Rh2BG073700

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018343
Physical Location & Seq
Forward (+)
5565289 .. 5566227
939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG073700.1

Sequence Viewer

Length: 939 bp
ATGGGGATTATGAACCTGCCTACGATCCGTAGATGCAGTTCTGTCCATAACATTGTTGAAAAGTTCATCAAGAAGCGTTACAGAAAGAAGTTGGACATGTTGACGAATGAAGAAGATGAACAAGAAGAGGAATATCTGCAAGCTAGGCAGGTTAAAAGAAGAATTTTCCCTGAAGTAGAAGCTGAACTTGCCCGGCTGATGATGAAGTTGAACTTGCCCTGCGGACGGGTTCAAAGAGAGTGGGAGCAGGACACTGCAGCCATACAAAAGTACACAACGAAACGAAAGTTAACTAATTTACATGAAGACGAAGAGGAAGAGGAAGAGATTACATGGGTCAAACGACGAAAGACTTTGGGCAAGGAAATCAAAGCCTTGAGGGTAACTACAAGACAAGAATATGATACGAGTTGCAATATAGATTTCGCAAGTCTTCCTCCAGATATTGTGCTTCTTATTCTGGATAAGTTGTTAGAACCCATTGACCATGCTCGTTTTGCTGTCGTCTGTAAGGAATGGCAGTCTCTTGCAAAACAGTATAATTGTGACACGCAGCCCTGGGTTAAGGTCCTCCCTCCCATGCTATGGATCCCTACCCAATCTCATGGCTCCGATGACCCAATGACCATGCTTTACAGCTTGTCTGAAAGAAAAATCTACAGAAATATGAAGTTGCCAGTGCCTCGTGATGATAGGATCTATTGTGGTTATGGATGGATGGCTAAGTATCGGTATCTAAATAAGTCCTCAGATTATTATGCTAGTCTAGCAATAACTCTCATGAATCCTTTCAGAAACGCAAAAGATATTATCCGTCTCCCTCCAATCCAAGACAGCTTGTTTAGTGTAAAGCAAGTCGTGAAGATAACCACTACTACAAAAGAGGCCTATGGGGACACATATATGGGGACACTTGTTGTATGTGTGCCCATTGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF295 F-box F-box-like

Protein Analysis

312

Amino Acids

36.7

Weight (kDa)

