Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000001

Orthogroup ID

OG0017709

Physical Location & Seq

Genomic Coordinates Reverse (-)

88363139 .. 88367613

Gene Structure 4475 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_1G00066910.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 4080 bp

ATGGATTCTGATCCTAACATTTGGTATCAGAGCCCTTCGATCCATATGGGCGAGGTGGTTCTTCCGGTAGTGCAAAACTGCATCAGTTCGATGGCTGTTTTTCATCAAACTATGAACGATCTTCATAACAACTTCATGGCAGAGATTCGTCGGATTCAGGAATACCGCTCAAAATTTCCAACTGTTGCAACCCCTACATACAGTGGTCCATGGTCTGCTCCAGAAGATCCTACACTTCCAAGTCCTTGGAAGGGTTTAATCGATGGTTCATCAGGTTCATTGTATTACTGGAATCCTGAAACTAACCATACCCAGTATGAAAGGCCATCTCCACCTACTCCTTTGTATCCACCAACATTCACCTCCTCAGATTACTCCATTCACTCAAACCCTCAACCATACACCTCCGTTTACCACCACAGTATCACTCCTCCAATTACCCACCCTACAACTCCCCACCCTTATCGTGTTGCTTCGATTCACCCCAACCCTCAACACCAATCACACAACCTACCAATCAATTACCCTCACTCTGAACCTATTGAATCATTTGTTTCCCACAATCTCAGTCCGTACAAGGCTACCATTATCTCTAAGTCAACTTTGTCTTCCACATATGATAGCGTTCTCGACAGCGCTCCTACAACCAAGAAGCTCAAATCAATTCGAGTGAACCAAAACCTTAGGACAAGGTCGAATATAAAGATGAAGCGAAAACCCTCGTCTAAGCCTGATCGACGATGTCGAACCCGATTTCAGGGCGATTCCAAAAGGGACAGGTGCGACCCTCCTTTGATTATGTCCGCAGAACCCGATGATTTGGATGTGCCAGTTGAGACATCTCCTGCTTCCGTTCCCCTACTAATGGTTGCCGCTATGACTGGGAAGCAAGATTTGATTGTTCCTTTGATTAAGGATGAGTCCATCCTTGTTGAGAGTAAGAAACTACTTGTGACTGCTGTTGTGGACTTGGCTAAGGTCATTCATTCCAAGGGTAATGGTTCATATCATCACTCTAATTTCAGTTTTCAACCTCACTCTCACACATTTGATGAGACACAGACGTCAAAGGGTAGCGAATTGGCTTTGGATGGGCTTTGGGATGCCACTTCTGGCTTGCCCACCATGATGATTAAATTTAAGAGTTTACACAACCTTGAGTTTGTGCCGGGAGGCTGTGAGATGATTCGTGGAAAAGACAACATGTTTGCAGATCATGAGCAGAGTGGAAGTGTGCATCACTTTCCTCAATCCTTGTCAGATGAGGCACACATCTCTCAGGTAACGATCACCAATTATGGATTGCAAGCTACTTTGTTGAGTCAAAGTTTGATTGGTTCACCTGTGATGTTCGCATTGTCAATACTTGAATCCTTCAAGCATGCAAGCACAGACACGTTGATTGAATTTGAGCAGTCCATATTGTGTTCCTTTGCTATTAGCCAATTTATGATAGCTCTTGGAGCAGCAAACCTACCAGTTAGTCTACTGAACTGGAAGGTTGGACTGGAAATTTCGCTTTCAAGTCGCGACGGAGAATATGATCAGCATCTTGCAAACTTGACCGAGAGCCCGAGCAGAAATGCCAAGAATCTATTTATTCATATGCATGAAGGGCCAATTGAAACCACTTCTTCGGCCCTGCACCATTGGGTCCAATCTTCTTTGGATGAGACTGCAACTCGTATCCTTGACCAGAAACTCAATTATCAGCAAGGATTCTCTTATTTTGGCATAGTTCCTGTTACTCTCGAAAAGGTTTTCGGCTTCTGTTCTAAACTACACTGTTTCAGCCACCTTACTGTAATTTTCAATGTTGGTATTGAGACTGTTGTGCAACCCTTTAATCGTGGATGGGGATTTCAAATTCATCAACTTGGGCCATCCTTGACTATTACTAGTCCTTTTGAGATGCTACATTTTGCTGACGGGCATCTTGCTTGCCAGAGCTATCGTTTAATTGGTCCCATTGGTTCCACGACGACAGTCATTACTTCCATAGTTGAGACCTGCTATCAACTACTACAACCCCAAATGTGCATTTCCATGTTTGCAGAAAATTTTACCAACTTGAGCCCATTTGTGCTTGTTATTTTTGACATTGAGATGCACATTCTAGACATTCTGTCTAGACCATTGTTTGGTACTAATTCTCTGAAGAAACAAAGGGCTATGCAAGGATCCCTAGTTGCATGTGTACACCCCAGCAATATGAGCACCATATATCGAAGTGCCACAATAGGTTTGCTGATTCCACATGCAAATCTCTTCCTCATTCTTTGTGGAACTACTCCAAAAAATATTCACCTTGCGACTCTAACTAGGGATTTGTGGTCTGTTCACATTTGGAAGGAGATTAACCCAACAAATTCCTACTATGGGTGTGAGCATTTTGGTATGGTACAAATCTTATTTGTCAATATAGTCGTCGATGGTTCAGTTGTAGCCCGAGTATCATGCCCTCTACATACAGTTGCCTGCGCCCTTTATAAGCATCATTCCATGGTTGTGGAAGCTGCTATAATACTTAGGTATATTTCTCTTCCACCGTCTTCATTGGATATGCTTATGACAGATGTTAGTGGAGGTAACCTTTCTCTAGTTGAAGTGGCTCTACTTCATTTCCCTTGCCAAGCTAAACCTCTCTGCCACCCAAGGAGGTTTATTGTTGATGAAGTTATTGACTTTCTCAAAAGCAATTCACAGTTATCTTTTCTGAGGTACTCTTCTGATGCACTTCTTTCAAGAGATAATTGTGTAGGCTTTGTGACTAATATGCACAAGTTTTTTCAATACATGGTGGGACATTTTCATAAGGATGAGTTTTTCATGGCAACGTGCTCTTCCACTATTTATCCTCCATTCCATGCACTTGTGAAGAAATTGTTTCTTTTATCACCTATGGTTGCTATCCATTCATTCCAACCACTTTTGGCTGACATATTGAGAGAAGATGCACAATTTATCTTTGGACTTATGATGGTTGTCTCTATGAATGAGCTGATGTTTTTGAGGTCTAAGAAACAAGGCCTTGAAATTGGGTATAGTGATATTGATAATGGACATTATTGTTCTTCTGTGAGCTGTTCTCTTCACTTGCCTAATTTCATGATTACATGGTTTTTTGAATTCACCTCTGTGGCAATTGACTTTGGAGATCTTGCATTAATTCCTCCAAAACACTTTTCAGTATCTATGCTGTCAAATGAGGCTCCCTCGCTTATCTTGTTGTTTAGAGTTATATACTCCCAGGCAATTGAATTTGTGAATTCAAACCATCTGTTGCAGGGTCCCAATAGAGCAGCCTTGAGAATCTTTTCTAATTCTCATGTGGGTGTTCATTTCTGTGCACTAATGCATTATTGTGTCTTCAAATATTATATATGTCTCTTGAAGCAAAGAATGGAGTTCCCCTGGAATTACATGCCACATAATGAGATAGCTGTCTTTCTTATTTGGATCATTGAGGCATCGTCAGGTTTCAAAGAACAGGCAAATATTGATACAACTCTTCACACTAATGTGGGAGCTTTTGACGCTACTAGAACTAGACTTGAGTTTGGTGCGCATATGAGAAATACCAATTCAGATGCTCGCATGTTTTCTTTTAGTGAGTGTCCATCAGAAGCAATGTGGTTGCCTGTAGACGTCATTGAAGCTCAATACATGGGAAAGCTCGGAATTTGGTTGGTTATTGCTTCAGGATCATATACTAAAGTTCAGGCTTCGAACATGCATTTTCTGGCCCTCGAATACAGTGGTAGTTCACTATATATATTGCAGAATGGTGTTGACCTCTCAGTAGTGAAGCTGATTAGTCTTATGCACATCTCCCATAAGAGAACAATTACTGCAACACATGCTCTTTCTACTCACAAGAAGCTGATGCTCACAGGCTATTACTATAACAATAGTACTCTTGGACACCGTACAGATACAAGAGCTACTAGAACTTGCAGTTTACAGTGTTCTTCACCAAGGCATGAGGATTGCCTTCTTTTAAGGGGGAGGATTGTTAGGATCACGATGTGTACTAGCCCAATAGTTATGCATGAGCCCATAAGTACTGAACCCATTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003724 GO:0003824 GO:0004004 GO:0004386 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005773 GO:0006139 GO:0006725 GO:0006807 GO:0008026 GO:0008150 GO:0008152 GO:0008186 GO:0009987 GO:0010501 GO:0016070 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0031974 GO:0031981 GO:0034641 GO:0042623 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0046483 GO:0070013 GO:0070035 GO:0071704 GO:0090304 GO:0140098 GO:1901360

