Rmu_sc0001250.1_g000002

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001250.1
OG0007441
Physical Location & Seq
Reverse (-)
11607 .. 16705
5099 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1215 bp
atgggcatgggaacaagcacctttgttatcagatggatcaactttctcaccatgcttttagcaattgctgtggtagtcttcggagtatggatgagcactcatcatgatggctgtcggaaatccctcaccctccctgttttagccattggtgctgtcattttcttagtatctataattgggttcttgggcgcgctgaaaagaagttccatactgttatggatttatttgattctcctgtgcttcatattggtgggaatcctggtgttcacagtattagcgttcattgtcacaaataatggttctggtcatagcgtcgctgacctgaggttcaaggagtatcaacttcaggattacagttcatggtttctaaaacaattgaacaatacccataactggaagcacttaaagggttgtcttgtgaactcagatgattgcaataacctatcaaagaagtataagactctcaagaaattcaaattggcaaagctgacccctattgaagctggttgctgtcggccaccgtctgaatgcggttatcctgctgttaatgcctcatactatgacttgagctttcatccagttagttcaaacaaggactgcaaactttacagaaatgctcgggacaccaagtgctataattgtgattcctgcaaggctggagttgcacaatacatgaaaactgagtggagagtggttgcaattttcaatgttgctctatttgttgttttgtctctgatatactttgtgggctgttgtgcgagacgaaatgctgcaagagaccgtgatctcattaccgctagatttgcggattgtcactttgatgagacagtcttcccgtcgttagggagagataacaggaacgacatgaattatcgtggtctgtctccactatgtctcatctcgatccctgttaaagtgacgagatcacacatatctgctacaaatatgcctgcaaggaagaatgtccctacgagaggacgtagcgctaccctacacggaagtaggcatggcgccaacgcaaaagaaagtggcactctggctttacaagccatggccccagctaggatgcgtgagaggctcgtggattcgaaggatactttggcacattccgatcatttgatcatcaagactcaaaatcagtctcataagaatgttccgggttatggttattgttgggagacgcctcaacatcagaacctattcctgagaatatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Tetraspannin

Protein Analysis

404

Amino Acids

45.41

Weight (kDa)

9.53

Isoelectric Point (pI)

