Rmu_sc0004459.1_g000008

3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004459.1
OG0017709
Physical Location & Seq
Reverse (-)
30805 .. 35512
4708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004459.1_g000008.1.cds

Sequence Viewer

Length: 4194 bp
atgtcgttccatggctacgatcccggggatcgtaacaactggtatcagagcccgcaattgggcctatttccgacgagtccgacgggttacagtgcttggtattactgtcttccttcttctcatcctcacgttacccagtttccatcgcaaccaagctaccgaccacttccttctcaatttccattccaaacgagttgctcaccaccaccaccaccaccttcccacacaatcatcaatgggtttgttttccctcatcaatttccatcaacccatactcatcattcacctgattctaacccaaatccctggatctatccttctatttcacaaagccattcctatgacccatcgattaccagtgtcaattcacacttcgtctccaccaaaacaatgcctacatcacctgtttaccattctgctccttcaattccaccttttcagtcaccctcagtctccacttttgtgccccaacaccaatttactgttccagaacctcttcctctccattgcaagccgtttcggggttgcagatgtagagtcgtcatgggtgcaaaagcgagagtttccaaaaagacccgaaatccaaatctgctccatgatgtgggtaagtactcgaggtccaagaggtaccccaagcgcggtctctgggtcatcaaggccaaaaacggcgatatattcccacgccacaacgccaaggcgacagttgcgattatggaggagcttcatcagctacttgtgggtcaaacaactccgattactgagccggcctggagtccattgaagtttgggtcactacctgcgggttccgtcgctctcacagcggctaatggttcatcatcaatgttgtcggcgtcatcgagaggtatacggatttctgaaaaacccaacttaaattttagtccccatgaagttattttgggcttgaataagaattttattactggaattcatagtgagcacaccactacagcggtggtgactgaagaacttgtgttaaatgttgataaaaatattgctggaggtattcatggtgttaaattttctgatattgctgatggtgaagatgataatgaggataaggatgatgaagggggaaatggttgtgtgatatttgggagctctgcagcatcgaaagagttgcaacaagatattattgatggtaaatttaactctgaaagttttgacactttcaaagacggttgtcctcagatacatggccaatttgaatggtcggatgtagttagatctattaagaacctctatgaagtttccaaaactgtgaaggagaggaggtttagtgatggtgcccttcagcttgataactccaaagctgttattctgtttgatgaatatggcgatccatatgatagtttgttttccgaaaggaaggaatcaaattctcttgtggaggagtttatgctgttagcaaacagaacagcggctgaagtcatatctagagctttccctaacagtgcactactgcggaggcacccagagcctaatatgcgcaagcttaaagaatttgcagcattttgttccaagcatggtttagagttggatacttcttcttctggtcgtttccatcagtcactggagagaattagggaaaagctcaaggatgattctgttctgtttaatattctcatgaattatgctacaaaccctatgcagctggctatctacttttgtagtggagaattgaaacacagagaaaatgattggggtcactacggattggctgttcctctgtatactcatttcacattaccactacggaggtatcctgatattgtagtgcatcctgatattgtagtgcatcgcacactagctgctgctatagaggctgaggagttatatttggaacatcagagattgttgaataacctgaacaggggagacaaatttaagatgaggtgtttttctcttccactatctctcttgggtaatgattatttcaagtccacattacttatcacagctgtgatgggaggtaatccttatctatttgacgtggttgtactacatttcctttgccaaactaaagctagctgccacccaaagagctttattgttgatgaattttttgactttctgaaaagcaattcaaagttatcttttgtgaggtacatatgtgatgcacttctttcaagtgataattgtgtaggctttttgactaatatgcacaagttttttcaacacatggtgggacatgttcacaaagatgagtttttcatggcaacgtgctcttccacttcttgtcctccattacatgcacttgtgaagaaattgtttgctttatcacctgtggttgctatccattcattacaaccacttttggctgacatattgagagatgatgcacagcttatctttggaattatgatggttgtctctatgaatgagctgatgtttttgaggtctaagaaacaggggcttcaaattgggtatagtgatattgatgatggaaattattgtccttctatgagctgttctcttcacttgcctcatttcatgattacatggttttttgaattcacttctgtggcaattgactttggagatcttgcattacttcccccacaatacttttcagtacctattatattgttgtttagagttgtacactctcaggcaattgaatttgtgaaatcaagccatatactgcagggtcccaatagagaagccttgagaacccttgctacttctcatgttggtgttcatctatgtgcactactacatacttggcaagttacaaatgacgaaaatgaagctactgctgtggcatttgcactgatcaactatgttgttgcagtgtgtattcagctcagctcacgcaaggctgcctttgatacagatatatatgttgcaagtgcgcttattgacatgtattcaaagtgtggtctgtctctcaattttcaagagaaggaagctttcacttactttgagaagatatggcaatatgagatgtttcctacgccattctcttatgctaatttatggagttgttgtgcaaagctagcttctgcattccaagagaaacagattactacattgacattaatgaaaatttcacttgccaaagtgtttgaaattgaaagtgatggtgggttgtcacacgatttggagatgatgtgtttgcttattactggacatgagtgggctgctgatgtgatatcttcagatgttcctcttcctagtgtggatgacctcgcagaccagcttgttgaagtgcttgatttatttggtataattttaactacaccaacttaccagttgtcgacgactccttttgttacggacgaatggttggttgaagcagatatgatcattaaagcaaggcaagagaggagaatagtttttgctgaaaccaaagaagagtccttcaagttcattcctcttcgtaagcacgaaagcccatctgtgaatccctttaaaagccttaataattccgtccctgatggatctaagttgaagattattcaatcctgcaatcgcctctttctctgcctttgcaagtcgacgaaagaaagaagacatgatctcgtatacgttttcaatcccacaaccaaccaattccgggctgtttcttctccaaggtttggggaagacatgggcgaatttgcttttgtacgttacgaatttggttttgtacgttatgctttggcttttgatccttccaaatcgcctcattacaaggtggtctgcgtgactaacttcaatgatcattatgatggggggcaccacgagatagacatatattcatcagagactggaaagtggaagcatctcgatactcctttcttccccagcccctctgacgagggcgcaaactatgattgcaaggagatggccacgcactttgactacaggagcaggaaaggcaccgtatactgcaatggcgcagttcattggataagaggcagtcgtgaagcaagattgccattctgcacatttggggatggtagaaatgagttcataagaaacgaaagtgatgtgctgcactactttgacgtaggcgaagaacgtttgctgctagcctccgctacccctggctgtccccttggttgtcaaaaaaattccattaatttcctccctagccctcattattcctttaagtctccctctgagtatcccagattggctcgtagatattttggggagtgctgtggccgtttgtacttgattgagacttaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000070 GO:0000175 GO:0000178 GO:0000278 GO:0000280 GO:0000287 GO:0000291 GO:0000819 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004527 GO:0004532 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005844 GO:0006139 GO:0006364 GO:0006396 GO:0006401 GO:0006402 GO:0006725 GO:0006807 GO:0006996 GO:0007049 GO:0007059 GO:0008150 GO:0008152 GO:0008187 GO:0008266 GO:0008285 GO:0008408 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010467 GO:0010468 GO:0010586 GO:0010587 GO:0010605 GO:0010629 GO:0016043 GO:0016070 GO:0016071 GO:0016072 GO:0016787 GO:0016788 GO:0016796 GO:0016896 GO:0019222 GO:0019439 GO:0019827 GO:0022402 GO:0022613 GO:0032501 GO:0032502 GO:0032991 GO:0034470 GO:0034641 GO:0034655 GO:0034660 GO:0034661 GO:0035770 GO:0036464 GO:0042127 GO:0042254 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0043632 