Rmu_sc0001702.1_g000002

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001702.1
OG0004865
Physical Location & Seq
Reverse (-)
5409 .. 7437
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1413 bp
atggaagacactacttgggttccctacgccactgcatggctgggagctctaaaccttatcctgttttcccgccatctccgccgccgaaaattcaatttacctccccgcccaaaaccttggcccatcattggaaacctcaacctcataggccacctccctcaccgttctgtccatgcactttcccaaatatatgggcccatcatgcagctgaaattcgggtccttccccattgtcgtggcctcctcggtcgaaatggccgaggccttcctcaaaactgaagacgttacttttgccgaccggcccaaaatggcggcgggaaagtacaccacctacaactactccaacatcacttggtccccttatggcccatattggagccagggccgtaagatgtgcatcatggaacttttcagcagcaaacgcctacagtcacatgaatacataagagaagaaatgagtgctttgcttagaggcttgtatgagtcctccaacactaacattttgctcaaagaccacttctccactgtgagtctcaatgtcataagcataatggtgcttggaaagaagtacacagacgagtccaaggatgcgatattgagccccgatgagtttaagaaattgcttgatgagctgttcttgctgagtggggtgttgaatattggtgatttcagtccttggcttgacttcttggacttgcaggggtatataaagcgaatgaaggctttaagcaagaagttggacaggttcttggagcatgtgttggatgagcatattgaaaataagaaggcaaaaggctttgttgcaacagatatggtggatgtgcttttgcagcttgctgatgatcccgaccttgtagttaagcttgaggggcatggggtcaagggctttacgcaggacctaattgcttgtgggacagagagctcagccgtgatagtggagtgggcaatttcggagcttctaagaaagccagagattttcaggaaggcaaccgaggagctagacagggtgattggaagggaaagatgggtggaagagagagacatttttcacttgccattcattgatgccattgccaaggaaaccatgaggcttcaccctgtggcacccatgttggtgccaagactagcacgtgaagattgccaagttgctggttataacattcctaagggcaacagagtcctagtgagtgtgtggacaataggaagagaccctaacttgtgggacaatcccaatgagttctgcccggaaaggttcattggcaaggacattgatgtcaagggccaagactttgagctgttgccatttgggtcaggaaggaggatgtgccctggttacagtcttggcattaacgttattcaatcaagtctggctaatatcttacatggttcacatggagattgcctgggaacatga

Protein Analysis

470

Amino Acids

53.41

Weight (kDa)

6.52

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 461 8.3e-86 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1155
AasI GACNNNNNNGTC 1 cut(s) 1271
AccB1I GGYRCC 2 cut(s) 1102, 1114
AccB7I CCANNNNNTGG 1 cut(s) 36
AciI CCGC 6 cut(s) 70, 79, 82, 106, 311, 314
AclI AACGTT 1 cut(s) 1350
AclWI GGATC 1 cut(s) 836
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 2 cut(s) 89, 212
AcuI CTGAAG 1 cut(s) 297
AcvI CACGTG 1 cut(s) 1130
AdeI CACNNNGTG 1 cut(s) 1099
AfaI GTAC 2 cut(s) 323, 569
AfiI CCNNNNNNNGG 2 cut(s) 36, 128
AgsI TTSAA 4 cut(s) 94, 655, 776, 1358
AjnI CCWGG 3 cut(s) 378, 1327, 1401
