Rmu_sc0007121.1_g000030

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007121.1
OG0020059
Physical Location & Seq
Reverse (-)
118395 .. 118946
552 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0007121.1_g000030.1.cds

Sequence Viewer

Length: 552 bp
atgacacacttgtgcctcaacccatccaatcactcgagctcctccccaccgcacactgccgccatcaacacctttatcatgagcagtttagaggacaagcactatgctccgttattcgtcgatttgaacagttgccgctatcttgtagaaattacagaatttccacgcggaatatatggcctcgatgcgagctaccgcagtagattgaaaagaatttcacaattatcaaacattttggaaggtaaaaagtcaaaaatgactcctcggatgaacctgtttagatgccctgcactgtaccacaatctggctcttgacgtggcaggggtgaaaaagaatttaccggatgagtccaggttaacggctctgttgtctctcttcttctcatttcggccatccgagtatcatgtcttgtttcctggaagtattagggcctacgaacggctactcgggatagtggaatctagttggagattgaccgaaattggacttccggagtggttcacctgcctcacgtatatcgataagaaaggacttccaagtctgcaactttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

183

Amino Acids

20.85

Weight (kDa)

9.08

Isoelectric Point (pI)

57.54

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020059)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003177.1_g000027 Rmu_sc0007121.1_g000030 Rmu_sc0041970.1_g000001 Rmu_ssc0000432.1_g000121
rosa_roxburghii Rroxscaffold_1G00002560
rosa_samantha Rh5BG548300 Rh5BG549500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 512
AasI GACNNNNNNGTC 1 cut(s) 537
Acc36I ACCTGC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 304
AccII CGCG 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 490
AciI CCGC 5 cut(s) 50, 60, 136, 168, 196
AcoI YGGCCR 1 cut(s) 389
AcsI RAATTY 3 cut(s) 158, 213, 334
AfaI GTAC 1 cut(s) 296
AfiI CCNNNNNNNGG 2 cut(s) 304, 438
AgsI TTSAA 2 cut(s) 127, 208
AjiI CACGTC 1 cut(s) 316
AjnI CCWGG 2 cut(s) 350, 415
AleI CACNNNNGTG 1 cut(s) 10
AluBI AGCT 2 cut(s) 39, 192
AluI AGCT 2 cut(s) 39, 192
Alw21I GWGCWC 1 cut(s) 41
Alw26I GTCTC 1 cut(s) 375
Ama87I CYCGRG 2 cut(s) 34, 446
Aor13HI TCCGGA 1 cut(s) 490
AoxI GGCC 3 cut(s) 178, 389, 429
ApoI RAATTY 3 cut(s) 158, 213, 334
AspS9I GGNCC 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 337, 493
AvaI CYCGRG 2 cut(s) 34, 446
BanII GRGCYC 1 cut(s) 41
Bbv12I GWGCWC 1 cut(s) 41
BccI CCATC 3 cut(s) 31, 71, 400
BceAI ACGGC 2 cut(s) 375, 455
BciT130I CCWGG 2 cut(s) 352, 417
BcoDI GTCTC 1 cut(s) 375
BfaI CTAG 1 cut(s) 462
BfuAI ACCTGC 1 cut(s) 512
BisI GCNGC 2 cut(s) 60, 136
BlsI GCNGC 2 cut(s) 61, 137
Bme1390I CCNGG 2 cut(s) 352, 417
BmeT110I CYCGRG 2 cut(s) 34, 446
BmgBI CACGTC 1 cut(s) 316
BmgT120I GGNCC 1 cut(s) 429
BmrFI CCNGG 2 cut(s) 352, 417
BmsI GCATC 2 cut(s) 175, 272
Bsa29I ATCGAT 1 cut(s) 519
BsaAI YACGTR 1 cut(s) 513
BsaJI CCNNGG 1 cut(s) 263
BsaWI WCCGGW 2 cut(s) 340, 490
