Rmu_sc0004481.1_g000026
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004481.1
OG0016498
Physical Location & Seq
Reverse (-)
99017 .. 104321
5305 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3528 bp
atgtcaactaatgcaatcactattattctcttgtttctcaacatctctctatttcctgccgtctctgctctgaaccaagagggtctctccctgctttcatggctttcaaccttcaactcttcttcctcagctgcccttttttcttcctggaatccaactgcctatgatccatgcaaatgggattatatcagatgttctgaagcaggatttgtttcagaaatcacaataacctcaatcaatatacaaaccaggttccccatccagctactcacctttaatcacctcaccacccttgtaatctcaaacgccaacctcagcggagaaattccacaggcaattggaaacttgtcatcactcatcaccctgaatgtcagctccaatgctctacgaggttacatcccacaggagataggaaaattatcacaactgcagtggttatcactgaattcaaattccttggatggtgcaattccaaaagaaattggaaactgttcagagcttcaacgacttgaactgtacgataaccagctatctggaaagattcctgctgaaataggccagttgtgggctctggaaatcttccgtgctggcgggaatcctgacattcaaggtgaaattccaatgcagatatcaaactgcaagggactaactttccttggccttgcagctacaggaatcacggggaaaattccaagcagcgtaggagaacttaagaatctccaaactctttcaatttatactgcaaatctctcaggtgagattccaccagaaattggtaactgttcagccttagaaaatttgtttctgtatgataaccagcttgctggtcaaattcctggtgaacttggtctgttgaaaaaactcagaagagtgctactgtggaagaacaatctcaatggaagcatcccagacacactagggagctgttcaaaattgatagtagtcgatttctcgttgaatcttctgagtggtgaggttcctttgtctcttgcaaatttggtggcattggaggagcttcttctatctgagaatcagatatcaggcagtataccgcctttacttggaaacttttccagtttaaagcaactggaattggataacaacagattttcgggtacaattccacctgtcattgggcaactaaaagaacttactcttttctttgcctggcagaatcagctccaaggaagcataccaactgagctggccaactgtcagaaacttcaagcattggatctctcacataatctcctcagtggatcagtgccgagttctctgtttgacctcaagaacttaacacaactgttgctgatttccaatgaattttcaggacaacttccaaccaatattggtaactgtaccaatctgatccgcttacgcctcggatcaaacaacttcacaggtcagataccatccggaattcggctcctgcagagattaagcttcctagaattgtcagaaaatcattttactgggaaaattccttctgaaataggagactgctcccacctagaaatggttgatttgcatggaaatgagctccgaggcaggattccttcgtctttccaattccttactggtcttaatgttttagacctgtccatgaaccaaatagaaggcacagttcctgaacatttggggaagctgaaatccttgaacaaactggtgctcaatggaaactacattactggttccattcccaagtcactgagcctctgtagggatttgcagttgttggaactgagcagcaacagacttacaggctctatcccagacgagattggacacttgcaagggctcgatattctcttgaatctgagctggaattctctgacgggccccattccagaaagcttctcaaatctctcaaaacttgccactatggatctctcccacaatatgctgacaggaagcctgaaagtgctgggggttctggacaacctcgtctccctgaatgtctcatataatgacttttcaggtctacttcccagtactaattttttcaaggatcttccaccaactgcatttgctggtaatcacgagttatgcatcaacggaaacaagtgccaaccaaatagaaacctgcatgacaagaaatccatcagatatctaattatttgtatccttctcagtgtaactgcaactatatttcttatgcttgctggaatagctttgtttgtcagagtctggggaatcacatttgggatgacgaacggtaaagaagaaaatgatctggtgtgggatttcaccccatttcaaaagcttgattttgcagttaatgacattgtgactaagctatcagactcgaacattgttgggaagggaggctcaggaatggtttatcgtgtagagactccaacgagacaggtcattgcagtgaagaagttatggccgataaataatggggagcttccagaaagagaccttttctctacagaagttcatactcttggatcgataaggcataagaatatagtgaggcttctagcctgttgtaaaaatgggaaaaccagattgcttttgtttgattacatcagtaatgggagcttagctggattgctccatgagaagaggctgttcttggactgggatacaaggtacaagattgtactgggagcagctcatggcctagcttatcttcatcatgattgtgttcctccaatcattcatcgagatattaaggcgaacaacatcttggtagggccacagtatgaatctcttcttgcagattttggccttgcaaagctgttgagttctccagaatgctacaaagtttccaacacagttgcaggttcttatgggtacattgctccagaatatggatacagtttgaggatcacagaaaagagtgacgtgtatagctatggagtggtgctcctagaggtcctaacagggaaggaaccaacagataacaagattccagagggtgatcatattgtcacttgggttaataaggaacttagagagagaaaaagagaattcacatcaattgttgatcatcagctaatactgagatcagggacacaaattcaagaaatgcttcaagttcttggggtagctcttctctgtgttaatccctgcccggaggaaaggccgacaatgaaagatgtaacagcaatgctcaatgaaatcaagcatgaaaatgaagattgtgaaaaaccaaattttctcggccaaggagctgtgactaacaggaaaactgcaaatggccttttgaagcgtattgaggagaagaaacaggagcgtgattcttttgaaagagatgttttaaactgtaacctttctcgcattgatgagaaagaaaaaaacatgcgaattgaggttgacagaaaggcaaatcagcttgctgttagagaatttgattcaaccattcgtcaaaagcagagtgaggtgtttagcattgaacaaatgatcactgcagttactcgagagaagaacgtcttggatggtgatagggatgacagaataatactgtctcacaagaagacagactttgagactcagaaaaagaaacacagaaagatgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1175

Amino Acids

130.15

Weight (kDa)

6.12

Isoelectric Point (pI)

28.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 66 4.5e-07 Leucine rich repeat N-terminal domain
LRR_14 PF23598 92 - 194 8.7e-11 Leucine-rich repeat region
LRR_8 PF13855 94 - 153 6.6e-08 Leucine rich repeat
LRR_14 PF23598 205 - 304 1.9e-08 Leucine-rich repeat region
LRR_14 PF23598 475 - 560 9.7e-08 Leucine-rich repeat region
LRR_8 PF13855 502 - 562 1.3e-08 Leucine rich repeat
Pkinase PF00069 769 - 1038 6e-42 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 770 - 1040 3.2e-38 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016498)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g14410
malus_domestica MD05G1018000.v1.1
prunus_persica Prupe.8G022000_v2.0.a1
pyrus_communis pycom05g01070
rosa_chinensis RchiOBHm_Chr6g0276741
rosa_laevigata RLG00000013367
rosa_multiflora Rmu_sc0004481.1_g000026
rosa_roxburghii Rroxscaffold_7G00192010
rosa_rugosa Rorug06G0103400
rosa_samantha Rh6AG213800 Rh6BG218600 Rh6CG221600 Rh6DG211300