39.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 8 - 253 1.4e-34 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1010
AccII CGCG 1 cut(s) 191
AciI CCGC 3 cut(s) 531, 795, 806
AclWI GGATC 2 cut(s) 44, 898
AcoI YGGCCR 1 cut(s) 515
AcsI RAATTY 1 cut(s) 470
AcuI CTGAAG 1 cut(s) 329
AcyI GRCGYC 2 cut(s) 1011, 1181
AdeI CACNNNGTG 1 cut(s) 630
AfeI AGCGCT 1 cut(s) 985
AfiI CCNNNNNNNGG 5 cut(s) 393, 842, 974, 1062, 1163
AgsI TTSAA 6 cut(s) 331, 379, 475, 500, 588, 706
AjnI CCWGG 1 cut(s) 258
AjuI GAANNNNNNNTTGG 4 cut(s) 461, 493, 1082, 1114
AluBI AGCT 4 cut(s) 487, 503, 570, 1061
AluI AGCT 4 cut(s) 487, 503, 570, 1061
Alw21I GWGCWC 1 cut(s) 98
Alw26I GTCTC 8 cut(s) 735, 754, 771, 818, 888, 899, 1146, 1172
AlwI GGATC 2 cut(s) 44, 898
Ama87I CYCGRG 1 cut(s) 618
Aor51HI AGCGCT 1 cut(s) 985
AoxI GGCC 2 cut(s) 515, 1053
ApeKI GCWGC 1 cut(s) 770
ApoI RAATTY 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 1199
AspLEI GCGC 4 cut(s) 191, 193, 986, 1013
AspS9I GGNCC 1 cut(s) 1054
AsuC2I CCSGG 1 cut(s) 1158
AsuHPI GGTGA 2 cut(s) 40, 118
AsuII TTCGAA 1 cut(s) 1088
AvaI CYCGRG 1 cut(s) 618
AxyI CCTNAGG 1 cut(s) 323
BanI GGYRCC 1 cut(s) 1010
BauI CACGAG 1 cut(s) 1079
BbsI GAAGAC 2 cut(s) 70, 823
Bbv12I GWGCWC 1 cut(s) 98
BbvI GCAGC 1 cut(s) 757
BccI CCATC 2 cut(s) 27, 101
BciT130I CCWGG 1 cut(s) 260
BciVI GTATCC 1 cut(s) 1087
BclI TGATCA 1 cut(s) 1119
BcnI CCSGG 1 cut(s) 1158
BcoDI GTCTC 8 cut(s) 735, 754, 771, 818, 888, 899, 1146, 1172
BfaI CTAG 2 cut(s) 798, 1062
BfoI RGCGCY 2 cut(s) 987, 1014
BfuI GTATCC 1 cut(s) 1087
BisI GCNGC 1 cut(s) 771
BlsI GCNGC 1 cut(s) 772
Bme1390I CCNGG 2 cut(s) 260, 1158
BmeT110I CYCGRG 1 cut(s) 618
BmgT120I GGNCC 1 cut(s) 1054
BmiI GGNNCC 2 cut(s) 1012, 1056
BmrFI CCNGG 2 cut(s) 260, 1158
BmsI GCATC 1 cut(s) 1056
BpiI GAAGAC 2 cut(s) 70, 823
BpmI CTGGAG 1 cut(s) 678
Bpu14I TTCGAA 1 cut(s) 1088
BpuEI CTTGAG 2 cut(s) 449, 586
BpuMI CCSGG 1 cut(s) 1158
BsaHI GRCGYC 2 cut(s) 1011, 1181
BsaI GGTCTC 1 cut(s) 771
BsaJI CCNNGG 1 cut(s) 1050
Bsc4I CCNNNNNNNGG 5 cut(s) 393, 842, 974, 1062, 1163
Bse1I ACTGG 2 cut(s) 398, 578
Bse21I CCTNAGG 1 cut(s) 323
BseBI CCWGG 1 cut(s) 260
BseDI CCNNGG 1 cut(s) 1050
BseGI GGATG 3 cut(s) 96, 574, 1071
BseLI CCNNNNNNNGG 5 cut(s) 393, 842, 974, 1062, 1163
BseMII CTCAG 4 cut(s) 314, 438, 672, 1196
BseNI ACTGG 2 cut(s) 398, 578
BsePI GCGCGC 1 cut(s) 189
BseXI GCAGC 1 cut(s) 757
BseYI CCCAGC 1 cut(s) 1057
Bsh1236I CGCG 1 cut(s) 191
BshFI GGCC 2 cut(s) 517, 1055
