Rmu_sc0004865.1_g000001

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004865.1
OG0017822
Physical Location & Seq
Reverse (-)
2 .. 1730
1729 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004865.1_g000001.1.cds

Sequence Viewer

Length: 1652 bp
atgcttgggagcttggttgaccgcttcattggttgggcgctgccgttgcttagcaatatcagcgccggagatgggaccgcagaattggcattggacggcctgcaagagtttcttaacgttggggatgttggcgggatcgagagatatgctttgtcaattcttaaagcgtgccaagttcttcttgaggatgagagaacctccttgagtctgttgcaccgccttctcggtgttttgactttgatttcattgaagttttctagatacttccaacctcattttcttgacattgttgatctgcttctcgggtgggcgttggtaccggaccttgccgaatcagatagaagaattataatggatagtttcttacagtttcagaatcattgggtgggtagtttgcagttttctctgggattgctgtcgaagtttgtaggagacatggatgttttgattcaggattccactgcttctgggttgctggaaatgaatctacttgaacaaattactgaaccccttagcagaattattcctcggttgttgggatgtttatctatggttggaaggaaatttgggtggttggaatggattggggattcatggaagtgtctgactctgctggcagaaattttttgtgaaagattttccaccttttatgcacttgcggttgatattttatttcaaagtttggaagtggacaatagtaaccaagcagtgggaactggaagaattagctccttccaagttcatggagtattaaaaactaatctccaattattatctttgcagaagtctgggcttctcccaatctctgtgcagaaaatactgcaatttgatgctccaatctctcagttgcgtttgcatcctaatcacttggtaacaggcagttcggctgccacttatatattcttgcttcaacacgggaataatgaagttgttgaacaagcactgacttcgttaacagaggagctggagttattagaagggatgctagagaaatctgtgggccatgatgatggggttcttgcatgttccaaaacatattcaaaacgtgaattgtttgcattgattaaatttgatttgaaggttcttctgtcatgtgttttcttcagtgggggtaatagttatagtttgattggccaaccagacattgcaaccctatatcttatgaagtcagaaaagttggtagagtttattattgagaaatttaatccttttgacttgcctattatggaatatgtggatttgcaagtcagtgttttgaagacgtttgacaggttaacagtagttaaattcttaagtacctgctctttgtcatgtcagagtagtggaaaatcatcaatggatgttacctctgataaattacttaatagtgagtctttgacgaatgaacattcggttgtggttgttgagaatctgagaaagtacagtctgtcttttgtgaaagctctccatgtctcttctcctcttgcagttaaagtaactgcgctggattgggtacaaagattttgtgagaatgttattgcttttaatgagaaatcagacacagaaactcgtttctatgaagtgtatggtaacgatagaattattgggaacatgctttactcaattctggatgctgcatctgatagggaaccaaaagtgaggtcacatgttgc
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006401 GO:0006402 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019439 GO:0019538 GO:0019637 GO:0030258 GO:0031323 GO:0031974 GO:0031981 GO:0032204 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034655 GO:0036211 GO:0042162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046486 GO:0046488 GO:0046700 GO:0046777 GO:0046834 GO:0046854 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140096 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901575 GO:2001020
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
FATC PI3_PI4_kinase SMG1 WD40

Protein Analysis

551

Amino Acids

61.46

Weight (kDa)

5.12

Isoelectric Point (pI)