rosa_wichuraiana Rw6G018690

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1943
Acc36I ACCTGC 2 cut(s) 2091, 2780
AccB7I CCANNNNNTGG 2 cut(s) 773, 2819
AccI GTMKAC 2 cut(s) 1048, 1981
AccIII TCCGGA 1 cut(s) 1415
AciI CCGC 4 cut(s) 318, 591, 1052, 1372
AclWI GGATC 9 cut(s) 161, 1242, 1267, 1363, 1393, 1893, 2016, 2461, 2843
AcoI YGGCCR 3 cut(s) 1206, 2390, 3172
AcuI CTGAAG 1 cut(s) 219
AfaI GTAC 7 cut(s) 518, 1117, 1360, 1993, 2600, 2610, 2804
AfiI CCNNNNNNNGG 8 cut(s) 565, 773, 1061, 1133, 1422, 1516, 1720, 2819
AflII CTTAAG 1 cut(s) 710
AflIII ACRYGT 1 cut(s) 2853
AjiI CACGTC 1 cut(s) 2854
AjnI CCWGG 4 cut(s) 146, 248, 835, 1166
AjuI GAANNNNNNNTTGG 4 cut(s) 2678, 2710, 3425, 3457
Alw21I GWGCWC 3 cut(s) 1542, 1671, 2877
AlwI GGATC 9 cut(s) 161, 1242, 1267, 1363, 1393, 1893, 2016, 2461, 2843
AlwNI CAGNNNCTG 2 cut(s) 1628, 2187
Ama87I CYCGRG 1 cut(s) 3426
Aor13HI TCCGGA 1 cut(s) 1415
ApaI GGGCCC 1 cut(s) 1841
ApeKI GCWGC 5 cut(s) 131, 665, 696, 1746, 2618
Asp700I GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
AspS9I GGNCC 4 cut(s) 1837, 1838, 2702, 2884
AsuC2I CCSGG 1 cut(s) 3083
AvaI CYCGRG 1 cut(s) 3426
AvaII GGWCC 1 cut(s) 2884
BaeGI GKGCMC 1 cut(s) 1841
BalI TGGCCA 1 cut(s) 1208
BanII GRGCYC 4 cut(s) 571, 1542, 1800, 1841
BauI CACGAG 1 cut(s) 2039
BbsI GAAGAC 1 cut(s) 3491
Bbv12I GWGCWC 3 cut(s) 1542, 1671, 2877
BbvCI CCTCAGC 2 cut(s) 127, 314
BbvI GCAGC 5 cut(s) 118, 677, 708, 1758, 2630
BccI CCATC 5 cut(s) 266, 455, 1420, 2108, 3440
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 4 cut(s) 148, 250, 837, 1168
BciVI GTATCC 3 cut(s) 2132, 2584, 2816
BclI TGATCA 3 cut(s) 2929, 2995, 3411
BcnI CCSGG 1 cut(s) 3083
BfaI CTAG 6 cut(s) 917, 1448, 1511, 2486, 2630, 2879
BfmI CTRYAG 6 cut(s) 428, 669, 1430, 1717, 2433, 3417
BfrI CTTAAG 1 cut(s) 710
BfuAI ACCTGC 2 cut(s) 2091, 2780
BfuI GTATCC 3 cut(s) 2132, 2584, 2816
BisI GCNGC 5 cut(s) 132, 666, 697, 1747, 2619
BlpI GCTNAGC 1 cut(s) 2548
BlsI GCNGC 5 cut(s) 133, 667, 698, 1748, 2620
BmcAI AGTACT 1 cut(s) 1993
Bme1390I CCNGG 5 cut(s) 148, 250, 837, 1168, 3083
Bme18I GGWCC 1 cut(s) 2884
BmeT110I CYCGRG 1 cut(s) 3426
BmgBI CACGTC 1 cut(s) 2854
BmgT120I GGNCC 4 cut(s) 1837, 1838, 2702, 2884
BmiI GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1839, 1840, 2901
BmrFI CCNGG 5 cut(s) 148, 250, 837, 1168, 3083
BmrI ACTGGG 4 cut(s) 1482, 1983, 2596, 2621
BmsI GCATC 2 cut(s) 912, 2058
BmuI ACTGGG 4 cut(s) 1482, 1983, 2596, 2621