AjuI GAANNNNNNNTTGG 2 cut(s) 1239, 1271
AluBI AGCT 9 cut(s) 47, 208, 631, 832, 862, 921, 955, 997, 1294
AluI AGCT 9 cut(s) 47, 208, 631, 832, 862, 921, 955, 997, 1294
Alw21I GWGCWC 2 cut(s) 49, 923
Alw26I GTCTC 3 cut(s) 536, 1032, 1200
AlwI GGATC 1 cut(s) 836
ApaI GGGCCC 1 cut(s) 198
ApeKI GCWGC 3 cut(s) 205, 414, 829
ApoI RAATTY 2 cut(s) 89, 212
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AsuC2I CCSGG 1 cut(s) 1244
AsuHPI GGTGA 4 cut(s) 152, 674, 1018, 1085
AvaII GGWCC 3 cut(s) 219, 354, 895
AxyI CCTNAGG 1 cut(s) 1164
BaeGI GKGCMC 2 cut(s) 198, 1328
BanI GGYRCC 2 cut(s) 1102, 1114
BanII GRGCYC 4 cut(s) 49, 198, 602, 923
BbrPI CACGTG 1 cut(s) 1130
BbsI GAAGAC 2 cut(s) 12, 285
Bbv12I GWGCWC 2 cut(s) 49, 923
BbvI GCAGC 3 cut(s) 217, 426, 841
BccI CCATC 4 cut(s) 81, 131, 206, 1017
BceAI ACGGC 2 cut(s) 369, 911
BciT130I CCWGG 3 cut(s) 380, 1329, 1403
BcnI CCSGG 1 cut(s) 1244
BcoDI GTCTC 3 cut(s) 536, 1032, 1200
BfaI CTAG 3 cut(s) 998, 1124, 1181
BfmI CTRYAG 1 cut(s) 425
BisI GCNGC 5 cut(s) 82, 206, 312, 415, 830
BlpI GCTNAGC 1 cut(s) 922
BlsI GCNGC 5 cut(s) 83, 207, 313, 416, 831
Bme1390I CCNGG 4 cut(s) 380, 1244, 1329, 1403
Bme18I GGWCC 3 cut(s) 219, 354, 895
BmiI GGNNCC 7 cut(s) 21, 196, 220, 356, 377, 1104, 1116
BmrFI CCNGG 4 cut(s) 380, 1244, 1329, 1403
BmsI GCATC 3 cut(s) 405, 577, 1054
BpiI GAAGAC 2 cut(s) 12, 285
Bpu1102I GCTNAGC 1 cut(s) 922
BpuEI CTTGAG 1 cut(s) 884
BpuMI CCSGG 1 cut(s) 1244
BsaAI YACGTR 1 cut(s) 1130
BsaBI GATNNNNATC 1 cut(s) 395
BsaI GGTCTC 1 cut(s) 1200
BsaXI ACNNNNNCTCC 6 cut(s) 323, 353, 503, 533, 743, 773
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 128
Bse118I RCCGGY 1 cut(s) 297
Bse21I CCTNAGG 1 cut(s) 1164
Bse3DI GCAATG 1 cut(s) 1068
Bse8I GATNNNNATC 1 cut(s) 395
BseBI CCWGG 3 cut(s) 380, 1329, 1403
BseGI GGATG 4 cut(s) 592, 769, 823, 1326
BseJI GATNNNNATC 1 cut(s) 395
BseLI CCNNNNNNNGG 2 cut(s) 36, 128
BseMI GCAATG 1 cut(s) 1068
BseMII CTCAG 2 cut(s) 632, 936
BseRI GAGGAG 2 cut(s) 232, 1007
BseSI GKGCMC 2 cut(s) 198, 1328
BseXI GCAGC 3 cut(s) 217, 426, 841
BseYI CCCAGC 1 cut(s) 40
Bsh1285I CGRYCG 2 cut(s) 249, 298
BshNI GGYRCC 2 cut(s) 1102, 1114
BsiEI CGRYCG 2 cut(s) 249, 298
BsiHKAI GWGCWC 2 cut(s) 49, 923
BsiSI CCGG 2 cut(s) 298, 1244
BslFI GGGAC 3 cut(s) 340, 925, 1235
BslI CCNNNNNNNGG 2 cut(s) 36, 128
