Rroxscaffold_152G00434780

Cytochrome p450

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000152
OG0004865
Physical Location & Seq
Reverse (-)
274107 .. 276322
2216 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_152G00434780.1

Sequence Viewer

Length: 1527 bp
ATGGAAGGCACTGCTTGGGTTTCCTATGCCGCCGCGTGGCTGGGAGCTCTAGCCCTTGTCCTGTTTTCCCGCCATCTCCGCCGCCGAAAACTCAATGTACCGCCCGGCCCTAAACCTTGGCCCATCATCGGAAACCTCAACCTCATAGGCCACCTCCCTCACCGTTCTGTCCATGCACTTTCCCAAATATATGGACCCATCATGCAGCTGAAATTCGGGTCCTTCCCCATTGTAGTGGCCTCCTCGGTCGAAATGGCCGAGGCCTTCCTCAAAACTCACGACGTTATTTTTGCCGACCGGCCCAAAATGGCGGCGGGAAAGTACACCACCTACAACTACTCCGACATCACTTGGTCCCCTTATGGCCCATATTGGCGCCAGGCCCGTAAAATGTGCATCATGGAACTTTTCAGCAACAAACGCCTACAGTCATATGAATACATAAGAAGAGAAGAAATGAGTGCTTTGCTTAGAGGCTTGTATGAGTCCTCCAACACTGAGCAAAATGTTAAAGACCACCTCTCCACTGTGAGTCTCAATGTCATAAGCCGAATGGTGCTCGGAAAGAAGTACACGGACGAGTCCAAGGATGCGATAGTGAGCCCCGATGAGTTCAAGAAATTGCTTGATGAGCTGTTCTTGCTGAGTGGGGTGTTGAATATTGGGGATTTCATTCCTTGGCTTGACATCTTGGACTTGCAGGGGTATATAAAGCGAATGAAGGCTTTAAGCAAGAAGTTGGACAGGTTCTTGGAGCATGTGTTGGATGAGCATATTGAAAATAAGAAGGCAAAAGGCTTTGTTGCAACAGATATGGTGGATGTGCTTTTGCAGCTTGCTGATGATCCCGACCTTGAAGTTAAGCTTGGGAGGCATGGGGTCAAGGCCTTTACGCAGGACCTAATTGCTGGTGGGACAGAGAGCTCAGCCGTGACAGTGGAGTGGGCAATCTCGGAGCTTCTAAGAAAGCCAGAGATTTTCAGGAAGGCAACCGAGGAGCTAGACAGGGTGATTGGAAGGGAAAGATGGGTGGAAGAGAGAGACATTGTTCACTTGCCATTCATTGATGCCATTGCCAAGGAAACCATGAGGCTTCACCCTGTGGCACCCATGTTGGTGCCAAGACTAGCACGTGAAGATTGCCAAGTTGCTGGGTATAACATTCCTAAGGGCACCAGAGTCCTAGTGAGTGTGTGGACAATAGGAAGAGACCCTAACTTGTGGGACAATCCCAATGAGTTCTGCCCGGAAAGGTTCATTGGCAAGGACATTGATGTCAAAGGCCAAGACTTTGAGCTGTTGCCATTTGGATCAGGAAGGAGGATGTGCCCTGGTTACAGTCTTGGCATTAAGGTTATTCAATCAAGTCTGGCTAATATCTTACATGGGTTCACATGGAGATTGCCTGGGAACATGAAGAAAGAGGATTTGAGTATGGAGGAAATTTTTGGGCTTTCGACACCCAGGAAGTTTCCCCTTGTTGCAGTCGCTGAGCCTCGTCTTCCACCTTATCTTTACTCCCAGTGA

Protein Analysis

508

Amino Acids

57.82

Weight (kDa)

8.42

Isoelectric Point (pI)

