Rmu_sc0002251.1_g000016

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002251.1
OG0014698
Physical Location & Seq
Forward (+)
61137 .. 62252
1116 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1116 bp
atggattgcatgtctgttagtgaggtttgcaaatcttggagaaatgttgttgcacaagaactttctggttggcaagggcatgggtttccatggctcttaatgtctggtgagaaagacaaaagggtaaggacttgtgttagcatactacaggagcaagagtgggagatgttgcttccggaggcttatggtagatacttctgggggtcataccatgactggttgattctagtaaaagatcttggctctttcaggcttgatattagcttgtttaacccattcactcggaaacaaattgatcttcccggagcatggaacttctatcacaagatggttctcccaaggcttccctgtcttgaaaactttatctgcatgctggttcatagtcactcccagaagcttgccttttgggtcccaggagctcaatcatggcttgagcataagctagatggtgaaccatttgtggatgctgtcttctccaatggaagtttctatctcattggcaatgattataacatttgggaaattaagggtgctgatatttttgctgccattaacagagtcaatgcatctgcccctgatgcttttgatgtcgaaaggcatttccatgaagtaactatgtcagatgagcatgaaaataatggtgtgctaaagtatcttgttgagtcttgtggggagcttctacttgtttgtaggttctatagtactaacccaggtgctgtgctggtaacaaattattttaaggtatattcactggattttagccaaatgtcctggaagagggtagagagtttgggagatcgaatactatttctgggcaagtgttgttcaagatctgtctcttccagtggacttggtcttaatgcgactgattgtatctatttctctaatgatcatgctgttccgtggtggaacgaatgggattctgatcatcttgatggcatttcaactcgtcttgggctggacaatagtgggagaaaggattggggctttttcagcctcaacaacaaaaatcatgaaactttgtctattcggttccgtggcaatcgagatagatggggacctgtttggtttactgcgccgcagtggtggtgttgtaagaatttggttcttgcctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

371

Amino Acids

42.89

Weight (kDa)

5.84

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 56 - 307 6.8e-46 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014698)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27200
malus_domestica MD13G1035900.v1.1
prunus_persica Prupe.6G174300_v2.0.a1 Prupe.6G178100_v2.0.a1
pyrus_communis pycom13g03130 pycom16g03310
rosa_chinensis RchiOBHm_Chr4g0434481
rosa_laevigata RLG00000006668
rosa_multiflora Rmu_sc0002251.1_g000016 Rmu_sc0005899.1_g000009
rosa_roxburghii Rroxscaffold_5G00375830
rosa_rugosa Rorug04G0277000
rosa_samantha Rh4AG332600 Rh4BG340400 Rh4CG355600 Rh4DG335800
rosa_wichuraiana Rw4G028840