BsmAI GTCTC 3 cut(s) 536, 1032, 1200
BsmFI GGGAC 3 cut(s) 340, 925, 1235
Bso31I GGTCTC 1 cut(s) 1200
Bsp120I GGGCCC 1 cut(s) 194
Bsp1286I GDGCHC 5 cut(s) 49, 198, 602, 923, 1328
Bsp143I GATC 1 cut(s) 841
Bsp1720I GCTNAGC 1 cut(s) 922
BspACI CCGC 6 cut(s) 70, 79, 82, 106, 311, 314
BspCNI CTCAG 2 cut(s) 633, 935
BspLI GGNNCC 7 cut(s) 21, 196, 220, 356, 377, 1104, 1116
BspPI GGATC 1 cut(s) 836
BspT107I GGYRCC 2 cut(s) 1102, 1114
BspTNI GGTCTC 1 cut(s) 1200
BsrDI GCAATG 1 cut(s) 1068
BsrFI RCCGGY 1 cut(s) 297
BssAI RCCGGY 1 cut(s) 297
BssMI GATC 1 cut(s) 841
BssT1I CCWWGG 4 cut(s) 116, 582, 674, 1074
Bst2UI CCWGG 3 cut(s) 380, 1329, 1403
Bst4CI ACNGT 4 cut(s) 164, 429, 526, 1337
Bst6I CTCTTC 2 cut(s) 1026, 1198
BstBAI YACGTR 1 cut(s) 1130
BstC8I GCNNGC 1 cut(s) 834
BstDEI CTNAG 5 cut(s) 467, 641, 922, 959, 1164
BstF5I GGATG 4 cut(s) 592, 769, 823, 1326
BstKTI GATC 1 cut(s) 844
BstMAI GTCTC 3 cut(s) 536, 1032, 1200
BstMBI GATC 1 cut(s) 841
BstMCI CGRYCG 2 cut(s) 249, 298
BstMWI GCNNNNNNNGC 7 cut(s) 78, 202, 420, 628, 637, 829, 868
BstNI CCWGG 3 cut(s) 380, 1329, 1403
BstNSI RCATGY 1 cut(s) 758
BstSCI CCNGG 4 cut(s) 378, 1242, 1327, 1401
BstSFI CTRYAG 1 cut(s) 425
BstSLI GKGCMC 2 cut(s) 198, 1328
BstV1I GCAGC 3 cut(s) 217, 426, 841
BstV2I GAAGAC 2 cut(s) 12, 285
BstXI CCANNNNNNTGG 4 cut(s) 117, 191, 235, 1148
Bsu36I CCTNAGG 1 cut(s) 1164
BtsCI GGATG 4 cut(s) 592, 769, 823, 1326
BtsI GCAGTG 1 cut(s) 30
BtsIMutI CAGTG 2 cut(s) 30, 522
Cac8I GCNNGC 1 cut(s) 834
Cfr10I RCCGGY 1 cut(s) 297
Csp6I GTAC 2 cut(s) 322, 568
CviQI GTAC 2 cut(s) 322, 568
DdeI CTNAG 5 cut(s) 467, 641, 922, 959, 1164
DpnI GATC 1 cut(s) 843
DpnII GATC 1 cut(s) 841
DraIII CACNNNGTG 1 cut(s) 1099
DrdI GACNNNNNNGTC 1 cut(s) 1271
DseDI GACNNNNNNGTC 1 cut(s) 1271
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 2 cut(s) 1026, 1198
EarI CTCTTC 2 cut(s) 1026, 1198
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 2 cut(s) 47, 921
Eco130I CCWWGG 4 cut(s) 116, 582, 674, 1074
Eco147I AGGCCT 1 cut(s) 263
Eco24I GRGCYC 4 cut(s) 49, 198, 602, 923
Eco31I GGTCTC 1 cut(s) 1200
Eco47I GGWCC 3 cut(s) 219, 354, 895
Eco53kI GAGCTC 2 cut(s) 47, 921
Eco57I CTGAAG 1 cut(s) 297
Eco72I CACGTG 1 cut(s) 1130
Eco81I CCTNAGG 1 cut(s) 1164
EcoICRI GAGCTC 2 cut(s) 47, 921