BpiI GAAGAC 1 cut(s) 3491
BplI GAGNNNNNCTC 4 cut(s) 71, 103, 2859, 2891
BpmI CTGGAG 2 cut(s) 2742, 2796
Bpu10I CCTNAGC 3 cut(s) 127, 314, 2329
Bpu1102I GCTNAGC 1 cut(s) 2548
BpuEI CTTGAG 1 cut(s) 1271
BpuMI CCSGG 1 cut(s) 3083
Bsa29I ATCGAT 1 cut(s) 2456
BsaI GGTCTC 2 cut(s) 89, 2415
BsaJI CCNNGG 6 cut(s) 456, 655, 1183, 1381, 1543, 3175
BsaWI WCCGGW 1 cut(s) 1415
BsaXI ACNNNNNCTCC 4 cut(s) 1233, 1263, 1931, 1961
Bsc4I CCNNNNNNNGG 8 cut(s) 565, 773, 1061, 1133, 1422, 1516, 1720, 2819
Bse3DI GCAATG 3 cut(s) 2370, 2805, 3123
BseAI TCCGGA 1 cut(s) 1415
BseBI CCWGG 4 cut(s) 148, 250, 837, 1168
BseCI ATCGAT 1 cut(s) 2456
BseDI CCNNGG 6 cut(s) 456, 655, 1183, 1381, 1543, 3175
BseGI GGATG 8 cut(s) 258, 396, 466, 903, 1412, 2211, 3451, 3463
BseLI CCNNNNNNNGG 8 cut(s) 565, 773, 1061, 1133, 1422, 1516, 1720, 2819
BseMI GCAATG 3 cut(s) 2370, 2805, 3123
BseRI GAGGAG 3 cut(s) 1025, 1241, 3242
BseSI GKGCMC 1 cut(s) 1841
BseXI GCAGC 5 cut(s) 118, 677, 708, 1758, 2630
BseYI CCCAGC 1 cut(s) 1924
BshVI ATCGAT 1 cut(s) 2456
BsiHKAI GWGCWC 3 cut(s) 1542, 1671, 2877
BsiHKCI CYCGRG 1 cut(s) 3426
BsiSI CCGG 2 cut(s) 1416, 3083
BslFI GGGAC 2 cut(s) 657, 3034
BslI CCNNNNNNNGG 8 cut(s) 565, 773, 1061, 1133, 1422, 1516, 1720, 2819
BsmBI CGTCTC 2 cut(s) 67, 1951
BsmFI GGGAC 2 cut(s) 657, 3034
BsmI GAATGC 1 cut(s) 2768
Bso31I GGTCTC 2 cut(s) 89, 2415
BsoBI CYCGRG 1 cut(s) 3426
Bsp120I GGGCCC 1 cut(s) 1837
Bsp1286I GDGCHC 6 cut(s) 571, 1542, 1671, 1800, 1841, 2877
Bsp13I TCCGGA 1 cut(s) 1415
Bsp1720I GCTNAGC 1 cut(s) 2548
BspACI CCGC 4 cut(s) 318, 591, 1052, 1372
BspDI ATCGAT 1 cut(s) 2456
BspEI TCCGGA 1 cut(s) 1415
BspHI TCATGA 1 cut(s) 2644
BspLI GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1839, 1840, 2901
BspMAI CTGCAG 3 cut(s) 432, 1434, 3421
BspMI ACCTGC 2 cut(s) 2091, 2780
BspPI GGATC 9 cut(s) 161, 1242, 1267, 1363, 1393, 1893, 2016, 2461, 2843
BspQI GCTCTTC 1 cut(s) 3066
BspTI CTTAAG 1 cut(s) 710
BspTNI GGTCTC 2 cut(s) 89, 2415
BsrDI GCAATG 3 cut(s) 2370, 2805, 3123
BssECI CCNNGG 6 cut(s) 456, 655, 1183, 1381, 1543, 3175
BssNAI GTATAC 1 cut(s) 1049
BssSI CACGAG 1 cut(s) 2039
BssT1I CCWWGG 4 cut(s) 456, 655, 1183, 3175
Bst1107I GTATAC 1 cut(s) 1049
Bst2BI CACGAG 1 cut(s) 2039
Bst2UI CCWGG 4 cut(s) 148, 250, 837, 1168
Bst6I CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
BstAFI CTTAAG 1 cut(s) 710
BstC8I GCNNGC 5 cut(s) 589, 822, 1206, 2160, 3345
