Rmu_sc0004296.1_g000013

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004296.1
OG0018343
Physical Location & Seq
Reverse (-)
45720 .. 49286
3567 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
atgaacctgcctacgatccgtagatgcagttctgtccataacatcgttgaaaagttcatcaagaagcgttacagaaagaagttggacatgttgacgaatgaagaagatgaacaagaagaggaatatctgcaagctaggcaggttaaaagacgaattttccctgaagacacagcagccatacaaaagtacacaacgaaacgaaagttaacgaatttacatgaagacgaagaggaagaggaagagatcacatgggtcaaacgacgaaagactttggagcacattggcaaggaaatcaaagccttgaggataactacaagacaagaatatggtacgagttgcaatatagatttcgcaagtcttcctccagatattgtgcttcttattctggataagttgttagaacccattgaccatgctcgttttgctgtcgtctgtattgctgtcgtctgtaaggaatggcaatctcttgcaaaacagtataattgtgaaacgcagccctgggttaaggtcctccctcccatgctatggatccctacccaatctcatggctccgatgacccaatgaccatgctttacagcttgtctgaaagaaaaatctacagaaatatgaagttgccagtgcctcgtgatgataggatctattgtggttatggatggatggctaagtatcgcttatttagtgtaaagcaagtcgtgaagataaccttgcctgatcctactttggatcggaacaattatgtggttctagtcatatgcggttctagactgtacttcatcagaggaggacaaaaactttggagtcccatgcatacgtggtgcagtgctgcaacatattatgaagggcgagtgttctcagttcgtggttttggaagagatatcccagggcacgagcttgtatcatactgcgttgatagcagcgttgacatatattacagtagtttacatctgcaacatgtggcatggcttgaacattgtactctatcccatcttctaattccaacatatttcgtaacatcaacaaagggggatttactggttgttgagagattgttggaagaagacagattcaacatcttcaagcggcggaaaatgctgaggttcaaggttttcaagtgggttgaatccattgcacagctagctgaggtaaatgacattggggatgaggctattttcgtgggcaacaatcaatcggtatgtgttttggcttcaaagtttcccgggttcaacccgctttatttcgtgcgggtttctagtcgtgttatcgggtcgtgtcgtgtcgtgtcggaaatgacagccctaatcacaacattgttcattttcggctccagtttctccaaaaatggaagaaagaaaagtgatggaggcttgacaagtgtcacaatttcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

462

Amino Acids

53.59

Weight (kDa)

8.96

Isoelectric Point (pI)

44.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_KIB1-4 PF03478 205 - 410 2.8e-18 KIB1-4 beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06764 FvH4_1g06765
rosa_laevigata RLG00000016319
rosa_multiflora Rmu_sc0002174.1_g000002 Rmu_sc0002503.1_g000018 Rmu_sc0004296.1_g000013
rosa_samantha Rh2AG073000 Rh2BG073700 Rh2CG074800 Rh2DG071800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 15, 130
AccB7I CCANNNNNTGG 1 cut(s) 525