BshNI GGYRCC 1 cut(s) 1010
BsiHKAI GWGCWC 1 cut(s) 98
BsiHKCI CYCGRG 1 cut(s) 618
BsiSI CCGG 1 cut(s) 1157
BslFI GGGAC 2 cut(s) 635, 950
BslI CCNNNNNNNGG 5 cut(s) 393, 842, 974, 1062, 1163
BsmAI GTCTC 8 cut(s) 735, 754, 771, 818, 888, 899, 1146, 1172
BsmBI CGTCTC 2 cut(s) 754, 1172
BsmFI GGGAC 2 cut(s) 635, 950
BsmI GAATGC 1 cut(s) 533
BsnI GGCC 2 cut(s) 517, 1055
Bso31I GGTCTC 1 cut(s) 771
BsoBI CYCGRG 1 cut(s) 618
Bsp119I TTCGAA 1 cut(s) 1088
Bsp1286I GDGCHC 1 cut(s) 98
Bsp143I GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
Bsp19I CCATGG 1 cut(s) 1050
BspACI CCGC 3 cut(s) 531, 795, 806
BspANI GGCC 2 cut(s) 517, 1055
BspCNI CTCAG 4 cut(s) 315, 437, 673, 1197
BspFNI CGCG 1 cut(s) 191
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 1012, 1056
BspPI GGATC 2 cut(s) 44, 898
BspT104I TTCGAA 1 cut(s) 1088
BspT107I GGYRCC 1 cut(s) 1010
BspTNI GGTCTC 1 cut(s) 771
BsrI ACTGG 2 cut(s) 398, 578
BssECI CCNNGG 1 cut(s) 1050
BssHII GCGCGC 1 cut(s) 189
BssMI GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
BssNI GRCGYC 2 cut(s) 1011, 1181
BssSI CACGAG 1 cut(s) 1079
BssT1I CCWWGG 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 1079
Bst2UI CCWGG 1 cut(s) 260
Bst4CI ACNGT 6 cut(s) 213, 271, 356, 522, 782, 829
BstACI GRCGYC 2 cut(s) 1011, 1181
BstBI TTCGAA 1 cut(s) 1088
BstC8I GCNNGC 2 cut(s) 191, 951
BstDEI CTNAG 5 cut(s) 163, 323, 424, 681, 1205
BstDSI CCRYGG 1 cut(s) 1050
BstENI CCTNNNNNAGG 1 cut(s) 972
BstF5I GGATG 3 cut(s) 96, 574, 1071
BstFNI CGCG 1 cut(s) 191
BstH2I RGCGCY 2 cut(s) 987, 1014
BstHHI GCGC 4 cut(s) 191, 193, 986, 1013
BstKTI GATC 6 cut(s) 39, 787, 906, 926, 1114, 1122
BstMAI GTCTC 8 cut(s) 735, 754, 771, 818, 888, 899, 1146, 1172
BstMBI GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
BstMWI GCNNNNNNNGC 6 cut(s) 149, 548, 662, 803, 1046, 1075
BstNI CCWGG 1 cut(s) 260
BstSCI CCNGG 2 cut(s) 258, 1156
BstUI CGCG 1 cut(s) 191
BstV1I GCAGC 1 cut(s) 757
BstV2I GAAGAC 2 cut(s) 70, 823
Bsu36I CCTNAGG 1 cut(s) 323
BsuI GTATCC 1 cut(s) 1087
BsuRI GGCC 2 cut(s) 517, 1055
BtgI CCRYGG 1 cut(s) 1050
BtsCI GGATG 3 cut(s) 96, 574, 1071
Cac8I GCNNGC 2 cut(s) 191, 951
CciI TCATGA 1 cut(s) 103
CfoI GCGC 4 cut(s) 191, 193, 986, 1013
Cfr13I GGNCC 1 cut(s) 1054
CseI GACGC 2 cut(s) 301, 1189
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 8 cut(s) 7, 52, 104, 360, 673, 865, 1007, 1051
DdeI CTNAG 5 cut(s) 163, 323, 424, 681, 1205
DinI GGCGCC 1 cut(s) 1012
DpnI GATC 6 cut(s) 38, 786, 905, 925, 1113, 1121