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046700 GO:0046872 GO:0048285 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0051276 GO:0051304 GO:0051306 GO:0060255 GO:0065007 GO:0071704 GO:0071840 GO:0090304 GO:0090305 GO:0090501 GO:0090503 GO:0097159 GO:0098727 GO:0098813 GO:0140014 GO:0140098 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1902494 GO:1903047 GO:1905354 GO:1990074 GO:1990904
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RNB

Protein Analysis

1397

Amino Acids

157.6

Weight (kDa)

6.39

Isoelectric Point (pI)

44.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1508
Acc36I ACCTGC 1 cut(s) 805
Acc65I GGTACC 1 cut(s) 627
AccB1I GGYRCC 5 cut(s) 627, 1304, 1488, 3738, 3881
AccB7I CCANNNNNTGG 2 cut(s) 601, 3599
AccI GTMKAC 6 cut(s) 865, 1751, 3278, 3518, 3546, 3888
AccII CGCG 1 cut(s) 639
AciI CCGC 8 cut(s) 53, 639, 800, 821, 971, 1439, 1483, 4041
AclI AACGTT 1 cut(s) 4024
AclWI GGATC 6 cut(s) 14, 36, 317, 1352, 3469, 3665
AcoI YGGCCR 3 cut(s) 1216, 3849, 4168
AcuI CTGAAG 4 cut(s) 1002, 1295, 1464, 3162
AcyI GRCGYC 1 cut(s) 851
AdeI CACNNNGTG 1 cut(s) 2203
AfaI GTAC 9 cut(s) 611, 629, 2019, 2126, 2595, 2622, 3630, 3651, 4178
AfiI CCNNNNNNNGG 8 cut(s) 58, 59, 521, 601, 638, 1891, 3577, 3599
AflIII ACRYGT 2 cut(s) 2209, 2883
AjiI CACGTC 1 cut(s) 2011
AjnI CCWGG 3 cut(s) 305, 767, 4048
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AleI CACNNNNGTG 3 cut(s) 461, 1979, 2540
AloI GAACNNNNNNTCC 2 cut(s) 1725, 1757
Alw21I GWGCWC 5 cut(s) 960, 1121, 1477, 2246, 2731
Alw26I GTCTC 9 cut(s) 382, 457, 647, 1890, 2395, 2910, 3761, 4122, 4181
Alw44I GTGCAC 2 cut(s) 1473, 2727
AlwI GGATC 6 cut(s) 14, 36, 317, 1352, 3469, 3665
AlwNI CAGNNNCTG 3 cut(s) 1442, 1591, 3770
Ama87I CYCGRG 2 cut(s) 23, 613
AoxI GGCC 6 cut(s) 61, 657, 765, 1216, 3849, 4168
ApaLI GTGCAC 2 cut(s) 1473, 2727
AseI ATTAAT 2 cut(s) 3059, 4083
Asp700I GAANNNNTTC 1 cut(s) 495
Asp718I GGTACC 1 cut(s) 627
AspLEI GCGC 5 cut(s) 639, 1509, 2875, 3827, 3902
AspS9I GGNCC 3 cut(s) 61, 618, 2669
AsuC2I CCSGG 3 cut(s) 24, 25, 3578
AsuHPI GGTGA 7 cut(s) 192, 276, 393, 435, 988, 1070, 2292
AsuNHI GCTAGC 3 cut(s) 2045, 3016, 4033
AvaI CYCGRG 2 cut(s) 23, 613
AvaII GGWCC 2 cut(s) 618, 2669
BaeGI GKGCMC 5 cut(s) 468, 1309, 1477, 2731, 3741
BaeI ACNNNNGTAYC 4 cut(s) 1763, 1796, 2116, 2149
BalI TGGCCA 2 cut(s) 1218, 3851
BanI GGYRCC 5 cut(s) 627, 1304, 1488, 3738, 3881
BanII GRGCYC 2 cut(s) 53, 1121
BauI CACGAG 1 cut(s) 3743
BbsI GAAGAC 3 cut(s) 101, 3538, 3612
Bbv12I GWGCWC 5 cut(s) 960, 1121, 1477, 2246, 2731
BbvCI CCTCAGC 1 cut(s) 1845
BceAI ACGGC 3 cut(s) 499, 682, 4155
BcgI CGANNNNNNTGC 2 cut(s) 1120, 1154
BciT130I CCWGG 3 cut(s) 307, 769, 4050
