Rmu_sc0001802.1_g000001

THO complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001802.1
OG0006099
Physical Location & Seq
Reverse (-)
2493 .. 7967
5475 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1110 bp
atggttgtcctaaatacctttgaagctcaaccattaagtgatgaagagggagatgccaataatttggaggagtcagcaaatttcagcataaagtatcttacaagtagtaagctgatgggtttagagttaaaggatccgagtttccgacgacacattcttgtgcagtgcctcatattgttcgattatctgaaggccccaggaaaaagtgaaaaagatttgccttctgaaagcatgaaagaagaaataaattcttatgagcagcacgtgaagaaactgcttaaaatgactccaccaaagggggaaagttttcttaataaaattgagcatatattagaacgtgaaaagaattgggtatggtggaaacgtgatggttgcccgccttttgagaagcagccaatagagaagaaaacggttcaagatggagccaaaaagcgaaagccgaggtggagattgggaaataaagaactctcccagttgtggaagtgggcagatcagaatccgaatgctttaactgatcctcaacgtcttcagacccctgccatcactgagtattggaaacccttggctgaggacatggatccagcagctggaatagaagctgaatatcaccacaaaaataaccgagtttactgctggaaaggtcttcgattttcagctagacaagacttggaaggattttctaaatttactgaatatggtattgaaggagtggtacctctagaacttctgcctcctgaagaacgtgctaaatacactcctaaaccaaatgacaggtccaaacgtgctaaaaaggaagatacaaagggtgctgcgcatcaagtagaagaaaatcagatggcaacggctgcaactgaggtagatggtgaaggactcagaactgatgttggggcctttgtggctccattggacactgataacaccgccacggttggcaatacgtcccaaggtagcagtccaatggcagatgaacatcagaagcaaagttctgatactgatggtggtcaagaggcagggcagttggaagacgatgctgaggtggatgccgagggggatgccgggatgattgatggagagatggaaccggaggttgatttagatgcagttagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000160 GO:0000347 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006139 GO:0006396 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006950 GO:0006952 GO:0007154 GO:0007165 GO:0008104 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009620 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009873 GO:0009892 GO:0009987 GO:0010033 GO:0010267 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016246 GO:0016441 GO:0016458 GO:0019222 GO:0023052 GO:0030422 GO:0031047 GO:0031050 GO:0031503 GO:0032870 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0035194 GO:0035556 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046483 GO:0046907 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0050832 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051704 GO:0051707 GO:0051716 GO:0060255 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071369 GO:0071407 GO:0071426 GO:0071427 GO:0071495 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0098542 GO:1901360 GO:1901698 GO:1901699
KEGG Pathways
Metabolic & Signaling
ko03013 ko03040 map03013 map03040
Pfam Domains
Protein Families
efThoc1

Protein Analysis

369

Amino Acids

41.58

Weight (kDa)

4.89

Isoelectric Point (pI)

