Rmu_sc0001955.1_g000030

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001955.1
OG0018216
Physical Location & Seq
Reverse (-)
121351 .. 122810
1460 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 939 bp
atgcaacatcacagagcttatggacaccaggttttgccagttttctatgatgtcgatccaaaggatgtgaggaatcagagagggagttttgcaaaagcatttgccaaacatgtattcgagcaaaaaatggataaggtggaggagtggcgaagagcactcagtgatgttgcagacatgggagggatggttttaggagatcagtatgagtcacagttcatccaacatattgttcatgaaactctgaaaatgctgaatgactcgttatttaaaggttgttccaatattgttggaatgccctaccgtgtgcaaacgctgaatagatggctgaaagatggatcaaatgatgttgatgtggctgtgatatgtggctgtttgagcccaaaagtaattttggcaagatcctttagtggatttagcacagcgggccgatacctgcgagccaaaaataagcctacttgggtttgggttacagcttcgcccattccgtggacaaagaaaggacctctctcaaatcaatcaatcccaacgattacaaagcctccccggagcaaaccttcaacctcgttgaaacccggtgaccgcgcgccagtcctagtctctccaagagccgattgtcagcatcaccagatcaatccggcgaccgtacttccagtcctagtatctccaagagctgactgccagtaccactgccaccgagactctatcaaccagcgaagcaagggcaggaagtcctttctcggaaggctggagaagaaccttgtggcgaggttggtgctctcctcgtccacaatgcttgaagaagaagtcaggtcaagggattccctcgacgactgcaccccacggtgctggcacgcccgcgtacacgctcaaaagagacagcttgcacgccaactggttttggagccaaacattttggcacgcccagtgggacgacaatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 NB-ARC TIR

Protein Analysis

312

Amino Acids

35.33

Weight (kDa)