AciI CCGC 5 cut(s) 756, 1081, 1084, 1229, 1243
AclWI GGATC 6 cut(s) 10, 523, 536, 644, 707, 732
AcsI RAATTY 2 cut(s) 153, 211
AcuI CTGAAG 1 cut(s) 183
AfaI GTAC 4 cut(s) 188, 331, 770, 976
AfiI CCNNNNNNNGG 1 cut(s) 525
AflIII ACRYGT 2 cut(s) 87, 952
AgsI TTSAA 9 cut(s) 50, 968, 1069, 1078, 1102, 1111, 1121, 1209, 1225
AjnI CCWGG 2 cut(s) 497, 880
AluBI AGCT 5 cut(s) 134, 579, 892, 1135, 1139
AluI AGCT 5 cut(s) 134, 579, 892, 1135, 1139
Alw21I GWGCWC 1 cut(s) 279
AlwI GGATC 6 cut(s) 10, 523, 536, 644, 707, 732
Ama87I CYCGRG 1 cut(s) 1217
ApeKI GCWGC 4 cut(s) 173, 493, 824, 915
ApoI RAATTY 2 cut(s) 153, 211
ArsI GACNNNNNNTTYG 2 cut(s) 777, 809
AspS9I GGNCC 1 cut(s) 508
AsuC2I CCSGG 2 cut(s) 1218, 1219
AsuNHI GCTAGC 1 cut(s) 1135
AvaI CYCGRG 1 cut(s) 1217
AvaII GGWCC 1 cut(s) 508
BaeGI GKGCMC 1 cut(s) 888
BamHI GGATCC 1 cut(s) 528
BauI CACGAG 2 cut(s) 624, 887
BbsI GAAGAC 4 cut(s) 171, 228, 350, 1065
Bbv12I GWGCWC 1 cut(s) 279
BbvCI CCTCAGC 2 cut(s) 1094, 1140
BbvI GCAGC 4 cut(s) 185, 505, 811, 927
BccI CCATC 4 cut(s) 648, 652, 993, 1352
BciT130I CCWGG 2 cut(s) 499, 882
BcnI CCSGG 2 cut(s) 1218, 1219
BfaI CTAG 5 cut(s) 135, 746, 762, 1136, 1251
BfmI CTRYAG 1 cut(s) 598
BfuAI ACCTGC 2 cut(s) 15, 130
BisI GCNGC 5 cut(s) 174, 494, 825, 916, 1082
BlsI GCNGC 5 cut(s) 175, 495, 826, 917, 1083
Bme1390I CCNGG 4 cut(s) 499, 882, 1218, 1219
Bme18I GGWCC 1 cut(s) 508
BmeT110I CYCGRG 1 cut(s) 1217
BmgT120I GGNCC 1 cut(s) 508
BmiI GGNNCC 3 cut(s) 530, 550, 1324
BmrFI CCNGG 4 cut(s) 499, 882, 1218, 1219
BmsI GCATC 1 cut(s) 14
BmtI GCTAGC 1 cut(s) 1139
BoxI GACNNNNGTC 1 cut(s) 1373
BpiI GAAGAC 4 cut(s) 171, 228, 350, 1065
BpmI CTGGAG 2 cut(s) 348, 1309
Bpu10I CCTNAGC 2 cut(s) 1094, 1140
BpuEI CTTGAG 1 cut(s) 322
BpuMI CCSGG 2 cut(s) 1218, 1219
BsaAI YACGTR 1 cut(s) 813
BsaJI CCNNGG 5 cut(s) 497, 498, 880, 881, 1217
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse1I ACTGG 3 cut(s) 617, 1038, 1326
Bse3DI GCAATG 1 cut(s) 1125
BseBI CCWGG 2 cut(s) 499, 882
BseDI CCNNGG 5 cut(s) 497, 498, 880, 881, 1217
BseGI GGATG 3 cut(s) 659, 663, 1165
BseLI CCNNNNNNNGG 1 cut(s) 525
BseMI GCAATG 1 cut(s) 1125
BseMII CTCAG 3 cut(s) 867, 1085, 1131
BseNI ACTGG 3 cut(s) 617, 1038, 1326
BseRI GAGGAG 1 cut(s) 795
