Rmu_sc0002141.1_g000005

Protein ROOT HAIR DEFECTIVE 3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002141.1
OG0019368
Physical Location & Seq
Forward (+)
14640 .. 26316
11677 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002141.1_g000005.1.cds

Sequence Viewer

Length: 1872 bp
atgaaagttgtagagctcgtcgatacgatcgcgtgcatatgcggaacgcaaaaatcggagttcctcggccggaacgactgcaatttcttctccggcgttgttcttgctccacaggccaccaaatcactccaaacttcttccattggcttcgcctcgtccttgcgcacccgtctgtggtggccttgcacggcggcgtggcctgtagcggcggtgctccggccaccaaaacttccgatccttagctccatgaactcgcctcaacctgctgatcatcatactagaaggattgcacttggagtgaccggtttcacgttcgtcagagctcggtcgaaagaatcgaggcctgaaattgaagtttttatgtcgaaaatcgagctcgccggtttcggcattttctcgccggtttctggaaattttccggcgaccttcatactgttcaaggtaatttttgaccccttccggtcattttcagacttcgtgctagttatgaaaattgttgggcttgatgagatgaaaaggagcagccaggccccgacaccattagcggtggtcggcggcggctctgtcctttttccggcgacccttttccggccacctccggggatatgggatgaagttccaaagcccctggcccgtaaatttagccgattcgatgataattttagggtggaagtggttggtttgcctcattacatccataagagggagaagtttaaggacgagaacgcgtcgatacgagttcgtggacatgcggaacgcgtaaatcggagttctatcaaagttcttcgactgacccgacccggacccggcattccgacccggtcggaactcgcagctccagcgaccacttccggtgaaaccaacccagaacgattcgcctccttgctccggtcgtctctgtggtggtctctatcgacgattctcattcacggcggcgctgcaagtcggcccagattgaggaaatcgaacccggccggaaaacacagctccgaggtcggcaaggcttcgatttttcttggttggactcagaacagcctcgccgatcgatccttggtggtggtttggatcgattcacgtgaaacttcggtcaactcagttgtgattactgttcacggcttagcacggcttctccggtctctctctccctccactggccaccaaacagcatcggaccatcttcattggcttcgtgtcgaccttgcgcagctgccagaggcagccttgaaccgccacacggccaccagcggcggcgggaagctccgccagctcgccggttctgcaaattctcgccggattctggaaattttccgaccaatccgactggttcgaggtaatttctatcccttccggtcattttcagctcacatgctagttaggaaagtggttgggcttgatgagatgaagaggagcagtctggccccgacaccattggaggtggtcagcggcggctctgccctaactccggcggcctttttccggccacctccgggagtcaaaaatcttaccgggtttggtgttgttgcaatcccggtacactattcaaattgtgtagcgggaaatcctacaggtcaggagaatcagagcgaaactacatcaaagtcctcaccacgaggcacaaagaatcctgcgacgaggttggtgctctcctcgtcctcaatcgctgaaaagaagtcaggtcaagggacacccccgacgactgcacccgaacgattgagcagagcgaaatcaccgggaagtgttgagatcttgctaaagcgtgactttagcttggctgggttgttagggcgttacccttgcttggctggttctgtaaccgttttggtcgcggcactaccgtcggctcccgaggagactaggaccgaagtacattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005783 GO:0012505 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RHD3

Protein Analysis

623

Amino Acids

67.63

Weight (kDa)

10.58

Isoelectric Point (pI)

