Rmu_sc0001935.1_g000003

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001935.1
OG0018216
Physical Location & Seq
Reverse (-)
7749 .. 11773
4025 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1032 bp
atgtctggcgtatcggttgcagtggctcctaggtcggagcctgataaaactacaactgctggatcacaacaccaggaaaagccacatcgccaactgcaccaacagcggcagcagccagctgttggaggtgtcatggggtcattgcgcgtaatagagctccagctcgtggcattcatcatggtgttctctgccagtggcctcgtcccgctccttgatttgatcttccctgcttttgcctccgcctatctcttagtactgtcgcgcttggccttcccatcacatggtcatgtctcttctggcgcgcaggagattttccaaggaagcaaattctttaggctgtatgtgattgtgggaactacaattgggctgttcttgcctttggcctatgtgttgggtggttttgcaaggggtgatgagcacgctgtgcgctcagcaacacctcacttgttcttgctctcctttcagattctgactgaaaatgtcataagtgggttgtcactattttcgccaccagttagagcacttgttccgttgctttacacagttaggagaatctttgtcatccttgattggatgaaggatgtctggcttaacaagactctccctgcaaatgcacaattgaaggtacttaatcccacactagctgcatttagtcttttctacttgtttttggttactatggtcttagtctctccaagagccaattgtcagcgtcaccagatcaacccggcgaccgtacttccagtcctagtctctccaagagccgactgccagtaccactgccaccgagactctctcaaccagcgaagcaagggcaagaattcctttctcggaaggctagagaagaaccttgtggcgaggttggtgctctcctcgtccacaacgcttgaagaagaagtcaggtcaagggactcccccgacgactgcaccccacggtgctggcatgcccgcacacgcgctcaaaatagacagtttgcatgccaactggttttggagccaaacattttggcacgcccagtgggacctcaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

343

Amino Acids

38.04

Weight (kDa)

9.88

Isoelectric Point (pI)

54.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7733 PF24867 49 - 221 8.3e-61 Domain of unknown function (DUF7733)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 122, 166, 281
AccBSI CCGCTC 1 cut(s) 208
AccII CGCG 4 cut(s) 147, 262, 302, 957
AciI CCGC 4 cut(s) 106, 206, 240, 949
AclWI GGATC 1 cut(s) 70
AcsI RAATTY 2 cut(s) 326, 820
AdeI CACNNNGTG 1 cut(s) 424
AfaI GTAC 4 cut(s) 255, 627, 738, 776
AfiI CCNNNNNNNGG 3 cut(s) 122, 166, 281
AgsI TTSAA 2 cut(s) 622, 890
AjnI CCWGG 1 cut(s) 72
AluBI AGCT 4 cut(s) 119, 157, 163, 644
AluI AGCT 4 cut(s) 119, 157, 163, 644
Alw21I GWGCWC 4 cut(s) 159, 420, 523, 870
Alw26I GTCTC 4 cut(s) 295, 694, 757, 783
AlwI GGATC 1 cut(s) 70
AlwNI CAGNNNCTG 1 cut(s) 469
AoxI GGCC 3 cut(s) 196, 267, 381
ApeKI GCWGC 3 cut(s) 109, 112, 644
ApoI RAATTY 2 cut(s) 326, 820
AspA2I CCTAGG 1 cut(s) 29
