Rmu_sc0004574.1_g000050

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004574.1
OG0005125
Physical Location & Seq
Forward (+)
148902 .. 153930
5029 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
atgtgctttgattcccagcgtctggagcttctgatgctatcttggcgcagcgggtggcggctcgtcagcggtgatctgggggcgatccggggctcaggcggtagcccgtctggctgtgctctcgcaggtcactcctttggtgagagttggtacctctggcggtacaatgaccatcaacatctccaatattgttacaacaatcaacccctttcatctctgcttcaacatattacaggtaccatctacctcccaaacatggggaatggcgaatcaacgtataatgattctcatgataatttatggacggatgatgattctcatgatagtttcccacctgaaccaccttcctatgcaggcacttcgatggactctagttctaaacaaaagctaccatctacacgtataggcgacaatttcaactctttggatcaggttattgcttcattgagagaagctggccttgaatcatcaaatttaatacttggtatcgacttcacaaagagcaatgaatggacaggcaaatattcattcaacagaaaaagtcttcatgctatcggtagcacacctaatccttacgagcaagcaatctctataattggccgcactttgtctcctttcgatgaagataatataattccttgttttggatttggcgatgcatcaacacatgatcaaggtgtgttccgcttttatcctgatgatcgatgctgtgttgggttcgaggaagctcttgctcggtatagggagattgttccattcttaaggttatcaggtccaacctcgttttccccaataattgatgcggcaattgacattgttgaaaaaaacaatggacaataccatgttcttgttattatagcagacggacaggttaccagaagccctgataccccacctggaagattcagtgcccaagaacaagcgactgtaaattccatagttgctgctagcaaatatcctctgtcaattatcttggttggagttggggacggaccgtgggatgcaatgcagcaatttgatgataacattcctcaacaagcatttgacaacttctag

Protein Analysis

351

Amino Acids

38.82

Weight (kDa)

4.69

Isoelectric Point (pI)

