Rw1G021410

Domain of unknown function (DUF313)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016389
Physical Location & Seq
Forward (+)
45649708 .. 45650739
1032 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G021410.1

Sequence Viewer

Length: 1032 bp
ATGGGTACACTGGTCAGTTCTTCAAATCTCTCATATCTTCGAGAACAAGAAGAAAAGATGGGATCTTTGAGTGCAGAAATTCACGAGGACAAGAAATTATGCGAGGACTGGTCTGCGCTTCATATGTTGGTAGAGGTTTCCTGCCATGTTAGCGAAGAAGAAAGAAAGGGTATGTGCGTGTTATCTTTGGTGAGTCAACAGGTATGCCCTAGGAAACAAAGGTCTCCACCCCGTGGTGAAAGTTCGAAAATGACGAGCCCTATCCCTAATATTGCTTCTGGTTCCTCTGATCAAGAAAATAGAAGAATTGATGATAAGGGTTTGAAAAGAAAAAGGAAGCCATGTGTTGATTGTCATAATGATAAAGACTTGGATGCCGCGGCGAAGAAGAAGAAGAAGAAGAGAACATATATGATGAAGGTGGACATGGGTGTTTATCAACCACCTCCTGATTTGCCCGAAAAGTACAAGCAAATGGTGAATGGGCCAAATGCGGCATTGGTGATACAAAAGCAGTTGTTTACGAGTGATGTGAACCCTGGCCTGAACCGGCTGACATTGCCACCAAAACAAATTTTGTGCAAGGAGTTGCTGAGACCTAATGAGATAGAAATCCTTAAGCCGAAGGATGCTTTGCTTAAAGTTCCATTTATAGACCCAGAGCTGAGAAAGGAAGAGATTTCACTGAGTTTATGGAAGCTGTCGAGCTCAAATGAAAGGTCCCTTGTGTTCAAATCTGAATGGAATCATGTTGTTAAGAAGAACGATCTGGGTGAAGGTGATCTGATCCAAATCTGGTCATTTCGGGATAGCAACGATCAACTTCAGTTGGCACTTGTTGTGGTGAAGAAAGCCGAGAAGTGCAAAAGTGGTGAGGAAGTGAGAAGCACCGCAAGAAGTAGTAGTGTGAGCAATGTCAGTGAGGACAGTGCAAGTGCAAGCCACAGTGGAAGAAAATGTCTCAAAATCCGATTTAAGTGTAATGCTAGCAGGAGTGAGAGTACTAGTACATGTGAATTATTGAGTCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF313

Protein Analysis

343

Amino Acids

38.62

Weight (kDa)

9.05

Isoelectric Point (pI)