41.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
p450 PF00067 34 - 485 3.3e-98 Cytochrome P450
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1274
AccB1I GGYRCC 4 cut(s) 375, 1105, 1117, 1172
AccII CGCG 1 cut(s) 35
AciI CCGC 8 cut(s) 30, 33, 70, 79, 82, 101, 311, 314
AclWI GGATC 2 cut(s) 839, 1318
AcoI YGGCCR 1 cut(s) 255
AcsI RAATTY 2 cut(s) 212, 1443
AcvI CACGTG 1 cut(s) 1133
AcyI GRCGYC 1 cut(s) 376
AdeI CACNNNGTG 1 cut(s) 1102
AfaI GTAC 3 cut(s) 99, 323, 572
AfiI CCNNNNNNNGG 2 cut(s) 36, 128
AgsI TTSAA 5 cut(s) 616, 658, 779, 857, 1361
AjnI CCWGG 4 cut(s) 378, 1330, 1405, 1463
AjuI GAANNNNNNNTTGG 4 cut(s) 849, 881, 1242, 1274
AluBI AGCT 9 cut(s) 47, 208, 634, 835, 865, 924, 958, 1000, 1297
AluI AGCT 9 cut(s) 47, 208, 634, 835, 865, 924, 958, 1000, 1297
Alw21I GWGCWC 3 cut(s) 49, 561, 926
Alw26I GTCTC 3 cut(s) 539, 1035, 1203
AlwI GGATC 2 cut(s) 839, 1318
AlwNI CAGNNNCTG 1 cut(s) 1490
ApeKI GCWGC 2 cut(s) 205, 832
ApoI RAATTY 2 cut(s) 212, 1443
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
AspLEI GCGC 1 cut(s) 378
AspS9I GGNCC 9 cut(s) 107, 120, 194, 219, 300, 354, 365, 382, 898
AsuC2I CCSGG 2 cut(s) 105, 1247
AsuHPI GGTGA 3 cut(s) 152, 1021, 1088
AvaII GGWCC 4 cut(s) 194, 219, 354, 898
AxyI CCTNAGG 1 cut(s) 1167
BaeGI GKGCMC 2 cut(s) 1175, 1331
BanI GGYRCC 4 cut(s) 375, 1105, 1117, 1172
BanII GRGCYC 3 cut(s) 49, 605, 926
BbrPI CACGTG 1 cut(s) 1133
BbsI GAAGAC 1 cut(s) 1493
Bbv12I GWGCWC 3 cut(s) 49, 561, 926
BbvI GCAGC 2 cut(s) 217, 844
BccI CCATC 4 cut(s) 81, 131, 206, 1020
BceAI ACGGC 1 cut(s) 914
BciT130I CCWGG 4 cut(s) 380, 1332, 1407, 1465
BcnI CCSGG 2 cut(s) 105, 1247
BcoDI GTCTC 3 cut(s) 539, 1035, 1203
BfaI CTAG 4 cut(s) 50, 1001, 1127, 1184
BfmI CTRYAG 1 cut(s) 425
BfoI RGCGCY 1 cut(s) 379
BisI GCNGC 6 cut(s) 30, 33, 82, 206, 312, 833
BlpI GCTNAGC 2 cut(s) 925, 1491
BlsI GCNGC 6 cut(s) 31, 34, 83, 207, 313, 834
Bme1390I CCNGG 6 cut(s) 105, 380, 1247, 1332, 1407, 1465
Bme18I GGWCC 4 cut(s) 194, 219, 354, 898
BmgT120I GGNCC 9 cut(s) 107, 120, 194, 219, 300, 354, 365, 382, 898
BmiI GGNNCC 7 cut(s) 196, 220, 356, 377, 1107, 1119, 1174
BmrFI CCNGG 6 cut(s) 105, 380, 1247, 1332, 1407, 1465
BmrI ACTGGG 1 cut(s) 1516
BmsI GCATC 3 cut(s) 405, 580, 1057
BmuI ACTGGG 1 cut(s) 1516
