Rh3AG281000

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0018216
Physical Location & Seq
Forward (+)
32766870 .. 32781709
14840 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG281000.1

Sequence Viewer

Length: 441 bp
ATGGATAAGGTGGAGGAGTGGCGAAGAACACTCAGTGATGTTGCAGACATGGGAGGGATGGTTTTAGGAAATCAGTATGAGTCACAGTTCATCCAACATATTGTTCATGAAACTCTGAAAATGCTGAATGAATCGTTATTTAAAGCTACCCAACGAAATCAGATCGATCCTAAGCTTCGTGGTGTCGCCGACAGATTCCCAGCCAATAGCGCCGGCGAGGTAGGTGTAGTCAGTGTAGAAGGTGAGCTTCTTGTCCCGGGAGCAGAGCTAGCGGCAGGCCTCCTCGACAAAGAGGAACTCCGGCGAGGTGAGGAGCTGGTTAGGATTTTGAATTGGATCCTAGAGATCCTGCAACAGGCTATAGAGGTGGGTCGGAGTTATTGTGGTCGTGATCTGAGGGGGAAGCCGAGACGGATAGGAGTTATTGTGGTCGTGATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

146

Amino Acids

16.36

Weight (kDa)

5.49

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 272
AclWI GGATC 4 cut(s) 161, 331, 340, 344
AdeI CACNNNGTG 1 cut(s) 35
AfiI CCNNNNNNNGG 1 cut(s) 355
AgsI TTSAA 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 87, 119
AluBI AGCT 5 cut(s) 146, 175, 247, 268, 316
AluI AGCT 5 cut(s) 146, 175, 247, 268, 316
Alw26I GTCTC 1 cut(s) 403
AlwI GGATC 4 cut(s) 161, 331, 340, 344
Ama87I CYCGRG 1 cut(s) 256
AoxI GGCC 1 cut(s) 277
AspLEI GCGC 1 cut(s) 212
AsuC2I CCSGG 2 cut(s) 257, 258
AsuHPI GGTGA 2 cut(s) 254, 320
AsuNHI GCTAGC 1 cut(s) 268
AvaI CYCGRG 1 cut(s) 256
BamHI GGATCC 1 cut(s) 336
BccI CCATC 1 cut(s) 52
BcnI CCSGG 2 cut(s) 257, 258
BcoDI GTCTC 1 cut(s) 403
BfaI CTAG 2 cut(s) 269, 341
BfmI CTRYAG 1 cut(s) 360
BfoI RGCGCY 1 cut(s) 213
BisI GCNGC 1 cut(s) 273
BlsI GCNGC 1 cut(s) 274
Bme1390I CCNGG 2 cut(s) 257, 258
BmeT110I CYCGRG 1 cut(s) 256
BmiI GGNNCC 1 cut(s) 338
BmrFI CCNGG 2 cut(s) 257, 258
BmtI GCTAGC 1 cut(s) 272
Bpu10I CCTNAGC 1 cut(s) 171
BpuMI CCSGG 2 cut(s) 257, 258
Bsa29I ATCGAT 1 cut(s) 165
BsaJI CCNNGG 1 cut(s) 256
BsaXI ACNNNNNCTCC 6 cut(s) 45, 75, 367, 397, 411, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 355
Bse118I RCCGGY 1 cut(s) 212
BseCI ATCGAT 1 cut(s) 165
BseDI CCNNGG 1 cut(s) 256
BseGI GGATG 2 cut(s) 63, 90
BseLI CCNNNNNNNGG 1 cut(s) 355
BseMII CTCAG 2 cut(s) 46, 386
BseRI GAGGAG 3 cut(s) 29, 272, 326
BseYI CCCAGC 1 cut(s) 199
BshFI GGCC 1 cut(s) 279
BshVI ATCGAT 1 cut(s) 165
BsiHKCI CYCGRG 1 cut(s) 256
BsiSI CCGG 3 cut(s) 213, 257, 301
BslFI GGGAC 1 cut(s) 239
BslI CCNNNNNNNGG 1 cut(s) 355
BsmAI GTCTC 1 cut(s) 403
BsmBI CGTCTC 1 cut(s) 403
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 1 cut(s) 279