EcoO109I RGGNCCY 2 cut(s) 219, 895
EcoRII CCWGG 3 cut(s) 378, 1327, 1401
EcoT14I CCWWGG 4 cut(s) 116, 582, 674, 1074
EcoT38I GRGCYC 4 cut(s) 49, 198, 602, 923
ErhI CCWWGG 4 cut(s) 116, 582, 674, 1074
FaqI GGGAC 3 cut(s) 340, 925, 1235
FauI CCCGC 3 cut(s) 77, 113, 307
Fnu4HI GCNGC 5 cut(s) 82, 206, 312, 415, 830
FokI GGATG 4 cut(s) 599, 776, 830, 1333
FriOI GRGCYC 4 cut(s) 49, 198, 602, 923
Fsp4HI GCNGC 5 cut(s) 82, 206, 312, 415, 830
FspBI CTAG 3 cut(s) 998, 1124, 1181
GluI GCNGC 5 cut(s) 82, 206, 312, 415, 830
GsaI CCCAGC 1 cut(s) 44
HapII CCGG 2 cut(s) 298, 1244
HindIII AAGCTT 1 cut(s) 860
HinfI GANTC 4 cut(s) 482, 529, 578, 1176
HpaII CCGG 2 cut(s) 298, 1244
HphI GGTGA 4 cut(s) 152, 674, 1018, 1085
Hpy166II GTNNAC 4 cut(s) 324, 570, 1194, 1388
Hpy188I TCNGA 1 cut(s) 952
Hpy188III TCNNGA 3 cut(s) 845, 979, 1311
Hpy8I GTNNAC 4 cut(s) 324, 570, 1194, 1388
HpyAV CCTTC 7 cut(s) 232, 274, 712, 778, 976, 1008, 1308
HpyCH4III ACNGT 4 cut(s) 164, 429, 526, 1337
HpyCH4IV ACGT 3 cut(s) 282, 1129, 1350
HpyCH4V TGCA 7 cut(s) 35, 176, 205, 396, 697, 803, 829
HpyF10VI GCNNNNNNNGC 7 cut(s) 78, 202, 420, 628, 637, 829, 868
HpyF3I CTNAG 5 cut(s) 467, 641, 922, 959, 1164
HpySE526I ACGT 3 cut(s) 282, 1129, 1350
Kzo9I GATC 1 cut(s) 841
LmnI GCTCC 5 cut(s) 44, 375, 751, 952, 994
Lsp1109I GCAGC 3 cut(s) 217, 426, 841
LweI GCATC 3 cut(s) 405, 577, 1054
MaeI CTAG 3 cut(s) 998, 1124, 1181
MaeII ACGT 3 cut(s) 282, 1129, 1350
MaeIII GTNAC 3 cut(s) 283, 429, 1331
MalI GATC 1 cut(s) 843
MboI GATC 1 cut(s) 841
MboII GAAGA 6 cut(s) 17, 290, 461, 1043, 1145, 1215
MhlI GDGCHC 5 cut(s) 49, 198, 602, 923, 1328
MluCI AATT 6 cut(s) 89, 94, 212, 617, 900, 945
MlyI GAGTC 4 cut(s) 491, 538, 587, 1185
MmeI TCCRAC 4 cut(s) 366, 513, 717, 741
MseI TTAA 4 cut(s) 612, 725, 858, 1347
MslI CAYNNNNRTG 3 cut(s) 233, 551, 1112
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 2 cut(s) 298, 1244
MspR9I CCNGG 4 cut(s) 380, 1244, 1329, 1403
MvaI CCWGG 3 cut(s) 380, 1329, 1403
MwoI GCNNNNNNNGC 7 cut(s) 78, 202, 420, 628, 637, 829, 868
NciI CCSGG 1 cut(s) 1244
NdeII GATC 1 cut(s) 841
NlaIV GGNNCC 7 cut(s) 21, 196, 220, 356, 377, 1104, 1116
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 429
NspI RCATGY 1 cut(s) 758
PceI AGGCCT 1 cut(s) 263
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PflMI CCANNNNNTGG 1 cut(s) 36