KEGG Pathways

Metabolic & Signaling

ko03040 ko05202 ko05205 map03040 map05202 map05205

Pfam Domains

Protein Families

DEAD Helicase_C WW

Protein Analysis

1359

Amino Acids

152.6

Weight (kDa)

6.7

Isoelectric Point (pI)

49.27

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
WW	PF00397	79 - 109	3.8e-07	WW domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0017709)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_7g12460
rosa_chinensis	RchiOBHm_Chr4g0401881 RchiOBHm_Chr4g0402161 RchiOBHm_Chr6g0244351 RchiOBHm_Chr6g0269331
rosa_multiflora	Rmu_sc0004459.1_g000008 Rmu_sc0007380.1_g000010 Rmu_sc0008973.1_g000007
rosa_roxburghii	Rroxscaffold_1G00066910 Rroxscaffold_5G00359100 Rroxscaffold_6G00404090
rosa_rugosa	Rorug04G0254100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2490
AasI	GACNNNNNNGTC	1 cut(s)	1063
AatII	GACGTC	2 cut(s)	1067, 3651
Acc16I	TGCGCA	1 cut(s)	3567
Acc36I	ACCTGC	1 cut(s)	2018
AccB7I	CCANNNNNTGG	1 cut(s)	2141
AccBSI	CCGCTC	1 cut(s)	168
AccI	GTMKAC	2 cut(s)	1486, 3645
AccII	CGCG	1 cut(s)	1530
AciI	CCGC	3 cut(s)	166, 804, 873
AclWI	GGATC	8 cut(s)	5, 34, 221, 2175, 2188, 3467, 3712, 4028
AcuI	CTGAAG	2 cut(s)	2177, 3684
AcyI	GRCGYC	2 cut(s)	1064, 3648
AdeI	CACNNNGTG	1 cut(s)	4029
AfaI	GTAC	9 cut(s)	575, 2146, 2199, 2403, 2723, 3916, 3931, 4033, 4066
AfeI	AGCGCT	1 cut(s)	637
AfiI	CCNNNNNNNGG	5 cut(s)	251, 757, 865, 2141, 2379
AflIII	ACRYGT	2 cut(s)	1203, 1395
AhdI	GACNNNNNGTC	1 cut(s)	2125
AhlI	ACTAGT	1 cut(s)	1898
AjnI	CCWGG	2 cut(s)	3249, 3413
AjuI	GAANNNNNNNTTGG	2 cut(s)	3169, 3201
AleI	CACNNNNGTG	2 cut(s)	3137, 3521
Alw21I	GWGCWC	3 cut(s)	2219, 2843, 3352
Alw26I	GTCTC	7 cut(s)	830, 1049, 1667, 1820, 2000, 2992, 3392
Alw44I	GTGCAC	1 cut(s)	3348
AlwI	GGATC	8 cut(s)	5, 34, 221, 2175, 2188, 3467, 3712, 4028
Ama87I	CYCGRG	2 cut(s)	1573, 2448
Aor51HI	AGCGCT	1 cut(s)	637
AoxI	GGCC	6 cut(s)	323, 1616, 1638, 1880, 3028, 3744
ApaLI	GTGCAC	1 cut(s)	3348
ApeKI	GCWGC	3 cut(s)	1466, 2516, 3302
ArsI	GACNNNNNNTTYG	2 cut(s)	1190, 1222
AseI	ATTAAT	1 cut(s)	3167
Asp700I	GAANNNNTTC	1 cut(s)	3316
AspLEI	GCGC	3 cut(s)	638, 2483, 3568
AspS9I	GGNCC	8 cut(s)	206, 1616, 1639, 1654, 1880, 1964, 3290, 3745
AsuC2I	CCSGG	1 cut(s)	1170
AsuHPI	GGTGA	8 cut(s)	352, 473, 1282, 1332, 2297, 2889, 3124, 3966
AsuII	TTCGAA	1 cut(s)	3728
AvaI	CYCGRG	2 cut(s)	1573, 2448
AvaII	GGWCC	4 cut(s)	206, 1654, 1964, 3290
AxyI	CCTNAGG	1 cut(s)	683
BaeGI	GKGCMC	1 cut(s)	3352
BaeI	ACNNNNGTAYC	2 cut(s)	406, 439