AspLEI GCGC 6 cut(s) 147, 264, 302, 304, 429, 959
AspS9I GGNCC 1 cut(s) 1022
AsuC2I CCSGG 1 cut(s) 728
AsuHPI GGTGA 2 cut(s) 422, 707
AvaII GGWCC 1 cut(s) 1022
AvrII CCTAGG 1 cut(s) 29
BanII GRGCYC 1 cut(s) 159
BauI CACGAG 1 cut(s) 164
Bbv12I GWGCWC 4 cut(s) 159, 420, 523, 870
BbvI GCAGC 3 cut(s) 121, 124, 631
BccI CCATC 1 cut(s) 283
BcgI CGANNNNNNTGC 2 cut(s) 847, 881
BciT130I CCWGG 1 cut(s) 74
BcnI CCSGG 1 cut(s) 728
BcoDI GTCTC 4 cut(s) 295, 694, 757, 783
BfaI CTAG 4 cut(s) 30, 641, 749, 839
BisI GCNGC 4 cut(s) 107, 110, 113, 645
BlnI CCTAGG 1 cut(s) 29
BlpI GCTNAGC 1 cut(s) 430
BlsI GCNGC 4 cut(s) 108, 111, 114, 646
BmcAI AGTACT 1 cut(s) 255
Bme1390I CCNGG 2 cut(s) 74, 728
Bme18I GGWCC 1 cut(s) 1022
BmgT120I GGNCC 1 cut(s) 1022
BmiI GGNNCC 4 cut(s) 27, 39, 996, 1023
BmrFI CCNGG 2 cut(s) 74, 728
BmrI ACTGGG 1 cut(s) 1010
BmuI ACTGGG 1 cut(s) 1010
BplI GAGNNNNNCTC 2 cut(s) 780, 812
BpmI CTGGAG 1 cut(s) 143
Bpu1102I GCTNAGC 1 cut(s) 430
BpuMI CCSGG 1 cut(s) 728
BsaJI CCNNGG 3 cut(s) 29, 316, 932
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 166, 281
Bse1I ACTGG 6 cut(s) 192, 512, 743, 772, 990, 1016
Bse3DI GCAATG 1 cut(s) 140
BseBI CCWGG 1 cut(s) 74
BseDI CCNNGG 3 cut(s) 29, 316, 932
BseGI GGATG 3 cut(s) 561, 579, 586
BseLI CCNNNNNNNGG 3 cut(s) 122, 166, 281
BseMI GCAATG 1 cut(s) 140
BseMII CTCAG 1 cut(s) 444
BseNI ACTGG 6 cut(s) 192, 512, 743, 772, 990, 1016
BsePI GCGCGC 1 cut(s) 300
BseRI GAGGAG 1 cut(s) 862
BseXI GCAGC 3 cut(s) 121, 124, 631
BsgI GTGCAG 2 cut(s) 80, 910
Bsh1236I CGCG 4 cut(s) 147, 262, 302, 957
Bsh1285I CGRYCG 1 cut(s) 735
BshFI GGCC 3 cut(s) 198, 269, 383
BsiEI CGRYCG 1 cut(s) 735
BsiHKAI GWGCWC 4 cut(s) 159, 420, 523, 870
BsiSI CCGG 1 cut(s) 728
BslFI GGGAC 2 cut(s) 188, 923
BslI CCNNNNNNNGG 3 cut(s) 122, 166, 281
BsmAI GTCTC 4 cut(s) 295, 694, 757, 783
BsmFI GGGAC 2 cut(s) 188, 923
BsmI GAATGC 1 cut(s) 170
BsnI GGCC 3 cut(s) 198, 269, 383
Bsp1286I GDGCHC 4 cut(s) 159, 420, 523, 870
Bsp143I GATC 3 cut(s) 62, 219, 720
Bsp1720I GCTNAGC 1 cut(s) 430
BspACI CCGC 4 cut(s) 106, 206, 240, 949
BspANI GGCC 3 cut(s) 198, 269, 383
BspCNI CTCAG 1 cut(s) 443
BspFNI CGCG 4 cut(s) 147, 262, 302, 957
BspLI GGNNCC 4 cut(s) 27, 39, 996, 1023