DpnII GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
DraIII CACNNNGTG 1 cut(s) 630
EaeI YGGCCR 1 cut(s) 515
Eco130I CCWWGG 1 cut(s) 1050
Eco31I GGTCTC 1 cut(s) 771
Eco47III AGCGCT 1 cut(s) 985
Eco57I CTGAAG 1 cut(s) 329
Eco81I CCTNAGG 1 cut(s) 323
Eco88I CYCGRG 1 cut(s) 618
EcoNI CCTNNNNNAGG 1 cut(s) 972
EcoRII CCWGG 1 cut(s) 258
EcoT14I CCWWGG 1 cut(s) 1050
EgeI GGCGCC 1 cut(s) 1012
EheI GGCGCC 1 cut(s) 1012
ErhI CCWWGG 1 cut(s) 1050
Esp3I CGTCTC 2 cut(s) 754, 1172
FaeI CATG 8 cut(s) 10, 55, 107, 363, 676, 868, 1010, 1054
FaqI GGGAC 2 cut(s) 635, 950
FatI CATG 8 cut(s) 6, 51, 103, 359, 672, 864, 1006, 1050
FbaI TGATCA 1 cut(s) 1119
Fnu4HI GCNGC 1 cut(s) 771
FokI GGATG 3 cut(s) 103, 561, 1078
Fsp4HI GCNGC 1 cut(s) 771
FspBI CTAG 2 cut(s) 798, 1062
GlaI GCGC 4 cut(s) 190, 192, 985, 1012
GluI GCNGC 1 cut(s) 771
GsaI CCCAGC 1 cut(s) 1061
GsuI CTGGAG 1 cut(s) 678
HaeII RGCGCY 2 cut(s) 987, 1014
HaeIII GGCC 2 cut(s) 517, 1055
HapII CCGG 1 cut(s) 1157
HgaI GACGC 2 cut(s) 301, 1189
HhaI GCGC 4 cut(s) 191, 193, 986, 1013
Hin1I GRCGYC 2 cut(s) 1011, 1181
Hin1II CATG 8 cut(s) 10, 55, 107, 363, 676, 868, 1010, 1054
Hin6I GCGC 4 cut(s) 189, 191, 984, 1011
HinP1I GCGC 4 cut(s) 189, 191, 984, 1011
HinfI GANTC 6 cut(s) 229, 255, 460, 644, 1085, 1129
HpaII CCGG 1 cut(s) 1157
HphI GGTGA 2 cut(s) 40, 118
Hpy166II GTNNAC 2 cut(s) 267, 421
Hpy188I TCNGA 8 cut(s) 32, 83, 117, 427, 526, 735, 1111, 1194
Hpy188III TCNNGA 7 cut(s) 104, 347, 466, 620, 901, 1126, 1204
Hpy8I GTNNAC 2 cut(s) 267, 421
Hpy99I CGWCG 2 cut(s) 317, 841
HpyAV CCTTC 1 cut(s) 1084
HpyCH4III ACNGT 6 cut(s) 213, 271, 356, 522, 782, 829
HpyCH4IV ACGT 1 cut(s) 979
HpyCH4V TGCA 7 cut(s) 435, 600, 651, 665, 698, 773, 953
HpyF10VI GCNNNNNNNGC 6 cut(s) 149, 548, 662, 803, 1046, 1075
HpyF3I CTNAG 5 cut(s) 163, 323, 424, 681, 1205
HpySE526I ACGT 1 cut(s) 979
Hsp92I GRCGYC 2 cut(s) 1011, 1181
Hsp92II CATG 8 cut(s) 10, 55, 107, 363, 676, 868, 1010, 1054
HspAI GCGC 4 cut(s) 189, 191, 984, 1011
KasI GGCGCC 1 cut(s) 1010
Ksp22I TGATCA 1 cut(s) 1119
Kzo9I GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
Lsp1109I GCAGC 1 cut(s) 757
LweI GCATC 1 cut(s) 1056
MaeI CTAG 2 cut(s) 798, 1062
MaeII ACGT 1 cut(s) 979
MaeIII GTNAC 3 cut(s) 286, 812, 916
MalI GATC 6 cut(s) 38, 786, 905, 925, 1113, 1121
MboI GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
MboII GAAGA 3 cut(s) 70, 823, 970
MfeI CAATTG 2 cut(s) 63, 374
MhlI GDGCHC 1 cut(s) 98
MluCI AATT 8 cut(s) 63, 174, 374, 470, 476, 637, 699, 868
