Rmu_sc0004503.1_g000004

purine permease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004503.1
OG0018716
Physical Location & Seq
Reverse (-)
18567 .. 21427
2861 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1335 bp
atggcagaagctcagcaagaagttcagcttggcataactcaagataacaaagagggaaactcaactgagctaacgagtgccaaccacgaaccaacaatccatcatcgaagaaattacaagctgtggtttcgaatagcaacctatatattctttgccctctccggccaatcaacaggaacacttctgggaagattctacttcgagaaaggtggaaagagcaaatgggtgggggctctggtgcaactcgcaggatttccaatcctccttcctttctatttcttcgtatcctccaaaattagtcccactccaagtactactactactgctgctgctgctgcttgtcctatccaatattcaaaacccaagtctttgttaacaaccctagcattggtttatgttaccttagggctactcatagcaggaagctgcttcttgacttcctttgggcttcaataccttcctgtctctacgttctcactcatttgtgcttctcaattgggattcacagctttcttttcctttttcctaaactcccaaaagttcactccatttgtgattaattccattgtgctactcaccgtttcctccattctccttgtgttcaataccgactcatcatcagtattagtctctaaagctaagtacgaagtaggattcatatgcaccattggcgctgctgctgggaccggattaggacttgccttaacccagttcgccttcacgaaaataatcaacaagcaaacatttagagcagtggtggatttgataatctatgagtcactaatagcaacatgtgctactttggtcggactttttgctagtggagagtggaagggcttaaatctagagatggatgagtttcaaacaggaaagggatcttatgtgttgactctagcttgggcaatgatttcttggcaagtttttgttattggtgcagtgggattgatatttgaggtttcttccctcttctgcaatgttataagtgctttgggtttgcctgtggttcaagttttggctgtgattgttttccatgacaggatgcatggtttgaagattgtggcaatagttttggccctctggggggtcatggatattcgaaacaagctgacctattcagaaggtatcccgtttcgcagtcactctcagttcgtcaccgtgcctccagcgtcgggcctccgtgagcggcgctcctccgcttcgctgtgccaccacgactctgcctcagtctcccactgttccacagcgcccctccactcaagtagcaaagcagaaagccagaaactgttggttacactcggggttcgatttctgggtttcacttttcaagcttggtaa

Protein Analysis

444

Amino Acids

48.28

Weight (kDa)

9.12

Isoelectric Point (pI)

35.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PUNUT PF16913 45 - 363 1.5e-108 Purine nucleobase transmembrane transport
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018716)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G18190
rosa_chinensis RchiOBHm_Chr7g0210311
rosa_laevigata RLG00000003050
rosa_multiflora Rmu_sc0004503.1_g000004
rosa_roxburghii Rroxscaffold_3G00248440
rosa_samantha Rh7AG253500 Rh7CG270400 Rh7DG261100
rosa_wichuraiana Rw7G021610

