Rmu_sc0003177.1_g000027

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0003177.1
OG0020059
Physical Location & Seq
Reverse (-)
102185 .. 105530
3346 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0003177.1_g000027.1.cds

Sequence Viewer

Length: 1248 bp
atgacacacttgtgcctcaacccatccaatcactcgagctcctccccaccgcacactgccgccatcaacacctttatcatgagcagtttagaggacaagcactatgctccgttattcgtcgatttgaacagttgccgctatcttgtagaaattacagaatttccacgcggaatatatggcctcgatgcgagctaccgcagtagattgaaaagaatttcacaattatcaaacattttggaaggtaaaaagtcaaaaatgactcctcggatgaacctgtttagatgccctgcactgtaccacaatctggctcttgacgtggcaggggtgaaaaagaatttaccggatgagtccaggttaacggctctgttgtctctcttcttctcatttcggccatccgagtatcatgtcttgtttcctggaagtattagggcctacgaacggctactcgggatagtggaatctagttggagattgaccgaaattggacttccggagtggttcacctgcctcacgtatatcgataagaaaggacttcaagtatggaactttagaattgattttcctggaaatttcaaatgggagggcaaaggattggctttctgtgctctagaggctttttttacgggatgttgtactataataaaacggctgagtgagagtgggttgccgccaccttccaactgtctggttgagtttgaatttgatatctccaaagacggcgggatgggaagctgcggagtccacgtaaacaaggcaagtgatgatatatacctccagcaccaaacagagttagtctttccagtggacgaattcgccagaccgtacttgacattgaagaagaactgcgacctaaggagagctgagaatttgatgaagcttgatggtcatgatatatatggctcaagacaggaaattgtttcttctagagctctgcatttggttagtgaattggccggcattgacagtggacctcttttgtattctcaggagaaatttgtatctctgagcatattgaagttcatacagacacaatcaccatcacatcatcttgaacttgctaatcaggggaatgaagactccagccttctatctgaacatgacagaatgagtggtttccggatggatgtcactgcagcatctctggttttccaggggaattcggtgctgacatatcatgacggtattctattaaactttccatatggtctaggaaaccaaacatcgagagttaaagaagatgtgggctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

415

Amino Acids

46.7

Weight (kDa)

6.4

Isoelectric Point (pI)

