Rmu_sc0004924.1_g000038

Zinc knuckle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0004924.1
OG0019377
Physical Location & Seq
Forward (+)
165422 .. 165947
526 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0004924.1_g000038.1.cds

Sequence Viewer

Length: 348 bp
atggcagaagcgatcgatcgtctagctactgccatgacacaatatgattcgacggtggtctcgctgggggctgcggctgttcaagaggggcattttctgtttgagagtgttgttgtgcgtaacaggattatgcatggggggccgtggttttatcgtaacaccatgcttgtgttgggtgagtatgatggtctgggtcctgtggaatctgtgccgcttcatctgatggaagcctgggttgctgtgaaaggattgccgattgcgctgaagaataaagtggcattcgtaaggaagaggagcaccgcgtcaagatcgttttcgatgtacgtaggcgaattcgcacctggatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 zf-CCHC_4

Protein Analysis

115

Amino Acids

12.62

Weight (kDa)

8.09

Isoelectric Point (pI)

43.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 302
AciI CCGC 3 cut(s) 74, 212, 300
AcsI RAATTY 1 cut(s) 332
AcuI CTGAAG 1 cut(s) 284
AfaI GTAC 1 cut(s) 323
AgsI TTSAA 1 cut(s) 83
AjnI CCWGG 2 cut(s) 230, 340
AluBI AGCT 1 cut(s) 26
AluI AGCT 1 cut(s) 26
Alw21I GWGCWC 1 cut(s) 299
Alw26I GTCTC 1 cut(s) 64
AoxI GGCC 1 cut(s) 140
ApeKI GCWGC 1 cut(s) 71
ApoI RAATTY 1 cut(s) 332
AspLEI GCGC 1 cut(s) 262
AspS9I GGNCC 2 cut(s) 140, 194
AsuHPI GGTGA 1 cut(s) 188
AvaII GGWCC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 299
BbvI GCAGC 1 cut(s) 58
BccI CCATC 2 cut(s) 179, 217
BceAI ACGGC 1 cut(s) 127
BciT130I CCWGG 2 cut(s) 232, 342
BcoDI GTCTC 1 cut(s) 64
BfaI CTAG 1 cut(s) 23
BisI GCNGC 3 cut(s) 72, 75, 212
BlsI GCNGC 3 cut(s) 73, 76, 213
Bme1390I CCNGG 2 cut(s) 232, 342
Bme18I GGWCC 1 cut(s) 194
BmgT120I GGNCC 2 cut(s) 140, 194
BmiI GGNNCC 2 cut(s) 141, 195
BmrFI CCNGG 2 cut(s) 232, 342
BoxI GACNNNNGTC 1 cut(s) 56
Bsa29I ATCGAT 1 cut(s) 15
BsaAI YACGTR 1 cut(s) 325
BsaI GGTCTC 1 cut(s) 64
BsaJI CCNNGG 2 cut(s) 143, 231
BseBI CCWGG 2 cut(s) 232, 342
BseCI ATCGAT 1 cut(s) 15
BseDI CCNNGG 2 cut(s) 143, 231
BseRI GAGGAG 1 cut(s) 307
BseXI GCAGC 1 cut(s) 58
BseYI CCCAGC 1 cut(s) 64
Bsh1236I CGCG 1 cut(s) 302
Bsh1285I CGRYCG 2 cut(s) 15, 19
BshFI GGCC 1 cut(s) 142
BshVI ATCGAT 1 cut(s) 15
BsiEI CGRYCG 2 cut(s) 15, 19
BsiHKAI GWGCWC 1 cut(s) 299
BsmAI GTCTC 1 cut(s) 64