50.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
efThoc1 PF11957 22 - 259 8.4e-50 THO complex subunit 1 transcription elongation factor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 813
Acc65I GGTACC 1 cut(s) 712
AccB1I GGYRCC 1 cut(s) 712
AccB7I CCANNNNNTGG 1 cut(s) 587
AciI CCGC 2 cut(s) 377, 921
AclWI GGATC 5 cut(s) 128, 141, 509, 572, 585
AcsI RAATTY 3 cut(s) 79, 247, 683
AcuI CTGAAG 3 cut(s) 209, 512, 756
AcvI CACGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 714
AfiI CCNNNNNNNGG 3 cut(s) 296, 477, 587
AgsI TTSAA 3 cut(s) 23, 416, 704
AjnI CCWGG 1 cut(s) 196
AluBI AGCT 6 cut(s) 26, 112, 587, 599, 656, 1107
AluI AGCT 6 cut(s) 26, 112, 587, 599, 656, 1107
AlwI GGATC 5 cut(s) 128, 141, 509, 572, 585
AlwNI CAGNNNCTG 1 cut(s) 587
AoxI GGCC 2 cut(s) 192, 888
ApeKI GCWGC 5 cut(s) 259, 391, 584, 809, 845
ApoI RAATTY 3 cut(s) 79, 247, 683
ArsI GACNNNNNNTTYG 2 cut(s) 758, 790
Asp700I GAANNNNTTC 1 cut(s) 306
Asp718I GGTACC 1 cut(s) 712
AspLEI GCGC 1 cut(s) 814
AspS9I GGNCC 3 cut(s) 193, 774, 888
AsuC2I CCSGG 1 cut(s) 1057
AsuHPI GGTGA 2 cut(s) 599, 875
AvaII GGWCC 1 cut(s) 774
BamHI GGATCC 2 cut(s) 133, 577
BanI GGYRCC 1 cut(s) 712
BarI GAAGNNNNNNTAC 2 cut(s) 696, 728
BbrPI CACGTG 1 cut(s) 265
BbsI GAAGAC 3 cut(s) 518, 635, 1029
BbvCI CCTCAGC 2 cut(s) 567, 1032
BbvI GCAGC 5 cut(s) 271, 403, 596, 796, 832
BccI CCATC 9 cut(s) 109, 362, 413, 548, 829, 854, 989, 1061, 1069
BceAI ACGGC 1 cut(s) 858
BcgI CGANNNNNNTGC 4 cut(s) 612, 646, 1034, 1068
BciT130I CCWGG 1 cut(s) 198
BcnI CCSGG 1 cut(s) 1057
BfaI CTAG 2 cut(s) 657, 719
BglI GCCNNNNNGGC 1 cut(s) 896
BisI GCNGC 5 cut(s) 260, 392, 585, 810, 846
BlsI GCNGC 5 cut(s) 261, 393, 586, 811, 847
Bme1390I CCNGG 2 cut(s) 198, 1057
Bme18I GGWCC 1 cut(s) 774
BmgT120I GGNCC 3 cut(s) 193, 774, 888
BmiI GGNNCC 8 cut(s) 135, 195, 424, 579, 714, 889, 900, 1080
BmrFI CCNGG 2 cut(s) 198, 1057
BmrI ACTGGG 1 cut(s) 466
BmsI GCATC 6 cut(s) 43, 823, 1018, 1030, 1042, 1087
BmuI ACTGGG 1 cut(s) 466
BpiI GAAGAC 3 cut(s) 518, 635, 1029
Bpu10I CCTNAGC 2 cut(s) 567, 1032
BpuMI CCSGG 1 cut(s) 1057
BsaAI YACGTR 1 cut(s) 265
BsaBI GATNNNNATC 2 cut(s) 495, 969
BsaJI CCNNGG 6 cut(s) 196, 440, 561, 924, 943, 1044
BsaWI WCCGGW 1 cut(s) 1081
Bsc4I CCNNNNNNNGG 3 cut(s) 296, 477, 587
Bse1I ACTGG 1 cut(s) 472
Bse8I GATNNNNATC 2 cut(s) 495, 969
BseBI CCWGG 1 cut(s) 198
BseDI CCNNGG 6 cut(s) 196, 440, 561, 924, 943, 1044
BseGI GGATG 3 cut(s) 1045, 1057, 1065
BseJI GATNNNNATC 2 cut(s) 495, 969
BseLI CCNNNNNNNGG 3 cut(s) 296, 477, 587
BseMII CTCAG 5 cut(s) 537, 558, 843, 886, 1023
BseNI ACTGG 1 cut(s) 472
BseRI GAGGAG 1 cut(s) 83
BseXI GCAGC 5 cut(s) 271, 403, 596, 796, 832
BsgI GTGCAG 1 cut(s) 182
BshFI GGCC 2 cut(s) 194, 890
BshNI GGYRCC 1 cut(s) 712
BsiSI CCGG 2 cut(s) 1056, 1082
BslFI GGGAC 1 cut(s) 925
BslI CCNNNNNNNGG 3 cut(s) 296, 477, 587
BsmFI GGGAC 1 cut(s) 925
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 2 cut(s) 194, 890
