Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0002451.1

Orthogroup ID

OG0016303

Physical Location & Seq

Genomic Coordinates Reverse (-)

202149 .. 208107

Gene Structure 5959 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3732 bp

atggtttctgcttatttttcttcttccaatgtttcagccttgctcgttttcctcttcatgatgagcacttccatggttgcctttggtggcaaattttacgatgatttctacataacatggggaccggagaatgctaaaatgctcgattatgggcagctcttgactctatccctcaatagtgtcagtggctcggcatttgagtcctacaatcaatatctatatgtaaagatagacatgcaaatcaaacttgttgccggaaactctgccggtaccgtcactacattctacttatcctcaacaggaaatcatcatgatgagatagactttgaattcttgggaaatgttaccggtcagccttacattcttcatacgaatgtcttttgccaaggacagggcaacaaagagcagcaattctacctttggtttgatcctactgccgatttccacacatattctatcctttggaatcccctaaacattgtcttcttggtagatggcattcccattagacagttcaagaacctggagtcctttggcattccatttcccaaataccagcctatgaggttatactccagtctctggaatgcggataactgggctacaagaggtggactggagaaaatagattggacgcaagcaccctttactgcatcttacaggaacttcaatgccaattctgattcccatggagcatggtactggaagaaggtggattattcgggtaaagggcagatacaatgggtgcaaaataactatatgatctacaactactgcacagactcgaagcgattccccctaggtgctaagggccgtgtggttggacttgacatcagcggtgaatttatctcatcaagcattgacagttcaagcagtctcttccatcttcaacatcttcaaagcctaaatttggcctgcaatgacttcaatgcctctcaaattccagccgcaataggaaagcttacaaacttgaagtatctaaatttgtcgtctgcggaattcttcggccaaattcccattgagatttcaaaattgacaaggttggttatccttgacatatctgaaaatttctctattagaggtccattaagacttcagaaaccaaatttaagcatgctggttcagaacctcacacagcttacagagttatatcttgatgtcgttcaaatattagctgagggaattgagtggtgccaagccatagcctcctcattgccaaacctgagggtgttgagcttatctgattgtgcgctttcaggccctttccatgagtctcttgctgagcttcagtctctatctgttgttcgtttggacaataacagattctttgctcctgttccaggattctttgccaattttccaaacttgactaccttgagtctctgtaattctcagctgcagggaacatttccggatgagattcttcaggtaccttctcttcaagctattgatataaccaataatctagaacttcatggttctttgccacagttttccaagaacaattctctttggtccctagttgtaagagaaacgaatttttctggacttttgccagagtcaattggaaatctcaggcagctgtcaacgatagacatttctggttgtgatttcactggatcaatcccaaggtcagttgggaacctaacacaattagttcagttgaaaatgtcaggaaacaaatttgatggtccaattgattctattcactgggaaagccttattaatctgctatatcttgctttggaatctaatttactcaatgggagaattccaacatctatcttttctcctcccttgttggaggaactactactttcccataatcaattctcaggtcaagttccaaaaatttccaatatctcttcgaaatcactgaacaaccttgatttgagtagcaacaatttggaaggaccaatatctgcttctctcctatatttgcgagggcttcgacaacttaatctttcttcaaacaatttcagtggttttcctttcattggccctcaacagctcgaaaatgttaaagacattgatctttcgcacaatagcttattgatatgttcaaatgtcagcaattcctcatcttccacatttcctcaaattgaaatattgaacttgtcttctaacaagctgagaacattcccacatatgttgagaaatctctccagattgtaccatatggacctttcggaaaaccaaatgcaaggcaagattcccaaatggatatggggagtcagttctcttcaacacctaaatgtttcttgtaactccttagtgactgtagaagctccttcactcaattctacttcatttgtctctgagcttgaccttcatttgaaccaatttcagggacagatcccaattttcctaccatttgctacttatctggattactcaaggaatcatttcagctctagcgtaccaattgacattggtacttccattaattccatttctttcatctctctttcaagtaataagttatatgggacaattccagaatcaatatgcaacatctcgtcatatcttgaggttcttgatttatcccataattctctaagtggcttagttcccgaatgcttgactacaatggacatttccagtgtacttaatttaaggggaaacaacctcactggcactattcctgatacatttcttgaagattgcagtttaaaaacgctggagctaagtgaaaatcagatggaaggccaggttccaaaatctctagccagctgcacaagtttagaaacgttaaaccttggaagcaatcagattagcgatacctttccgtgcttattgatgaaaatatccaccttgagtgtgcttgttttgcggtccaacaaatttcatggaggcattgaatgttcagagatctatggcacctggcagaggcttcaagtgatagacctagcacacaacttcttcagtggtggaatatcaggaatatctttaccaagttcgctgaaaaaaatctctaaaatatataacatatataggtctttattttggcaactcaacgatttgccaccagaaactattggtcatgagtatgttgaaaagtatttcgaggaagcaataacagttaccagcaaaggtttaaacatgaagctggtgaaaattatggcagccttcaccttgattgacttatcaaacaacaaattcaacgggtcaatcccagagtacattgaaatatacgaatcattacaggtgttcaacttgtccaataatgatttcacaggtgaaatcccatcttcatttggtgatatgcttcaactagaatccttagacctctcacaaaaccacttgagtgggaaaattccacaaaagcttgctgaacttactttcctgtcattcctgaatctctccaataatcaactggtcggcaaaatcccaaccggtccgcagttttcaacattttcaaaagcctcatttttaggtaacaaaggattatggggttttcctttggacaacatggtagtattgtctccaccaagatcacatggaatccctccaaaatctgcacataagattgactgggatcttatcgatattgaaattggatttgtatttgggcttggaattgccatcgggtcacttttcttttgcaagagatggaggaaatggtattacagagttatgtataacattgtgcttatgatattccctcagctggatcaaagaattggtaatcatagaagacatgtttacattgctccaagatggagacgttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005794 GO:0005975 GO:0005976 GO:0006073 GO:0006950 GO:0008150 GO:0008152 GO:0009266 GO:0009408 GO:0009409 GO:0009605 GO:0009612 GO:0009628 GO:0009664 GO:0009719 GO:0009725 GO:0009733 GO:0009741 GO:0009987 GO:0010033 GO:0010383 GO:0010410 GO:0010411 GO:0012505 GO:0014070 GO:0016043 GO:0016740 GO:0016757 GO:0016758 GO:0016762 GO:0030312 GO:0033993 GO:0042221 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044036 GO:0044042 GO:0044237 GO:0044238 GO:0044260 GO:0044262 GO:0044264 GO:0044424 GO:0044444 GO:0044464 GO:0045229 GO:0046527 GO:0050896 GO:0071554 GO:0071555 GO:0071669 GO:0071704 GO:0071840 GO:0071944 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko04075 map04075

