Rmu_sc0001334.1_g000001

Catalyzes xyloglucan endohydrolysis (XEH) and or endotransglycosylation (XET). Cleaves and religates xyloglucan polymers, an essential constituent of the primary cell wall, and thereby participates in cell wall construction of growing tissues

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001334.1
OG0014989
Physical Location & Seq
Reverse (-)
2 .. 1577
1576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 653 bp
atggctcctccttttctctctttaattcttttattcctgataaaggttgcttcgagttcagctcagggtccaccctcacctggatactacccaagttccagagttaggtcaatagggtttgatcagggttacagaaacctttggggtcctgagcaccaaaacgaagaccagggcggtataagcatttggcttgacaaaagctcaggaagtgggttcaagtcagttcgttcatatggttctgggtactttggtgcagccatcaaggttcagaccggctacactgccggagtgattacatcattctatctgtcgaacaatgaagaacaccctggggatcatgacgagatcgatatcgagttcttgggaacgactcttgataagccttatgttctgcaaaccaatgtgtggactaaaggaagcggagatgggaacaaccttattgggagggagcacaggttccatctctggtttgatccaacacatgactttcacaactatgctatagtgtataaccccagtgagatcatattcctggtggacgatgtgccgataagaaggtacgcaaggaagagcgatgccacattcccattgaggccgatgtgggtgtacggatccatatgggatgcatcatcatgggccacagaggaaggaaa

Protein Analysis

217

Amino Acids

24.32

Weight (kDa)

5.42

Isoelectric Point (pI)