Bsp143I GATC 4 cut(s) 133, 490, 514, 577
BspACI CCGC 2 cut(s) 377, 921
BspANI GGCC 2 cut(s) 194, 890
BspCNI CTCAG 5 cut(s) 538, 559, 844, 885, 1024
BspLI GGNNCC 8 cut(s) 135, 195, 424, 579, 714, 889, 900, 1080
BspPI GGATC 5 cut(s) 128, 141, 509, 572, 585
BspT107I GGYRCC 1 cut(s) 712
BsrI ACTGG 1 cut(s) 472
BssECI CCNNGG 6 cut(s) 196, 440, 561, 924, 943, 1044
BssMI GATC 4 cut(s) 133, 490, 514, 577
BssT1I CCWWGG 2 cut(s) 561, 943
Bst2UI CCWGG 1 cut(s) 198
Bst4CI ACNGT 2 cut(s) 412, 928
Bst6I CTCTTC 1 cut(s) 39
BstAPI GCANNNNNTGC 1 cut(s) 845
BstBAI YACGTR 1 cut(s) 265
BstC8I GCNNGC 1 cut(s) 377
BstDEI CTNAG 5 cut(s) 546, 567, 852, 872, 1032
BstDSI CCRYGG 1 cut(s) 924
BstF5I GGATG 3 cut(s) 1045, 1057, 1065
BstHHI GCGC 1 cut(s) 814
BstKTI GATC 4 cut(s) 136, 493, 517, 580
BstMBI GATC 4 cut(s) 133, 490, 514, 577
BstMWI GCNNNNNNNGC 2 cut(s) 845, 896
BstNI CCWGG 1 cut(s) 198
BstSCI CCNGG 2 cut(s) 196, 1055
BstV1I GCAGC 5 cut(s) 271, 403, 596, 796, 832
BstV2I GAAGAC 3 cut(s) 518, 635, 1029
BstX2I RGATCY 2 cut(s) 133, 577
BstXI CCANNNNNNTGG 1 cut(s) 64
BstYI RGATCY 2 cut(s) 133, 577
BsuRI GGCC 2 cut(s) 194, 890
BtgI CCRYGG 1 cut(s) 924
BtsCI GGATG 3 cut(s) 1045, 1057, 1065
BtsI GCAGTG 1 cut(s) 170
BtsIMutI CAGTG 3 cut(s) 170, 543, 909
Cac8I GCNNGC 1 cut(s) 377
CaiI CAGNNNCTG 1 cut(s) 587
CfoI GCGC 1 cut(s) 814
Cfr13I GGNCC 3 cut(s) 193, 774, 888
Csp6I GTAC 1 cut(s) 713
CviAII CATG 2 cut(s) 232, 574
CviQI GTAC 1 cut(s) 713
DdeI CTNAG 5 cut(s) 546, 567, 852, 872, 1032
DpnI GATC 4 cut(s) 135, 492, 516, 579
DpnII GATC 4 cut(s) 133, 490, 514, 577
Eam1104I CTCTTC 1 cut(s) 39
EarI CTCTTC 1 cut(s) 39
Eco130I CCWWGG 2 cut(s) 561, 943
Eco47I GGWCC 1 cut(s) 774
Eco57I CTGAAG 3 cut(s) 209, 512, 756
Eco72I CACGTG 1 cut(s) 265
EcoO109I RGGNCCY 2 cut(s) 193, 888
EcoRII CCWGG 1 cut(s) 196
EcoT14I CCWWGG 2 cut(s) 561, 943
ErhI CCWWGG 2 cut(s) 561, 943
FaeI CATG 2 cut(s) 235, 577
FaiI YATR 9 cut(s) 89, 173, 233, 255, 327, 329, 355, 575, 696
FaqI GGGAC 1 cut(s) 925
FatI CATG 2 cut(s) 231, 573
FauI CCCGC 1 cut(s) 384
Fnu4HI GCNGC 5 cut(s) 260, 392, 585, 810, 846
FokI GGATG 3 cut(s) 1052, 1064, 1072
Fsp4HI GCNGC 5 cut(s) 260, 392, 585, 810, 846
FspBI CTAG 2 cut(s) 657, 719
FspI TGCGCA 1 cut(s) 813
GlaI GCGC 1 cut(s) 813
GluI GCNGC 5 cut(s) 260, 392, 585, 810, 846
HaeIII GGCC 2 cut(s) 194, 890
HapII CCGG 2 cut(s) 1056, 1082
HhaI GCGC 1 cut(s) 814
Hin1II CATG 2 cut(s) 235, 577
Hin6I GCGC 1 cut(s) 812
HinP1I GCGC 1 cut(s) 812
HinfI GANTC 4 cut(s) 71, 286, 496, 870
HpaII CCGG 2 cut(s) 1056, 1082
HphI GGTGA 2 cut(s) 599, 875
Hpy166II GTNNAC 1 cut(s) 628
Hpy188III TCNNGA 4 cut(s) 416, 719, 734, 1004
Hpy8I GTNNAC 1 cut(s) 628
Hpy99I CGWCG 1 cut(s) 150
HpyAV CCTTC 5 cut(s) 184, 231, 665, 698, 860
HpyCH4III ACNGT 2 cut(s) 412, 928
HpyCH4IV ACGT 7 cut(s) 264, 337, 364, 523, 742, 781, 938
HpyCH4V TGCA 3 cut(s) 163, 848, 1100
HpyF10VI GCNNNNNNNGC 2 cut(s) 845, 896
HpyF3I CTNAG 5 cut(s) 546, 567, 852, 872, 1032