PkrI GCNGC 5 cut(s) 83, 207, 313, 416, 831
PleI GAGTC 4 cut(s) 490, 537, 586, 1184
PmaCI CACGTG 1 cut(s) 1130
PmlI CACGTG 1 cut(s) 1130
PpsI GAGTC 4 cut(s) 490, 537, 586, 1184
Ppu21I YACGTR 1 cut(s) 1130
PpuMI RGGWCCY 2 cut(s) 219, 895
PsiI TTATAA 1 cut(s) 1155
Psp124BI GAGCTC 2 cut(s) 49, 923
Psp1406I AACGTT 1 cut(s) 1350
Psp5II RGGWCCY 2 cut(s) 219, 895
Psp6I CCWGG 3 cut(s) 378, 1327, 1401
PspCI CACGTG 1 cut(s) 1130
PspFI CCCAGC 1 cut(s) 40
PspGI CCWGG 3 cut(s) 378, 1327, 1401
PspN4I GGNNCC 7 cut(s) 21, 196, 220, 356, 377, 1104, 1116
PspOMI GGGCCC 1 cut(s) 194
PspPPI RGGWCCY 2 cut(s) 219, 895
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 2 cut(s) 323, 569
RsaNI GTAC 2 cut(s) 322, 568
RseI CAYNNNNRTG 3 cut(s) 233, 551, 1112
SacI GAGCTC 2 cut(s) 49, 923
SaqAI TTAA 4 cut(s) 612, 725, 858, 1347
SatI GCNGC 5 cut(s) 82, 206, 312, 415, 830
Sau3AI GATC 1 cut(s) 841
SchI GAGTC 4 cut(s) 491, 538, 587, 1185
ScrFI CCNGG 4 cut(s) 380, 1244, 1329, 1403
SduI GDGCHC 5 cut(s) 49, 198, 602, 923, 1328
SfaNI GCATC 3 cut(s) 405, 577, 1054
SfcI CTRYAG 1 cut(s) 425
SinI GGWCC 3 cut(s) 219, 354, 895
SmiMI CAYNNNNRTG 3 cut(s) 233, 551, 1112
SmlI CTYRAG 1 cut(s) 863
SmoI CTYRAG 1 cut(s) 863
Sse9I AATT 6 cut(s) 89, 94, 212, 617, 900, 945
SseBI AGGCCT 1 cut(s) 263
SsiI CCGC 6 cut(s) 70, 79, 82, 106, 311, 314
SspI AATATT 1 cut(s) 658
SspMI CTAG 3 cut(s) 998, 1124, 1181
SstI GAGCTC 2 cut(s) 49, 923
StuI AGGCCT 1 cut(s) 263
StyD4I CCNGG 4 cut(s) 378, 1242, 1327, 1401
StyI CCWWGG 4 cut(s) 116, 582, 674, 1074
TaaI ACNGT 4 cut(s) 164, 429, 526, 1337
TaiI ACGT 3 cut(s) 285, 1132, 1353
TaqI TCGA 1 cut(s) 249
TaqII GACCGA 1 cut(s) 235
TasI AATT 6 cut(s) 89, 94, 212, 617, 900, 945
TatI WGTACW 2 cut(s) 321, 567
TauI GCSGC 2 cut(s) 84, 314
Tru1I TTAA 4 cut(s) 612, 725, 858, 1347
Tru9I TTAA 4 cut(s) 612, 725, 858, 1347
TscAI CASTG 2 cut(s) 37, 529
TseFI GTSAC 1 cut(s) 429
TseI GCWGC 3 cut(s) 205, 414, 829
Tsp45I GTSAC 1 cut(s) 429
TspDTI ATGAA 4 cut(s) 450, 731, 1048, 1243
TspRI CASTG 2 cut(s) 37, 529
Van91I CCANNNNNTGG 1 cut(s) 36
VpaK11BI GGWCC 3 cut(s) 219, 354, 895
XapI RAATTY 2 cut(s) 89, 212
XceI RCATGY 1 cut(s) 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 37
XspI CTAG 3 cut(s) 998, 1124, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.