Rmu_sc0001226.1_g000001

Heat shock factor protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001226.1
OG0012798
Physical Location & Seq
Forward (+)
68 .. 4280
4213 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1041 bp
atggccggtgctaatagcaacgccgacgaggcaccagccggcggtggggcccagccggctcaggctgcggcggggggaggtttggaatcggctccggcgccgacgccgttactgaactcgaacgcgccgccgccgttcctgagcaagacgtacgacatggtggacgacccggcgacggacccgattgtgtcgtggagcgccaccaacaacagcttcgtggtttggaaccctccggagttcgccagggaccttttgcccaagtacttcaagcacaataacttctccagtttcgtcaggcagttgaacacttatggatttaggaaagttgatcctgaccgctgggaatttgcaaatgagggctttttgaggggccaaaaacatcttctaaagagtatcaaccggcgaaaacctgcccatggacatgttcatcaacagccacagcagccatcacatggacagagttcagtagctgcatgtgtagaggttgggaagtttggtctcgaggaagagattgagaggcttaaaagggacaagaatgtgcttatgcaggaacttatcaggttgaggcagcagcaacagtctactgataatcagctgcaaacaatggtccaacgtcttcaaggtatggagcagcggcagcaacagatgatgtcattccttgcaaaggctgtgcagagtcccggtttcttgaatcagtttgtacagcagcaaaatgatagcaataggcgcttaactgaagtcaataagaaaaggagactcagggaggaaggaattgctgaaaacgagcgttctgatactcctgatggacagattgttaagtatcagcctccgatgaatgaagcggcaaaggcaatggtcaggcagatgatgaaaacaaatacttcttcccagctggattcttttagcaacaatcttgagaattttttgattggtgagggttcgtcatcatccagtgcagtagacagtggtagctcttcaagccatgtctcgggagctacacttcaagaggtcccgcctacttcagggcgtggaccatattaa

Protein Analysis

346

Amino Acids

38.28

Weight (kDa)

7.86

Isoelectric Point (pI)

59.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSF_DNA-bind PF00447 46 - 135 1.3e-30 HSF-type DNA-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012798)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G32330 AT1G32330
fragaria_vesca FvH4_5g22720
malus_domestica MD06G1037900.v1.1 MD16G1271200.v1.1
prunus_persica Prupe.5G031100_v2.0.a1
pyrus_communis pycom16g24070
rosa_chinensis RchiOBHm_Chr7g0211291
rosa_laevigata RLG00000003012
rosa_multiflora Rmu_co8434101.1_g000001 Rmu_sc0001226.1_g000001
rosa_roxburghii Rroxscaffold_3G00247950
rosa_rugosa Rorug07G0124900
rosa_samantha Rh7AG257200 Rh7BG251300 Rh7CG274000 Rh7DG264600
rosa_wichuraiana Rw7G021980