40.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Copine PF07002 181 - 342 1.8e-58 Copine
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 116
Acc65I GGTACC 2 cut(s) 150, 236
AccB1I GGYRCC 2 cut(s) 150, 236
AccB7I CCANNNNNTGG 1 cut(s) 22
AciI CCGC 8 cut(s) 51, 58, 69, 99, 160, 601, 685, 803
AclWI GGATC 2 cut(s) 79, 435
AcoI YGGCCR 1 cut(s) 598
AcsI RAATTY 2 cut(s) 472, 931
AfaI GTAC 3 cut(s) 152, 164, 238
AfiI CCNNNNNNNGG 4 cut(s) 22, 256, 257, 644
AflII CTTAAG 1 cut(s) 760
AflIII ACRYGT 1 cut(s) 398
AgsI TTSAA 5 cut(s) 224, 418, 464, 532, 821
AjnI CCWGG 1 cut(s) 895
AjuI GAANNNNNNNTTGG 2 cut(s) 769, 801
AluBI AGCT 4 cut(s) 28, 388, 455, 728
AluI AGCT 4 cut(s) 28, 388, 455, 728
Alw21I GWGCWC 1 cut(s) 121
Alw26I GTCTC 1 cut(s) 615
AlwI GGATC 2 cut(s) 79, 435
AlwNI CAGNNNCTG 1 cut(s) 22
AoxI GGCC 2 cut(s) 457, 598
ApeKI GCWGC 3 cut(s) 48, 944, 1009
ApoI RAATTY 2 cut(s) 472, 931
Asp718I GGTACC 2 cut(s) 150, 236
AspLEI GCGC 1 cut(s) 48
AspS9I GGNCC 2 cut(s) 773, 992
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 83, 152
AsuNHI GCTAGC 1 cut(s) 947
AvaII GGWCC 2 cut(s) 773, 992
BaeGI GKGCMC 1 cut(s) 913
BanI GGYRCC 2 cut(s) 150, 236
BanII GRGCYC 1 cut(s) 95
BarI GAAGNNNNNNTAC 2 cut(s) 871, 903
BbsI GAAGAC 1 cut(s) 536
Bbv12I GWGCWC 1 cut(s) 121
BbvI GCAGC 3 cut(s) 60, 931, 1021
BccI CCATC 4 cut(s) 180, 248, 358, 400
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 897
BclI TGATCA 1 cut(s) 670
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 3 cut(s) 372, 948, 1054
BfrI CTTAAG 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 116
BglI GCCNNNNNGGC 1 cut(s) 111
BisI GCNGC 6 cut(s) 49, 59, 601, 804, 945, 1010
BlsI GCNGC 6 cut(s) 50, 60, 602, 805, 946, 1011
Bme1390I CCNGG 2 cut(s) 89, 897
Bme18I GGWCC 2 cut(s) 773, 992
BmgT120I GGNCC 2 cut(s) 773, 992
BmiI GGNNCC 2 cut(s) 152, 238
BmrFI CCNGG 2 cut(s) 89, 897
BmsI GCATC 6 cut(s) 24, 646, 668, 695, 790, 991
BmtI GCTAGC 1 cut(s) 951
BpiI GAAGAC 1 cut(s) 536
BpmI CTGGAG 1 cut(s) 44
Bpu10I CCTNAGC 1 cut(s) 94
BpuMI CCSGG 1 cut(s) 89
Bsa29I ATCGAT 1 cut(s) 703
BsaAI YACGTR 1 cut(s) 401
BsaJI CCNNGG 2 cut(s) 88, 995
BsaXI ACNNNNNCTCC 2 cut(s) 595, 625
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 256, 257, 644
Bse3DI GCAATG 2 cut(s) 511, 1011
BseBI CCWGG 1 cut(s) 897
BseCI ATCGAT 1 cut(s) 703
BseDI CCNNGG 2 cut(s) 88, 995
BseGI GGATG 2 cut(s) 313, 1006
BseLI CCNNNNNNNGG 4 cut(s) 22, 256, 257, 644
BseMI GCAATG 2 cut(s) 511, 1011
BseMII CTCAG 1 cut(s) 108
BseSI GKGCMC 1 cut(s) 913
BseXI GCAGC 3 cut(s) 60, 931, 1021
BseYI CCCAGC 1 cut(s) 15
BshFI GGCC 2 cut(s) 459, 600
BshNI GGYRCC 2 cut(s) 150, 236
BshVI ATCGAT 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 121
BsiSI CCGG 1 cut(s) 88
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 4 cut(s) 22, 256, 257, 644
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 1001
BsnI GGCC 2 cut(s) 459, 600
Bsp1286I GDGCHC 3 cut(s) 95, 121, 913
Bsp143I GATC 5 cut(s) 73, 84, 427, 670, 700
BspACI CCGC 8 cut(s) 51, 58, 69, 99, 160, 601, 685, 803
BspANI GGCC 2 cut(s) 459, 600
BspCNI CTCAG 1 cut(s) 107
BspDI ATCGAT 1 cut(s) 703
BspHI TCATGA 2 cut(s) 289, 319
BspLI GGNNCC 2 cut(s) 152, 238
BspMI ACCTGC 1 cut(s) 116
BspOI GCTAGC 1 cut(s) 951
BspPI GGATC 2 cut(s) 79, 435
BspT107I GGYRCC 2 cut(s) 150, 236
BspTI CTTAAG 1 cut(s) 760
BsrDI GCAATG 2 cut(s) 511, 1011
BssECI CCNNGG 2 cut(s) 88, 995
BssMI GATC 5 cut(s) 73, 84, 427, 670, 700
Bst2UI CCWGG 1 cut(s) 897
Bst4CI ACNGT 2 cut(s) 928, 996
BstAFI CTTAAG 1 cut(s) 760
BstBAI YACGTR 1 cut(s) 401
BstC8I GCNNGC 4 cut(s) 355, 457, 582, 949
BstDEI CTNAG 1 cut(s) 94
BstDSI CCRYGG 1 cut(s) 995
BstEII GGTNACC 1 cut(s) 871
BstF5I GGATG 2 cut(s) 313, 1006
BstHHI GCGC 1 cut(s) 48
BstKTI GATC 5 cut(s) 76, 87, 430, 673, 703
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 5 cut(s) 73, 84, 427, 670, 700
BstMWI GCNNNNNNNGC 4 cut(s) 25, 34, 43, 111
BstNI CCWGG 1 cut(s) 897
BstPI GGTNACC 1 cut(s) 871
BstSCI CCNGG 2 cut(s) 87, 895
BstSLI GKGCMC 1 cut(s) 913
BstV1I GCAGC 3 cut(s) 60, 931, 1021
BstV2I GAAGAC 1 cut(s) 536
Bsu15I ATCGAT 1 cut(s) 703
BsuRI GGCC 2 cut(s) 459, 600
BsuTUI ATCGAT 1 cut(s) 703
BtgI CCRYGG 1 cut(s) 995
BtgZI GCGATG 1 cut(s) 669
BtsCI GGATG 2 cut(s) 313, 1006
BtsIMutI CAGTG 1 cut(s) 913
BveI ACCTGC 1 cut(s) 116
Cac8I GCNNGC 4 cut(s) 355, 457, 582, 949
CaiI CAGNNNCTG 1 cut(s) 22
CciI TCATGA 2 cut(s) 289, 319
CfoI GCGC 1 cut(s) 48
Cfr13I GGNCC 2 cut(s) 773, 992
ClaI ATCGAT 1 cut(s) 703
CpoI CGGWCCG 1 cut(s) 992
CseI GACGC 1 cut(s) 8
Csp6I GTAC 3 cut(s) 151, 163, 237
CspI CGGWCCG 1 cut(s) 992
CviAII CATG 6 cut(s) 256, 290, 320, 548, 668, 842
CviQI GTAC 3 cut(s) 151, 163, 237
DdeI CTNAG 1 cut(s) 94
DpnI GATC 5 cut(s) 75, 86, 429, 672, 702
DpnII GATC 5 cut(s) 73, 84, 427, 670, 700
EaeI YGGCCR 1 cut(s) 598
Eco24I GRGCYC 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 773, 992
Eco91I GGTNACC 1 cut(s) 871
EcoO65I GGTNACC 1 cut(s) 871
EcoRII CCWGG 1 cut(s) 895
EcoT22I ATGCAT 1 cut(s) 661
EcoT38I GRGCYC 1 cut(s) 95
FaeI CATG 6 cut(s) 259, 293, 323, 551, 671, 845
FalI AAGNNNNNCTT 2 cut(s) 444, 476
FaqI GGGAC 1 cut(s) 1001
FatI CATG 6 cut(s) 255, 289, 319, 547, 667, 841
FauI CCCGC 1 cut(s) 44
FbaI TGATCA 1 cut(s) 670
Fnu4HI GCNGC 6 cut(s) 49, 59, 601, 804, 945, 1010
FokI GGATG 2 cut(s) 320, 1013
FriOI GRGCYC 1 cut(s) 95
Fsp4HI GCNGC 6 cut(s) 49, 59, 601, 804, 945, 1010
FspBI CTAG 3 cut(s) 372, 948, 1054
GlaI GCGC 1 cut(s) 47
GluI GCNGC 6 cut(s) 49, 59, 601, 804, 945, 1010
GsaI CCCAGC 1 cut(s) 19
GsuI CTGGAG 1 cut(s) 44
HaeIII GGCC 2 cut(s) 459, 600
HapII CCGG 1 cut(s) 88
HgaI GACGC 1 cut(s) 8
HhaI GCGC 1 cut(s) 48
Hin1II CATG 6 cut(s) 259, 293, 323, 551, 671, 845
Hin6I GCGC 1 cut(s) 46
HinP1I GCGC 1 cut(s) 46
HinfI GANTC 7 cut(s) 11, 269, 284, 314, 368, 464, 903
HpaII CCGG 1 cut(s) 88
HphI GGTGA 2 cut(s) 83, 152
Hpy188I TCNGA 1 cut(s) 33
Hpy188III TCNNGA 4 cut(s) 23, 290, 320, 695
HpyAV CCTTC 1 cut(s) 354
HpyCH4III ACNGT 2 cut(s) 928, 996
HpyCH4IV ACGT 2 cut(s) 275, 400
HpyCH4V TGCA 4 cut(s) 353, 659, 1004, 1009
HpyF10VI GCNNNNNNNGC 4 cut(s) 25, 34, 43, 111
HpyF3I CTNAG 1 cut(s) 94
