Rmu_sc0002414.1_g000040

Replication protein A 14 kDa subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002414.1
OG0013004
Physical Location & Seq
Forward (+)
142073 .. 146584
4512 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 651 bp
atggtcagacttgcaaatagaaggaactcagctgttaactggagaagcaaggtgggacgaagggtagagaaattattgaagaaaaatgcagcttgggaaagtgattacagggcattacagtccaatgattggaggtttgaagtacaagaagatgagatctgtgtggactgccatgtggctggtctccagatgattttgtctctaaatgagttgagaaaaccctcagattcttcttcatcttttgccgcgctctccgtcgctcaatcttccaaactcaagttctattctcgactcagtttctccggcgatctcctccgatcatcaacagatatggatacgtcaaacccgtccgtttttgtgaatgcagagctgctgcggttgtatgttggaaggcaggttcgggcgttgattcaggttgtgcaactggatgctggatctgtcattggcaaatctactgatgaaattcagttaattgtaaagggcaccccaccctttcctcttacaaaatttgttgaggtcataggtattgctgacggcgataaatctatccaagctagcatatggaacaactttggtgacacaattgacacacatgcttacaatcagctttgcttactcgcaaatggagaatttaagcacttgttcatctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03030 ko03420 ko03430 ko03440 ko03460 map03030 map03420 map03430 map03440 map03460
Pfam Domains
Protein Families
Rep_fac-A_3

Protein Analysis

216

Amino Acids

24.19

Weight (kDa)

8.42

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rep_fac-A_3 PF08661 111 - 212 1.2e-19 Replication factor A protein 3
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013004)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52630 AT3G52630 AT4G18590
fragaria_vesca FvH4_6g18910
malus_domestica MD12G1025300.v1.1 MD14G1024000.v1.1
prunus_persica Prupe.7G108800_v2.0.a1
pyrus_communis pycom12g02480
rosa_chinensis RchiOBHm_Chr3g0473901
rosa_laevigata RLG00000023972
rosa_multiflora Rmu_sc0002414.1_g000040
rosa_roxburghii Rroxscaffold_6G00407670
rosa_rugosa Rorug03G0136800
rosa_samantha Rh3AG187000 Rh3BG215600 Rh3CG212300 Rh3DG211500
rosa_wichuraiana Rw3G017120