BseSI GKGCMC 1 cut(s) 888
BseXI GCAGC 4 cut(s) 185, 505, 811, 927
BsgI GTGCAG 1 cut(s) 838
BsiHKAI GWGCWC 1 cut(s) 279
BsiHKCI CYCGRG 1 cut(s) 1217
BsiSI CCGG 1 cut(s) 1218
BslFI GGGAC 1 cut(s) 786
BslI CCNNNNNNNGG 1 cut(s) 525
BsmFI GGGAC 1 cut(s) 786
BsoBI CYCGRG 1 cut(s) 1217
Bsp1286I GDGCHC 2 cut(s) 279, 888
Bsp143I GATC 6 cut(s) 15, 243, 528, 636, 712, 724
BspACI CCGC 5 cut(s) 756, 1081, 1084, 1229, 1243
BspCNI CTCAG 3 cut(s) 866, 1086, 1132
BspLI GGNNCC 3 cut(s) 530, 550, 1324
BspMI ACCTGC 2 cut(s) 15, 130
BspOI GCTAGC 1 cut(s) 1139
BspPI GGATC 6 cut(s) 10, 523, 536, 644, 707, 732
BsrDI GCAATG 1 cut(s) 1125
BsrI ACTGG 3 cut(s) 617, 1038, 1326
BssECI CCNNGG 5 cut(s) 497, 498, 880, 881, 1217
BssMI GATC 6 cut(s) 15, 243, 528, 636, 712, 724
BssSI CACGAG 2 cut(s) 624, 887
Bst2BI CACGAG 2 cut(s) 624, 887
Bst2UI CCWGG 2 cut(s) 499, 882
Bst4CI ACNGT 3 cut(s) 477, 768, 935
Bst6I CTCTTC 5 cut(s) 111, 222, 228, 234, 865
BstBAI YACGTR 1 cut(s) 813
BstC8I GCNNGC 2 cut(s) 132, 1137
BstDEI CTNAG 5 cut(s) 663, 853, 1094, 1140, 1386
BstF5I GGATG 3 cut(s) 659, 663, 1165
BstKTI GATC 6 cut(s) 18, 246, 531, 639, 715, 727
BstMBI GATC 6 cut(s) 15, 243, 528, 636, 712, 724
BstMWI GCNNNNNNNGC 5 cut(s) 136, 422, 912, 1090, 1136
BstNI CCWGG 2 cut(s) 499, 882
BstNSI RCATGY 2 cut(s) 91, 956
BstPAI GACNNNNGTC 1 cut(s) 1373
BstSCI CCNGG 4 cut(s) 497, 880, 1216, 1217
BstSFI CTRYAG 1 cut(s) 598
BstSLI GKGCMC 1 cut(s) 888
BstV1I GCAGC 4 cut(s) 185, 505, 811, 927
BstV2I GAAGAC 4 cut(s) 171, 228, 350, 1065
BstX2I RGATCY 2 cut(s) 528, 636
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 2 cut(s) 528, 636
BtsCI GGATG 3 cut(s) 659, 663, 1165
BtsI GCAGTG 1 cut(s) 826
BtsIMutI CAGTG 2 cut(s) 624, 826
BveI ACCTGC 2 cut(s) 15, 130
Cac8I GCNNGC 2 cut(s) 132, 1137
Cfr13I GGNCC 1 cut(s) 508
Cfr9I CCCGGG 1 cut(s) 1217
Csp6I GTAC 4 cut(s) 187, 330, 769, 975
CviQI GTAC 4 cut(s) 187, 330, 769, 975
DdeI CTNAG 5 cut(s) 663, 853, 1094, 1140, 1386
DpnI GATC 6 cut(s) 17, 245, 530, 638, 714, 726
DpnII GATC 6 cut(s) 15, 243, 528, 636, 712, 724
Eam1104I CTCTTC 5 cut(s) 111, 222, 228, 234, 865
EarI CTCTTC 5 cut(s) 111, 222, 228, 234, 865
EciI GGCGGA 1 cut(s) 1099
Eco32I GATATC 1 cut(s) 877