BciVI GTATCC 3 cut(s) 1552, 1791, 4140
BclI TGATCA 3 cut(s) 2793, 3324, 3721
BcnI CCSGG 3 cut(s) 24, 25, 3578
BcoDI GTCTC 9 cut(s) 382, 457, 647, 1890, 2395, 2910, 3761, 4122, 4181
BfaI CTAG 7 cut(s) 1455, 1826, 2046, 3017, 3195, 4034, 4095
BfmI CTRYAG 5 cut(s) 966, 1122, 1836, 2663, 3865
BfuAI ACCTGC 1 cut(s) 805
BfuI GTATCC 3 cut(s) 1552, 1791, 4140
BglII AGATCT 2 cut(s) 1244, 2560
BlpI GCTNAGC 1 cut(s) 2825
BmcAI AGTACT 1 cut(s) 611
Bme1390I CCNGG 6 cut(s) 24, 25, 307, 769, 3578, 4050
Bme18I GGWCC 2 cut(s) 618, 2669
BmeT110I CYCGRG 2 cut(s) 23, 613
BmgBI CACGTC 1 cut(s) 2011
BmgT120I GGNCC 3 cut(s) 61, 618, 2669
BmiI GGNNCC 8 cut(s) 629, 805, 1306, 1490, 2670, 2671, 3740, 3883
BmrFI CCNGG 6 cut(s) 24, 25, 307, 769, 3578, 4050
BmrI ACTGGG 1 cut(s) 130
BmsI GCATC 6 cut(s) 1136, 1807, 1825, 2125, 2347, 3793
BmtI GCTAGC 3 cut(s) 2049, 3020, 4037
BmuI ACTGGG 1 cut(s) 130
BoxI GACNNNNGTC 1 cut(s) 1200
BpiI GAAGAC 3 cut(s) 101, 3538, 3612
BplI GAGNNNNNCTC 2 cut(s) 2476, 2508
BpmI CTGGAG 3 cut(s) 790, 1038, 1613
Bpu10I CCTNAGC 1 cut(s) 1845
Bpu1102I GCTNAGC 1 cut(s) 2825
BpuEI CTTGAG 2 cut(s) 1598, 2707
BpuMI CCSGG 3 cut(s) 24, 25, 3578
Bsa29I ATCGAT 1 cut(s) 350
BsaHI GRCGYC 1 cut(s) 851
BsaI GGTCTC 1 cut(s) 647
BsaJI CCNNGG 8 cut(s) 10, 23, 24, 305, 693, 3593, 4048, 4060
Bsc4I CCNNNNNNNGG 8 cut(s) 58, 59, 521, 601, 638, 1891, 3577, 3599
Bse118I RCCGGY 1 cut(s) 763
Bse1I ACTGG 8 cut(s) 44, 136, 357, 946, 1596, 3151, 3271, 3775
Bse3DI GCAATG 2 cut(s) 505, 3901
BseBI CCWGG 3 cut(s) 307, 769, 4050
BseCI ATCGAT 1 cut(s) 350
BseDI CCNNGG 8 cut(s) 10, 23, 24, 305, 693, 3593, 4048, 4060
BseGI GGATG 7 cut(s) 121, 1087, 1240, 1624, 1798, 3208, 3964
BseLI CCNNNNNNNGG 8 cut(s) 58, 59, 521, 601, 638, 1891, 3577, 3599
BseMI GCAATG 2 cut(s) 505, 3901
BseMII CTCAG 7 cut(s) 462, 750, 1220, 1836, 2642, 2839, 4116
BseNI ACTGG 8 cut(s) 44, 136, 357, 946, 1596, 3151, 3271, 3775
BseRI GAGGAG 5 cut(s) 731, 1303, 1424, 1862, 3361
BseSI GKGCMC 5 cut(s) 468, 1309, 1477, 2731, 3741
BseYI CCCAGC 1 cut(s) 3806
BsgI GTGCAG 2 cut(s) 3931, 3983
Bsh1236I CGCG 1 cut(s) 639
BshFI GGCC 6 cut(s) 63, 659, 767, 1218, 3851, 4170
BshNI GGYRCC 5 cut(s) 627, 1304, 1488, 3738, 3881
BshVI ATCGAT 1 cut(s) 350
BsiHKAI GWGCWC 5 cut(s) 960, 1121, 1477, 2246, 2731
BsiHKCI CYCGRG 2 cut(s) 23, 613
BsiSI CCGG 3 cut(s) 24, 764, 3577
BslFI GGGAC 5 cut(s) 885, 2220, 2655, 3437, 4041
BslI CCNNNNNNNGG 8 cut(s) 58, 59, 521, 601, 638, 1891, 3577, 3599
BsmAI GTCTC 9 cut(s) 382, 457, 647, 1890, 2395, 2910, 3761, 4122, 4181
BsmBI CGTCTC 1 cut(s) 382
BsmFI GGGAC 5 cut(s) 885, 2220, 2655, 3437, 4041
BsmI GAATGC 1 cut(s) 3026
BsnI GGCC 6 cut(s) 63, 659, 767, 1218, 3851, 4170
Bso31I GGTCTC 1 cut(s) 647
