Protein of unknown function (DUF627)
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT3G47890 AT3G47890 AT3G47910 AT3G47910 |
| fragaria_vesca | FvH4_5g15740 FvH4_5g15740 |
| malus_domestica | MD06G1185800.v1.1 MD14G1192000.v1.1 |
| prunus_persica | Prupe.5G184600_v2.0.a1 Prupe.5G184600_v2.0.a1 |
| pyrus_communis | pycom06g16520 pycom14g15970 |
| rosa_chinensis | RchiOBHm_Chr7g0180371 |
| rosa_laevigata | RLG00000005273 |
| rosa_multiflora | Rmu_sc0003526.1_g000010 |
| rosa_roxburghii | Rroxscaffold_3G00272950 |
| rosa_rugosa | Rorug06G0432500 |
| rosa_samantha | Rh7AG033100 Rh7BG033000 Rh7CG034900 Rh7DG033500 |
| rosa_wichuraiana | Rw7G002760 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 1676, 2283 |
| Acc36I | ACCTGC | 3 cut(s) | 180, 1676, 2283 |
| AccB1I | GGYRCC | 1 cut(s) | 952 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1861 |
| AccI | GTMKAC | 1 cut(s) | 1637 |
| AccII | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| AclI | AACGTT | 1 cut(s) | 2496 |
| AclWI | GGATC | 9 cut(s) | 617, 1941, 3366, 3379, 3672, 4067, 4080, 4730, 4743 |
| AcoI | YGGCCR | 5 cut(s) | 549, 688, 851, 1694, 3545 |
| AcuI | CTGAAG | 2 cut(s) | 2795, 4257 |
| AcvI | CACGTG | 1 cut(s) | 694 |
| AcyI | GRCGYC | 3 cut(s) | 129, 416, 881 |
| AdeI | CACNNNGTG | 1 cut(s) | 402 |
| AfaI | GTAC | 4 cut(s) | 2986, 3789, 3943, 4747 |
| AflII | CTTAAG | 2 cut(s) | 1160, 3332 |
| AflIII | ACRYGT | 2 cut(s) | 4618, 4652 |
| AgeI | ACCGGT | 1 cut(s) | 4269 |
| AhdI | GACNNNNNGTC | 2 cut(s) | 133, 1660 |
| AjiI | CACGTC | 1 cut(s) | 750 |
| AjnI | CCWGG | 9 cut(s) | 926, 1890, 2156, 2198, 3490, 3547, 3765, 3936, 4209 |
| AjuI | GAANNNNNNNTTGG | 4 cut(s) | 2384, 2416, 4529, 4561 |
| AleI | CACNNNNGTG | 2 cut(s) | 1876, 3584 |
| Alw21I | GWGCWC | 6 cut(s) | 495, 708, 3006, 3475, 4368, 4474 |
| Alw26I | GTCTC | 7 cut(s) | 732, 1432, 2629, 2740, 2908, 3181, 3721 |
| Alw44I | GTGCAC | 2 cut(s) | 3471, 4364 |
| AlwI | GGATC | 9 cut(s) | 617, 1941, 3366, 3379, 3672, 4067, 4080, 4730, 4743 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 1936, 3071 |
| Ama87I | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| ApaLI | GTGCAC | 2 cut(s) | 3471, 4364 |
| ArsI | GACNNNNNNTTYG | 6 cut(s) | 1337, 1369, 1463, 1495, 2555, 2587 |
| AseI | ATTAAT | 1 cut(s) | 3465 |
| AsiGI | ACCGGT | 1 cut(s) | 4269 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1737 |
| AspLEI | GCGC | 9 cut(s) | 61, 257, 286, 295, 303, 322, 478, 520, 4156 |
| AspS9I | GGNCC | 5 cut(s) | 94, 817, 1580, 2171, 3452 |
| AsuC2I | CCSGG | 2 cut(s) | 4328, 4329 |
| AvaI | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| AvaII | GGWCC | 2 cut(s) | 817, 3452 |
| BaeGI | GKGCMC | 4 cut(s) | 398, 2794, 3475, 4368 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2976, 3009 |
| BalI | TGGCCA | 1 cut(s) | 690 |
| BamHI | GGATCC | 3 cut(s) | 3371, 4072, 4735 |
| BanI | GGYRCC | 1 cut(s) | 952 |
| BanII | GRGCYC | 3 cut(s) | 495, 601, 708 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2310, 2342 |
| BauI | CACGAG | 2 cut(s) | 353, 4281 |
| BbrPI | CACGTG | 1 cut(s) | 694 |
| BbsI | GAAGAC | 2 cut(s) | 3371, 4256 |
| Bbv12I | GWGCWC | 6 cut(s) | 495, 708, 3006, 3475, 4368, 4474 |
| BbvCI | CCTCAGC | 1 cut(s) | 323 |
| BceAI | ACGGC | 8 cut(s) | 68, 193, 306, 574, 687, 3322, 3560, 3770 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 4386, 4420 |
| BciT130I | CCWGG | 9 cut(s) | 928, 1892, 2158, 2200, 3492, 3549, 3767, 3938, 4211 |
| BciVI | GTATCC | 2 cut(s) | 4200, 4483 |
| BclI | TGATCA | 2 cut(s) | 1819, 3310 |
| BcnI | CCSGG | 2 cut(s) | 4328, 4329 |
| BcoDI | GTCTC | 7 cut(s) | 732, 1432, 2629, 2740, 2908, 3181, 3721 |
| BfaI | CTAG | 9 cut(s) | 176, 846, 1584, 1682, 2178, 3126, 3386, 3800, 4939 |
| BfmI | CTRYAG | 6 cut(s) | 1638, 1826, 1899, 2276, 4128, 4804 |
| BfoI | RGCGCY | 3 cut(s) | 287, 296, 323 |
| BfrI | CTTAAG | 2 cut(s) | 1160, 3332 |
| BfuAI | ACCTGC | 3 cut(s) | 180, 1676, 2283 |
| BfuI | GTATCC | 2 cut(s) | 4200, 4483 |
| BglII | AGATCT | 1 cut(s) | 2341 |
| BlpI | GCTNAGC | 3 cut(s) | 2663, 3207, 3225 |
| Bme18I | GGWCC | 2 cut(s) | 817, 3452 |
| BmeRI | GACNNNNNGTC | 2 cut(s) | 133, 1660 |
| BmeT110I | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| BmgBI | CACGTC | 1 cut(s) | 750 |
| BmgT120I | GGNCC | 5 cut(s) | 94, 817, 1580, 2171, 3452 |
| BmiI | GGNNCC | 8 cut(s) | 308, 819, 954, 3297, 3373, 3764, 4074, 4737 |
| BmrI | ACTGGG | 4 cut(s) | 1264, 2133, 2709, 3954 |
| BmuI | ACTGGG | 4 cut(s) | 1264, 2133, 2709, 3954 |
| BpiI | GAAGAC | 2 cut(s) | 3371, 4256 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 1481, 1513, 3020, 3052 |
| BpmI | CTGGAG | 4 cut(s) | 2231, 3024, 3048, 3405 |
| Bpu10I | CCTNAGC | 4 cut(s) | 323, 707, 1907, 2414 |
| Bpu1102I | GCTNAGC | 3 cut(s) | 2663, 3207, 3225 |
| BpuEI | CTTGAG | 4 cut(s) | 2456, 3164, 3536, 4135 |
