Rmu_sc0001258.1_g000007

Protein ROOT PRIMORDIUM DEFECTIVE 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001258.1
OG0011284
Physical Location & Seq
Reverse (-)
33883 .. 35301
1419 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1419 bp
atgcatgattctttggtaagctatagtatagtcaagaatgctgctcatctactgcgtggggagaaagtcttcacttcaggttcgcaagacacagaatgtaatgacaggcaagaatgctgcttatctatttaccaagacagaaccatggttccgtacatcctattttgtaaggttggggttggcttcatccgtggaacacaagtatttaatttccagcacaagcgtactctggtgaatattagattgaaatgggttaaagatagggcactggatgctgttgtgactgctgagagggatctcaaagctgcctgtgttcttgtttccattatctcctcctcgtctgatggctgcctccccatataccatctctcttgtcatcgtggacaactcgatcttccttatgacctcaaggtctctacttttatcaggaggtacccatctgtttttctagagtcccatgtgctcgatagcggtggcactcgtgtcccatgttttagcttagctcctgaagccataaaactccaccaggaagagtccaatgtcgttcagcagaataagattgatatccttaataggcttcggaaactgctcatgttcactaggggctggaagcttcctttacaaaccattgaccagctgaaatgggacctaggtttgccatatgattaccagcattcagtaattccacatcaccctgatctattctctttcatcagcttacctgatgaccgtgttggcttaaagctcttatcctgggatgatgatcttgctgtctcccagttgcaaaagaattctgctatacaacaagaggaacaagatgtgagaagtggctgtttagcttttccaattgggtttactaggggttttggattgaagagaaaatgcatggagtggttgaaggagtggcaaagcctcccttataattctccatactgtgatgcctcccatttagatcctcgtacagatgaatcggagaagaggattgttggagtcttccatgagcttcttcatctcactatgcaaaagaaaactgaacgtaagaatgtgagcaaccttcgtaaacctttggccctaccccagaagttcactaaagtgtttgagcgtcatccaggtattttctacatttcaaaaaagtgtgccactcaaactgtcattctgagggaagcctacgatcgtcaacaactcttgcagaaacaccctactgcagcactgagagaaaaatttgcgatgctgttgaggaaaggtttgctggataggagcaaaggtctatacaagaaaagtccaggcgatgtatttgaagtggatccatcaaaaaatatcgttgataatatcagtgattaccaatccagctttagagaggattcggagaatgatttgttttatgagtatgactcagatgaacaacatgacccttgctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

472

Amino Acids

54.43

Weight (kDa)

8.3

Isoelectric Point (pI)