BsoBI CYCGRG 2 cut(s) 23, 613
Bsp1286I GDGCHC 9 cut(s) 53, 468, 960, 1121, 1309, 1477, 2246, 2731, 3741
Bsp1407I TGTACA 1 cut(s) 2620
Bsp1720I GCTNAGC 1 cut(s) 2825
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 8 cut(s) 53, 639, 800, 821, 971, 1439, 1483, 4041
BspANI GGCC 6 cut(s) 63, 659, 767, 1218, 3851, 4170
BspCNI CTCAG 7 cut(s) 461, 751, 1219, 1837, 2641, 2838, 4117
BspDI ATCGAT 1 cut(s) 350
BspFNI CGCG 1 cut(s) 639
BspHI TCATGA 2 cut(s) 1644, 2511
BspLI GGNNCC 8 cut(s) 629, 805, 1306, 1490, 2670, 2671, 3740, 3883
BspMAI CTGCAG 2 cut(s) 1126, 2667
BspMI ACCTGC 1 cut(s) 805
BspOI GCTAGC 3 cut(s) 2049, 3020, 4037
BspPI GGATC 6 cut(s) 14, 36, 317, 1352, 3469, 3665
BspQI GCTCTTC 1 cut(s) 2251
BspT107I GGYRCC 5 cut(s) 627, 1304, 1488, 3738, 3881
BspTNI GGTCTC 1 cut(s) 647
BsrDI GCAATG 2 cut(s) 505, 3901
BsrFI RCCGGY 1 cut(s) 763
BsrGI TGTACA 1 cut(s) 2620
BsrI ACTGG 8 cut(s) 44, 136, 357, 946, 1596, 3151, 3271, 3775
BssAI RCCGGY 1 cut(s) 763
BssECI CCNNGG 8 cut(s) 10, 23, 24, 305, 693, 3593, 4048, 4060
BssNAI GTATAC 4 cut(s) 866, 1752, 3547, 3889
BssNI GRCGYC 1 cut(s) 851
BssSI CACGAG 1 cut(s) 3743
BssT1I CCWWGG 4 cut(s) 10, 693, 3593, 4060
Bst1107I GTATAC 4 cut(s) 866, 1752, 3547, 3889
Bst2BI CACGAG 1 cut(s) 3743
Bst2UI CCWGG 3 cut(s) 307, 769, 4050
Bst4CI ACNGT 8 cut(s) 92, 107, 484, 703, 1199, 1279, 1472, 3886
Bst6I CTCTTC 7 cut(s) 501, 1929, 2251, 2499, 3195, 3369, 3402
BstACI GRCGYC 1 cut(s) 851
BstAUI TGTACA 1 cut(s) 2620
BstC8I GCNNGC 8 cut(s) 53, 512, 765, 1511, 1674, 2047, 3018, 4035
BstDEI CTNAG 9 cut(s) 448, 759, 1206, 1845, 2421, 2628, 2825, 3465, 4125
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 7 cut(s) 121, 1087, 1240, 1624, 1798, 3208, 3964
BstFNI CGCG 1 cut(s) 639
BstHHI GCGC 5 cut(s) 639, 1509, 2875, 3827, 3902
BstMAI GTCTC 9 cut(s) 382, 457, 647, 1890, 2395, 2910, 3761, 4122, 4181
BstMWI GCNNNNNNNGC 8 cut(s) 704, 727, 818, 1495, 1504, 1841, 3017, 3879
BstNI CCWGG 3 cut(s) 307, 769, 4050
BstNSI RCATGY 3 cut(s) 2213, 2273, 2887
BstPAI GACNNNNGTC 1 cut(s) 1200
BstSCI CCNGG 6 cut(s) 22, 23, 305, 767, 3576, 4048
BstSFI CTRYAG 5 cut(s) 966, 1122, 1836, 2663, 3865
BstSLI GKGCMC 5 cut(s) 468, 1309, 1477, 2731, 3741
BstUI CGCG 1 cut(s) 639
BstV2I GAAGAC 3 cut(s) 101, 3538, 3612
BstX2I RGATCY 4 cut(s) 309, 1244, 2560, 3461
BstXI CCANNNNNNTGG 1 cut(s) 306
BstYI RGATCY 4 cut(s) 309, 1244, 2560, 3461
BstZ17I GTATAC 4 cut(s) 866, 1752, 3547, 3889
Bsu15I ATCGAT 1 cut(s) 350
BsuI GTATCC 3 cut(s) 1552, 1791, 4140
BsuRI GGCC 6 cut(s) 63, 659, 767, 1218, 3851, 4170
BsuTUI ATCGAT 1 cut(s) 350
BtgI CCRYGG 1 cut(s) 10
BtgZI GCGATG 2 cut(s) 129, 1802
BtrI CACGTC 1 cut(s) 2011
BtsCI GGATG 7 cut(s) 121, 1087, 1240, 1624, 1798, 3208, 3964