Pfam Domains

Protein Families

Glyco_hydro_16 XET_C

Protein Analysis

1243

Amino Acids

139.18

Weight (kDa)

5.83

Isoelectric Point (pI)

41.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_16	PF00722	33 - 212	8.6e-62	Glycosyl hydrolases family 16
XET_C	PF06955	231 - 267	2.2e-13	Xyloglucan endo-transglycosylase (XET) C-terminus
LRR_14	PF23598	289 - 486	3.5e-12	Leucine-rich repeat region
LRR_8	PF13855	431 - 488	6.5e-07	Leucine rich repeat
LRR_14	PF23598	507 - 559	2.5e-06	Leucine-rich repeat region
LRR_8	PF13855	699 - 758	1.7e-07	Leucine rich repeat
LRR_8	PF13855	869 - 925	5.9e-08	Leucine rich repeat
LRR_4	PF12799	890 - 927	3.5e-06	Leucine Rich repeats (2 copies)
LRR_8	PF13855	1067 - 1126	2e-07	Leucine rich repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0016303)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_4g09250
malus_domestica	MD13G1268400.v1.1
prunus_persica	Prupe.1G088500_v2.0.a1
rosa_chinensis	RchiOBHm_Chr4g0402841 RchiOBHm_Chr4g0402851 RchiOBHm_Chr4g0402861
rosa_laevigata	RLG00000009045
rosa_multiflora	Rmu_sc0002451.1_g000049 Rmu_sc0008058.1_g000013
rosa_roxburghii	Rroxscaffold_5G00347580 Rroxscaffold_5G00347590
rosa_rugosa	Rorug04G0038700 Rorug04G0038800
rosa_samantha	Rh4AG116100
rosa_wichuraiana	Rw4G009300

