Rmu_sc0005400.1_g000006

EXORDIUM-like 2

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005400.1
OG0019508
Physical Location & Seq
Reverse (-)
60757 .. 62018
1262 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 654 bp
atgttattcaatcgggttctagacttgattcatggacttctattgctgaatgcttcatgggattgcaaagttttaaggtgtatcatgcggctcggtttcggtggtcagaaattctccactctagcggagcagcaaccactggtgctcaagttcgataatagcgcccttctcaagggcaacatcaccgtcaatctattatggtatggccactttaccccaatccagcgctccataattgtagacttcatccagtccctcagttcccagcgtcgtgccccactccttccctcaacttcttcctggtggaaaaccaccaagaaatacaagatcggatcttccaccgtcccctccacgagtccctacgacatcttctgcggcgtcccctacactgccctctacaccgaacccagaagaccagaaaagccgtcgtggacgcggcaagaaaaagcccagaaaccgtcagataatttcagcatcgattctcgcaccatggatgacggaagaccaaaacaattccagacggacgtcggcgacgtcgagatggctcgaacactaaatcatccccaaccccaccggccgacactcatcgtcttctccccagatctgatcatcttctctctaaacctcgatttcgagtttgaatcggagctctaa

Protein Analysis

217

Amino Acids

24.85

Weight (kDa)

8.99

Isoelectric Point (pI)