Eco47I GGWCC 1 cut(s) 508
Eco57I CTGAAG 1 cut(s) 183
Eco88I CYCGRG 1 cut(s) 1217
EcoO109I RGGNCCY 1 cut(s) 508
EcoRII CCWGG 2 cut(s) 497, 880
EcoRV GATATC 1 cut(s) 877
EcoT22I ATGCAT 1 cut(s) 810
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 689, 721
FaqI GGGAC 1 cut(s) 786
FauI CCCGC 2 cut(s) 1236, 1236
FauNDI CATATG 1 cut(s) 752
Fnu4HI GCNGC 5 cut(s) 174, 494, 825, 916, 1082
FokI GGATG 3 cut(s) 666, 670, 1172
Fsp4HI GCNGC 5 cut(s) 174, 494, 825, 916, 1082
FspBI CTAG 5 cut(s) 135, 746, 762, 1136, 1251
GluI GCNGC 5 cut(s) 174, 494, 825, 916, 1082
GsuI CTGGAG 2 cut(s) 348, 1309
HapII CCGG 1 cut(s) 1218
HincII GTYRAC 3 cut(s) 93, 207, 922
HindII GTYRAC 3 cut(s) 93, 207, 922
HinfI GANTC 3 cut(s) 799, 1065, 1121
HpaI GTTAAC 1 cut(s) 207
HpaII CCGG 1 cut(s) 1218
Hpy166II GTNNAC 5 cut(s) 93, 189, 207, 922, 941
Hpy188I TCNGA 5 cut(s) 553, 586, 729, 779, 1285
Hpy188III TCNNGA 6 cut(s) 61, 365, 386, 626, 694, 762
Hpy8I GTNNAC 5 cut(s) 93, 189, 207, 922, 941
Hpy99I CGWCG 1 cut(s) 264
HpyAV CCTTC 1 cut(s) 833
HpyCH4III ACNGT 3 cut(s) 477, 768, 935
HpyCH4IV ACGT 1 cut(s) 812
HpyCH4V TGCA 9 cut(s) 27, 130, 339, 470, 808, 819, 827, 949, 1130
HpyF10VI GCNNNNNNNGC 5 cut(s) 136, 422, 912, 1090, 1136
HpyF3I CTNAG 5 cut(s) 663, 853, 1094, 1140, 1386
HpySE526I ACGT 1 cut(s) 812
KspAI GTTAAC 1 cut(s) 207
Kzo9I GATC 6 cut(s) 15, 243, 528, 636, 712, 724
LmnI GCTCC 3 cut(s) 274, 554, 1328
Lsp1109I GCAGC 4 cut(s) 185, 505, 811, 927
LweI GCATC 1 cut(s) 14
MaeI CTAG 5 cut(s) 135, 746, 762, 1136, 1251
MaeII ACGT 1 cut(s) 812
MaeIII GTNAC 3 cut(s) 68, 1009, 1375
MalI GATC 6 cut(s) 17, 245, 530, 638, 714, 726
MboI GATC 6 cut(s) 15, 243, 528, 636, 712, 724
MflI RGATCY 2 cut(s) 528, 636
MhlI GDGCHC 2 cut(s) 279, 888
MluCI AATT 6 cut(s) 153, 211, 481, 733, 993, 1380
MlyI GAGTC 1 cut(s) 808
MmeI TCCRAC 4 cut(s) 63, 1022, 1032, 1263
Mph1103I ATGCAT 1 cut(s) 810
MseI TTAA 3 cut(s) 144, 206, 504
MspI CCGG 1 cut(s) 1218
MspR9I CCNGG 4 cut(s) 499, 882, 1218, 1219
MvaI CCWGG 2 cut(s) 499, 882
MwoI GCNNNNNNNGC 5 cut(s) 136, 422, 912, 1090, 1136
NciI CCSGG 2 cut(s) 1218, 1219
NdeI CATATG 1 cut(s) 752
NdeII GATC 6 cut(s) 15, 243, 528, 636, 712, 724
NheI GCTAGC 1 cut(s) 1135