8.79

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box-like PF12937 142 - 178 2.7e-07 F-box-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018343)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06764 FvH4_1g06765
rosa_laevigata RLG00000016319
rosa_multiflora Rmu_sc0002174.1_g000002 Rmu_sc0002503.1_g000018 Rmu_sc0004296.1_g000013
rosa_samantha Rh2AG073000 Rh2BG073700 Rh2CG074800 Rh2DG071800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 24, 139
AccB7I CCANNNNNTGG 1 cut(s) 585
AciI CCGC 1 cut(s) 222
AclWI GGATC 4 cut(s) 19, 583, 596, 704
AcsI RAATTY 1 cut(s) 162
AcuI CTGAAG 1 cut(s) 192
AfaI GTAC 1 cut(s) 272
AfiI CCNNNNNNNGG 2 cut(s) 225, 585
AflIII ACRYGT 1 cut(s) 96
AgsI TTSAA 3 cut(s) 59, 211, 233
AjnI CCWGG 1 cut(s) 557
AluBI AGCT 4 cut(s) 143, 182, 639, 837
AluI AGCT 4 cut(s) 143, 182, 639, 837
Alw26I GTCTC 2 cut(s) 528, 821
AlwI GGATC 4 cut(s) 19, 583, 596, 704
AoxI GGCC 1 cut(s) 885
ApeKI GCWGC 2 cut(s) 257, 553
ApoI RAATTY 1 cut(s) 162
Asp700I GAANNNNTTC 1 cut(s) 788
AspS9I GGNCC 1 cut(s) 568
AsuC2I CCSGG 1 cut(s) 193
AvaII GGWCC 1 cut(s) 568
BaeGI GKGCMC 1 cut(s) 930
BamHI GGATCC 1 cut(s) 588
BauI CACGAG 1 cut(s) 684
BbsI GAAGAC 2 cut(s) 312, 425
BbvI GCAGC 2 cut(s) 269, 565
BccI CCATC 2 cut(s) 708, 712
BciT130I CCWGG 1 cut(s) 559
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 528, 821
BfaI CTAG 3 cut(s) 144, 762, 767
BfmI CTRYAG 2 cut(s) 255, 658
BfuAI ACCTGC 2 cut(s) 24, 139
BisI GCNGC 2 cut(s) 258, 554
BlsI GCNGC 2 cut(s) 259, 555
Bme1390I CCNGG 2 cut(s) 193, 559
Bme18I GGWCC 1 cut(s) 568
BmgT120I GGNCC 1 cut(s) 568
BmiI GGNNCC 2 cut(s) 590, 610
BmrFI CCNGG 2 cut(s) 193, 559
BmsI GCATC 1 cut(s) 23
BpiI GAAGAC 2 cut(s) 312, 425
BpmI CTGGAG 1 cut(s) 423
BpuEI CTTGAG 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 193
BsaJI CCNNGG 2 cut(s) 557, 558
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 585
Bse1I ACTGG 1 cut(s) 677
Bse3DI GCAATG 1 cut(s) 930
BseBI CCWGG 1 cut(s) 559
BseDI CCNNGG 2 cut(s) 557, 558
BseGI GGATG 2 cut(s) 719, 723
BseLI CCNNNNNNNGG 2 cut(s) 225, 585
BseMI GCAATG 1 cut(s) 930
BseMII CTCAG 1 cut(s) 762
BseNI ACTGG 1 cut(s) 677
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 2 cut(s) 269, 565
BshFI GGCC 1 cut(s) 887
BsiSI CCGG 1 cut(s) 193
BslFI GGGAC 2 cut(s) 908, 922
BslI CCNNNNNNNGG 2 cut(s) 225, 585
BsmAI GTCTC 2 cut(s) 528, 821
BsmBI CGTCTC 1 cut(s) 821
BsmFI GGGAC 2 cut(s) 908, 922
BsnI GGCC 1 cut(s) 887
Bsp1286I GDGCHC 1 cut(s) 930
Bsp143I GATC 3 cut(s) 24, 588, 696
BspACI CCGC 1 cut(s) 222
BspANI GGCC 1 cut(s) 887
BspCNI CTCAG 1 cut(s) 761
BspHI TCATGA 1 cut(s) 780
BspLI GGNNCC 2 cut(s) 590, 610
BspMAI CTGCAG 1 cut(s) 259