BspPI GGATC 1 cut(s) 70
BsrBI CCGCTC 1 cut(s) 208
BsrDI GCAATG 1 cut(s) 140
BsrI ACTGG 6 cut(s) 192, 512, 743, 772, 990, 1016
BssECI CCNNGG 3 cut(s) 29, 316, 932
BssHII GCGCGC 1 cut(s) 300
BssMI GATC 3 cut(s) 62, 219, 720
BssSI CACGAG 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 29, 316
Bst2BI CACGAG 1 cut(s) 164
Bst2UI CCWGG 1 cut(s) 74
Bst4CI ACNGT 5 cut(s) 258, 544, 736, 936, 972
Bst6I CTCTTC 1 cut(s) 298
BstAPI GCANNNNNTGC 1 cut(s) 424
BstC8I GCNNGC 8 cut(s) 117, 302, 420, 941, 945, 949, 979, 1012
BstDEI CTNAG 3 cut(s) 250, 430, 685
BstDSI CCRYGG 1 cut(s) 932
BstF5I GGATG 3 cut(s) 561, 579, 586
BstFNI CGCG 4 cut(s) 147, 262, 302, 957
BstHHI GCGC 6 cut(s) 147, 264, 302, 304, 429, 959
BstKTI GATC 3 cut(s) 65, 222, 723
BstMAI GTCTC 4 cut(s) 295, 694, 757, 783
BstMBI GATC 3 cut(s) 62, 219, 720
BstMCI CGRYCG 1 cut(s) 735
BstMWI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
BstNI CCWGG 1 cut(s) 74
BstNSI RCATGY 2 cut(s) 947, 981
BstSCI CCNGG 2 cut(s) 72, 726
BstUI CGCG 4 cut(s) 147, 262, 302, 957
BstV1I GCAGC 3 cut(s) 121, 124, 631
BstXI CCANNNNNNTGG 1 cut(s) 939
BsuRI GGCC 3 cut(s) 198, 269, 383
BtgI CCRYGG 1 cut(s) 932
BtgZI GCGATG 1 cut(s) 71
BtsCI GGATG 3 cut(s) 561, 579, 586
BtsI GCAGTG 2 cut(s) 27, 778
BtsIMutI CAGTG 4 cut(s) 27, 199, 778, 1023
Cac8I GCNNGC 8 cut(s) 117, 302, 420, 941, 945, 949, 979, 1012
CaiI CAGNNNCTG 1 cut(s) 469
CfoI GCGC 6 cut(s) 147, 264, 302, 304, 429, 959
Cfr13I GGNCC 1 cut(s) 1022
CseI GACGC 1 cut(s) 701
Csp6I GTAC 4 cut(s) 254, 626, 737, 775
CspCI CAANNNNNGTGG 2 cut(s) 868, 903
CviAII CATG 6 cut(s) 133, 178, 281, 287, 944, 978
CviQI GTAC 4 cut(s) 254, 626, 737, 775
DdeI CTNAG 3 cut(s) 250, 430, 685
DpnI GATC 3 cut(s) 64, 221, 722
DpnII GATC 3 cut(s) 62, 219, 720
DraIII CACNNNGTG 1 cut(s) 424
Eam1104I CTCTTC 1 cut(s) 298
EarI CTCTTC 1 cut(s) 298
EciI GGCGGA 1 cut(s) 229
Ecl136II GAGCTC 1 cut(s) 157
Eco130I CCWWGG 2 cut(s) 29, 316
Eco24I GRGCYC 1 cut(s) 159
Eco47I GGWCC 1 cut(s) 1022
Eco53kI GAGCTC 1 cut(s) 157
EcoICRI GAGCTC 1 cut(s) 157
EcoO109I RGGNCCY 1 cut(s) 1022
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 1 cut(s) 72
EcoT14I CCWWGG 2 cut(s) 29, 316
EcoT38I GRGCYC 1 cut(s) 159