BpiI GAAGAC 1 cut(s) 1493
Bpu1102I GCTNAGC 2 cut(s) 925, 1491
BpuMI CCSGG 2 cut(s) 105, 1247
BsaAI YACGTR 1 cut(s) 1133
BsaHI GRCGYC 1 cut(s) 376
BsaI GGTCTC 1 cut(s) 1203
BsaXI ACNNNNNCTCC 6 cut(s) 323, 353, 506, 536, 746, 776
Bsc4I CCNNNNNNNGG 2 cut(s) 36, 128
Bse118I RCCGGY 1 cut(s) 297
Bse1I ACTGG 1 cut(s) 1522
Bse21I CCTNAGG 1 cut(s) 1167
Bse3DI GCAATG 1 cut(s) 1071
BseBI CCWGG 4 cut(s) 380, 1332, 1407, 1465
BseGI GGATG 4 cut(s) 595, 772, 826, 1329
BseLI CCNNNNNNNGG 2 cut(s) 36, 128
BseMI GCAATG 1 cut(s) 1071
BseMII CTCAG 4 cut(s) 489, 635, 939, 1482
BseNI ACTGG 1 cut(s) 1522
BseRI GAGGAG 2 cut(s) 232, 1010
BseSI GKGCMC 2 cut(s) 1175, 1331
BseXI GCAGC 2 cut(s) 217, 844
BseYI CCCAGC 2 cut(s) 40, 1151
Bsh1236I CGCG 1 cut(s) 35
Bsh1285I CGRYCG 2 cut(s) 249, 298
BshNI GGYRCC 4 cut(s) 375, 1105, 1117, 1172
BsiEI CGRYCG 2 cut(s) 249, 298
BsiHKAI GWGCWC 3 cut(s) 49, 561, 926
BsiSI CCGG 3 cut(s) 105, 298, 1247
BslFI GGGAC 3 cut(s) 340, 928, 1238
BslI CCNNNNNNNGG 2 cut(s) 36, 128
BsmAI GTCTC 3 cut(s) 539, 1035, 1203
BsmFI GGGAC 3 cut(s) 340, 928, 1238
Bso31I GGTCTC 1 cut(s) 1203
Bsp1286I GDGCHC 6 cut(s) 49, 561, 605, 926, 1175, 1331
Bsp143I GATC 2 cut(s) 844, 1310
Bsp1720I GCTNAGC 2 cut(s) 925, 1491
BspACI CCGC 8 cut(s) 30, 33, 70, 79, 82, 101, 311, 314
BspCNI CTCAG 4 cut(s) 490, 636, 938, 1483
BspFNI CGCG 1 cut(s) 35
BspLI GGNNCC 7 cut(s) 196, 220, 356, 377, 1107, 1119, 1174
BspPI GGATC 2 cut(s) 839, 1318
BspT107I GGYRCC 4 cut(s) 375, 1105, 1117, 1172
BspTNI GGTCTC 1 cut(s) 1203
BsrDI GCAATG 1 cut(s) 1071
BsrFI RCCGGY 1 cut(s) 297
BsrI ACTGG 1 cut(s) 1522
BssAI RCCGGY 1 cut(s) 297
BssMI GATC 2 cut(s) 844, 1310
BssNI GRCGYC 1 cut(s) 376
BssT1I CCWWGG 4 cut(s) 116, 585, 677, 1077
Bst2UI CCWGG 4 cut(s) 380, 1332, 1407, 1465
Bst4CI ACNGT 5 cut(s) 164, 429, 529, 937, 1340
Bst6I CTCTTC 3 cut(s) 442, 1029, 1201
BstACI GRCGYC 1 cut(s) 376
BstBAI YACGTR 1 cut(s) 1133
BstC8I GCNNGC 1 cut(s) 837
BstDEI CTNAG 7 cut(s) 470, 498, 644, 925, 962, 1167, 1491
BstF5I GGATG 4 cut(s) 595, 772, 826, 1329
BstFNI CGCG 1 cut(s) 35
BstH2I RGCGCY 1 cut(s) 379
BstHHI GCGC 1 cut(s) 378
BstKTI GATC 2 cut(s) 847, 1313
BstMAI GTCTC 3 cut(s) 539, 1035, 1203
BstMBI GATC 2 cut(s) 844, 1310
BstMCI CGRYCG 2 cut(s) 249, 298