BspMI ACCTGC 2 cut(s) 24, 139
BspPI GGATC 4 cut(s) 19, 583, 596, 704
BsrDI GCAATG 1 cut(s) 930
BsrI ACTGG 1 cut(s) 677
BssECI CCNNGG 2 cut(s) 557, 558
BssMI GATC 3 cut(s) 24, 588, 696
BssSI CACGAG 1 cut(s) 684
Bst2BI CACGAG 1 cut(s) 684
Bst2UI CCWGG 1 cut(s) 559
Bst4CI ACNGT 1 cut(s) 537
Bst6I CTCTTC 4 cut(s) 120, 306, 312, 318
BstC8I GCNNGC 1 cut(s) 141
BstDEI CTNAG 2 cut(s) 723, 748
BstF5I GGATG 2 cut(s) 719, 723
BstKTI GATC 3 cut(s) 27, 591, 699
BstMAI GTCTC 2 cut(s) 528, 821
BstMBI GATC 3 cut(s) 24, 588, 696
BstMWI GCNNNNNNNGC 4 cut(s) 145, 188, 497, 767
BstNI CCWGG 1 cut(s) 559
BstNSI RCATGY 1 cut(s) 100
BstSCI CCNGG 2 cut(s) 191, 557
BstSFI CTRYAG 2 cut(s) 255, 658
BstSLI GKGCMC 1 cut(s) 930
BstV1I GCAGC 2 cut(s) 269, 565
BstV2I GAAGAC 2 cut(s) 312, 425
BstX2I RGATCY 2 cut(s) 588, 696
BstXI CCANNNNNNTGG 1 cut(s) 605
BstYI RGATCY 2 cut(s) 588, 696
BsuRI GGCC 1 cut(s) 887
BtsCI GGATG 2 cut(s) 719, 723
BtsI GCAGTG 1 cut(s) 252
BtsIMutI CAGTG 2 cut(s) 252, 684
BveI ACCTGC 2 cut(s) 24, 139
Cac8I GCNNGC 1 cut(s) 141
CciI TCATGA 1 cut(s) 780
Cfr13I GGNCC 1 cut(s) 568
Csp6I GTAC 1 cut(s) 271
CspCI CAANNNNNGTGG 2 cut(s) 221, 256
CviAII CATG 9 cut(s) 97, 302, 333, 488, 580, 605, 628, 781, 936
CviQI GTAC 1 cut(s) 271
DdeI CTNAG 2 cut(s) 723, 748
DpnI GATC 3 cut(s) 26, 590, 698
DpnII GATC 3 cut(s) 24, 588, 696
Eam1104I CTCTTC 4 cut(s) 120, 306, 312, 318
EarI CTCTTC 4 cut(s) 120, 306, 312, 318
Eco147I AGGCCT 1 cut(s) 887
Eco47I GGWCC 1 cut(s) 568
Eco57I CTGAAG 1 cut(s) 192
EcoO109I RGGNCCY 1 cut(s) 568
EcoRII CCWGG 1 cut(s) 557
Esp3I CGTCTC 1 cut(s) 821
FaeI CATG 9 cut(s) 100, 305, 336, 491, 583, 608, 631, 784, 939
FalI AAGNNNNNCTT 4 cut(s) 171, 203, 197, 229
FaqI GGGAC 2 cut(s) 908, 922
FatI CATG 9 cut(s) 96, 301, 332, 487, 579, 604, 627, 780, 935
Fnu4HI GCNGC 2 cut(s) 258, 554
FokI GGATG 2 cut(s) 726, 730
Fsp4HI GCNGC 2 cut(s) 258, 554
FspBI CTAG 3 cut(s) 144, 762, 767
GluI GCNGC 2 cut(s) 258, 554
GsuI CTGGAG 1 cut(s) 423
HaeIII GGCC 1 cut(s) 887
HapII CCGG 1 cut(s) 193
Hin1II CATG 9 cut(s) 100, 305, 336, 491, 583, 608, 631, 784, 939
HincII GTYRAC 2 cut(s) 102, 291
HindII GTYRAC 2 cut(s) 102, 291
HinfI GANTC 1 cut(s) 784
HpaI GTTAAC 1 cut(s) 291
HpaII CCGG 1 cut(s) 193
Hpy166II GTNNAC 3 cut(s) 102, 273, 291
Hpy188I TCNGA 4 cut(s) 613, 646, 751, 794
Hpy188III TCNNGA 6 cut(s) 70, 440, 461, 686, 781, 859
Hpy8I GTNNAC 3 cut(s) 102, 273, 291
Hpy99I CGWCG 1 cut(s) 348
HpyCH4III ACNGT 1 cut(s) 537
HpyCH4V TGCA 6 cut(s) 36, 139, 257, 414, 530, 935
HpyF10VI GCNNNNNNNGC 4 cut(s) 145, 188, 497, 767
HpyF3I CTNAG 2 cut(s) 723, 748
Hsp92II CATG 9 cut(s) 100, 305, 336, 491, 583, 608, 631, 784, 939
KspAI GTTAAC 1 cut(s) 291