9.85

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 7 - 88 2.8e-16 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 441
AccB7I CCANNNNNTGG 1 cut(s) 486
AccII CGCG 3 cut(s) 582, 584, 858
AciI CCGC 3 cut(s) 422, 580, 856
AclWI GGATC 3 cut(s) 50, 343, 393
AdeI CACNNNGTG 1 cut(s) 161
AfaI GTAC 3 cut(s) 645, 683, 861
AfiI CCNNNNNNNGG 1 cut(s) 486
AflIII ACRYGT 1 cut(s) 109
AgsI TTSAA 3 cut(s) 558, 568, 797
AjnI CCWGG 1 cut(s) 27
AjuI GAANNNNNNNTTGG 4 cut(s) 98, 130, 213, 245
AluBI AGCT 4 cut(s) 17, 473, 671, 880
AluI AGCT 4 cut(s) 17, 473, 671, 880
Alw21I GWGCWC 2 cut(s) 157, 777
Alw26I GTCTC 3 cut(s) 601, 690, 868
AlwI GGATC 3 cut(s) 50, 343, 393
AoxI GGCC 1 cut(s) 424
AspLEI GCGC 2 cut(s) 584, 586
AspS9I GGNCC 2 cut(s) 424, 500
AsuC2I CCSGG 2 cut(s) 544, 573
AsuHPI GGTGA 2 cut(s) 587, 614
AvaII GGWCC 1 cut(s) 500
BanII GRGCYC 1 cut(s) 380
Bbv12I GWGCWC 2 cut(s) 157, 777
BccI CCATC 3 cut(s) 178, 315, 326
BcgI CGANNNNNNTGC 2 cut(s) 754, 788
BciT130I CCWGG 1 cut(s) 29
BcnI CCSGG 2 cut(s) 544, 573
BcoDI GTCTC 3 cut(s) 601, 690, 868
BfaI CTAG 2 cut(s) 593, 656
BfuAI ACCTGC 1 cut(s) 441
Bme1390I CCNGG 3 cut(s) 29, 544, 573
Bme18I GGWCC 1 cut(s) 500
BmgT120I GGNCC 2 cut(s) 424, 500
BmiI GGNNCC 1 cut(s) 903
BmrFI CCNGG 3 cut(s) 29, 544, 573
BmrI ACTGGG 1 cut(s) 917
BmsI GCATC 1 cut(s) 628
BmuI ACTGGG 1 cut(s) 917
BpmI CTGGAG 1 cut(s) 767
BpuMI CCSGG 2 cut(s) 544, 573
BsaBI GATNNNNATC 1 cut(s) 54
BsaJI CCNNGG 3 cut(s) 485, 542, 839
BsaXI ACNNNNNCTCC 4 cut(s) 171, 201, 523, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 486
Bse1I ACTGG 6 cut(s) 38, 587, 650, 679, 897, 923
Bse8I GATNNNNATC 1 cut(s) 54
BseBI CCWGG 1 cut(s) 29
BseDI CCNNGG 3 cut(s) 485, 542, 839
BseGI GGATG 3 cut(s) 70, 189, 216
BseJI GATNNNNATC 1 cut(s) 54
BseLI CCNNNNNNNGG 1 cut(s) 486
BseMII CTCAG 1 cut(s) 172
BseNI ACTGG 6 cut(s) 38, 587, 650, 679, 897, 923
BsePI GCGCGC 1 cut(s) 582
BseRI GAGGAG 2 cut(s) 155, 769
BsgI GTGCAG 1 cut(s) 817
Bsh1236I CGCG 3 cut(s) 582, 584, 858
Bsh1285I CGRYCG 1 cut(s) 642
BshFI GGCC 1 cut(s) 426
BsiEI CGRYCG 1 cut(s) 642
BsiHKAI GWGCWC 2 cut(s) 157, 777
BsiSI CCGG 3 cut(s) 544, 573, 635
BslI CCNNNNNNNGG 1 cut(s) 486
BsmAI GTCTC 3 cut(s) 601, 690, 868
BsmI GAATGC 1 cut(s) 297
BsnI GGCC 1 cut(s) 426
Bsp1286I GDGCHC 3 cut(s) 157, 380, 777
Bsp143I GATC 5 cut(s) 55, 196, 335, 398, 627
BspACI CCGC 3 cut(s) 422, 580, 856
BspANI GGCC 1 cut(s) 426
BspCNI CTCAG 1 cut(s) 171
BspFNI CGCG 3 cut(s) 582, 584, 858
BspHI TCATGA 1 cut(s) 232
BspLI GGNNCC 1 cut(s) 903
BspMI ACCTGC 1 cut(s) 441
BspPI GGATC 3 cut(s) 50, 343, 393
BspQI GCTCTTC 1 cut(s) 145
BsrI ACTGG 6 cut(s) 38, 587, 650, 679, 897, 923
BssECI CCNNGG 3 cut(s) 485, 542, 839