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 385
AccB1I GGYRCC 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 129
AccII CGCG 1 cut(s) 248
AciI CCGC 2 cut(s) 246, 376
AclWI GGATC 1 cut(s) 442
AcsI RAATTY 3 cut(s) 462, 506, 629
AfaI GTAC 1 cut(s) 144
AfiI CCNNNNNNNGG 1 cut(s) 129
AgsI TTSAA 2 cut(s) 79, 140
AjuI GAANNNNNNNTTGG 2 cut(s) 263, 295
AloI GAACNNNNNNTCC 2 cut(s) 381, 413
AluBI AGCT 5 cut(s) 32, 92, 370, 554, 607
AluI AGCT 5 cut(s) 32, 92, 370, 554, 607
Alw26I GTCTC 2 cut(s) 188, 204
AlwI GGATC 1 cut(s) 442
ApeKI GCWGC 3 cut(s) 89, 370, 373
ApoI RAATTY 3 cut(s) 462, 506, 629
AspLEI GCGC 1 cut(s) 250
AsuHPI GGTGA 1 cut(s) 587
AsuNHI GCTAGC 1 cut(s) 554
BaeGI GKGCMC 1 cut(s) 485
BanI GGYRCC 1 cut(s) 482
BbvI GCAGC 3 cut(s) 101, 357, 360
BceAI ACGGC 1 cut(s) 550
BciVI GTATCC 1 cut(s) 328
BcoDI GTCTC 2 cut(s) 188, 204
BfaI CTAG 1 cut(s) 555
BfuAI ACCTGC 1 cut(s) 385
BfuI GTATCC 1 cut(s) 328
BglII AGATCT 1 cut(s) 156
BisI GCNGC 4 cut(s) 90, 246, 371, 374
BlsI GCNGC 4 cut(s) 91, 247, 372, 375
BmiI GGNNCC 1 cut(s) 484
BmsI GCATC 1 cut(s) 418
BmtI GCTAGC 1 cut(s) 558
BpmI CTGGAG 2 cut(s) 61, 170
BpuEI CTTGAG 1 cut(s) 260
BsaI GGTCTC 1 cut(s) 188
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse1I ACTGG 2 cut(s) 44, 429
BseGI GGATG 1 cut(s) 433
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMII CTCAG 3 cut(s) 42, 237, 307
BseNI ACTGG 2 cut(s) 44, 429
BseRI GAGGAG 1 cut(s) 302
BseSI GKGCMC 1 cut(s) 485
BseXI GCAGC 3 cut(s) 101, 357, 360
Bsh1236I CGCG 1 cut(s) 248
BshNI GGYRCC 1 cut(s) 482
BsiSI CCGG 1 cut(s) 303
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 1 cut(s) 129
BsmAI GTCTC 2 cut(s) 188, 204
BsmFI GGGAC 1 cut(s) 69
BsmI GAATGC 1 cut(s) 367
Bso31I GGTCTC 1 cut(s) 188
Bsp1286I GDGCHC 1 cut(s) 485
Bsp143I GATC 4 cut(s) 156, 307, 317, 434
BspACI CCGC 2 cut(s) 246, 376
BspCNI CTCAG 3 cut(s) 41, 236, 306
BspFNI CGCG 1 cut(s) 248
BspLI GGNNCC 1 cut(s) 484
BspMI ACCTGC 1 cut(s) 385
BspOI GCTAGC 1 cut(s) 558
BspPI GGATC 1 cut(s) 442
BspT107I GGYRCC 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 188
BsrI ACTGG 2 cut(s) 44, 429
BssMI GATC 4 cut(s) 156, 307, 317, 434
Bst4CI ACNGT 1 cut(s) 120
BstC8I GCNNGC 1 cut(s) 556
BstDEI CTNAG 3 cut(s) 28, 223, 293
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 1 cut(s) 248
BstHHI GCGC 1 cut(s) 250
BstKTI GATC 4 cut(s) 159, 310, 320, 437
BstMAI GTCTC 2 cut(s) 188, 204
BstMBI GATC 4 cut(s) 156, 307, 317, 434
BstNSI RCATGY 1 cut(s) 596
BstSLI GKGCMC 1 cut(s) 485
BstUI CGCG 1 cut(s) 248
BstV1I GCAGC 3 cut(s) 101, 357, 360
BstX2I RGATCY 2 cut(s) 156, 434
BstXI CCANNNNNNTGG 1 cut(s) 179
BstYI RGATCY 2 cut(s) 156, 434
BsuI GTATCC 1 cut(s) 328
BtsCI GGATG 1 cut(s) 433
BveI ACCTGC 1 cut(s) 385
Cac8I GCNNGC 1 cut(s) 556
CfoI GCGC 1 cut(s) 250
Csp6I GTAC 1 cut(s) 143
CviAII CATG 2 cut(s) 173, 593
CviJI RGCY 6 cut(s) 32, 92, 179, 370, 554, 607
CviKI_1 RGCY 6 cut(s) 32, 92, 179, 370, 554, 607
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 3 cut(s) 28, 223, 293
DpnI GATC 4 cut(s) 158, 309, 319, 436
DpnII GATC 4 cut(s) 156, 307, 317, 434
Eco31I GGTCTC 1 cut(s) 188
FaeI CATG 2 cut(s) 176, 596
FaiI YATR 7 cut(s) 174, 332, 384, 521, 560, 562, 594
FaqI GGGAC 1 cut(s) 69