BsaXI ACNNNNNCTCC 2 cut(s) 23, 53
Bsc4I CCNNNNNNNGG 2 cut(s) 304, 438
BseAI TCCGGA 1 cut(s) 490
BseBI CCWGG 2 cut(s) 352, 417
BseCI ATCGAT 1 cut(s) 519
BseDI CCNNGG 1 cut(s) 263
BseGI GGATG 4 cut(s) 23, 273, 349, 392
BseLI CCNNNNNNNGG 2 cut(s) 304, 438
BseRI GAGGAG 2 cut(s) 31, 252
BsgI GTGCAG 1 cut(s) 273
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 3 cut(s) 180, 391, 431
BshVI ATCGAT 1 cut(s) 519
BsiHKAI GWGCWC 1 cut(s) 41
BsiHKCI CYCGRG 2 cut(s) 34, 446
BsiSI CCGG 2 cut(s) 341, 491
BslI CCNNNNNNNGG 2 cut(s) 304, 438
BsmAI GTCTC 1 cut(s) 375
BsnI GGCC 3 cut(s) 180, 391, 431
BsoBI CYCGRG 2 cut(s) 34, 446
Bsp1286I GDGCHC 1 cut(s) 41
Bsp13I TCCGGA 1 cut(s) 490
BspACI CCGC 5 cut(s) 50, 60, 136, 168, 196
BspANI GGCC 3 cut(s) 180, 391, 431
BspDI ATCGAT 1 cut(s) 519
BspEI TCCGGA 1 cut(s) 490
BspFNI CGCG 1 cut(s) 168
BspHI TCATGA 1 cut(s) 78
BspMI ACCTGC 1 cut(s) 512
BssECI CCNNGG 1 cut(s) 263
Bst2UI CCWGG 2 cut(s) 352, 417
Bst4CI ACNGT 2 cut(s) 131, 294
Bst6I CTCTTC 1 cut(s) 380
BstBAI YACGTR 1 cut(s) 513
BstC8I GCNNGC 1 cut(s) 190
BstF5I GGATG 4 cut(s) 23, 273, 349, 392
BstFNI CGCG 1 cut(s) 168
BstMAI GTCTC 1 cut(s) 375
BstNI CCWGG 2 cut(s) 352, 417
BstSCI CCNGG 2 cut(s) 350, 415
BstUI CGCG 1 cut(s) 168
Bsu15I ATCGAT 1 cut(s) 519
BsuRI GGCC 3 cut(s) 180, 391, 431
BsuTUI ATCGAT 1 cut(s) 519
BtrI CACGTC 1 cut(s) 316
BtsCI GGATG 4 cut(s) 23, 273, 349, 392
BtsI GCAGTG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 54, 290
BveI ACCTGC 1 cut(s) 512
Cac8I GCNNGC 1 cut(s) 190
CciI TCATGA 1 cut(s) 78
Cfr13I GGNCC 1 cut(s) 429
ClaI ATCGAT 1 cut(s) 519
Csp6I GTAC 1 cut(s) 295
CviAII CATG 2 cut(s) 79, 404
CviJI RGCY 8 cut(s) 39, 180, 192, 308, 362, 391, 431, 442
CviKI_1 RGCY 8 cut(s) 39, 180, 192, 308, 362, 391, 431, 442
CviQI GTAC 1 cut(s) 295
DrdI GACNNNNNNGTC 1 cut(s) 537
DseDI GACNNNNNNGTC 1 cut(s) 537
EaeI YGGCCR 1 cut(s) 389
Eam1104I CTCTTC 1 cut(s) 380
EarI CTCTTC 1 cut(s) 380
Ecl136II GAGCTC 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 41
Eco53kI GAGCTC 1 cut(s) 39
Eco88I CYCGRG 2 cut(s) 34, 446
EcoICRI GAGCTC 1 cut(s) 39
EcoO109I RGGNCCY 1 cut(s) 429
EcoRII CCWGG 2 cut(s) 350, 415
EcoT38I GRGCYC 1 cut(s) 41
FaeI CATG 2 cut(s) 82, 407
FaiI YATR 6 cut(s) 80, 105, 175, 177, 405, 516
FatI CATG 2 cut(s) 78, 403
Fnu4HI GCNGC 2 cut(s) 60, 136
FokI GGATG 4 cut(s) 10, 280, 356, 379
FriOI GRGCYC 1 cut(s) 41
Fsp4HI GCNGC 2 cut(s) 60, 136
FspBI CTAG 1 cut(s) 462
GluI GCNGC 2 cut(s) 60, 136
HaeIII GGCC 3 cut(s) 180, 391, 431
HapII CCGG 2 cut(s) 341, 491
Hin1II CATG 2 cut(s) 82, 407
HincII GTYRAC 1 cut(s) 357
HindII GTYRAC 1 cut(s) 357
HinfI GANTC 3 cut(s) 259, 347, 458
HpaI GTTAAC 1 cut(s) 357