49.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 85 - 415 1.8e-118 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 921
AasI GACNNNNNNGTC 1 cut(s) 410
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AciI CCGC 1 cut(s) 471
AclWI GGATC 4 cut(s) 303, 947, 1298, 1311
AcsI RAATTY 2 cut(s) 790, 1220
AcuI CTGAAG 2 cut(s) 60, 528
AfaI GTAC 4 cut(s) 155, 226, 434, 961
AgsI TTSAA 5 cut(s) 247, 874, 898, 1128, 1298
AjnI CCWGG 4 cut(s) 525, 752, 1108, 1282
AleI CACNNNNGTG 1 cut(s) 1091
AloI GAACNNNNNNTCC 2 cut(s) 133, 165
Alw21I GWGCWC 1 cut(s) 465
Alw26I GTCTC 2 cut(s) 418, 778
AlwI GGATC 4 cut(s) 303, 947, 1298, 1311
AoxI GGCC 1 cut(s) 1068
ApeKI GCWGC 5 cut(s) 41, 117, 305, 348, 1205
ApoI RAATTY 2 cut(s) 790, 1220
Asp700I GAANNNNTTC 1 cut(s) 68
Asp718I GGTACC 1 cut(s) 432
AspA2I CCTAGG 1 cut(s) 649
AspS9I GGNCC 2 cut(s) 646, 1069
AsuHPI GGTGA 2 cut(s) 244, 683
AvaII GGWCC 1 cut(s) 646
AvrII CCTAGG 1 cut(s) 649
BaeGI GKGCMC 1 cut(s) 268
BamHI GGATCC 1 cut(s) 1303
BanI GGYRCC 1 cut(s) 432
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 2 cut(s) 61, 985
Bbv12I GWGCWC 1 cut(s) 465
BbvI GCAGC 5 cut(s) 28, 104, 292, 335, 1217
BccI CCATC 4 cut(s) 338, 372, 445, 1315
BciT130I CCWGG 4 cut(s) 527, 754, 1110, 1284
BcoDI GTCTC 2 cut(s) 418, 778
BfaI CTAG 4 cut(s) 449, 600, 650, 858
BfmI CTRYAG 2 cut(s) 22, 1203
BisI GCNGC 5 cut(s) 42, 118, 306, 349, 1206
BlnI CCTAGG 1 cut(s) 649
BlpI GCTNAGC 1 cut(s) 499
BlsI GCNGC 5 cut(s) 43, 119, 307, 350, 1207
Bme1390I CCNGG 4 cut(s) 527, 754, 1110, 1284
Bme18I GGWCC 1 cut(s) 646
BmgT120I GGNCC 2 cut(s) 646, 1069
BmiI GGNNCC 4 cut(s) 150, 434, 647, 1305
BmrFI CCNGG 4 cut(s) 527, 754, 1110, 1284
BmrI ACTGGG 1 cut(s) 772
BmsI GCATC 3 cut(s) 262, 928, 1218
BmuI ACTGGG 1 cut(s) 772
BpiI GAAGAC 2 cut(s) 61, 985
BplI GAGNNNNNCTC 2 cut(s) 1376, 1408
Bpu1102I GCTNAGC 1 cut(s) 499
BpuEI CTTGAG 1 cut(s) 392
BsaBI GATNNNNATC 2 cut(s) 563, 762
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 4 cut(s) 144, 190, 649, 753
BsaXI ACNNNNNCTCC 2 cut(s) 1249, 1279
Bse1I ACTGG 2 cut(s) 273, 778
Bse8I GATNNNNATC 2 cut(s) 563, 762
BseBI CCWGG 4 cut(s) 527, 754, 1110, 1284
BseDI CCNNGG 4 cut(s) 144, 190, 649, 753
BseGI GGATG 5 cut(s) 156, 186, 277, 763, 1105
BseJI GATNNNNATC 2 cut(s) 563, 762
BseMII CTCAG 4 cut(s) 279, 1148, 1202, 1407
BseNI ACTGG 2 cut(s) 273, 778
BseRI GAGGAG 2 cut(s) 322, 325
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 5 cut(s) 28, 104, 292, 335, 1217
Bsh1285I CGRYCG 1 cut(s) 1174
BshFI GGCC 1 cut(s) 1070
BshNI GGYRCC 1 cut(s) 432
BsiEI CGRYCG 1 cut(s) 1174
BsiHKAI GWGCWC 1 cut(s) 465
BslFI GGGAC 3 cut(s) 439, 470, 659
BsmAI GTCTC 2 cut(s) 418, 778
BsmFI GGGAC 3 cut(s) 439, 470, 659
BsmI GAATGC 3 cut(s) 43, 119, 673
BsnI GGCC 1 cut(s) 1070
Bso31I GGTCTC 1 cut(s) 418
Bsp1286I GDGCHC 2 cut(s) 268, 465
Bsp143I GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
Bsp1720I GCTNAGC 1 cut(s) 499
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 1 cut(s) 471
BspANI GGCC 1 cut(s) 1070
BspCNI CTCAG 4 cut(s) 280, 1149, 1203, 1406
BspLI GGNNCC 4 cut(s) 150, 434, 647, 1305
BspMAI CTGCAG 1 cut(s) 1207
BspPI GGATC 4 cut(s) 303, 947, 1298, 1311
BspT107I GGYRCC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 418
BsrI ACTGG 2 cut(s) 273, 778
BssECI CCNNGG 4 cut(s) 144, 190, 649, 753