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 510
AccIII TCCGGA 1 cut(s) 175
AciI CCGC 1 cut(s) 1079
AcsI RAATTY 1 cut(s) 1099
AfaI GTAC 1 cut(s) 703
AfiI CCNNNNNNNGG 2 cut(s) 309, 777
AgsI TTSAA 3 cut(s) 356, 828, 945
AjnI CCWGG 3 cut(s) 412, 709, 770
AjuI GAANNNNNNNTTGG 2 cut(s) 470, 502
AluBI AGCT 5 cut(s) 264, 397, 419, 442, 676
AluI AGCT 5 cut(s) 264, 397, 419, 442, 676
Alw21I GWGCWC 1 cut(s) 421
Alw26I GTCTC 1 cut(s) 841
AlwNI CAGNNNCTG 1 cut(s) 716
Aor13HI TCCGGA 1 cut(s) 175
ApeKI GCWGC 1 cut(s) 545
ApoI RAATTY 1 cut(s) 1099
AspLEI GCGC 1 cut(s) 1078
AspS9I GGNCC 2 cut(s) 409, 1058
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 2 cut(s) 119, 461
AvaII GGWCC 2 cut(s) 409, 1058
BanII GRGCYC 1 cut(s) 421
BbsI GAAGAC 1 cut(s) 463
Bbv12I GWGCWC 1 cut(s) 421
BbvI GCAGC 1 cut(s) 532
BccI CCATC 4 cut(s) 322, 440, 929, 1047
BciT130I CCWGG 3 cut(s) 414, 711, 772
BclI TGATCA 2 cut(s) 889, 925
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 1 cut(s) 841
BfaI CTAG 2 cut(s) 227, 443
BfmI CTRYAG 2 cut(s) 146, 697
BglII AGATCT 2 cut(s) 235, 830
BisI GCNGC 2 cut(s) 546, 1079
BlsI GCNGC 2 cut(s) 547, 1080
BmcAI AGTACT 1 cut(s) 703
Bme1390I CCNGG 4 cut(s) 303, 414, 711, 772
Bme18I GGWCC 2 cut(s) 409, 1058
BmgT120I GGNCC 2 cut(s) 409, 1058
BmiI GGNNCC 4 cut(s) 410, 411, 1034, 1059
BmrFI CCNGG 4 cut(s) 303, 414, 711, 772
BmsI GCATC 3 cut(s) 454, 568, 575
BpiI GAAGAC 1 cut(s) 463
BpuEI CTTGAG 1 cut(s) 452
BpuMI CCSGG 1 cut(s) 303
BsaBI GATNNNNATC 1 cut(s) 924
BsaJI CCNNGG 6 cut(s) 89, 338, 412, 709, 902, 1036
BsaWI WCCGGW 1 cut(s) 175
BsaXI ACNNNNNCTCC 2 cut(s) 143, 173
Bsc4I CCNNNNNNNGG 2 cut(s) 309, 777
Bse1I ACTGG 3 cut(s) 221, 756, 843
Bse3DI GCAATG 1 cut(s) 508
Bse8I GATNNNNATC 1 cut(s) 924
BseAI TCCGGA 1 cut(s) 175
BseBI CCWGG 3 cut(s) 414, 711, 772
BseDI CCNNGG 6 cut(s) 89, 338, 412, 709, 902, 1036
BseGI GGATG 1 cut(s) 469
BseJI GATNNNNATC 1 cut(s) 924
BseLI CCNNNNNNNGG 2 cut(s) 309, 777
BseMI GCAATG 1 cut(s) 508
BseNI ACTGG 3 cut(s) 221, 756, 843
BseXI GCAGC 1 cut(s) 532
BsiHKAI GWGCWC 1 cut(s) 421
BsiSI CCGG 2 cut(s) 176, 303
BslFI GGGAC 2 cut(s) 395, 1071
BslI CCNNNNNNNGG 2 cut(s) 309, 777
BsmAI GTCTC 1 cut(s) 841
BsmFI GGGAC 2 cut(s) 395, 1071
Bsp1286I GDGCHC 1 cut(s) 421
Bsp13I TCCGGA 1 cut(s) 175
Bsp143I GATC 6 cut(s) 235, 295, 796, 830, 889, 925
Bsp19I CCATGG 1 cut(s) 89
BspACI CCGC 1 cut(s) 1079
BspEI TCCGGA 1 cut(s) 175
BspHI TCATGA 1 cut(s) 1012
BspLI GGNNCC 4 cut(s) 410, 411, 1034, 1059
BsrDI GCAATG 1 cut(s) 508
BsrI ACTGG 3 cut(s) 221, 756, 843
BssECI CCNNGG 6 cut(s) 89, 338, 412, 709, 902, 1036
BssMI GATC 6 cut(s) 235, 295, 796, 830, 889, 925
BssT1I CCWWGG 2 cut(s) 89, 338
Bst2UI CCWGG 3 cut(s) 414, 711, 772
Bst6I CTCTTC 2 cut(s) 770, 844
BstC8I GCNNGC 2 cut(s) 371, 399
