Rmu_sc0001922.1_g000010

Aminomethyltransferase folate-binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001922.1
OG0005067
Physical Location & Seq
Reverse (-)
63025 .. 68037
5013 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1341 bp
atggtccccggatttcatacaaccttggatcaatgtgttccaagagaagccatttctatggggcatttcatcaaacaagttgagtgcatcattcatgctgccacatttaacatacatctgcagaagccggtttccgaatgtaacagtggagttgatgagacccttcttgagaaaaacaagacgaagaagcagcagaagctcacactcttctcatcttccaaaacggggaccagggctttgccattcgacctctcccctcctccgattgaccacgacctcttggacactgtggcagcaactgcaggagcaaaagtctcagatgatgggattatcgaaacgtttgataacgacgaccaagctttagatgctgtcgataatggtgttgcggttgtggacctttcacatttttgccggattagagttagcggagaagaccgcatccagtttcttcacaaccaaagcacggccaatttcgaatgccttgatgaaggacagggatgtgacaccgtttttgttacaccaacagctcggacaatagatatcgctcatgcatgggtcatgaaaaatgcagtaatgttaatggtttcacctgtttccagaagtatctctgaaatgctgaaaaagtacatattttttaatgacaaggtagagattcaagacatcactaagcaaacttgtttcttcgttttagtgggacctaaaagcaatcaacttatggaggagttaaacctaggtgatctcgttggacaaccatatggcacccatcagcatttcagtgtcaatgggatgcctgtaactgtgggagtgggcaatgtgatatctgaaaaaggcttttcacttttgatgtcaccagctgccgctggatcagtctggaaaactattctatcacaaggtgcaactccaatgggctctaatgcatgggaaaaactaagaattctccaaggaaagccagctcctcagaaggagcttactaatgaatataatgtcctggaggctggtctctggaattcgatctccttaagcaaagggtgctataagggacaagagactatatcgagactgataacgtatgatggagtcaagcaaagactttggggaatctctctatcagcgccagcagaacctggcagcatcattacagttggtgggaaaaaggttggaaagctaacaagttgcacatctggaagaaaggaaagcgagtattttggtttaggctatatcaagaggcaaaatgtttcagaaggagacacgctaatggttggagatgctattactggtacagtggtagaagtaccttttcttgctgggcaacggccaccatcgagaagctcaagctcctga

Protein Analysis

446

Amino Acids

48.43

Weight (kDa)

5.96

Isoelectric Point (pI)