54.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Style Settings
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 164, 1203
Acc36I ACCTGC 1 cut(s) 271
AccI GTMKAC 1 cut(s) 1194
AccII CGCG 4 cut(s) 32, 728, 759, 1826
AclWI GGATC 3 cut(s) 229, 1041, 1073
AcoI YGGCCR 7 cut(s) 67, 218, 590, 972, 1153, 1236, 1478
AcsI RAATTY 4 cut(s) 412, 638, 1282, 1302
AcvI CACGTG 1 cut(s) 1076
AfaI GTAC 2 cut(s) 1533, 1866
AflIII ACRYGT 2 cut(s) 726, 757
AgeI ACCGGT 1 cut(s) 302
AgsI TTSAA 4 cut(s) 353, 439, 1225, 1542
AjnI CCWGG 2 cut(s) 525, 627
Alw21I GWGCWC 5 cut(s) 18, 216, 325, 378, 1644
Alw26I GTCTC 4 cut(s) 900, 912, 1140, 1844
AlwI GGATC 3 cut(s) 229, 1041, 1073
Ama87I CYCGRG 1 cut(s) 1844
ApeKI GCWGC 6 cut(s) 522, 833, 938, 1204, 1207, 1217
ApoI RAATTY 4 cut(s) 412, 638, 1282, 1302
AsiGI ACCGGT 1 cut(s) 302
AspLEI GCGC 3 cut(s) 165, 938, 1204
AspS9I GGNCC 7 cut(s) 529, 631, 803, 948, 1171, 1417, 1857
AsuC2I CCSGG 9 cut(s) 600, 801, 807, 820, 971, 1488, 1507, 1529, 1731
AsuHPI GGTGA 3 cut(s) 866, 1596, 1719
AvaI CYCGRG 1 cut(s) 1844
AvaII GGWCC 3 cut(s) 803, 1171, 1857
BalI TGGCCA 1 cut(s) 1155
BanII GRGCYC 3 cut(s) 18, 325, 378
BauI CACGAG 1 cut(s) 1608
BbrPI CACGTG 1 cut(s) 1076
Bbv12I GWGCWC 5 cut(s) 18, 216, 325, 378, 1644
BbvI GCAGC 6 cut(s) 534, 845, 925, 1194, 1216, 1229
BccI CCATC 1 cut(s) 1182
BceAI ACGGC 5 cut(s) 204, 946, 1129, 1139, 1251
BcgI CGANNNNNNTGC 4 cut(s) 1621, 1655, 1818, 1852
BciT130I CCWGG 2 cut(s) 527, 629
BclI TGATCA 1 cut(s) 268
BcnI CCSGG 9 cut(s) 600, 801, 807, 820, 971, 1488, 1507, 1529, 1731
BcoDI GTCTC 4 cut(s) 900, 912, 1140, 1844
BfaI CTAG 4 cut(s) 279, 482, 1370, 1854
BfmI CTRYAG 2 cut(s) 201, 1563
BfoI RGCGCY 1 cut(s) 939
BfuAI ACCTGC 1 cut(s) 271
BglII AGATCT 1 cut(s) 1743
BlpI GCTNAGC 1 cut(s) 1117
Bme18I GGWCC 3 cut(s) 803, 1171, 1857
BmeT110I CYCGRG 1 cut(s) 1844
BmgT120I GGNCC 7 cut(s) 529, 631, 803, 948, 1171, 1417, 1857
BmiI GGNNCC 4 cut(s) 531, 805, 1419, 1842
BmsI GCATC 1 cut(s) 1175
BpmI CTGGAG 1 cut(s) 822
Bpu10I CCTNAGC 1 cut(s) 239
Bpu1102I GCTNAGC 1 cut(s) 1117
BpuMI CCSGG 9 cut(s) 600, 801, 807, 820, 971, 1488, 1507, 1529, 1731
Bsa29I ATCGAT 2 cut(s) 1045, 1068
BsaAI YACGTR 1 cut(s) 1076
BsaI GGTCTC 2 cut(s) 912, 1140