BstMWI GCNNNNNNNGC 6 cut(s) 78, 420, 631, 640, 832, 871
BstNI CCWGG 4 cut(s) 380, 1332, 1407, 1465
BstNSI RCATGY 1 cut(s) 761
BstSCI CCNGG 6 cut(s) 103, 378, 1245, 1330, 1405, 1463
BstSFI CTRYAG 1 cut(s) 425
BstSLI GKGCMC 2 cut(s) 1175, 1331
BstUI CGCG 1 cut(s) 35
BstV1I GCAGC 2 cut(s) 217, 844
BstV2I GAAGAC 1 cut(s) 1493
BstXI CCANNNNNNTGG 3 cut(s) 191, 235, 1151
Bsu36I CCTNAGG 1 cut(s) 1167
BtsCI GGATG 4 cut(s) 595, 772, 826, 1329
BtsI GCAGTG 1 cut(s) 9
BtsIMutI CAGTG 4 cut(s) 9, 495, 525, 942
Cac8I GCNNGC 1 cut(s) 837
CaiI CAGNNNCTG 1 cut(s) 1490
CfoI GCGC 1 cut(s) 378
Cfr10I RCCGGY 1 cut(s) 297
Cfr13I GGNCC 9 cut(s) 107, 120, 194, 219, 300, 354, 365, 382, 898
Csp6I GTAC 3 cut(s) 98, 322, 571
CviQI GTAC 3 cut(s) 98, 322, 571
DdeI CTNAG 7 cut(s) 470, 498, 644, 925, 962, 1167, 1491
DinI GGCGCC 1 cut(s) 377
DpnI GATC 2 cut(s) 846, 1312
DpnII GATC 2 cut(s) 844, 1310
DraIII CACNNNGTG 1 cut(s) 1102
DrdI GACNNNNNNGTC 1 cut(s) 1274
DseDI GACNNNNNNGTC 1 cut(s) 1274
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 3 cut(s) 442, 1029, 1201
EarI CTCTTC 3 cut(s) 442, 1029, 1201
EciI GGCGGA 1 cut(s) 68
Ecl136II GAGCTC 2 cut(s) 47, 924
Eco130I CCWWGG 4 cut(s) 116, 585, 677, 1077
Eco147I AGGCCT 2 cut(s) 263, 887
Eco24I GRGCYC 3 cut(s) 49, 605, 926
Eco31I GGTCTC 1 cut(s) 1203
Eco47I GGWCC 4 cut(s) 194, 219, 354, 898
Eco53kI GAGCTC 2 cut(s) 47, 924
Eco72I CACGTG 1 cut(s) 1133
Eco81I CCTNAGG 1 cut(s) 1167
EcoICRI GAGCTC 2 cut(s) 47, 924
EcoO109I RGGNCCY 2 cut(s) 219, 898
EcoRII CCWGG 4 cut(s) 378, 1330, 1405, 1463
EcoT14I CCWWGG 4 cut(s) 116, 585, 677, 1077
EcoT38I GRGCYC 3 cut(s) 49, 605, 926
EgeI GGCGCC 1 cut(s) 377
EheI GGCGCC 1 cut(s) 377
ErhI CCWWGG 4 cut(s) 116, 585, 677, 1077
FalI AAGNNNNNCTT 2 cut(s) 849, 881
FaqI GGGAC 3 cut(s) 340, 928, 1238
FauI CCCGC 2 cut(s) 77, 307
FauNDI CATATG 1 cut(s) 433
Fnu4HI GCNGC 6 cut(s) 30, 33, 82, 206, 312, 833
FokI GGATG 4 cut(s) 602, 779, 833, 1336
FriOI GRGCYC 3 cut(s) 49, 605, 926
Fsp4HI GCNGC 6 cut(s) 30, 33, 82, 206, 312, 833
FspBI CTAG 4 cut(s) 50, 1001, 1127, 1184
GlaI GCGC 1 cut(s) 377
GluI GCNGC 6 cut(s) 30, 33, 82, 206, 312, 833
GsaI CCCAGC 2 cut(s) 44, 1155
HaeII RGCGCY 1 cut(s) 379
HapII CCGG 3 cut(s) 105, 298, 1247
HhaI GCGC 1 cut(s) 378
Hin1I GRCGYC 1 cut(s) 376