48.3

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020059)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003177.1_g000027 Rmu_sc0007121.1_g000030 Rmu_sc0041970.1_g000001 Rmu_ssc0000432.1_g000121
rosa_roxburghii Rroxscaffold_1G00002560
rosa_samantha Rh5BG548300 Rh5BG549500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 512
Acc36I ACCTGC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 304
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 2 cut(s) 490, 1116
AciI CCGC 8 cut(s) 50, 60, 136, 168, 196, 668, 720, 735
AcoI YGGCCR 2 cut(s) 389, 951
AcsI RAATTY 9 cut(s) 158, 213, 334, 568, 698, 809, 865, 992, 1156
AfaI GTAC 3 cut(s) 296, 634, 824
AfiI CCNNNNNNNGG 2 cut(s) 304, 438
AgsI TTSAA 8 cut(s) 127, 208, 536, 574, 698, 835, 1015, 1052
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 4 cut(s) 350, 415, 562, 1149
AleI CACNNNNGTG 1 cut(s) 10
AluBI AGCT 6 cut(s) 39, 192, 732, 860, 877, 929
AluI AGCT 6 cut(s) 39, 192, 732, 860, 877, 929
Alw21I GWGCWC 3 cut(s) 41, 607, 931
Alw26I GTCTC 1 cut(s) 375
Ama87I CYCGRG 2 cut(s) 34, 446
Aor13HI TCCGGA 2 cut(s) 490, 1116
AoxI GGCC 4 cut(s) 178, 389, 429, 951
ApeKI GCWGC 2 cut(s) 732, 1133
ApoI RAATTY 9 cut(s) 158, 213, 334, 568, 698, 809, 865, 992, 1156
AspS9I GGNCC 2 cut(s) 429, 968
AsuHPI GGTGA 3 cut(s) 337, 493, 1026
AvaI CYCGRG 2 cut(s) 34, 446
AvaII GGWCC 1 cut(s) 968
AxyI CCTNAGG 1 cut(s) 851
BanII GRGCYC 2 cut(s) 41, 931
BbsI GAAGAC 1 cut(s) 1080
Bbv12I GWGCWC 3 cut(s) 41, 607, 931
BbvI GCAGC 2 cut(s) 719, 1145
BccI CCATC 7 cut(s) 31, 71, 400, 718, 875, 1045, 1114
BceAI ACGGC 4 cut(s) 375, 455, 662, 733
BciT130I CCWGG 4 cut(s) 352, 417, 564, 1151
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 4 cut(s) 462, 608, 924, 1208
BfmI CTRYAG 1 cut(s) 1131
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 5 cut(s) 60, 136, 668, 733, 1134
BlsI GCNGC 5 cut(s) 61, 137, 669, 734, 1135
Bme1390I CCNGG 4 cut(s) 352, 417, 564, 1151
Bme18I GGWCC 1 cut(s) 968
BmeT110I CYCGRG 2 cut(s) 34, 446
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 2 cut(s) 429, 968
BmrFI CCNGG 4 cut(s) 352, 417, 564, 1151
BmsI GCATC 3 cut(s) 175, 272, 1145
BpiI GAAGAC 1 cut(s) 1080
BpmI CTGGAG 2 cut(s) 758, 1063
BpuEI CTTGAG 1 cut(s) 886
Bsa29I ATCGAT 1 cut(s) 519
BsaAI YACGTR 2 cut(s) 513, 745
BsaJI CCNNGG 2 cut(s) 263, 1150
BsaWI WCCGGW 3 cut(s) 340, 490, 1116
BsaXI ACNNNNNCTCC 4 cut(s) 23, 53, 729, 759
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 438
Bse118I RCCGGY 1 cut(s) 953
Bse1I ACTGG 1 cut(s) 800
Bse21I CCTNAGG 1 cut(s) 851
BseAI TCCGGA 2 cut(s) 490, 1116
BseBI CCWGG 4 cut(s) 352, 417, 564, 1151
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 2 cut(s) 263, 1150
BseGI GGATG 8 cut(s) 23, 273, 349, 392, 632, 729, 1125, 1129
BseLI CCNNNNNNNGG 2 cut(s) 304, 438
BseMII CTCAG 4 cut(s) 641, 852, 995, 998
BseNI ACTGG 1 cut(s) 800
BseRI GAGGAG 2 cut(s) 31, 252
BseXI GCAGC 2 cut(s) 719, 1145
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 4 cut(s) 180, 391, 431, 953
BshVI ATCGAT 1 cut(s) 519
BsiHKAI GWGCWC 3 cut(s) 41, 607, 931
BsiHKCI CYCGRG 2 cut(s) 34, 446
BsiSI CCGG 4 cut(s) 341, 491, 954, 1117
BslI CCNNNNNNNGG 2 cut(s) 304, 438
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 4 cut(s) 180, 391, 431, 953
BsoBI CYCGRG 2 cut(s) 34, 446
Bsp1286I GDGCHC 3 cut(s) 41, 607, 931
Bsp13I TCCGGA 2 cut(s) 490, 1116