BsaJI CCNNGG 6 cut(s) 64, 599, 627, 990, 1050, 1845
BsaWI WCCGGW 6 cut(s) 302, 459, 851, 888, 1131, 1347
BsaXI ACNNNNNCTCC 2 cut(s) 1113, 1143
Bse118I RCCGGY 4 cut(s) 302, 380, 400, 1271
Bse1I ACTGG 2 cut(s) 1156, 1326
BseBI CCWGG 2 cut(s) 527, 629
BseCI ATCGAT 2 cut(s) 1045, 1068
BseDI CCNNGG 6 cut(s) 64, 599, 627, 990, 1050, 1845
BseGI GGATG 2 cut(s) 616, 693
BseMII CTCAG 2 cut(s) 1040, 1107
BseNI ACTGG 2 cut(s) 1156, 1326
BseRI GAGGAG 3 cut(s) 1420, 1636, 1862
BseX3I CGGCCG 2 cut(s) 67, 972
BseXI GCAGC 6 cut(s) 534, 845, 925, 1194, 1216, 1229
BseYI CCCAGC 1 cut(s) 1772
BsgI GTGCAG 1 cut(s) 1683
Bsh1236I CGCG 4 cut(s) 32, 728, 759, 1826
Bsh1285I CGRYCG 7 cut(s) 30, 70, 329, 824, 893, 975, 1045
BshTI ACCGGT 1 cut(s) 302
BshVI ATCGAT 2 cut(s) 1045, 1068
BsiEI CGRYCG 7 cut(s) 30, 70, 329, 824, 893, 975, 1045
BsiHKAI GWGCWC 5 cut(s) 18, 216, 325, 378, 1644
BsiHKCI CYCGRG 1 cut(s) 1844
BslFI GGGAC 1 cut(s) 1696
BsmAI GTCTC 4 cut(s) 900, 912, 1140, 1844
BsmBI CGTCTC 1 cut(s) 900
BsmFI GGGAC 1 cut(s) 1696
BsmI GAATGC 1 cut(s) 810
Bso31I GGTCTC 2 cut(s) 912, 1140
BsoBI CYCGRG 1 cut(s) 1844
Bsp1286I GDGCHC 5 cut(s) 18, 216, 325, 378, 1644
Bsp143I GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
Bsp1720I GCTNAGC 1 cut(s) 1117
BspCNI CTCAG 2 cut(s) 1039, 1106
BspDI ATCGAT 2 cut(s) 1045, 1068
BspFNI CGCG 4 cut(s) 32, 728, 759, 1826
BspLI GGNNCC 4 cut(s) 531, 805, 1419, 1842
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 3 cut(s) 229, 1041, 1073
BspTNI GGTCTC 2 cut(s) 912, 1140
BsrFI RCCGGY 4 cut(s) 302, 380, 400, 1271
BsrI ACTGG 2 cut(s) 1156, 1326
BssAI RCCGGY 4 cut(s) 302, 380, 400, 1271
BssECI CCNNGG 6 cut(s) 64, 599, 627, 990, 1050, 1845
BssMI GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
BssSI CACGAG 1 cut(s) 1608
BssT1I CCWWGG 1 cut(s) 1050
Bst2BI CACGAG 1 cut(s) 1608
Bst2UI CCWGG 2 cut(s) 527, 629
Bst4CI ACNGT 4 cut(s) 435, 1108, 1816, 1836
Bst6I CTCTTC 1 cut(s) 1397
BstBAI YACGTR 1 cut(s) 1076
BstC8I GCNNGC 4 cut(s) 34, 378, 1265, 1269
BstDEI CTNAG 4 cut(s) 239, 1026, 1093, 1117
BstF5I GGATG 2 cut(s) 616, 693
BstFNI CGCG 4 cut(s) 32, 728, 759, 1826
BstH2I RGCGCY 1 cut(s) 939
BstHHI GCGC 3 cut(s) 165, 938, 1204
BstKTI GATC 7 cut(s) 30, 237, 271, 1045, 1049, 1068, 1746