40.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCV_T PF01571 118 - 342 3e-27 GCVT N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 758
AciI CCGC 4 cut(s) 386, 426, 436, 858
AclI AACGTT 1 cut(s) 338
AclWI GGATC 2 cut(s) 36, 871
AcoI YGGCCR 2 cut(s) 465, 1313
AcsI RAATTY 2 cut(s) 933, 1006
AdeI CACNNNGTG 1 cut(s) 893
AfaI GTAC 3 cut(s) 626, 1279, 1293
AfiI CCNNNNNNNGG 2 cut(s) 225, 463
AflII CTTAAG 1 cut(s) 1018
AgsI TTSAA 1 cut(s) 656
AjnI CCWGG 3 cut(s) 230, 987, 1123
AluBI AGCT 9 cut(s) 199, 359, 527, 854, 953, 967, 1165, 1329, 1335
AluI AGCT 9 cut(s) 199, 359, 527, 854, 953, 967, 1165, 1329, 1335
Alw26I GTCTC 6 cut(s) 152, 319, 1004, 1040, 1051, 1239
AlwI GGATC 2 cut(s) 36, 871
AlwNI CAGNNNCTG 2 cut(s) 299, 1124
AoxI GGCC 2 cut(s) 465, 1313
ApeKI GCWGC 5 cut(s) 98, 190, 293, 854, 1128
ApoI RAATTY 2 cut(s) 933, 1006
ArsI GACNNNNNNTTYG 4 cut(s) 220, 252, 494, 526
AspA2I CCTAGG 1 cut(s) 730
AspLEI GCGC 1 cut(s) 1114
AspS9I GGNCC 4 cut(s) 4, 228, 394, 695
AsuC2I CCSGG 1 cut(s) 9
AsuHPI GGTGA 3 cut(s) 579, 746, 840
AsuII TTCGAA 1 cut(s) 474
AvaII GGWCC 4 cut(s) 4, 228, 394, 695
AvrII CCTAGG 1 cut(s) 730
BaeI ACNNNNGTAYC 2 cut(s) 1269, 1302
BanI GGYRCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 911
BbsI GAAGAC 1 cut(s) 438
BbvI GCAGC 5 cut(s) 85, 202, 305, 841, 1140
BccI CCATC 4 cut(s) 317, 771, 1067, 1327
BceAI ACGGC 2 cut(s) 480, 1328
BciT130I CCWGG 3 cut(s) 232, 989, 1125
BcnI CCSGG 1 cut(s) 9
BcoDI GTCTC 6 cut(s) 152, 319, 1004, 1040, 1051, 1239
BfaI CTAG 1 cut(s) 731
BfmI CTRYAG 2 cut(s) 119, 300
BfoI RGCGCY 1 cut(s) 1115
BfrI CTTAAG 1 cut(s) 1018
BisI GCNGC 6 cut(s) 99, 191, 294, 855, 858, 1129
BlnI CCTAGG 1 cut(s) 730
BlsI GCNGC 6 cut(s) 100, 192, 295, 856, 859, 1130
Bme1390I CCNGG 4 cut(s) 9, 232, 989, 1125
Bme18I GGWCC 4 cut(s) 4, 228, 394, 695
BmgT120I GGNCC 4 cut(s) 4, 228, 394, 695
BmiI GGNNCC 4 cut(s) 6, 229, 696, 760
BmrFI CCNGG 4 cut(s) 9, 232, 989, 1125
BmsI GCATC 6 cut(s) 96, 355, 447, 777, 1140, 1255
BpiI GAAGAC 1 cut(s) 438
BpmI CTGGAG 1 cut(s) 1010
Bpu14I TTCGAA 1 cut(s) 474
BpuEI CTTGAG 2 cut(s) 188, 1315
BpuMI CCSGG 1 cut(s) 9
BsaI GGTCTC 2 cut(s) 152, 1004
BsaJI CCNNGG 5 cut(s) 7, 24, 231, 730, 940
BsaXI ACNNNNNCTCC 2 cut(s) 297, 327
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 463
Bse118I RCCGGY 1 cut(s) 127
Bse1I ACTGG 2 cut(s) 442, 1279
Bse3DI GCAATG 1 cut(s) 817
BseBI CCWGG 3 cut(s) 232, 989, 1125
BseDI CCNNGG 5 cut(s) 7, 24, 231, 730, 940
BseGI GGATG 3 cut(s) 438, 503, 792
BseLI CCNNNNNNNGG 2 cut(s) 225, 463
BseMI GCAATG 1 cut(s) 817
BseMII CTCAG 2 cut(s) 330, 971
BseNI ACTGG 2 cut(s) 442, 1279