46.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phi_1 PF04674 49 - 110 2.7e-18 Phosphate-induced protein 1 conserved region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 528, 537
AccI GTMKAC 1 cut(s) 240
AccII CGCG 1 cut(s) 436
AciI CCGC 4 cut(s) 88, 125, 375, 436
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 2 cut(s) 205, 575
AcsI RAATTY 1 cut(s) 110
AcyI GRCGYC 3 cut(s) 378, 525, 534
AfeI AGCGCT 1 cut(s) 227
AfiI CCNNNNNNNGG 1 cut(s) 172
AgsI TTSAA 2 cut(s) 10, 641
AjnI CCWGG 1 cut(s) 299
AluBI AGCT 1 cut(s) 649
AluI AGCT 1 cut(s) 649
Alw21I GWGCWC 2 cut(s) 147, 651
AlwI GGATC 1 cut(s) 340
Aor51HI AGCGCT 1 cut(s) 227
AoxI GGCC 2 cut(s) 205, 575
ApeKI GCWGC 1 cut(s) 130
ApoI RAATTY 1 cut(s) 110
AspLEI GCGC 2 cut(s) 164, 228
AsuHPI GGTGA 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 277
BalI TGGCCA 1 cut(s) 207
BanII GRGCYC 1 cut(s) 651
BauI CACGAG 1 cut(s) 352
BbsI GAAGAC 3 cut(s) 418, 508, 583
Bbv12I GWGCWC 2 cut(s) 147, 651
BbvI GCAGC 1 cut(s) 142
BccI CCATC 1 cut(s) 535
BceAI ACGGC 1 cut(s) 409
BciT130I CCWGG 1 cut(s) 301
BclI TGATCA 1 cut(s) 606
BfaI CTAG 2 cut(s) 20, 122
BfoI RGCGCY 2 cut(s) 165, 229
BglII AGATCT 1 cut(s) 601
BisI GCNGC 4 cut(s) 89, 131, 376, 437
BlsI GCNGC 4 cut(s) 90, 132, 377, 438
Bme1390I CCNGG 1 cut(s) 301
BmrFI CCNGG 1 cut(s) 301
BmsI GCATC 1 cut(s) 483
BoxI GACNNNNGTC 1 cut(s) 524
BpiI GAAGAC 3 cut(s) 418, 508, 583
BpuEI CTTGAG 2 cut(s) 131, 155
Bsa29I ATCGAT 1 cut(s) 477
BsaHI GRCGYC 3 cut(s) 378, 525, 534
BsaJI CCNNGG 1 cut(s) 489
Bsc4I CCNNNNNNNGG 1 cut(s) 172
Bse118I RCCGGY 1 cut(s) 573
Bse1I ACTGG 2 cut(s) 144, 250
BseBI CCWGG 1 cut(s) 301
BseCI ATCGAT 1 cut(s) 477
BseDI CCNNGG 1 cut(s) 489
BseGI GGATG 3 cut(s) 246, 499, 559
BseLI CCNNNNNNNGG 1 cut(s) 172
BseMII CTCAG 1 cut(s) 271
BseNI ACTGG 2 cut(s) 144, 250
BseSI GKGCMC 1 cut(s) 277
BseX3I CGGCCG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 142
BseYI CCCAGC 1 cut(s) 264
Bsh1236I CGCG 1 cut(s) 436
Bsh1285I CGRYCG 1 cut(s) 578
BshFI GGCC 2 cut(s) 207, 577
BshVI ATCGAT 1 cut(s) 477
BsiEI CGRYCG 1 cut(s) 578
BsiHKAI GWGCWC 2 cut(s) 147, 651
BsiSI CCGG 1 cut(s) 574
BslFI GGGAC 4 cut(s) 238, 329, 342, 365
BslI CCNNNNNNNGG 1 cut(s) 172
BsmFI GGGAC 4 cut(s) 238, 329, 342, 365
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 2 cut(s) 207, 577
Bsp1286I GDGCHC 3 cut(s) 147, 277, 651
Bsp143I GATC 4 cut(s) 327, 332, 601, 606
Bsp19I CCATGG 1 cut(s) 489
BspACI CCGC 4 cut(s) 88, 125, 375, 436
BspANI GGCC 2 cut(s) 207, 577
BspCNI CTCAG 1 cut(s) 270
BspDI ATCGAT 1 cut(s) 477
BspFNI CGCG 1 cut(s) 436
BspPI GGATC 1 cut(s) 340
BsrFI RCCGGY 1 cut(s) 573
BsrI ACTGG 2 cut(s) 144, 250
BssAI RCCGGY 1 cut(s) 573
BssECI CCNNGG 1 cut(s) 489
BssMI GATC 4 cut(s) 327, 332, 601, 606
BssNI GRCGYC 3 cut(s) 378, 525, 534
