Rmu_sc0004443.1_g000020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004443.1
OG0016677
Physical Location & Seq
Reverse (-)
70938 .. 72169
1232 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004443.1_g000020.1.cds

Sequence Viewer

Length: 582 bp
atgtttgggagagtaagagcctcgtcctctccggccgacagcttggagaggccgccttccaagattttcaaggacgatactctctccatctacgaggctacactgatgaagcttaaattgggttcaaaacgtaatctaagtctgtgctctaatgaggtaggggagatagatgccggttctaattcaagttctcctgactcggagccaatgaacatagaggtgaactgtgtttcctcgggaacttcaagctctcctgatttggttgccagctccccacaggaggaggttgtcacattagatacagattgttcttcagaaacaagttcaataagttcgagtgatagtaattctgcgggcacctcaaagcagccaaggagtacagaggtttctgttctctacctcttttctaaatttaggagagctcacgatgcaatcagctcgtcctcgggggaggcaatgacaattgatagtgttggtatttctcctagttctagtggttctcgatctgttaacaacacagaacaacagtcggaacaggaatgcatcagctctttacctgccactgacatacatatgctctaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

193

Amino Acids

20.35

Weight (kDa)

4.56

Isoelectric Point (pI)

76.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016677)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48490
fragaria_vesca FvH4_5g07590
malus_domestica MD06G1149100.v1.1 MD14G1163000.v1.1
prunus_persica Prupe.5G156300_v2.0.a1
rosa_chinensis RchiOBHm_Chr7g0190901
rosa_laevigata RLG00000004487
rosa_multiflora Rmu_sc0004443.1_g000020
rosa_samantha Rh1BG013800 Rh7AG114600 Rh7BG117000 Rh7CG120100 Rh7DG118400
rosa_wichuraiana Rw7G010000

