Rw6G009390

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0017822
Physical Location & Seq
Forward (+)
15695821 .. 15697334
1514 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G009390.1

Sequence Viewer

Length: 1350 bp
ATGCTGGAGAAAGCCCTGGGCCATGACGATGGGGTTCTTGCATGTTCTAAAACATATTCAAAACATGAATTGTTTGCATTGATTAAATTTGATTTGAAGGTTCTGCTGTCATGTGTTTTCTTAAGTGGGGGTAATAATTATAGTTTGATTGGCCAACCAGACATTGCAACCCTATATCTTATGAGGTCAGAAAAGTTGGTAGAGTTTATTATTGAGAAATTTAATCCTTTTGACTTGCCTATTATGGAATATGTGGATTTGCAAGTCAATGTTTTGAGGATGTTTGACAGGCTAACAGTAGTTAAGAAAAGTACCTGCTCTTTGTCATATCAGAGTAGTGGCAAATCATCAATGGATGGTACCTCTGATAAATTATTTAATAGTGAGTCTTTGACCTATGAACATTCGGTTGTGGTTGTTGAGAATCTGAGAAAGTACAGTCTGTTTTTTGTGAAAGCTCTCCATGCTCTTCTCCTCTTGCAGTTATTTAAAGTAAATGTGCTGTATTTGGTACAAAGATTTTGTGAGAATGTTATTGTTTTTAATGAGAAATCAGACACAGAAACTTGTTTCTATGAAGTGTATGGTAATGATAGAATTATTGGGAGCATGCTTTACTCAATTCTGGATGCTGCATCTGATAGGGAACCAAAAGTGAGGTCACATGTTGCAATAGTATTGGAGCTGTTACTACAGGCAAGGCTTGTACATCCTCTTTACTTTTATTGCATGGCTGAAGTGGTGTTGGGTAAACTCGGTGATCCTGATAATGATATAAAAAATGCATTTGTTAGACTGCTTGCTATTGTTGTACCTACTACATTGTATGCATGTGGGCTTCATGATTATGGGACATCTTCTTCATCTAGGGCCGGTGCTGTTCAGGTAGGCAACAGCTGCAGAAGTTCAAAAGACCTTGTTCCACATCAACTGGAGGAAACAGGAAATGTCGCCACTGGTGTGTGGTTGGACATCAAAGTGGATGATGGTTTTCTTGAAAAACATTGCTCAGTAAATAATCTGGCTGGTGCTTGGTGGGCTGTCCAAGAAGCTGCTAGATATTGTATTTCCACATGGCTTCGGACTAACCTTGGTGGGCCAACCCAGACTTCTGCAGCAATAGAGCGAATGCTTTTGGATGTTGCGCACCTATTACAGTATGATAGTGAACAGACCGTTGGGAACTCGAGTATGATACGGTCTTCTGGTGCTCATTTGCTACCAGTGGTCCTTGCAATAATTGAAGGTCTACATGATGTTCAGAGTCTAGGAAAGGAGGCTGCTGCAGTTCATCGTTCTCTTGTGGAAGACCTTTCAAAGCTTTCCCTCGTCCCATATTTAATGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000184 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004672 GO:0004674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006401 GO:0006402 GO:0006464 GO:0006468 GO:0006629 GO:0006644 GO:0006650 GO:0006725 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006974 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009892 GO:0009987 GO:0010468 GO:0010605 GO:0010629 GO:0016070 GO:0016071 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0018105 GO:0018193 GO:0018209 GO:0019219 GO:0019222 GO:0019439 GO:0019538 GO:0019637 GO:0030258 GO:0031323 GO:0031974 GO:0031981 GO:0032204 GO:0033043 GO:0033044 GO:0033554 GO:0034641 GO:0034655 GO:0036211 GO:0042162 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043412 GO:0043565 GO:0044237 GO:0044238 GO:0044248 GO:0044255 GO:0044260 GO:0044265 GO:0044267 GO:0044270 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0046486 GO:0046488 GO:0046700 GO:0046777 GO:0046834 GO:0046854 GO:0048519 GO:0048583 GO:0050789 GO:0050794 GO:0050896 GO:0051052 GO:0051128 GO:0051171 GO:0051716 GO:0060255 GO:0065007 GO:0065008 GO:0070013 GO:0071704 GO:0080090 GO:0080134 GO:0080135 GO:0090304 GO:0097159 GO:0140096 GO:1901360 GO:1901361 GO:1901363 GO:1901564 GO:1901575 GO:2001020
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
FATC PI3_PI4_kinase SMG1 WD40