59.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
At2g31720-like PF03754 157 - 254 1.1e-12 B3 domain-containing protein At2g31720-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016389)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g13550
prunus_persica Prupe.2G161700_v2.0.a1 Prupe.2G162000_v2.0.a1
rosa_laevigata RLG00000028289
rosa_multiflora Rmu_sc0001207.1_g000082 Rmu_sc0011028.1_g000001
rosa_roxburghii Rroxscaffold_4G00302050
rosa_rugosa Rorug01G0233600
rosa_samantha Rh1AG247500 Rh1BG217300 Rh1CG231000 Rh1DG243400
rosa_wichuraiana Rw0G018090 Rw1G021410 Rw1G021520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 233
AccII CGCG 1 cut(s) 380
AciI CCGC 4 cut(s) 378, 380, 494, 891
AclWI GGATC 2 cut(s) 70, 781
AcsI RAATTY 2 cut(s) 78, 573
AcuI CTGAAG 1 cut(s) 809
AdeI CACNNNGTG 1 cut(s) 233
AfaI GTAC 4 cut(s) 7, 467, 1003, 1009
AfiI CCNNNNNNNGG 1 cut(s) 233
AflII CTTAAG 1 cut(s) 617
AflIII ACRYGT 1 cut(s) 1010
AgsI TTSAA 3 cut(s) 24, 325, 733
AhlI ACTAGT 1 cut(s) 1004
AjnI CCWGG 1 cut(s) 538
AluBI AGCT 3 cut(s) 664, 700, 708
AluI AGCT 3 cut(s) 664, 700, 708
Alw21I GWGCWC 1 cut(s) 710
Alw26I GTCTC 3 cut(s) 228, 589, 965
AlwI GGATC 2 cut(s) 70, 781
AoxI GGCC 2 cut(s) 485, 541
ApoI RAATTY 2 cut(s) 78, 573
ArsI GACNNNNNNTTYG 2 cut(s) 704, 736
AspA2I CCTAGG 1 cut(s) 209
AspLEI GCGC 1 cut(s) 118
AspS9I GGNCC 2 cut(s) 485, 720
AsuHPI GGTGA 8 cut(s) 202, 248, 490, 514, 785, 791, 856, 884
AsuII TTCGAA 1 cut(s) 245
AsuNHI GCTAGC 1 cut(s) 986
AvaII GGWCC 1 cut(s) 720
AvrII CCTAGG 1 cut(s) 209
BaeI ACNNNNGTAYC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 260, 710
BauI CACGAG 1 cut(s) 83
Bbv12I GWGCWC 1 cut(s) 710
BccI CCATC 1 cut(s) 52
BciT130I CCWGG 1 cut(s) 540
BclI TGATCA 1 cut(s) 289
BcoDI GTCTC 3 cut(s) 228, 589, 965
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 3 cut(s) 210, 987, 1005
BfrI CTTAAG 1 cut(s) 617
BisI GCNGC 3 cut(s) 378, 381, 495
BlnI CCTAGG 1 cut(s) 209
BlsI GCNGC 3 cut(s) 379, 382, 496
BmcAI AGTACT 1 cut(s) 1003
Bme1390I CCNGG 1 cut(s) 540
Bme18I GGWCC 1 cut(s) 720
BmgT120I GGNCC 2 cut(s) 485, 720
BmiI GGNNCC 2 cut(s) 283, 722
BmrFI CCNGG 1 cut(s) 540
BmsI GCATC 2 cut(s) 364, 619
BmtI GCTAGC 1 cut(s) 990
Bpu14I TTCGAA 1 cut(s) 245
BsaBI GATNNNNATC 2 cut(s) 611, 791
BsaI GGTCTC 2 cut(s) 228, 589
BsaJI CCNNGG 4 cut(s) 209, 232, 378, 538
BsaXI ACNNNNNCTCC 2 cut(s) 985, 1015
Bsc4I CCNNNNNNNGG 1 cut(s) 233
Bse118I RCCGGY 1 cut(s) 549
