Rmu_ssc0000432.1_g000121

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_ssc0000432.1
OG0020059
Physical Location & Seq
Forward (+)
420569 .. 422080
1512 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_ssc0000432.1_g000121.1.cds

Sequence Viewer

Length: 1512 bp
atgcaaaatttgaaatcactcaatttgggaaattgcgatgctgtaacaactttgtccgacttctctaaattcccaaacttagaggagttaaaaataagaggtagcagaaaagttgatgagtttgaaattgtggaagagatgaaatccttaaaaaggctggatctaagcggtactgcaatcaaagaattaccttcatcagcaatcagatatctcattaatctggaacaattaactttatcaggttgcaagaatcttaaacatgtgccatgcaacatttttgagttgcaacatctacagcttcttgatctctcgggttgtgaaaagctggttacatttccaacaaagtcagagatgaaatccttaaaaaggctggatctaagcggtactgcaatcaaagaattaccttcatcagcaatcagatatctcattaatctggaacaattaactttatcaggttgcaagaaacttgaacatgtgccgtgcaacatttttgagttacaacatttacggcttcttgatctctcgggttgtgaaaagctggttacatttccaacaaagtcaaagttttcaactgaatcgaccagtttagaggacaaggactatgctccgttattcgtcaatttgagcagttgctacgatcttgcagaaattgtagaatttccgcgagaaatatatgacgtaaatgcgagctgctgctctgtgttgaaaagaatttcaaaattgtcaaacattttggaaggtaaagaatcaaaaatgactccctggatgaatttgtttcattgcggtaaactgtaccgcaccctggctcgtgatgtagcaaaggtgaaaaagaatttaccggataattccaggttaacggctctgttgtctctcttcttctcatgtcggcaatctgagtatcaggtcttgtttcctggaagtattaggagctacgaacggctattcgggaaagtggaatctagttggagattgcccgaaattggacttccacagtggttcacctgccgcatgtatatcagtaacaaaggacttcaagtatgcaactttgaaattgattttcctggaaatttcaaatgggaggacaaaggattggctttctctgctttaactattgataatgaaaatttgtatacgaaagcatatatgagagtactcaaaatctacattaatgatatacgcatcattgacgcatccgaaaagccggaagacagtaggtcgggatcgttctatgatttagtatggttgtactatgtgccattcgatactataataaaacggctgggtgagagtgggttaccgccgccttccatgtgtctggttaagttcgaatttgaatataaaactctcgatggtggtgaggacattaacggctggattggaagctgcggggtccacgtagtaatgccagaggatgaaggcccagttgtaaatgggattgtacgtccatcaactttgataatggggactaactcctacccgggggacgatgctgaagatccaccaactgtttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

503

Amino Acids

57.03

Weight (kDa)

5.76

Isoelectric Point (pI)

44.51

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020059)