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 980
AccBSI CCGCTC 1 cut(s) 1183
AciI CCGC 2 cut(s) 1183, 1194
AclWI GGATC 1 cut(s) 883
AcoI YGGCCR 1 cut(s) 163
AfaI GTAC 2 cut(s) 313, 644
AfiI CCNNNNNNNGG 4 cut(s) 388, 1080, 1081, 1169
AflIII ACRYGT 1 cut(s) 791
AgsI TTSAA 7 cut(s) 357, 452, 604, 863, 1007, 1051, 1325
AjuI GAANNNNNNNTTGG 2 cut(s) 12, 44
AloI GAACNNNNNNTCC 2 cut(s) 81, 113
Alw26I GTCTC 3 cut(s) 469, 634, 1231
AlwI GGATC 1 cut(s) 883
AlwNI CAGNNNCTG 1 cut(s) 1282
Ama87I CYCGRG 1 cut(s) 1295
AoxI GGCC 3 cut(s) 163, 1071, 1171
ApeKI GCWGC 7 cut(s) 326, 329, 332, 335, 426, 674, 677
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AseI ATTAAT 1 cut(s) 558
AspLEI GCGC 3 cut(s) 674, 1188, 1246
AspS9I GGNCC 3 cut(s) 684, 1072, 1171
AsuHPI GGTGA 2 cut(s) 568, 1144
AsuII TTCGAA 2 cut(s) 130, 1096
AvaI CYCGRG 1 cut(s) 1295
AvaII GGWCC 1 cut(s) 684
AxyI CCTNAGG 1 cut(s) 403
BanII GRGCYC 1 cut(s) 235
BbvI GCAGC 7 cut(s) 313, 316, 319, 322, 413, 661, 664
BccI CCATC 2 cut(s) 108, 844
BciVI GTATCC 2 cut(s) 295, 1133
BcoDI GTCTC 3 cut(s) 469, 634, 1231
BfaI CTAG 4 cut(s) 383, 819, 845, 893
BfoI RGCGCY 3 cut(s) 675, 1189, 1247
BfuI GTATCC 2 cut(s) 295, 1133
BisI GCNGC 8 cut(s) 327, 330, 333, 336, 427, 675, 678, 1184
BlpI GCTNAGC 1 cut(s) 12
BlsI GCNGC 8 cut(s) 328, 331, 334, 337, 428, 676, 679, 1185
BmcAI AGTACT 1 cut(s) 313
Bme18I GGWCC 1 cut(s) 684
BmeT110I CYCGRG 1 cut(s) 1295
BmgT120I GGNCC 3 cut(s) 684, 1072, 1171
BmiI GGNNCC 1 cut(s) 685
BmrI ACTGGG 1 cut(s) 703
BmsI GCATC 1 cut(s) 1029
BmuI ACTGGG 1 cut(s) 703
BplI GAGNNNNNCTC 4 cut(s) 44, 76, 1172, 1204
BpmI CTGGAG 1 cut(s) 1146
Bpu1102I GCTNAGC 1 cut(s) 12
Bpu14I TTCGAA 2 cut(s) 130, 1096
BpuEI CTTGAG 2 cut(s) 24, 1240
BsaWI WCCGGW 1 cut(s) 686
BsaXI ACNNNNNCTCC 2 cut(s) 1144, 1174
Bsc4I CCNNNNNNNGG 4 cut(s) 388, 1080, 1081, 1169
Bse1I ACTGG 1 cut(s) 709
Bse21I CCTNAGG 1 cut(s) 403
Bse3DI GCAATG 2 cut(s) 909, 979
BseGI GGATG 2 cut(s) 859, 1044
BseLI CCNNNNNNNGG 4 cut(s) 388, 1080, 1081, 1169
BseMI GCAATG 2 cut(s) 909, 979
BseMII CTCAG 4 cut(s) 26, 57, 1157, 1236
BseNI ACTGG 1 cut(s) 709
BseRI GAGGAG 1 cut(s) 1180
BseXI GCAGC 7 cut(s) 313, 316, 319, 322, 413, 661, 664
BseYI CCCAGC 1 cut(s) 680
BsgI GTGCAG 1 cut(s) 954
BshFI GGCC 3 cut(s) 165, 1073, 1173
BsiHKCI CYCGRG 1 cut(s) 1295
BsiSI CCGG 2 cut(s) 162, 687
BslFI GGGAC 2 cut(s) 285, 697
BslI CCNNNNNNNGG 4 cut(s) 388, 1080, 1081, 1169
BsmAI GTCTC 3 cut(s) 469, 634, 1231
BsmFI GGGAC 2 cut(s) 285, 697
BsnI GGCC 3 cut(s) 165, 1073, 1173
BsoBI CYCGRG 1 cut(s) 1295
Bsp119I TTCGAA 2 cut(s) 130, 1096
Bsp1286I GDGCHC 1 cut(s) 235
Bsp143I GATC 1 cut(s) 875
Bsp1720I GCTNAGC 1 cut(s) 12
BspACI CCGC 2 cut(s) 1183, 1194
BspANI GGCC 3 cut(s) 165, 1073, 1173
BspCNI CTCAG 4 cut(s) 25, 58, 1156, 1235
BspLI GGNNCC 1 cut(s) 685
BspPI GGATC 1 cut(s) 883
BspT104I TTCGAA 2 cut(s) 130, 1096
BsrBI CCGCTC 1 cut(s) 1183
BsrDI GCAATG 2 cut(s) 909, 979