BsmI GAATGC 1 cut(s) 278
BsnI GGCC 1 cut(s) 142
Bso31I GGTCTC 1 cut(s) 64
Bsp1286I GDGCHC 1 cut(s) 299
Bsp143I GATC 3 cut(s) 12, 16, 308
BspACI CCGC 3 cut(s) 74, 212, 300
BspANI GGCC 1 cut(s) 142
BspDI ATCGAT 1 cut(s) 15
BspFNI CGCG 1 cut(s) 302
BspLI GGNNCC 2 cut(s) 141, 195
BspTNI GGTCTC 1 cut(s) 64
BssECI CCNNGG 2 cut(s) 143, 231
BssMI GATC 3 cut(s) 12, 16, 308
Bst2UI CCWGG 2 cut(s) 232, 342
Bst4CI ACNGT 1 cut(s) 55
Bst6I CTCTTC 1 cut(s) 284
BstBAI YACGTR 1 cut(s) 325
BstDSI CCRYGG 1 cut(s) 143
BstFNI CGCG 1 cut(s) 302
BstHHI GCGC 1 cut(s) 262
BstKTI GATC 3 cut(s) 15, 19, 311
BstMAI GTCTC 1 cut(s) 64
BstMBI GATC 3 cut(s) 12, 16, 308
BstMCI CGRYCG 2 cut(s) 15, 19
BstMWI GCNNNNNNNGC 3 cut(s) 139, 236, 259
BstNI CCWGG 2 cut(s) 232, 342
BstPAI GACNNNNGTC 1 cut(s) 56
BstSCI CCNGG 2 cut(s) 230, 340
BstSNI TACGTA 1 cut(s) 325
BstUI CGCG 1 cut(s) 302
BstV1I GCAGC 1 cut(s) 58
Bsu15I ATCGAT 1 cut(s) 15
BsuRI GGCC 1 cut(s) 142
BsuTUI ATCGAT 1 cut(s) 15
BtgI CCRYGG 1 cut(s) 143
CfoI GCGC 1 cut(s) 262
Cfr13I GGNCC 2 cut(s) 140, 194
ClaI ATCGAT 1 cut(s) 15
CseI GACGC 1 cut(s) 291
Csp6I GTAC 1 cut(s) 322
CviAII CATG 3 cut(s) 34, 134, 163
CviJI RGCY 5 cut(s) 26, 71, 77, 142, 230
CviKI_1 RGCY 5 cut(s) 26, 71, 77, 142, 230
CviQI GTAC 1 cut(s) 322
DpnI GATC 3 cut(s) 14, 18, 310
DpnII GATC 3 cut(s) 12, 16, 308
Eam1104I CTCTTC 1 cut(s) 284
EarI CTCTTC 1 cut(s) 284
Eco105I TACGTA 1 cut(s) 325
Eco31I GGTCTC 1 cut(s) 64
Eco47I GGWCC 1 cut(s) 194
Eco57I CTGAAG 1 cut(s) 284
EcoO109I RGGNCCY 1 cut(s) 194
EcoRI GAATTC 1 cut(s) 332
EcoRII CCWGG 2 cut(s) 230, 340
EcoT22I ATGCAT 1 cut(s) 135
FaeI CATG 3 cut(s) 37, 137, 166
FaiI YATR 6 cut(s) 35, 45, 131, 135, 164, 183
FatI CATG 3 cut(s) 33, 133, 162
Fnu4HI GCNGC 3 cut(s) 72, 75, 212
Fsp4HI GCNGC 3 cut(s) 72, 75, 212
FspBI CTAG 1 cut(s) 23
GlaI GCGC 1 cut(s) 261
GluI GCNGC 3 cut(s) 72, 75, 212
GsaI CCCAGC 1 cut(s) 68
HaeIII GGCC 1 cut(s) 142
HgaI GACGC 1 cut(s) 291
HhaI GCGC 1 cut(s) 262
Hin1II CATG 3 cut(s) 37, 137, 166
Hin6I GCGC 1 cut(s) 260
HinP1I GCGC 1 cut(s) 260
HinfI GANTC 2 cut(s) 47, 203
HphI GGTGA 1 cut(s) 188
Hpy188I TCNGA 1 cut(s) 222
Hpy188III TCNNGA 2 cut(s) 83, 306
Hpy99I CGWCG 1 cut(s) 55
HpyCH4III ACNGT 1 cut(s) 55