BamHI	GGATCC	1 cut(s)	2180
BanII	GRGCYC	4 cut(s)	35, 1574, 2078, 4059
BarI	GAAGNNNNNNTAC	2 cut(s)	2598, 2630
BbsI	GAAGAC	3 cut(s)	600, 2544, 3361
Bbv12I	GWGCWC	3 cut(s)	2219, 2843, 3352
BbvI	GCAGC	3 cut(s)	1478, 2503, 3314
BcgI	CGANNNNNNTGC	2 cut(s)	2439, 2473
BciT130I	CCWGG	2 cut(s)	3251, 3415
BciVI	GTATCC	2 cut(s)	357, 1697
BclI	TGATCA	1 cut(s)	1543
BcnI	CCSGG	1 cut(s)	1170
BcoDI	GTCTC	7 cut(s)	830, 1049, 1667, 1820, 2000, 2992, 3392
BcuI	ACTAGT	1 cut(s)	1898
BfmI	CTRYAG	1 cut(s)	3642
BfoI	RGCGCY	1 cut(s)	639
BfuAI	ACCTGC	1 cut(s)	2018
BfuI	GTATCC	2 cut(s)	357, 1697
BglII	AGATCT	1 cut(s)	3157
BisI	GCNGC	4 cut(s)	873, 1467, 2517, 3303
BlsI	GCNGC	4 cut(s)	874, 1468, 2518, 3304
BmcAI	AGTACT	2 cut(s)	3916, 4066
Bme1390I	CCNGG	3 cut(s)	1170, 3251, 3415
Bme18I	GGWCC	4 cut(s)	206, 1654, 1964, 3290
BmeRI	GACNNNNNGTC	1 cut(s)	2125
BmeT110I	CYCGRG	2 cut(s)	1573, 2448
BmgT120I	GGNCC	8 cut(s)	206, 1616, 1639, 1654, 1880, 1964, 3290, 3745
BmiI	GGNNCC	7 cut(s)	1655, 1966, 1975, 2182, 3213, 3291, 3292
BmrFI	CCNGG	3 cut(s)	1170, 3251, 3415
BmrI	ACTGGG	2 cut(s)	307, 891
BmuI	ACTGGG	2 cut(s)	307, 891
BoxI	GACNNNNGTC	1 cut(s)	1985
BpiI	GAAGAC	3 cut(s)	600, 2544, 3361
BplI	GAGNNNNNCTC	4 cut(s)	3073, 3105, 3200, 3232
BpmI	CTGGAG	1 cut(s)	204
Bpu10I	CCTNAGC	1 cut(s)	975
Bpu14I	TTCGAA	1 cut(s)	3728
BpuEI	CTTGAG	4 cut(s)	1178, 2092, 3328, 3575
BpuMI	CCSGG	1 cut(s)	1170
Bsa29I	ATCGAT	1 cut(s)	261
BsaBI	GATNNNNATC	2 cut(s)	9, 1548
BsaHI	GRCGYC	2 cut(s)	1064, 3648
BsaI	GGTCTC	1 cut(s)	2000
BsaJI	CCNNGG	8 cut(s)	209, 245, 990, 2502, 2654, 3249, 3413, 3977
BsaWI	WCCGGW	1 cut(s)	64
Bsc4I	CCNNNNNNNGG	5 cut(s)	251, 757, 865, 2141, 2379
Bse1I	ACTGG	7 cut(s)	293, 313, 830, 886, 1478, 1499, 1512
Bse21I	CCTNAGG	1 cut(s)	683
Bse3DI	GCAATG	1 cut(s)	3636
Bse8I	GATNNNNATC	2 cut(s)	9, 1548
BseBI	CCWGG	2 cut(s)	3251, 3415
BseCI	ATCGAT	1 cut(s)	261
BseDI	CCNNGG	8 cut(s)	209, 245, 990, 2502, 2654, 3249, 3413, 3977
BseGI	GGATG	9 cut(s)	829, 922, 924, 1096, 1108, 1675, 1859, 1883, 2824
BseJI	GATNNNNATC	2 cut(s)	9, 1548
BseLI	CCNNNNNNNGG	5 cut(s)	251, 757, 865, 2141, 2379
BseMI	GCAATG	1 cut(s)	3636
BseMII	CTCAG	5 cut(s)	381, 580, 1292, 2708, 3813
BseNI	ACTGG	7 cut(s)	293, 313, 830, 886, 1478, 1499, 1512
BseRI	GAGGAG	2 cut(s)	355, 420
BseSI	GKGCMC	1 cut(s)	3352
BseXI	GCAGC	3 cut(s)	1478, 2503, 3314
BseYI	CCCAGC	1 cut(s)	2204
BsgI	GTGCAG	1 cut(s)	1628
Bsh1236I	CGCG	1 cut(s)	1530
BshFI	GGCC	6 cut(s)	325, 1618, 1640, 1882, 3030, 3746