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 418
AccB1I GGYRCC 2 cut(s) 31, 97
AccB7I CCANNNNNTGG 1 cut(s) 452
AccI GTMKAC 2 cut(s) 581, 960
AccII CGCG 1 cut(s) 125
AccIII TCCGGA 1 cut(s) 232
AciI CCGC 9 cut(s) 42, 68, 71, 128, 131, 337, 634, 842, 1013
AclWI GGATC 1 cut(s) 323
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 344, 919
AcuI CTGAAG 2 cut(s) 756, 1005
AcyI GRCGYC 2 cut(s) 98, 104
AfaI GTAC 3 cut(s) 152, 263, 702
AfiI CCNNNNNNNGG 8 cut(s) 41, 45, 175, 416, 452, 664, 988, 1022
AflIII ACRYGT 1 cut(s) 421
AgsI TTSAA 6 cut(s) 268, 304, 620, 691, 978, 1004
AjnI CCWGG 1 cut(s) 242
AjuI GAANNNNNNNTTGG 4 cut(s) 197, 229, 366, 398
AluBI AGCT 6 cut(s) 213, 470, 595, 892, 972, 995
AluI AGCT 6 cut(s) 213, 470, 595, 892, 972, 995
Alw26I GTCTC 3 cut(s) 503, 748, 991
AlwI GGATC 1 cut(s) 323
AlwNI CAGNNNCTG 1 cut(s) 470
Ama87I CYCGRG 2 cut(s) 500, 988
Aor13HI TCCGGA 1 cut(s) 232
AoxI GGCC 3 cut(s) 3, 48, 370
ApaI GGGCCC 1 cut(s) 52
ApeKI GCWGC 9 cut(s) 65, 442, 470, 568, 571, 595, 631, 637, 706
ApoI RAATTY 2 cut(s) 344, 919
AspLEI GCGC 4 cut(s) 100, 127, 200, 729
AspS9I GGNCC 8 cut(s) 48, 49, 178, 247, 370, 607, 1009, 1031
AsuC2I CCSGG 2 cut(s) 170, 681
AsuHPI GGTGA 1 cut(s) 944
AvaI CYCGRG 2 cut(s) 500, 988
AvaII GGWCC 5 cut(s) 178, 247, 607, 1009, 1031
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 2 cut(s) 31, 97
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 1 cut(s) 608
BbvI GCAGC 9 cut(s) 52, 454, 457, 580, 582, 583, 643, 649, 718
BccI CCATC 2 cut(s) 454, 797
BceAI ACGGC 2 cut(s) 91, 118
BciT130I CCWGG 1 cut(s) 244
BcnI CCSGG 2 cut(s) 170, 681
BcoDI GTCTC 3 cut(s) 503, 748, 991
BfoI RGCGCY 3 cut(s) 101, 201, 730
BfuAI ACCTGC 1 cut(s) 418
BglI GCCNNNNNGGC 2 cut(s) 29, 56
BmcAI AGTACT 1 cut(s) 263
Bme1390I CCNGG 3 cut(s) 170, 244, 681
Bme18I GGWCC 5 cut(s) 178, 247, 607, 1009, 1031
BmeT110I CYCGRG 2 cut(s) 500, 988
BmgT120I GGNCC 8 cut(s) 48, 49, 178, 247, 370, 607, 1009, 1031
BmrFI CCNGG 3 cut(s) 170, 244, 681
BpiI GAAGAC 1 cut(s) 608
BpmI CTGGAG 1 cut(s) 268
Bpu10I CCTNAGC 2 cut(s) 60, 140
BpuEI CTTGAG 1 cut(s) 935
BpuMI CCSGG 2 cut(s) 170, 681
BsaHI GRCGYC 2 cut(s) 98, 104
BsaI GGTCTC 1 cut(s) 503
BsaJI CCNNGG 2 cut(s) 243, 415
BsaWI WCCGGW 1 cut(s) 232
Bsc4I CCNNNNNNNGG 8 cut(s) 41, 45, 175, 416, 452, 664, 988, 1022
Bse118I RCCGGY 4 cut(s) 5, 38, 55, 399
Bse1I ACTGG 2 cut(s) 285, 951
Bse3DI GCAATG 1 cut(s) 858
BseAI TCCGGA 1 cut(s) 232
BseBI CCWGG 1 cut(s) 244
BseDI CCNNGG 2 cut(s) 243, 415
BseGI GGATG 1 cut(s) 947
BseLI CCNNNNNNNGG 8 cut(s) 41, 45, 175, 416, 452, 664, 988, 1022
BseMI GCAATG 1 cut(s) 858
BseMII CTCAG 3 cut(s) 74, 131, 772
BseNI ACTGG 2 cut(s) 285, 951