BsoBI CYCGRG 1 cut(s) 256
Bsp143I GATC 6 cut(s) 162, 166, 336, 345, 391, 435
BspACI CCGC 1 cut(s) 272
BspANI GGCC 1 cut(s) 279
BspCNI CTCAG 2 cut(s) 45, 387
BspDI ATCGAT 1 cut(s) 165
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 338
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 4 cut(s) 161, 331, 340, 344
BsrFI RCCGGY 1 cut(s) 212
BssAI RCCGGY 1 cut(s) 212
BssECI CCNNGG 1 cut(s) 256
BssMI GATC 6 cut(s) 162, 166, 336, 345, 391, 435
Bst4CI ACNGT 1 cut(s) 87
BstC8I GCNNGC 3 cut(s) 214, 270, 277
BstDEI CTNAG 3 cut(s) 32, 171, 395
BstENI CCTNNNNNAGG 1 cut(s) 353
BstF5I GGATG 2 cut(s) 63, 90
BstH2I RGCGCY 1 cut(s) 213
BstHHI GCGC 1 cut(s) 212
BstKTI GATC 6 cut(s) 165, 169, 339, 348, 394, 438
BstMAI GTCTC 1 cut(s) 403
BstMBI GATC 6 cut(s) 162, 166, 336, 345, 391, 435
BstMWI GCNNNNNNNGC 2 cut(s) 209, 269
BstSCI CCNGG 2 cut(s) 255, 256
BstSFI CTRYAG 1 cut(s) 360
BstX2I RGATCY 2 cut(s) 336, 345
BstYI RGATCY 2 cut(s) 336, 345
Bsu15I ATCGAT 1 cut(s) 165
BsuRI GGCC 1 cut(s) 279
BsuTUI ATCGAT 1 cut(s) 165
BtsCI GGATG 2 cut(s) 63, 90
BtsIMutI CAGTG 2 cut(s) 40, 238
Cac8I GCNNGC 3 cut(s) 214, 270, 277
CciI TCATGA 1 cut(s) 106
CfoI GCGC 1 cut(s) 212
Cfr10I RCCGGY 1 cut(s) 212
Cfr9I CCCGGG 1 cut(s) 256
ClaI ATCGAT 1 cut(s) 165
CviAII CATG 2 cut(s) 49, 107
CviJI RGCY 9 cut(s) 146, 175, 203, 247, 268, 279, 316, 359, 406
CviKI_1 RGCY 9 cut(s) 146, 175, 203, 247, 268, 279, 316, 359, 406
DdeI CTNAG 3 cut(s) 32, 171, 395
DpnI GATC 6 cut(s) 164, 168, 338, 347, 393, 437
DpnII GATC 6 cut(s) 162, 166, 336, 345, 391, 435
DraI TTTAAA 1 cut(s) 142
DraIII CACNNNGTG 1 cut(s) 35
Eco147I AGGCCT 1 cut(s) 279
Eco88I CYCGRG 1 cut(s) 256
EcoNI CCTNNNNNAGG 1 cut(s) 353
Esp3I CGTCTC 1 cut(s) 403
FaeI CATG 2 cut(s) 52, 110
FaiI YATR 5 cut(s) 50, 78, 99, 108, 362
FalI AAGNNNNNCTT 2 cut(s) 231, 263
FaqI GGGAC 1 cut(s) 239
FatI CATG 2 cut(s) 48, 106
Fnu4HI GCNGC 1 cut(s) 273
FokI GGATG 2 cut(s) 70, 77
Fsp4HI GCNGC 1 cut(s) 273
FspBI CTAG 2 cut(s) 269, 341
GlaI GCGC 1 cut(s) 211
GluI GCNGC 1 cut(s) 273
GsaI CCCAGC 1 cut(s) 203
HaeII RGCGCY 1 cut(s) 213
HaeIII GGCC 1 cut(s) 279
HapII CCGG 3 cut(s) 213, 257, 301
HhaI GCGC 1 cut(s) 212
Hin1II CATG 2 cut(s) 52, 110
Hin6I GCGC 1 cut(s) 210
HinP1I GCGC 1 cut(s) 210
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 3 cut(s) 80, 131, 195
HpaII CCGG 3 cut(s) 213, 257, 301
HphI GGTGA 2 cut(s) 254, 320
Hpy188I TCNGA 5 cut(s) 117, 162, 375, 396, 440