BstF5I GGATG 8 cut(s) 258, 396, 466, 903, 1412, 2211, 3451, 3463
BstMWI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
BstNI CCWGG 4 cut(s) 148, 250, 837, 1168
BstNSI RCATGY 1 cut(s) 3313
BstSCI CCNGG 5 cut(s) 146, 248, 835, 1166, 3081
BstSFI CTRYAG 6 cut(s) 428, 669, 1430, 1717, 2433, 3417
BstSLI GKGCMC 1 cut(s) 1841
BstV1I GCAGC 5 cut(s) 118, 677, 708, 1758, 2630
BstV2I GAAGAC 1 cut(s) 3491
BstX2I RGATCY 3 cut(s) 1234, 1885, 2008
BstXI CCANNNNNNTGG 3 cut(s) 177, 533, 824
BstYI RGATCY 3 cut(s) 1234, 1885, 2008
BstZ17I GTATAC 1 cut(s) 1049
Bsu15I ATCGAT 1 cut(s) 2456
BsuI GTATCC 3 cut(s) 2132, 2584, 2816
BsuTUI ATCGAT 1 cut(s) 2456
BtrI CACGTC 1 cut(s) 2854
BtsCI GGATG 8 cut(s) 258, 396, 466, 903, 1412, 2211, 3451, 3463
BtsI GCAGTG 3 cut(s) 437, 2382, 3414
BtsIMutI CAGTG 8 cut(s) 437, 440, 1261, 1269, 1706, 2137, 2382, 3414
BveI ACCTGC 2 cut(s) 2091, 2780
Cac8I GCNNGC 5 cut(s) 589, 822, 1206, 2160, 3345
CaiI CAGNNNCTG 2 cut(s) 1628, 2187
CciI TCATGA 1 cut(s) 2644
Cfr13I GGNCC 4 cut(s) 1837, 1838, 2702, 2884
ClaI ATCGAT 1 cut(s) 2456
CsiI ACCWGGT 1 cut(s) 248
Csp6I GTAC 7 cut(s) 517, 1116, 1359, 1992, 2599, 2609, 2803
CspCI CAANNNNNGTGG 4 cut(s) 413, 448, 981, 1016
CviQI GTAC 7 cut(s) 517, 1116, 1359, 1992, 2599, 2609, 2803
DraI TTTAAA 2 cut(s) 1080, 3270
DrdI GACNNNNNNGTC 1 cut(s) 1943
DseDI GACNNNNNNGTC 1 cut(s) 1943
EaeI YGGCCR 3 cut(s) 1206, 2390, 3172
Eam1104I CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
EarI CTCTTC 5 cut(s) 124, 862, 2564, 2724, 3066
Ecl136II GAGCTC 1 cut(s) 1540
Eco130I CCWWGG 4 cut(s) 456, 655, 1183, 3175
Eco24I GRGCYC 4 cut(s) 571, 1542, 1800, 1841
Eco31I GGTCTC 2 cut(s) 89, 2415
Eco32I GATATC 3 cut(s) 630, 1038, 2108
Eco47I GGWCC 1 cut(s) 2884
Eco53kI GAGCTC 1 cut(s) 1540
Eco57I CTGAAG 1 cut(s) 219
Eco88I CYCGRG 1 cut(s) 3426
EcoICRI GAGCTC 1 cut(s) 1540
EcoO109I RGGNCCY 2 cut(s) 1838, 2884
EcoRI GAATTC 4 cut(s) 445, 1419, 1825, 2978
EcoRII CCWGG 4 cut(s) 146, 248, 835, 1166
EcoRV GATATC 3 cut(s) 630, 1038, 2108
EcoT14I CCWWGG 4 cut(s) 456, 655, 1183, 3175
EcoT22I ATGCAT 1 cut(s) 2051
EcoT38I GRGCYC 4 cut(s) 571, 1542, 1800, 1841
ErhI CCWWGG 4 cut(s) 456, 655, 1183, 3175
Esp3I CGTCTC 2 cut(s) 67, 1951
FalI AAGNNNNNCTT 6 cut(s) 3024, 3056, 3425, 3457, 3476, 3508
FaqI GGGAC 2 cut(s) 657, 3034
FauI CCCGC 1 cut(s) 584
FbaI TGATCA 3 cut(s) 2929, 2995, 3411
FblI GTMKAC 2 cut(s) 1048, 1981
Fnu4HI GCNGC 5 cut(s) 132, 666, 697, 1747, 2619