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 9 cut(s) 52, 454, 457, 580, 582, 583, 643, 649, 718
BseYI CCCAGC 3 cut(s) 51, 339, 888
BsgI GTGCAG 2 cut(s) 692, 975
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 3 cut(s) 5, 50, 372
BshNI GGYRCC 2 cut(s) 31, 97
BsiHKCI CYCGRG 2 cut(s) 500, 988
BsiSI CCGG 8 cut(s) 6, 39, 56, 95, 170, 233, 400, 681
BsiWI CGTACG 1 cut(s) 150
BslFI GGGAC 4 cut(s) 260, 542, 663, 995
BslI CCNNNNNNNGG 8 cut(s) 41, 45, 175, 416, 452, 664, 988, 1022
BsmAI GTCTC 3 cut(s) 503, 748, 991
BsmFI GGGAC 4 cut(s) 260, 542, 663, 995
BsnI GGCC 3 cut(s) 5, 50, 372
Bso31I GGTCTC 1 cut(s) 503
BsoBI CYCGRG 2 cut(s) 500, 988
Bsp120I GGGCCC 1 cut(s) 48
Bsp1286I GDGCHC 1 cut(s) 52
Bsp13I TCCGGA 1 cut(s) 232
Bsp1407I TGTACA 1 cut(s) 700
Bsp143I GATC 1 cut(s) 328
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 9 cut(s) 42, 68, 71, 128, 131, 337, 634, 842, 1013
BspANI GGCC 3 cut(s) 5, 50, 372
BspCNI CTCAG 3 cut(s) 73, 132, 771
BspEI TCCGGA 1 cut(s) 232
BspFNI CGCG 1 cut(s) 125
BspMI ACCTGC 1 cut(s) 418
BspPI GGATC 1 cut(s) 323
BspQI GCTCTTC 1 cut(s) 979
BspT107I GGYRCC 2 cut(s) 31, 97
BspTNI GGTCTC 1 cut(s) 503
BsrDI GCAATG 1 cut(s) 858
BsrFI RCCGGY 4 cut(s) 5, 38, 55, 399
BsrGI TGTACA 1 cut(s) 700
BsrI ACTGG 2 cut(s) 285, 951
BssAI RCCGGY 4 cut(s) 5, 38, 55, 399
BssECI CCNNGG 2 cut(s) 243, 415
BssMI GATC 1 cut(s) 328
BssNI GRCGYC 2 cut(s) 98, 104
BssT1I CCWWGG 1 cut(s) 415
Bst2UI CCWGG 1 cut(s) 244
Bst4CI ACNGT 2 cut(s) 579, 965
Bst6I CTCTTC 2 cut(s) 501, 979
BstACI GRCGYC 2 cut(s) 98, 104
BstAUI TGTACA 1 cut(s) 700
BstC8I GCNNGC 2 cut(s) 40, 57
BstDEI CTNAG 3 cut(s) 60, 140, 758
BstDSI CCRYGG 1 cut(s) 415
BstENI CCTNNNNNAGG 2 cut(s) 662, 1020
BstF5I GGATG 1 cut(s) 947
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 3 cut(s) 101, 201, 730
BstHHI GCGC 4 cut(s) 100, 127, 200, 729
BstKTI GATC 1 cut(s) 331
BstMAI GTCTC 3 cut(s) 503, 748, 991
BstMBI GATC 1 cut(s) 328
BstMWI GCNNNNNNNGC 8 cut(s) 29, 56, 65, 442, 637, 726, 848, 978
BstNI CCWGG 1 cut(s) 244
BstNSI RCATGY 2 cut(s) 425, 477
BstSCI CCNGG 3 cut(s) 168, 242, 679
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 9 cut(s) 52, 454, 457, 580, 582, 583, 643, 649, 718
BstV2I GAAGAC 1 cut(s) 608
BsuRI GGCC 3 cut(s) 5, 50, 372
BtgI CCRYGG 1 cut(s) 415
BtsCI GGATG 1 cut(s) 947
BtsIMutI CAGTG 2 cut(s) 958, 970
BveI ACCTGC 1 cut(s) 418
Cac8I GCNNGC 2 cut(s) 40, 57
CaiI CAGNNNCTG 1 cut(s) 470
CfoI GCGC 4 cut(s) 100, 127, 200, 729
Cfr10I RCCGGY 4 cut(s) 5, 38, 55, 399
Cfr13I GGNCC 8 cut(s) 48, 49, 178, 247, 370, 607, 1009, 1031
CseI GACGC 1 cut(s) 112
Csp6I GTAC 3 cut(s) 151, 262, 701
CviAII CATG 6 cut(s) 157, 416, 422, 452, 474, 983
CviQI GTAC 3 cut(s) 151, 262, 701
DdeI CTNAG 3 cut(s) 60, 140, 758
DinI GGCGCC 1 cut(s) 99