42.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_16 PF00722 41 - 217 1.8e-52 Glycosyl hydrolases family 16
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014989)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G44990
fragaria_vesca FvH4_3g30850
malus_domestica MD11G1146700.v1.1
prunus_persica Prupe.6G110300_v2.0.a1
pyrus_communis pycom11g11970
rosa_chinensis RchiOBHm_Chr5g0057091
rosa_laevigata RLG00000035150
rosa_multiflora Rmu_co8466263.1_g000001 Rmu_sc0001334.1_g000001 Rmu_sc0007667.1_g000003
rosa_roxburghii Rroxscaffold_1G00023110
rosa_rugosa Rorug05G0305700
rosa_samantha Rh5AG375000 Rh5BG386000 Rh5CG409500
rosa_wichuraiana Rw5G035300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 405
AciI CCGC 2 cut(s) 174, 420
AclWI GGATC 4 cut(s) 342, 467, 606, 619
AfaI GTAC 3 cut(s) 245, 560, 608
AfiI CCNNNNNNNGG 4 cut(s) 43, 80, 105, 405
AgsI TTSAA 1 cut(s) 217
AjnI CCWGG 4 cut(s) 79, 168, 328, 531
AloI GAACNNNNNNTCC 2 cut(s) 440, 472
AluBI AGCT 2 cut(s) 62, 201
AluI AGCT 2 cut(s) 62, 201
Alw21I GWGCWC 2 cut(s) 156, 453
AlwI GGATC 4 cut(s) 342, 467, 606, 619
AoxI GGCC 2 cut(s) 593, 636
ApeKI GCWGC 1 cut(s) 254
AspS9I GGNCC 3 cut(s) 68, 146, 636
AsuHPI GGTGA 1 cut(s) 69
AvaII GGWCC 2 cut(s) 68, 146
BaeI ACNNNNGTAYC 2 cut(s) 235, 268
BamHI GGATCC 1 cut(s) 611
BbsI GAAGAC 1 cut(s) 171
Bbv12I GWGCWC 2 cut(s) 156, 453
BbvI GCAGC 1 cut(s) 266
BccI CCATC 3 cut(s) 266, 419, 468
BciT130I CCWGG 4 cut(s) 81, 170, 330, 533
BciVI GTATCC 1 cut(s) 77
BclI TGATCA 1 cut(s) 121
BfmI CTRYAG 1 cut(s) 501
BfuI GTATCC 1 cut(s) 77
BisI GCNGC 1 cut(s) 255
BlsI GCNGC 1 cut(s) 256
Bme1390I CCNGG 4 cut(s) 81, 170, 330, 533
Bme18I GGWCC 2 cut(s) 68, 146
BmgT120I GGNCC 3 cut(s) 68, 146, 636
BmiI GGNNCC 5 cut(s) 6, 69, 147, 458, 613
BmrFI CCNGG 4 cut(s) 81, 170, 330, 533
BmrI ACTGGG 1 cut(s) 510
BmsI GCATC 3 cut(s) 565, 613, 635
BmuI ACTGGG 1 cut(s) 510
BpiI GAAGAC 1 cut(s) 171
BplI GAGNNNNNCTC 2 cut(s) 46, 78
Bpu10I CCTNAGC 3 cut(s) 63, 150, 202
Bsa29I ATCGAT 1 cut(s) 348
BsaBI GATNNNNATC 1 cut(s) 350
BsaJI CCNNGG 3 cut(s) 169, 328, 329
BsaXI ACNNNNNCTCC 2 cut(s) 440, 470
Bsc4I CCNNNNNNNGG 4 cut(s) 43, 80, 105, 405
Bse118I RCCGGY 1 cut(s) 272
Bse1I ACTGG 1 cut(s) 516
Bse8I GATNNNNATC 1 cut(s) 350
BseBI CCWGG 4 cut(s) 81, 170, 330, 533
BseCI ATCGAT 1 cut(s) 348
BseDI CCNNGG 3 cut(s) 169, 328, 329
BseGI GGATG 1 cut(s) 628
BseJI GATNNNNATC 1 cut(s) 350
BseLI CCNNNNNNNGG 4 cut(s) 43, 80, 105, 405
BseMII CTCAG 3 cut(s) 77, 141, 216
BseNI ACTGG 1 cut(s) 516
BseXI GCAGC 1 cut(s) 266
BsgI GTGCAG 1 cut(s) 273
BshFI GGCC 2 cut(s) 595, 638
BshVI ATCGAT 1 cut(s) 348
BsiHKAI GWGCWC 2 cut(s) 156, 453
BsiSI CCGG 2 cut(s) 273, 285
BslI CCNNNNNNNGG 4 cut(s) 43, 80, 105, 405
BsnI GGCC 2 cut(s) 595, 638
Bsp1286I GDGCHC 2 cut(s) 156, 453
Bsp143I GATC 6 cut(s) 121, 334, 345, 472, 522, 611
BspACI CCGC 2 cut(s) 174, 420
BspANI GGCC 2 cut(s) 595, 638
BspCNI CTCAG 3 cut(s) 76, 142, 215
BspDI ATCGAT 1 cut(s) 348
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 5 cut(s) 6, 69, 147, 458, 613
BspPI GGATC 4 cut(s) 342, 467, 606, 619
BspQI GCTCTTC 1 cut(s) 563
BsrFI RCCGGY 1 cut(s) 272
BsrI ACTGG 1 cut(s) 516
BssAI RCCGGY 1 cut(s) 272
BssECI CCNNGG 3 cut(s) 169, 328, 329
BssMI GATC 6 cut(s) 121, 334, 345, 472, 522, 611
Bst2UI CCWGG 4 cut(s) 81, 170, 330, 533
Bst6I CTCTTC 1 cut(s) 563
BstDEI CTNAG 3 cut(s) 63, 150, 202
BstENI CCTNNNNNAGG 1 cut(s) 41
BstF5I GGATG 1 cut(s) 628
BstKTI GATC 6 cut(s) 124, 337, 348, 475, 525, 614
BstMBI GATC 6 cut(s) 121, 334, 345, 472, 522, 611
BstMWI GCNNNNNNNGC 1 cut(s) 180
BstNI CCWGG 4 cut(s) 81, 170, 330, 533
BstSCI CCNGG 4 cut(s) 79, 168, 328, 531
BstSFI CTRYAG 1 cut(s) 501
BstV1I GCAGC 1 cut(s) 266
BstV2I GAAGAC 1 cut(s) 171
BstX2I RGATCY 1 cut(s) 611
BstYI RGATCY 1 cut(s) 611
Bsu15I ATCGAT 1 cut(s) 348
BsuI GTATCC 1 cut(s) 77
BsuRI GGCC 2 cut(s) 595, 638
BsuTUI ATCGAT 1 cut(s) 348
BtgZI GCGATG 1 cut(s) 588
BtsCI GGATG 1 cut(s) 628