HpySE526I ACGT 7 cut(s) 264, 337, 364, 523, 742, 781, 938
Hsp92II CATG 2 cut(s) 235, 577
HspAI GCGC 1 cut(s) 812
KpnI GGTACC 1 cut(s) 716
Kzo9I GATC 4 cut(s) 133, 490, 514, 577
LmnI GCTCC 2 cut(s) 422, 904
Lsp1109I GCAGC 5 cut(s) 271, 403, 596, 796, 832
LweI GCATC 6 cut(s) 43, 823, 1018, 1030, 1042, 1087
MaeI CTAG 2 cut(s) 657, 719
MaeII ACGT 7 cut(s) 264, 337, 364, 523, 742, 781, 938
MalI GATC 4 cut(s) 135, 492, 516, 579
MboI GATC 4 cut(s) 133, 490, 514, 577
MflI RGATCY 2 cut(s) 133, 577
MluCI AATT 6 cut(s) 61, 79, 247, 318, 346, 683
MlyI GAGTC 3 cut(s) 80, 280, 864
MmeI TCCRAC 2 cut(s) 169, 999
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 5 cut(s) 35, 128, 279, 312, 509
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 587
MspI CCGG 2 cut(s) 1056, 1082
MspR9I CCNGG 2 cut(s) 198, 1057
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 1 cut(s) 198
MwoI GCNNNNNNNGC 2 cut(s) 845, 896
NciI CCSGG 1 cut(s) 1057
NdeII GATC 4 cut(s) 133, 490, 514, 577
NlaIII CATG 2 cut(s) 235, 577
NlaIV GGNNCC 8 cut(s) 135, 195, 424, 579, 714, 889, 900, 1080
NmeAIII GCCGAG 2 cut(s) 465, 1069
NsbI TGCGCA 1 cut(s) 813
PctI GAATGC 1 cut(s) 508
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 1 cut(s) 496
PflMI CCANNNNNTGG 1 cut(s) 587
PkrI GCNGC 5 cut(s) 261, 393, 586, 811, 847
PleI GAGTC 3 cut(s) 79, 280, 864
PmaCI CACGTG 1 cut(s) 265
PmlI CACGTG 1 cut(s) 265
PpsI GAGTC 3 cut(s) 79, 280, 864
Ppu21I YACGTR 1 cut(s) 265
Psp6I CCWGG 1 cut(s) 196
PspCI CACGTG 1 cut(s) 265
PspGI CCWGG 1 cut(s) 196
PspN4I GGNNCC 8 cut(s) 135, 195, 424, 579, 714, 889, 900, 1080
PspPI GGNCC 3 cut(s) 193, 774, 888
PstNI CAGNNNCTG 1 cut(s) 587
PsuI RGATCY 2 cut(s) 133, 577
PvuII CAGCTG 1 cut(s) 587
RsaI GTAC 1 cut(s) 714
RsaNI GTAC 1 cut(s) 713
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 5 cut(s) 35, 128, 279, 312, 509
SatI GCNGC 5 cut(s) 260, 392, 585, 810, 846
Sau3AI GATC 4 cut(s) 133, 490, 514, 577
Sau96I GGNCC 3 cut(s) 193, 774, 888
SchI GAGTC 3 cut(s) 80, 280, 864
ScrFI CCNGG 2 cut(s) 198, 1057
SfaNI GCATC 6 cut(s) 43, 823, 1018, 1030, 1042, 1087
SinI GGWCC 1 cut(s) 774
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 6 cut(s) 61, 79, 247, 318, 346, 683
SsiI CCGC 2 cut(s) 377, 921
SspMI CTAG 2 cut(s) 657, 719
StyD4I CCNGG 2 cut(s) 196, 1055
StyI CCWWGG 2 cut(s) 561, 943
TaaI ACNGT 2 cut(s) 412, 928
TaiI ACGT 7 cut(s) 267, 340, 367, 526, 745, 784, 941
TaqI TCGA 2 cut(s) 180, 646
TasI AATT 6 cut(s) 61, 79, 247, 318, 346, 683
TfiI GAWTC 1 cut(s) 496
Tru1I TTAA 5 cut(s) 35, 128, 279, 312, 509
Tru9I TTAA 5 cut(s) 35, 128, 279, 312, 509
TscAI CASTG 3 cut(s) 170, 550, 916
TseI GCWGC 5 cut(s) 259, 391, 584, 809, 845
TspDTI ATGAA 3 cut(s) 57, 248, 981
TspRI CASTG 3 cut(s) 170, 550, 916
Van91I CCANNNNNTGG 1 cut(s) 587
VpaK11BI GGWCC 1 cut(s) 774
XapI RAATTY 3 cut(s) 79, 247, 683
XbaI TCTAGA 1 cut(s) 718
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 2 cut(s) 657, 719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.