DpnI GATC 1 cut(s) 330
DpnII GATC 1 cut(s) 328
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 501, 979
EarI CTCTTC 2 cut(s) 501, 979
Eco130I CCWWGG 1 cut(s) 415
Eco24I GRGCYC 1 cut(s) 52
Eco31I GGTCTC 1 cut(s) 503
Eco47I GGWCC 5 cut(s) 178, 247, 607, 1009, 1031
Eco57I CTGAAG 2 cut(s) 756, 1005
Eco88I CYCGRG 2 cut(s) 500, 988
EcoNI CCTNNNNNAGG 2 cut(s) 662, 1020
EcoO109I RGGNCCY 3 cut(s) 48, 247, 1009
EcoRII CCWGG 1 cut(s) 242
EcoT14I CCWWGG 1 cut(s) 415
EcoT38I GRGCYC 1 cut(s) 52
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 1 cut(s) 415
FaeI CATG 6 cut(s) 160, 419, 425, 455, 477, 986
FaqI GGGAC 4 cut(s) 260, 542, 663, 995
FatI CATG 6 cut(s) 156, 415, 421, 451, 473, 982
FauI CCCGC 2 cut(s) 64, 1020
FblI GTMKAC 2 cut(s) 581, 960
FokI GGATG 1 cut(s) 934
FriOI GRGCYC 1 cut(s) 52
GlaI GCGC 4 cut(s) 99, 126, 199, 728
GsaI CCCAGC 3 cut(s) 55, 343, 892
GsuI CTGGAG 1 cut(s) 268
HaeII RGCGCY 3 cut(s) 101, 201, 730
HaeIII GGCC 3 cut(s) 5, 50, 372
HapII CCGG 8 cut(s) 6, 39, 56, 95, 170, 233, 400, 681
HgaI GACGC 1 cut(s) 112
HhaI GCGC 4 cut(s) 100, 127, 200, 729
Hin1I GRCGYC 2 cut(s) 98, 104
Hin1II CATG 6 cut(s) 160, 419, 425, 455, 477, 986
Hin6I GCGC 4 cut(s) 98, 125, 198, 727
HinP1I GCGC 4 cut(s) 98, 125, 198, 727
HinfI GANTC 5 cut(s) 86, 676, 691, 756, 896
HpaII CCGG 8 cut(s) 6, 39, 56, 95, 170, 233, 400, 681
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 4 cut(s) 163, 582, 961, 1031
Hpy188I TCNGA 2 cut(s) 793, 831
Hpy188III TCNNGA 9 cut(s) 139, 233, 332, 500, 688, 800, 914, 990, 1004
Hpy8I GTNNAC 4 cut(s) 163, 582, 961, 1031
Hpy99I CGWCG 3 cut(s) 29, 106, 178
HpyAV CCTTC 1 cut(s) 761
HpyCH4III ACNGT 2 cut(s) 579, 965
HpyCH4IV ACGT 2 cut(s) 149, 613
HpyCH4V TGCA 7 cut(s) 350, 473, 547, 598, 662, 673, 956
HpyF10VI GCNNNNNNNGC 8 cut(s) 29, 56, 65, 442, 637, 726, 848, 978
HpyF3I CTNAG 3 cut(s) 60, 140, 758
HpySE526I ACGT 2 cut(s) 149, 613
Hsp92I GRCGYC 2 cut(s) 98, 104
Hsp92II CATG 6 cut(s) 160, 419, 425, 455, 477, 986
HspAI GCGC 4 cut(s) 98, 125, 198, 727
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 232
KroI GCCGGC 2 cut(s) 38, 55
KroNI GCCGGC 2 cut(s) 40, 57
Kzo9I GATC 1 cut(s) 328
LguI GCTCTTC 1 cut(s) 979
LmnI GCTCC 4 cut(s) 97, 195, 628, 992
Lsp1109I GCAGC 9 cut(s) 52, 454, 457, 580, 582, 583, 643, 649, 718
MaeII ACGT 2 cut(s) 149, 613
MaeIII GTNAC 1 cut(s) 108
MalI GATC 1 cut(s) 330
MboI GATC 1 cut(s) 328
MboII GAAGA 5 cut(s) 374, 518, 608, 876, 966
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 3 cut(s) 344, 771, 919
Mly113I GGCGCC 1 cut(s) 98
MlyI GAGTC 2 cut(s) 685, 750
MmeI TCCRAC 1 cut(s) 634
MroI TCCGGA 1 cut(s) 232
MroNI GCCGGC 2 cut(s) 38, 55
MseI TTAA 4 cut(s) 522, 731, 816, 1039
MslI CAYNNNNRTG 1 cut(s) 420