Kzo9I GATC 3 cut(s) 24, 588, 696
LmnI GCTCC 2 cut(s) 244, 614
Lsp1109I GCAGC 2 cut(s) 269, 565
LweI GCATC 1 cut(s) 23
MaeI CTAG 3 cut(s) 144, 762, 767
MaeIII GTNAC 3 cut(s) 77, 382, 545
MalI GATC 3 cut(s) 26, 590, 698
MboI GATC 3 cut(s) 24, 588, 696
MflI RGATCY 2 cut(s) 588, 696
MhlI GDGCHC 1 cut(s) 930
MluCI AATT 3 cut(s) 162, 295, 541
MmeI TCCRAC 1 cut(s) 72
MroXI GAANNNNTTC 1 cut(s) 788
MseI TTAA 3 cut(s) 153, 290, 564
MslI CAYNNNNRTG 1 cut(s) 902
MspI CCGG 1 cut(s) 193
MspR9I CCNGG 2 cut(s) 193, 559
MvaI CCWGG 1 cut(s) 559
MwoI GCNNNNNNNGC 4 cut(s) 145, 188, 497, 767
NciI CCSGG 1 cut(s) 193
NdeII GATC 3 cut(s) 24, 588, 696
NlaIII CATG 9 cut(s) 100, 305, 336, 491, 583, 608, 631, 784, 939
NlaIV GGNNCC 2 cut(s) 590, 610
NmuCI GTSAC 1 cut(s) 545
NspI RCATGY 1 cut(s) 100
PagI TCATGA 1 cut(s) 780
PasI CCCWGGG 1 cut(s) 558
PceI AGGCCT 1 cut(s) 887
PciI ACATGT 1 cut(s) 96
PdmI GAANNNNTTC 1 cut(s) 788
PfeI GAWTC 1 cut(s) 784
PflMI CCANNNNNTGG 1 cut(s) 585
PkrI GCNGC 2 cut(s) 259, 555
PpuMI RGGWCCY 1 cut(s) 568
PscI ACATGT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 568
Psp6I CCWGG 1 cut(s) 557
PspGI CCWGG 1 cut(s) 557
PspN4I GGNNCC 2 cut(s) 590, 610
PspPI GGNCC 1 cut(s) 568
PspPPI RGGWCCY 1 cut(s) 568
PsrI GAACNNNNNNTAC 2 cut(s) 22, 54
PstI CTGCAG 1 cut(s) 259
PsuI RGATCY 2 cut(s) 588, 696
RsaI GTAC 1 cut(s) 272
RsaNI GTAC 1 cut(s) 271
RseI CAYNNNNRTG 1 cut(s) 902
SaqAI TTAA 3 cut(s) 153, 290, 564
SatI GCNGC 2 cut(s) 258, 554
Sau3AI GATC 3 cut(s) 24, 588, 696
Sau96I GGNCC 1 cut(s) 568
ScrFI CCNGG 2 cut(s) 193, 559
SduI GDGCHC 1 cut(s) 930
SetI ASST 7 cut(s) 18, 145, 153, 184, 570, 641, 839
SfaNI GCATC 1 cut(s) 23
SfcI CTRYAG 2 cut(s) 255, 658
SinI GGWCC 1 cut(s) 568
SmiMI CAYNNNNRTG 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 376
SmoI CTYRAG 1 cut(s) 376
Sse9I AATT 3 cut(s) 162, 295, 541
SseBI AGGCCT 1 cut(s) 887
SsiI CCGC 1 cut(s) 222
SspMI CTAG 3 cut(s) 144, 762, 767
StuI AGGCCT 1 cut(s) 887
StyD4I CCNGG 2 cut(s) 191, 557
TaaI ACNGT 1 cut(s) 537
TasI AATT 3 cut(s) 162, 295, 541
TatI WGTACW 1 cut(s) 270
TfiI GAWTC 1 cut(s) 784
Tru1I TTAA 3 cut(s) 153, 290, 564
Tru9I TTAA 3 cut(s) 153, 290, 564
TscAI CASTG 2 cut(s) 259, 684
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 2 cut(s) 257, 553
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 8 cut(s) 26, 55, 123, 132, 218, 318, 683, 797
TspGWI ACGGA 2 cut(s) 17, 803
TspRI CASTG 2 cut(s) 259, 684
Van91I CCANNNNNTGG 1 cut(s) 585
VpaK11BI GGWCC 1 cut(s) 568
XapI RAATTY 1 cut(s) 162
XceI RCATGY 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 788
XspI CTAG 3 cut(s) 144, 762, 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.