BssSI CACGAG 1 cut(s) 352
BssT1I CCWWGG 1 cut(s) 489
Bst2BI CACGAG 1 cut(s) 352
Bst2UI CCWGG 1 cut(s) 301
Bst4CI ACNGT 3 cut(s) 187, 343, 459
BstACI GRCGYC 3 cut(s) 378, 525, 534
BstDEI CTNAG 1 cut(s) 257
BstDSI CCRYGG 1 cut(s) 489
BstENI CCTNNNNNAGG 1 cut(s) 170
BstF5I GGATG 3 cut(s) 246, 499, 559
BstFNI CGCG 1 cut(s) 436
BstH2I RGCGCY 2 cut(s) 165, 229
BstHHI GCGC 2 cut(s) 164, 228
BstKTI GATC 4 cut(s) 330, 335, 604, 609
BstMBI GATC 4 cut(s) 327, 332, 601, 606
BstMCI CGRYCG 1 cut(s) 578
BstNI CCWGG 1 cut(s) 301
BstPAI GACNNNNGTC 1 cut(s) 524
BstSCI CCNGG 1 cut(s) 299
BstSLI GKGCMC 1 cut(s) 277
BstUI CGCG 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 142
BstV2I GAAGAC 3 cut(s) 418, 508, 583
BstX2I RGATCY 2 cut(s) 332, 601
BstYI RGATCY 2 cut(s) 332, 601
BstZI CGGCCG 1 cut(s) 575
Bsu15I ATCGAT 1 cut(s) 477
BsuRI GGCC 2 cut(s) 207, 577
BsuTUI ATCGAT 1 cut(s) 477
BtgI CCRYGG 1 cut(s) 489
BtsCI GGATG 3 cut(s) 246, 499, 559
BtsI GCAGTG 1 cut(s) 387
BtsIMutI CAGTG 2 cut(s) 137, 387
CfoI GCGC 2 cut(s) 164, 228
Cfr10I RCCGGY 1 cut(s) 573
ClaI ATCGAT 1 cut(s) 477
CseI GACGC 3 cut(s) 257, 367, 442
CviAII CATG 4 cut(s) 32, 57, 85, 490
CviJI RGCY 7 cut(s) 91, 207, 424, 449, 545, 577, 649
CviKI_1 RGCY 7 cut(s) 91, 207, 424, 449, 545, 577, 649
DdeI CTNAG 1 cut(s) 257
DpnI GATC 4 cut(s) 329, 334, 603, 608
DpnII GATC 4 cut(s) 327, 332, 601, 606
EaeI YGGCCR 2 cut(s) 205, 575
EagI CGGCCG 1 cut(s) 575
Ecl136II GAGCTC 1 cut(s) 649
EclXI CGGCCG 1 cut(s) 575
Eco130I CCWWGG 1 cut(s) 489
Eco24I GRGCYC 1 cut(s) 651
Eco47III AGCGCT 1 cut(s) 227
Eco52I CGGCCG 1 cut(s) 575
Eco53kI GAGCTC 1 cut(s) 649
EcoICRI GAGCTC 1 cut(s) 649
EcoNI CCTNNNNNAGG 1 cut(s) 170
EcoRII CCWGG 1 cut(s) 299
EcoT14I CCWWGG 1 cut(s) 489
EcoT38I GRGCYC 1 cut(s) 651
ErhI CCWWGG 1 cut(s) 489
FaeI CATG 4 cut(s) 35, 60, 88, 493
FaiI YATR 7 cut(s) 33, 58, 86, 199, 204, 233, 491
FaqI GGGAC 4 cut(s) 238, 329, 342, 365
FatI CATG 4 cut(s) 31, 56, 84, 489
FbaI TGATCA 1 cut(s) 606
FblI GTMKAC 1 cut(s) 240
Fnu4HI GCNGC 4 cut(s) 89, 131, 376, 437
FokI GGATG 3 cut(s) 233, 506, 546
FriOI GRGCYC 1 cut(s) 651
Fsp4HI GCNGC 4 cut(s) 89, 131, 376, 437
FspBI CTAG 2 cut(s) 20, 122
GlaI GCGC 2 cut(s) 163, 227
GluI GCNGC 4 cut(s) 89, 131, 376, 437
GsaI CCCAGC 1 cut(s) 268
HaeII RGCGCY 2 cut(s) 165, 229
HaeIII GGCC 2 cut(s) 207, 577
HapII CCGG 1 cut(s) 574
HgaI GACGC 3 cut(s) 257, 367, 442
HhaI GCGC 2 cut(s) 164, 228
Hin1I GRCGYC 3 cut(s) 378, 525, 534
Hin1II CATG 4 cut(s) 35, 60, 88, 493
Hin6I GCGC 2 cut(s) 162, 226
HinP1I GCGC 2 cut(s) 162, 226
HinfI GANTC 4 cut(s) 28, 355, 479, 641
HpaII CCGG 1 cut(s) 574
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 2 cut(s) 241, 432
Hpy188I TCNGA 5 cut(s) 108, 332, 463, 606, 646
Hpy188III TCNNGA 3 cut(s) 20, 517, 538
Hpy8I GTNNAC 2 cut(s) 241, 432