MspA1I CMGCKG 4 cut(s) 339, 595, 634, 892
MspI CCGG 8 cut(s) 6, 39, 56, 95, 170, 233, 400, 681
MspR9I CCNGG 3 cut(s) 170, 244, 681
MvaI CCWGG 1 cut(s) 244
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 8 cut(s) 29, 56, 65, 442, 637, 726, 848, 978
NaeI GCCGGC 2 cut(s) 40, 57
NarI GGCGCC 1 cut(s) 98
NciI CCSGG 2 cut(s) 170, 681
NcoI CCATGG 1 cut(s) 415
NdeII GATC 1 cut(s) 328
NgoMIV GCCGGC 2 cut(s) 38, 55
NlaIII CATG 6 cut(s) 160, 419, 425, 455, 477, 986
NspI RCATGY 2 cut(s) 425, 477
PaeR7I CTCGAG 1 cut(s) 500
PciI ACATGT 1 cut(s) 421
PciSI GCTCTTC 1 cut(s) 979
PdiI GCCGGC 2 cut(s) 40, 57
PfeI GAWTC 3 cut(s) 86, 691, 896
Pfl23II CGTACG 1 cut(s) 150
PflMI CCANNNNNTGG 1 cut(s) 452
PleI GAGTC 2 cut(s) 684, 750
PluTI GGCGCC 1 cut(s) 101
PpsI GAGTC 2 cut(s) 684, 750
PpuMI RGGWCCY 2 cut(s) 247, 1009
PscI ACATGT 1 cut(s) 421
Psp5II RGGWCCY 2 cut(s) 247, 1009
Psp6I CCWGG 1 cut(s) 242
PspFI CCCAGC 3 cut(s) 51, 339, 888
PspGI CCWGG 1 cut(s) 242
PspLI CGTACG 1 cut(s) 150
PspOMI GGGCCC 1 cut(s) 48
PspPI GGNCC 8 cut(s) 48, 49, 178, 247, 370, 607, 1009, 1031
PspPPI RGGWCCY 2 cut(s) 247, 1009
PstNI CAGNNNCTG 1 cut(s) 470
PvuII CAGCTG 2 cut(s) 595, 892
RsaI GTAC 3 cut(s) 152, 263, 702
RsaNI GTAC 3 cut(s) 151, 262, 701
RseI CAYNNNNRTG 1 cut(s) 420
SapI GCTCTTC 1 cut(s) 979
SaqAI TTAA 4 cut(s) 522, 731, 816, 1039
Sau3AI GATC 1 cut(s) 328
Sau96I GGNCC 8 cut(s) 48, 49, 178, 247, 370, 607, 1009, 1031
ScaI AGTACT 1 cut(s) 263
SchI GAGTC 2 cut(s) 685, 750
ScrFI CCNGG 3 cut(s) 170, 244, 681
SduI GDGCHC 1 cut(s) 52
SfoI GGCGCC 1 cut(s) 99
Sfr274I CTCGAG 1 cut(s) 500
SinI GGWCC 5 cut(s) 178, 247, 607, 1009, 1031
SlaI CTCGAG 1 cut(s) 500
SmiMI CAYNNNNRTG 1 cut(s) 420
SmlI CTYRAG 2 cut(s) 500, 914
SmoI CTYRAG 2 cut(s) 500, 914
Sse9I AATT 3 cut(s) 344, 771, 919
SsiI CCGC 9 cut(s) 42, 68, 71, 128, 131, 337, 634, 842, 1013
SspDI GGCGCC 1 cut(s) 97
StyD4I CCNGG 3 cut(s) 168, 242, 679
StyI CCWWGG 1 cut(s) 415
TaaI ACNGT 2 cut(s) 579, 965
TaiI ACGT 2 cut(s) 152, 616
TaqI TCGA 2 cut(s) 119, 501
TasI AATT 3 cut(s) 344, 771, 919
TatI WGTACW 2 cut(s) 261, 700
TauI GCSGC 5 cut(s) 71, 130, 133, 637, 845
TfiI GAWTC 3 cut(s) 86, 691, 896
Tru1I TTAA 4 cut(s) 522, 731, 816, 1039
Tru9I TTAA 4 cut(s) 522, 731, 816, 1039
TscAI CASTG 2 cut(s) 958, 970
TseI GCWGC 9 cut(s) 65, 442, 470, 568, 571, 595, 631, 637, 706
TspDTI ATGAA 4 cut(s) 416, 848, 852, 884
TspGWI ACGGA 1 cut(s) 191
TspRI CASTG 2 cut(s) 958, 970
Van91I CCANNNNNTGG 1 cut(s) 452
VpaK11BI GGWCC 5 cut(s) 178, 247, 607, 1009, 1031
XagI CCTNNNNNAGG 2 cut(s) 662, 1020
XapI RAATTY 2 cut(s) 344, 919
XceI RCATGY 2 cut(s) 425, 477
XhoI CTCGAG 1 cut(s) 500
XmiI GTMKAC 2 cut(s) 581, 960
ZrmI AGTACT 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.