| BpuMI | CCSGG | 2 cut(s) | 4328, 4329 |
| Bsa29I | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BsaAI | YACGTR | 4 cut(s) | 694, 4066, 4494, 4653 |
| BsaBI | GATNNNNATC | 4 cut(s) | 2586, 3525, 4048, 4709 |
| BsaHI | GRCGYC | 3 cut(s) | 129, 416, 881 |
| BsaWI | WCCGGW | 1 cut(s) | 4269 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 69, 99 |
| Bse118I | RCCGGY | 3 cut(s) | 46, 616, 4269 |
| Bse3DI | GCAATG | 2 cut(s) | 973, 4792 |
| Bse8I | GATNNNNATC | 4 cut(s) | 2586, 3525, 4048, 4709 |
| BseBI | CCWGG | 9 cut(s) | 928, 1892, 2158, 2200, 3492, 3549, 3767, 3938, 4211 |
| BseCI | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BseJI | GATNNNNATC | 4 cut(s) | 2586, 3525, 4048, 4709 |
| BseMI | GCAATG | 2 cut(s) | 973, 4792 |
| BseRI | GAGGAG | 4 cut(s) | 662, 907, 2816, 3586 |
| BseSI | GKGCMC | 4 cut(s) | 398, 2794, 3475, 4368 |
| BseX3I | CGGCCG | 1 cut(s) | 851 |
| BseYI | CCCAGC | 3 cut(s) | 96, 2096, 4824 |
| BsgI | GTGCAG | 3 cut(s) | 716, 3263, 3365 |
| Bsh1236I | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| Bsh1285I | CGRYCG | 3 cut(s) | 216, 510, 854 |
| BshNI | GGYRCC | 1 cut(s) | 952 |
| BshTI | ACCGGT | 1 cut(s) | 4269 |
| BshVI | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BsiEI | CGRYCG | 3 cut(s) | 216, 510, 854 |
| BsiHKAI | GWGCWC | 6 cut(s) | 495, 708, 3006, 3475, 4368, 4474 |
| BsiHKCI | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| BsiSI | CCGG | 5 cut(s) | 47, 298, 617, 4270, 4328 |
| BsiWI | CGTACG | 1 cut(s) | 3787 |
| BslFI | GGGAC | 3 cut(s) | 164, 1924, 3372 |
| BsmAI | GTCTC | 7 cut(s) | 732, 1432, 2629, 2740, 2908, 3181, 3721 |
| BsmBI | CGTCTC | 1 cut(s) | 2740 |
| BsmFI | GGGAC | 3 cut(s) | 164, 1924, 3372 |
| BsmI | GAATGC | 4 cut(s) | 278, 1449, 3031, 4826 |
| BsoBI | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| Bsp1286I | GDGCHC | 9 cut(s) | 398, 495, 601, 708, 2794, 3006, 3475, 4368, 4474 |
| Bsp1720I | GCTNAGC | 3 cut(s) | 2663, 3207, 3225 |
| Bsp19I | CCATGG | 1 cut(s) | 1855 |
| BspDI | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BspFNI | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| BspHI | TCATGA | 3 cut(s) | 3013, 4528, 4810 |
| BspLI | GGNNCC | 8 cut(s) | 308, 819, 954, 3297, 3373, 3764, 4074, 4737 |
| BspMAI | CTGCAG | 1 cut(s) | 2280 |
| BspMI | ACCTGC | 3 cut(s) | 180, 1676, 2283 |
| BspPI | GGATC | 9 cut(s) | 617, 1941, 3366, 3379, 3672, 4067, 4080, 4730, 4743 |