Species Orthologous Gene IDs
rosa_multiflora Rmu_sc0003177.1_g000027 Rmu_sc0007121.1_g000030 Rmu_sc0041970.1_g000001 Rmu_ssc0000432.1_g000121
rosa_roxburghii Rroxscaffold_1G00002560
rosa_samantha Rh5BG548300 Rh5BG549500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1010
Acc36I ACCTGC 1 cut(s) 1010
AccI GTMKAC 1 cut(s) 1130
AccII CGCG 1 cut(s) 664
AciI CCGC 9 cut(s) 168, 381, 662, 783, 796, 1006, 1298, 1301, 1386
AclWI GGATC 4 cut(s) 168, 381, 1228, 1490
AcsI RAATTY 9 cut(s) 7, 68, 656, 711, 769, 832, 1066, 1123, 1328
AcuI CTGAAG 1 cut(s) 1512
AfaI GTAC 6 cut(s) 172, 385, 794, 1152, 1247, 1440
AfiI CCNNNNNNNGG 5 cut(s) 153, 366, 802, 980, 1479
AflIII ACRYGT 2 cut(s) 259, 472
AhdI GACNNNNNGTC 1 cut(s) 1213
AjnI CCWGG 5 cut(s) 761, 801, 848, 913, 1060
AluBI AGCT 6 cut(s) 298, 325, 538, 690, 930, 1383
AluI AGCT 6 cut(s) 298, 325, 538, 690, 930, 1383
Alw26I GTCTC 1 cut(s) 873
AlwI GGATC 4 cut(s) 168, 381, 1228, 1490
Ama87I CYCGRG 3 cut(s) 310, 523, 1477
AoxI GGCC 1 cut(s) 1417
ApeKI GCWGC 3 cut(s) 690, 693, 1383
ApoI RAATTY 9 cut(s) 7, 68, 656, 711, 769, 832, 1066, 1123, 1328
AseI ATTAAT 3 cut(s) 216, 429, 1167
AspS9I GGNCC 2 cut(s) 1390, 1418
AsuC2I CCSGG 2 cut(s) 1478, 1479
AsuHPI GGTGA 4 cut(s) 835, 991, 1295, 1367
AsuII TTCGAA 1 cut(s) 1326
AvaI CYCGRG 3 cut(s) 310, 523, 1477
AvaII GGWCC 1 cut(s) 1390
BauI CACGAG 1 cut(s) 807
BbsI GAAGAC 1 cut(s) 1212
BbvI GCAGC 3 cut(s) 677, 680, 1370
BccI CCATC 2 cut(s) 1343, 1453
BceAI ACGGC 6 cut(s) 463, 524, 873, 953, 1292, 1384
BciT130I CCWGG 5 cut(s) 763, 803, 850, 915, 1062
BcnI CCSGG 2 cut(s) 1478, 1479
BcoDI GTCTC 1 cut(s) 873
BfaI CTAG 1 cut(s) 960
BfmI CTRYAG 1 cut(s) 293
BfuAI ACCTGC 1 cut(s) 1010
BisI GCNGC 5 cut(s) 691, 694, 1006, 1301, 1384
BlsI GCNGC 5 cut(s) 692, 695, 1007, 1302, 1385
BmcAI AGTACT 1 cut(s) 1152
Bme1390I CCNGG 7 cut(s) 763, 803, 850, 915, 1062, 1478, 1479
Bme18I GGWCC 1 cut(s) 1390
BmeRI GACNNNNNGTC 1 cut(s) 1213
BmeT110I CYCGRG 3 cut(s) 310, 523, 1477
BmgT120I GGNCC 2 cut(s) 1390, 1418
BmiI GGNNCC 1 cut(s) 1391
BmrFI CCNGG 7 cut(s) 763, 803, 850, 915, 1062, 1478, 1479
BmrI ACTGGG 1 cut(s) 1415
BmsI GCATC 4 cut(s) 28, 1188, 1199, 1477
BmuI ACTGGG 1 cut(s) 1415
BpiI GAAGAC 1 cut(s) 1212
Bpu14I TTCGAA 1 cut(s) 1326
BpuMI CCSGG 2 cut(s) 1478, 1479
BsaAI YACGTR 1 cut(s) 1396
BsaJI CCNNGG 4 cut(s) 761, 801, 1477, 1478
BsaWI WCCGGW 1 cut(s) 838
Bsc4I CCNNNNNNNGG 5 cut(s) 153, 366, 802, 980, 1479
Bse1I ACTGG 2 cut(s) 582, 1421
Bse3DI GCAATG 1 cut(s) 778
BseBI CCWGG 5 cut(s) 763, 803, 850, 915, 1062