FatI CATG 2 cut(s) 172, 592
FauNDI CATATG 1 cut(s) 560
Fnu4HI GCNGC 4 cut(s) 90, 246, 371, 374
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 90, 246, 371, 374
FspBI CTAG 1 cut(s) 555
GlaI GCGC 1 cut(s) 249
GluI GCNGC 4 cut(s) 90, 246, 371, 374
GsuI CTGGAG 2 cut(s) 61, 170
HapII CCGG 1 cut(s) 303
HhaI GCGC 1 cut(s) 250
Hin1II CATG 2 cut(s) 176, 596
Hin6I GCGC 1 cut(s) 248
HinP1I GCGC 1 cut(s) 248
HincII GTYRAC 1 cut(s) 37
HindII GTYRAC 1 cut(s) 37
HinfI GANTC 3 cut(s) 227, 291, 409
HpaI GTTAAC 1 cut(s) 37
HpaII CCGG 1 cut(s) 303
HphI GGTGA 1 cut(s) 587
Hpy166II GTNNAC 2 cut(s) 37, 166
Hpy188I TCNGA 4 cut(s) 8, 226, 317, 650
Hpy188III TCNNGA 2 cut(s) 187, 288
Hpy8I GTNNAC 2 cut(s) 37, 166
Hpy99I CGWCG 1 cut(s) 260
HpyAV CCTTC 3 cut(s) 15, 54, 384
HpyCH4III ACNGT 1 cut(s) 120
HpyCH4IV ACGT 1 cut(s) 338
HpyCH4V TGCA 4 cut(s) 14, 89, 365, 421
HpyF3I CTNAG 3 cut(s) 28, 223, 293
HpySE526I ACGT 1 cut(s) 338
Hsp92II CATG 2 cut(s) 176, 596
HspAI GCGC 1 cut(s) 248
KspAI GTTAAC 1 cut(s) 37
Kzo9I GATC 4 cut(s) 156, 307, 317, 434
LpnPI CCDG 9 cut(s) 25, 94, 165, 200, 316, 380, 398, 410, 417
Lsp1109I GCAGC 3 cut(s) 101, 357, 360
LweI GCATC 1 cut(s) 418
MaeI CTAG 1 cut(s) 555
MaeII ACGT 1 cut(s) 338
MaeIII GTNAC 1 cut(s) 575
MalI GATC 4 cut(s) 158, 309, 319, 436
MboI GATC 4 cut(s) 156, 307, 317, 434
MboII GAAGA 5 cut(s) 91, 161, 222, 225, 258
MfeI CAATTG 1 cut(s) 582
MflI RGATCY 2 cut(s) 156, 434
MhlI GDGCHC 1 cut(s) 485
MluCI AATT 6 cut(s) 71, 462, 471, 506, 582, 629
MlyI GAGTC 1 cut(s) 285
MmeI TCCRAC 1 cut(s) 367
MnlI CCTC 5 cut(s) 126, 232, 323, 507, 508
MseI TTAA 3 cut(s) 36, 470, 633
MspA1I CMGCKG 1 cut(s) 32
MspI CCGG 1 cut(s) 303
MunI CAATTG 1 cut(s) 582
Mva1269I GAATGC 1 cut(s) 367
MvnI CGCG 1 cut(s) 248
NdeI CATATG 1 cut(s) 560
NdeII GATC 4 cut(s) 156, 307, 317, 434
NheI GCTAGC 1 cut(s) 554
NlaIII CATG 2 cut(s) 176, 596
NlaIV GGNNCC 1 cut(s) 484
NmuCI GTSAC 1 cut(s) 575
NspI RCATGY 1 cut(s) 596
PcsI WCGNNNNNNNCGW 1 cut(s) 344
PctI GAATGC 1 cut(s) 367
PfeI GAWTC 2 cut(s) 227, 409
PflMI CCANNNNNTGG 1 cut(s) 129
PkrI GCNGC 4 cut(s) 91, 247, 372, 375
PleI GAGTC 1 cut(s) 285
PpsI GAGTC 1 cut(s) 285
PspN4I GGNNCC 1 cut(s) 484
PsuI RGATCY 2 cut(s) 156, 434
PvuII CAGCTG 1 cut(s) 32
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SaqAI TTAA 3 cut(s) 36, 470, 633
SatI GCNGC 4 cut(s) 90, 246, 371, 374
Sau3AI GATC 4 cut(s) 156, 307, 317, 434
SchI GAGTC 1 cut(s) 285
SduI GDGCHC 1 cut(s) 485
SfaNI GCATC 1 cut(s) 418
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 6 cut(s) 71, 462, 471, 506, 582, 629
SsiI CCGC 2 cut(s) 246, 376
SspMI CTAG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 120
TaiI ACGT 1 cut(s) 341
TaqI TCGA 1 cut(s) 289
TasI AATT 6 cut(s) 71, 462, 471, 506, 582, 629
TatI WGTACW 1 cut(s) 142
TauI GCSGC 1 cut(s) 248
TfiI GAWTC 2 cut(s) 227, 409
Tru1I TTAA 3 cut(s) 36, 470, 633
Tru9I TTAA 3 cut(s) 36, 470, 633
TseFI GTSAC 1 cut(s) 575
TseI GCWGC 3 cut(s) 89, 370, 373
Tsp45I GTSAC 1 cut(s) 575
TspDTI ATGAA 3 cut(s) 225, 474, 634
TspGWI ACGGA 2 cut(s) 244, 340
Van91I CCANNNNNTGG 1 cut(s) 129
XapI RAATTY 3 cut(s) 462, 506, 629
XceI RCATGY 1 cut(s) 596
XspI CTAG 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.