Rmu_sc0004856.1_g000008

khg kdpg

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004856.1
OG0014787
Physical Location & Seq
Forward (+)
27650 .. 32286
4637 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
atgtacagtgcagagctggcatttgaagctgcttgtgctgctcttgaaggcggcatttcagttcttgagattgtgatgtcgacaccaggtgtgtttgaggttctacagaagctggtacatatctaccctacgagagtcctaggagtagggacagtactgaacattgatgatgcggaaagggcagtgacagctggagccaagttttttatgagtcctgttatagtgaaggatattatggatgattttcggtatggggatgttttgtacatacctggagttatgactccaacagagattttgtctgcctatgatgctggtgcaaaaattgtgaagatttatcctgtttctgcattaggcggcatacactatatatcagccatcaagaaaccttttcctcacatctcaatggttgcttctcaaggcataacaataggttcagctggggaatatattgctaagggagcatcttcagttgtcttatcagatgccatatttgataaagaagcaatgggtcaaaagaatttcaatagaatacatcaactcgcgaacatggcagctttgcagtgcaaggaagcagtagatcggaagaaaaacctcatgctgatcaataatttgtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Aldolase

Protein Analysis

205

Amino Acids

22.03

Weight (kDa)

6.07

Isoelectric Point (pI)

34.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Aldolase PF01081 8 - 181 1.3e-32 KDPG and KHG aldolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014787)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g04260 FvH4_7g04260 FvH4_7g04260
malus_domestica MD02G1272300.v1.1
prunus_persica Prupe.2G048500_v2.0.a1
pyrus_communis pycom02g23290
rosa_chinensis RchiOBHm_Chr1g0328821
rosa_laevigata RLG00000029917
rosa_multiflora Rmu_sc0004856.1_g000008
rosa_roxburghii Rroxscaffold_4G00321360
rosa_rugosa Rorug01G0075900 Rorug01G0075900
rosa_samantha Rh1AG095100 Rh1BG075600 Rh1CG092200 Rh1DG098300
rosa_wichuraiana Rw1G007410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 80
AccII CGCG 1 cut(s) 545
AciI CCGC 3 cut(s) 51, 173, 357
AcsI RAATTY 1 cut(s) 520
AcuI CTGAAG 1 cut(s) 453
AdeI CACNNNGTG 1 cut(s) 89
AfaI GTAC 4 cut(s) 5, 117, 156, 266
AgsI TTSAA 3 cut(s) 26, 47, 526
AjnI CCWGG 2 cut(s) 85, 271
AluBI AGCT 6 cut(s) 16, 29, 112, 191, 440, 557
AluI AGCT 6 cut(s) 16, 29, 112, 191, 440, 557
AlwNI CAGNNNCTG 1 cut(s) 112
ApeKI GCWGC 3 cut(s) 29, 38, 554
ApoI RAATTY 1 cut(s) 520
AspA2I CCTAGG 1 cut(s) 139
AvrII CCTAGG 1 cut(s) 139
BbvI GCAGC 3 cut(s) 16, 25, 566
BccI CCATC 1 cut(s) 386
BciT130I CCWGG 2 cut(s) 87, 273
BclI TGATCA 1 cut(s) 603
BfaI CTAG 1 cut(s) 140
BfmI CTRYAG 1 cut(s) 104
BisI GCNGC 5 cut(s) 30, 39, 52, 358, 555
BlnI CCTAGG 1 cut(s) 139
BlsI GCNGC 5 cut(s) 31, 40, 53, 359, 556
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 2 cut(s) 87, 273
BmiI GGNNCC 1 cut(s) 196
BmrFI CCNGG 2 cut(s) 87, 273