HpySE526I ACGT 2 cut(s) 275, 400
Hsp92II CATG 6 cut(s) 259, 293, 323, 551, 671, 845
HspAI GCGC 1 cut(s) 46
KpnI GGTACC 2 cut(s) 154, 240
Ksp22I TGATCA 1 cut(s) 670
Kzo9I GATC 5 cut(s) 73, 84, 427, 670, 700
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 3 cut(s) 60, 931, 1021
LweI GCATC 6 cut(s) 24, 646, 668, 695, 790, 991
MaeI CTAG 3 cut(s) 372, 948, 1054
MaeII ACGT 2 cut(s) 275, 400
MaeIII GTNAC 3 cut(s) 128, 191, 871
MalI GATC 5 cut(s) 75, 86, 429, 672, 702
MboI GATC 5 cut(s) 73, 84, 427, 670, 700
MboII GAAGA 3 cut(s) 536, 635, 912
MfeI CAATTG 1 cut(s) 807
MhlI GDGCHC 3 cut(s) 95, 121, 913
MlyI GAGTC 1 cut(s) 362
MmeI TCCRAC 2 cut(s) 800, 958
MnlI CCTC 6 cut(s) 164, 257, 715, 790, 969, 1041
Mph1103I ATGCAT 1 cut(s) 661
MseI TTAA 2 cut(s) 476, 761
MslI CAYNNNNRTG 1 cut(s) 362
MspA1I CMGCKG 2 cut(s) 51, 69
MspCI CTTAAG 1 cut(s) 760
MspI CCGG 1 cut(s) 88
MspR9I CCNGG 2 cut(s) 89, 897
MunI CAATTG 1 cut(s) 807
MvaI CCWGG 1 cut(s) 897
MwoI GCNNNNNNNGC 4 cut(s) 25, 34, 43, 111
NciI CCSGG 1 cut(s) 89
NdeII GATC 5 cut(s) 73, 84, 427, 670, 700
NheI GCTAGC 1 cut(s) 947
NlaIII CATG 6 cut(s) 259, 293, 323, 551, 671, 845
NlaIV GGNNCC 2 cut(s) 152, 238
NmuCI GTSAC 1 cut(s) 128
NsiI ATGCAT 1 cut(s) 661
PagI TCATGA 2 cut(s) 289, 319
PfeI GAWTC 6 cut(s) 11, 269, 284, 314, 464, 903
PflMI CCANNNNNTGG 1 cut(s) 22
PkrI GCNGC 6 cut(s) 50, 60, 602, 805, 946, 1011
PleI GAGTC 1 cut(s) 362
PpsI GAGTC 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 401
Psp6I CCWGG 1 cut(s) 895
PspEI GGTNACC 1 cut(s) 871
PspFI CCCAGC 1 cut(s) 15
PspGI CCWGG 1 cut(s) 895
PspN4I GGNNCC 2 cut(s) 152, 238
PspPI GGNCC 2 cut(s) 773, 992
PstNI CAGNNNCTG 1 cut(s) 22
RsaI GTAC 3 cut(s) 152, 164, 238
RsaNI GTAC 3 cut(s) 151, 163, 237
RseI CAYNNNNRTG 1 cut(s) 362
Rsr2I CGGWCCG 1 cut(s) 992
RsrII CGGWCCG 1 cut(s) 992
SaqAI TTAA 2 cut(s) 476, 761
SatI GCNGC 6 cut(s) 49, 59, 601, 804, 945, 1010
Sau3AI GATC 5 cut(s) 73, 84, 427, 670, 700
Sau96I GGNCC 2 cut(s) 773, 992
SchI GAGTC 1 cut(s) 362
ScrFI CCNGG 2 cut(s) 89, 897
SduI GDGCHC 3 cut(s) 95, 121, 913
SfaNI GCATC 6 cut(s) 24, 646, 668, 695, 790, 991
SinI GGWCC 2 cut(s) 773, 992
SmiMI CAYNNNNRTG 1 cut(s) 362
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
SsiI CCGC 8 cut(s) 51, 58, 69, 99, 160, 601, 685, 803
SspI AATATT 2 cut(s) 188, 524
SspMI CTAG 3 cut(s) 372, 948, 1054
StyD4I CCNGG 2 cut(s) 87, 895
TaaI ACNGT 2 cut(s) 928, 996
TaiI ACGT 2 cut(s) 278, 403
TaqI TCGA 5 cut(s) 362, 489, 618, 703, 720
TauI GCSGC 3 cut(s) 61, 603, 806
TfiI GAWTC 6 cut(s) 11, 269, 284, 314, 464, 903
Tru1I TTAA 2 cut(s) 476, 761
Tru9I TTAA 2 cut(s) 476, 761
TscAI CASTG 1 cut(s) 913
TseFI GTSAC 1 cut(s) 128
TseI GCWGC 3 cut(s) 48, 944, 1009
Tsp45I GTSAC 1 cut(s) 128
TspDTI ATGAA 6 cut(s) 201, 432, 516, 522, 536, 636
TspGWI ACGGA 3 cut(s) 320, 879, 1005
TspRI CASTG 1 cut(s) 913
Van91I CCANNNNNTGG 1 cut(s) 22
Vha464I CTTAAG 1 cut(s) 760
VpaK11BI GGWCC 2 cut(s) 773, 992
XapI RAATTY 2 cut(s) 472, 931
XspI CTAG 3 cut(s) 372, 948, 1054
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.