Hpy188III TCNNGA 3 cut(s) 107, 389, 433
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 1 cut(s) 87
HpyCH4V TGCA 2 cut(s) 44, 352
HpyF10VI GCNNNNNNNGC 2 cut(s) 209, 269
HpyF3I CTNAG 3 cut(s) 32, 171, 395
Hsp92II CATG 2 cut(s) 52, 110
HspAI GCGC 1 cut(s) 210
KroI GCCGGC 1 cut(s) 212
KroNI GCCGGC 1 cut(s) 214
Kzo9I GATC 6 cut(s) 162, 166, 336, 345, 391, 435
LmnI GCTCC 2 cut(s) 260, 313
LpnPI CCDG 8 cut(s) 213, 226, 261, 270, 302, 314, 341, 362
MaeI CTAG 2 cut(s) 269, 341
MaeIII GTNAC 1 cut(s) 81
MalI GATC 6 cut(s) 164, 168, 338, 347, 393, 437
MboI GATC 6 cut(s) 162, 166, 336, 345, 391, 435
MboII GAAGA 1 cut(s) 36
MflI RGATCY 2 cut(s) 336, 345
MluCI AATT 1 cut(s) 331
MlyI GAGTC 1 cut(s) 89
MmeI TCCRAC 2 cut(s) 118, 353
MreI CGCCGGCG 1 cut(s) 212
MroNI GCCGGC 1 cut(s) 212
MseI TTAA 1 cut(s) 141
MspI CCGG 3 cut(s) 213, 257, 301
MspR9I CCNGG 2 cut(s) 257, 258
MwoI GCNNNNNNNGC 2 cut(s) 209, 269
NaeI GCCGGC 1 cut(s) 214
NciI CCSGG 2 cut(s) 257, 258
NdeII GATC 6 cut(s) 162, 166, 336, 345, 391, 435
NgoMIV GCCGGC 1 cut(s) 212
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 2 cut(s) 52, 110
NlaIV GGNNCC 1 cut(s) 338
NmeAIII GCCGAG 1 cut(s) 432
NmuCI GTSAC 1 cut(s) 81
PagI TCATGA 1 cut(s) 106
PceI AGGCCT 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 131, 195
PkrI GCNGC 1 cut(s) 274
PleI GAGTC 1 cut(s) 88
PpsI GAGTC 1 cut(s) 88
PspFI CCCAGC 1 cut(s) 199
PspN4I GGNNCC 1 cut(s) 338
PsuI RGATCY 2 cut(s) 336, 345
SaqAI TTAA 1 cut(s) 141
SatI GCNGC 1 cut(s) 273
Sau3AI GATC 6 cut(s) 162, 166, 336, 345, 391, 435
SchI GAGTC 1 cut(s) 89
ScrFI CCNGG 2 cut(s) 257, 258
SfcI CTRYAG 1 cut(s) 360
SgrAI CRCCGGYG 1 cut(s) 212
SmaI CCCGGG 1 cut(s) 258
Sse9I AATT 1 cut(s) 331
SseBI AGGCCT 1 cut(s) 279
SsiI CCGC 1 cut(s) 272
SspMI CTAG 2 cut(s) 269, 341
StuI AGGCCT 1 cut(s) 279
StyD4I CCNGG 2 cut(s) 255, 256
TaaI ACNGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 165, 285
TasI AATT 1 cut(s) 331
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 2 cut(s) 131, 195
Tru1I TTAA 1 cut(s) 141
Tru9I TTAA 1 cut(s) 141
TscAI CASTG 2 cut(s) 40, 238
TseFI GTSAC 1 cut(s) 81
Tsp45I GTSAC 1 cut(s) 81
TspDTI ATGAA 4 cut(s) 79, 95, 123, 144
TspGWI ACGGA 1 cut(s) 427
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 2 cut(s) 40, 238
XagI CCTNNNNNAGG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 256
XspI CTAG 2 cut(s) 269, 341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.