BtsI GCAGTG 1 cut(s) 2817
BtsIMutI CAGTG 6 cut(s) 97, 364, 1477, 1589, 2789, 2817
BveI ACCTGC 1 cut(s) 805
Cac8I GCNNGC 8 cut(s) 53, 512, 765, 1511, 1674, 2047, 3018, 4035
CaiI CAGNNNCTG 3 cut(s) 1442, 1591, 3770
CciI TCATGA 2 cut(s) 1644, 2511
CfoI GCGC 5 cut(s) 639, 1509, 2875, 3827, 3902
Cfr10I RCCGGY 1 cut(s) 763
Cfr13I GGNCC 3 cut(s) 61, 618, 2669
Cfr9I CCCGGG 1 cut(s) 23
ClaI ATCGAT 1 cut(s) 350
CseI GACGC 1 cut(s) 840
Csp6I GTAC 9 cut(s) 610, 628, 2018, 2125, 2594, 2621, 3629, 3650, 4177
CspCI CAANNNNNGTGG 2 cut(s) 3841, 3876
CviQI GTAC 9 cut(s) 610, 628, 2018, 2125, 2594, 2621, 3629, 3650, 4177
DdeI CTNAG 9 cut(s) 448, 759, 1206, 1845, 2421, 2628, 2825, 3465, 4125
DraI TTTAAA 1 cut(s) 3433
DraIII CACNNNGTG 1 cut(s) 2203
EaeI YGGCCR 3 cut(s) 1216, 3849, 4168
Eam1104I CTCTTC 7 cut(s) 501, 1929, 2251, 2499, 3195, 3369, 3402
EarI CTCTTC 7 cut(s) 501, 1929, 2251, 2499, 3195, 3369, 3402
Ecl136II GAGCTC 1 cut(s) 1119
Eco130I CCWWGG 4 cut(s) 10, 693, 3593, 4060
Eco24I GRGCYC 2 cut(s) 53, 1121
Eco31I GGTCTC 1 cut(s) 647
Eco32I GATATC 1 cut(s) 3174
Eco47I GGWCC 2 cut(s) 618, 2669
Eco53kI GAGCTC 1 cut(s) 1119
Eco57I CTGAAG 4 cut(s) 1002, 1295, 1464, 3162
Eco88I CYCGRG 2 cut(s) 23, 613
EcoICRI GAGCTC 1 cut(s) 1119
EcoO109I RGGNCCY 1 cut(s) 2669
EcoRI GAATTC 2 cut(s) 945, 2531
EcoRII CCWGG 3 cut(s) 305, 767, 4048
EcoRV GATATC 1 cut(s) 3174
EcoT14I CCWWGG 4 cut(s) 10, 693, 3593, 4060
EcoT38I GRGCYC 2 cut(s) 53, 1121
ErhI CCWWGG 4 cut(s) 10, 693, 3593, 4060
Esp3I CGTCTC 1 cut(s) 382
FalI AAGNNNNNCTT 2 cut(s) 2828, 2860
FaqI GGGAC 5 cut(s) 885, 2220, 2655, 3437, 4041
FauI CCCGC 2 cut(s) 60, 793
FauNDI CATATG 2 cut(s) 1363, 2129
FbaI TGATCA 3 cut(s) 2793, 3324, 3721
FblI GTMKAC 6 cut(s) 865, 1751, 3278, 3518, 3546, 3888
FokI GGATG 7 cut(s) 108, 1094, 1247, 1631, 1785, 3215, 3971
FriOI GRGCYC 2 cut(s) 53, 1121
FspBI CTAG 7 cut(s) 1455, 1826, 2046, 3017, 3195, 4034, 4095
FspI TGCGCA 1 cut(s) 1508
GlaI GCGC 5 cut(s) 638, 1508, 2874, 3826, 3901
GsaI CCCAGC 1 cut(s) 3810
GsuI CTGGAG 3 cut(s) 790, 1038, 1613
HaeIII GGCC 6 cut(s) 63, 659, 767, 1218, 3851, 4170
HapII CCGG 3 cut(s) 24, 764, 3577
HgaI GACGC 1 cut(s) 840
HhaI GCGC 5 cut(s) 639, 1509, 2875, 3827, 3902
Hin1I GRCGYC 1 cut(s) 851
Hin6I GCGC 5 cut(s) 637, 1507, 2873, 3825, 3900
HinP1I GCGC 5 cut(s) 637, 1507, 2873, 3825, 3900
HincII GTYRAC 2 cut(s) 3279, 3519
HindII GTYRAC 2 cut(s) 3279, 3519
HindIII AAGCTT 2 cut(s) 1511, 2928
HinfI GANTC 9 cut(s) 76, 290, 537, 772, 1391, 1622, 3283, 3377, 3424
HpaII CCGG 3 cut(s) 24, 764, 3577
HphI GGTGA 7 cut(s) 192, 276, 393, 435, 988, 1070, 2292
Hpy99I CGWCG 5 cut(s) 76, 85, 812, 3283, 3523
HpyCH4III ACNGT 8 cut(s) 92, 107, 484, 703, 1199, 1279, 1472, 3886