BseDI CCNNGG 4 cut(s) 761, 801, 1477, 1478
BseGI GGATG 3 cut(s) 771, 1190, 1417
BseLI CCNNNNNNNGG 5 cut(s) 153, 366, 802, 980, 1479
BseMI GCAATG 1 cut(s) 778
BseMII CTCAG 1 cut(s) 885
BseNI ACTGG 2 cut(s) 582, 1421
BseRI GAGGAG 1 cut(s) 98
BseXI GCAGC 3 cut(s) 677, 680, 1370
BseYI CCCAGC 1 cut(s) 1279
Bsh1236I CGCG 1 cut(s) 664
BshFI GGCC 1 cut(s) 1419
BsiHKCI CYCGRG 3 cut(s) 310, 523, 1477
BsiSI CCGG 3 cut(s) 839, 1202, 1478
BslFI GGGAC 2 cut(s) 1477, 1496
BslI CCNNNNNNNGG 5 cut(s) 153, 366, 802, 980, 1479
BsmAI GTCTC 1 cut(s) 873
BsmFI GGGAC 2 cut(s) 1477, 1496
BsnI GGCC 1 cut(s) 1419
BsoBI CYCGRG 3 cut(s) 310, 523, 1477
Bsp119I TTCGAA 1 cut(s) 1326
Bsp143I GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
BspACI CCGC 9 cut(s) 168, 381, 662, 783, 796, 1006, 1298, 1301, 1386
BspANI GGCC 1 cut(s) 1419
BspCNI CTCAG 1 cut(s) 886
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 1391
BspMI ACCTGC 1 cut(s) 1010
BspPI GGATC 4 cut(s) 168, 381, 1228, 1490
BspT104I TTCGAA 1 cut(s) 1326
BsrDI GCAATG 1 cut(s) 778
BsrI ACTGG 2 cut(s) 582, 1421
BssECI CCNNGG 4 cut(s) 761, 801, 1477, 1478
BssMI GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
BssNAI GTATAC 1 cut(s) 1131
BssSI CACGAG 1 cut(s) 807
Bst1107I GTATAC 1 cut(s) 1131
Bst2BI CACGAG 1 cut(s) 807
Bst2UI CCWGG 5 cut(s) 763, 803, 850, 915, 1062
Bst4CI ACNGT 4 cut(s) 792, 993, 1211, 1507
Bst6I CTCTTC 2 cut(s) 129, 878
BstBAI YACGTR 1 cut(s) 1396
BstBI TTCGAA 1 cut(s) 1326
BstC8I GCNNGC 1 cut(s) 688
BstDEI CTNAG 4 cut(s) 79, 164, 377, 894
BstEII GGTNACC 1 cut(s) 1293
BstENI CCTNNNNNAGG 2 cut(s) 151, 364
BstF5I GGATG 3 cut(s) 771, 1190, 1417
BstFNI CGCG 1 cut(s) 664
BstKTI GATC 7 cut(s) 163, 307, 376, 520, 640, 1223, 1498
BstMAI GTCTC 1 cut(s) 873
BstMBI GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
BstMWI GCNNNNNNNGC 1 cut(s) 1100
BstNI CCWGG 5 cut(s) 763, 803, 850, 915, 1062
BstNSI RCATGY 3 cut(s) 263, 476, 1012
BstPI GGTNACC 1 cut(s) 1293
BstSCI CCNGG 7 cut(s) 761, 801, 848, 913, 1060, 1476, 1477
BstSFI CTRYAG 1 cut(s) 293
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 3 cut(s) 677, 680, 1370
BstV2I GAAGAC 1 cut(s) 1212
BstX2I RGATCY 3 cut(s) 160, 373, 1495
BstXI CCANNNNNNTGG 1 cut(s) 1315
BstYI RGATCY 3 cut(s) 160, 373, 1495
BstZ17I GTATAC 1 cut(s) 1131
BsuRI GGCC 1 cut(s) 1419
BtgZI GCGATG 1 cut(s) 51
BtsCI GGATG 3 cut(s) 771, 1190, 1417
BtsIMutI CAGTG 1 cut(s) 998
BveI ACCTGC 1 cut(s) 1010
Cac8I GCNNGC 1 cut(s) 688
Cfr13I GGNCC 2 cut(s) 1390, 1418
Cfr9I CCCGGG 1 cut(s) 1477
CseI GACGC 1 cut(s) 1196