Protein Analysis

449

Amino Acids

49.97

Weight (kDa)

5.73

Isoelectric Point (pI)

33.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SMG1 PF15785 341 - 387 1.4e-06 Serine/threonine-protein kinase smg-1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 1146
Acc36I ACCTGC 1 cut(s) 323
Acc65I GGTACC 1 cut(s) 359
AccB1I GGYRCC 1 cut(s) 359
AccI GTMKAC 1 cut(s) 1249
AclWI GGATC 1 cut(s) 755
AcoI YGGCCR 1 cut(s) 151
AcsI RAATTY 2 cut(s) 86, 218
AcuI CTGAAG 1 cut(s) 756
AfaI GTAC 6 cut(s) 313, 361, 437, 513, 708, 813
AflII CTTAAG 1 cut(s) 121
AflIII ACRYGT 1 cut(s) 664
AgsI TTSAA 6 cut(s) 60, 97, 909, 998, 1244, 1317
AjnI CCWGG 1 cut(s) 15
AjuI GAANNNNNNNTTGG 4 cut(s) 1093, 1125, 1161, 1193
AleI CACNNNNGTG 1 cut(s) 959
AluBI AGCT 5 cut(s) 458, 685, 897, 1052, 1321
AluI AGCT 5 cut(s) 458, 685, 897, 1052, 1321
Alw21I GWGCWC 1 cut(s) 1213
AlwI GGATC 1 cut(s) 755
Ama87I CYCGRG 1 cut(s) 1186
AoxI GGCC 4 cut(s) 19, 151, 870, 1097
ApeKI GCWGC 6 cut(s) 632, 897, 1052, 1115, 1280, 1283
ApoI RAATTY 2 cut(s) 86, 218
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
Asp718I GGTACC 1 cut(s) 359
AspLEI GCGC 1 cut(s) 1147
AspS9I GGNCC 4 cut(s) 19, 870, 1097, 1228
AsuHPI GGTGA 1 cut(s) 770
AvaI CYCGRG 1 cut(s) 1186
AvaII GGWCC 1 cut(s) 1228
BalI TGGCCA 1 cut(s) 153
BanI GGYRCC 1 cut(s) 359
BbsI GAAGAC 2 cut(s) 1194, 1314
Bbv12I GWGCWC 1 cut(s) 1213
BbvI GCAGC 6 cut(s) 619, 884, 1039, 1127, 1267, 1270
BccI CCATC 3 cut(s) 23, 350, 980
BciT130I CCWGG 1 cut(s) 17
BfaI CTAG 3 cut(s) 867, 1056, 1268
BfmI CTRYAG 4 cut(s) 692, 898, 1113, 1284
BfrI CTTAAG 1 cut(s) 121
BfuAI ACCTGC 1 cut(s) 323
BisI GCNGC 6 cut(s) 633, 898, 1053, 1116, 1281, 1284
BlsI GCNGC 6 cut(s) 634, 899, 1054, 1117, 1282, 1285
Bme1390I CCNGG 1 cut(s) 17
Bme18I GGWCC 1 cut(s) 1228
BmeT110I CYCGRG 1 cut(s) 1186
BmgT120I GGNCC 4 cut(s) 19, 870, 1097, 1228
BmiI GGNNCC 2 cut(s) 361, 648
BmrFI CCNGG 1 cut(s) 17
BmsI GCATC 2 cut(s) 619, 644
BpiI GAAGAC 2 cut(s) 1194, 1314
BpmI CTGGAG 2 cut(s) 26, 953
BsaJI CCNNGG 3 cut(s) 15, 16, 1090
Bse118I RCCGGY 1 cut(s) 872
Bse1I ACTGG 3 cut(s) 936, 961, 1223
Bse3DI GCAATG 2 cut(s) 162, 1003
BseBI CCWGG 1 cut(s) 17
BseDI CCNNGG 3 cut(s) 15, 16, 1090
BseGI GGATG 6 cut(s) 285, 361, 634, 709, 988, 1144
BseMI GCAATG 2 cut(s) 162, 1003
BseMII CTCAG 2 cut(s) 419, 1023
BseNI ACTGG 3 cut(s) 936, 961, 1223