BssHII GCGCGC 1 cut(s) 582
BssMI GATC 5 cut(s) 55, 196, 335, 398, 627
Bst2UI CCWGG 1 cut(s) 29
Bst4CI ACNGT 4 cut(s) 213, 302, 643, 843
Bst6I CTCTTC 1 cut(s) 145
BstC8I GCNNGC 9 cut(s) 424, 438, 584, 848, 852, 856, 882, 886, 919
BstDEI CTNAG 1 cut(s) 158
BstDSI CCRYGG 2 cut(s) 485, 839
BstEII GGTNACC 1 cut(s) 575
BstF5I GGATG 3 cut(s) 70, 189, 216
BstFNI CGCG 3 cut(s) 582, 584, 858
BstHHI GCGC 2 cut(s) 584, 586
BstKTI GATC 5 cut(s) 58, 199, 338, 401, 630
BstMAI GTCTC 3 cut(s) 601, 690, 868
BstMBI GATC 5 cut(s) 55, 196, 335, 398, 627
BstMCI CGRYCG 1 cut(s) 642
BstMWI GCNNNNNNNGC 2 cut(s) 375, 423
BstNI CCWGG 1 cut(s) 29
BstNSI RCATGY 1 cut(s) 113
BstPI GGTNACC 1 cut(s) 575
BstSCI CCNGG 3 cut(s) 27, 542, 571
BstUI CGCG 3 cut(s) 582, 584, 858
BstX2I RGATCY 1 cut(s) 398
BstXI CCANNNNNNTGG 1 cut(s) 846
BstYI RGATCY 1 cut(s) 398
BsuRI GGCC 1 cut(s) 426
BtgI CCRYGG 2 cut(s) 485, 839
BtsCI GGATG 3 cut(s) 70, 189, 216
BtsI GCAGTG 1 cut(s) 685
BtsIMutI CAGTG 3 cut(s) 166, 685, 930
BveI ACCTGC 1 cut(s) 441
Cac8I GCNNGC 9 cut(s) 424, 438, 584, 848, 852, 856, 882, 886, 919
CciI TCATGA 1 cut(s) 232
CfoI GCGC 2 cut(s) 584, 586
Cfr13I GGNCC 2 cut(s) 424, 500
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 3 cut(s) 644, 682, 860
CspCI CAANNNNNGTGG 2 cut(s) 775, 810
CviAII CATG 3 cut(s) 110, 175, 233
CviQI GTAC 3 cut(s) 644, 682, 860
DdeI CTNAG 1 cut(s) 158
DpnI GATC 5 cut(s) 57, 198, 337, 400, 629
DpnII GATC 5 cut(s) 55, 196, 335, 398, 627
DraI TTTAAA 1 cut(s) 268
DraIII CACNNNGTG 1 cut(s) 161
Eam1104I CTCTTC 1 cut(s) 145
EarI CTCTTC 1 cut(s) 145
Eco24I GRGCYC 1 cut(s) 380
Eco47I GGWCC 1 cut(s) 500
Eco91I GGTNACC 1 cut(s) 575
EcoO109I RGGNCCY 1 cut(s) 500
EcoO65I GGTNACC 1 cut(s) 575
EcoRII CCWGG 1 cut(s) 27
EcoT38I GRGCYC 1 cut(s) 380
FaeI CATG 3 cut(s) 113, 178, 236
FaiI YATR 8 cut(s) 21, 48, 111, 176, 204, 225, 234, 364
FatI CATG 3 cut(s) 109, 174, 232
FauI CCCGC 2 cut(s) 415, 863
FokI GGATG 3 cut(s) 77, 196, 203
FriOI GRGCYC 1 cut(s) 380
FspBI CTAG 2 cut(s) 593, 656
GlaI GCGC 2 cut(s) 583, 585
GsuI CTGGAG 1 cut(s) 767
HaeIII GGCC 1 cut(s) 426
HapII CCGG 3 cut(s) 544, 573, 635
HhaI GCGC 2 cut(s) 584, 586
Hin1II CATG 3 cut(s) 113, 178, 236
Hin6I GCGC 2 cut(s) 582, 584
HinP1I GCGC 2 cut(s) 582, 584
HinfI GANTC 5 cut(s) 73, 206, 257, 698, 818
HpaII CCGG 3 cut(s) 544, 573, 635
HphI GGTGA 2 cut(s) 587, 614
Hpy166II GTNNAC 3 cut(s) 489, 786, 862
Hpy188I TCNGA 3 cut(s) 78, 243, 740
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 3 cut(s) 489, 786, 862
Hpy99I CGWCG 1 cut(s) 830
HpyAV CCTTC 2 cut(s) 564, 735
HpyCH4III ACNGT 4 cut(s) 213, 302, 643, 843
HpyCH4V TGCA 6 cut(s) 4, 92, 170, 307, 834, 884