31.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 350
Acc36I ACCTGC 1 cut(s) 1307
Acc65I GGTACC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 316
AccB7I CCANNNNNTGG 1 cut(s) 709
AciI CCGC 5 cut(s) 22, 78, 132, 217, 659
AclI AACGTT 1 cut(s) 117
AclWI GGATC 1 cut(s) 143
AcoI YGGCCR 1 cut(s) 1132
AcsI RAATTY 5 cut(s) 563, 621, 1067, 1199, 1286
AcuI CTGAAG 1 cut(s) 1087
AfaI GTAC 4 cut(s) 318, 1297, 1421, 1494
AfiI CCNNNNNNNGG 2 cut(s) 72, 709
AflII CTTAAG 1 cut(s) 1291
AflIII ACRYGT 1 cut(s) 1645
AgsI TTSAA 8 cut(s) 250, 494, 677, 911, 935, 1041, 1078, 1258
AjuI GAANNNNNNNTTGG 2 cut(s) 261, 293
AluBI AGCT 4 cut(s) 12, 729, 964, 1442
AluI AGCT 4 cut(s) 12, 729, 964, 1442
Alw26I GTCTC 2 cut(s) 426, 1456
AlwI GGATC 1 cut(s) 143
Ama87I CYCGRG 1 cut(s) 302
AoxI GGCC 3 cut(s) 97, 1000, 1132
ApeKI GCWGC 3 cut(s) 40, 887, 1613
ApoI RAATTY 5 cut(s) 563, 621, 1067, 1199, 1286
ArsI GACNNNNNNTTYG 1 cut(s) 1625
Asp718I GGTACC 1 cut(s) 316
AspLEI GCGC 3 cut(s) 40, 65, 1483
AspS9I GGNCC 3 cut(s) 75, 322, 1000
AvaI CYCGRG 1 cut(s) 302
AvaII GGWCC 2 cut(s) 75, 322
BalI TGGCCA 1 cut(s) 1134
BanI GGYRCC 1 cut(s) 316
BbsI GAAGAC 1 cut(s) 1265
BbvI GCAGC 3 cut(s) 27, 874, 1600
BccI CCATC 2 cut(s) 65, 1004
BceAI ACGGC 2 cut(s) 28, 112
BcgI CGANNNNNNTGC 4 cut(s) 128, 162, 930, 964
BcoDI GTCTC 2 cut(s) 426, 1456
BfaI CTAG 2 cut(s) 258, 986
BfoI RGCGCY 2 cut(s) 41, 66
BfrI CTTAAG 1 cut(s) 1291
BfuAI ACCTGC 1 cut(s) 1307
BisI GCNGC 3 cut(s) 41, 888, 1614
BlpI GCTNAGC 1 cut(s) 50
BlsI GCNGC 3 cut(s) 42, 889, 1615
Bme18I GGWCC 2 cut(s) 75, 322
BmeT110I CYCGRG 1 cut(s) 302
BmgT120I GGNCC 3 cut(s) 75, 322, 1000
BmiI GGNNCC 3 cut(s) 76, 318, 1629
BmsI GCATC 5 cut(s) 820, 865, 972, 1600, 1625
BpiI GAAGAC 1 cut(s) 1265
BplI GAGNNNNNCTC 2 cut(s) 182, 214
BpmI CTGGAG 1 cut(s) 986
Bpu10I CCTNAGC 1 cut(s) 512
Bpu1102I GCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 203, 223
BsaBI GATNNNNATC 1 cut(s) 544
BsaJI CCNNGG 1 cut(s) 527
BsaWI WCCGGW 1 cut(s) 319
BsaXI ACNNNNNCTCC 2 cut(s) 953, 983
Bsc4I CCNNNNNNNGG 2 cut(s) 72, 709
Bse1I ACTGG 1 cut(s) 721
Bse3DI GCAATG 1 cut(s) 1143
Bse8I GATNNNNATC 1 cut(s) 544
BseDI CCNNGG 1 cut(s) 527
BseGI GGATG 8 cut(s) 130, 193, 445, 545, 856, 987, 1345, 1615
BseJI GATNNNNATC 1 cut(s) 544
BseLI CCNNNNNNNGG 2 cut(s) 72, 709
BseMI GCAATG 1 cut(s) 1143
BseMII CTCAG 2 cut(s) 857, 1403
BseNI ACTGG 1 cut(s) 721
BseRI GAGGAG 2 cut(s) 974, 1449
BseXI GCAGC 3 cut(s) 27, 874, 1600
BsgI GTGCAG 1 cut(s) 830
BshFI GGCC 3 cut(s) 99, 1002, 1134
BshNI GGYRCC 1 cut(s) 316
BsiHKCI CYCGRG 1 cut(s) 302
BsiSI CCGG 2 cut(s) 66, 320
BslFI GGGAC 1 cut(s) 88
BslI CCNNNNNNNGG 2 cut(s) 72, 709
BsmAI GTCTC 2 cut(s) 426, 1456
BsmFI GGGAC 1 cut(s) 88
BsnI GGCC 3 cut(s) 99, 1002, 1134
BsoBI CYCGRG 1 cut(s) 302