NlaIV GGNNCC 3 cut(s) 530, 550, 1324
NmuCI GTSAC 1 cut(s) 1375
NsiI ATGCAT 1 cut(s) 810
NspI RCATGY 2 cut(s) 91, 956
PasI CCCWGGG 2 cut(s) 498, 881
PciI ACATGT 2 cut(s) 87, 952
PfeI GAWTC 2 cut(s) 1065, 1121
PflMI CCANNNNNTGG 1 cut(s) 525
PkrI GCNGC 5 cut(s) 175, 495, 826, 917, 1083
PleI GAGTC 1 cut(s) 807
PpsI GAGTC 1 cut(s) 807
Ppu21I YACGTR 1 cut(s) 813
PpuMI RGGWCCY 1 cut(s) 508
PscI ACATGT 2 cut(s) 87, 952
PshAI GACNNNNGTC 1 cut(s) 1373
Psp5II RGGWCCY 1 cut(s) 508
Psp6I CCWGG 2 cut(s) 497, 880
PspGI CCWGG 2 cut(s) 497, 880
PspN4I GGNNCC 3 cut(s) 530, 550, 1324
PspPI GGNCC 1 cut(s) 508
PspPPI RGGWCCY 1 cut(s) 508
PsrI GAACNNNNNNTAC 2 cut(s) 13, 45
PsuI RGATCY 2 cut(s) 528, 636
RsaI GTAC 4 cut(s) 188, 331, 770, 976
RsaNI GTAC 4 cut(s) 187, 330, 769, 975
SaqAI TTAA 3 cut(s) 144, 206, 504
SatI GCNGC 5 cut(s) 174, 494, 825, 916, 1082
Sau3AI GATC 6 cut(s) 15, 243, 528, 636, 712, 724
Sau96I GGNCC 1 cut(s) 508
SchI GAGTC 1 cut(s) 808
ScrFI CCNGG 4 cut(s) 499, 882, 1218, 1219
SduI GDGCHC 2 cut(s) 279, 888
SfaNI GCATC 1 cut(s) 14
SfcI CTRYAG 1 cut(s) 598
SinI GGWCC 1 cut(s) 508
SmaI CCCGGG 1 cut(s) 1219
SmlI CTYRAG 1 cut(s) 301
SmoI CTYRAG 1 cut(s) 301
Sse9I AATT 6 cut(s) 153, 211, 481, 733, 993, 1380
SsiI CCGC 5 cut(s) 756, 1081, 1084, 1229, 1243
SspMI CTAG 5 cut(s) 135, 746, 762, 1136, 1251
StyD4I CCNGG 4 cut(s) 497, 880, 1216, 1217
TaaI ACNGT 3 cut(s) 477, 768, 935
TaiI ACGT 1 cut(s) 815
TasI AATT 6 cut(s) 153, 211, 481, 733, 993, 1380
TatI WGTACW 3 cut(s) 186, 768, 974
TauI GCSGC 1 cut(s) 1084
TfiI GAWTC 2 cut(s) 1065, 1121
Tru1I TTAA 3 cut(s) 144, 206, 504
Tru9I TTAA 3 cut(s) 144, 206, 504
TscAI CASTG 2 cut(s) 624, 826
TseFI GTSAC 1 cut(s) 1375
TseI GCWGC 4 cut(s) 173, 493, 824, 915
Tsp45I GTSAC 1 cut(s) 1375
TspDTI ATGAA 9 cut(s) 17, 46, 114, 123, 234, 623, 763, 852, 1303
TspGWI ACGGA 1 cut(s) 8
TspMI CCCGGG 1 cut(s) 1217
TspRI CASTG 2 cut(s) 624, 826
Van91I CCANNNNNTGG 1 cut(s) 525
VpaK11BI GGWCC 1 cut(s) 508
XapI RAATTY 2 cut(s) 153, 211
XbaI TCTAGA 1 cut(s) 761
XceI RCATGY 2 cut(s) 91, 956
XmaI CCCGGG 1 cut(s) 1217
XspI CTAG 5 cut(s) 135, 746, 762, 1136, 1251
Zsp2I ATGCAT 1 cut(s) 810
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.