BseRI GAGGAG 1 cut(s) 464
BseXI GCAGC 6 cut(s) 619, 884, 1039, 1127, 1267, 1270
BshFI GGCC 4 cut(s) 21, 153, 872, 1099
BshNI GGYRCC 1 cut(s) 359
BsiHKAI GWGCWC 1 cut(s) 1213
BsiHKCI CYCGRG 1 cut(s) 1186
BsiSI CCGG 1 cut(s) 873
BslFI GGGAC 2 cut(s) 865, 1316
BsmFI GGGAC 2 cut(s) 865, 1316
BsmI GAATGC 1 cut(s) 1134
BsnI GGCC 4 cut(s) 21, 153, 872, 1099
BsoBI CYCGRG 1 cut(s) 1186
Bsp1286I GDGCHC 1 cut(s) 1213
Bsp1407I TGTACA 1 cut(s) 706
Bsp143I GATC 1 cut(s) 760
BspANI GGCC 4 cut(s) 21, 153, 872, 1099
BspCNI CTCAG 2 cut(s) 420, 1022
BspHI TCATGA 1 cut(s) 841
BspLI GGNNCC 2 cut(s) 361, 648
BspMAI CTGCAG 3 cut(s) 902, 1117, 1288
BspMI ACCTGC 1 cut(s) 323
BspPI GGATC 1 cut(s) 755
BspQI GCTCTTC 1 cut(s) 474
BspT107I GGYRCC 1 cut(s) 359
BspTI CTTAAG 1 cut(s) 121
BsrDI GCAATG 2 cut(s) 162, 1003
BsrFI RCCGGY 1 cut(s) 872
BsrGI TGTACA 1 cut(s) 706
BsrI ACTGG 3 cut(s) 936, 961, 1223
BssAI RCCGGY 1 cut(s) 872
BssECI CCNNGG 3 cut(s) 15, 16, 1090
BssMI GATC 1 cut(s) 760
BssT1I CCWWGG 1 cut(s) 1090
Bst2UI CCWGG 1 cut(s) 17
Bst4CI ACNGT 5 cut(s) 298, 440, 1158, 1177, 1200
Bst6I CTCTTC 1 cut(s) 474
BstAFI CTTAAG 1 cut(s) 121
BstAPI GCANNNNNTGC 1 cut(s) 897
BstAUI TGTACA 1 cut(s) 706
BstC8I GCNNGC 2 cut(s) 611, 801
BstDEI CTNAG 2 cut(s) 428, 1009
BstF5I GGATG 6 cut(s) 285, 361, 634, 709, 988, 1144
BstHHI GCGC 1 cut(s) 1147
BstKTI GATC 1 cut(s) 763
BstMBI GATC 1 cut(s) 760
BstMWI GCNNNNNNNGC 3 cut(s) 464, 897, 1037
BstNI CCWGG 1 cut(s) 17
BstNSI RCATGY 4 cut(s) 45, 613, 668, 834
BstSCI CCNGG 1 cut(s) 15
BstSFI CTRYAG 4 cut(s) 692, 898, 1113, 1284
BstV1I GCAGC 6 cut(s) 619, 884, 1039, 1127, 1267, 1270
BstV2I GAAGAC 2 cut(s) 1194, 1314
BstXI CCANNNNNNTGG 1 cut(s) 29
BsuRI GGCC 4 cut(s) 21, 153, 872, 1099
BtsCI GGATG 6 cut(s) 285, 361, 634, 709, 988, 1144
BtsIMutI CAGTG 2 cut(s) 954, 1230
BveI ACCTGC 1 cut(s) 323
Cac8I GCNNGC 2 cut(s) 611, 801
CciI TCATGA 1 cut(s) 841
CfoI GCGC 1 cut(s) 1147
Cfr10I RCCGGY 1 cut(s) 872
Cfr13I GGNCC 4 cut(s) 19, 870, 1097, 1228
Csp6I GTAC 6 cut(s) 312, 360, 436, 512, 707, 812
CviQI GTAC 6 cut(s) 312, 360, 436, 512, 707, 812
DdeI CTNAG 2 cut(s) 428, 1009
DpnI GATC 1 cut(s) 762
DpnII GATC 1 cut(s) 760
DraI TTTAAA 1 cut(s) 490
EaeI YGGCCR 1 cut(s) 151
Eam1104I CTCTTC 1 cut(s) 474
EarI CTCTTC 1 cut(s) 474
Eco130I CCWWGG 1 cut(s) 1090
Eco47I GGWCC 1 cut(s) 1228