HpyF10VI GCNNNNNNNGC 2 cut(s) 375, 423
HpyF3I CTNAG 1 cut(s) 158
Hsp92II CATG 3 cut(s) 113, 178, 236
HspAI GCGC 2 cut(s) 582, 584
Kzo9I GATC 5 cut(s) 55, 196, 335, 398, 627
LguI GCTCTTC 1 cut(s) 145
LmnI GCTCC 2 cut(s) 546, 901
LweI GCATC 1 cut(s) 628
MabI ACCWGGT 1 cut(s) 27
MaeI CTAG 2 cut(s) 593, 656
MaeIII GTNAC 3 cut(s) 207, 466, 575
MalI GATC 5 cut(s) 57, 198, 337, 400, 629
MboI GATC 5 cut(s) 55, 196, 335, 398, 627
MboII GAAGA 4 cut(s) 162, 763, 809, 812
MflI RGATCY 1 cut(s) 398
MhlI GDGCHC 3 cut(s) 157, 380, 777
MluCI AATT 1 cut(s) 387
MlyI GAGTC 3 cut(s) 215, 251, 692
MmeI TCCRAC 2 cut(s) 244, 268
MseI TTAA 1 cut(s) 267
MspA1I CMGCKG 1 cut(s) 422
MspI CCGG 3 cut(s) 544, 573, 635
MspR9I CCNGG 3 cut(s) 29, 544, 573
Mva1269I GAATGC 1 cut(s) 297
MvaI CCWGG 1 cut(s) 29
MvnI CGCG 3 cut(s) 582, 584, 858
MwoI GCNNNNNNNGC 2 cut(s) 375, 423
NciI CCSGG 2 cut(s) 544, 573
NdeII GATC 5 cut(s) 55, 196, 335, 398, 627
NlaIII CATG 3 cut(s) 113, 178, 236
NlaIV GGNNCC 1 cut(s) 903
NmuCI GTSAC 2 cut(s) 207, 575
NspI RCATGY 1 cut(s) 113
PagI TCATGA 1 cut(s) 232
PauI GCGCGC 1 cut(s) 582
PciI ACATGT 1 cut(s) 109
PciSI GCTCTTC 1 cut(s) 145
PcsI WCGNNNNNNNCGW 1 cut(s) 482
PctI GAATGC 1 cut(s) 297
PfeI GAWTC 2 cut(s) 73, 818
PflMI CCANNNNNTGG 1 cut(s) 486
PleI GAGTC 3 cut(s) 214, 251, 692
PpsI GAGTC 3 cut(s) 214, 251, 692
PpuMI RGGWCCY 1 cut(s) 500
PscI ACATGT 1 cut(s) 109
Psp5II RGGWCCY 1 cut(s) 500
Psp6I CCWGG 1 cut(s) 27
PspEI GGTNACC 1 cut(s) 575
PspGI CCWGG 1 cut(s) 27
PspN4I GGNNCC 1 cut(s) 903
PspPI GGNCC 2 cut(s) 424, 500
PspPPI RGGWCCY 1 cut(s) 500
PsuI RGATCY 1 cut(s) 398
PteI GCGCGC 1 cut(s) 582
RsaI GTAC 3 cut(s) 645, 683, 861
RsaNI GTAC 3 cut(s) 644, 682, 860
SapI GCTCTTC 1 cut(s) 145
SaqAI TTAA 1 cut(s) 267
Sau3AI GATC 5 cut(s) 55, 196, 335, 398, 627
Sau96I GGNCC 2 cut(s) 424, 500
SchI GAGTC 3 cut(s) 215, 251, 692
ScrFI CCNGG 3 cut(s) 29, 544, 573
SduI GDGCHC 3 cut(s) 157, 380, 777
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 1 cut(s) 628
SinI GGWCC 1 cut(s) 500
Sse9I AATT 1 cut(s) 387
SsiI CCGC 3 cut(s) 422, 580, 856
SspI AATATT 1 cut(s) 283
SspMI CTAG 2 cut(s) 593, 656
StyD4I CCNGG 3 cut(s) 27, 542, 571
TaaI ACNGT 4 cut(s) 213, 302, 643, 843
TaqI TCGA 3 cut(s) 54, 117, 825
TasI AATT 1 cut(s) 387
TfiI GAWTC 2 cut(s) 73, 818
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 3 cut(s) 166, 692, 930
TseFI GTSAC 2 cut(s) 207, 575
Tsp45I GTSAC 2 cut(s) 207, 575
TspDTI ATGAA 3 cut(s) 205, 221, 249
TspGWI ACGGA 1 cut(s) 474
TspRI CASTG 3 cut(s) 166, 692, 930
Van91I CCANNNNNTGG 1 cut(s) 486
VpaK11BI GGWCC 1 cut(s) 500
XceI RCATGY 1 cut(s) 113
XspI CTAG 2 cut(s) 593, 656
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.