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	2 cut(s)	269, 1429
AccB1I	GGYRCC	4 cut(s)	269, 1191, 1429, 2879
AccB7I	CCANNNNNTGG	1 cut(s)	582
AccIII	TCCGGA	1 cut(s)	1411
AciI	CCGC	6 cut(s)	590, 837, 948, 995, 2833, 3401
AclI	AACGTT	1 cut(s)	2750
AclWI	GGATC	5 cut(s)	422, 1627, 2350, 3546, 3681
AcoI	YGGCCR	1 cut(s)	1006
AcuI	CTGAAG	4 cut(s)	1079, 1271, 1409, 2908
AfaI	GTAC	8 cut(s)	271, 701, 1431, 2174, 2421, 2437, 2607, 3182
AfiI	CCNNNNNNNGG	8 cut(s)	391, 582, 910, 1340, 1997, 2347, 2889, 3670
AflIII	ACRYGT	1 cut(s)	3700
AgeI	ACCGGT	2 cut(s)	347, 3395
AjnI	CCWGG	4 cut(s)	522, 1339, 2709, 2882
AjuI	GAANNNNNNNTTGG	4 cut(s)	1909, 1941, 3215, 3247
AleI	CACNNNNGTG	1 cut(s)	3306
AloI	GAACNNNNNNTCC	4 cut(s)	512, 544, 3327, 3359
Alw21I	GWGCWC	1 cut(s)	68
Alw26I	GTCTC	8 cut(s)	584, 881, 1278, 1296, 1385, 2320, 3489, 3718
AlwI	GGATC	5 cut(s)	422, 1627, 2350, 3546, 3681
AlwNI	CAGNNNCTG	1 cut(s)	582
Aor13HI	TCCGGA	1 cut(s)	1411
AoxI	GGCC	6 cut(s)	811, 912, 1006, 1258, 1999, 2707
ApeKI	GCWGC	6 cut(s)	154, 406, 1396, 1579, 2733, 3125
AseI	ATTAAT	2 cut(s)	1725, 2445
AsiGI	ACCGGT	2 cut(s)	347, 3395
Asp700I	GAANNNNTTC	2 cut(s)	791, 2405
Asp718I	GGTACC	2 cut(s)	269, 1429
AspA2I	CCTAGG	1 cut(s)	799
AspLEI	GCGC	1 cut(s)	1252
AsuHPI	GGTGA	5 cut(s)	851, 3124, 3124, 3251, 3272
AsuII	TTCGAA	1 cut(s)	1868
AvaII	GGWCC	8 cut(s)	122, 1082, 1515, 1691, 1913, 2182, 2835, 3398
AvrII	CCTAGG	1 cut(s)	799
AxyI	CCTNAGG	1 cut(s)	1223
BanI	GGYRCC	4 cut(s)	269, 1191, 1429, 2879
BarI	GAAGNNNNNNTAC	2 cut(s)	1422, 1454
BbsI	GAAGAC	3 cut(s)	475, 2112, 3703
Bbv12I	GWGCWC	1 cut(s)	68
BbvCI	CCTCAGC	2 cut(s)	1176, 3666
BbvI	GCAGC	6 cut(s)	166, 418, 1383, 1591, 2720, 3137
BccI	CCATC	8 cut(s)	488, 891, 1682, 2695, 3256, 3593, 3606, 3714
BceAI	ACGGC	1 cut(s)	798
BciT130I	CCWGG	4 cut(s)	524, 1341, 2711, 2884
BcoDI	GTCTC	8 cut(s)	584, 881, 1278, 1296, 1385, 2320, 3489, 3718
BfaI	CTAG	7 cut(s)	800, 1466, 1520, 2415, 2726, 2909, 3275
BfmI	CTRYAG	2 cut(s)	1397, 2280
BglII	AGATCT	1 cut(s)	2871
BisI	GCNGC	7 cut(s)	155, 407, 948, 1397, 1580, 2734, 3126
BlnI	CCTAGG	1 cut(s)	799
BlpI	GCTNAGC	1 cut(s)	1281
BlsI	GCNGC	7 cut(s)	156, 408, 949, 1398, 1581, 2735, 3127
Bme1390I	CCNGG	4 cut(s)	524, 1341, 2711, 2884