BssMI GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 2 cut(s) 144, 649
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 4 cut(s) 527, 754, 1110, 1284
Bst4CI ACNGT 3 cut(s) 731, 935, 1150
Bst6I CTCTTC 3 cut(s) 525, 869, 971
BstAPI GCANNNNNTGC 1 cut(s) 272
BstDEI CTNAG 5 cut(s) 288, 499, 1157, 1211, 1393
BstDSI CCRYGG 2 cut(s) 144, 190
BstF5I GGATG 5 cut(s) 156, 186, 277, 763, 1105
BstKTI GATC 7 cut(s) 298, 394, 700, 766, 955, 1174, 1306
BstMAI GTCTC 2 cut(s) 418, 778
BstMBI GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
BstMCI CGRYCG 1 cut(s) 1174
BstMWI GCNNNNNNNGC 2 cut(s) 272, 509
BstNI CCWGG 4 cut(s) 527, 754, 1110, 1284
BstSCI CCNGG 4 cut(s) 525, 752, 1108, 1282
BstSFI CTRYAG 2 cut(s) 22, 1203
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 5 cut(s) 28, 104, 292, 335, 1217
BstV2I GAAGAC 2 cut(s) 61, 985
BstX2I RGATCY 3 cut(s) 295, 952, 1303
BstYI RGATCY 3 cut(s) 295, 952, 1303
BsuRI GGCC 1 cut(s) 1070
BtgI CCRYGG 2 cut(s) 144, 190
BtgZI GCGATG 2 cut(s) 1241, 1302
BtsCI GGATG 5 cut(s) 156, 186, 277, 763, 1105
BtsIMutI CAGTG 3 cut(s) 266, 1208, 1339
Cfr13I GGNCC 2 cut(s) 646, 1069
CseI GACGC 1 cut(s) 1091
Csp6I GTAC 4 cut(s) 154, 225, 433, 960
CviAII CATG 8 cut(s) 5, 145, 458, 489, 592, 886, 998, 1406
CviQI GTAC 4 cut(s) 154, 225, 433, 960
DdeI CTNAG 5 cut(s) 288, 499, 1157, 1211, 1393
DpnI GATC 7 cut(s) 297, 393, 699, 765, 954, 1173, 1305
DpnII GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 3 cut(s) 525, 869, 971
EarI CTCTTC 3 cut(s) 525, 869, 971
Eco130I CCWWGG 2 cut(s) 144, 649
Eco31I GGTCTC 1 cut(s) 418
Eco32I GATATC 1 cut(s) 565
Eco47I GGWCC 1 cut(s) 646
Eco57I CTGAAG 2 cut(s) 60, 528
EcoO109I RGGNCCY 1 cut(s) 646
EcoRI GAATTC 1 cut(s) 790
EcoRII CCWGG 4 cut(s) 525, 752, 1108, 1282
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 2 cut(s) 144, 649
EcoT22I ATGCAT 2 cut(s) 6, 887
ErhI CCWWGG 2 cut(s) 144, 649
FaeI CATG 8 cut(s) 8, 148, 461, 492, 595, 889, 1001, 1409
FalI AAGNNNNNCTT 2 cut(s) 901, 933
FaqI GGGAC 3 cut(s) 439, 470, 659
FatI CATG 8 cut(s) 4, 144, 457, 488, 591, 885, 997, 1405
FauNDI CATATG 1 cut(s) 661
Fnu4HI GCNGC 5 cut(s) 42, 118, 306, 349, 1206
FokI GGATG 5 cut(s) 143, 173, 284, 770, 1092
Fsp4HI GCNGC 5 cut(s) 42, 118, 306, 349, 1206
FspBI CTAG 4 cut(s) 449, 600, 650, 858
GluI GCNGC 5 cut(s) 42, 118, 306, 349, 1206
HaeIII GGCC 1 cut(s) 1070
HgaI GACGC 1 cut(s) 1091
Hin1II CATG 8 cut(s) 8, 148, 461, 492, 595, 889, 1001, 1409
HincII GTYRAC 1 cut(s) 1178
HindII GTYRAC 1 cut(s) 1178
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 7 cut(s) 8, 452, 533, 968, 990, 1361, 1391
HphI GGTGA 2 cut(s) 244, 683
Hpy166II GTNNAC 6 cut(s) 383, 597, 855, 1061, 1086, 1178
Hpy188I TCNGA 6 cut(s) 343, 582, 973, 1158, 1366, 1396
Hpy188III TCNNGA 4 cut(s) 34, 427, 449, 506
Hpy8I GTNNAC 6 cut(s) 383, 597, 855, 1061, 1086, 1178
HpyAV CCTTC 2 cut(s) 892, 1064
HpyCH4III ACNGT 3 cut(s) 731, 935, 1150
HpyCH4IV ACGT 1 cut(s) 1036
HpyCH4V TGCA 6 cut(s) 4, 784, 885, 1021, 1189, 1205
HpyF10VI GCNNNNNNNGC 2 cut(s) 272, 509
HpyF3I CTNAG 5 cut(s) 288, 499, 1157, 1211, 1393
HpySE526I ACGT 1 cut(s) 1036
Hsp92II CATG 8 cut(s) 8, 148, 461, 492, 595, 889, 1001, 1409
KpnI GGTACC 1 cut(s) 436
Kzo9I GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
LmnI GCTCC 2 cut(s) 508, 1257
Lsp1109I GCAGC 5 cut(s) 28, 104, 292, 335, 1217
LweI GCATC 3 cut(s) 262, 928, 1218