Csp6I GTAC 6 cut(s) 171, 384, 793, 1151, 1246, 1439
CviAII CATG 6 cut(s) 260, 267, 473, 882, 1009, 1309
CviQI GTAC 6 cut(s) 171, 384, 793, 1151, 1246, 1439
DdeI CTNAG 4 cut(s) 79, 164, 377, 894
DpnI GATC 7 cut(s) 162, 306, 375, 519, 639, 1222, 1497
DpnII GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
DriI GACNNNNNGTC 1 cut(s) 1213
Eam1104I CTCTTC 2 cut(s) 129, 878
Eam1105I GACNNNNNGTC 1 cut(s) 1213
EarI CTCTTC 2 cut(s) 129, 878
Eco32I GATATC 2 cut(s) 209, 422
Eco47I GGWCC 1 cut(s) 1390
Eco57I CTGAAG 1 cut(s) 1512
Eco88I CYCGRG 3 cut(s) 310, 523, 1477
Eco91I GGTNACC 1 cut(s) 1293
EcoNI CCTNNNNNAGG 2 cut(s) 151, 364
EcoO65I GGTNACC 1 cut(s) 1293
EcoRII CCWGG 5 cut(s) 761, 801, 848, 913, 1060
EcoRV GATATC 2 cut(s) 209, 422
FaeI CATG 6 cut(s) 263, 270, 476, 885, 1012, 1312
FaqI GGGAC 2 cut(s) 1477, 1496
FatI CATG 6 cut(s) 259, 266, 472, 881, 1008, 1308
FauI CCCGC 1 cut(s) 1379
FblI GTMKAC 1 cut(s) 1130
Fnu4HI GCNGC 5 cut(s) 691, 694, 1006, 1301, 1384
FokI GGATG 3 cut(s) 778, 1177, 1424
Fsp4HI GCNGC 5 cut(s) 691, 694, 1006, 1301, 1384
FspBI CTAG 1 cut(s) 960
GluI GCNGC 5 cut(s) 691, 694, 1006, 1301, 1384
GsaI CCCAGC 1 cut(s) 1283
HaeIII GGCC 1 cut(s) 1419
HapII CCGG 3 cut(s) 839, 1202, 1478
HgaI GACGC 1 cut(s) 1196
Hin1II CATG 6 cut(s) 263, 270, 476, 885, 1012, 1312
HincII GTYRAC 1 cut(s) 855
HindII GTYRAC 1 cut(s) 855
HinfI GANTC 5 cut(s) 250, 575, 746, 757, 956
HpaI GTTAAC 1 cut(s) 855
HpaII CCGG 3 cut(s) 839, 1202, 1478
HphI GGTGA 4 cut(s) 835, 991, 1295, 1367
Hpy166II GTNNAC 5 cut(s) 788, 855, 999, 1131, 1393
Hpy188I TCNGA 6 cut(s) 58, 206, 349, 419, 895, 1195
Hpy188III TCNNGA 8 cut(s) 221, 302, 434, 515, 809, 946, 1218, 1346
Hpy8I GTNNAC 5 cut(s) 788, 855, 999, 1131, 1393
HpyAV CCTTC 5 cut(s) 201, 414, 731, 1314, 1409
HpyCH4III ACNGT 4 cut(s) 792, 993, 1211, 1507
HpyCH4IV ACGT 3 cut(s) 678, 1395, 1441
HpyF10VI GCNNNNNNNGC 1 cut(s) 1100
HpyF3I CTNAG 4 cut(s) 79, 164, 377, 894
HpySE526I ACGT 3 cut(s) 678, 1395, 1441
Hsp92II CATG 6 cut(s) 263, 270, 476, 885, 1012, 1312
KspAI GTTAAC 1 cut(s) 855
Kzo9I GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
LmnI GCTCC 2 cut(s) 610, 927
Lsp1109I GCAGC 3 cut(s) 677, 680, 1370
LweI GCATC 4 cut(s) 28, 1188, 1199, 1477
MaeI CTAG 1 cut(s) 960
MaeII ACGT 3 cut(s) 678, 1395, 1441
MaeIII GTNAC 6 cut(s) 43, 328, 495, 541, 1019, 1293
MalI GATC 7 cut(s) 162, 306, 375, 519, 639, 1222, 1497
MboI GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
MboII GAAGA 5 cut(s) 146, 865, 868, 1217, 1505
MflI RGATCY 3 cut(s) 160, 373, 1495
MlyI GAGTC 1 cut(s) 751