Bse1I ACTGG 2 cut(s) 15, 113
Bse3DI GCAATG 2 cut(s) 557, 919
Bse8I GATNNNNATC 2 cut(s) 611, 791
BseBI CCWGG 1 cut(s) 540
BseDI CCNNGG 4 cut(s) 209, 232, 378, 538
BseGI GGATG 2 cut(s) 379, 634
BseJI GATNNNNATC 2 cut(s) 611, 791
BseLI CCNNNNNNNGG 1 cut(s) 233
BseMI GCAATG 2 cut(s) 557, 919
BseMII CTCAG 3 cut(s) 584, 656, 677
BseNI ACTGG 2 cut(s) 15, 113
BsgI GTGCAG 1 cut(s) 93
Bsh1236I CGCG 1 cut(s) 380
BshFI GGCC 2 cut(s) 487, 543
BsiHKAI GWGCWC 1 cut(s) 710
BsiSI CCGG 1 cut(s) 550
BslFI GGGAC 1 cut(s) 706
BslI CCNNNNNNNGG 1 cut(s) 233
BsmAI GTCTC 3 cut(s) 228, 589, 965
BsmFI GGGAC 1 cut(s) 706
BsnI GGCC 2 cut(s) 487, 543
Bso31I GGTCTC 2 cut(s) 228, 589
Bsp119I TTCGAA 1 cut(s) 245
Bsp1286I GDGCHC 2 cut(s) 260, 710
Bsp143I GATC 6 cut(s) 62, 289, 766, 781, 786, 817
BspACI CCGC 4 cut(s) 378, 380, 494, 891
BspANI GGCC 2 cut(s) 487, 543
BspCNI CTCAG 3 cut(s) 585, 657, 678
BspFNI CGCG 1 cut(s) 380
BspLI GGNNCC 2 cut(s) 283, 722
BspOI GCTAGC 1 cut(s) 990
BspPI GGATC 2 cut(s) 70, 781
BspT104I TTCGAA 1 cut(s) 245
BspTI CTTAAG 1 cut(s) 617
BspTNI GGTCTC 2 cut(s) 228, 589
BsrDI GCAATG 2 cut(s) 557, 919
BsrFI RCCGGY 1 cut(s) 549
BsrI ACTGG 2 cut(s) 15, 113
BssAI RCCGGY 1 cut(s) 549
BssECI CCNNGG 4 cut(s) 209, 232, 378, 538
BssMI GATC 6 cut(s) 62, 289, 766, 781, 786, 817
BssSI CACGAG 1 cut(s) 83
BssT1I CCWWGG 1 cut(s) 209
Bst2BI CACGAG 1 cut(s) 83
Bst2UI CCWGG 1 cut(s) 540
Bst4CI ACNGT 2 cut(s) 929, 947
Bst6I CTCTTC 2 cut(s) 395, 669
BstAFI CTTAAG 1 cut(s) 617
BstBI TTCGAA 1 cut(s) 245
BstC8I GCNNGC 2 cut(s) 940, 988
BstDEI CTNAG 3 cut(s) 593, 665, 686
BstDSI CCRYGG 2 cut(s) 232, 378
BstF5I GGATG 2 cut(s) 379, 634
BstFNI CGCG 1 cut(s) 380
BstHHI GCGC 1 cut(s) 118
BstKTI GATC 6 cut(s) 65, 292, 769, 784, 789, 820
BstMAI GTCTC 3 cut(s) 228, 589, 965
BstMBI GATC 6 cut(s) 62, 289, 766, 781, 786, 817
BstMWI GCNNNNNNNGC 2 cut(s) 150, 559
BstNI CCWGG 1 cut(s) 540
BstNSI RCATGY 1 cut(s) 1014
BstSCI CCNGG 1 cut(s) 538
BstUI CGCG 1 cut(s) 380
BstX2I RGATCY 1 cut(s) 62
BstYI RGATCY 1 cut(s) 62
BsuRI GGCC 2 cut(s) 487, 543
BtgI CCRYGG 2 cut(s) 232, 378
BtsCI GGATG 2 cut(s) 379, 634
BtsIMutI CAGTG 6 cut(s) 8, 683, 925, 934, 952, 1027
Cac8I GCNNGC 2 cut(s) 940, 988
CfoI GCGC 1 cut(s) 118
Cfr10I RCCGGY 1 cut(s) 549
Cfr13I GGNCC 2 cut(s) 485, 720
Cfr42I CCGCGG 1 cut(s) 381
Csp6I GTAC 4 cut(s) 6, 466, 1002, 1008
CviAII CATG 5 cut(s) 146, 342, 427, 749, 1011
CviQI GTAC 4 cut(s) 6, 466, 1002, 1008