Hin6I GCGC 1 cut(s) 376
HinP1I GCGC 1 cut(s) 376
HindIII AAGCTT 1 cut(s) 863
HinfI GANTC 4 cut(s) 485, 532, 581, 1179
HpaII CCGG 3 cut(s) 105, 298, 1247
HphI GGTGA 3 cut(s) 152, 1021, 1088
Hpy166II GTNNAC 5 cut(s) 324, 573, 1051, 1197, 1392
Hpy188I TCNGA 4 cut(s) 131, 343, 563, 955
Hpy188III TCNNGA 5 cut(s) 278, 616, 848, 982, 1314
Hpy8I GTNNAC 5 cut(s) 324, 573, 1051, 1197, 1392
Hpy99I CGWCG 1 cut(s) 284
HpyAV CCTTC 7 cut(s) 232, 274, 715, 781, 979, 1011, 1311
HpyCH4III ACNGT 5 cut(s) 164, 429, 529, 937, 1340
HpyCH4IV ACGT 2 cut(s) 282, 1132
HpyCH4V TGCA 7 cut(s) 176, 205, 396, 700, 806, 832, 1484
HpyF10VI GCNNNNNNNGC 6 cut(s) 78, 420, 631, 640, 832, 871
HpyF3I CTNAG 7 cut(s) 470, 498, 644, 925, 962, 1167, 1491
HpySE526I ACGT 2 cut(s) 282, 1132
Hsp92I GRCGYC 1 cut(s) 376
HspAI GCGC 1 cut(s) 376
KasI GGCGCC 1 cut(s) 375
Kzo9I GATC 2 cut(s) 844, 1310
LmnI GCTCC 4 cut(s) 44, 754, 955, 997
Lsp1109I GCAGC 2 cut(s) 217, 844
LweI GCATC 3 cut(s) 405, 580, 1057
MaeI CTAG 4 cut(s) 50, 1001, 1127, 1184
MaeII ACGT 2 cut(s) 282, 1132
MaeIII GTNAC 2 cut(s) 931, 1334
MalI GATC 2 cut(s) 846, 1312
MboI GATC 2 cut(s) 844, 1310
MboII GAAGA 7 cut(s) 459, 464, 1046, 1148, 1218, 1429, 1493
MhlI GDGCHC 6 cut(s) 49, 561, 605, 926, 1175, 1331
MluCI AATT 4 cut(s) 212, 620, 903, 1443
Mly113I GGCGCC 1 cut(s) 376
MlyI GAGTC 4 cut(s) 494, 541, 590, 1188
MmeI TCCRAC 4 cut(s) 366, 516, 720, 744
MseI TTAA 4 cut(s) 510, 728, 861, 1350
MslI CAYNNNNRTG 2 cut(s) 233, 1115
MspA1I CMGCKG 1 cut(s) 208
MspI CCGG 3 cut(s) 105, 298, 1247
MspR9I CCNGG 6 cut(s) 105, 380, 1247, 1332, 1407, 1465
MvaI CCWGG 4 cut(s) 380, 1332, 1407, 1465
MvnI CGCG 1 cut(s) 35
MwoI GCNNNNNNNGC 6 cut(s) 78, 420, 631, 640, 832, 871
NarI GGCGCC 1 cut(s) 376
NciI CCSGG 2 cut(s) 105, 1247
NdeI CATATG 1 cut(s) 433
NdeII GATC 2 cut(s) 844, 1310
NlaIV GGNNCC 7 cut(s) 196, 220, 356, 377, 1107, 1119, 1174
NmeAIII GCCGAG 1 cut(s) 283
NmuCI GTSAC 1 cut(s) 931
NspI RCATGY 1 cut(s) 761
PceI AGGCCT 2 cut(s) 263, 887
PcsI WCGNNNNNNNCGW 1 cut(s) 255
PkrI GCNGC 6 cut(s) 31, 34, 83, 207, 313, 834
PleI GAGTC 4 cut(s) 493, 540, 589, 1187
PluTI GGCGCC 1 cut(s) 379
PmaCI CACGTG 1 cut(s) 1133
PmlI CACGTG 1 cut(s) 1133
PpsI GAGTC 4 cut(s) 493, 540, 589, 1187
Ppu21I YACGTR 1 cut(s) 1133