BspACI CCGC 8 cut(s) 50, 60, 136, 168, 196, 668, 720, 735
BspANI GGCC 4 cut(s) 180, 391, 431, 953
BspCNI CTCAG 4 cut(s) 642, 853, 996, 997
BspDI ATCGAT 1 cut(s) 519
BspEI TCCGGA 2 cut(s) 490, 1116
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 3 cut(s) 78, 886, 1174
BspMAI CTGCAG 1 cut(s) 1135
BspMI ACCTGC 1 cut(s) 512
BsrFI RCCGGY 1 cut(s) 953
BsrI ACTGG 1 cut(s) 800
BssAI RCCGGY 1 cut(s) 953
BssECI CCNNGG 2 cut(s) 263, 1150
Bst2UI CCWGG 4 cut(s) 352, 417, 564, 1151
Bst4CI ACNGT 6 cut(s) 131, 294, 683, 822, 965, 1181
Bst6I CTCTTC 1 cut(s) 380
BstBAI YACGTR 2 cut(s) 513, 745
BstC8I GCNNGC 2 cut(s) 190, 955
BstDEI CTNAG 5 cut(s) 650, 851, 861, 984, 1004
BstF5I GGATG 8 cut(s) 23, 273, 349, 392, 632, 729, 1125, 1129
BstFNI CGCG 1 cut(s) 168
BstMAI GTCTC 1 cut(s) 375
BstMWI GCNNNNNNNGC 2 cut(s) 602, 611
BstNI CCWGG 4 cut(s) 352, 417, 564, 1151
BstSCI CCNGG 4 cut(s) 350, 415, 562, 1149
BstSFI CTRYAG 1 cut(s) 1131
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 719, 1145
BstV2I GAAGAC 1 cut(s) 1080
BstXI CCANNNNNNTGG 1 cut(s) 685
Bsu15I ATCGAT 1 cut(s) 519
Bsu36I CCTNAGG 1 cut(s) 851
BsuRI GGCC 4 cut(s) 180, 391, 431, 953
BsuTUI ATCGAT 1 cut(s) 519
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 8 cut(s) 23, 273, 349, 392, 632, 729, 1125, 1129
BtsI GCAGTG 2 cut(s) 54, 1128
BtsIMutI CAGTG 5 cut(s) 54, 290, 807, 970, 1128
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 2 cut(s) 190, 955
CciI TCATGA 3 cut(s) 78, 886, 1174
Cfr10I RCCGGY 1 cut(s) 953
Cfr13I GGNCC 2 cut(s) 429, 968
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 3 cut(s) 295, 633, 823
CviAII CATG 5 cut(s) 79, 404, 887, 1097, 1175
CviQI GTAC 3 cut(s) 295, 633, 823
DdeI CTNAG 5 cut(s) 650, 851, 861, 984, 1004
EaeI YGGCCR 2 cut(s) 389, 951
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Ecl136II GAGCTC 2 cut(s) 39, 929
Eco24I GRGCYC 2 cut(s) 41, 931
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 1 cut(s) 968
Eco53kI GAGCTC 2 cut(s) 39, 929
Eco81I CCTNAGG 1 cut(s) 851
Eco88I CYCGRG 2 cut(s) 34, 446
EcoICRI GAGCTC 2 cut(s) 39, 929
EcoO109I RGGNCCY 1 cut(s) 429
EcoRI GAATTC 2 cut(s) 809, 1156
EcoRII CCWGG 4 cut(s) 350, 415, 562, 1149
EcoRV GATATC 1 cut(s) 706
EcoT38I GRGCYC 2 cut(s) 41, 931
FaeI CATG 5 cut(s) 82, 407, 890, 1100, 1178
FatI CATG 5 cut(s) 78, 403, 886, 1096, 1174
FauI CCCGC 1 cut(s) 713
FauNDI CATATG 1 cut(s) 1201
Fnu4HI GCNGC 5 cut(s) 60, 136, 668, 733, 1134
FokI GGATG 8 cut(s) 10, 280, 356, 379, 639, 736, 1132, 1136
FriOI GRGCYC 2 cut(s) 41, 931
Fsp4HI GCNGC 5 cut(s) 60, 136, 668, 733, 1134
FspBI CTAG 4 cut(s) 462, 608, 924, 1208
GluI GCNGC 5 cut(s) 60, 136, 668, 733, 1134
GsuI CTGGAG 2 cut(s) 758, 1063
HaeIII GGCC 4 cut(s) 180, 391, 431, 953
HapII CCGG 4 cut(s) 341, 491, 954, 1117
Hin1II CATG 5 cut(s) 82, 407, 890, 1100, 1178
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HindIII AAGCTT 1 cut(s) 875
HinfI GANTC 5 cut(s) 259, 347, 458, 738, 1076
HpaI GTTAAC 1 cut(s) 357
HpaII CCGG 4 cut(s) 341, 491, 954, 1117
HphI GGTGA 3 cut(s) 337, 493, 1026
Hpy166II GTNNAC 6 cut(s) 357, 501, 742, 748, 805, 968
Hpy188I TCNGA 4 cut(s) 267, 397, 1005, 1093
Hpy8I GTNNAC 6 cut(s) 357, 501, 742, 748, 805, 968
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 3 cut(s) 233, 684, 1094
HpyCH4III ACNGT 6 cut(s) 131, 294, 683, 822, 965, 1181
HpyCH4IV ACGT 3 cut(s) 315, 512, 744