DdeI CTNAG 3 cut(s) 593, 665, 686
DpnI GATC 6 cut(s) 64, 291, 768, 783, 788, 819
DpnII GATC 6 cut(s) 62, 289, 766, 781, 786, 817
DraIII CACNNNGTG 1 cut(s) 233
Eam1104I CTCTTC 2 cut(s) 395, 669
EarI CTCTTC 2 cut(s) 395, 669
Ecl136II GAGCTC 1 cut(s) 708
Eco130I CCWWGG 1 cut(s) 209
Eco24I GRGCYC 2 cut(s) 260, 710
Eco31I GGTCTC 2 cut(s) 228, 589
Eco47I GGWCC 1 cut(s) 720
Eco53kI GAGCTC 1 cut(s) 708
Eco57I CTGAAG 1 cut(s) 809
EcoICRI GAGCTC 1 cut(s) 708
EcoO109I RGGNCCY 1 cut(s) 720
EcoRII CCWGG 1 cut(s) 538
EcoT14I CCWWGG 1 cut(s) 209
EcoT38I GRGCYC 2 cut(s) 260, 710
ErhI CCWWGG 1 cut(s) 209
FaeI CATG 5 cut(s) 149, 345, 430, 752, 1014
FaqI GGGAC 1 cut(s) 706
FatI CATG 5 cut(s) 145, 341, 426, 748, 1010
FauNDI CATATG 1 cut(s) 123
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 3 cut(s) 378, 381, 495
FokI GGATG 2 cut(s) 386, 641
FriOI GRGCYC 2 cut(s) 260, 710
Fsp4HI GCNGC 3 cut(s) 378, 381, 495
FspBI CTAG 3 cut(s) 210, 987, 1005
GlaI GCGC 1 cut(s) 117
GluI GCNGC 3 cut(s) 378, 381, 495
HaeIII GGCC 2 cut(s) 487, 543
HapII CCGG 1 cut(s) 550
HhaI GCGC 1 cut(s) 118
Hin1II CATG 5 cut(s) 149, 345, 430, 752, 1014
Hin6I GCGC 1 cut(s) 116
HinP1I GCGC 1 cut(s) 116
HincII GTYRAC 1 cut(s) 197
HindII GTYRAC 1 cut(s) 197
HinfI GANTC 3 cut(s) 193, 745, 1024
HpaII CCGG 1 cut(s) 550
HphI GGTGA 8 cut(s) 202, 248, 490, 514, 785, 791, 856, 884
Hpy166II GTNNAC 5 cut(s) 8, 197, 424, 522, 535
Hpy188I TCNGA 4 cut(s) 289, 739, 786, 971
Hpy188III TCNNGA 5 cut(s) 41, 83, 293, 449, 806
Hpy8I GTNNAC 5 cut(s) 8, 197, 424, 522, 535
HpyAV CCTTC 3 cut(s) 412, 619, 770
HpyCH4III ACNGT 2 cut(s) 929, 947
HpyCH4V TGCA 5 cut(s) 74, 582, 864, 932, 938
HpyF10VI GCNNNNNNNGC 2 cut(s) 150, 559
HpyF3I CTNAG 3 cut(s) 593, 665, 686
Hsp92II CATG 5 cut(s) 149, 345, 430, 752, 1014
HspAI GCGC 1 cut(s) 116
Ksp22I TGATCA 1 cut(s) 289
KspI CCGCGG 1 cut(s) 381
Kzo9I GATC 6 cut(s) 62, 289, 766, 781, 786, 817
LweI GCATC 2 cut(s) 364, 619
MaeI CTAG 3 cut(s) 210, 987, 1005
MaeIII GTNAC 1 cut(s) 1025
MalI GATC 6 cut(s) 64, 291, 768, 783, 788, 819
MboI GATC 6 cut(s) 62, 289, 766, 781, 786, 817
MflI RGATCY 1 cut(s) 62
MhlI GDGCHC 2 cut(s) 260, 710
MluCI AATT 5 cut(s) 78, 95, 306, 573, 1016
MlyI GAGTC 1 cut(s) 202
MnlI CCTC 7 cut(s) 79, 97, 127, 295, 456, 868, 916
MseI TTAA 4 cut(s) 618, 639, 756, 975
MspA1I CMGCKG 1 cut(s) 380
MspCI CTTAAG 1 cut(s) 617
MspI CCGG 1 cut(s) 550
MspR9I CCNGG 1 cut(s) 540
MvaI CCWGG 1 cut(s) 540