| BspQI | GCTCTTC | 1 cut(s) | 2424 |
| BspT107I | GGYRCC | 1 cut(s) | 952 |
| BspTI | CTTAAG | 2 cut(s) | 1160, 3332 |
| BsrDI | GCAATG | 2 cut(s) | 973, 4792 |
| BsrFI | RCCGGY | 3 cut(s) | 46, 616, 4269 |
| BssAI | RCCGGY | 3 cut(s) | 46, 616, 4269 |
| BssNI | GRCGYC | 3 cut(s) | 129, 416, 881 |
| BssSI | CACGAG | 2 cut(s) | 353, 4281 |
| BssT1I | CCWWGG | 3 cut(s) | 1182, 1855, 2598 |
| Bst2BI | CACGAG | 2 cut(s) | 353, 4281 |
| Bst2UI | CCWGG | 9 cut(s) | 928, 1892, 2158, 2200, 3492, 3549, 3767, 3938, 4211 |
| Bst6I | CTCTTC | 9 cut(s) | 771, 882, 974, 2211, 2414, 2424, 3111, 3144, 4135 |
| BstACI | GRCGYC | 3 cut(s) | 129, 416, 881 |
| BstAFI | CTTAAG | 2 cut(s) | 1160, 3332 |
| BstAPI | GCANNNNNTGC | 3 cut(s) | 402, 2867, 3826 |
| BstBAI | YACGTR | 4 cut(s) | 694, 4066, 4494, 4653 |
| BstDSI | CCRYGG | 3 cut(s) | 1855, 2379, 4726 |
| BstEII | GGTNACC | 1 cut(s) | 3056 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 3504, 3779 |
| BstFNI | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| BstH2I | RGCGCY | 3 cut(s) | 287, 296, 323 |
| BstHHI | GCGC | 9 cut(s) | 61, 257, 286, 295, 303, 322, 478, 520, 4156 |
| BstMAI | GTCTC | 7 cut(s) | 732, 1432, 2629, 2740, 2908, 3181, 3721 |
| BstMCI | CGRYCG | 3 cut(s) | 216, 510, 854 |
| BstNI | CCWGG | 9 cut(s) | 928, 1892, 2158, 2200, 3492, 3549, 3767, 3938, 4211 |
| BstNSI | RCATGY | 5 cut(s) | 3010, 3051, 3740, 4061, 4622 |
| BstPI | GGTNACC | 1 cut(s) | 3056 |
| BstSFI | CTRYAG | 6 cut(s) | 1638, 1826, 1899, 2276, 4128, 4804 |
| BstSLI | GKGCMC | 4 cut(s) | 398, 2794, 3475, 4368 |
| BstSNI | TACGTA | 1 cut(s) | 4494 |
| BstUI | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| BstV2I | GAAGAC | 2 cut(s) | 3371, 4256 |
| BstX2I | RGATCY | 5 cut(s) | 1933, 2341, 3371, 4072, 4735 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1949 |
| BstYI | RGATCY | 5 cut(s) | 1933, 2341, 3371, 4072, 4735 |
| BstZI | CGGCCG | 1 cut(s) | 851 |
| Bsu15I | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BsuI | GTATCC | 2 cut(s) | 4200, 4483 |
| BsuTUI | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| BtgI | CCRYGG | 3 cut(s) | 1855, 2379, 4726 |
| BtrI | CACGTC | 1 cut(s) | 750 |
| BtsI | GCAGTG | 3 cut(s) | 1279, 1987, 2053 |
| BtsIMutI | CAGTG | 9 cut(s) | 396, 1217, 1279, 1987, 2053, 4415, 4474, 4682, 4720 |
| BveI | ACCTGC | 3 cut(s) | 180, 1676, 2283 |
| CaiI | CAGNNNCTG | 2 cut(s) | 1936, 3071 |
| CciI | TCATGA | 3 cut(s) | 3013, 4528, 4810 |