Eco57I CTGAAG 1 cut(s) 756
Eco88I CYCGRG 1 cut(s) 1186
EcoRII CCWGG 1 cut(s) 15
EcoT14I CCWWGG 1 cut(s) 1090
EcoT22I ATGCAT 2 cut(s) 787, 832
ErhI CCWWGG 1 cut(s) 1090
FaqI GGGAC 2 cut(s) 865, 1316
FblI GTMKAC 1 cut(s) 1249
Fnu4HI GCNGC 6 cut(s) 633, 898, 1053, 1116, 1281, 1284
FokI GGATG 6 cut(s) 292, 368, 641, 696, 995, 1151
Fsp4HI GCNGC 6 cut(s) 633, 898, 1053, 1116, 1281, 1284
FspBI CTAG 3 cut(s) 867, 1056, 1268
FspI TGCGCA 1 cut(s) 1146
GlaI GCGC 1 cut(s) 1146
GluI GCNGC 6 cut(s) 633, 898, 1053, 1116, 1281, 1284
GsuI CTGGAG 2 cut(s) 26, 953
HaeIII GGCC 4 cut(s) 21, 153, 872, 1099
HapII CCGG 1 cut(s) 873
HhaI GCGC 1 cut(s) 1147
Hin6I GCGC 1 cut(s) 1145
HinP1I GCGC 1 cut(s) 1145
HindIII AAGCTT 1 cut(s) 1319
HinfI GANTC 3 cut(s) 386, 424, 1264
HpaII CCGG 1 cut(s) 873
HphI GGTGA 1 cut(s) 770
Hpy166II GTNNAC 3 cut(s) 752, 1169, 1250
Hpy188I TCNGA 8 cut(s) 190, 333, 367, 429, 556, 640, 1083, 1263
Hpy188III TCNNGA 4 cut(s) 626, 764, 842, 995
Hpy8I GTNNAC 3 cut(s) 752, 1169, 1250
HpyAV CCTTC 2 cut(s) 91, 1238
HpyCH4III ACNGT 5 cut(s) 298, 440, 1158, 1177, 1200
HpyF10VI GCNNNNNNNGC 3 cut(s) 464, 897, 1037
HpyF3I CTNAG 2 cut(s) 428, 1009
HspAI GCGC 1 cut(s) 1145
KpnI GGTACC 1 cut(s) 363
Kzo9I GATC 1 cut(s) 760
LguI GCTCTTC 1 cut(s) 474
LmnI GCTCC 2 cut(s) 606, 682
Lsp1109I GCAGC 6 cut(s) 619, 884, 1039, 1127, 1267, 1270
LweI GCATC 2 cut(s) 619, 644
MaeI CTAG 3 cut(s) 867, 1056, 1268
MaeIII GTNAC 2 cut(s) 660, 687
MalI GATC 1 cut(s) 762
MboI GATC 1 cut(s) 760
MboII GAAGA 5 cut(s) 461, 849, 852, 1194, 1319
MhlI GDGCHC 1 cut(s) 1213
MlsI TGGCCA 1 cut(s) 153
MluCI AATT 8 cut(s) 68, 86, 136, 218, 371, 597, 621, 1239
MluNI TGGCCA 1 cut(s) 153
MlyI GAGTC 2 cut(s) 395, 1273
MmeI TCCRAC 1 cut(s) 948
MnlI CCTC 9 cut(s) 177, 270, 373, 485, 651, 723, 928, 1270, 1337
Mox20I TGGCCA 1 cut(s) 153
Mph1103I ATGCAT 2 cut(s) 787, 832
MscI TGGCCA 1 cut(s) 153
MseI TTAA 9 cut(s) 84, 122, 222, 303, 378, 489, 543, 1340, 1348
MslI CAYNNNNRTG 4 cut(s) 27, 846, 959, 977
Msp20I TGGCCA 1 cut(s) 153
MspA1I CMGCKG 1 cut(s) 897
MspCI CTTAAG 1 cut(s) 121
MspI CCGG 1 cut(s) 873
MspR9I CCNGG 1 cut(s) 17
Mva1269I GAATGC 1 cut(s) 1134
MvaI CCWGG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 464, 897, 1037
NdeII GATC 1 cut(s) 760
NlaIV GGNNCC 2 cut(s) 361, 648
NmuCI GTSAC 1 cut(s) 660
NsbI TGCGCA 1 cut(s) 1146
