Rw2G036590

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0004865
Physical Location & Seq
Reverse (-)
60220044 .. 60221881
1838 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G036590.1

Sequence Viewer

Length: 1527 bp
ATGGAAGGCACTGCTTGGGTTTCATATGCCGCGGCGTGGCTGGGAGTTCTAGCCCTCCTTCTCCTCGGCCTCCGCCTCCGCAGCCGCAAGCTCAATTTACCACCTGGACCAAAGCCCTGGCCAATCATTGGGAACCTCAACCTCATGGGCAATCTTCCCCATCGTTCACTCCACGAACTTTCCCAAAAGTACGGCCCCATCATGCAGCTCCGGTTCGGGTCCTTCCCCGTCGTCGTGGGCTCCTCAGCCGAAATGGCCGAAGCCTTCCTCAAAACCCATGATGTCGCAGTTGCTGACCGGCCCAAACTCGCTGCGGGCAAATATACCACTTACAACTACTCCAACATCACTTGGTCTCCTTACGGACCGTATTGGCGGCAAGCACGTAAAATGTGCATAATGGAGCTTTTCAGTGCTAAGCGCCTGGAGTCCTATGAATACATTAGAAAAGAAGAAATGAATGCATTGCTTAGAGGCCTATACAAGTCCTCCAACACCAACATCCTGTTGAAAGACCATCTTTCGGACTTGAGTCTAAATGTTATAAGCCGAATGGTGCTTGGAAAGAAGTACACAGACGAATCCGAAAACGCGATTGTGACTCCTGATGAGTTCAAGAAAATGCTTGATGAGCTGTTCTTGCTGACCGGCGTGTTGAACATTGGTGACTCAATACCTTGGCTTAATTTCTTGGATTTGCAAGGGTACATAAAGAGAATGAAGGCTTTGAGCAAGAAGTTCGATAGGTTTCTGGAGCATGTGTTGGATGAACATATTTCAAATAGCAAGGCACCTGACTTTGTTGCAAAAGATATGGTCGATGTGCTGTTGCAACTTGCTGCTGATCCTAACCTTGAAGTCAAGCTTGAAAGGCATGGGGTCAAGGCATTTAGCCAGGACCTGCTTGCTGGTGGAACAGAGAGCTCAGCCATGACCGTAGAATGGGGAATGTCGGAGGTCCTAAGGAAGCCAGAGATTTTCAACAAGGCGACAGAGGAGCTAGACAGGGTGATTGGAAGAGAGAGATGGGTTGAAGAGAAGGACATTGTCAACTTACCGTACATTGATGCCATTGCTAAAGAAACAATGAGGCTGCACCCTGTGGCACCCTTGTTGGTACCGAGACTAGCCCGTGAAGATTGCCAAGTTGCTGGGTATGACATTACTAAGGGCACCAGAGTCATAGTAAGCATATGGGCCATAGGAAGAGACCCTAAATATTGGGACAATCCCCTTGAGTTTTTCCCTGAGAGGTTTCTTGGAAAAGGTATCGATGTCAAAGGCCAAGACTTCGAGCTATTGCCTTTTGGGTCGGGCAGGAGGATGTGCCCTGGCTACAGTCTTGGCCACAAGCTGATTCAGTCAAGCATTGCAAATCTCTTGCATGGATTTAACTGGAGATTTCCTGATAATATGAATAAAGAGGATTTGAGTATGGAAGAAATTTTCGGGCTTTCAACACCAAGGAAGTTTCCCCTCATTGCAGTCACCGAGCCTCGTCTTCCAGCTCATCTTTACTCAATGTGA

Protein Analysis

508

Amino Acids

57.76

Weight (kDa)

8.17

Isoelectric Point (pI)