BstMAI GTCTC 4 cut(s) 900, 912, 1140, 1844
BstMBI GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
BstMCI CGRYCG 7 cut(s) 30, 70, 329, 824, 893, 975, 1045
BstMWI GCNNNNNNNGC 4 cut(s) 113, 839, 1264, 1277
BstNI CCWGG 2 cut(s) 527, 629
BstNSI RCATGY 2 cut(s) 752, 1369
BstSFI CTRYAG 2 cut(s) 201, 1563
BstUI CGCG 4 cut(s) 32, 728, 759, 1826
BstV1I GCAGC 6 cut(s) 534, 845, 925, 1194, 1216, 1229
BstX2I RGATCY 1 cut(s) 1743
BstYI RGATCY 1 cut(s) 1743
BstZI CGGCCG 2 cut(s) 67, 972
Bsu15I ATCGAT 2 cut(s) 1045, 1068
BsuTUI ATCGAT 2 cut(s) 1045, 1068
BtsCI GGATG 2 cut(s) 616, 693
BtsIMutI CAGTG 1 cut(s) 1149
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 4 cut(s) 34, 378, 1265, 1269
CfoI GCGC 3 cut(s) 165, 938, 1204
Cfr10I RCCGGY 4 cut(s) 302, 380, 400, 1271
Cfr13I GGNCC 7 cut(s) 529, 631, 803, 948, 1171, 1417, 1857
ClaI ATCGAT 2 cut(s) 1045, 1068
CseI GACGC 1 cut(s) 717
Csp6I GTAC 2 cut(s) 1532, 1865
CspAI ACCGGT 1 cut(s) 302
CviAII CATG 3 cut(s) 247, 749, 1366
CviQI GTAC 2 cut(s) 1532, 1865
DdeI CTNAG 4 cut(s) 239, 1026, 1093, 1117
DpnI GATC 7 cut(s) 29, 236, 270, 1044, 1048, 1067, 1745
DpnII GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
EaeI YGGCCR 7 cut(s) 67, 218, 590, 972, 1153, 1236, 1478
EagI CGGCCG 2 cut(s) 67, 972
Eam1104I CTCTTC 1 cut(s) 1397
EarI CTCTTC 1 cut(s) 1397
EciI GGCGGA 1 cut(s) 1250
Ecl136II GAGCTC 3 cut(s) 16, 323, 376
EclXI CGGCCG 2 cut(s) 67, 972
Eco130I CCWWGG 1 cut(s) 1050
Eco147I AGGCCT 1 cut(s) 343
Eco24I GRGCYC 3 cut(s) 18, 325, 378
Eco31I GGTCTC 2 cut(s) 912, 1140
Eco47I GGWCC 3 cut(s) 803, 1171, 1857
Eco52I CGGCCG 2 cut(s) 67, 972
Eco53kI GAGCTC 3 cut(s) 16, 323, 376
Eco72I CACGTG 1 cut(s) 1076
Eco88I CYCGRG 1 cut(s) 1844
EcoICRI GAGCTC 3 cut(s) 16, 323, 376
EcoO109I RGGNCCY 1 cut(s) 529
EcoRII CCWGG 2 cut(s) 525, 627
EcoT14I CCWWGG 1 cut(s) 1050
EcoT38I GRGCYC 3 cut(s) 18, 325, 378
ErhI CCWWGG 1 cut(s) 1050
Esp3I CGTCTC 1 cut(s) 900
FaeI CATG 3 cut(s) 250, 752, 1369
FalI AAGNNNNNCTT 2 cut(s) 1745, 1777
FaqI GGGAC 1 cut(s) 1696
FatI CATG 3 cut(s) 246, 748, 1365
FauI CCCGC 2 cut(s) 1244, 1546
FauNDI CATATG 1 cut(s) 38
FbaI TGATCA 1 cut(s) 268
FblI GTMKAC 1 cut(s) 1194
FokI GGATG 2 cut(s) 623, 680
FriOI GRGCYC 3 cut(s) 18, 325, 378
