Rmu_sc0002541.1_g000010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002541.1
OG0018630
Physical Location & Seq
Reverse (-)
44131 .. 46149
2019 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002541.1_g000010.1.cds

Sequence Viewer

Length: 705 bp
atgcattctcctattgtgtttgacccttacctaggcccacagcagaacgaacctccttttgtcaccaacattggtggcaagaagccaaactcagtccctcaatccaccctcaatccctttcaccctctcaagctcacacacactctcatcctcctgcgattccggcaaagcaagtttgggtatggaagttttgggtatagaagtttgggtaaagtaagtttgcaaagcatagacgtcctcaagtttggttcgatttctggttcgatttcgaattcttcaagcttgggatctatcaaagaatcaactgactggctcattaccagacttggggagcttgcgaaaccttcgtacaacttgctggaaggcgctataccaccaaccttgggttcactagtgaatcttcggcaactgtatctctacttcaataggttatcaggctcaattcctgatgaattgggaaatctcagcaatcttactacattggatatttctgagaaccagctcactggtccgcttcccaaaacacttggaaatttgaaatccctcagcggcttctgggccacagccaattccttgaatgataaatttccggacacttatgggaaccttacgagtctaaaatatttttcaatatccgggaattatatctctggtccattcccagttgacatcataaagaactggactaatattgaaactctgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

234

Amino Acids

25.66

Weight (kDa)

9.2

Isoelectric Point (pI)