BsrI ACTGG 1 cut(s) 709
BssMI GATC 1 cut(s) 875
Bst4CI ACNGT 4 cut(s) 580, 1156, 1235, 1284
Bst6I CTCTTC 1 cut(s) 971
BstAPI GCANNNNNTGC 1 cut(s) 794
BstBI TTCGAA 2 cut(s) 130, 1096
BstDEI CTNAG 6 cut(s) 12, 66, 403, 639, 1143, 1222
BstF5I GGATG 2 cut(s) 859, 1044
BstH2I RGCGCY 3 cut(s) 675, 1189, 1247
BstHHI GCGC 3 cut(s) 674, 1188, 1246
BstKTI GATC 1 cut(s) 878
BstMAI GTCTC 3 cut(s) 469, 634, 1231
BstMBI GATC 1 cut(s) 875
BstMWI GCNNNNNNNGC 4 cut(s) 332, 335, 669, 794
BstNSI RCATGY 1 cut(s) 795
BstV1I GCAGC 7 cut(s) 313, 316, 319, 322, 413, 661, 664
BstX2I RGATCY 1 cut(s) 875
BstYI RGATCY 1 cut(s) 875
Bsu36I CCTNAGG 1 cut(s) 403
BsuI GTATCC 2 cut(s) 295, 1133
BsuRI GGCC 3 cut(s) 165, 1073, 1173
BtsCI GGATG 2 cut(s) 859, 1044
BtsI GCAGTG 2 cut(s) 759, 942
BtsIMutI CAGTG 3 cut(s) 759, 942, 1231
CaiI CAGNNNCTG 1 cut(s) 1282
CfoI GCGC 3 cut(s) 674, 1188, 1246
Cfr13I GGNCC 3 cut(s) 684, 1072, 1171
CseI GACGC 1 cut(s) 1155
Csp6I GTAC 2 cut(s) 312, 643
CviAII CATG 4 cut(s) 792, 1031, 1043, 1087
CviQI GTAC 2 cut(s) 312, 643
DdeI CTNAG 6 cut(s) 12, 66, 403, 639, 1143, 1222
DpnI GATC 1 cut(s) 877
DpnII GATC 1 cut(s) 875
EaeI YGGCCR 1 cut(s) 163
Eam1104I CTCTTC 1 cut(s) 971
EarI CTCTTC 1 cut(s) 971
Eco24I GRGCYC 1 cut(s) 235
Eco47I GGWCC 1 cut(s) 684
Eco81I CCTNAGG 1 cut(s) 403
Eco88I CYCGRG 1 cut(s) 1295
EcoT22I ATGCAT 1 cut(s) 1044
EcoT38I GRGCYC 1 cut(s) 235
FaeI CATG 4 cut(s) 795, 1034, 1046, 1090
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FaqI GGGAC 2 cut(s) 285, 697
FatI CATG 4 cut(s) 791, 1030, 1042, 1086
FauNDI CATATG 1 cut(s) 659
Fnu4HI GCNGC 8 cut(s) 327, 330, 333, 336, 427, 675, 678, 1184
FokI GGATG 2 cut(s) 866, 1051
FriOI GRGCYC 1 cut(s) 235
Fsp4HI GCNGC 8 cut(s) 327, 330, 333, 336, 427, 675, 678, 1184
FspBI CTAG 4 cut(s) 383, 819, 845, 893
GlaI GCGC 3 cut(s) 673, 1187, 1245
GluI GCNGC 8 cut(s) 327, 330, 333, 336, 427, 675, 678, 1184
GsaI CCCAGC 1 cut(s) 684
GsuI CTGGAG 1 cut(s) 1146
HaeII RGCGCY 3 cut(s) 675, 1189, 1247
HaeIII GGCC 3 cut(s) 165, 1073, 1173
HapII CCGG 2 cut(s) 162, 687
HgaI GACGC 1 cut(s) 1155
HhaI GCGC 3 cut(s) 674, 1188, 1246
Hin1II CATG 4 cut(s) 795, 1034, 1046, 1090
Hin6I GCGC 3 cut(s) 672, 1186, 1244
HinP1I GCGC 3 cut(s) 672, 1186, 1244
HincII GTYRAC 2 cut(s) 375, 888
HindII GTYRAC 2 cut(s) 375, 888
HindIII AAGCTT 1 cut(s) 1326
HinfI GANTC 7 cut(s) 192, 501, 611, 654, 776, 889, 1214
HpaI GTTAAC 1 cut(s) 375
HpaII CCGG 2 cut(s) 162, 687
HphI GGTGA 2 cut(s) 568, 1144
Hpy166II GTNNAC 3 cut(s) 375, 543, 888
Hpy188I TCNGA 2 cut(s) 809, 1117
Hpy188III TCNNGA 5 cut(s) 41, 202, 433, 721, 845
Hpy8I GTNNAC 3 cut(s) 375, 543, 888
Hpy99I CGWCG 1 cut(s) 1171
HpyAV CCTTC 5 cut(s) 275, 467, 727, 826, 1112
HpyCH4III ACNGT 4 cut(s) 580, 1156, 1235, 1284
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 5 cut(s) 241, 663, 935, 972, 1042
HpyF10VI GCNNNNNNNGC 4 cut(s) 332, 335, 669, 794
HpyF3I CTNAG 6 cut(s) 12, 66, 403, 639, 1143, 1222