HpyCH4V TGCA 3 cut(s) 290, 934, 1133
HpyF10VI GCNNNNNNNGC 2 cut(s) 602, 611
HpyF3I CTNAG 5 cut(s) 650, 851, 861, 984, 1004
HpySE526I ACGT 3 cut(s) 315, 512, 744
Hsp92II CATG 5 cut(s) 82, 407, 890, 1100, 1178
Kpn2I TCCGGA 2 cut(s) 490, 1116
KroI GCCGGC 1 cut(s) 953
KroNI GCCGGC 1 cut(s) 955
KspAI GTTAAC 1 cut(s) 357
LmnI GCTCC 2 cut(s) 44, 112
Lsp1109I GCAGC 2 cut(s) 719, 1145
LweI GCATC 3 cut(s) 175, 272, 1145
MaeI CTAG 4 cut(s) 462, 608, 924, 1208
MaeII ACGT 3 cut(s) 315, 512, 744
MaeIII GTNAC 1 cut(s) 1126
MboII GAAGA 7 cut(s) 367, 370, 847, 850, 912, 1085, 1247
MhlI GDGCHC 3 cut(s) 41, 607, 931
MlyI GAGTC 4 cut(s) 253, 356, 747, 1070
MmeI TCCRAC 2 cut(s) 446, 702
MroI TCCGGA 2 cut(s) 490, 1116
MroNI GCCGGC 1 cut(s) 953
MseI TTAA 3 cut(s) 356, 1190, 1230
MslI CAYNNNNRTG 1 cut(s) 10
MspI CCGG 4 cut(s) 341, 491, 954, 1117
MspR9I CCNGG 4 cut(s) 352, 417, 564, 1151
MvaI CCWGG 4 cut(s) 352, 417, 564, 1151
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 2 cut(s) 602, 611
NaeI GCCGGC 1 cut(s) 955
NdeI CATATG 1 cut(s) 1201
NgoMIV GCCGGC 1 cut(s) 953
NlaIII CATG 5 cut(s) 82, 407, 890, 1100, 1178
NmuCI GTSAC 1 cut(s) 1126
OliI CACNNNNGTG 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 34
PagI TCATGA 3 cut(s) 78, 886, 1174
PaqCI CACCTGC 1 cut(s) 512
PdiI GCCGGC 1 cut(s) 955
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 304
PfoI TCCNGGA 2 cut(s) 415, 562
PkrI GCNGC 5 cut(s) 61, 137, 669, 734, 1135
PleI GAGTC 4 cut(s) 253, 355, 746, 1070
PpsI GAGTC 4 cut(s) 253, 355, 746, 1070
Ppu21I YACGTR 2 cut(s) 513, 745
Psp124BI GAGCTC 2 cut(s) 41, 931
Psp6I CCWGG 4 cut(s) 350, 415, 562, 1149
PspGI CCWGG 4 cut(s) 350, 415, 562, 1149
PspPI GGNCC 2 cut(s) 429, 968
PspXI VCTCGAGB 1 cut(s) 34
PstI CTGCAG 1 cut(s) 1135
RsaI GTAC 3 cut(s) 296, 634, 824
RsaNI GTAC 3 cut(s) 295, 633, 823
RseI CAYNNNNRTG 1 cut(s) 10
SacI GAGCTC 2 cut(s) 41, 931
SaqAI TTAA 3 cut(s) 356, 1190, 1230
SatI GCNGC 5 cut(s) 60, 136, 668, 733, 1134
Sau96I GGNCC 2 cut(s) 429, 968
SchI GAGTC 4 cut(s) 253, 356, 747, 1070
ScrFI CCNGG 4 cut(s) 352, 417, 564, 1151
SduI GDGCHC 3 cut(s) 41, 607, 931
SfaNI GCATC 3 cut(s) 175, 272, 1145
SfcI CTRYAG 1 cut(s) 1131
Sfr274I CTCGAG 1 cut(s) 34
SinI GGWCC 1 cut(s) 968
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 2 cut(s) 34, 901
SmoI CTYRAG 2 cut(s) 34, 901
SsiI CCGC 8 cut(s) 50, 60, 136, 168, 196, 668, 720, 735
SspMI CTAG 4 cut(s) 462, 608, 924, 1208
SstI GAGCTC 2 cut(s) 41, 931
StyD4I CCNGG 4 cut(s) 350, 415, 562, 1149
TaaI ACNGT 6 cut(s) 131, 294, 683, 822, 965, 1181
TaiI ACGT 3 cut(s) 318, 515, 747
TaqI TCGA 5 cut(s) 35, 120, 183, 519, 1223
TaqII GACCGA 1 cut(s) 491
TatI WGTACW 1 cut(s) 632
TauI GCSGC 3 cut(s) 62, 138, 670
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 3 cut(s) 356, 1190, 1230
Tru9I TTAA 3 cut(s) 356, 1190, 1230
TscAI CASTG 5 cut(s) 61, 297, 807, 970, 1135
TseFI GTSAC 1 cut(s) 1126
TseI GCWGC 2 cut(s) 732, 1133
Tsp45I GTSAC 1 cut(s) 1126
TspDTI ATGAA 4 cut(s) 284, 887, 1009, 1086
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 5 cut(s) 61, 297, 807, 970, 1135
Van91I CCANNNNNTGG 1 cut(s) 304
VpaK11BI GGWCC 1 cut(s) 968
XapI RAATTY 9 cut(s) 158, 213, 334, 568, 698, 809, 865, 992, 1156
XbaI TCTAGA 2 cut(s) 607, 923
XhoI CTCGAG 1 cut(s) 34
XspI CTAG 4 cut(s) 462, 608, 924, 1208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.