BstDSI CCRYGG 3 cut(s) 89, 902, 1036
BstENI CCTNNNNNAGG 1 cut(s) 775
BstF5I GGATG 1 cut(s) 469
BstHHI GCGC 1 cut(s) 1078
BstKTI GATC 6 cut(s) 238, 298, 799, 833, 892, 928
BstMAI GTCTC 1 cut(s) 841
BstMBI GATC 6 cut(s) 235, 295, 796, 830, 889, 925
BstMWI GCNNNNNNNGC 2 cut(s) 578, 993
BstNI CCWGG 3 cut(s) 414, 711, 772
BstNSI RCATGY 2 cut(s) 13, 373
BstSCI CCNGG 4 cut(s) 301, 412, 709, 770
BstSFI CTRYAG 2 cut(s) 146, 697
BstV1I GCAGC 1 cut(s) 532
BstV2I GAAGAC 1 cut(s) 463
BstX2I RGATCY 2 cut(s) 235, 830
BstYI RGATCY 2 cut(s) 235, 830
BtgI CCRYGG 3 cut(s) 89, 902, 1036
BtsCI GGATG 1 cut(s) 469
BtsI GCAGTG 1 cut(s) 1088
BtsIMutI CAGTG 3 cut(s) 749, 850, 1088
Cac8I GCNNGC 2 cut(s) 371, 399
CaiI CAGNNNCTG 1 cut(s) 716
CciI TCATGA 1 cut(s) 1012
CfoI GCGC 1 cut(s) 1078
Cfr13I GGNCC 2 cut(s) 409, 1058
Csp6I GTAC 1 cut(s) 702
CviQI GTAC 1 cut(s) 702
DpnI GATC 6 cut(s) 237, 297, 798, 832, 891, 927
DpnII GATC 6 cut(s) 235, 295, 796, 830, 889, 925
Eam1104I CTCTTC 2 cut(s) 770, 844
EarI CTCTTC 2 cut(s) 770, 844
Ecl136II GAGCTC 1 cut(s) 419
Eco130I CCWWGG 2 cut(s) 89, 338
Eco24I GRGCYC 1 cut(s) 421
Eco47I GGWCC 2 cut(s) 409, 1058
Eco53kI GAGCTC 1 cut(s) 419
EcoICRI GAGCTC 1 cut(s) 419
EcoNI CCTNNNNNAGG 1 cut(s) 775
EcoO109I RGGNCCY 2 cut(s) 409, 1058
EcoRII CCWGG 3 cut(s) 412, 709, 770
EcoT14I CCWWGG 2 cut(s) 89, 338
EcoT22I ATGCAT 1 cut(s) 568
EcoT38I GRGCYC 1 cut(s) 421
ErhI CCWWGG 2 cut(s) 89, 338
FalI AAGNNNNNCTT 2 cut(s) 386, 418
FaqI GGGAC 2 cut(s) 395, 1071
FbaI TGATCA 2 cut(s) 889, 925
Fnu4HI GCNGC 2 cut(s) 546, 1079
FokI GGATG 1 cut(s) 476
FriOI GRGCYC 1 cut(s) 421
Fsp4HI GCNGC 2 cut(s) 546, 1079
FspBI CTAG 2 cut(s) 227, 443
GlaI GCGC 1 cut(s) 1077
GluI GCNGC 2 cut(s) 546, 1079
HapII CCGG 2 cut(s) 176, 303
HhaI GCGC 1 cut(s) 1078
Hin6I GCGC 1 cut(s) 1076
HinP1I GCGC 1 cut(s) 1076
HindIII AAGCTT 1 cut(s) 395
HinfI GANTC 4 cut(s) 223, 558, 662, 920
HpaII CCGG 2 cut(s) 176, 303
HphI GGTGA 2 cut(s) 119, 461
Hpy166II GTNNAC 3 cut(s) 452, 848, 1071
Hpy188I TCNGA 3 cut(s) 285, 622, 925
Hpy188III TCNNGA 6 cut(s) 176, 353, 828, 932, 1013, 1046
Hpy8I GTNNAC 3 cut(s) 452, 848, 1071
HpyCH4V TGCA 5 cut(s) 9, 30, 53, 369, 566
HpyF10VI GCNNNNNNNGC 2 cut(s) 578, 993
HspAI GCGC 1 cut(s) 1076
KflI GGGWCCC 1 cut(s) 409
Kpn2I TCCGGA 1 cut(s) 175
Ksp22I TGATCA 2 cut(s) 889, 925
Kzo9I GATC 6 cut(s) 235, 295, 796, 830, 889, 925
LmnI GCTCC 4 cut(s) 151, 305, 416, 673
Lsp1109I GCAGC 1 cut(s) 532
LweI GCATC 3 cut(s) 454, 568, 575
MaeI CTAG 2 cut(s) 227, 443
MaeIII GTNAC 3 cut(s) 383, 610, 724
MalI GATC 6 cut(s) 237, 297, 798, 832, 891, 927
MboI GATC 6 cut(s) 235, 295, 796, 830, 889, 925
MboII GAAGA 4 cut(s) 290, 463, 787, 831
MflI RGATCY 2 cut(s) 235, 830
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 4 cut(s) 291, 522, 730, 1099