FspBI CTAG 4 cut(s) 279, 482, 1370, 1854
FspI TGCGCA 2 cut(s) 164, 1203
GlaI GCGC 3 cut(s) 164, 937, 1203
GsaI CCCAGC 1 cut(s) 1776
GsuI CTGGAG 1 cut(s) 822
HaeII RGCGCY 1 cut(s) 939
HgaI GACGC 1 cut(s) 717
HhaI GCGC 3 cut(s) 165, 938, 1204
Hin1II CATG 3 cut(s) 250, 752, 1369
Hin6I GCGC 3 cut(s) 163, 936, 1202
HinP1I GCGC 3 cut(s) 163, 936, 1202
HincII GTYRAC 2 cut(s) 1090, 1195
HindII GTYRAC 2 cut(s) 1090, 1195
HphI GGTGA 3 cut(s) 866, 1596, 1719
Hpy166II GTNNAC 5 cut(s) 746, 1090, 1111, 1195, 1534
Hpy188III TCNNGA 4 cut(s) 408, 1298, 1571, 1844
Hpy8I GTNNAC 5 cut(s) 746, 1090, 1111, 1195, 1534
Hpy99I CGWCG 6 cut(s) 23, 733, 919, 1633, 1696, 1840
HpyAV CCTTC 4 cut(s) 276, 436, 466, 1354
HpyCH4III ACNGT 4 cut(s) 435, 1108, 1816, 1836
HpyCH4IV ACGT 2 cut(s) 311, 1075
HpyCH4V TGCA 8 cut(s) 36, 81, 186, 290, 941, 1280, 1523, 1700
HpyF10VI GCNNNNNNNGC 4 cut(s) 113, 839, 1264, 1277
HpyF3I CTNAG 4 cut(s) 239, 1026, 1093, 1117
HpySE526I ACGT 2 cut(s) 311, 1075
Hsp92II CATG 3 cut(s) 250, 752, 1369
HspAI GCGC 3 cut(s) 163, 936, 1202
Ksp22I TGATCA 1 cut(s) 268
Kzo9I GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
Lsp1109I GCAGC 6 cut(s) 534, 845, 925, 1194, 1216, 1229
LweI GCATC 1 cut(s) 1175
MaeI CTAG 4 cut(s) 279, 482, 1370, 1854
MaeII ACGT 2 cut(s) 311, 1075
MaeIII GTNAC 4 cut(s) 298, 1757, 1787, 1810
MalI GATC 7 cut(s) 29, 236, 270, 1044, 1048, 1067, 1745
MboI GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
MboII GAAGA 5 cut(s) 79, 129, 776, 1169, 1414
MflI RGATCY 1 cut(s) 1743
MhlI GDGCHC 5 cut(s) 18, 216, 325, 378, 1644
MlsI TGGCCA 1 cut(s) 1155
MluI ACGCGT 2 cut(s) 726, 757
MluNI TGGCCA 1 cut(s) 1155
MlyI GAGTC 2 cut(s) 1018, 1500
MmeI TCCRAC 5 cut(s) 804, 839, 1001, 1333, 1342
Mox20I TGGCCA 1 cut(s) 1155
MscI TGGCCA 1 cut(s) 1155
MseI TTAA 1 cut(s) 714
Msp20I TGGCCA 1 cut(s) 1155
MspA1I CMGCKG 3 cut(s) 1207, 1245, 1443
Mva1269I GAATGC 1 cut(s) 810
MvaI CCWGG 2 cut(s) 527, 629
MvnI CGCG 4 cut(s) 32, 728, 759, 1826
MwoI GCNNNNNNNGC 4 cut(s) 113, 839, 1264, 1277
NciI CCSGG 9 cut(s) 600, 801, 807, 820, 971, 1488, 1507, 1529, 1731
NdeI CATATG 1 cut(s) 38
NdeII GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
NlaIII CATG 3 cut(s) 250, 752, 1369
NlaIV GGNNCC 4 cut(s) 531, 805, 1419, 1842