HpyCH4IV ACGT 8 cut(s) 129, 2010, 2240, 3549, 3631, 3652, 4011, 4024
HpyF10VI GCNNNNNNNGC 8 cut(s) 704, 727, 818, 1495, 1504, 1841, 3017, 3879
HpyF3I CTNAG 9 cut(s) 448, 759, 1206, 1845, 2421, 2628, 2825, 3465, 4125
HpySE526I ACGT 8 cut(s) 129, 2010, 2240, 3549, 3631, 3652, 4011, 4024
Hsp92I GRCGYC 1 cut(s) 851
HspAI GCGC 5 cut(s) 637, 1507, 2873, 3825, 3900
KflI GGGWCCC 1 cut(s) 2669
KpnI GGTACC 1 cut(s) 631
KroI GCCGGC 1 cut(s) 763
KroNI GCCGGC 1 cut(s) 765
Ksp22I TGATCA 3 cut(s) 2793, 3324, 3721
LguI GCTCTTC 1 cut(s) 2251
LmnI GCTCC 5 cut(s) 424, 597, 718, 1116, 3870
LweI GCATC 6 cut(s) 1136, 1807, 1825, 2125, 2347, 3793
MaeI CTAG 7 cut(s) 1455, 1826, 2046, 3017, 3195, 4034, 4095
MaeII ACGT 8 cut(s) 129, 2010, 2240, 3549, 3631, 3652, 4011, 4024
MfeI CAATTG 3 cut(s) 56, 2547, 2634
MflI RGATCY 4 cut(s) 309, 1244, 2560, 3461
MhlI GDGCHC 9 cut(s) 53, 468, 960, 1121, 1309, 1477, 2246, 2731, 3741
MlsI TGGCCA 2 cut(s) 1218, 3851
MluNI TGGCCA 2 cut(s) 1218, 3851
MlyI GAGTC 5 cut(s) 85, 546, 781, 3277, 3386
MmeI TCCRAC 4 cut(s) 95, 104, 1212, 1536
Mox20I TGGCCA 2 cut(s) 1218, 3851
MroNI GCCGGC 1 cut(s) 763
MroXI GAANNNNTTC 1 cut(s) 495
MscI TGGCCA 2 cut(s) 1218, 3851
MslI CAYNNNNRTG 8 cut(s) 339, 461, 1979, 2220, 2540, 2712, 2724, 3729
Msp20I TGGCCA 2 cut(s) 1218, 3851
MspA1I CMGCKG 5 cut(s) 821, 971, 1439, 1672, 1979
MspI CCGG 3 cut(s) 24, 764, 3577
MspR9I CCNGG 6 cut(s) 24, 25, 307, 769, 3578, 4050
MunI CAATTG 3 cut(s) 56, 2547, 2634
Mva1269I GAATGC 1 cut(s) 3026
MvaI CCWGG 3 cut(s) 307, 769, 4050
MvnI CGCG 1 cut(s) 639
MwoI GCNNNNNNNGC 8 cut(s) 704, 727, 818, 1495, 1504, 1841, 3017, 3879
NaeI GCCGGC 1 cut(s) 765
NciI CCSGG 3 cut(s) 24, 25, 3578
NcoI CCATGG 1 cut(s) 10
NdeI CATATG 2 cut(s) 1363, 2129
NgoMIV GCCGGC 1 cut(s) 763
NheI GCTAGC 3 cut(s) 2045, 3016, 4033
NlaIV GGNNCC 8 cut(s) 629, 805, 1306, 1490, 2670, 2671, 3740, 3883
NmuCI GTSAC 7 cut(s) 441, 789, 976, 1587, 1724, 3111, 3706
NsbI TGCGCA 1 cut(s) 1508
NspI RCATGY 3 cut(s) 2213, 2273, 2887
OliI CACNNNNGTG 3 cut(s) 461, 1979, 2540
PaeR7I CTCGAG 1 cut(s) 613
PagI TCATGA 2 cut(s) 1644, 2511
PciI ACATGT 2 cut(s) 2209, 2883
PciSI GCTCTTC 1 cut(s) 2251
PcsI WCGNNNNNNNCGW 1 cut(s) 854
PctI GAATGC 1 cut(s) 3026
PdiI GCCGGC 1 cut(s) 765
PdmI GAANNNNTTC 1 cut(s) 495
PfeI GAWTC 4 cut(s) 290, 1391, 1622, 3424
PflMI CCANNNNNTGG 2 cut(s) 601, 3599
PleI GAGTC 5 cut(s) 84, 545, 780, 3277, 3385
PpsI GAGTC 5 cut(s) 84, 545, 780, 3277, 3385
PpuMI RGGWCCY 1 cut(s) 2669
PscI ACATGT 2 cut(s) 2209, 2883
PshAI GACNNNNGTC 1 cut(s) 1200
PshBI ATTAAT 2 cut(s) 3059, 4083
Psp124BI GAGCTC 1 cut(s) 1121
Psp1406I AACGTT 1 cut(s) 4024
Psp5II RGGWCCY 1 cut(s) 2669
Psp6I CCWGG 3 cut(s) 305, 767, 4048
