Rmu_sc0004223.1_g000013

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004223.1
OG0018446
Physical Location & Seq
Forward (+)
56999 .. 57472
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004223.1_g000013.1.cds

Sequence Viewer

Length: 474 bp
atgtcgaatgaacctagactcgactttcccatgcttgactcaacaggctcggattaccatagttgggtaaactacgttgagaaccatctcacttcaaagggaatattacccataatccaagcacctaacccggatcttgtgttcatacgaacacctacaaagcatgctcaagcagttatcttgatgcgacgccatatggacaaggcactcagattggagtatatgtcgatcaaaaatgcaagagagctatgggtagcgctagaagaacgctttggcaatgtccaagatttcctcctccctcacttgaaggttcaatggaacaatctgtgctttgctgaatatactgttgctgaatataatttagaagctcttcgcatacagtccatgttgaggttttgtagacaacctgtcacagagcaagagctaattgagaaaactctctccaccttccctttttcagccattgtggtatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Retrotran_gag_2 zf-CCHC

Protein Analysis

157

Amino Acids

18.35

Weight (kDa)

6.2

Isoelectric Point (pI)

49.19

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 400
AclWI GGATC 1 cut(s) 141
AcyI GRCGYC 1 cut(s) 190
AfeI AGCGCT 1 cut(s) 258
AfiI CCNNNNNNNGG 2 cut(s) 64, 390
AgsI TTSAA 3 cut(s) 96, 307, 314
AhdI GACNNNNNGTC 1 cut(s) 407
AjuI GAANNNNNNNTTGG 2 cut(s) 255, 287
AloI GAACNNNNNNTCC 2 cut(s) 125, 157
AluBI AGCT 3 cut(s) 247, 368, 424
AluI AGCT 3 cut(s) 247, 368, 424
AlwI GGATC 1 cut(s) 141
Aor51HI AGCGCT 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 369
AspLEI GCGC 1 cut(s) 259
AsuC2I CCSGG 1 cut(s) 131
BccI CCATC 1 cut(s) 93
BcnI CCSGG 1 cut(s) 131
BfaI CTAG 2 cut(s) 15, 260
BfoI RGCGCY 1 cut(s) 260
Bme1390I CCNGG 1 cut(s) 131
BmeRI GACNNNNNGTC 1 cut(s) 407
BmrFI CCNGG 1 cut(s) 131
BmsI GCATC 1 cut(s) 174
BpuEI CTTGAG 1 cut(s) 153
BpuMI CCSGG 1 cut(s) 131
BsaHI GRCGYC 1 cut(s) 190
BsaXI ACNNNNNCTCC 2 cut(s) 209, 239
Bsc4I CCNNNNNNNGG 2 cut(s) 64, 390
Bse3DI GCAATG 1 cut(s) 283
BseLI CCNNNNNNNGG 2 cut(s) 64, 390
BseMI GCAATG 1 cut(s) 283
BseMII CTCAG 1 cut(s) 223
BseRI GAGGAG 1 cut(s) 284
BsiSI CCGG 1 cut(s) 131
BslI CCNNNNNNNGG 2 cut(s) 64, 390
Bsp143I GATC 2 cut(s) 133, 228
BspCNI CTCAG 1 cut(s) 222
BspPI GGATC 1 cut(s) 141
BspQI GCTCTTC 1 cut(s) 375
BsrDI GCAATG 1 cut(s) 283
BssMI GATC 2 cut(s) 133, 228
BssNI GRCGYC 1 cut(s) 190
Bst4CI ACNGT 2 cut(s) 346, 381
Bst6I CTCTTC 1 cut(s) 375
BstACI GRCGYC 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 165
BstDEI CTNAG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 260
BstHHI GCGC 1 cut(s) 259
BstKTI GATC 2 cut(s) 136, 231
BstMBI GATC 2 cut(s) 133, 228
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 129
BstX2I RGATCY 1 cut(s) 133
BstYI RGATCY 1 cut(s) 133
Cac8I GCNNGC 1 cut(s) 165
CfoI GCGC 1 cut(s) 259
CseI GACGC 1 cut(s) 198
CviAII CATG 3 cut(s) 31, 164, 385