NmeAIII GCCGAG 1 cut(s) 45
NmuCI GTSAC 2 cut(s) 298, 1757
NsbI TGCGCA 2 cut(s) 164, 1203
NspI RCATGY 2 cut(s) 752, 1369
PceI AGGCCT 1 cut(s) 343
PcsI WCGNNNNNNNCGW 3 cut(s) 72, 335, 793
PctI GAATGC 1 cut(s) 810
PfeI GAWTC 8 cut(s) 335, 648, 873, 919, 1070, 1294, 1576, 1621
PflFI GACNNNGTC 1 cut(s) 820
PfoI TCCNGGA 1 cut(s) 1486
PinAI ACCGGT 1 cut(s) 302
Ple19I CGATCG 2 cut(s) 30, 1045
PleI GAGTC 2 cut(s) 1018, 1499
PmaCI CACGTG 1 cut(s) 1076
PmlI CACGTG 1 cut(s) 1076
PpsI GAGTC 2 cut(s) 1018, 1499
Ppu21I YACGTR 1 cut(s) 1076
Psp124BI GAGCTC 3 cut(s) 18, 325, 378
Psp6I CCWGG 2 cut(s) 525, 627
PspCI CACGTG 1 cut(s) 1076
PspFI CCCAGC 1 cut(s) 1772
PspGI CCWGG 2 cut(s) 525, 627
PspN4I GGNNCC 4 cut(s) 531, 805, 1419, 1842
PspPI GGNCC 7 cut(s) 529, 631, 803, 948, 1171, 1417, 1857
PsuI RGATCY 1 cut(s) 1743
PsyI GACNNNGTC 1 cut(s) 820
PvuI CGATCG 2 cut(s) 30, 1045
PvuII CAGCTG 1 cut(s) 1207
RsaI GTAC 2 cut(s) 1533, 1866
RsaNI GTAC 2 cut(s) 1532, 1865
SacI GAGCTC 3 cut(s) 18, 325, 378
SalI GTCGAC 1 cut(s) 1193
SaqAI TTAA 1 cut(s) 714
Sau3AI GATC 7 cut(s) 27, 234, 268, 1042, 1046, 1065, 1743
Sau96I GGNCC 7 cut(s) 529, 631, 803, 948, 1171, 1417, 1857
SchI GAGTC 2 cut(s) 1018, 1500
SduI GDGCHC 5 cut(s) 18, 216, 325, 378, 1644
SfaNI GCATC 1 cut(s) 1175
SfcI CTRYAG 2 cut(s) 201, 1563
SinI GGWCC 3 cut(s) 803, 1171, 1857
SseBI AGGCCT 1 cut(s) 343
SspMI CTAG 4 cut(s) 279, 482, 1370, 1854
SstI GAGCTC 3 cut(s) 18, 325, 378
StuI AGGCCT 1 cut(s) 343
StyI CCWWGG 1 cut(s) 1050
TaaI ACNGT 4 cut(s) 435, 1108, 1816, 1836
TaiI ACGT 2 cut(s) 314, 1078
TaqII GACCGA 2 cut(s) 315, 1075
TatI WGTACW 1 cut(s) 1864
TfiI GAWTC 8 cut(s) 335, 648, 873, 919, 1070, 1294, 1576, 1621
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TscAI CASTG 1 cut(s) 1156
TseFI GTSAC 2 cut(s) 298, 1757
TseI GCWGC 6 cut(s) 522, 833, 938, 1204, 1207, 1217
Tsp45I GTSAC 2 cut(s) 298, 1757
TspDTI ATGAA 8 cut(s) 17, 263, 418, 503, 527, 627, 1169, 1415
TspRI CASTG 1 cut(s) 1156
Tth111I GACNNNGTC 1 cut(s) 820
VpaK11BI GGWCC 3 cut(s) 803, 1171, 1857
XapI RAATTY 4 cut(s) 412, 638, 1282, 1302
XceI RCATGY 2 cut(s) 752, 1369
XmiI GTMKAC 1 cut(s) 1194
XspI CTAG 4 cut(s) 279, 482, 1370, 1854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.