| CfoI | GCGC | 9 cut(s) | 61, 257, 286, 295, 303, 322, 478, 520, 4156 |
| Cfr10I | RCCGGY | 3 cut(s) | 46, 616, 4269 |
| Cfr13I | GGNCC | 5 cut(s) | 94, 817, 1580, 2171, 3452 |
| Cfr9I | CCCGGG | 1 cut(s) | 4327 |
| ClaI | ATCGAT | 3 cut(s) | 1109, 2673, 3930 |
| CseI | GACGC | 4 cut(s) | 137, 394, 405, 889 |
| Csp6I | GTAC | 4 cut(s) | 2985, 3788, 3942, 4746 |
| CspAI | ACCGGT | 1 cut(s) | 4269 |
| CviQI | GTAC | 4 cut(s) | 2985, 3788, 3942, 4746 |
| DraI | TTTAAA | 2 cut(s) | 2310, 4524 |
| DraIII | CACNNNGTG | 1 cut(s) | 402 |
| DriI | GACNNNNNGTC | 2 cut(s) | 133, 1660 |
| EaeI | YGGCCR | 5 cut(s) | 549, 688, 851, 1694, 3545 |
| EagI | CGGCCG | 1 cut(s) | 851 |
| Eam1104I | CTCTTC | 9 cut(s) | 771, 882, 974, 2211, 2414, 2424, 3111, 3144, 4135 |
| Eam1105I | GACNNNNNGTC | 2 cut(s) | 133, 1660 |
| EarI | CTCTTC | 9 cut(s) | 771, 882, 974, 2211, 2414, 2424, 3111, 3144, 4135 |
| EciI | GGCGGA | 5 cut(s) | 90, 482, 599, 689, 2728 |
| Ecl136II | GAGCTC | 2 cut(s) | 493, 706 |
| EclXI | CGGCCG | 1 cut(s) | 851 |
| Eco105I | TACGTA | 1 cut(s) | 4494 |
| Eco130I | CCWWGG | 3 cut(s) | 1182, 1855, 2598 |
| Eco24I | GRGCYC | 3 cut(s) | 495, 601, 708 |
| Eco32I | GATATC | 3 cut(s) | 1701, 3928, 4711 |
| Eco47I | GGWCC | 2 cut(s) | 817, 3452 |
| Eco52I | CGGCCG | 1 cut(s) | 851 |
| Eco53kI | GAGCTC | 2 cut(s) | 493, 706 |
| Eco57I | CTGAAG | 2 cut(s) | 2795, 4257 |
| Eco72I | CACGTG | 1 cut(s) | 694 |
| Eco88I | CYCGRG | 3 cut(s) | 181, 370, 4327 |
| Eco91I | GGTNACC | 1 cut(s) | 3056 |
| EcoICRI | GAGCTC | 2 cut(s) | 493, 706 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 3504, 3779 |
| EcoO109I | RGGNCCY | 1 cut(s) | 817 |
| EcoO65I | GGTNACC | 1 cut(s) | 3056 |
| EcoRI | GAATTC | 4 cut(s) | 358, 1737, 2570, 3510 |
| EcoRII | CCWGG | 9 cut(s) | 926, 1890, 2156, 2198, 3490, 3547, 3765, 3936, 4209 |
| EcoRV | GATATC | 3 cut(s) | 1701, 3928, 4711 |
| EcoT14I | CCWWGG | 3 cut(s) | 1182, 1855, 2598 |
| EcoT22I | ATGCAT | 3 cut(s) | 2056, 3012, 3324 |
| EcoT38I | GRGCYC | 3 cut(s) | 495, 601, 708 |
| ErhI | CCWWGG | 3 cut(s) | 1182, 1855, 2598 |
| Esp3I | CGTCTC | 1 cut(s) | 2740 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1122, 1154, 2418, 2450 |
| FaqI | GGGAC | 3 cut(s) | 164, 1924, 3372 |
| FauI | CCCGC | 2 cut(s) | 296, 474 |
| FauNDI | CATATG | 1 cut(s) | 1326 |
| FbaI | TGATCA | 2 cut(s) | 1819, 3310 |
| FblI | GTMKAC | 1 cut(s) | 1637 |