36.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 487 3.7e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 545
Acc36I ACCTGC 1 cut(s) 909
Acc65I GGTACC 1 cut(s) 1117
AccB1I GGYRCC 4 cut(s) 790, 1105, 1117, 1172
AccB7I CCANNNNNTGG 1 cut(s) 128
AccII CGCG 2 cut(s) 32, 593
AciI CCGC 7 cut(s) 30, 32, 73, 79, 85, 314, 376
AclWI GGATC 1 cut(s) 839
AcoI YGGCCR 3 cut(s) 119, 255, 1345
AcsI RAATTY 1 cut(s) 1443
AdeI CACNNNGTG 1 cut(s) 1102
AfaI GTAC 5 cut(s) 191, 572, 707, 1061, 1119
AfiI CCNNNNNNNGG 4 cut(s) 36, 128, 523, 942
AgsI TTSAA 9 cut(s) 511, 616, 658, 780, 857, 869, 982, 1034, 1458
AjnI CCWGG 5 cut(s) 103, 116, 423, 894, 1330
Alw21I GWGCWC 1 cut(s) 926
Alw26I GTCTC 3 cut(s) 360, 1117, 1203
AlwI GGATC 1 cut(s) 839
AlwNI CAGNNNCTG 2 cut(s) 293, 901
AoxI GGCC 9 cut(s) 67, 119, 193, 255, 299, 475, 1197, 1282, 1345
ApeKI GCWGC 5 cut(s) 81, 205, 311, 839, 1093
ApoI RAATTY 1 cut(s) 1443
Asp718I GGTACC 1 cut(s) 1117
AspLEI GCGC 1 cut(s) 423
AspS9I GGNCC 8 cut(s) 107, 194, 219, 300, 365, 898, 958, 1197
AsuHPI GGTGA 3 cut(s) 677, 1021, 1480
AvaII GGWCC 5 cut(s) 107, 219, 365, 898, 958
AxyI CCTNAGG 1 cut(s) 962
BaeGI GKGCMC 2 cut(s) 1175, 1331
BalI TGGCCA 2 cut(s) 121, 1347
BanI GGYRCC 4 cut(s) 790, 1105, 1117, 1172
BanII GRGCYC 2 cut(s) 242, 926
BbsI GAAGAC 1 cut(s) 1493
Bbv12I GWGCWC 1 cut(s) 926
BbvCI CCTCAGC 1 cut(s) 244
BbvI GCAGC 5 cut(s) 93, 217, 298, 826, 1080
BccI CCATC 4 cut(s) 168, 206, 525, 1020
BceAI ACGGC 1 cut(s) 208
BcgI CGANNNNNNTGC 2 cut(s) 721, 755
BciT130I CCWGG 5 cut(s) 105, 118, 425, 896, 1332
BcoDI GTCTC 3 cut(s) 360, 1117, 1203
BfaI CTAG 3 cut(s) 50, 1001, 1127
BfmI CTRYAG 1 cut(s) 1336
BfoI RGCGCY 1 cut(s) 424
BfuAI ACCTGC 1 cut(s) 909
BglI GCCNNNNNGGC 1 cut(s) 254
BisI GCNGC 9 cut(s) 30, 33, 82, 85, 206, 312, 377, 840, 1094
BlpI GCTNAGC 2 cut(s) 417, 925
BlsI GCNGC 9 cut(s) 31, 34, 83, 86, 207, 313, 378, 841, 1095
Bme1390I CCNGG 5 cut(s) 105, 118, 425, 896, 1332
Bme18I GGWCC 5 cut(s) 107, 219, 365, 898, 958
BmgT120I GGNCC 8 cut(s) 107, 194, 219, 300, 365, 898, 958, 1197
BmiI GGNNCC 8 cut(s) 134, 196, 220, 241, 792, 1107, 1119, 1174
BmrFI CCNGG 5 cut(s) 105, 118, 425, 896, 1332
BmsI GCATC 1 cut(s) 1057
BoxI GACNNNNGTC 1 cut(s) 531
BpiI GAAGAC 1 cut(s) 1493
BpmI CTGGAG 3 cut(s) 446, 773, 1417
Bpu10I CCTNAGC 1 cut(s) 244
Bpu1102I GCTNAGC 2 cut(s) 417, 925
BpuEI CTTGAG 2 cut(s) 550, 1256
Bsa29I ATCGAT 1 cut(s) 1272
BsaAI YACGTR 1 cut(s) 386
BsaI GGTCTC 2 cut(s) 360, 1203
BsaJI CCNNGG 6 cut(s) 30, 64, 116, 677, 1330, 1463
BsaWI WCCGGW 1 cut(s) 210