ErhI CCWWGG 2 cut(s) 29, 316
FaeI CATG 6 cut(s) 136, 181, 284, 290, 947, 981
FalI AAGNNNNNCTT 2 cut(s) 809, 841
FaqI GGGAC 2 cut(s) 188, 923
FatI CATG 6 cut(s) 132, 177, 280, 286, 943, 977
FauI CCCGC 2 cut(s) 213, 956
Fnu4HI GCNGC 4 cut(s) 107, 110, 113, 645
FokI GGATG 3 cut(s) 548, 586, 593
FriOI GRGCYC 1 cut(s) 159
Fsp4HI GCNGC 4 cut(s) 107, 110, 113, 645
FspBI CTAG 4 cut(s) 30, 641, 749, 839
GlaI GCGC 6 cut(s) 146, 263, 301, 303, 428, 958
GluI GCNGC 4 cut(s) 107, 110, 113, 645
GsuI CTGGAG 1 cut(s) 143
HaeIII GGCC 3 cut(s) 198, 269, 383
HapII CCGG 1 cut(s) 728
HgaI GACGC 1 cut(s) 701
HhaI GCGC 6 cut(s) 147, 264, 302, 304, 429, 959
Hin1II CATG 6 cut(s) 136, 181, 284, 290, 947, 981
Hin6I GCGC 6 cut(s) 145, 262, 300, 302, 427, 957
HinP1I GCGC 6 cut(s) 145, 262, 300, 302, 427, 957
HinfI GANTC 5 cut(s) 466, 552, 598, 791, 911
HpaII CCGG 1 cut(s) 728
HphI GGTGA 2 cut(s) 422, 707
Hpy166II GTNNAC 1 cut(s) 879
Hpy188I TCNGA 4 cut(s) 37, 465, 471, 833
Hpy8I GTNNAC 1 cut(s) 879
Hpy99I CGWCG 1 cut(s) 923
HpyAV CCTTC 4 cut(s) 280, 571, 616, 828
HpyCH4III ACNGT 5 cut(s) 258, 544, 736, 936, 972
HpyCH4V TGCA 8 cut(s) 20, 97, 404, 608, 614, 647, 927, 977
HpyF10VI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
HpyF3I CTNAG 3 cut(s) 250, 430, 685
Hsp92II CATG 6 cut(s) 136, 181, 284, 290, 947, 981
HspAI GCGC 6 cut(s) 145, 262, 300, 302, 427, 957
Kzo9I GATC 3 cut(s) 62, 219, 720
LmnI GCTCC 5 cut(s) 31, 37, 162, 213, 994
Lsp1109I GCAGC 3 cut(s) 121, 124, 631
MaeI CTAG 4 cut(s) 30, 641, 749, 839
MaeIII GTNAC 3 cut(s) 495, 673, 713
MalI GATC 3 cut(s) 64, 221, 722
MbiI CCGCTC 1 cut(s) 208
MboI GATC 3 cut(s) 62, 219, 720
MboII GAAGA 5 cut(s) 214, 285, 856, 902, 905
MfeI CAATTG 3 cut(s) 360, 617, 703
MhlI GDGCHC 4 cut(s) 159, 420, 523, 870
MluCI AATT 5 cut(s) 326, 360, 617, 703, 820
MlyI GAGTC 3 cut(s) 592, 785, 905
MmeI TCCRAC 2 cut(s) 15, 103
MnlI CCTC 6 cut(s) 119, 209, 247, 450, 852, 883
MseI TTAA 2 cut(s) 591, 630
MslI CAYNNNNRTG 2 cut(s) 179, 285
MspA1I CMGCKG 2 cut(s) 106, 119
MspI CCGG 1 cut(s) 728
MspR9I CCNGG 2 cut(s) 74, 728
MunI CAATTG 3 cut(s) 360, 617, 703
Mva1269I GAATGC 1 cut(s) 170
MvaI CCWGG 1 cut(s) 74
MvnI CGCG 4 cut(s) 147, 262, 302, 957
MwoI GCNNNNNNNGC 4 cut(s) 103, 112, 373, 424