| FriOI | GRGCYC | 3 cut(s) | 495, 601, 708 |
| FspBI | CTAG | 9 cut(s) | 176, 846, 1584, 1682, 2178, 3126, 3386, 3800, 4939 |
| GlaI | GCGC | 9 cut(s) | 60, 256, 285, 294, 302, 321, 477, 519, 4155 |
| GsaI | CCCAGC | 3 cut(s) | 100, 2100, 4828 |
| GsuI | CTGGAG | 4 cut(s) | 2231, 3024, 3048, 3405 |
| HaeII | RGCGCY | 3 cut(s) | 287, 296, 323 |
| HapII | CCGG | 5 cut(s) | 47, 298, 617, 4270, 4328 |
| HgaI | GACGC | 4 cut(s) | 137, 394, 405, 889 |
| HhaI | GCGC | 9 cut(s) | 61, 257, 286, 295, 303, 322, 478, 520, 4156 |
| Hin1I | GRCGYC | 3 cut(s) | 129, 416, 881 |
| Hin6I | GCGC | 9 cut(s) | 59, 255, 284, 293, 301, 320, 476, 518, 4154 |
| HinP1I | GCGC | 9 cut(s) | 59, 255, 284, 293, 301, 320, 476, 518, 4154 |
| HincII | GTYRAC | 4 cut(s) | 1375, 2193, 2512, 3289 |
| HindII | GTYRAC | 4 cut(s) | 1375, 2193, 2512, 3289 |
| HindIII | AAGCTT | 6 cut(s) | 662, 1724, 2398, 3161, 3435, 4517 |
| HpaI | GTTAAC | 2 cut(s) | 2512, 3289 |
| HpaII | CCGG | 5 cut(s) | 47, 298, 617, 4270, 4328 |
| HpyCH4IV | ACGT | 9 cut(s) | 693, 749, 1629, 1717, 2496, 4065, 4160, 4493, 4652 |
| HpySE526I | ACGT | 9 cut(s) | 693, 749, 1629, 1717, 2496, 4065, 4160, 4493, 4652 |
| Hsp92I | GRCGYC | 3 cut(s) | 129, 416, 881 |
| HspAI | GCGC | 9 cut(s) | 59, 255, 284, 293, 301, 320, 476, 518, 4154 |
| KroI | GCCGGC | 1 cut(s) | 46 |
| KroNI | GCCGGC | 1 cut(s) | 48 |
| Ksp22I | TGATCA | 2 cut(s) | 1819, 3310 |
| KspAI | GTTAAC | 2 cut(s) | 2512, 3289 |
| LguI | GCTCTTC | 1 cut(s) | 2424 |
| MaeI | CTAG | 9 cut(s) | 176, 846, 1584, 1682, 2178, 3126, 3386, 3800, 4939 |
| MaeII | ACGT | 9 cut(s) | 693, 749, 1629, 1717, 2496, 4065, 4160, 4493, 4652 |
| MaeIII | GTNAC | 9 cut(s) | 808, 983, 1134, 1706, 2545, 2561, 2695, 3056, 4199 |
| MfeI | CAATTG | 2 cut(s) | 1815, 2711 |
| MflI | RGATCY | 5 cut(s) | 1933, 2341, 3371, 4072, 4735 |
| MhlI | GDGCHC | 9 cut(s) | 398, 495, 601, 708, 2794, 3006, 3475, 4368, 4474 |
| MlsI | TGGCCA | 1 cut(s) | 690 |
| MluNI | TGGCCA | 1 cut(s) | 690 |
| MmeI | TCCRAC | 3 cut(s) | 692, 1226, 2814 |
| Mox20I | TGGCCA | 1 cut(s) | 690 |
| Mph1103I | ATGCAT | 3 cut(s) | 2056, 3012, 3324 |
| MreI | CGCCGGCG | 1 cut(s) | 46 |
| MroNI | GCCGGC | 1 cut(s) | 46 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1737 |
| MscI | TGGCCA | 1 cut(s) | 690 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1876, 1947, 3015, 3584, 4815 |
| Msp20I | TGGCCA | 1 cut(s) | 690 |
| MspA1I | CMGCKG | 4 cut(s) | 349, 448, 715, 1420 |