Mly113I GGCGCC 1 cut(s) 1011
MlyI GAGTC 2 cut(s) 454, 1123
MmeI TCCRAC 1 cut(s) 95
MnlI CCTC 7 cut(s) 134, 140, 318, 562, 968, 1068, 1194
MroXI GAANNNNTTC 1 cut(s) 1199
MseI TTAA 3 cut(s) 404, 546, 912
MslI CAYNNNNRTG 4 cut(s) 105, 248, 819, 1149
MspI CCGG 1 cut(s) 1157
MspR9I CCNGG 2 cut(s) 260, 1158
MunI CAATTG 2 cut(s) 63, 374
Mva1269I GAATGC 1 cut(s) 533
MvaI CCWGG 1 cut(s) 260
MvnI CGCG 1 cut(s) 191
MwoI GCNNNNNNNGC 6 cut(s) 149, 548, 662, 803, 1046, 1075
NarI GGCGCC 1 cut(s) 1011
NciI CCSGG 1 cut(s) 1158
NcoI CCATGG 1 cut(s) 1050
NdeII GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
NlaIII CATG 8 cut(s) 10, 55, 107, 363, 676, 868, 1010, 1054
NlaIV GGNNCC 2 cut(s) 1012, 1056
NmuCI GTSAC 3 cut(s) 286, 812, 916
NspV TTCGAA 1 cut(s) 1088
PagI TCATGA 1 cut(s) 103
PauI GCGCGC 1 cut(s) 189
PctI GAATGC 1 cut(s) 533
PdmI GAANNNNTTC 1 cut(s) 1199
PfeI GAWTC 4 cut(s) 229, 255, 644, 1085
PkrI GCNGC 1 cut(s) 772
PleI GAGTC 2 cut(s) 454, 1123
PluTI GGCGCC 1 cut(s) 1014
PpsI GAGTC 2 cut(s) 454, 1123
Psp6I CCWGG 1 cut(s) 258
PspFI CCCAGC 1 cut(s) 1057
PspGI CCWGG 1 cut(s) 258
PspN4I GGNNCC 2 cut(s) 1012, 1056
PspPI GGNCC 1 cut(s) 1054
PteI GCGCGC 1 cut(s) 189
RseI CAYNNNNRTG 4 cut(s) 105, 248, 819, 1149
SaqAI TTAA 3 cut(s) 404, 546, 912
SatI GCNGC 1 cut(s) 771
Sau3AI GATC 6 cut(s) 36, 784, 903, 923, 1111, 1119
Sau96I GGNCC 1 cut(s) 1054
SchI GAGTC 2 cut(s) 454, 1123
ScrFI CCNGG 2 cut(s) 260, 1158
SduI GDGCHC 1 cut(s) 98
SfaNI GCATC 1 cut(s) 1056
SfoI GGCGCC 1 cut(s) 1012
SfuI TTCGAA 1 cut(s) 1088
SmiMI CAYNNNNRTG 4 cut(s) 105, 248, 819, 1149
SmlI CTYRAG 2 cut(s) 464, 565
SmoI CTYRAG 2 cut(s) 464, 565
Sse9I AATT 8 cut(s) 63, 174, 374, 470, 476, 637, 699, 868
SsiI CCGC 3 cut(s) 531, 795, 806
SspDI GGCGCC 1 cut(s) 1010
SspMI CTAG 2 cut(s) 798, 1062
StyD4I CCNGG 2 cut(s) 258, 1156
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 6 cut(s) 213, 271, 356, 522, 782, 829
TaiI ACGT 1 cut(s) 982
TaqI TCGA 2 cut(s) 902, 1088
TasI AATT 8 cut(s) 63, 174, 374, 470, 476, 637, 699, 868
TfiI GAWTC 4 cut(s) 229, 255, 644, 1085
Tru1I TTAA 3 cut(s) 404, 546, 912
Tru9I TTAA 3 cut(s) 404, 546, 912
TseFI GTSAC 3 cut(s) 286, 812, 916
TseI GCWGC 1 cut(s) 770
Tsp45I GTSAC 3 cut(s) 286, 812, 916
TspDTI ATGAA 6 cut(s) 232, 271, 348, 563, 689, 881
TspGWI ACGGA 1 cut(s) 1011
XagI CCTNNNNNAGG 1 cut(s) 972
XapI RAATTY 1 cut(s) 470
XmnI GAANNNNTTC 1 cut(s) 1199
XspI CTAG 2 cut(s) 798, 1062
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.