PpuMI RGGWCCY 2 cut(s) 219, 898
Psp124BI GAGCTC 2 cut(s) 49, 926
Psp5II RGGWCCY 2 cut(s) 219, 898
Psp6I CCWGG 4 cut(s) 378, 1330, 1405, 1463
PspCI CACGTG 1 cut(s) 1133
PspFI CCCAGC 2 cut(s) 40, 1151
PspGI CCWGG 4 cut(s) 378, 1330, 1405, 1463
PspN4I GGNNCC 7 cut(s) 196, 220, 356, 377, 1107, 1119, 1174
PspPI GGNCC 9 cut(s) 107, 120, 194, 219, 300, 354, 365, 382, 898
PspPPI RGGWCCY 2 cut(s) 219, 898
PstNI CAGNNNCTG 1 cut(s) 1490
PvuII CAGCTG 1 cut(s) 208
RsaI GTAC 3 cut(s) 99, 323, 572
RsaNI GTAC 3 cut(s) 98, 322, 571
RseI CAYNNNNRTG 2 cut(s) 233, 1115
SacI GAGCTC 2 cut(s) 49, 926
SaqAI TTAA 4 cut(s) 510, 728, 861, 1350
SatI GCNGC 6 cut(s) 30, 33, 82, 206, 312, 833
Sau3AI GATC 2 cut(s) 844, 1310
Sau96I GGNCC 9 cut(s) 107, 120, 194, 219, 300, 354, 365, 382, 898
SchI GAGTC 4 cut(s) 494, 541, 590, 1188
ScrFI CCNGG 6 cut(s) 105, 380, 1247, 1332, 1407, 1465
SduI GDGCHC 6 cut(s) 49, 561, 605, 926, 1175, 1331
SfaNI GCATC 3 cut(s) 405, 580, 1057
SfcI CTRYAG 1 cut(s) 425
SfoI GGCGCC 1 cut(s) 377
SinI GGWCC 4 cut(s) 194, 219, 354, 898
SmiMI CAYNNNNRTG 2 cut(s) 233, 1115
Sse9I AATT 4 cut(s) 212, 620, 903, 1443
SseBI AGGCCT 2 cut(s) 263, 887
SsiI CCGC 8 cut(s) 30, 33, 70, 79, 82, 101, 311, 314
SspDI GGCGCC 1 cut(s) 375
SspI AATATT 1 cut(s) 661
SspMI CTAG 4 cut(s) 50, 1001, 1127, 1184
SstI GAGCTC 2 cut(s) 49, 926
StuI AGGCCT 2 cut(s) 263, 887
StyD4I CCNGG 6 cut(s) 103, 378, 1245, 1330, 1405, 1463
StyI CCWWGG 4 cut(s) 116, 585, 677, 1077
TaaI ACNGT 5 cut(s) 164, 429, 529, 937, 1340
TaiI ACGT 2 cut(s) 285, 1135
TaqI TCGA 2 cut(s) 249, 1457
TaqII GACCGA 1 cut(s) 235
TasI AATT 4 cut(s) 212, 620, 903, 1443
TatI WGTACW 2 cut(s) 321, 570
TauI GCSGC 4 cut(s) 32, 35, 84, 314
Tru1I TTAA 4 cut(s) 510, 728, 861, 1350
Tru9I TTAA 4 cut(s) 510, 728, 861, 1350
TscAI CASTG 4 cut(s) 16, 502, 532, 942
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 2 cut(s) 205, 832
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 6 cut(s) 450, 661, 734, 1051, 1246, 1430
TspGWI ACGGA 1 cut(s) 590
TspRI CASTG 4 cut(s) 16, 502, 532, 942
VpaK11BI GGWCC 4 cut(s) 194, 219, 354, 898
XapI RAATTY 2 cut(s) 212, 1443
XceI RCATGY 1 cut(s) 761
XspI CTAG 4 cut(s) 50, 1001, 1127, 1184
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.