HpyCH4IV ACGT 1 cut(s) 324
HpyCH4V TGCA 1 cut(s) 133
HpyF10VI GCNNNNNNNGC 3 cut(s) 139, 236, 259
HpySE526I ACGT 1 cut(s) 324
Hsp92II CATG 3 cut(s) 37, 137, 166
HspAI GCGC 1 cut(s) 260
Kzo9I GATC 3 cut(s) 12, 16, 308
LmnI GCTCC 1 cut(s) 294
LpnPI CCDG 7 cut(s) 50, 109, 176, 210, 217, 244, 327
Lsp1109I GCAGC 1 cut(s) 58
MaeI CTAG 1 cut(s) 23
MaeII ACGT 1 cut(s) 324
MaeIII GTNAC 2 cut(s) 119, 155
MalI GATC 3 cut(s) 14, 18, 310
MboI GATC 3 cut(s) 12, 16, 308
MboII GAAGA 2 cut(s) 277, 301
MhlI GDGCHC 1 cut(s) 299
MluCI AATT 1 cut(s) 332
MnlI CCTC 2 cut(s) 79, 285
Mph1103I ATGCAT 1 cut(s) 135
MslI CAYNNNNRTG 2 cut(s) 167, 343
MspR9I CCNGG 2 cut(s) 232, 342
Mva1269I GAATGC 1 cut(s) 278
MvaI CCWGG 2 cut(s) 232, 342
MvnI CGCG 1 cut(s) 302
MwoI GCNNNNNNNGC 3 cut(s) 139, 236, 259
NdeII GATC 3 cut(s) 12, 16, 308
NlaIII CATG 3 cut(s) 37, 137, 166
NlaIV GGNNCC 2 cut(s) 141, 195
NsiI ATGCAT 1 cut(s) 135
PctI GAATGC 1 cut(s) 278
PfeI GAWTC 2 cut(s) 47, 203
PkrI GCNGC 3 cut(s) 73, 76, 213
Ple19I CGATCG 2 cut(s) 15, 19
Ppu21I YACGTR 1 cut(s) 325
PpuMI RGGWCCY 1 cut(s) 194
PshAI GACNNNNGTC 1 cut(s) 56
Psp5II RGGWCCY 1 cut(s) 194
Psp6I CCWGG 2 cut(s) 230, 340
PspFI CCCAGC 1 cut(s) 64
PspGI CCWGG 2 cut(s) 230, 340
PspN4I GGNNCC 2 cut(s) 141, 195
PspPI GGNCC 2 cut(s) 140, 194
PspPPI RGGWCCY 1 cut(s) 194
PvuI CGATCG 2 cut(s) 15, 19
RsaI GTAC 1 cut(s) 323
RsaNI GTAC 1 cut(s) 322
RseI CAYNNNNRTG 2 cut(s) 167, 343
SatI GCNGC 3 cut(s) 72, 75, 212
Sau3AI GATC 3 cut(s) 12, 16, 308
Sau96I GGNCC 2 cut(s) 140, 194
ScrFI CCNGG 2 cut(s) 232, 342
SduI GDGCHC 1 cut(s) 299
SetI ASST 3 cut(s) 28, 327, 343
SinI GGWCC 1 cut(s) 194
SmiMI CAYNNNNRTG 2 cut(s) 167, 343
SnaBI TACGTA 1 cut(s) 325
Sse9I AATT 1 cut(s) 332
SsiI CCGC 3 cut(s) 74, 212, 300
SspMI CTAG 1 cut(s) 23
StyD4I CCNGG 2 cut(s) 230, 340
TaaI ACNGT 1 cut(s) 55
TaiI ACGT 1 cut(s) 327
TaqI TCGA 3 cut(s) 15, 50, 317
TasI AATT 1 cut(s) 332
TauI GCSGC 2 cut(s) 77, 214
TfiI GAWTC 2 cut(s) 47, 203
TseI GCWGC 1 cut(s) 71
TspDTI ATGAA 1 cut(s) 206
VpaK11BI GGWCC 1 cut(s) 194
XapI RAATTY 1 cut(s) 332
XcmI CCANNNNNNNNNTGG 1 cut(s) 169
XspI CTAG 1 cut(s) 23
Zsp2I ATGCAT 1 cut(s) 135
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.