BseRI GAGGAG 3 cut(s) 249, 734, 945
BseXI GCAGC 5 cut(s) 85, 202, 305, 841, 1140
BseYI CCCAGC 1 cut(s) 1304
BshFI GGCC 2 cut(s) 467, 1315
BshNI GGYRCC 1 cut(s) 758
BsiSI CCGG 3 cut(s) 9, 128, 412
BslFI GGGAC 3 cut(s) 241, 708, 1053
BslI CCNNNNNNNGG 2 cut(s) 225, 463
BsmAI GTCTC 6 cut(s) 152, 319, 1004, 1040, 1051, 1239
BsmFI GGGAC 3 cut(s) 241, 708, 1053
BsmI GAATGC 1 cut(s) 482
BsnI GGCC 2 cut(s) 467, 1315
Bso31I GGTCTC 2 cut(s) 152, 1004
Bsp119I TTCGAA 1 cut(s) 474
Bsp1286I GDGCHC 1 cut(s) 911
Bsp143I GATC 4 cut(s) 28, 736, 863, 1011
BspACI CCGC 4 cut(s) 386, 426, 436, 858
BspANI GGCC 2 cut(s) 467, 1315
BspCNI CTCAG 2 cut(s) 329, 970
BspHI TCATGA 1 cut(s) 558
BspLI GGNNCC 4 cut(s) 6, 229, 696, 760
BspMAI CTGCAG 2 cut(s) 123, 304
BspPI GGATC 2 cut(s) 36, 871
BspT104I TTCGAA 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 758
BspTI CTTAAG 1 cut(s) 1018
BspTNI GGTCTC 2 cut(s) 152, 1004
BsrDI GCAATG 1 cut(s) 817
BsrFI RCCGGY 1 cut(s) 127
BsrI ACTGG 2 cut(s) 442, 1279
BssAI RCCGGY 1 cut(s) 127
BssECI CCNNGG 5 cut(s) 7, 24, 231, 730, 940
BssMI GATC 4 cut(s) 28, 736, 863, 1011
BssT1I CCWWGG 3 cut(s) 24, 730, 940
Bst2UI CCWGG 3 cut(s) 232, 989, 1125
Bst4CI ACNGT 6 cut(s) 146, 289, 508, 799, 1141, 1282
Bst6I CTCTTC 1 cut(s) 212
BstAFI CTTAAG 1 cut(s) 1018
BstAPI GCANNNNNTGC 2 cut(s) 299, 1029
BstBI TTCGAA 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 951, 1116
BstDEI CTNAG 4 cut(s) 316, 666, 929, 957
BstF5I GGATG 3 cut(s) 438, 503, 792
BstH2I RGCGCY 1 cut(s) 1115
BstHHI GCGC 1 cut(s) 1114
BstKTI GATC 4 cut(s) 31, 739, 866, 1014
BstMAI GTCTC 6 cut(s) 152, 319, 1004, 1040, 1051, 1239
BstMBI GATC 4 cut(s) 28, 736, 863, 1011
BstMWI GCNNNNNNNGC 4 cut(s) 196, 299, 365, 1029
BstNI CCWGG 3 cut(s) 232, 989, 1125
BstSCI CCNGG 4 cut(s) 7, 230, 987, 1123
BstSFI CTRYAG 2 cut(s) 119, 300
BstV1I GCAGC 5 cut(s) 85, 202, 305, 841, 1140
BstV2I GAAGAC 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 58
BsuRI GGCC 2 cut(s) 467, 1315
BtsCI GGATG 3 cut(s) 438, 503, 792
BtsIMutI CAGTG 4 cut(s) 151, 285, 781, 1287
Cac8I GCNNGC 2 cut(s) 951, 1116
CaiI CAGNNNCTG 2 cut(s) 299, 1124
CciI TCATGA 1 cut(s) 558
CfoI GCGC 1 cut(s) 1114
Cfr10I RCCGGY 1 cut(s) 127
Cfr13I GGNCC 4 cut(s) 4, 228, 394, 695
Csp6I GTAC 3 cut(s) 625, 1278, 1292
CviAII CATG 5 cut(s) 95, 548, 552, 559, 918
CviQI GTAC 3 cut(s) 625, 1278, 1292
DdeI CTNAG 4 cut(s) 316, 666, 929, 957
DpnI GATC 4 cut(s) 30, 738, 865, 1013
DpnII GATC 4 cut(s) 28, 736, 863, 1011
DraIII CACNNNGTG 1 cut(s) 893
EaeI YGGCCR 2 cut(s) 465, 1313
Eam1104I CTCTTC 1 cut(s) 212
EarI CTCTTC 1 cut(s) 212
