(s) 201, 362
PpuMI RGGWCCY 1 cut(s) 717
PscI ACATGT 1 cut(s) 1007
Psp124BI GAGCTC 2 cut(s) 663, 707
Psp5II RGGWCCY 1 cut(s) 717
Psp6I CCWGG 1 cut(s) 84
PspGI CCWGG 1 cut(s) 84
PspN4I GGNNCC 2 cut(s) 338, 719
PspPI GGNCC 1 cut(s) 717
PspPPI RGGWCCY 1 cut(s) 717
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 5 cut(s) 7, 73, 464, 1000, 1006
RsaNI GTAC 5 cut(s) 6, 72, 463, 999, 1005
SacI GAGCTC 2 cut(s) 663, 707
SacII CCGCGG 2 cut(s) 235, 381
SaqAI TTAA 4 cut(s) 615, 636, 753, 972
SatI GCNGC 2 cut(s) 381, 492
Sau3AI GATC 6 cut(s) 62, 289, 763, 778, 783, 814
Sau96I GGNCC 1 cut(s) 717
ScaI AGTACT 1 cut(s) 1000
SchI GAGTC 2 cut(s) 202, 362
ScrFI CCNGG 1 cut(s) 86
SduI GDGCHC 3 cut(s) 260, 663, 707
Sfr303I CCGCGG 2 cut(s) 235, 381
SfuI TTCGAA 1 cut(s) 245
SgrBI CCGCGG 2 cut(s) 235, 381
SinI GGWCC 1 cut(s) 717
SmiI ATTTAAAT 1 cut(s) 973
SmlI CTYRAG 1 cut(s) 614
SmoI CTYRAG 1 cut(s) 614
SpeI ACTAGT 1 cut(s) 1001
Sse9I AATT 4 cut(s) 95, 306, 570, 1013
SsiI CCGC 6 cut(s) 232, 234, 378, 380, 491, 888
SspI AATATT 1 cut(s) 271
SspMI CTAG 2 cut(s) 984, 1002
SstI GAGCTC 2 cut(s) 663, 707
StyD4I CCNGG 1 cut(s) 84
SwaI ATTTAAAT 1 cut(s) 973
TaaI ACNGT 2 cut(s) 926, 944
TaqI TCGA 3 cut(s) 40, 245, 701
TasI AATT 4 cut(s) 95, 306, 570, 1013
TatI WGTACW 3 cut(s) 462, 998, 1004
TauI GCSGC 2 cut(s) 383, 494
TfiI GAWTC 1 cut(s) 742
Tru1I TTAA 4 cut(s) 615, 636, 753, 972
Tru9I TTAA 4 cut(s) 615, 636, 753, 972
TscAI CASTG 5 cut(s) 15, 687, 922, 931, 949
TspDTI ATGAA 3 cut(s) 110, 428, 726
TspGWI ACGGA 1 cut(s) 89
TspRI CASTG 5 cut(s) 15, 687, 922, 931, 949
Vha464I CTTAAG 1 cut(s) 614
VpaK11BI GGWCC 1 cut(s) 717
XapI RAATTY 1 cut(s) 570
XceI RCATGY 1 cut(s) 1011
XcmI CCANNNNNNNNNTGG 1 cut(s) 348
XspI CTAG 2 cut(s) 984, 1002
ZrmI AGTACT 1 cut(s) 1000
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.