BshVI	ATCGAT	1 cut(s)	261
BsiHKAI	GWGCWC	3 cut(s)	2219, 2843, 3352
BsiHKCI	CYCGRG	2 cut(s)	1573, 2448
BsiSI	CCGG	2 cut(s)	65, 1169
BslFI	GGGAC	4 cut(s)	788, 1950, 2817, 3276
BslI	CCNNNNNNNGG	5 cut(s)	251, 757, 865, 2141, 2379
BsmAI	GTCTC	7 cut(s)	830, 1049, 1667, 1820, 2000, 2992, 3392
BsmFI	GGGAC	4 cut(s)	788, 1950, 2817, 3276
BsnI	GGCC	6 cut(s)	325, 1618, 1640, 1882, 3030, 3746
Bso31I	GGTCTC	1 cut(s)	2000
BsoBI	CYCGRG	2 cut(s)	1573, 2448
Bsp119I	TTCGAA	1 cut(s)	3728
Bsp1286I	GDGCHC	7 cut(s)	35, 1574, 2078, 2219, 2843, 3352, 4059
Bsp1407I	TGTACA	1 cut(s)	2197
Bsp19I	CCATGG	2 cut(s)	209, 2502
Bsp68I	TCGCGA	1 cut(s)	1530
BspACI	CCGC	3 cut(s)	166, 804, 873
BspANI	GGCC	6 cut(s)	325, 1618, 1640, 1882, 3030, 3746
BspCNI	CTCAG	5 cut(s)	380, 579, 1291, 2709, 3812
BspDI	ATCGAT	1 cut(s)	261
BspFNI	CGCG	1 cut(s)	1530
BspHI	TCATGA	2 cut(s)	1216, 3108
BspLI	GGNNCC	7 cut(s)	1655, 1966, 1975, 2182, 3213, 3291, 3292
BspMI	ACCTGC	1 cut(s)	2018
BspPI	GGATC	8 cut(s)	5, 34, 221, 2175, 2188, 3467, 3712, 4028
BspQI	GCTCTTC	1 cut(s)	2848
BspT104I	TTCGAA	1 cut(s)	3728
BspTNI	GGTCTC	1 cut(s)	2000
BsrBI	CCGCTC	1 cut(s)	168
BsrDI	GCAATG	1 cut(s)	3636
BsrGI	TGTACA	1 cut(s)	2197
BsrI	ACTGG	7 cut(s)	293, 313, 830, 886, 1478, 1499, 1512
BssECI	CCNNGG	8 cut(s)	209, 245, 990, 2502, 2654, 3249, 3413, 3977
BssNI	GRCGYC	2 cut(s)	1064, 3648
BssT1I	CCWWGG	6 cut(s)	209, 245, 990, 2502, 2654, 3977
Bst2UI	CCWGG	2 cut(s)	3251, 3415
Bst6I	CTCTTC	6 cut(s)	2273, 2547, 2731, 2848, 3096, 3516
BstACI	GRCGYC	2 cut(s)	1064, 3648
BstAPI	GCANNNNNTGC	1 cut(s)	3860
BstAUI	TGTACA	1 cut(s)	2197
BstBI	TTCGAA	1 cut(s)	3728
BstC8I	GCNNGC	7 cut(s)	1118, 1308, 1383, 1387, 1942, 2479, 3595
BstDSI	CCRYGG	2 cut(s)	209, 2502
BstEII	GGTNACC	1 cut(s)	2588
BstF5I	GGATG	9 cut(s)	829, 922, 924, 1096, 1108, 1675, 1859, 1883, 2824
BstFNI	CGCG	1 cut(s)	1530
BstH2I	RGCGCY	1 cut(s)	639
BstHHI	GCGC	3 cut(s)	638, 2483, 3568
BstMAI	GTCTC	7 cut(s)	830, 1049, 1667, 1820, 2000, 2992, 3392
BstMWI	GCNNNNNNNGC	5 cut(s)	1463, 1615, 2214, 3536, 3860
BstNI	CCWGG	2 cut(s)	3251, 3415
BstNSI	RCATGY	8 cut(s)	1207, 1385, 2196, 2261, 3427, 3601, 3736, 3863
BstPAI	GACNNNNGTC	1 cut(s)	1985
BstPI	GGTNACC	1 cut(s)	2588
BstSCI	CCNGG	3 cut(s)	1168, 3249, 3413
BstSFI	CTRYAG	1 cut(s)	3642
BstSLI	GKGCMC	1 cut(s)	3352
BstUI	CGCG	1 cut(s)	1530
BstV1I	GCAGC	3 cut(s)	1478, 2503, 3314
BstV2I	GAAGAC	3 cut(s)	600, 2544, 3361
BstX2I	RGATCY	3 cut(s)	226, 2180, 3157