Eco130I CCWWGG 3 cut(s) 24, 730, 940
Eco24I GRGCYC 1 cut(s) 911
Eco31I GGTCTC 2 cut(s) 152, 1004
Eco32I GATATC 2 cut(s) 541, 819
Eco47I GGWCC 4 cut(s) 4, 228, 394, 695
EcoO109I RGGNCCY 1 cut(s) 695
EcoRI GAATTC 2 cut(s) 933, 1006
EcoRII CCWGG 3 cut(s) 230, 987, 1123
EcoRV GATATC 2 cut(s) 541, 819
EcoT14I CCWWGG 3 cut(s) 24, 730, 940
EcoT22I ATGCAT 2 cut(s) 553, 919
EcoT38I GRGCYC 1 cut(s) 911
ErhI CCWWGG 3 cut(s) 24, 730, 940
FaeI CATG 5 cut(s) 98, 551, 555, 562, 921
FaqI GGGAC 3 cut(s) 241, 708, 1053
FatI CATG 5 cut(s) 94, 547, 551, 558, 917
FauNDI CATATG 1 cut(s) 754
Fnu4HI GCNGC 6 cut(s) 99, 191, 294, 855, 858, 1129
FokI GGATG 3 cut(s) 425, 510, 799
FriOI GRGCYC 1 cut(s) 911
Fsp4HI GCNGC 6 cut(s) 99, 191, 294, 855, 858, 1129
FspBI CTAG 1 cut(s) 731
GlaI GCGC 1 cut(s) 1113
GluI GCNGC 6 cut(s) 99, 191, 294, 855, 858, 1129
GsaI CCCAGC 1 cut(s) 1308
GsuI CTGGAG 1 cut(s) 1010
HaeII RGCGCY 1 cut(s) 1115
HaeIII GGCC 2 cut(s) 467, 1315
HapII CCGG 3 cut(s) 9, 128, 412
HhaI GCGC 1 cut(s) 1114
Hin1II CATG 5 cut(s) 98, 551, 555, 562, 921
Hin6I GCGC 1 cut(s) 1112
HinP1I GCGC 1 cut(s) 1112
HindIII AAGCTT 1 cut(s) 357
HinfI GANTC 3 cut(s) 652, 1077, 1098
HpaII CCGG 3 cut(s) 9, 128, 412
HphI GGTGA 3 cut(s) 579, 746, 840
Hpy166II GTNNAC 1 cut(s) 394
Hpy188I TCNGA 8 cut(s) 136, 264, 319, 531, 610, 823, 960, 1240
Hpy8I GTNNAC 1 cut(s) 394
Hpy99I CGWCG 1 cut(s) 353
HpyAV CCTTC 4 cut(s) 173, 482, 955, 1235
HpyCH4III ACNGT 6 cut(s) 146, 289, 508, 799, 1141, 1282
HpyCH4IV ACGT 2 cut(s) 338, 1067
HpyCH4V TGCA 8 cut(s) 87, 121, 302, 551, 569, 896, 917, 1176
HpyF10VI GCNNNNNNNGC 4 cut(s) 196, 299, 365, 1029
HpyF3I CTNAG 4 cut(s) 316, 666, 929, 957
HpySE526I ACGT 2 cut(s) 338, 1067
Hsp92II CATG 5 cut(s) 98, 551, 555, 562, 921
HspAI GCGC 1 cut(s) 1112
Kzo9I GATC 4 cut(s) 28, 736, 863, 1011
LmnI GCTCC 4 cut(s) 305, 958, 964, 1340
Lsp1109I GCAGC 5 cut(s) 85, 202, 305, 841, 1140
LweI GCATC 6 cut(s) 96, 355, 447, 777, 1140, 1255
MaeI CTAG 1 cut(s) 731
MaeII ACGT 2 cut(s) 338, 1067
MaeIII GTNAC 5 cut(s) 140, 500, 514, 793, 846
MalI GATC 4 cut(s) 30, 738, 865, 1013
MboI GATC 4 cut(s) 28, 736, 863, 1011
MboII GAAGA 7 cut(s) 196, 199, 207, 440, 443, 673, 1197
MhlI GDGCHC 1 cut(s) 911
MluCI AATT 3 cut(s) 469, 933, 1006
MlyI GAGTC 1 cut(s) 1086
MmeI TCCRAC 3 cut(s) 724, 1138, 1240
MnlI CCTC 8 cut(s) 260, 267, 270, 287, 712, 966, 985, 1218
Mph1103I ATGCAT 2 cut(s) 553, 919
MseI TTAA 5 cut(s) 108, 578, 636, 725, 1019
MslI CAYNNNNRTG 3 cut(s) 56, 774, 1253
MspA1I CMGCKG 2 cut(s) 854, 860
MspCI CTTAAG 1 cut(s) 1018
MspI CCGG 3 cut(s) 9, 128, 412
MspR9I CCNGG 4 cut(s) 9, 232, 989, 1125