CviJI RGCY 5 cut(s) 48, 247, 368, 424, 461
CviKI_1 RGCY 5 cut(s) 48, 247, 368, 424, 461
DdeI CTNAG 1 cut(s) 209
DpnI GATC 2 cut(s) 135, 230
DpnII GATC 2 cut(s) 133, 228
DriI GACNNNNNGTC 1 cut(s) 407
Eam1104I CTCTTC 1 cut(s) 375
Eam1105I GACNNNNNGTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 375
Eco47III AGCGCT 1 cut(s) 258
FaeI CATG 3 cut(s) 34, 167, 388
FatI CATG 3 cut(s) 30, 163, 384
FauNDI CATATG 1 cut(s) 195
FblI GTMKAC 1 cut(s) 400
FspBI CTAG 2 cut(s) 15, 260
GlaI GCGC 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 260
HapII CCGG 1 cut(s) 131
HgaI GACGC 1 cut(s) 198
HhaI GCGC 1 cut(s) 259
Hin1I GRCGYC 1 cut(s) 190
Hin1II CATG 3 cut(s) 34, 167, 388
Hin6I GCGC 1 cut(s) 257
HinP1I GCGC 1 cut(s) 257
HinfI GANTC 2 cut(s) 18, 38
HpaII CCGG 1 cut(s) 131
Hpy166II GTNNAC 2 cut(s) 70, 401
Hpy188I TCNGA 2 cut(s) 52, 212
Hpy188III TCNNGA 1 cut(s) 181
Hpy8I GTNNAC 2 cut(s) 70, 401
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 2 cut(s) 301, 457
HpyCH4III ACNGT 2 cut(s) 346, 381
HpyCH4IV ACGT 1 cut(s) 75
HpyCH4V TGCA 1 cut(s) 239
HpyF3I CTNAG 1 cut(s) 209
HpySE526I ACGT 1 cut(s) 75
Hsp92I GRCGYC 1 cut(s) 190
Hsp92II CATG 3 cut(s) 34, 167, 388
HspAI GCGC 1 cut(s) 257
Kzo9I GATC 2 cut(s) 133, 228
LguI GCTCTTC 1 cut(s) 375
LpnPI CCDG 3 cut(s) 30, 144, 420
LweI GCATC 1 cut(s) 174
MaeI CTAG 2 cut(s) 15, 260
MaeII ACGT 1 cut(s) 75
MaeIII GTNAC 1 cut(s) 409
MalI GATC 2 cut(s) 135, 230
MboI GATC 2 cut(s) 133, 228
MboII GAAGA 2 cut(s) 275, 362
MflI RGATCY 1 cut(s) 133
MluCI AATT 2 cut(s) 358, 426
MlyI GAGTC 2 cut(s) 12, 32
MnlI CCTC 4 cut(s) 302, 305, 309, 384
MroXI GAANNNNTTC 1 cut(s) 369
MspI CCGG 1 cut(s) 131
MspR9I CCNGG 1 cut(s) 131
NciI CCSGG 1 cut(s) 131
NdeI CATATG 1 cut(s) 195
NdeII GATC 2 cut(s) 133, 228
NlaIII CATG 3 cut(s) 34, 167, 388
NmuCI GTSAC 1 cut(s) 409
NspI RCATGY 1 cut(s) 167
PaeI GCATGC 1 cut(s) 167
PciSI GCTCTTC 1 cut(s) 375
PdmI GAANNNNTTC 1 cut(s) 369
PleI GAGTC 2 cut(s) 12, 32
PpsI GAGTC 2 cut(s) 12, 32
PsuI RGATCY 1 cut(s) 133
SapI GCTCTTC 1 cut(s) 375
Sau3AI GATC 2 cut(s) 133, 228
SchI GAGTC 2 cut(s) 12, 32
ScrFI CCNGG 1 cut(s) 131
SfaNI GCATC 1 cut(s) 174
SmlI CTYRAG 1 cut(s) 168
SmoI CTYRAG 1 cut(s) 168
SphI GCATGC 1 cut(s) 167
Sse9I AATT 2 cut(s) 358, 426
SspI AATATT 1 cut(s) 105
SspMI CTAG 2 cut(s) 15, 260
StyD4I CCNGG 1 cut(s) 129
TaaI ACNGT 2 cut(s) 346, 381
TaiI ACGT 1 cut(s) 78
TaqI TCGA 3 cut(s) 5, 21, 227
TasI AATT 2 cut(s) 358, 426
TseFI GTSAC 1 cut(s) 409
Tsp45I GTSAC 1 cut(s) 409
TspDTI ATGAA 2 cut(s) 24, 133
XceI RCATGY 1 cut(s) 167
XmiI GTMKAC 1 cut(s) 400
XmnI GAANNNNTTC 1 cut(s) 369
XspI CTAG 2 cut(s) 15, 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.