BtsI GCAGTG 1 cut(s) 279
BtsIMutI CAGTG 2 cut(s) 279, 523
CciI TCATGA 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 272
Cfr13I GGNCC 3 cut(s) 68, 146, 636
ClaI ATCGAT 1 cut(s) 348
Csp6I GTAC 3 cut(s) 244, 559, 607
CviAII CATG 3 cut(s) 338, 482, 633
CviJI RGCY 9 cut(s) 5, 62, 190, 201, 257, 276, 382, 595, 638
CviKI_1 RGCY 9 cut(s) 5, 62, 190, 201, 257, 276, 382, 595, 638
CviQI GTAC 3 cut(s) 244, 559, 607
DdeI CTNAG 3 cut(s) 63, 150, 202
DpnI GATC 6 cut(s) 123, 336, 347, 474, 524, 613
DpnII GATC 6 cut(s) 121, 334, 345, 472, 522, 611
Eam1104I CTCTTC 1 cut(s) 563
EarI CTCTTC 1 cut(s) 563
Eco32I GATATC 1 cut(s) 352
Eco47I GGWCC 2 cut(s) 68, 146
EcoNI CCTNNNNNAGG 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 4 cut(s) 79, 168, 328, 531
EcoRV GATATC 1 cut(s) 352
EcoT22I ATGCAT 1 cut(s) 628
FaeI CATG 3 cut(s) 341, 485, 636
FatI CATG 3 cut(s) 337, 481, 632
FauNDI CATATG 2 cut(s) 232, 617
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 1 cut(s) 635
Fsp4HI GCNGC 1 cut(s) 255
GluI GCNGC 1 cut(s) 255
HaeIII GGCC 2 cut(s) 595, 638
HapII CCGG 2 cut(s) 273, 285
Hin1II CATG 3 cut(s) 341, 485, 636
HinfI GANTC 1 cut(s) 370
HpaII CCGG 2 cut(s) 273, 285
HphI GGTGA 1 cut(s) 69
Hpy166II GTNNAC 4 cut(s) 71, 408, 538, 607
Hpy188I TCNGA 1 cut(s) 270
Hpy188III TCNNGA 6 cut(s) 37, 99, 149, 204, 338, 374
Hpy8I GTNNAC 4 cut(s) 71, 408, 538, 607
HpyAV CCTTC 2 cut(s) 549, 641
HpyCH4V TGCA 3 cut(s) 254, 394, 626
HpyF10VI GCNNNNNNNGC 1 cut(s) 180
HpyF3I CTNAG 3 cut(s) 63, 150, 202
Hsp92II CATG 3 cut(s) 341, 485, 636
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 6 cut(s) 121, 334, 345, 472, 522, 611
LguI GCTCTTC 1 cut(s) 563
LmnI GCTCC 2 cut(s) 10, 448
Lsp1109I GCAGC 1 cut(s) 266
LweI GCATC 3 cut(s) 565, 613, 635
MaeIII GTNAC 1 cut(s) 128
MalI GATC 6 cut(s) 123, 336, 347, 474, 524, 613
MboI GATC 6 cut(s) 121, 334, 345, 472, 522, 611
MboII GAAGA 3 cut(s) 176, 332, 580
MflI RGATCY 1 cut(s) 611
MhlI GDGCHC 2 cut(s) 156, 453
MluCI AATT 1 cut(s) 24
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 500
MnlI CCTC 5 cut(s) 18, 85, 438, 585, 637
Mph1103I ATGCAT 1 cut(s) 628
MseI TTAA 1 cut(s) 23
MslI CAYNNNNRTG 2 cut(s) 495, 631
MspI CCGG 2 cut(s) 273, 285
MspR9I CCNGG 4 cut(s) 81, 170, 330, 533
MvaI CCWGG 4 cut(s) 81, 170, 330, 533
MwoI GCNNNNNNNGC 1 cut(s) 180
NdeI CATATG 2 cut(s) 232, 617
NdeII GATC 6 cut(s) 121, 334, 345, 472, 522, 611
NlaIII CATG 3 cut(s) 341, 485, 636
NlaIV GGNNCC 5 cut(s) 6, 69, 147, 458, 613
NsiI ATGCAT 1 cut(s) 628
PagI TCATGA 1 cut(s) 337
PasI CCCWGGG 1 cut(s) 329
PciSI GCTCTTC 1 cut(s) 563
PflMI CCANNNNNTGG 1 cut(s) 405
PkrI GCNGC 1 cut(s) 256
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 4 cut(s) 79, 168, 328, 531
PspGI CCWGG 4 cut(s) 79, 168, 328, 531
PspN4I GGNNCC 5 cut(s) 6, 69, 147, 458, 613
PspPI GGNCC 3 cut(s) 68, 146, 636
PspPPI RGGWCCY 1 cut(s) 146
PsuI RGATCY 1 cut(s) 611
RsaI GTAC 3 cut(s) 245, 560, 608
RsaNI GTAC 3 cut(s) 244, 559, 607
RseI CAYNNNNRTG 2 cut(s) 495, 631
SapI GCTCTTC 1 cut(s) 563
SaqAI TTAA 1 cut(s) 23
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 6 cut(s) 121, 334, 345, 472, 522, 611
Sau96I GGNCC 3 cut(s) 68, 146, 636
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 4 cut(s) 81, 170, 330, 533
SduI GDGCHC 2 cut(s) 156, 453
SfaNI GCATC 3 cut(s) 565, 613, 635
SfcI CTRYAG 1 cut(s) 501
SinI GGWCC 2 cut(s) 68, 146
SmiMI CAYNNNNRTG 2 cut(s) 495, 631
Sse9I AATT 1 cut(s) 24
SsiI CCGC 2 cut(s) 174, 420
StyD4I CCNGG 4 cut(s) 79, 168, 328, 531
TaqI TCGA 4 cut(s) 53, 311, 348, 354
TasI AATT 1 cut(s) 24
Tru1I TTAA 1 cut(s) 23
Tru9I TTAA 1 cut(s) 23
TscAI CASTG 2 cut(s) 286, 523
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 2 cut(s) 219, 333
TspGWI ACGGA 1 cut(s) 624
TspRI CASTG 2 cut(s) 286, 523
Van91I CCANNNNNTGG 1 cut(s) 405
VpaK11BI GGWCC 2 cut(s) 68, 146
XagI CCTNNNNNAGG 1 cut(s) 41
Zsp2I ATGCAT 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.