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 565
AccB1I GGYRCC 1 cut(s) 356
AciI CCGC 2 cut(s) 53, 353
AcoI YGGCCR 1 cut(s) 33
AcsI RAATTY 1 cut(s) 410
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 1 cut(s) 379
AfiI CCNNNNNNNGG 1 cut(s) 280
AgsI TTSAA 5 cut(s) 70, 126, 186, 246, 327
AjuI GAANNNNNNNTTGG 2 cut(s) 101, 133
AloI GAACNNNNNNTCC 2 cut(s) 215, 247
AluBI AGCT 7 cut(s) 42, 112, 249, 270, 422, 438, 549
AluI AGCT 7 cut(s) 42, 112, 249, 270, 422, 438, 549
Alw21I GWGCWC 2 cut(s) 149, 424
Ama87I CYCGRG 2 cut(s) 235, 445
AoxI GGCC 2 cut(s) 33, 50
ApeKI GCWGC 1 cut(s) 367
ApoI RAATTY 1 cut(s) 410
AsuHPI GGTGA 1 cut(s) 232
AvaI CYCGRG 2 cut(s) 235, 445
BaeGI GKGCMC 1 cut(s) 359
BanI GGYRCC 1 cut(s) 356
BanII GRGCYC 1 cut(s) 424
Bbv12I GWGCWC 2 cut(s) 149, 424
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 95
BcgI CGANNNNNNTGC 2 cut(s) 420, 454
BfaI CTAG 2 cut(s) 486, 492
BfuAI ACCTGC 1 cut(s) 565
BisI GCNGC 2 cut(s) 53, 368
BlsI GCNGC 2 cut(s) 54, 369
BmeT110I CYCGRG 2 cut(s) 235, 445
BmiI GGNNCC 2 cut(s) 204, 358
BmsI GCATC 3 cut(s) 160, 418, 552
BsaJI CCNNGG 3 cut(s) 234, 371, 444
Bsc4I CCNNNNNNNGG 1 cut(s) 280
Bse118I RCCGGY 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 462
BseDI CCNNGG 3 cut(s) 234, 371, 444
BseLI CCNNNNNNNGG 1 cut(s) 280
BseMI GCAATG 1 cut(s) 462
BseRI GAGGAG 1 cut(s) 296
BseSI GKGCMC 1 cut(s) 359
BseX3I CGGCCG 1 cut(s) 33
BseXI GCAGC 1 cut(s) 379
Bsh1285I CGRYCG 1 cut(s) 36
BshFI GGCC 2 cut(s) 35, 52
BshNI GGYRCC 1 cut(s) 356
BsiEI CGRYCG 1 cut(s) 36
BsiHKAI GWGCWC 2 cut(s) 149, 424
BsiHKCI CYCGRG 2 cut(s) 235, 445
BsiSI CCGG 2 cut(s) 32, 174
BslI CCNNNNNNNGG 1 cut(s) 280
BsmI GAATGC 1 cut(s) 545
BsnI GGCC 2 cut(s) 35, 52
BsoBI CYCGRG 2 cut(s) 235, 445
Bsp1286I GDGCHC 3 cut(s) 149, 359, 424
Bsp143I GATC 1 cut(s) 503
BspACI CCGC 2 cut(s) 53, 353
BspANI GGCC 2 cut(s) 35, 52
BspLI GGNNCC 2 cut(s) 204, 358
BspMI ACCTGC 1 cut(s) 565
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 1 cut(s) 462
BsrFI RCCGGY 1 cut(s) 173
BssAI RCCGGY 1 cut(s) 173
BssECI CCNNGG 3 cut(s) 234, 371, 444
BssMI GATC 1 cut(s) 503
BssT1I CCWWGG 1 cut(s) 371
Bst4CI ACNGT 2 cut(s) 227, 528
BstC8I GCNNGC 2 cut(s) 268, 355
BstDEI CTNAG 1 cut(s) 137
BstKTI GATC 1 cut(s) 506
BstMBI GATC 1 cut(s) 503
BstMCI CGRYCG 1 cut(s) 36
BstMWI GCNNNNNNNGC 1 cut(s) 428
BstSLI GKGCMC 1 cut(s) 359
BstV1I GCAGC 1 cut(s) 379
BstZI CGGCCG 1 cut(s) 33
BsuRI GGCC 2 cut(s) 35, 52
BtsIMutI CAGTG 2 cut(s) 101, 561
BveI ACCTGC 1 cut(s) 565
Cac8I GCNNGC 2 cut(s) 268, 355
Cfr10I RCCGGY 1 cut(s) 173
Csp6I GTAC 1 cut(s) 378
CviQI GTAC 1 cut(s) 378
DdeI CTNAG 1 cut(s) 137
DpnI GATC 1 cut(s) 505
DpnII GATC 1 cut(s) 503
EaeI YGGCCR 1 cut(s) 33
EagI CGGCCG 1 cut(s) 33
Ecl136II GAGCTC 1 cut(s) 422
EclXI CGGCCG 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 371
Eco24I GRGCYC 1 cut(s) 424
Eco52I CGGCCG 1 cut(s) 33
Eco53kI GAGCTC 1 cut(s) 422
Eco57I CTGAAG 1 cut(s) 297
Eco88I CYCGRG 2 cut(s) 235, 445
EcoICRI GAGCTC 1 cut(s) 422