BsaXI ACNNNNNCTCC 8 cut(s) 323, 340, 353, 370, 473, 503, 746, 776
Bsc4I CCNNNNNNNGG 4 cut(s) 36, 128, 523, 942
Bse118I RCCGGY 2 cut(s) 297, 647
Bse1I ACTGG 1 cut(s) 1400
Bse21I CCTNAGG 1 cut(s) 962
Bse3DI GCAATG 4 cut(s) 464, 1071, 1368, 1479
BseBI CCWGG 5 cut(s) 105, 118, 425, 896, 1332
BseCI ATCGAT 1 cut(s) 1272
BseDI CCNNGG 6 cut(s) 30, 64, 116, 677, 1330, 1463
BseGI GGATG 3 cut(s) 501, 772, 1329
BseLI CCNNNNNNNGG 4 cut(s) 36, 128, 523, 942
BseMI GCAATG 4 cut(s) 464, 1071, 1368, 1479
BseMII CTCAG 3 cut(s) 258, 939, 1239
BseNI ACTGG 1 cut(s) 1400
BseRI GAGGAG 3 cut(s) 53, 232, 1010
BseSI GKGCMC 2 cut(s) 1175, 1331
BseXI GCAGC 5 cut(s) 93, 217, 298, 826, 1080
BseYI CCCAGC 2 cut(s) 40, 1151
BsgI GTGCAG 1 cut(s) 1079
Bsh1236I CGCG 2 cut(s) 32, 593
BshFI GGCC 9 cut(s) 69, 121, 195, 257, 301, 477, 1199, 1284, 1347
BshNI GGYRCC 4 cut(s) 790, 1105, 1117, 1172
BshVI ATCGAT 1 cut(s) 1272
BsiHKAI GWGCWC 1 cut(s) 926
BsiSI CCGG 3 cut(s) 211, 298, 648
BslFI GGGAC 1 cut(s) 1238
BslI CCNNNNNNNGG 4 cut(s) 36, 128, 523, 942
BsmAI GTCTC 3 cut(s) 360, 1117, 1203
BsmFI GGGAC 1 cut(s) 1238
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 9 cut(s) 69, 121, 195, 257, 301, 477, 1199, 1284, 1347
Bso31I GGTCTC 2 cut(s) 360, 1203
Bsp1286I GDGCHC 4 cut(s) 242, 926, 1175, 1331
Bsp143I GATC 1 cut(s) 844
Bsp1720I GCTNAGC 2 cut(s) 417, 925
BspACI CCGC 7 cut(s) 30, 32, 73, 79, 85, 314, 376
BspANI GGCC 9 cut(s) 69, 121, 195, 257, 301, 477, 1199, 1284, 1347
BspCNI CTCAG 3 cut(s) 257, 938, 1240
BspDI ATCGAT 1 cut(s) 1272
BspFNI CGCG 2 cut(s) 32, 593
BspLI GGNNCC 8 cut(s) 134, 196, 220, 241, 792, 1107, 1119, 1174
BspMI ACCTGC 1 cut(s) 909
BspPI GGATC 1 cut(s) 839
BspT107I GGYRCC 4 cut(s) 790, 1105, 1117, 1172
BspTNI GGTCTC 2 cut(s) 360, 1203
BsrDI GCAATG 4 cut(s) 464, 1071, 1368, 1479
BsrFI RCCGGY 2 cut(s) 297, 647
BsrI ACTGG 1 cut(s) 1400
BssAI RCCGGY 2 cut(s) 297, 647
BssECI CCNNGG 6 cut(s) 30, 64, 116, 677, 1330, 1463
BssMI GATC 1 cut(s) 844
BssT1I CCWWGG 2 cut(s) 677, 1463
Bst2UI CCWGG 5 cut(s) 105, 118, 425, 896, 1332
Bst4CI ACNGT 4 cut(s) 369, 937, 1059, 1340
Bst6I CTCTTC 3 cut(s) 1012, 1029, 1201
BstBAI YACGTR 1 cut(s) 386
BstC8I GCNNGC 4 cut(s) 89, 316, 381, 906
BstDEI CTNAG 7 cut(s) 244, 417, 470, 925, 962, 1167, 1248
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 501, 772, 1329
BstFNI CGCG 2 cut(s) 32, 593
BstH2I RGCGCY 1 cut(s) 424
BstHHI GCGC 1 cut(s) 423
BstKTI GATC 1 cut(s) 847
BstMAI GTCTC 3 cut(s) 360, 1117, 1203
BstMBI GATC 1 cut(s) 844
BstMWI GCNNNNNNNGC 5 cut(s) 81, 254, 631, 640, 871