Bme18I	GGWCC	8 cut(s)	122, 1082, 1515, 1691, 1913, 2182, 2835, 3398
BmiI	GGNNCC	8 cut(s)	123, 271, 1193, 1431, 1517, 1643, 2715, 2881
BmrFI	CCNGG	4 cut(s)	524, 1341, 2711, 2884
BmrI	ACTGGG	3 cut(s)	607, 1720, 3544
BmsI	GCATC	1 cut(s)	662
BmuI	ACTGGG	3 cut(s)	607, 1720, 3544
BpiI	GAAGAC	3 cut(s)	475, 2112, 3703
BpmI	CTGGAG	5 cut(s)	545, 559, 638, 2149, 2702
Bpu10I	CCTNAGC	3 cut(s)	807, 1176, 3666
Bpu1102I	GCTNAGC	1 cut(s)	1281
Bpu14I	TTCGAA	1 cut(s)	1868
BpuEI	CTTGAG	5 cut(s)	1396, 2380, 2549, 2836, 3325
Bsa29I	ATCGAT	1 cut(s)	3546
BsaJI	CCNNGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
BsaWI	WCCGGW	4 cut(s)	124, 347, 1411, 3395
Bsc4I	CCNNNNNNNGG	8 cut(s)	391, 582, 910, 1340, 1997, 2347, 2889, 3670
Bse118I	RCCGGY	3 cut(s)	266, 347, 3395
Bse21I	CCTNAGG	1 cut(s)	1223
Bse3DI	GCAATG	3 cut(s)	925, 1211, 3708
BseAI	TCCGGA	1 cut(s)	1411
BseBI	CCWGG	4 cut(s)	524, 1341, 2711, 2884
BseCI	ATCGAT	1 cut(s)	3546
BseDI	CCNNGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
BseGI	GGATG	1 cut(s)	1420
BseLI	CCNNNNNNNGG	8 cut(s)	391, 582, 910, 1340, 1997, 2347, 2889, 3670
BseMI	GCAATG	3 cut(s)	925, 1211, 3708
BseMII	CTCAG	9 cut(s)	1167, 1214, 1272, 1406, 1588, 1848, 2123, 2310, 3680
BseRI	GAGGAG	2 cut(s)	1198, 1782
BseXI	GCAGC	6 cut(s)	166, 418, 1383, 1591, 2720, 3137
BsgI	GTGCAG	3 cut(s)	760, 2719, 3504
BshFI	GGCC	6 cut(s)	813, 914, 1008, 1260, 2001, 2709
BshNI	GGYRCC	4 cut(s)	269, 1191, 1429, 2879
BshTI	ACCGGT	2 cut(s)	347, 3395
BshVI	ATCGAT	1 cut(s)	3546
BsiHKAI	GWGCWC	1 cut(s)	68
BsiSI	CCGG	6 cut(s)	125, 255, 267, 348, 1412, 3396
BslFI	GGGAC	4 cut(s)	135, 1501, 2364, 2503
BslI	CCNNNNNNNGG	8 cut(s)	391, 582, 910, 1340, 1997, 2347, 2889, 3670
BsmAI	GTCTC	8 cut(s)	584, 881, 1278, 1296, 1385, 2320, 3489, 3718
BsmBI	CGTCTC	1 cut(s)	3718
BsmFI	GGGAC	4 cut(s)	135, 1501, 2364, 2503
BsmI	GAATGC	5 cut(s)	136, 498, 537, 592, 2582
BsnI	GGCC	6 cut(s)	813, 914, 1008, 1260, 2001, 2709
Bsp119I	TTCGAA	1 cut(s)	1868
Bsp1286I	GDGCHC	1 cut(s)	68
Bsp13I	TCCGGA	1 cut(s)	1411
Bsp143I	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
Bsp1720I	GCTNAGC	1 cut(s)	1281
Bsp19I	CCATGG	2 cut(s)	72, 688
BspACI	CCGC	6 cut(s)	590, 837, 948, 995, 2833, 3401