MlyI GAGTC 2 cut(s) 567, 671
MnlI CCTC 4 cut(s) 16, 172, 771, 1007
Mph1103I ATGCAT 1 cut(s) 568
MroI TCCGGA 1 cut(s) 175
MseI TTAA 6 cut(s) 98, 270, 525, 552, 738, 858
MslI CAYNNNNRTG 2 cut(s) 603, 933
MspI CCGG 2 cut(s) 176, 303
MspR9I CCNGG 4 cut(s) 303, 414, 711, 772
MvaI CCWGG 3 cut(s) 414, 711, 772
MwoI GCNNNNNNNGC 2 cut(s) 578, 993
NciI CCSGG 1 cut(s) 303
NcoI CCATGG 1 cut(s) 89
NdeII GATC 6 cut(s) 235, 295, 796, 830, 889, 925
NlaIV GGNNCC 4 cut(s) 410, 411, 1034, 1059
NmuCI GTSAC 1 cut(s) 383
NsiI ATGCAT 1 cut(s) 568
NspI RCATGY 2 cut(s) 13, 373
PaeI GCATGC 1 cut(s) 373
PagI TCATGA 1 cut(s) 1012
PfeI GAWTC 2 cut(s) 223, 920
PflFI GACNNNGTC 1 cut(s) 852
PfoI TCCNGGA 2 cut(s) 301, 770
PkrI GCNGC 2 cut(s) 547, 1080
PleI GAGTC 2 cut(s) 566, 670
PpsI GAGTC 2 cut(s) 566, 670
PpuMI RGGWCCY 2 cut(s) 409, 1058
PsiI TTATAA 1 cut(s) 510
Psp124BI GAGCTC 1 cut(s) 421
Psp5II RGGWCCY 2 cut(s) 409, 1058
Psp6I CCWGG 3 cut(s) 412, 709, 770
PspGI CCWGG 3 cut(s) 412, 709, 770
PspN4I GGNNCC 4 cut(s) 410, 411, 1034, 1059
PspPI GGNCC 2 cut(s) 409, 1058
PspPPI RGGWCCY 2 cut(s) 409, 1058
PstNI CAGNNNCTG 1 cut(s) 716
PsuI RGATCY 2 cut(s) 235, 830
PsyI GACNNNGTC 1 cut(s) 852
RsaI GTAC 1 cut(s) 703
RsaNI GTAC 1 cut(s) 702
RseI CAYNNNNRTG 2 cut(s) 603, 933
SacI GAGCTC 1 cut(s) 421
SaqAI TTAA 6 cut(s) 98, 270, 525, 552, 738, 858
SatI GCNGC 2 cut(s) 546, 1079
Sau3AI GATC 6 cut(s) 235, 295, 796, 830, 889, 925
Sau96I GGNCC 2 cut(s) 409, 1058
ScaI AGTACT 1 cut(s) 703
SchI GAGTC 2 cut(s) 567, 671
ScrFI CCNGG 4 cut(s) 303, 414, 711, 772
SduI GDGCHC 1 cut(s) 421
SfaNI GCATC 3 cut(s) 454, 568, 575
SfcI CTRYAG 2 cut(s) 146, 697
SinI GGWCC 2 cut(s) 409, 1058
SmiMI CAYNNNNRTG 2 cut(s) 603, 933
SmlI CTYRAG 1 cut(s) 431
SmoI CTYRAG 1 cut(s) 431
SphI GCATGC 1 cut(s) 373
Sse9I AATT 4 cut(s) 291, 522, 730, 1099
SsiI CCGC 1 cut(s) 1079
SspMI CTAG 2 cut(s) 227, 443
SstI GAGCTC 1 cut(s) 421
StyD4I CCNGG 4 cut(s) 301, 412, 709, 770
StyI CCWWGG 2 cut(s) 89, 338
TaqI TCGA 3 cut(s) 591, 799, 1045
TasI AATT 4 cut(s) 291, 522, 730, 1099
TatI WGTACW 1 cut(s) 701
TauI GCSGC 1 cut(s) 1081
TfiI GAWTC 2 cut(s) 223, 920
Tru1I TTAA 6 cut(s) 98, 270, 525, 552, 738, 858
Tru9I TTAA 6 cut(s) 98, 270, 525, 552, 738, 858
TscAI CASTG 3 cut(s) 756, 850, 1088
TseFI GTSAC 1 cut(s) 383
TseI GCWGC 1 cut(s) 545
Tsp45I GTSAC 1 cut(s) 383
TspDTI ATGAA 4 cut(s) 368, 621, 645, 1029
TspGWI ACGGA 2 cut(s) 891, 1025
TspRI CASTG 3 cut(s) 756, 850, 1088
Tth111I GACNNNGTC 1 cut(s) 852
VpaK11BI GGWCC 2 cut(s) 409, 1058
XagI CCTNNNNNAGG 1 cut(s) 775
XapI RAATTY 1 cut(s) 1099
XceI RCATGY 2 cut(s) 13, 373
XspI CTAG 2 cut(s) 227, 443
ZrmI AGTACT 1 cut(s) 703
Zsp2I ATGCAT 1 cut(s) 568
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.