BstNI CCWGG 5 cut(s) 105, 118, 425, 896, 1332
BstNSI RCATGY 1 cut(s) 761
BstPAI GACNNNNGTC 1 cut(s) 531
BstSCI CCNGG 5 cut(s) 103, 116, 423, 894, 1330
BstSFI CTRYAG 1 cut(s) 1336
BstSLI GKGCMC 2 cut(s) 1175, 1331
BstUI CGCG 2 cut(s) 32, 593
BstV1I GCAGC 5 cut(s) 93, 217, 298, 826, 1080
BstV2I GAAGAC 1 cut(s) 1493
BstXI CCANNNNNNTGG 2 cut(s) 117, 1151
Bsu15I ATCGAT 1 cut(s) 1272
Bsu36I CCTNAGG 1 cut(s) 962
BsuRI GGCC 9 cut(s) 69, 121, 195, 257, 301, 477, 1199, 1284, 1347
BsuTUI ATCGAT 1 cut(s) 1272
BtgI CCRYGG 1 cut(s) 30
BtsCI GGATG 3 cut(s) 501, 772, 1329
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 2 cut(s) 9, 418
BveI ACCTGC 1 cut(s) 909
Cac8I GCNNGC 4 cut(s) 89, 316, 381, 906
CaiI CAGNNNCTG 2 cut(s) 293, 901
CfoI GCGC 1 cut(s) 423
Cfr10I RCCGGY 2 cut(s) 297, 647
Cfr13I GGNCC 8 cut(s) 107, 194, 219, 300, 365, 898, 958, 1197
Cfr42I CCGCGG 1 cut(s) 33
ClaI ATCGAT 1 cut(s) 1272
CpoI CGGWCCG 1 cut(s) 365
Csp6I GTAC 5 cut(s) 190, 571, 706, 1060, 1118
CspI CGGWCCG 1 cut(s) 365
CviAII CATG 7 cut(s) 145, 202, 278, 758, 875, 931, 1385
CviQI GTAC 5 cut(s) 190, 571, 706, 1060, 1118
DdeI CTNAG 7 cut(s) 244, 417, 470, 925, 962, 1167, 1248
DpnI GATC 1 cut(s) 846
DpnII GATC 1 cut(s) 844
DraIII CACNNNGTG 1 cut(s) 1102
EaeI YGGCCR 3 cut(s) 119, 255, 1345
Eam1104I CTCTTC 3 cut(s) 1012, 1029, 1201
EarI CTCTTC 3 cut(s) 1012, 1029, 1201
EciI GGCGGA 1 cut(s) 62
Ecl136II GAGCTC 1 cut(s) 924
Eco130I CCWWGG 2 cut(s) 677, 1463
Eco147I AGGCCT 1 cut(s) 477
Eco24I GRGCYC 2 cut(s) 242, 926
Eco31I GGTCTC 2 cut(s) 360, 1203
Eco47I GGWCC 5 cut(s) 107, 219, 365, 898, 958
Eco53kI GAGCTC 1 cut(s) 924
Eco81I CCTNAGG 1 cut(s) 962
EcoICRI GAGCTC 1 cut(s) 924
EcoO109I RGGNCCY 3 cut(s) 219, 898, 958
EcoRII CCWGG 5 cut(s) 103, 116, 423, 894, 1330
EcoT14I CCWWGG 2 cut(s) 677, 1463
EcoT22I ATGCAT 1 cut(s) 466
EcoT38I GRGCYC 2 cut(s) 242, 926
ErhI CCWWGG 2 cut(s) 677, 1463
FaeI CATG 7 cut(s) 148, 205, 281, 761, 878, 934, 1388
FalI AAGNNNNNCTT 4 cut(s) 504, 536, 849, 881
FaqI GGGAC 1 cut(s) 1238
FatI CATG 7 cut(s) 144, 201, 277, 757, 874, 930, 1384
FauI CCCGC 1 cut(s) 307
FauNDI CATATG 2 cut(s) 25, 1193
Fnu4HI GCNGC 9 cut(s) 30, 33, 82, 85, 206, 312, 377, 840, 1094
FokI GGATG 3 cut(s) 488, 779, 1336
FriOI GRGCYC 2 cut(s) 242, 926
Fsp4HI GCNGC 9 cut(s) 30, 33, 82, 85, 206, 312, 377, 840, 1094
FspBI CTAG 3 cut(s) 50, 1001, 1127
GlaI GCGC 1 cut(s) 422
GluI GCNGC 9 cut(s) 30, 33, 82, 85, 206, 312, 377, 840, 1094
GsaI CCCAGC 2 cut(s) 44, 1155
GsuI CTGGAG 3 cut(s) 446, 773, 1417
HaeII RGCGCY 1 cut(s) 424