Hpy99I CGWCG 5 cut(s) 273, 430, 530, 536, 539
HpyAV CCTTC 2 cut(s) 176, 293
HpyCH4III ACNGT 3 cut(s) 187, 343, 459
HpyCH4IV ACGT 2 cut(s) 525, 534
HpyCH4V TGCA 1 cut(s) 66
HpyF3I CTNAG 1 cut(s) 257
HpySE526I ACGT 2 cut(s) 525, 534
Hsp92I GRCGYC 3 cut(s) 378, 525, 534
Hsp92II CATG 4 cut(s) 35, 60, 88, 493
HspAI GCGC 2 cut(s) 162, 226
Ksp22I TGATCA 1 cut(s) 606
Kzo9I GATC 4 cut(s) 327, 332, 601, 606
LmnI GCTCC 3 cut(s) 127, 233, 646
Lsp1109I GCAGC 1 cut(s) 142
LweI GCATC 1 cut(s) 483
MaeI CTAG 2 cut(s) 20, 122
MaeII ACGT 2 cut(s) 525, 534
MalI GATC 4 cut(s) 329, 334, 603, 608
MboI GATC 4 cut(s) 327, 332, 601, 606
MboII GAAGA 7 cut(s) 288, 327, 361, 423, 513, 583, 604
MflI RGATCY 2 cut(s) 332, 601
MhlI GDGCHC 3 cut(s) 147, 277, 651
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 110, 234, 466, 512
MluNI TGGCCA 1 cut(s) 207
MlyI GAGTC 1 cut(s) 364
MnlI CCTC 5 cut(s) 266, 298, 358, 404, 635
Mox20I TGGCCA 1 cut(s) 207
MscI TGGCCA 1 cut(s) 207
MseI TTAA 1 cut(s) 74
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 1 cut(s) 574
MspR9I CCNGG 1 cut(s) 301
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 1 cut(s) 301
MvnI CGCG 1 cut(s) 436
NcoI CCATGG 1 cut(s) 489
NdeII GATC 4 cut(s) 327, 332, 601, 606
NlaIII CATG 4 cut(s) 35, 60, 88, 493
PcsI WCGNNNNNNNCGW 2 cut(s) 531, 534
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 28, 479, 641
PkrI GCNGC 4 cut(s) 90, 132, 377, 438
PleI GAGTC 1 cut(s) 363
PpsI GAGTC 1 cut(s) 363
PshAI GACNNNNGTC 1 cut(s) 524
Psp124BI GAGCTC 1 cut(s) 651
Psp6I CCWGG 1 cut(s) 299
PspFI CCCAGC 1 cut(s) 264
PspGI CCWGG 1 cut(s) 299
PsuI RGATCY 2 cut(s) 332, 601
SacI GAGCTC 1 cut(s) 651
SaqAI TTAA 1 cut(s) 74
SatI GCNGC 4 cut(s) 89, 131, 376, 437
Sau3AI GATC 4 cut(s) 327, 332, 601, 606
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 1 cut(s) 301
SduI GDGCHC 3 cut(s) 147, 277, 651
SetI ASST 5 cut(s) 80, 528, 537, 627, 651
SfaNI GCATC 1 cut(s) 483
SmlI CTYRAG 2 cut(s) 146, 170
SmoI CTYRAG 2 cut(s) 146, 170
Sse9I AATT 4 cut(s) 110, 234, 466, 512
SsiI CCGC 4 cut(s) 88, 125, 375, 436
SspMI CTAG 2 cut(s) 20, 122
SstI GAGCTC 1 cut(s) 651
StyD4I CCNGG 1 cut(s) 299
StyI CCWWGG 1 cut(s) 489
TaaI ACNGT 3 cut(s) 187, 343, 459
TaiI ACGT 2 cut(s) 528, 537
TaqI TCGA 6 cut(s) 153, 477, 537, 547, 627, 633
TasI AATT 4 cut(s) 110, 234, 466, 512
TauI GCSGC 3 cut(s) 91, 378, 439
TfiI GAWTC 3 cut(s) 28, 479, 641
Tru1I TTAA 1 cut(s) 74
Tru9I TTAA 1 cut(s) 74
TscAI CASTG 2 cut(s) 144, 394
TseI GCWGC 1 cut(s) 130
TspDTI ATGAA 3 cut(s) 20, 45, 235
TspGWI ACGGA 2 cut(s) 513, 536
TspRI CASTG 2 cut(s) 144, 394
XagI CCTNNNNNAGG 1 cut(s) 170
XapI RAATTY 1 cut(s) 110
XbaI TCTAGA 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 240
XspI CTAG 2 cut(s) 20, 122
ZraI GACGTC 2 cut(s) 526, 535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.