Bsp143I GATC 2 cut(s) 135, 292
Bsp1720I GCTNAGC 1 cut(s) 50
BspACI CCGC 5 cut(s) 22, 78, 132, 217, 659
BspANI GGCC 3 cut(s) 99, 1002, 1134
BspCNI CTCAG 2 cut(s) 856, 1404
BspLI GGNNCC 3 cut(s) 76, 318, 1629
BspMI ACCTGC 1 cut(s) 1307
BspPI GGATC 1 cut(s) 143
BspT107I GGYRCC 1 cut(s) 316
BspTI CTTAAG 1 cut(s) 1291
BsrDI GCAATG 1 cut(s) 1143
BsrI ACTGG 1 cut(s) 721
BssECI CCNNGG 1 cut(s) 527
BssMI GATC 2 cut(s) 135, 292
Bst4CI ACNGT 3 cut(s) 369, 1279, 1424
Bst6I CTCTTC 1 cut(s) 1459
BstAFI CTTAAG 1 cut(s) 1291
BstC8I GCNNGC 3 cut(s) 101, 169, 615
BstDEI CTNAG 4 cut(s) 50, 512, 843, 1412
BstF5I GGATG 8 cut(s) 130, 193, 445, 545, 856, 987, 1345, 1615
BstH2I RGCGCY 2 cut(s) 41, 66
BstHHI GCGC 3 cut(s) 40, 65, 1483
BstKTI GATC 2 cut(s) 138, 295
BstMAI GTCTC 2 cut(s) 426, 1456
BstMBI GATC 2 cut(s) 135, 292
BstMWI GCNNNNNNNGC 3 cut(s) 46, 60, 86
BstNSI RCATGY 3 cut(s) 1026, 1594, 1649
BstV1I GCAGC 3 cut(s) 27, 874, 1600
BstV2I GAAGAC 1 cut(s) 1265
BstXI CCANNNNNNTGG 2 cut(s) 743, 1010
BsuRI GGCC 3 cut(s) 99, 1002, 1134
BtsCI GGATG 8 cut(s) 130, 193, 445, 545, 856, 987, 1345, 1615
BtsI GCAGTG 2 cut(s) 459, 714
BtsIMutI CAGTG 5 cut(s) 459, 714, 941, 1111, 1255
BveI ACCTGC 1 cut(s) 1307
Cac8I GCNNGC 3 cut(s) 101, 169, 615
CfoI GCGC 3 cut(s) 40, 65, 1483
Cfr13I GGNCC 3 cut(s) 75, 322, 1000
Csp6I GTAC 4 cut(s) 317, 1296, 1420, 1493
CviJI RGCY 9 cut(s) 12, 99, 729, 793, 887, 964, 1002, 1134, 1442
CviKI_1 RGCY 9 cut(s) 12, 99, 729, 793, 887, 964, 1002, 1134, 1442
CviQI GTAC 4 cut(s) 317, 1296, 1420, 1493
DdeI CTNAG 4 cut(s) 50, 512, 843, 1412
DpnI GATC 2 cut(s) 137, 294
DpnII GATC 2 cut(s) 135, 292
EaeI YGGCCR 1 cut(s) 1132
Eam1104I CTCTTC 1 cut(s) 1459
EarI CTCTTC 1 cut(s) 1459
Eco47I GGWCC 2 cut(s) 75, 322
Eco57I CTGAAG 1 cut(s) 1087
Eco88I CYCGRG 1 cut(s) 302
FalI AAGNNNNNCTT 4 cut(s) 96, 128, 165, 197
FaqI GGGAC 1 cut(s) 88
FauI CCCGC 1 cut(s) 125
Fnu4HI GCNGC 3 cut(s) 41, 888, 1614
FokI GGATG 8 cut(s) 137, 200, 452, 552, 843, 994, 1352, 1622
Fsp4HI GCNGC 3 cut(s) 41, 888, 1614
FspBI CTAG 2 cut(s) 258, 986
GlaI GCGC 3 cut(s) 39, 64, 1482
GluI GCNGC 3 cut(s) 41, 888, 1614
GsuI CTGGAG 1 cut(s) 986
HaeII RGCGCY 2 cut(s) 41, 66
HaeIII GGCC 3 cut(s) 99, 1002, 1134
HapII CCGG 2 cut(s) 66, 320
HhaI GCGC 3 cut(s) 40, 65, 1483
Hin6I GCGC 3 cut(s) 38, 63, 1481
HinP1I GCGC 3 cut(s) 38, 63, 1481
HincII GTYRAC 3 cut(s) 19, 954, 1275
HindII GTYRAC 3 cut(s) 19, 954, 1275
HpaI GTTAAC 2 cut(s) 954, 1275
HpaII CCGG 2 cut(s) 66, 320
Hpy166II GTNNAC 4 cut(s) 19, 691, 954, 1275
Hpy188I TCNGA 9 cut(s) 337, 375, 606, 1171, 1317, 1351, 1413, 1537, 1621
Hpy188III TCNNGA 6 cut(s) 139, 182, 258, 281, 452, 1607
Hpy8I GTNNAC 4 cut(s) 19, 691, 954, 1275
HpyAV CCTTC 5 cut(s) 230, 552, 742, 971, 1072
HpyCH4III ACNGT 3 cut(s) 369, 1279, 1424
HpyCH4IV ACGT 3 cut(s) 117, 1045, 1262