BmsI GCATC 4 cut(s) 160, 301, 473, 475
BpmI CTGGAG 2 cut(s) 213, 294
Bpu10I CCTNAGC 1 cut(s) 456
BpuEI CTTGAG 2 cut(s) 86, 402
BsaJI CCNNGG 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 513
BseBI CCWGG 2 cut(s) 87, 273
BseDI CCNNGG 1 cut(s) 139
BseGI GGATG 2 cut(s) 244, 262
BseMI GCAATG 1 cut(s) 513
BseXI GCAGC 3 cut(s) 16, 25, 566
BseYI CCCAGC 1 cut(s) 440
BsgI GTGCAG 1 cut(s) 30
Bsh1236I CGCG 1 cut(s) 545
BslFI GGGAC 1 cut(s) 163
BsmFI GGGAC 1 cut(s) 163
Bsp1407I TGTACA 2 cut(s) 3, 264
Bsp143I GATC 2 cut(s) 580, 603
Bsp68I TCGCGA 1 cut(s) 545
BspACI CCGC 3 cut(s) 51, 173, 357
BspFNI CGCG 1 cut(s) 545
BspLI GGNNCC 1 cut(s) 196
BsrDI GCAATG 1 cut(s) 513
BsrGI TGTACA 2 cut(s) 3, 264
BssECI CCNNGG 1 cut(s) 139
BssMI GATC 2 cut(s) 580, 603
BssT1I CCWWGG 1 cut(s) 139
Bst2UI CCWGG 2 cut(s) 87, 273
Bst4CI ACNGT 2 cut(s) 8, 154
BstAUI TGTACA 2 cut(s) 3, 264
BstC8I GCNNGC 1 cut(s) 18
BstDEI CTNAG 1 cut(s) 456
BstF5I GGATG 2 cut(s) 244, 262
BstFNI CGCG 1 cut(s) 545
BstKTI GATC 2 cut(s) 583, 606
BstMBI GATC 2 cut(s) 580, 603
BstMWI GCNNNNNNNGC 9 cut(s) 17, 26, 35, 38, 179, 188, 311, 461, 551
BstNI CCWGG 2 cut(s) 87, 273
BstSCI CCNGG 2 cut(s) 85, 271
BstSFI CTRYAG 1 cut(s) 104
BstUI CGCG 1 cut(s) 545
BstV1I GCAGC 3 cut(s) 16, 25, 566
BtsCI GGATG 2 cut(s) 244, 262
BtsI GCAGTG 2 cut(s) 189, 569
BtsIMutI CAGTG 3 cut(s) 13, 189, 569
BtuMI TCGCGA 1 cut(s) 545
Cac8I GCNNGC 1 cut(s) 18
CaiI CAGNNNCTG 1 cut(s) 112
CsiI ACCWGGT 1 cut(s) 85
Csp6I GTAC 4 cut(s) 4, 116, 155, 265
CviAII CATG 2 cut(s) 550, 598
CviJI RGCY 8 cut(s) 16, 29, 112, 191, 197, 377, 440, 557
CviKI_1 RGCY 8 cut(s) 16, 29, 112, 191, 197, 377, 440, 557
CviQI GTAC 4 cut(s) 4, 116, 155, 265
DdeI CTNAG 1 cut(s) 456
DpnI GATC 2 cut(s) 582, 605
DpnII GATC 2 cut(s) 580, 603
DraIII CACNNNGTG 1 cut(s) 89
Eco130I CCWWGG 1 cut(s) 139
Eco57I CTGAAG 1 cut(s) 453
EcoRII CCWGG 2 cut(s) 85, 271
EcoT14I CCWWGG 1 cut(s) 139
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 2 cut(s) 553, 601
FaqI GGGAC 1 cut(s) 163
FatI CATG 2 cut(s) 549, 597
FbaI TGATCA 1 cut(s) 603
FblI GTMKAC 1 cut(s) 80
Fnu4HI GCNGC 5 cut(s) 30, 39, 52, 358, 555
FokI GGATG 2 cut(s) 251, 269
Fsp4HI GCNGC 5 cut(s) 30, 39, 52, 358, 555
FspBI CTAG 1 cut(s) 140
GluI GCNGC 5 cut(s) 30, 39, 52, 358, 555
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 2 cut(s) 213, 294
Hin1II CATG 2 cut(s) 553, 601
HincII GTYRAC 1 cut(s) 81
HindII GTYRAC 1 cut(s) 81
HinfI GANTC 3 cut(s) 135, 211, 283
Hpy166II GTNNAC 1 cut(s) 81
Hpy188I TCNGA 2 cut(s) 484, 585