HpaII CCGG 2 cut(s) 341, 491
HphI GGTGA 2 cut(s) 337, 493
Hpy166II GTNNAC 2 cut(s) 357, 501
Hpy188I TCNGA 2 cut(s) 267, 397
Hpy188III TCNNGA 4 cut(s) 79, 311, 448, 491
Hpy8I GTNNAC 2 cut(s) 357, 501
Hpy99I CGWCG 1 cut(s) 122
HpyAV CCTTC 1 cut(s) 233
HpyCH4III ACNGT 2 cut(s) 131, 294
HpyCH4IV ACGT 2 cut(s) 315, 512
HpyCH4V TGCA 2 cut(s) 290, 544
HpySE526I ACGT 2 cut(s) 315, 512
Hsp92II CATG 2 cut(s) 82, 407
Kpn2I TCCGGA 1 cut(s) 490
KspAI GTTAAC 1 cut(s) 357
LmnI GCTCC 2 cut(s) 44, 112
LweI GCATC 2 cut(s) 175, 272
MaeI CTAG 1 cut(s) 462
MaeII ACGT 2 cut(s) 315, 512
MboII GAAGA 2 cut(s) 367, 370
MhlI GDGCHC 1 cut(s) 41
MluCI AATT 6 cut(s) 150, 158, 213, 221, 334, 480
MlyI GAGTC 2 cut(s) 253, 356
MmeI TCCRAC 1 cut(s) 446
MnlI CCTC 6 cut(s) 26, 52, 85, 191, 273, 518
MroI TCCGGA 1 cut(s) 490
MseI TTAA 1 cut(s) 356
MslI CAYNNNNRTG 1 cut(s) 10
MspI CCGG 2 cut(s) 341, 491
MspR9I CCNGG 2 cut(s) 352, 417
MvaI CCWGG 2 cut(s) 352, 417
MvnI CGCG 1 cut(s) 168
NlaIII CATG 2 cut(s) 82, 407
OliI CACNNNNGTG 1 cut(s) 10
PaeR7I CTCGAG 1 cut(s) 34
PagI TCATGA 1 cut(s) 78
PaqCI CACCTGC 1 cut(s) 512
PfeI GAWTC 1 cut(s) 458
PflMI CCANNNNNTGG 1 cut(s) 304
PfoI TCCNGGA 1 cut(s) 415
PkrI GCNGC 2 cut(s) 61, 137
PleI GAGTC 2 cut(s) 253, 355
PpsI GAGTC 2 cut(s) 253, 355
Ppu21I YACGTR 1 cut(s) 513
Psp124BI GAGCTC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 350, 415
PspGI CCWGG 2 cut(s) 350, 415
PspPI GGNCC 1 cut(s) 429
PspXI VCTCGAGB 1 cut(s) 34
RsaI GTAC 1 cut(s) 296
RsaNI GTAC 1 cut(s) 295
RseI CAYNNNNRTG 1 cut(s) 10
SacI GAGCTC 1 cut(s) 41
SaqAI TTAA 1 cut(s) 356
SatI GCNGC 2 cut(s) 60, 136
Sau96I GGNCC 1 cut(s) 429
SchI GAGTC 2 cut(s) 253, 356
ScrFI CCNGG 2 cut(s) 352, 417
SduI GDGCHC 1 cut(s) 41
SetI ASST 9 cut(s) 41, 74, 194, 244, 276, 318, 356, 506, 515
SfaNI GCATC 2 cut(s) 175, 272
Sfr274I CTCGAG 1 cut(s) 34
SlaI CTCGAG 1 cut(s) 34
SmiMI CAYNNNNRTG 1 cut(s) 10
SmlI CTYRAG 1 cut(s) 34
SmoI CTYRAG 1 cut(s) 34
Sse9I AATT 6 cut(s) 150, 158, 213, 221, 334, 480
SsiI CCGC 5 cut(s) 50, 60, 136, 168, 196
SspMI CTAG 1 cut(s) 462
SstI GAGCTC 1 cut(s) 41
StyD4I CCNGG 2 cut(s) 350, 415
TaaI ACNGT 2 cut(s) 131, 294
TaiI ACGT 2 cut(s) 318, 515
TaqI TCGA 4 cut(s) 35, 120, 183, 519
TaqII GACCGA 1 cut(s) 491
TasI AATT 6 cut(s) 150, 158, 213, 221, 334, 480
TauI GCSGC 2 cut(s) 62, 138
TfiI GAWTC 1 cut(s) 458
Tru1I TTAA 1 cut(s) 356
Tru9I TTAA 1 cut(s) 356
TscAI CASTG 2 cut(s) 61, 297
TspDTI ATGAA 1 cut(s) 284
TspGWI ACGGA 1 cut(s) 99
TspRI CASTG 2 cut(s) 61, 297
Van91I CCANNNNNTGG 1 cut(s) 304
XapI RAATTY 3 cut(s) 158, 213, 334
XhoI CTCGAG 1 cut(s) 34
XspI CTAG 1 cut(s) 462
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.