| MspCI | CTTAAG | 2 cut(s) | 1160, 3332 |
| MspI | CCGG | 5 cut(s) | 47, 298, 617, 4270, 4328 |
| MunI | CAATTG | 2 cut(s) | 1815, 2711 |
| Mva1269I | GAATGC | 4 cut(s) | 278, 1449, 3031, 4826 |
| MvaI | CCWGG | 9 cut(s) | 928, 1892, 2158, 2200, 3492, 3549, 3767, 3938, 4211 |
| MvnI | CGCG | 7 cut(s) | 31, 263, 303, 440, 464, 478, 518 |
| NaeI | GCCGGC | 1 cut(s) | 48 |
| NciI | CCSGG | 2 cut(s) | 4328, 4329 |
| NcoI | CCATGG | 1 cut(s) | 1855 |
| NdeI | CATATG | 1 cut(s) | 1326 |
| NgoMIV | GCCGGC | 1 cut(s) | 46 |
| NlaIV | GGNNCC | 8 cut(s) | 308, 819, 954, 3297, 3373, 3764, 4074, 4737 |
| NmeAIII | GCCGAG | 1 cut(s) | 157 |
| NmuCI | GTSAC | 6 cut(s) | 808, 983, 1706, 2545, 2561, 3056 |
| NsiI | ATGCAT | 3 cut(s) | 2056, 3012, 3324 |
| NspI | RCATGY | 5 cut(s) | 3010, 3051, 3740, 4061, 4622 |
| OliI | CACNNNNGTG | 2 cut(s) | 1876, 3584 |
| PagI | TCATGA | 3 cut(s) | 3013, 4528, 4810 |
| PaqCI | CACCTGC | 2 cut(s) | 1676, 2283 |
| PciI | ACATGT | 1 cut(s) | 4618 |
| PciSI | GCTCTTC | 1 cut(s) | 2424 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 129, 503, 2686 |
| PctI | GAATGC | 4 cut(s) | 278, 1449, 3031, 4826 |
| PdiI | GCCGGC | 1 cut(s) | 48 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1737 |
| Pfl23II | CGTACG | 1 cut(s) | 3787 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1861 |
| PfoI | TCCNGGA | 4 cut(s) | 1890, 2156, 2198, 4209 |
| PinAI | ACCGGT | 1 cut(s) | 4269 |
| Ple19I | CGATCG | 2 cut(s) | 216, 510 |
| PmaCI | CACGTG | 1 cut(s) | 694 |
| PmlI | CACGTG | 1 cut(s) | 694 |
| Ppu21I | YACGTR | 4 cut(s) | 694, 4066, 4494, 4653 |
| PpuMI | RGGWCCY | 1 cut(s) | 817 |
| PscI | ACATGT | 1 cut(s) | 4618 |
| PshBI | ATTAAT | 1 cut(s) | 3465 |
| Psp124BI | GAGCTC | 2 cut(s) | 495, 708 |
| Psp1406I | AACGTT | 1 cut(s) | 2496 |
| Psp5II | RGGWCCY | 1 cut(s) | 817 |
| Psp6I | CCWGG | 9 cut(s) | 926, 1890, 2156, 2198, 3490, 3547, 3765, 3936, 4209 |
| PspCI | CACGTG | 1 cut(s) | 694 |
| PspEI | GGTNACC | 1 cut(s) | 3056 |
| PspFI | CCCAGC | 3 cut(s) | 96, 2096, 4824 |
| PspGI | CCWGG | 9 cut(s) | 926, 1890, 2156, 2198, 3490, 3547, 3765, 3936, 4209 |
| PspLI | CGTACG | 1 cut(s) | 3787 |
| PspN4I | GGNNCC | 8 cut(s) | 308, 819, 954, 3297, 3373, 3764, 4074, 4737 |
| PspPI | GGNCC | 5 cut(s) | 94, 817, 1580, 2171, 3452 |
| PspPPI | RGGWCCY | 1 cut(s) | 817 |
| PstI | CTGCAG | 1 cut(s) | 2280 |
| PstNI | CAGNNNCTG | 2 cut(s) | 1936, 3071 |