HpySE526I ACGT 1 cut(s) 470
Hsp92II CATG 4 cut(s) 795, 1034, 1046, 1090
HspAI GCGC 3 cut(s) 672, 1186, 1244
KspAI GTTAAC 1 cut(s) 375
Kzo9I GATC 1 cut(s) 875
LmnI GCTCC 1 cut(s) 1193
Lsp1109I GCAGC 7 cut(s) 313, 316, 319, 322, 413, 661, 664
LweI GCATC 1 cut(s) 1029
MaeI CTAG 4 cut(s) 383, 819, 845, 893
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 5 cut(s) 397, 777, 1136, 1150, 1288
MalI GATC 1 cut(s) 877
MbiI CCGCTC 1 cut(s) 1183
MboI GATC 1 cut(s) 875
MboII GAAGA 6 cut(s) 120, 201, 271, 951, 958, 1063
MfeI CAATTG 1 cut(s) 494
MflI RGATCY 1 cut(s) 875
MhlI GDGCHC 1 cut(s) 235
MluCI AATT 4 cut(s) 112, 294, 494, 559
MlyI GAGTC 4 cut(s) 605, 785, 883, 1208
MmeI TCCRAC 1 cut(s) 787
Mph1103I ATGCAT 1 cut(s) 1044
MseI TTAA 4 cut(s) 374, 558, 704, 839
MspI CCGG 2 cut(s) 162, 687
MunI CAATTG 1 cut(s) 494
MwoI GCNNNNNNNGC 4 cut(s) 332, 335, 669, 794
NdeI CATATG 1 cut(s) 659
NdeII GATC 1 cut(s) 875
NlaIII CATG 4 cut(s) 795, 1034, 1046, 1090
NlaIV GGNNCC 1 cut(s) 685
NmuCI GTSAC 3 cut(s) 777, 1136, 1150
NsiI ATGCAT 1 cut(s) 1044
NspI RCATGY 1 cut(s) 795
NspV TTCGAA 2 cut(s) 130, 1096
PciI ACATGT 1 cut(s) 791
PfeI GAWTC 3 cut(s) 192, 501, 654
PkrI GCNGC 8 cut(s) 328, 331, 334, 337, 428, 676, 679, 1185
PleI GAGTC 4 cut(s) 605, 784, 883, 1208
PpsI GAGTC 4 cut(s) 605, 784, 883, 1208
PscI ACATGT 1 cut(s) 791
PshBI ATTAAT 1 cut(s) 558
PsiI TTATAA 1 cut(s) 980
PspFI CCCAGC 1 cut(s) 680
PspN4I GGNNCC 1 cut(s) 685
PspPI GGNCC 3 cut(s) 684, 1072, 1171
PstNI CAGNNNCTG 1 cut(s) 1282
PsuI RGATCY 1 cut(s) 875
RsaI GTAC 2 cut(s) 313, 644
RsaNI GTAC 2 cut(s) 312, 643
SaqAI TTAA 4 cut(s) 374, 558, 704, 839
SatI GCNGC 8 cut(s) 327, 330, 333, 336, 427, 675, 678, 1184
Sau3AI GATC 1 cut(s) 875
Sau96I GGNCC 3 cut(s) 684, 1072, 1171
ScaI AGTACT 1 cut(s) 313
SchI GAGTC 4 cut(s) 605, 785, 883, 1208
SduI GDGCHC 1 cut(s) 235
SfaNI GCATC 1 cut(s) 1029
SfuI TTCGAA 2 cut(s) 130, 1096
SinI GGWCC 1 cut(s) 684
SmlI CTYRAG 2 cut(s) 39, 1255
SmoI CTYRAG 2 cut(s) 39, 1255
Sse9I AATT 4 cut(s) 112, 294, 494, 559
SsiI CCGC 2 cut(s) 1183, 1194
SspI AATATT 1 cut(s) 353
SspMI CTAG 4 cut(s) 383, 819, 845, 893
TaaI ACNGT 4 cut(s) 580, 1156, 1235, 1284
TaiI ACGT 1 cut(s) 473
TaqI TCGA 5 cut(s) 106, 130, 201, 1096, 1303
TasI AATT 4 cut(s) 112, 294, 494, 559
TatI WGTACW 1 cut(s) 311
TauI GCSGC 1 cut(s) 1186
TfiI GAWTC 3 cut(s) 192, 501, 654
Tru1I TTAA 4 cut(s) 374, 558, 704, 839
Tru9I TTAA 4 cut(s) 374, 558, 704, 839
TscAI CASTG 3 cut(s) 759, 942, 1238
TseFI GTSAC 3 cut(s) 777, 1136, 1150
TseI GCWGC 7 cut(s) 326, 329, 332, 335, 426, 674, 677
Tsp45I GTSAC 3 cut(s) 777, 1136, 1150
TspDTI ATGAA 1 cut(s) 646
TspGWI ACGGA 1 cut(s) 1166
TspRI CASTG 3 cut(s) 759, 942, 1238
VpaK11BI GGWCC 1 cut(s) 684
VspI ATTAAT 1 cut(s) 558
XbaI TCTAGA 1 cut(s) 844
XceI RCATGY 1 cut(s) 795
XspI CTAG 4 cut(s) 383, 819, 845, 893
ZrmI AGTACT 1 cut(s) 313
Zsp2I ATGCAT 1 cut(s) 1044
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.