HaeIII GGCC 9 cut(s) 69, 121, 195, 257, 301, 477, 1199, 1284, 1347
HapII CCGG 3 cut(s) 211, 298, 648
HhaI GCGC 1 cut(s) 423
Hin1II CATG 7 cut(s) 148, 205, 281, 761, 878, 934, 1388
Hin6I GCGC 1 cut(s) 421
HinP1I GCGC 1 cut(s) 421
HincII GTYRAC 1 cut(s) 1051
HindII GTYRAC 1 cut(s) 1051
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 7 cut(s) 428, 532, 581, 601, 668, 1179, 1357
HpaII CCGG 3 cut(s) 211, 298, 648
HphI GGTGA 3 cut(s) 677, 1021, 1480
Hpy166II GTNNAC 3 cut(s) 167, 573, 1051
Hpy188I TCNGA 3 cut(s) 526, 586, 955
Hpy188III TCNNGA 4 cut(s) 605, 616, 752, 1406
Hpy8I GTNNAC 3 cut(s) 167, 573, 1051
Hpy99I CGWCG 2 cut(s) 233, 236
HpyAV CCTTC 5 cut(s) 68, 232, 274, 715, 1033
HpyCH4III ACNGT 4 cut(s) 369, 937, 1059, 1340
HpyCH4IV ACGT 1 cut(s) 385
HpyF10VI GCNNNNNNNGC 5 cut(s) 81, 254, 631, 640, 871
HpyF3I CTNAG 7 cut(s) 244, 417, 470, 925, 962, 1167, 1248
HpySE526I ACGT 1 cut(s) 385
Hsp92II CATG 7 cut(s) 148, 205, 281, 761, 878, 934, 1388
HspAI GCGC 1 cut(s) 421
KpnI GGTACC 1 cut(s) 1121
KspI CCGCGG 1 cut(s) 33
Kzo9I GATC 1 cut(s) 844
LmnI GCTCC 5 cut(s) 213, 245, 403, 754, 997
Lsp1109I GCAGC 5 cut(s) 93, 217, 298, 826, 1080
LweI GCATC 1 cut(s) 1057
MaeI CTAG 3 cut(s) 50, 1001, 1127
MaeII ACGT 1 cut(s) 385
MaeIII GTNAC 3 cut(s) 598, 665, 1486
MalI GATC 1 cut(s) 846
MboI GATC 1 cut(s) 844
MboII GAAGA 8 cut(s) 146, 464, 1029, 1046, 1148, 1218, 1451, 1493
MhlI GDGCHC 4 cut(s) 242, 926, 1175, 1331
MlsI TGGCCA 2 cut(s) 121, 1347
MluCI AATT 3 cut(s) 94, 685, 1443
MluNI TGGCCA 2 cut(s) 121, 1347
MlyI GAGTC 5 cut(s) 437, 541, 595, 662, 1188
MmeI TCCRAC 4 cut(s) 366, 516, 744, 933
Mox20I TGGCCA 2 cut(s) 121, 1347
Mph1103I ATGCAT 1 cut(s) 466
MscI TGGCCA 2 cut(s) 121, 1347
MseI TTAA 2 cut(s) 684, 1392
Msp20I TGGCCA 2 cut(s) 121, 1347
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 3 cut(s) 211, 298, 648
MspR9I CCNGG 5 cut(s) 105, 118, 425, 896, 1332
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 5 cut(s) 105, 118, 425, 896, 1332
MvnI CGCG 2 cut(s) 32, 593
MwoI GCNNNNNNNGC 5 cut(s) 81, 254, 631, 640, 871
NdeI CATATG 2 cut(s) 25, 1193
NdeII GATC 1 cut(s) 844
NlaIII CATG 7 cut(s) 148, 205, 281, 761, 878, 934, 1388
NlaIV GGNNCC 8 cut(s) 134, 196, 220, 241, 792, 1107, 1119, 1174
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 3 cut(s) 598, 665, 1486
NsiI ATGCAT 1 cut(s) 466
NspI RCATGY 1 cut(s) 761
PceI AGGCCT 1 cut(s) 477
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 581, 1357
PflFI GACNNNGTC 1 cut(s) 1046
PflMI CCANNNNNTGG 1 cut(s) 128