BspANI	GGCC	6 cut(s)	813, 914, 1008, 1260, 2001, 2709
BspCNI	CTCAG	9 cut(s)	1168, 1215, 1273, 1405, 1587, 1847, 2124, 2311, 3679
BspDI	ATCGAT	1 cut(s)	3546
BspEI	TCCGGA	1 cut(s)	1411
BspHI	TCATGA	3 cut(s)	57, 310, 3043
BspLI	GGNNCC	8 cut(s)	123, 271, 1193, 1431, 1517, 1643, 2715, 2881
BspMAI	CTGCAG	1 cut(s)	1401
BspPI	GGATC	5 cut(s)	422, 1627, 2350, 3546, 3681
BspT104I	TTCGAA	1 cut(s)	1868
BspT107I	GGYRCC	4 cut(s)	269, 1191, 1429, 2879
BsrDI	GCAATG	3 cut(s)	925, 1211, 3708
BsrFI	RCCGGY	3 cut(s)	266, 347, 3395
BssAI	RCCGGY	3 cut(s)	266, 347, 3395
BssECI	CCNNGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
BssMI	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
BssT1I	CCWWGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
Bst2UI	CCWGG	4 cut(s)	524, 1341, 2711, 2884
Bst4CI	ACNGT	6 cut(s)	274, 513, 866, 1491, 2281, 3082
Bst6I	CTCTTC	5 cut(s)	59, 884, 1443, 1870, 2247
BstBI	TTCGAA	1 cut(s)	1868
BstC8I	GCNNGC	5 cut(s)	639, 916, 1115, 2731, 3330
BstDSI	CCRYGG	2 cut(s)	72, 688
BstENI	CCTNNNNNAGG	2 cut(s)	1338, 2887
BstF5I	GGATG	1 cut(s)	1420
BstHHI	GCGC	1 cut(s)	1252
BstKTI	GATC	9 cut(s)	430, 765, 1622, 2035, 2358, 2874, 3497, 3541, 3676
BstMAI	GTCTC	8 cut(s)	584, 881, 1278, 1296, 1385, 2320, 3489, 3718
BstMBI	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
BstMWI	GCNNNNNNNGC	1 cut(s)	2830
BstNI	CCWGG	4 cut(s)	524, 1341, 2711, 2884
BstNSI	RCATGY	3 cut(s)	238, 1117, 3704
BstSCI	CCNGG	4 cut(s)	522, 1339, 2709, 2882
BstSFI	CTRYAG	2 cut(s)	1397, 2280
BstV1I	GCAGC	6 cut(s)	166, 418, 1383, 1591, 2720, 3137
BstV2I	GAAGAC	3 cut(s)	475, 2112, 3703
BstX2I	RGATCY	3 cut(s)	2355, 2871, 3538
BstXI	CCANNNNNNTGG	1 cut(s)	3308
BstYI	RGATCY	3 cut(s)	2355, 2871, 3538
Bsu15I	ATCGAT	1 cut(s)	3546
Bsu36I	CCTNAGG	1 cut(s)	1223
BsuRI	GGCC	6 cut(s)	813, 914, 1008, 1260, 2001, 2709
BsuTUI	ATCGAT	1 cut(s)	3546
BtgI	CCRYGG	2 cut(s)	72, 688
BtsCI	GGATG	1 cut(s)	1420
BtsIMutI	CAGTG	8 cut(s)	190, 1614, 1708, 1874, 1987, 2608, 2631, 2932
Cac8I	GCNNGC	5 cut(s)	639, 916, 1115, 2731, 3330
CaiI	CAGNNNCTG	1 cut(s)	582
CciI	TCATGA	3 cut(s)	57, 310, 3043
CfoI	GCGC	1 cut(s)	1252
Cfr10I	RCCGGY	3 cut(s)	266, 347, 3395
ClaI	ATCGAT	1 cut(s)	3546
CpoI	CGGWCCG	1 cut(s)	3398
CseI	GACGC	1 cut(s)	643