NciI CCSGG 1 cut(s) 728
NdeII GATC 3 cut(s) 62, 219, 720
NlaIII CATG 6 cut(s) 136, 181, 284, 290, 947, 981
NlaIV GGNNCC 4 cut(s) 27, 39, 996, 1023
NmuCI GTSAC 2 cut(s) 495, 713
NspI RCATGY 2 cut(s) 947, 981
PaeI GCATGC 2 cut(s) 947, 981
PauI GCGCGC 1 cut(s) 300
PctI GAATGC 1 cut(s) 170
PfeI GAWTC 2 cut(s) 466, 552
PflMI CCANNNNNTGG 3 cut(s) 122, 166, 281
PkrI GCNGC 4 cut(s) 108, 111, 114, 646
PleI GAGTC 3 cut(s) 592, 785, 905
PpsI GAGTC 3 cut(s) 592, 785, 905
PpuMI RGGWCCY 1 cut(s) 1022
Psp124BI GAGCTC 1 cut(s) 159
Psp5II RGGWCCY 1 cut(s) 1022
Psp6I CCWGG 1 cut(s) 72
PspGI CCWGG 1 cut(s) 72
PspN4I GGNNCC 4 cut(s) 27, 39, 996, 1023
PspPI GGNCC 1 cut(s) 1022
PspPPI RGGWCCY 1 cut(s) 1022
PstNI CAGNNNCTG 1 cut(s) 469
PteI GCGCGC 1 cut(s) 300
PvuII CAGCTG 1 cut(s) 119
RsaI GTAC 4 cut(s) 255, 627, 738, 776
RsaNI GTAC 4 cut(s) 254, 626, 737, 775
RseI CAYNNNNRTG 2 cut(s) 179, 285
SacI GAGCTC 1 cut(s) 159
SaqAI TTAA 2 cut(s) 591, 630
SatI GCNGC 4 cut(s) 107, 110, 113, 645
Sau3AI GATC 3 cut(s) 62, 219, 720
Sau96I GGNCC 1 cut(s) 1022
ScaI AGTACT 1 cut(s) 255
SchI GAGTC 3 cut(s) 592, 785, 905
ScrFI CCNGG 2 cut(s) 74, 728
SduI GDGCHC 4 cut(s) 159, 420, 523, 870
SinI GGWCC 1 cut(s) 1022
SmiMI CAYNNNNRTG 2 cut(s) 179, 285
SphI GCATGC 2 cut(s) 947, 981
Sse9I AATT 5 cut(s) 326, 360, 617, 703, 820
SsiI CCGC 4 cut(s) 106, 206, 240, 949
SspMI CTAG 4 cut(s) 30, 641, 749, 839
SstI GAGCTC 1 cut(s) 159
StyD4I CCNGG 2 cut(s) 72, 726
StyI CCWWGG 2 cut(s) 29, 316
TaaI ACNGT 5 cut(s) 258, 544, 736, 936, 972
TasI AATT 5 cut(s) 326, 360, 617, 703, 820
TatI WGTACW 1 cut(s) 253
TauI GCSGC 1 cut(s) 109
TfiI GAWTC 2 cut(s) 466, 552
Tru1I TTAA 2 cut(s) 591, 630
Tru9I TTAA 2 cut(s) 591, 630
TscAI CASTG 4 cut(s) 27, 199, 785, 1023
TseFI GTSAC 2 cut(s) 495, 713
TseI GCWGC 3 cut(s) 109, 112, 644
Tsp45I GTSAC 2 cut(s) 495, 713
TspDTI ATGAA 2 cut(s) 163, 590
TspGWI ACGGA 1 cut(s) 519
TspRI CASTG 4 cut(s) 27, 199, 785, 1023
Van91I CCANNNNNTGG 3 cut(s) 122, 166, 281
VpaK11BI GGWCC 1 cut(s) 1022
XapI RAATTY 2 cut(s) 326, 820
XceI RCATGY 2 cut(s) 947, 981
XmaJI CCTAGG 1 cut(s) 29
XspI CTAG 4 cut(s) 30, 641, 749, 839
ZrmI AGTACT 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.