FokI GGATG 8 cut(s) 245, 383, 473, 890, 1399, 2218, 3458, 3470
FriOI GRGCYC 4 cut(s) 571, 1542, 1800, 1841
Fsp4HI GCNGC 5 cut(s) 132, 666, 697, 1747, 2619
FspBI CTAG 6 cut(s) 917, 1448, 1511, 2486, 2630, 2879
GluI GCNGC 5 cut(s) 132, 666, 697, 1747, 2619
GsaI CCCAGC 1 cut(s) 1928
GsuI CTGGAG 2 cut(s) 2742, 2796
HapII CCGG 2 cut(s) 1416, 3083
HincII GTYRAC 2 cut(s) 6, 3325
HindII GTYRAC 2 cut(s) 6, 3325
HindIII AAGCTT 3 cut(s) 1441, 1852, 2261
HpaII CCGG 2 cut(s) 1416, 3083
Hpy166II GTNNAC 5 cut(s) 6, 842, 1049, 1982, 3325
Hpy8I GTNNAC 5 cut(s) 6, 842, 1049, 1982, 3325
HpyAV CCTTC 7 cut(s) 121, 1494, 1566, 1610, 2135, 2314, 2890
HpyCH4IV ACGT 2 cut(s) 2853, 3438
HpyF10VI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
HpySE526I ACGT 2 cut(s) 2853, 3438
Kpn2I TCCGGA 1 cut(s) 1415
Ksp22I TGATCA 3 cut(s) 2929, 2995, 3411
LguI GCTCTTC 1 cut(s) 3066
Lsp1109I GCAGC 5 cut(s) 118, 677, 708, 1758, 2630
LweI GCATC 2 cut(s) 912, 2058
MabI ACCWGGT 1 cut(s) 248
MaeI CTAG 6 cut(s) 917, 1448, 1511, 2486, 2630, 2879
MaeII ACGT 2 cut(s) 2853, 3438
MfeI CAATTG 2 cut(s) 336, 2988
MflI RGATCY 3 cut(s) 1234, 1885, 2008
MhlI GDGCHC 6 cut(s) 571, 1542, 1671, 1800, 1841, 2877
MlsI TGGCCA 1 cut(s) 1208
MluNI TGGCCA 1 cut(s) 1208
MlyI GAGTC 4 cut(s) 2193, 2297, 2347, 3493
MmeI TCCRAC 5 cut(s) 179, 1364, 1716, 2381, 2802
Mox20I TGGCCA 1 cut(s) 1208
Mph1103I ATGCAT 1 cut(s) 2051
MroI TCCGGA 1 cut(s) 1415
MroXI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
MscI TGGCCA 1 cut(s) 1208
MslI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
Msp20I TGGCCA 1 cut(s) 1208
MspA1I CMGCKG 2 cut(s) 131, 318
MspCI CTTAAG 1 cut(s) 710
MspI CCGG 2 cut(s) 1416, 3083
MspR9I CCNGG 5 cut(s) 148, 250, 837, 1168, 3083
MunI CAATTG 2 cut(s) 336, 2988
Mva1269I GAATGC 1 cut(s) 2768
MvaI CCWGG 4 cut(s) 148, 250, 837, 1168
MwoI GCNNNNNNNGC 4 cut(s) 65, 100, 1177, 2168
NciI CCSGG 1 cut(s) 3083
NlaIV GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1839, 1840, 2901
NmeAIII GCCGAG 2 cut(s) 1293, 3150
NmuCI GTSAC 5 cut(s) 1704, 2287, 2849, 2938, 3184
NsiI ATGCAT 1 cut(s) 2051
NspI RCATGY 1 cut(s) 3313
PaeR7I CTCGAG 1 cut(s) 3426
PagI TCATGA 1 cut(s) 2644
PciSI GCTCTTC 1 cut(s) 3066
PctI GAATGC 1 cut(s) 2768
PdmI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
PflMI CCANNNNNTGG 2 cut(s) 773, 2819
PfoI TCCNGGA 1 cut(s) 146
PkrI GCNGC 5 cut(s) 133, 667, 698, 1748, 2620
PleI GAGTC 4 cut(s) 2192, 2297, 2347, 3493