Csp6I	GTAC	8 cut(s)	270, 700, 1430, 2173, 2420, 2436, 2606, 3181
CspAI	ACCGGT	2 cut(s)	347, 3395
CspCI	CAANNNNNGTGG	4 cut(s)	1963, 1998, 2133, 2168
CspI	CGGWCCG	1 cut(s)	3398
CviQI	GTAC	8 cut(s)	270, 700, 1430, 2173, 2420, 2436, 2606, 3181
DpnI	GATC	9 cut(s)	429, 764, 1621, 2034, 2357, 2873, 3496, 3540, 3675
DpnII	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
DraI	TTTAAA	2 cut(s)	2673, 3099
EaeI	YGGCCR	1 cut(s)	1006
Eam1104I	CTCTTC	5 cut(s)	59, 884, 1443, 1870, 2247
EarI	CTCTTC	5 cut(s)	59, 884, 1443, 1870, 2247
Eco130I	CCWWGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
Eco47I	GGWCC	8 cut(s)	122, 1082, 1515, 1691, 1913, 2182, 2835, 3398
Eco57I	CTGAAG	4 cut(s)	1079, 1271, 1409, 2908
Eco81I	CCTNAGG	1 cut(s)	1223
EcoNI	CCTNNNNNAGG	2 cut(s)	1338, 2887
EcoO109I	RGGNCCY	1 cut(s)	1259
EcoRI	GAATTC	3 cut(s)	329, 998, 1770
EcoRII	CCWGG	4 cut(s)	522, 1339, 2709, 2882
EcoT14I	CCWWGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
ErhI	CCWWGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
Esp3I	CGTCTC	1 cut(s)	3718
FaqI	GGGAC	4 cut(s)	135, 1501, 2364, 2503
FauNDI	CATATG	2 cut(s)	2148, 2178
Fnu4HI	GCNGC	7 cut(s)	155, 407, 948, 1397, 1580, 2734, 3126
FokI	GGATG	1 cut(s)	1427
Fsp4HI	GCNGC	7 cut(s)	155, 407, 948, 1397, 1580, 2734, 3126
FspBI	CTAG	7 cut(s)	800, 1466, 1520, 2415, 2726, 2909, 3275
GlaI	GCGC	1 cut(s)	1251
GluI	GCNGC	7 cut(s)	155, 407, 948, 1397, 1580, 2734, 3126
GsuI	CTGGAG	5 cut(s)	545, 559, 638, 2149, 2702
HaeIII	GGCC	6 cut(s)	813, 914, 1008, 1260, 2001, 2709
HapII	CCGG	6 cut(s)	125, 255, 267, 348, 1412, 3396
HgaI	GACGC	1 cut(s)	643
HhaI	GCGC	1 cut(s)	1252
Hin6I	GCGC	1 cut(s)	1250
HinP1I	GCGC	1 cut(s)	1250
HincII	GTYRAC	1 cut(s)	1587
HindII	GTYRAC	1 cut(s)	1587
HindIII	AAGCTT	2 cut(s)	959, 3326
HpaII	CCGG	6 cut(s)	125, 255, 267, 348, 1412, 3396
HphI	GGTGA	5 cut(s)	851, 3124, 3124, 3251, 3272
Hpy166II	GTNNAC	4 cut(s)	614, 1587, 2606, 3706
Hpy8I	GTNNAC	4 cut(s)	614, 1587, 2606, 3706
HpyAV	CCTTC	7 cut(s)	703, 1443, 1904, 2301, 2339, 2699, 3139
HpyCH4III	ACNGT	6 cut(s)	274, 513, 866, 1491, 2281, 3082
HpyCH4IV	ACGT	2 cut(s)	2750, 3727
HpyF10VI	GCNNNNNNNGC	1 cut(s)	2830
HpySE526I	ACGT	2 cut(s)	2750, 3727
HspAI	GCGC	1 cut(s)	1250