MaeI CTAG 4 cut(s) 449, 600, 650, 858
MaeII ACGT 1 cut(s) 1036
MaeIII GTNAC 1 cut(s) 280
MalI GATC 7 cut(s) 297, 393, 699, 765, 954, 1173, 1305
MboI GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
MboII GAAGA 7 cut(s) 61, 386, 542, 886, 985, 988, 998
MfeI CAATTG 1 cut(s) 846
MflI RGATCY 3 cut(s) 295, 952, 1303
MhlI GDGCHC 2 cut(s) 268, 465
MluCI AATT 6 cut(s) 208, 681, 790, 846, 922, 1220
MlyI GAGTC 4 cut(s) 461, 542, 999, 1385
MmeI TCCRAC 1 cut(s) 967
Mph1103I ATGCAT 2 cut(s) 6, 887
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 4 cut(s) 207, 255, 570, 740
MslI CAYNNNNRTG 1 cut(s) 1091
MspA1I CMGCKG 1 cut(s) 637
MspR9I CCNGG 4 cut(s) 527, 754, 1110, 1284
MunI CAATTG 1 cut(s) 846
Mva1269I GAATGC 3 cut(s) 43, 119, 673
MvaI CCWGG 4 cut(s) 527, 754, 1110, 1284
MwoI GCNNNNNNNGC 2 cut(s) 272, 509
NcoI CCATGG 1 cut(s) 144
NdeI CATATG 1 cut(s) 661
NdeII GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
NlaIII CATG 8 cut(s) 8, 148, 461, 492, 595, 889, 1001, 1409
NlaIV GGNNCC 4 cut(s) 150, 434, 647, 1305
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 2 cut(s) 6, 887
OliI CACNNNNGTG 1 cut(s) 1091
PctI GAATGC 3 cut(s) 43, 119, 673
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 3 cut(s) 8, 968, 1361
PkrI GCNGC 5 cut(s) 43, 119, 307, 350, 1207
Ple19I CGATCG 1 cut(s) 1174
PleI GAGTC 4 cut(s) 460, 541, 998, 1385
PpsI GAGTC 4 cut(s) 460, 541, 998, 1385
PpuMI RGGWCCY 1 cut(s) 646
PsiI TTATAA 1 cut(s) 921
Psp5II RGGWCCY 1 cut(s) 646
Psp6I CCWGG 4 cut(s) 525, 752, 1108, 1282
PspGI CCWGG 4 cut(s) 525, 752, 1108, 1282
PspN4I GGNNCC 4 cut(s) 150, 434, 647, 1305
PspPI GGNCC 2 cut(s) 646, 1069
PspPPI RGGWCCY 1 cut(s) 646
PstI CTGCAG 1 cut(s) 1207
PsuI RGATCY 3 cut(s) 295, 952, 1303
PvuI CGATCG 1 cut(s) 1174
PvuII CAGCTG 1 cut(s) 637
RsaI GTAC 4 cut(s) 155, 226, 434, 961
RsaNI GTAC 4 cut(s) 154, 225, 433, 960
RseI CAYNNNNRTG 1 cut(s) 1091
SaqAI TTAA 4 cut(s) 207, 255, 570, 740
SatI GCNGC 5 cut(s) 42, 118, 306, 349, 1206
Sau3AI GATC 7 cut(s) 295, 391, 697, 763, 952, 1171, 1303
Sau96I GGNCC 2 cut(s) 646, 1069
SchI GAGTC 4 cut(s) 461, 542, 999, 1385
ScrFI CCNGG 4 cut(s) 527, 754, 1110, 1284
SduI GDGCHC 2 cut(s) 268, 465
SfaNI GCATC 3 cut(s) 262, 928, 1218
SfcI CTRYAG 2 cut(s) 22, 1203
SinI GGWCC 1 cut(s) 646
SmiMI CAYNNNNRTG 1 cut(s) 1091
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 6 cut(s) 208, 681, 790, 846, 922, 1220
SsiI CCGC 1 cut(s) 471
SspI AATATT 1 cut(s) 238
SspMI CTAG 4 cut(s) 449, 600, 650, 858
StyD4I CCNGG 4 cut(s) 525, 752, 1108, 1282
StyI CCWWGG 2 cut(s) 144, 649
TaaI ACNGT 3 cut(s) 731, 935, 1150
TaiI ACGT 1 cut(s) 1039
TaqI TCGA 2 cut(s) 390, 465
TasI AATT 6 cut(s) 208, 681, 790, 846, 922, 1220
TfiI GAWTC 3 cut(s) 8, 968, 1361
Tru1I TTAA 4 cut(s) 207, 255, 570, 740
Tru9I TTAA 4 cut(s) 207, 255, 570, 740
TscAI CASTG 3 cut(s) 273, 1215, 1339
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 5 cut(s) 41, 117, 305, 348, 1205
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 5 cut(s) 175, 700, 981, 998, 1413
TspGWI ACGGA 2 cut(s) 141, 179
TspRI CASTG 3 cut(s) 273, 1215, 1339
VpaK11BI GGWCC 1 cut(s) 646
XapI RAATTY 2 cut(s) 790, 1220
XbaI TCTAGA 1 cut(s) 448
XmaJI CCTAGG 1 cut(s) 649
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 4 cut(s) 449, 600, 650, 858
Zsp2I ATGCAT 2 cut(s) 6, 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.