BstXI	CCANNNNNNTGG	2 cut(s)	246, 2509
BstYI	RGATCY	3 cut(s)	226, 2180, 3157
Bsu15I	ATCGAT	1 cut(s)	261
Bsu36I	CCTNAGG	1 cut(s)	683
BsuI	GTATCC	2 cut(s)	357, 1697
BsuRI	GGCC	6 cut(s)	325, 1618, 1640, 1882, 3030, 3746
BsuTUI	ATCGAT	1 cut(s)	261
BtgI	CCRYGG	2 cut(s)	209, 2502
BtsCI	GGATG	9 cut(s)	829, 922, 924, 1096, 1108, 1675, 1859, 1883, 2824
BtsIMutI	CAGTG	4 cut(s)	208, 1783, 3763, 3971
BtuMI	TCGCGA	1 cut(s)	1530
BveI	ACCTGC	1 cut(s)	2018
Cac8I	GCNNGC	7 cut(s)	1118, 1308, 1383, 1387, 1942, 2479, 3595
CciI	TCATGA	2 cut(s)	1216, 3108
CfoI	GCGC	3 cut(s)	638, 2483, 3568
Cfr13I	GGNCC	8 cut(s)	206, 1616, 1639, 1654, 1880, 1964, 3290, 3745
ClaI	ATCGAT	1 cut(s)	261
CseI	GACGC	1 cut(s)	3545
Csp6I	GTAC	9 cut(s)	574, 2145, 2198, 2402, 2722, 3915, 3930, 4032, 4065
CviQI	GTAC	9 cut(s)	574, 2145, 2198, 2402, 2722, 3915, 3930, 4032, 4065
DraIII	CACNNNGTG	1 cut(s)	4029
DrdI	GACNNNNNNGTC	1 cut(s)	1063
DriI	GACNNNNNGTC	1 cut(s)	2125
DseDI	GACNNNNNNGTC	1 cut(s)	1063
Eam1104I	CTCTTC	6 cut(s)	2273, 2547, 2731, 2848, 3096, 3516
Eam1105I	GACNNNNNGTC	1 cut(s)	2125
EarI	CTCTTC	6 cut(s)	2273, 2547, 2731, 2848, 3096, 3516
Eco130I	CCWWGG	6 cut(s)	209, 245, 990, 2502, 2654, 3977
Eco147I	AGGCCT	1 cut(s)	3030
Eco24I	GRGCYC	4 cut(s)	35, 1574, 2078, 4059
Eco31I	GGTCTC	1 cut(s)	2000
Eco47I	GGWCC	4 cut(s)	206, 1654, 1964, 3290
Eco47III	AGCGCT	1 cut(s)	637
Eco57I	CTGAAG	2 cut(s)	2177, 3684
Eco81I	CCTNAGG	1 cut(s)	683
Eco88I	CYCGRG	2 cut(s)	1573, 2448
Eco91I	GGTNACC	1 cut(s)	2588
EcoO109I	RGGNCCY	1 cut(s)	3290
EcoO65I	GGTNACC	1 cut(s)	2588
EcoRI	GAATTC	2 cut(s)	3128, 3268
EcoRII	CCWGG	2 cut(s)	3249, 3413
EcoT14I	CCWWGG	6 cut(s)	209, 245, 990, 2502, 2654, 3977
EcoT22I	ATGCAT	4 cut(s)	1611, 3360, 3738, 4053
EcoT38I	GRGCYC	4 cut(s)	35, 1574, 2078, 4059
ErhI	CCWWGG	6 cut(s)	209, 245, 990, 2502, 2654, 3977
FaqI	GGGAC	4 cut(s)	788, 1950, 2817, 3276
FauNDI	CATATG	4 cut(s)	45, 616, 1605, 3570
FbaI	TGATCA	1 cut(s)	1543
FblI	GTMKAC	2 cut(s)	1486, 3645
Fnu4HI	GCNGC	4 cut(s)	873, 1467, 2517, 3303
FokI	GGATG	9 cut(s)	836, 911, 929, 1103, 1115, 1682, 1866, 1870, 2831
FriOI	GRGCYC	4 cut(s)	35, 1574, 2078, 4059
Fsp4HI	GCNGC	4 cut(s)	873, 1467, 2517, 3303
FspAI	RTGCGCAY	1 cut(s)	3567
FspI	TGCGCA	1 cut(s)	3567
GlaI	GCGC	3 cut(s)	637, 2482, 3567
GluI	GCNGC	4 cut(s)	873, 1467, 2517, 3303
GsaI	CCCAGC	1 cut(s)	2208
GsuI	CTGGAG	1 cut(s)	204
HaeII	RGCGCY	1 cut(s)	639
HaeIII	GGCC	6 cut(s)	325, 1618, 1640, 1882, 3030, 3746
HapII	CCGG	2 cut(s)	65, 1169