PkrI GCNGC 9 cut(s) 31, 34, 83, 86, 207, 313, 378, 841, 1095
PleI GAGTC 5 cut(s) 436, 540, 595, 662, 1187
PpsI GAGTC 5 cut(s) 436, 540, 595, 662, 1187
Ppu21I YACGTR 1 cut(s) 386
PpuMI RGGWCCY 3 cut(s) 219, 898, 958
PshAI GACNNNNGTC 1 cut(s) 531
PsiI TTATAA 1 cut(s) 545
Psp124BI GAGCTC 1 cut(s) 926
Psp5II RGGWCCY 3 cut(s) 219, 898, 958
Psp6I CCWGG 5 cut(s) 103, 116, 423, 894, 1330
PspFI CCCAGC 2 cut(s) 40, 1151
PspGI CCWGG 5 cut(s) 103, 116, 423, 894, 1330
PspN4I GGNNCC 8 cut(s) 134, 196, 220, 241, 792, 1107, 1119, 1174
PspPI GGNCC 8 cut(s) 107, 194, 219, 300, 365, 898, 958, 1197
PspPPI RGGWCCY 3 cut(s) 219, 898, 958
PstNI CAGNNNCTG 2 cut(s) 293, 901
PsyI GACNNNGTC 1 cut(s) 1046
RsaI GTAC 5 cut(s) 191, 572, 707, 1061, 1119
RsaNI GTAC 5 cut(s) 190, 571, 706, 1060, 1118
Rsr2I CGGWCCG 1 cut(s) 365
RsrII CGGWCCG 1 cut(s) 365
SacI GAGCTC 1 cut(s) 926
SacII CCGCGG 1 cut(s) 33
SaqAI TTAA 2 cut(s) 684, 1392
SatI GCNGC 9 cut(s) 30, 33, 82, 85, 206, 312, 377, 840, 1094
Sau3AI GATC 1 cut(s) 844
Sau96I GGNCC 8 cut(s) 107, 194, 219, 300, 365, 898, 958, 1197
SchI GAGTC 5 cut(s) 437, 541, 595, 662, 1188
ScrFI CCNGG 5 cut(s) 105, 118, 425, 896, 1332
SduI GDGCHC 4 cut(s) 242, 926, 1175, 1331
SfaNI GCATC 1 cut(s) 1057
SfcI CTRYAG 1 cut(s) 1336
Sfr303I CCGCGG 1 cut(s) 33
SgrBI CCGCGG 1 cut(s) 33
SinI GGWCC 5 cut(s) 107, 219, 365, 898, 958
SmlI CTYRAG 2 cut(s) 529, 1235
SmoI CTYRAG 2 cut(s) 529, 1235
Sse9I AATT 3 cut(s) 94, 685, 1443
SseBI AGGCCT 1 cut(s) 477
SsiI CCGC 7 cut(s) 30, 32, 73, 79, 85, 314, 376
SspI AATATT 1 cut(s) 1220
SspMI CTAG 3 cut(s) 50, 1001, 1127
SstI GAGCTC 1 cut(s) 926
StuI AGGCCT 1 cut(s) 477
StyD4I CCNGG 5 cut(s) 103, 116, 423, 894, 1330
StyI CCWWGG 2 cut(s) 677, 1463
TaaI ACNGT 4 cut(s) 369, 937, 1059, 1340
TaiI ACGT 1 cut(s) 388
TaqI TCGA 4 cut(s) 741, 819, 1272, 1293
TasI AATT 3 cut(s) 94, 685, 1443
TatI WGTACW 1 cut(s) 570
TauI GCSGC 4 cut(s) 32, 35, 87, 379
TfiI GAWTC 2 cut(s) 581, 1357
Tru1I TTAA 2 cut(s) 684, 1392
Tru9I TTAA 2 cut(s) 684, 1392
TscAI CASTG 2 cut(s) 16, 418
TseFI GTSAC 3 cut(s) 598, 665, 1486
TseI GCWGC 5 cut(s) 81, 205, 311, 839, 1093
Tsp45I GTSAC 3 cut(s) 598, 665, 1486
TspDTI ATGAA 6 cut(s) 12, 450, 473, 734, 783, 1430
TspGWI ACGGA 1 cut(s) 378
TspRI CASTG 2 cut(s) 16, 418
Tth111I GACNNNGTC 1 cut(s) 1046
Van91I CCANNNNNTGG 1 cut(s) 128
VpaK11BI GGWCC 5 cut(s) 107, 219, 365, 898, 958
XapI RAATTY 1 cut(s) 1443
XceI RCATGY 1 cut(s) 761
XspI CTAG 3 cut(s) 50, 1001, 1127
Zsp2I ATGCAT 1 cut(s) 466
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.