PspFI CCCAGC 1 cut(s) 3806
PspGI CCWGG 3 cut(s) 305, 767, 4048
PspN4I GGNNCC 8 cut(s) 629, 805, 1306, 1490, 2670, 2671, 3740, 3883
PspPI GGNCC 3 cut(s) 61, 618, 2669
PspPPI RGGWCCY 1 cut(s) 2669
PspXI VCTCGAGB 1 cut(s) 613
PsrI GAACNNNNNNTAC 2 cut(s) 2683, 2715
PstI CTGCAG 2 cut(s) 1126, 2667
PstNI CAGNNNCTG 3 cut(s) 1442, 1591, 3770
PsuI RGATCY 4 cut(s) 309, 1244, 2560, 3461
PvuII CAGCTG 2 cut(s) 1672, 1979
RsaI GTAC 9 cut(s) 611, 629, 2019, 2126, 2595, 2622, 3630, 3651, 4178
RsaNI GTAC 9 cut(s) 610, 628, 2018, 2125, 2594, 2621, 3629, 3650, 4177
RseI CAYNNNNRTG 8 cut(s) 339, 461, 1979, 2220, 2540, 2712, 2724, 3729
SacI GAGCTC 1 cut(s) 1121
SalI GTCGAC 2 cut(s) 3277, 3517
SapI GCTCTTC 1 cut(s) 2251
Sau96I GGNCC 3 cut(s) 61, 618, 2669
ScaI AGTACT 1 cut(s) 611
SchI GAGTC 5 cut(s) 85, 546, 781, 3277, 3386
ScrFI CCNGG 6 cut(s) 24, 25, 307, 769, 3578, 4050
SduI GDGCHC 9 cut(s) 53, 468, 960, 1121, 1309, 1477, 2246, 2731, 3741
SfaNI GCATC 6 cut(s) 1136, 1807, 1825, 2125, 2347, 3793
SfcI CTRYAG 5 cut(s) 966, 1122, 1836, 2663, 3865
Sfr274I CTCGAG 1 cut(s) 613
SinI GGWCC 2 cut(s) 618, 2669
SlaI CTCGAG 1 cut(s) 613
SmaI CCCGGG 1 cut(s) 25
SmiMI CAYNNNNRTG 8 cut(s) 339, 461, 1979, 2220, 2540, 2712, 2724, 3729
SmlI CTYRAG 3 cut(s) 613, 1613, 2686
SmoI CTYRAG 3 cut(s) 613, 1613, 2686
SsiI CCGC 8 cut(s) 53, 639, 800, 821, 971, 1439, 1483, 4041
SspI AATATT 2 cut(s) 1012, 1639
SspMI CTAG 7 cut(s) 1455, 1826, 2046, 3017, 3195, 4034, 4095
SstI GAGCTC 1 cut(s) 1121
StyD4I CCNGG 6 cut(s) 22, 23, 305, 767, 3576, 4048
StyI CCWWGG 4 cut(s) 10, 693, 3593, 4060
TaaI ACNGT 8 cut(s) 92, 107, 484, 703, 1199, 1279, 1472, 3886
TaiI ACGT 8 cut(s) 132, 2013, 2243, 3552, 3634, 3655, 4014, 4027
TaqI TCGA 7 cut(s) 350, 614, 857, 1130, 3278, 3518, 3789
TatI WGTACW 4 cut(s) 609, 2017, 2620, 4176
TauI GCSGC 2 cut(s) 824, 1442
TfiI GAWTC 4 cut(s) 290, 1391, 1622, 3424
TscAI CASTG 6 cut(s) 97, 364, 1477, 1596, 2796, 2817
TseFI GTSAC 7 cut(s) 441, 789, 976, 1587, 1724, 3111, 3706
Tsp45I GTSAC 7 cut(s) 441, 789, 976, 1587, 1724, 3111, 3706
TspGWI ACGGA 6 cut(s) 796, 883, 1746, 1789, 3311, 3439
TspMI CCCGGG 1 cut(s) 23
TspRI CASTG 6 cut(s) 97, 364, 1477, 1596, 2796, 2817
Van91I CCANNNNNTGG 2 cut(s) 601, 3599
VneI GTGCAC 2 cut(s) 1473, 2727
VpaK11BI GGWCC 2 cut(s) 618, 2669
VspI ATTAAT 2 cut(s) 3059, 4083
XbaI TCTAGA 1 cut(s) 1454
XceI RCATGY 3 cut(s) 2213, 2273, 2887
XcmI CCANNNNNNNNNTGG 1 cut(s) 970
XhoI CTCGAG 1 cut(s) 613
XmaI CCCGGG 1 cut(s) 23
XmiI GTMKAC 6 cut(s) 865, 1751, 3278, 3518, 3546, 3888
XmnI GAANNNNTTC 1 cut(s) 495
XspI CTAG 7 cut(s) 1455, 1826, 2046, 3017, 3195, 4034, 4095
ZrmI AGTACT 1 cut(s) 611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.