Kpn2I	TCCGGA	1 cut(s)	1411
KpnI	GGTACC	2 cut(s)	273, 1433
Kzo9I	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
LmnI	GCTCC	5 cut(s)	692, 1336, 2293, 2683, 3718
Lsp1109I	GCAGC	6 cut(s)	166, 418, 1383, 1591, 2720, 3137
LweI	GCATC	1 cut(s)	662
MaeI	CTAG	7 cut(s)	800, 1466, 1520, 2415, 2726, 2909, 3275
MaeII	ACGT	2 cut(s)	2750, 3727
MaeIII	GTNAC	7 cut(s)	274, 343, 2264, 2275, 3082, 3437, 3591
MalI	GATC	9 cut(s)	429, 764, 1621, 2034, 2357, 2873, 3496, 3540, 3675
MboI	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
MfeI	CAATTG	3 cut(s)	1563, 1695, 2424
MflI	RGATCY	3 cut(s)	2355, 2871, 3538
MhlI	GDGCHC	1 cut(s)	68
MlyI	GAGTC	8 cut(s)	157, 209, 536, 776, 1280, 1387, 1568, 2241
MmeI	TCCRAC	4 cut(s)	802, 1782, 1799, 2862
MroI	TCCGGA	1 cut(s)	1411
MroXI	GAANNNNTTC	2 cut(s)	791, 2405
MslI	CAYNNNNRTG	6 cut(s)	71, 312, 372, 2253, 3048, 3306
MspA1I	CMGCKG	5 cut(s)	837, 1396, 1582, 2733, 3670
MspI	CCGG	6 cut(s)	125, 255, 267, 348, 1412, 3396
MspR9I	CCNGG	4 cut(s)	524, 1341, 2711, 2884
MssI	GTTTAAAC	1 cut(s)	3099
MunI	CAATTG	3 cut(s)	1563, 1695, 2424
Mva1269I	GAATGC	5 cut(s)	136, 498, 537, 592, 2582
MvaI	CCWGG	4 cut(s)	524, 1341, 2711, 2884
MwoI	GCNNNNNNNGC	1 cut(s)	2830
NcoI	CCATGG	2 cut(s)	72, 688
NdeI	CATATG	2 cut(s)	2148, 2178
NdeII	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
NlaIV	GGNNCC	8 cut(s)	123, 271, 1193, 1431, 1517, 1643, 2715, 2881
NmeAIII	GCCGAG	1 cut(s)	170
NmuCI	GTSAC	3 cut(s)	274, 2275, 3591
NspI	RCATGY	3 cut(s)	238, 1117, 3704
NspV	TTCGAA	1 cut(s)	1868
OliI	CACNNNNGTG	1 cut(s)	3306
PaeI	GCATGC	1 cut(s)	1117
PagI	TCATGA	3 cut(s)	57, 310, 3043
PciI	ACATGT	1 cut(s)	3700
PctI	GAATGC	5 cut(s)	136, 498, 537, 592, 2582
PdmI	GAANNNNTTC	2 cut(s)	791, 2405
PflMI	CCANNNNNTGG	1 cut(s)	582
PfoI	TCCNGGA	1 cut(s)	1339
PinAI	ACCGGT	2 cut(s)	347, 3395
PkrI	GCNGC	7 cut(s)	156, 408, 949, 1398, 1581, 2735, 3127
PleI	GAGTC	8 cut(s)	157, 208, 535, 776, 1279, 1386, 1567, 2240
PmeI	GTTTAAAC	1 cut(s)	3099
PpsI	GAGTC	8 cut(s)	157, 208, 535, 776, 1279, 1386, 1567, 2240
PscI	ACATGT	1 cut(s)	3700
PshBI	ATTAAT	2 cut(s)	1725, 2445
Psp1406I	AACGTT	1 cut(s)	2750
Psp6I	CCWGG	4 cut(s)	522, 1339, 2709, 2882
PspGI	CCWGG	4 cut(s)	522, 1339, 2709, 2882