Mva1269I GAATGC 1 cut(s) 482
MvaI CCWGG 3 cut(s) 232, 989, 1125
MwoI GCNNNNNNNGC 4 cut(s) 196, 299, 365, 1029
NciI CCSGG 1 cut(s) 9
NdeI CATATG 1 cut(s) 754
NdeII GATC 4 cut(s) 28, 736, 863, 1011
NlaIII CATG 5 cut(s) 98, 551, 555, 562, 921
NlaIV GGNNCC 4 cut(s) 6, 229, 696, 760
NmuCI GTSAC 2 cut(s) 500, 846
NsiI ATGCAT 2 cut(s) 553, 919
NspV TTCGAA 1 cut(s) 474
PagI TCATGA 1 cut(s) 558
PctI GAATGC 1 cut(s) 482
PfeI GAWTC 2 cut(s) 652, 1098
PfoI TCCNGGA 1 cut(s) 987
PkrI GCNGC 6 cut(s) 100, 192, 295, 856, 859, 1130
PleI GAGTC 1 cut(s) 1085
PpsI GAGTC 1 cut(s) 1085
PpuMI RGGWCCY 1 cut(s) 695
Psp1406I AACGTT 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 695
Psp6I CCWGG 3 cut(s) 230, 987, 1123
PspFI CCCAGC 1 cut(s) 1304
PspGI CCWGG 3 cut(s) 230, 987, 1123
PspN4I GGNNCC 4 cut(s) 6, 229, 696, 760
PspPI GGNCC 4 cut(s) 4, 228, 394, 695
PspPPI RGGWCCY 1 cut(s) 695
PstI CTGCAG 2 cut(s) 123, 304
PstNI CAGNNNCTG 2 cut(s) 299, 1124
PvuII CAGCTG 1 cut(s) 854
RsaI GTAC 3 cut(s) 626, 1279, 1293
RsaNI GTAC 3 cut(s) 625, 1278, 1292
RseI CAYNNNNRTG 3 cut(s) 56, 774, 1253
SaqAI TTAA 5 cut(s) 108, 578, 636, 725, 1019
SatI GCNGC 6 cut(s) 99, 191, 294, 855, 858, 1129
Sau3AI GATC 4 cut(s) 28, 736, 863, 1011
Sau96I GGNCC 4 cut(s) 4, 228, 394, 695
SchI GAGTC 1 cut(s) 1086
ScrFI CCNGG 4 cut(s) 9, 232, 989, 1125
SduI GDGCHC 1 cut(s) 911
SfaNI GCATC 6 cut(s) 96, 355, 447, 777, 1140, 1255
SfcI CTRYAG 2 cut(s) 119, 300
SfuI TTCGAA 1 cut(s) 474
SinI GGWCC 4 cut(s) 4, 228, 394, 695
SmiMI CAYNNNNRTG 3 cut(s) 56, 774, 1253
SmlI CTYRAG 3 cut(s) 167, 1018, 1330
SmoI CTYRAG 3 cut(s) 167, 1018, 1330
Sse9I AATT 3 cut(s) 469, 933, 1006
SsiI CCGC 4 cut(s) 386, 426, 436, 858
SspMI CTAG 1 cut(s) 731
StyD4I CCNGG 4 cut(s) 7, 230, 987, 1123
StyI CCWWGG 3 cut(s) 24, 730, 940
TaaI ACNGT 6 cut(s) 146, 289, 508, 799, 1141, 1282
TaiI ACGT 2 cut(s) 341, 1070
TaqI TCGA 7 cut(s) 246, 333, 372, 474, 1010, 1055, 1322
TasI AATT 3 cut(s) 469, 933, 1006
TatI WGTACW 1 cut(s) 624
TauI GCSGC 1 cut(s) 860
TfiI GAWTC 2 cut(s) 652, 1098
Tru1I TTAA 5 cut(s) 108, 578, 636, 725, 1019
Tru9I TTAA 5 cut(s) 108, 578, 636, 725, 1019
TscAI CASTG 4 cut(s) 151, 292, 781, 1287
TseFI GTSAC 2 cut(s) 500, 846
TseI GCWGC 5 cut(s) 98, 190, 293, 854, 1128
Tsp45I GTSAC 2 cut(s) 500, 846
TspDTI ATGAA 6 cut(s) 5, 58, 83, 501, 575, 990
TspRI CASTG 4 cut(s) 151, 292, 781, 1287
Vha464I CTTAAG 1 cut(s) 1018
VpaK11BI GGWCC 4 cut(s) 4, 228, 394, 695
XapI RAATTY 2 cut(s) 933, 1006
XmaJI CCTAGG 1 cut(s) 730
XspI CTAG 1 cut(s) 731
Zsp2I ATGCAT 2 cut(s) 553, 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.