HgaI	GACGC	1 cut(s)	3545
HhaI	GCGC	3 cut(s)	638, 2483, 3568
Hin1I	GRCGYC	2 cut(s)	1064, 3648
Hin6I	GCGC	3 cut(s)	636, 2481, 3566
HinP1I	GCGC	3 cut(s)	636, 2481, 3566
HincII	GTYRAC	2 cut(s)	600, 3793
HindII	GTYRAC	2 cut(s)	600, 3793
HpaII	CCGG	2 cut(s)	65, 1169
HphI	GGTGA	8 cut(s)	352, 473, 1282, 1332, 2297, 2889, 3124, 3966
Hpy99I	CGWCG	5 cut(s)	153, 741, 1535, 1984, 2432
HpyAV	CCTTC	7 cut(s)	45, 244, 1384, 1492, 1607, 2344, 4004
HpyCH4IV	ACGT	4 cut(s)	1064, 1397, 2837, 3648
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1463, 1615, 2214, 3536, 3860
HpySE526I	ACGT	4 cut(s)	1064, 1397, 2837, 3648
Hsp92I	GRCGYC	2 cut(s)	1064, 3648
HspAI	GCGC	3 cut(s)	636, 2481, 3566
KflI	GGGWCCC	1 cut(s)	3290
Ksp22I	TGATCA	1 cut(s)	1543
LguI	GCTCTTC	1 cut(s)	2848
LmnI	GCTCC	5 cut(s)	223, 643, 1463, 3217, 3527
Lsp1109I	GCAGC	3 cut(s)	1478, 2503, 3314
MaeII	ACGT	4 cut(s)	1064, 1397, 2837, 3648
MaeIII	GTNAC	5 cut(s)	952, 1282, 1744, 2588, 2767
MbiI	CCGCTC	1 cut(s)	168
MfeI	CAATTG	3 cut(s)	1620, 3144, 3255
MflI	RGATCY	3 cut(s)	226, 2180, 3157
MhlI	GDGCHC	7 cut(s)	35, 1574, 2078, 2219, 2843, 3352, 4059
MlyI	GAGTC	3 cut(s)	929, 1330, 2308
MmeI	TCCRAC	4 cut(s)	131, 203, 1483, 2947
Mph1103I	ATGCAT	4 cut(s)	1611, 3360, 3738, 4053
MroXI	GAANNNNTTC	1 cut(s)	3316
MseI	TTAA	9 cut(s)	257, 912, 1134, 1140, 1845, 1958, 2358, 3167, 4001
MspI	CCGG	2 cut(s)	65, 1169
MspR9I	CCNGG	3 cut(s)	1170, 3251, 3415
MunI	CAATTG	3 cut(s)	1620, 3144, 3255
MvaI	CCWGG	2 cut(s)	3251, 3415
MvnI	CGCG	1 cut(s)	1530
MwoI	GCNNNNNNNGC	5 cut(s)	1463, 1615, 2214, 3536, 3860
NciI	CCSGG	1 cut(s)	1170
NcoI	CCATGG	2 cut(s)	209, 2502
NdeI	CATATG	4 cut(s)	45, 616, 1605, 3570
NlaIV	GGNNCC	7 cut(s)	1655, 1966, 1975, 2182, 3213, 3291, 3292
NmuCI	GTSAC	2 cut(s)	952, 2767
NruI	TCGCGA	1 cut(s)	1530
NsbI	TGCGCA	1 cut(s)	3567
NsiI	ATGCAT	4 cut(s)	1611, 3360, 3738, 4053
NspI	RCATGY	8 cut(s)	1207, 1385, 2196, 2261, 3427, 3601, 3736, 3863
NspV	TTCGAA	1 cut(s)	3728
OliI	CACNNNNGTG	2 cut(s)	3137, 3521
PaeI	GCATGC	1 cut(s)	1385
PagI	TCATGA	2 cut(s)	1216, 3108
PceI	AGGCCT	1 cut(s)	3030
PciI	ACATGT	1 cut(s)	1203
PciSI	GCTCTTC	1 cut(s)	2848
PcsI	WCGNNNNNNNCGW	1 cut(s)	742
PdmI	GAANNNNTTC	1 cut(s)	3316
PflFI	GACNNNGTC	2 cut(s)	691, 741
PflMI	CCANNNNNTGG	1 cut(s)	2141
PkrI	GCNGC	4 cut(s)	874, 1468, 2518, 3304
PleI	GAGTC	3 cut(s)	928, 1329, 2308
PpsI	GAGTC	3 cut(s)	928, 1329, 2308
PpuMI	RGGWCCY	1 cut(s)	3290
PscI	ACATGT	1 cut(s)	1203
PshAI	GACNNNNGTC	1 cut(s)	1985