NsiI ATGCAT 2 cut(s) 787, 832
NspI RCATGY 4 cut(s) 45, 613, 668, 834
OliI CACNNNNGTG 1 cut(s) 959
PaeI GCATGC 1 cut(s) 613
PaeR7I CTCGAG 1 cut(s) 1186
PagI TCATGA 1 cut(s) 841
PasI CCCWGGG 1 cut(s) 16
PciI ACATGT 1 cut(s) 664
PciSI GCTCTTC 1 cut(s) 474
PctI GAATGC 1 cut(s) 1134
PfeI GAWTC 1 cut(s) 424
PkrI GCNGC 6 cut(s) 634, 899, 1054, 1117, 1282, 1285
PleI GAGTC 2 cut(s) 394, 1272
PpsI GAGTC 2 cut(s) 394, 1272
PscI ACATGT 1 cut(s) 664
Psp6I CCWGG 1 cut(s) 15
PspGI CCWGG 1 cut(s) 15
PspN4I GGNNCC 2 cut(s) 361, 648
PspPI GGNCC 4 cut(s) 19, 870, 1097, 1228
PspXI VCTCGAGB 1 cut(s) 1186
PstI CTGCAG 3 cut(s) 902, 1117, 1288
PvuII CAGCTG 1 cut(s) 897
RsaI GTAC 6 cut(s) 313, 361, 437, 513, 708, 813
RsaNI GTAC 6 cut(s) 312, 360, 436, 512, 707, 812
RseI CAYNNNNRTG 4 cut(s) 27, 846, 959, 977
SapI GCTCTTC 1 cut(s) 474
SaqAI TTAA 9 cut(s) 84, 122, 222, 303, 378, 489, 543, 1340, 1348
SatI GCNGC 6 cut(s) 633, 898, 1053, 1116, 1281, 1284
Sau3AI GATC 1 cut(s) 760
Sau96I GGNCC 4 cut(s) 19, 870, 1097, 1228
SchI GAGTC 2 cut(s) 395, 1273
ScrFI CCNGG 1 cut(s) 17
SduI GDGCHC 1 cut(s) 1213
SfaNI GCATC 2 cut(s) 619, 644
SfcI CTRYAG 4 cut(s) 692, 898, 1113, 1284
Sfr274I CTCGAG 1 cut(s) 1186
SinI GGWCC 1 cut(s) 1228
SlaI CTCGAG 1 cut(s) 1186
SmiMI CAYNNNNRTG 4 cut(s) 27, 846, 959, 977
SmlI CTYRAG 2 cut(s) 121, 1186
SmoI CTYRAG 2 cut(s) 121, 1186
SphI GCATGC 1 cut(s) 613
Sse9I AATT 8 cut(s) 68, 86, 136, 218, 371, 597, 621, 1239
SspMI CTAG 3 cut(s) 867, 1056, 1268
StyD4I CCNGG 1 cut(s) 15
StyI CCWWGG 1 cut(s) 1090
TaaI ACNGT 5 cut(s) 298, 440, 1158, 1177, 1200
TaqI TCGA 1 cut(s) 1187
TasI AATT 8 cut(s) 68, 86, 136, 218, 371, 597, 621, 1239
TatI WGTACW 2 cut(s) 435, 706
TfiI GAWTC 1 cut(s) 424
Tru1I TTAA 9 cut(s) 84, 122, 222, 303, 378, 489, 543, 1340, 1348
Tru9I TTAA 9 cut(s) 84, 122, 222, 303, 378, 489, 543, 1340, 1348
TscAI CASTG 2 cut(s) 961, 1230
TseFI GTSAC 1 cut(s) 660
TseI GCWGC 6 cut(s) 632, 897, 1052, 1115, 1280, 1283
Tsp45I GTSAC 1 cut(s) 660
TspDTI ATGAA 6 cut(s) 81, 414, 591, 830, 852, 1280
TspRI CASTG 2 cut(s) 961, 1230
Vha464I CTTAAG 1 cut(s) 121
VpaK11BI GGWCC 1 cut(s) 1228
XapI RAATTY 2 cut(s) 86, 218
XceI RCATGY 4 cut(s) 45, 613, 668, 834
XhoI CTCGAG 1 cut(s) 1186
XmiI GTMKAC 1 cut(s) 1249
XspI CTAG 3 cut(s) 867, 1056, 1268
Zsp2I ATGCAT 2 cut(s) 787, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.