| PsuI | RGATCY | 5 cut(s) | 1933, 2341, 3371, 4072, 4735 |
| PvuI | CGATCG | 2 cut(s) | 216, 510 |
| PvuII | CAGCTG | 1 cut(s) | 715 |
| RsaI | GTAC | 4 cut(s) | 2986, 3789, 3943, 4747 |
| RsaNI | GTAC | 4 cut(s) | 2985, 3788, 3942, 4746 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1876, 1947, 3015, 3584, 4815 |
| SacI | GAGCTC | 2 cut(s) | 495, 708 |
| SapI | GCTCTTC | 1 cut(s) | 2424 |
| Sau96I | GGNCC | 5 cut(s) | 94, 817, 1580, 2171, 3452 |
| SduI | GDGCHC | 9 cut(s) | 398, 495, 601, 708, 2794, 3006, 3475, 4368, 4474 |
| SfcI | CTRYAG | 6 cut(s) | 1638, 1826, 1899, 2276, 4128, 4804 |
| SgrAI | CRCCGGYG | 1 cut(s) | 46 |
| SinI | GGWCC | 2 cut(s) | 817, 3452 |
| SmaI | CCCGGG | 1 cut(s) | 4329 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1876, 1947, 3015, 3584, 4815 |
| SmlI | CTYRAG | 6 cut(s) | 1160, 2435, 3143, 3332, 3515, 4114 |
| SmoI | CTYRAG | 6 cut(s) | 1160, 2435, 3143, 3332, 3515, 4114 |
| SnaBI | TACGTA | 1 cut(s) | 4494 |
| SspI | AATATT | 2 cut(s) | 3631, 4104 |
| SspMI | CTAG | 9 cut(s) | 176, 846, 1584, 1682, 2178, 3126, 3386, 3800, 4939 |
| SstI | GAGCTC | 2 cut(s) | 495, 708 |
| StyI | CCWWGG | 3 cut(s) | 1182, 1855, 2598 |
| TaiI | ACGT | 9 cut(s) | 696, 752, 1632, 1720, 2499, 4068, 4163, 4496, 4655 |
| TaqII | GACCGA | 1 cut(s) | 2742 |
| TatI | WGTACW | 1 cut(s) | 4745 |
| TscAI | CASTG | 9 cut(s) | 403, 1224, 1279, 1987, 2053, 4415, 4474, 4689, 4720 |
| TseFI | GTSAC | 6 cut(s) | 808, 983, 1706, 2545, 2561, 3056 |
| Tsp45I | GTSAC | 6 cut(s) | 808, 983, 1706, 2545, 2561, 3056 |
| TspGWI | ACGGA | 4 cut(s) | 2396, 2695, 3145, 3609 |
| TspMI | CCCGGG | 1 cut(s) | 4327 |
| TspRI | CASTG | 9 cut(s) | 403, 1224, 1279, 1987, 2053, 4415, 4474, 4689, 4720 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1861 |
| Vha464I | CTTAAG | 2 cut(s) | 1160, 3332 |
| VneI | GTGCAC | 2 cut(s) | 3471, 4364 |
| VpaK11BI | GGWCC | 2 cut(s) | 817, 3452 |
| VspI | ATTAAT | 1 cut(s) | 3465 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 3504, 3779 |
| XbaI | TCTAGA | 2 cut(s) | 3125, 3385 |
| XceI | RCATGY | 5 cut(s) | 3010, 3051, 3740, 4061, 4622 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1189 |
| XmaI | CCCGGG | 1 cut(s) | 4327 |
| XmiI | GTMKAC | 1 cut(s) | 1637 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1737 |
| XspI | CTAG | 9 cut(s) | 176, 846, 1584, 1682, 2178, 3126, 3386, 3800, 4939 |
| Zsp2I | ATGCAT | 3 cut(s) | 2056, 3012, 3324 |