MvnI CGCG 1 cut(s) 380
MwoI GCNNNNNNNGC 2 cut(s) 150, 559
NdeI CATATG 1 cut(s) 123
NdeII GATC 6 cut(s) 62, 289, 766, 781, 786, 817
NheI GCTAGC 1 cut(s) 986
NlaIII CATG 5 cut(s) 149, 345, 430, 752, 1014
NlaIV GGNNCC 2 cut(s) 283, 722
NmeAIII GCCGAG 1 cut(s) 880
NmuCI GTSAC 1 cut(s) 1025
NspI RCATGY 1 cut(s) 1014
NspV TTCGAA 1 cut(s) 245
PciI ACATGT 1 cut(s) 1010
PcsI WCGNNNNNNNCGW 1 cut(s) 251
PfeI GAWTC 1 cut(s) 745
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 3 cut(s) 379, 382, 496
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
PpuMI RGGWCCY 1 cut(s) 720
PscI ACATGT 1 cut(s) 1010
Psp124BI GAGCTC 1 cut(s) 710
Psp5II RGGWCCY 1 cut(s) 720
Psp6I CCWGG 1 cut(s) 538
PspGI CCWGG 1 cut(s) 538
PspN4I GGNNCC 2 cut(s) 283, 722
PspPI GGNCC 2 cut(s) 485, 720
PspPPI RGGWCCY 1 cut(s) 720
PsuI RGATCY 1 cut(s) 62
RsaI GTAC 4 cut(s) 7, 467, 1003, 1009
RsaNI GTAC 4 cut(s) 6, 466, 1002, 1008
SacI GAGCTC 1 cut(s) 710
SacII CCGCGG 1 cut(s) 381
SaqAI TTAA 4 cut(s) 618, 639, 756, 975
SatI GCNGC 3 cut(s) 378, 381, 495
Sau3AI GATC 6 cut(s) 62, 289, 766, 781, 786, 817
Sau96I GGNCC 2 cut(s) 485, 720
ScaI AGTACT 1 cut(s) 1003
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 540
SduI GDGCHC 2 cut(s) 260, 710
SfaNI GCATC 2 cut(s) 364, 619
Sfr303I CCGCGG 1 cut(s) 381
SfuI TTCGAA 1 cut(s) 245
SgrBI CCGCGG 1 cut(s) 381
SinI GGWCC 1 cut(s) 720
SmlI CTYRAG 1 cut(s) 617
SmoI CTYRAG 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 1004
Sse9I AATT 5 cut(s) 78, 95, 306, 573, 1016
SsiI CCGC 4 cut(s) 378, 380, 494, 891
SspI AATATT 1 cut(s) 271
SspMI CTAG 3 cut(s) 210, 987, 1005
SstI GAGCTC 1 cut(s) 710
StyD4I CCNGG 1 cut(s) 538
StyI CCWWGG 1 cut(s) 209
TaaI ACNGT 2 cut(s) 929, 947
TaqI TCGA 3 cut(s) 40, 245, 704
TasI AATT 5 cut(s) 78, 95, 306, 573, 1016
TatI WGTACW 3 cut(s) 465, 1001, 1007
TauI GCSGC 3 cut(s) 380, 383, 497
TfiI GAWTC 1 cut(s) 745
Tru1I TTAA 4 cut(s) 618, 639, 756, 975
Tru9I TTAA 4 cut(s) 618, 639, 756, 975
TscAI CASTG 5 cut(s) 15, 690, 925, 934, 952
TseFI GTSAC 1 cut(s) 1025
Tsp45I GTSAC 1 cut(s) 1025
TspDTI ATGAA 3 cut(s) 110, 431, 729
TspRI CASTG 5 cut(s) 15, 690, 925, 934, 952
Van91I CCANNNNNTGG 1 cut(s) 233
Vha464I CTTAAG 1 cut(s) 617
VpaK11BI GGWCC 1 cut(s) 720
XapI RAATTY 2 cut(s) 78, 573
XceI RCATGY 1 cut(s) 1014
XmaJI CCTAGG 1 cut(s) 209
XspI CTAG 3 cut(s) 210, 987, 1005
ZrmI AGTACT 1 cut(s) 1003
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.