PpsI GAGTC 4 cut(s) 2192, 2297, 2347, 3493
PpuMI RGGWCCY 1 cut(s) 2884
Psp124BI GAGCTC 1 cut(s) 1542
Psp5II RGGWCCY 1 cut(s) 2884
Psp6I CCWGG 4 cut(s) 146, 248, 835, 1166
PspFI CCCAGC 1 cut(s) 1924
PspGI CCWGG 4 cut(s) 146, 248, 835, 1166
PspN4I GGNNCC 7 cut(s) 254, 978, 1427, 1693, 1839, 1840, 2901
PspOMI GGGCCC 1 cut(s) 1837
PspPI GGNCC 4 cut(s) 1837, 1838, 2702, 2884
PspPPI RGGWCCY 1 cut(s) 2884
PstI CTGCAG 3 cut(s) 432, 1434, 3421
PstNI CAGNNNCTG 2 cut(s) 1628, 2187
PsuI RGATCY 3 cut(s) 1234, 1885, 2008
PvuII CAGCTG 1 cut(s) 131
RsaI GTAC 7 cut(s) 518, 1117, 1360, 1993, 2600, 2610, 2804
RsaNI GTAC 7 cut(s) 517, 1116, 1359, 1992, 2599, 2609, 2803
RseI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
SacI GAGCTC 1 cut(s) 1542
SapI GCTCTTC 1 cut(s) 3066
SatI GCNGC 5 cut(s) 132, 666, 697, 1747, 2619
Sau96I GGNCC 4 cut(s) 1837, 1838, 2702, 2884
ScaI AGTACT 1 cut(s) 1993
SchI GAGTC 4 cut(s) 2193, 2297, 2347, 3493
ScrFI CCNGG 5 cut(s) 148, 250, 837, 1168, 3083
SduI GDGCHC 6 cut(s) 571, 1542, 1671, 1800, 1841, 2877
SexAI ACCWGGT 1 cut(s) 248
SfaNI GCATC 2 cut(s) 912, 2058
SfcI CTRYAG 6 cut(s) 428, 669, 1430, 1717, 2433, 3417
Sfr274I CTCGAG 1 cut(s) 3426
SinI GGWCC 1 cut(s) 2884
SlaI CTCGAG 1 cut(s) 3426
SmiMI CAYNNNNRTG 5 cut(s) 175, 1533, 2375, 2649, 3141
SmlI CTYRAG 3 cut(s) 710, 1286, 3426
SmoI CTYRAG 3 cut(s) 710, 1286, 3426
SsiI CCGC 4 cut(s) 318, 591, 1052, 1372
SspI AATATT 1 cut(s) 1348
SspMI CTAG 6 cut(s) 917, 1448, 1511, 2486, 2630, 2879
SstI GAGCTC 1 cut(s) 1542
StyD4I CCNGG 5 cut(s) 146, 248, 835, 1166, 3081
StyI CCWWGG 4 cut(s) 456, 655, 1183, 3175
TaiI ACGT 2 cut(s) 2856, 3441
TaqI TCGA 6 cut(s) 945, 1800, 2306, 2456, 2671, 3427
TatI WGTACW 2 cut(s) 1991, 2608
TscAI CASTG 8 cut(s) 437, 447, 1261, 1269, 1713, 2137, 2382, 3421
TseFI GTSAC 5 cut(s) 1704, 2287, 2849, 2938, 3184
TseI GCWGC 5 cut(s) 131, 665, 696, 1746, 2618
Tsp45I GTSAC 5 cut(s) 1704, 2287, 2849, 2938, 3184
TspGWI ACGGA 2 cut(s) 572, 2070
TspRI CASTG 8 cut(s) 437, 447, 1261, 1269, 1713, 2137, 2382, 3421
Van91I CCANNNNNTGG 2 cut(s) 773, 2819
Vha464I CTTAAG 1 cut(s) 710
VpaK11BI GGWCC 1 cut(s) 2884
XceI RCATGY 1 cut(s) 3313
XcmI CCANNNNNNNNNTGG 1 cut(s) 1574
XhoI CTCGAG 1 cut(s) 3426
XmiI GTMKAC 2 cut(s) 1048, 1981
XmnI GAANNNNTTC 4 cut(s) 490, 1069, 2718, 3039
XspI CTAG 6 cut(s) 917, 1448, 1511, 2486, 2630, 2879
ZrmI AGTACT 1 cut(s) 1993
Zsp2I ATGCAT 1 cut(s) 2051
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.