Hpy188III TCNNGA 4 cut(s) 44, 65, 382, 544
Hpy8I GTNNAC 1 cut(s) 81
HpyAV CCTTC 2 cut(s) 41, 220
HpyCH4III ACNGT 2 cut(s) 8, 154
HpyCH4V TGCA 5 cut(s) 11, 320, 350, 562, 567
HpyF10VI GCNNNNNNNGC 9 cut(s) 17, 26, 35, 38, 179, 188, 311, 461, 551
HpyF3I CTNAG 1 cut(s) 456
Hsp92II CATG 2 cut(s) 553, 601
Ksp22I TGATCA 1 cut(s) 603
Kzo9I GATC 2 cut(s) 580, 603
LmnI GCTCC 2 cut(s) 194, 461
Lsp1109I GCAGC 3 cut(s) 16, 25, 566
LweI GCATC 4 cut(s) 160, 301, 473, 475
MabI ACCWGGT 1 cut(s) 85
MaeI CTAG 1 cut(s) 140
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 582, 605
MboI GATC 2 cut(s) 580, 603
MboII GAAGA 3 cut(s) 343, 459, 598
MluCI AATT 3 cut(s) 324, 520, 610
MlyI GAGTC 3 cut(s) 144, 220, 277
MmeI TCCRAC 1 cut(s) 311
MnlI CCTC 3 cut(s) 91, 405, 605
MslI CAYNNNNRTG 1 cut(s) 404
MspA1I CMGCKG 2 cut(s) 191, 440
MspR9I CCNGG 2 cut(s) 87, 273
MvaI CCWGG 2 cut(s) 87, 273
MvnI CGCG 1 cut(s) 545
MwoI GCNNNNNNNGC 9 cut(s) 17, 26, 35, 38, 179, 188, 311, 461, 551
NdeII GATC 2 cut(s) 580, 603
NlaIII CATG 2 cut(s) 553, 601
NlaIV GGNNCC 1 cut(s) 196
NmuCI GTSAC 1 cut(s) 184
NruI TCGCGA 1 cut(s) 545
PkrI GCNGC 5 cut(s) 31, 40, 53, 359, 556
PleI GAGTC 3 cut(s) 143, 219, 277
PpsI GAGTC 3 cut(s) 143, 219, 277
Psp6I CCWGG 2 cut(s) 85, 271
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 2 cut(s) 85, 271
PspN4I GGNNCC 1 cut(s) 196
PstNI CAGNNNCTG 1 cut(s) 112
PvuII CAGCTG 2 cut(s) 191, 440
RruI TCGCGA 1 cut(s) 545
RsaI GTAC 4 cut(s) 5, 117, 156, 266
RsaNI GTAC 4 cut(s) 4, 116, 155, 265
RseI CAYNNNNRTG 1 cut(s) 404
SalI GTCGAC 1 cut(s) 79
SatI GCNGC 5 cut(s) 30, 39, 52, 358, 555
Sau3AI GATC 2 cut(s) 580, 603
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 3 cut(s) 144, 220, 277
ScrFI CCNGG 2 cut(s) 87, 273
SexAI ACCWGGT 1 cut(s) 85
SfaNI GCATC 4 cut(s) 160, 301, 473, 475
SfcI CTRYAG 1 cut(s) 104
SmiMI CAYNNNNRTG 1 cut(s) 404
SmlI CTYRAG 2 cut(s) 65, 417
SmoI CTYRAG 2 cut(s) 65, 417
Sse9I AATT 3 cut(s) 324, 520, 610
SsiI CCGC 3 cut(s) 51, 173, 357
SspMI CTAG 1 cut(s) 140
StyD4I CCNGG 2 cut(s) 85, 271
StyI CCWWGG 1 cut(s) 139
TaaI ACNGT 2 cut(s) 8, 154
TaqI TCGA 1 cut(s) 80
TasI AATT 3 cut(s) 324, 520, 610
TatI WGTACW 3 cut(s) 3, 154, 264
TauI GCSGC 2 cut(s) 54, 360
TscAI CASTG 3 cut(s) 13, 189, 569
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 3 cut(s) 29, 38, 554
Tsp45I GTSAC 1 cut(s) 184
TspRI CASTG 3 cut(s) 13, 189, 569
XapI RAATTY 1 cut(s) 520
XmaJI CCTAGG 1 cut(s) 139
XmiI GTMKAC 1 cut(s) 80
XspI CTAG 1 cut(s) 140
ZrmI AGTACT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.