34.04

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 237
AccIII TCCGGA 1 cut(s) 589
AciI CCGC 2 cut(s) 512, 549
AclWI GGATC 1 cut(s) 295
AcsI RAATTY 3 cut(s) 271, 532, 584
AcyI GRCGYC 1 cut(s) 234
AfaI GTAC 1 cut(s) 350
AfiI CCNNNNNNNGG 3 cut(s) 32, 327, 383
AgsI TTSAA 6 cut(s) 279, 424, 538, 577, 630, 695
AhlI ACTAGT 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 370, 402
AluBI AGCT 4 cut(s) 133, 282, 334, 502
AluI AGCT 4 cut(s) 133, 282, 334, 502
AlwI GGATC 1 cut(s) 295
Aor13HI TCCGGA 1 cut(s) 589
AoxI GGCC 2 cut(s) 34, 558
ApoI RAATTY 3 cut(s) 271, 532, 584
AspA2I CCTAGG 1 cut(s) 31
AspLEI GCGC 1 cut(s) 368
AspS9I GGNCC 4 cut(s) 35, 509, 558, 653
AsuC2I CCSGG 1 cut(s) 637
AsuHPI GGTGA 2 cut(s) 55, 113
AsuII TTCGAA 1 cut(s) 269
AvaII GGWCC 2 cut(s) 509, 653
AvrII CCTAGG 1 cut(s) 31
BarI GAAGNNNNNNTAC 4 cut(s) 354, 386, 404, 436
BbvCI CCTCAGC 1 cut(s) 545
BcnI CCSGG 1 cut(s) 637
BcuI ACTAGT 1 cut(s) 391
BfaI CTAG 2 cut(s) 32, 392
BfoI RGCGCY 1 cut(s) 369
BisI GCNGC 1 cut(s) 550
BlnI CCTAGG 1 cut(s) 31
BlsI GCNGC 1 cut(s) 551
Bme1390I CCNGG 1 cut(s) 637
Bme18I GGWCC 2 cut(s) 509, 653
BmgT120I GGNCC 4 cut(s) 35, 509, 558, 653
BmiI GGNNCC 1 cut(s) 605
BmrFI CCNGG 1 cut(s) 637
BmrI ACTGGG 1 cut(s) 656
BmuI ACTGGG 1 cut(s) 656
Bpu10I CCTNAGC 1 cut(s) 545
Bpu14I TTCGAA 1 cut(s) 269
BpuEI CTTGAG 2 cut(s) 113, 224
BpuMI CCSGG 1 cut(s) 637
BsaHI GRCGYC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 31, 381
BsaWI WCCGGW 1 cut(s) 589
Bsc4I CCNNNNNNNGG 3 cut(s) 32, 327, 383
Bse1I ACTGG 4 cut(s) 314, 511, 662, 686
BseAI TCCGGA 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 31, 381
BseGI GGATG 1 cut(s) 147
BseLI CCNNNNNNNGG 3 cut(s) 32, 327, 383
BseMII CTCAG 4 cut(s) 105, 478, 483, 559
BseNI ACTGG 4 cut(s) 314, 511, 662, 686
BshFI GGCC 2 cut(s) 36, 560
BsiSI CCGG 3 cut(s) 163, 590, 636
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 32, 327, 383
BsmFI GGGAC 1 cut(s) 80
BsmI GAATGC 1 cut(s) 4
BsnI GGCC 2 cut(s) 36, 560
Bsp119I TTCGAA 1 cut(s) 269
Bsp13I TCCGGA 1 cut(s) 589
Bsp143I GATC 1 cut(s) 287
BspACI CCGC 2 cut(s) 512, 549
BspANI GGCC 2 cut(s) 36, 560
BspCNI CTCAG 4 cut(s) 104, 477, 484, 558
BspEI TCCGGA 1 cut(s) 589
BspLI GGNNCC 1 cut(s) 605
BspPI GGATC 1 cut(s) 295
BspT104I TTCGAA 1 cut(s) 269
BsrI ACTGG 4 cut(s) 314, 511, 662, 686
BssECI CCNNGG 2 cut(s) 31, 381
BssMI GATC 1 cut(s) 287
BssNI GRCGYC 1 cut(s) 234
BssT1I CCWWGG 2 cut(s) 31, 381
Bst4CI ACNGT 1 cut(s) 411
BstACI GRCGYC 1 cut(s) 234
BstBI TTCGAA 1 cut(s) 269
BstC8I GCNNGC 1 cut(s) 336
BstDEI CTNAG 4 cut(s) 91, 464, 492, 545
BstENI CCTNNNNNAGG 1 cut(s) 30
BstF5I GGATG 1 cut(s) 147
BstH2I RGCGCY 1 cut(s) 369
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 1 cut(s) 290
BstMBI GATC 1 cut(s) 287
BstMWI GCNNNNNNNGC 1 cut(s) 163
BstSCI CCNGG 1 cut(s) 635
BstX2I RGATCY 1 cut(s) 287
BstXI CCANNNNNNTGG 1 cut(s) 506
BstYI RGATCY 1 cut(s) 287
BsuRI GGCC 2 cut(s) 36, 560
BtsCI GGATG 1 cut(s) 147
BtsIMutI CAGTG 1 cut(s) 504
Cac8I GCNNGC 1 cut(s) 336
CfoI GCGC 1 cut(s) 368
Cfr13I GGNCC 4 cut(s) 35, 509, 558, 653
Csp6I GTAC 1 cut(s) 349
CspCI CAANNNNNGTGG 4 cut(s) 55, 90, 363, 398
CviQI GTAC 1 cut(s) 349
DdeI CTNAG 4 cut(s) 91, 464, 492, 545
DpnI GATC 1 cut(s) 289
DpnII GATC 1 cut(s) 287
Eco130I CCWWGG 2 cut(s) 31, 381