MmeI TCCRAC 4 cut(s) 81, 362, 575, 944
MnlI CCTC 6 cut(s) 76, 92, 583, 1072, 1351, 1402
MspI CCGG 3 cut(s) 839, 1202, 1478
MspR9I CCNGG 7 cut(s) 763, 803, 850, 915, 1062, 1478, 1479
MvaI CCWGG 5 cut(s) 763, 803, 850, 915, 1062
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 1 cut(s) 1100
NciI CCSGG 2 cut(s) 1478, 1479
NdeII GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
NlaIII CATG 6 cut(s) 263, 270, 476, 885, 1012, 1312
NlaIV GGNNCC 1 cut(s) 1391
NspI RCATGY 3 cut(s) 263, 476, 1012
NspV TTCGAA 1 cut(s) 1326
PaqCI CACCTGC 1 cut(s) 1010
PciI ACATGT 2 cut(s) 259, 472
PfeI GAWTC 4 cut(s) 250, 575, 746, 956
PfoI TCCNGGA 2 cut(s) 913, 1060
PkrI GCNGC 5 cut(s) 692, 695, 1007, 1302, 1385
PleI GAGTC 1 cut(s) 751
PpsI GAGTC 1 cut(s) 751
Ppu21I YACGTR 1 cut(s) 1396
PscI ACATGT 2 cut(s) 259, 472
PshBI ATTAAT 3 cut(s) 216, 429, 1167
Psp6I CCWGG 5 cut(s) 761, 801, 848, 913, 1060
PspEI GGTNACC 1 cut(s) 1293
PspFI CCCAGC 1 cut(s) 1279
PspGI CCWGG 5 cut(s) 761, 801, 848, 913, 1060
PspN4I GGNNCC 1 cut(s) 1391
PspPI GGNCC 2 cut(s) 1390, 1418
PsuI RGATCY 3 cut(s) 160, 373, 1495
RsaI GTAC 6 cut(s) 172, 385, 794, 1152, 1247, 1440
RsaNI GTAC 6 cut(s) 171, 384, 793, 1151, 1246, 1439
SatI GCNGC 5 cut(s) 691, 694, 1006, 1301, 1384
Sau3AI GATC 7 cut(s) 160, 304, 373, 517, 637, 1220, 1495
Sau96I GGNCC 2 cut(s) 1390, 1418
ScaI AGTACT 1 cut(s) 1152
SchI GAGTC 1 cut(s) 751
ScrFI CCNGG 7 cut(s) 763, 803, 850, 915, 1062, 1478, 1479
SfaNI GCATC 4 cut(s) 28, 1188, 1199, 1477
SfcI CTRYAG 1 cut(s) 293
SfuI TTCGAA 1 cut(s) 1326
SinI GGWCC 1 cut(s) 1390
SmaI CCCGGG 1 cut(s) 1479
SsiI CCGC 9 cut(s) 168, 381, 662, 783, 796, 1006, 1298, 1301, 1386
SspMI CTAG 1 cut(s) 960
StyD4I CCNGG 7 cut(s) 761, 801, 848, 913, 1060, 1476, 1477
TaaI ACNGT 4 cut(s) 792, 993, 1211, 1507
TaiI ACGT 3 cut(s) 681, 1398, 1444
TaqI TCGA 4 cut(s) 578, 1260, 1326, 1347
TatI WGTACW 2 cut(s) 1150, 1245
TauI GCSGC 2 cut(s) 1008, 1303
TfiI GAWTC 4 cut(s) 250, 575, 746, 956
TscAI CASTG 1 cut(s) 998
TseI GCWGC 3 cut(s) 690, 693, 1383
TspDTI ATGAA 8 cut(s) 155, 183, 368, 396, 767, 782, 1134, 1428
TspGWI ACGGA 1 cut(s) 597
TspMI CCCGGG 1 cut(s) 1477
TspRI CASTG 1 cut(s) 998
VpaK11BI GGWCC 1 cut(s) 1390
VspI ATTAAT 3 cut(s) 216, 429, 1167
XagI CCTNNNNNAGG 2 cut(s) 151, 364
XapI RAATTY 9 cut(s) 7, 68, 656, 711, 769, 832, 1066, 1123, 1328
XceI RCATGY 3 cut(s) 263, 476, 1012
XmaI CCCGGG 1 cut(s) 1477
XmiI GTMKAC 1 cut(s) 1130
XspI CTAG 1 cut(s) 960
ZrmI AGTACT 1 cut(s) 1152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.