EcoT14I CCWWGG 1 cut(s) 371
EcoT22I ATGCAT 1 cut(s) 545
EcoT38I GRGCYC 1 cut(s) 424
ErhI CCWWGG 1 cut(s) 371
FaiI YATR 4 cut(s) 215, 569, 573, 575
FauI CCCGC 1 cut(s) 346
FauNDI CATATG 1 cut(s) 573
Fnu4HI GCNGC 2 cut(s) 53, 368
FriOI GRGCYC 1 cut(s) 424
Fsp4HI GCNGC 2 cut(s) 53, 368
FspBI CTAG 2 cut(s) 486, 492
GluI GCNGC 2 cut(s) 53, 368
HaeIII GGCC 2 cut(s) 35, 52
HapII CCGG 2 cut(s) 32, 174
HincII GTYRAC 1 cut(s) 511
HindII GTYRAC 1 cut(s) 511
HindIII AAGCTT 1 cut(s) 110
HinfI GANTC 1 cut(s) 197
HpaI GTTAAC 1 cut(s) 511
HpaII CCGG 2 cut(s) 32, 174
HphI GGTGA 1 cut(s) 232
Hpy166II GTNNAC 2 cut(s) 223, 511
Hpy188I TCNGA 3 cut(s) 202, 316, 532
Hpy188III TCNNGA 5 cut(s) 194, 237, 254, 425, 501
Hpy8I GTNNAC 2 cut(s) 223, 511
HpyAV CCTTC 1 cut(s) 66
HpyCH4III ACNGT 2 cut(s) 227, 528
HpyCH4IV ACGT 1 cut(s) 130
HpyCH4V TGCA 2 cut(s) 431, 543
HpyF10VI GCNNNNNNNGC 1 cut(s) 428
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 130
KspAI GTTAAC 1 cut(s) 511
Kzo9I GATC 1 cut(s) 503
LmnI GCTCC 2 cut(s) 202, 275
LpnPI CCDG 8 cut(s) 45, 187, 207, 263, 267, 280, 521, 570
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 3 cut(s) 160, 418, 552
MaeI CTAG 2 cut(s) 486, 492
MaeII ACGT 1 cut(s) 130
MaeIII GTNAC 1 cut(s) 289
MalI GATC 1 cut(s) 505
MboI GATC 1 cut(s) 503
MboII GAAGA 1 cut(s) 303
MfeI CAATTG 1 cut(s) 462
MhlI GDGCHC 3 cut(s) 149, 359, 424
MluCI AATT 5 cut(s) 116, 181, 346, 410, 462
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 1 cut(s) 510
Mph1103I ATGCAT 1 cut(s) 545
MseI TTAA 2 cut(s) 114, 510
MslI CAYNNNNRTG 2 cut(s) 218, 572
MspI CCGG 2 cut(s) 32, 174
MunI CAATTG 1 cut(s) 462
Mva1269I GAATGC 1 cut(s) 545
MwoI GCNNNNNNNGC 1 cut(s) 428
NdeI CATATG 1 cut(s) 573
NdeII GATC 1 cut(s) 503
NlaIV GGNNCC 2 cut(s) 204, 358
NmuCI GTSAC 1 cut(s) 289
NsiI ATGCAT 1 cut(s) 545
PctI GAATGC 1 cut(s) 545
PkrI GCNGC 2 cut(s) 54, 369
PleI GAGTC 1 cut(s) 191
PpsI GAGTC 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 424
PspN4I GGNNCC 2 cut(s) 204, 358
RsaI GTAC 1 cut(s) 379
RsaNI GTAC 1 cut(s) 378
RseI CAYNNNNRTG 2 cut(s) 218, 572
SacI GAGCTC 1 cut(s) 424
SaqAI TTAA 2 cut(s) 114, 510
SatI GCNGC 2 cut(s) 53, 368
Sau3AI GATC 1 cut(s) 503
SchI GAGTC 1 cut(s) 191
SduI GDGCHC 3 cut(s) 149, 359, 424
SfaNI GCATC 3 cut(s) 160, 418, 552
SmiMI CAYNNNNRTG 2 cut(s) 218, 572
Sse9I AATT 5 cut(s) 116, 181, 346, 410, 462
SsiI CCGC 2 cut(s) 53, 353
SspMI CTAG 2 cut(s) 486, 492
SstI GAGCTC 1 cut(s) 424
StyI CCWWGG 1 cut(s) 371
TaaI ACNGT 2 cut(s) 227, 528
TaiI ACGT 1 cut(s) 133
TaqI TCGA 2 cut(s) 335, 502
TasI AATT 5 cut(s) 116, 181, 346, 410, 462
TatI WGTACW 1 cut(s) 377
TauI GCSGC 1 cut(s) 55
Tru1I TTAA 2 cut(s) 114, 510
Tru9I TTAA 2 cut(s) 114, 510
TscAI CASTG 2 cut(s) 108, 568
TseFI GTSAC 1 cut(s) 289
TseI GCWGC 1 cut(s) 367
Tsp45I GTSAC 1 cut(s) 289
TspDTI ATGAA 2 cut(s) 122, 224
TspRI CASTG 2 cut(s) 108, 568
XapI RAATTY 1 cut(s) 410
XspI CTAG 2 cut(s) 486, 492
Zsp2I ATGCAT 1 cut(s) 545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.