Eco47I GGWCC 2 cut(s) 509, 653
EcoNI CCTNNNNNAGG 1 cut(s) 30
EcoRI GAATTC 1 cut(s) 271
EcoT14I CCWWGG 2 cut(s) 31, 381
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 31, 381
FaiI YATR 7 cut(s) 183, 198, 230, 371, 600, 645, 674
FaqI GGGAC 1 cut(s) 80
Fnu4HI GCNGC 1 cut(s) 550
FokI GGATG 1 cut(s) 134
Fsp4HI GCNGC 1 cut(s) 550
FspBI CTAG 2 cut(s) 32, 392
GlaI GCGC 1 cut(s) 367
GluI GCNGC 1 cut(s) 550
HaeII RGCGCY 1 cut(s) 369
HaeIII GGCC 2 cut(s) 36, 560
HapII CCGG 3 cut(s) 163, 590, 636
HhaI GCGC 1 cut(s) 368
Hin1I GRCGYC 1 cut(s) 234
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HincII GTYRAC 1 cut(s) 667
HindII GTYRAC 1 cut(s) 667
HindIII AAGCTT 1 cut(s) 280
HinfI GANTC 4 cut(s) 159, 299, 397, 613
HpaII CCGG 3 cut(s) 163, 590, 636
HphI GGTGA 2 cut(s) 55, 113
Hpy166II GTNNAC 2 cut(s) 389, 667
Hpy188I TCNGA 1 cut(s) 493
Hpy188III TCNNGA 2 cut(s) 446, 590
Hpy8I GTNNAC 2 cut(s) 389, 667
HpyAV CCTTC 2 cut(s) 354, 356
HpyCH4III ACNGT 1 cut(s) 411
HpyCH4IV ACGT 1 cut(s) 234
HpyCH4V TGCA 2 cut(s) 4, 223
HpyF10VI GCNNNNNNNGC 1 cut(s) 163
HpyF3I CTNAG 4 cut(s) 91, 464, 492, 545
HpySE526I ACGT 1 cut(s) 234
Hsp92I GRCGYC 1 cut(s) 234
HspAI GCGC 1 cut(s) 366
Kpn2I TCCGGA 1 cut(s) 589
Kzo9I GATC 1 cut(s) 287
LmnI GCTCC 1 cut(s) 331
MaeI CTAG 2 cut(s) 32, 392
MaeII ACGT 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 61
MalI GATC 1 cut(s) 289
MboI GATC 1 cut(s) 287
MboII GAAGA 2 cut(s) 267, 392
MflI RGATCY 1 cut(s) 287
MluCI AATT 7 cut(s) 271, 441, 452, 532, 568, 584, 640
MlyI GAGTC 1 cut(s) 622
MnlI CCTC 7 cut(s) 63, 108, 119, 135, 161, 248, 554
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 589
MspA1I CMGCKG 1 cut(s) 549
MspI CCGG 3 cut(s) 163, 590, 636
MspR9I CCNGG 1 cut(s) 637
Mva1269I GAATGC 1 cut(s) 4
MwoI GCNNNNNNNGC 1 cut(s) 163
NciI CCSGG 1 cut(s) 637
NdeII GATC 1 cut(s) 287
NlaIV GGNNCC 1 cut(s) 605
NmuCI GTSAC 1 cut(s) 61
NsiI ATGCAT 1 cut(s) 6
NspV TTCGAA 1 cut(s) 269
PctI GAATGC 1 cut(s) 4
PfeI GAWTC 3 cut(s) 159, 299, 397
PfoI TCCNGGA 1 cut(s) 635
PkrI GCNGC 1 cut(s) 551
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
PspN4I GGNNCC 1 cut(s) 605
PspPI GGNCC 4 cut(s) 35, 509, 558, 653
PsuI RGATCY 1 cut(s) 287
RsaI GTAC 1 cut(s) 350
RsaNI GTAC 1 cut(s) 349
SatI GCNGC 1 cut(s) 550
Sau3AI GATC 1 cut(s) 287
Sau96I GGNCC 4 cut(s) 35, 509, 558, 653
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 1 cut(s) 637
SfuI TTCGAA 1 cut(s) 269
SinI GGWCC 2 cut(s) 509, 653
SmlI CTYRAG 2 cut(s) 128, 239
SmoI CTYRAG 2 cut(s) 128, 239
SpeI ACTAGT 1 cut(s) 391
Sse9I AATT 7 cut(s) 271, 441, 452, 532, 568, 584, 640
SsiI CCGC 2 cut(s) 512, 549
SspI AATATT 2 cut(s) 623, 691
SspMI CTAG 2 cut(s) 32, 392
StyD4I CCNGG 1 cut(s) 635
StyI CCWWGG 2 cut(s) 31, 381
TaaI ACNGT 1 cut(s) 411
TaiI ACGT 1 cut(s) 237
TaqI TCGA 3 cut(s) 251, 263, 269
TasI AATT 7 cut(s) 271, 441, 452, 532, 568, 584, 640
TauI GCSGC 1 cut(s) 552
TfiI GAWTC 3 cut(s) 159, 299, 397
TscAI CASTG 1 cut(s) 511
TseFI GTSAC 1 cut(s) 61
Tsp45I GTSAC 1 cut(s) 61
TspDTI ATGAA 1 cut(s) 465
TspRI CASTG 1 cut(s) 511
VpaK11BI GGWCC 2 cut(s) 509, 653
XagI CCTNNNNNAGG 1 cut(s) 30
XapI RAATTY 3 cut(s) 271, 532, 584
XmaJI CCTAGG 1 cut(s) 31
XspI CTAG 2 cut(s) 32, 392
ZraI GACGTC 1 cut(s) 235
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.