PspN4I	GGNNCC	8 cut(s)	123, 271, 1193, 1431, 1517, 1643, 2715, 2881
PstI	CTGCAG	1 cut(s)	1401
PstNI	CAGNNNCTG	1 cut(s)	582
PsuI	RGATCY	3 cut(s)	2355, 2871, 3538
PvuII	CAGCTG	4 cut(s)	1396, 1582, 2733, 3670
RsaI	GTAC	8 cut(s)	271, 701, 1431, 2174, 2421, 2437, 2607, 3182
RsaNI	GTAC	8 cut(s)	270, 700, 1430, 2173, 2420, 2436, 2606, 3181
RseI	CAYNNNNRTG	6 cut(s)	71, 312, 372, 2253, 3048, 3306
Rsr2I	CGGWCCG	1 cut(s)	3398
RsrII	CGGWCCG	1 cut(s)	3398
SatI	GCNGC	7 cut(s)	155, 407, 948, 1397, 1580, 2734, 3126
Sau3AI	GATC	9 cut(s)	427, 762, 1619, 2032, 2355, 2871, 3494, 3538, 3673
SchI	GAGTC	8 cut(s)	157, 209, 536, 776, 1280, 1387, 1568, 2241
ScrFI	CCNGG	4 cut(s)	524, 1341, 2711, 2884
SduI	GDGCHC	1 cut(s)	68
SfaNI	GCATC	1 cut(s)	662
SfcI	CTRYAG	2 cut(s)	1397, 2280
SfuI	TTCGAA	1 cut(s)	1868
SinI	GGWCC	8 cut(s)	122, 1082, 1515, 1691, 1913, 2182, 2835, 3398
SmiMI	CAYNNNNRTG	6 cut(s)	71, 312, 372, 2253, 3048, 3306
SmlI	CTYRAG	5 cut(s)	1375, 2395, 2528, 2815, 3304
SmoI	CTYRAG	5 cut(s)	1375, 2395, 2528, 2815, 3304
SphI	GCATGC	1 cut(s)	1117
SsiI	CCGC	6 cut(s)	590, 837, 948, 995, 2833, 3401
SspI	AATATT	2 cut(s)	1170, 2109
SspMI	CTAG	7 cut(s)	800, 1466, 1520, 2415, 2726, 2909, 3275
StyD4I	CCNGG	4 cut(s)	522, 1339, 2709, 2882
StyI	CCWWGG	6 cut(s)	72, 385, 688, 799, 1628, 2758
TaaI	ACNGT	6 cut(s)	274, 513, 866, 1491, 2281, 3082
TaiI	ACGT	2 cut(s)	2753, 3730
TaqI	TCGA	7 cut(s)	144, 785, 1868, 1951, 2013, 3066, 3546
TatI	WGTACW	2 cut(s)	2605, 3180
TauI	GCSGC	1 cut(s)	950
TscAI	CASTG	8 cut(s)	190, 1621, 1715, 1881, 1987, 2608, 2638, 2932
TseFI	GTSAC	3 cut(s)	274, 2275, 3591
TseI	GCWGC	6 cut(s)	154, 406, 1396, 1579, 2733, 3125
Tsp45I	GTSAC	3 cut(s)	274, 2275, 3591
TspGWI	ACGGA	1 cut(s)	2778
TspRI	CASTG	8 cut(s)	190, 1621, 1715, 1881, 1987, 2608, 2638, 2932
Van91I	CCANNNNNTGG	1 cut(s)	582
VpaK11BI	GGWCC	8 cut(s)	122, 1082, 1515, 1691, 1913, 2182, 2835, 3398
VspI	ATTAAT	2 cut(s)	1725, 2445
XagI	CCTNNNNNAGG	2 cut(s)	1338, 2887
XbaI	TCTAGA	1 cut(s)	1465
XceI	RCATGY	3 cut(s)	238, 1117, 3704
XcmI	CCANNNNNNNNNTGG	1 cut(s)	3373
XmaJI	CCTAGG	1 cut(s)	799
XmnI	GAANNNNTTC	2 cut(s)	791, 2405
XspI	CTAG	7 cut(s)	800, 1466, 1520, 2415, 2726, 2909, 3275

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.