PshBI	ATTAAT	1 cut(s)	3167
PsiI	TTATAA	1 cut(s)	2490
Psp5II	RGGWCCY	1 cut(s)	3290
Psp6I	CCWGG	2 cut(s)	3249, 3413
PspEI	GGTNACC	1 cut(s)	2588
PspFI	CCCAGC	1 cut(s)	2204
PspGI	CCWGG	2 cut(s)	3249, 3413
PspN4I	GGNNCC	7 cut(s)	1655, 1966, 1975, 2182, 3213, 3291, 3292
PspPI	GGNCC	8 cut(s)	206, 1616, 1639, 1654, 1880, 1964, 3290, 3745
PspPPI	RGGWCCY	1 cut(s)	3290
PsuI	RGATCY	3 cut(s)	226, 2180, 3157
PsyI	GACNNNGTC	2 cut(s)	691, 741
RruI	TCGCGA	1 cut(s)	1530
RsaI	GTAC	9 cut(s)	575, 2146, 2199, 2403, 2723, 3916, 3931, 4033, 4066
RsaNI	GTAC	9 cut(s)	574, 2145, 2198, 2402, 2722, 3915, 3930, 4032, 4065
SapI	GCTCTTC	1 cut(s)	2848
SaqAI	TTAA	9 cut(s)	257, 912, 1134, 1140, 1845, 1958, 2358, 3167, 4001
SatI	GCNGC	4 cut(s)	873, 1467, 2517, 3303
Sau96I	GGNCC	8 cut(s)	206, 1616, 1639, 1654, 1880, 1964, 3290, 3745
ScaI	AGTACT	2 cut(s)	3916, 4066
SchI	GAGTC	3 cut(s)	929, 1330, 2308
ScrFI	CCNGG	3 cut(s)	1170, 3251, 3415
SduI	GDGCHC	7 cut(s)	35, 1574, 2078, 2219, 2843, 3352, 4059
SfcI	CTRYAG	1 cut(s)	3642
SfuI	TTCGAA	1 cut(s)	3728
SinI	GGWCC	4 cut(s)	206, 1654, 1964, 3290
SmlI	CTYRAG	4 cut(s)	1157, 2071, 3307, 3554
SmoI	CTYRAG	4 cut(s)	1157, 2071, 3307, 3554
SpeI	ACTAGT	1 cut(s)	1898
SphI	GCATGC	1 cut(s)	1385
SseBI	AGGCCT	1 cut(s)	3030
SsiI	CCGC	3 cut(s)	166, 804, 873
SspI	AATATT	3 cut(s)	2302, 3377, 3499
StuI	AGGCCT	1 cut(s)	3030
StyD4I	CCNGG	3 cut(s)	1168, 3249, 3413
StyI	CCWWGG	6 cut(s)	209, 245, 990, 2502, 2654, 3977
TaiI	ACGT	4 cut(s)	1067, 1400, 2840, 3651
TaqII	GACCGA	1 cut(s)	1580
TatI	WGTACW	4 cut(s)	2197, 3914, 4031, 4064
TauI	GCSGC	1 cut(s)	875
Tru1I	TTAA	9 cut(s)	257, 912, 1134, 1140, 1845, 1958, 2358, 3167, 4001
Tru9I	TTAA	9 cut(s)	257, 912, 1134, 1140, 1845, 1958, 2358, 3167, 4001
TscAI	CASTG	4 cut(s)	208, 1790, 3763, 3971
TseFI	GTSAC	2 cut(s)	952, 2767
TseI	GCWGC	3 cut(s)	1466, 2516, 3302
Tsp45I	GTSAC	2 cut(s)	952, 2767
TspGWI	ACGGA	4 cut(s)	397, 561, 841, 1548
TspRI	CASTG	4 cut(s)	208, 1790, 3763, 3971
Tth111I	GACNNNGTC	2 cut(s)	691, 741
Van91I	CCANNNNNTGG	1 cut(s)	2141
VneI	GTGCAC	1 cut(s)	3348
VpaK11BI	GGWCC	4 cut(s)	206, 1654, 1964, 3290
VspI	ATTAAT	1 cut(s)	3167
XbaI	TCTAGA	2 cut(s)	2116, 2129
XceI	RCATGY	8 cut(s)	1207, 1385, 2196, 2261, 3427, 3601, 3736, 3863
XmiI	GTMKAC	2 cut(s)	1486, 3645
XmnI	GAANNNNTTC	1 cut(s)	3316
ZraI	GACGTC	2 cut(s)	1065, 3649
ZrmI	AGTACT	2 cut(s)	3916, 4066
Zsp2I	ATGCAT	4 cut(s)	1611, 3360, 3738, 4053

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.