HpyF10VI GCNNNNNNNGC 3 cut(s) 46, 60, 86
HpyF3I CTNAG 4 cut(s) 50, 512, 843, 1412
HpySE526I ACGT 3 cut(s) 117, 1045, 1262
HspAI GCGC 3 cut(s) 38, 63, 1481
KpnI GGTACC 1 cut(s) 320
KspAI GTTAAC 2 cut(s) 954, 1275
Kzo9I GATC 2 cut(s) 135, 292
LmnI GCTCC 4 cut(s) 9, 734, 838, 961
Lsp1109I GCAGC 3 cut(s) 27, 874, 1600
LweI GCATC 5 cut(s) 820, 865, 972, 1600, 1625
MaeI CTAG 2 cut(s) 258, 986
MaeII ACGT 3 cut(s) 117, 1045, 1262
MaeIII GTNAC 6 cut(s) 698, 871, 1342, 1474, 1568, 1641
MalI GATC 2 cut(s) 137, 294
MboI GATC 2 cut(s) 135, 292
MboII GAAGA 7 cut(s) 170, 354, 732, 1076, 1093, 1270, 1446
MlsI TGGCCA 1 cut(s) 1134
MluNI TGGCCA 1 cut(s) 1134
MlyI GAGTC 3 cut(s) 214, 601, 1379
MmeI TCCRAC 3 cut(s) 292, 535, 555
MnlI CCTC 8 cut(s) 178, 208, 282, 537, 952, 1357, 1470, 1632
Mox20I TGGCCA 1 cut(s) 1134
MscI TGGCCA 1 cut(s) 1134
MslI CAYNNNNRTG 2 cut(s) 598, 1008
Msp20I TGGCCA 1 cut(s) 1134
MspCI CTTAAG 1 cut(s) 1291
MspI CCGG 2 cut(s) 66, 320
MwoI GCNNNNNNNGC 3 cut(s) 46, 60, 86
NdeII GATC 2 cut(s) 135, 292
NlaIV GGNNCC 3 cut(s) 76, 318, 1629
NmuCI GTSAC 1 cut(s) 1641
NspI RCATGY 3 cut(s) 1026, 1594, 1649
PciI ACATGT 1 cut(s) 1645
PfeI GAWTC 7 cut(s) 332, 376, 448, 455, 484, 590, 1408
PflMI CCANNNNNTGG 1 cut(s) 709
PkrI GCNGC 3 cut(s) 42, 889, 1615
PleI GAGTC 3 cut(s) 213, 601, 1378
PpsI GAGTC 3 cut(s) 213, 601, 1378
PscI ACATGT 1 cut(s) 1645
PsiI TTATAA 1 cut(s) 350
Psp1406I AACGTT 1 cut(s) 117
PspN4I GGNNCC 3 cut(s) 76, 318, 1629
PspPI GGNCC 3 cut(s) 75, 322, 1000
PsrI GAACNNNNNNTAC 2 cut(s) 1580, 1612
RsaI GTAC 4 cut(s) 318, 1297, 1421, 1494
RsaNI GTAC 4 cut(s) 317, 1296, 1420, 1493
RseI CAYNNNNRTG 2 cut(s) 598, 1008
SatI GCNGC 3 cut(s) 41, 888, 1614
Sau3AI GATC 2 cut(s) 135, 292
Sau96I GGNCC 3 cut(s) 75, 322, 1000
SchI GAGTC 3 cut(s) 214, 601, 1379
SfaNI GCATC 5 cut(s) 820, 865, 972, 1600, 1625
SinI GGWCC 2 cut(s) 75, 322
SmiMI CAYNNNNRTG 2 cut(s) 598, 1008
SmlI CTYRAG 3 cut(s) 182, 202, 1291
SmoI CTYRAG 3 cut(s) 182, 202, 1291
SsiI CCGC 5 cut(s) 22, 78, 132, 217, 659
SspMI CTAG 2 cut(s) 258, 986
TaaI ACNGT 3 cut(s) 369, 1279, 1424
TaiI ACGT 3 cut(s) 120, 1048, 1265
TaqI TCGA 2 cut(s) 138, 419
TatI WGTACW 1 cut(s) 1419
TfiI GAWTC 7 cut(s) 332, 376, 448, 455, 484, 590, 1408
TscAI CASTG 5 cut(s) 466, 714, 948, 1111, 1255
TseFI GTSAC 1 cut(s) 1641
TseI GCWGC 3 cut(s) 40, 887, 1613
Tsp45I GTSAC 1 cut(s) 1641
TspDTI ATGAA 9 cut(s) 16, 234, 497, 582, 731, 939, 1178, 1398, 1572
TspRI CASTG 5 cut(s) 466, 714, 948, 1111, 1255
Van91I CCANNNNNTGG 1 cut(s) 709
Vha464I CTTAAG 1 cut(s) 1291
VpaK11BI GGWCC 2 cut(s) 75, 322
XapI RAATTY 5 cut(s) 563, 621, 1067, 1199, 1286
XbaI TCTAGA 1 cut(s) 257
XceI RCATGY 3 cut(s) 1026, 1594, 1649
XspI CTAG 2 cut(s) 258, 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.