Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0005789.1

Orthogroup ID

OG0005080

Physical Location & Seq

Genomic Coordinates Reverse (-)

33546 .. 35868

Gene Structure 2323 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1632 bp

atgtctccggcagttgcagtgacacttagtttaggtaatactgtgtgtgataattccgcaattgcaaccaatgtggagttcgcatggctaaagctagcaacagacccagcaaatctttcatcagataccactaaggtggtgcctttggagttgatttcaaatggaagtggcaatgatactaggaatgaaattagtgtggtaaccgtgccatcacaagacgataacagtgggggagctgatttgttgaagttgctgccaaagaatggacacagtttggttattaatgattctttggttaaagaaagtgaggaagaaatcttatcctttagatatgatactaatggaattgttagtgagcagttgctaacgttagagttggggtctgagataagcttgacagatgttgtggagatgggaaatactggcgagggtcagattgttgcaaaagctattgtcttggtcgaatctactatagggcaagtgccttctggtgaagtcattgtggcagcagtaactccagtttctgagttacctggtaaaactgaattagcagcctctacagcagtggatttgcagtcaaacggggagaagaatgttagcaaagcagccatccgaagtgtttttgagctggactgtattcctctttggggttccgtttcaatctgtggaagaagaccagaaatggaagatgcaattgctgctgtgcctcggtttattaatattccaattaaaatgcttattggcaatcatgtgtacaatggaatgagcccaagtttgacccacctaaccagtcatttttttggtatttatgatgggcatggaggatctcaggttgctaactattgttgtgaacgccttcattcagctttggctgaagagcttcaaattattgaggatgatttgactgatggaataatgggagaaactcagcaggtgaagtgggagaaagcattcactaattgcttccagagtgtagatgatgagataggaggaacagtcagcagaggcatcaccggaagtaatgaggatgcttccggctccagctttgagcctgttgccgcagaaactgtggggtctacagctgtggttgcattagtttgttcttcccatattatagttgcaaactgtggtgattcaagagccgttctttgtcgtggcaaacaaccagtaccattatcagtagatcataaaccaaacagagaagatgaatatgcaagaattgaggcttctggagggaaggtcatacagtggaatggacatcgtgtatttggagttcttgcaatgtcgaggtccattggggatagatatttgaaaccctggataattccagatccagaagtcatgattgtccctagagccagagacgatgaattcctcattttggctagtgatggattatgggatgtcatgacaaacgaggaagcatgtgaagtggctcgaaggcgcatactgctgtggcacaaaaagaacggggttactcctctggcggaaagaggcacaggagttgatccagcagcgcaggaagcggcttcatatctttcaactcttgccctccaaaaaggaagcagagacaatatctctgtgattctggtggacttgaaagctcatagaaagttcaagtgcaaatcttga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003824 GO:0004721 GO:0004722 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005829 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0007275 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009787 GO:0009788 GO:0009966 GO:0009968 GO:0009987 GO:0010035 GO:0010646 GO:0010648 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019538 GO:0022622 GO:0023051 GO:0023057 GO:0032501 GO:0032502 GO:0036211 GO:0042221 GO:0042578 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044444 GO:0044464 GO:0048364 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0050896 GO:0065007 GO:0071704 GO:0099402 GO:0140096 GO:1901419 GO:1901420 GO:1901564 GO:1901700 GO:1905957 GO:1905958

KEGG Pathways

Metabolic & Signaling

ko04016 ko04075 ko04931 map04016 map04075 map04931

Pfam Domains

Protein Families

PP2C

Protein Analysis

543

Amino Acids

58.23

Weight (kDa)

4.85

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	240 - 526	4.6e-63	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005080)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G17550 AT1G17550 AT1G72770 AT1G72770 AT1G72770 AT1G72770 AT1G72770
fragaria_vesca	FvH4_6g34170 FvH4_6g34170 FvH4_6g34170 FvH4_6g34170
malus_domestica	MD09G1188000.v1.1 MD17G1172400.v1.1
prunus_persica	Prupe.3G044900_v2.0.a1 Prupe.3G044900_v2.0.a1 Prupe.3G044900_v2.0.a1
pyrus_communis	pycom09g10440 pycom17g17010
rosa_chinensis	RchiOBHm_Chr2g0144521
rosa_laevigata	RLG00000020085
rosa_multiflora	Rmu_sc0005789.1_g000006 Rmu_sc0009947.1_g000001
rosa_roxburghii	Rroxscaffold_2G00101240
rosa_rugosa	Rorug02G0387400 Rorug02G0387500
rosa_samantha	Rh2AG439500 Rh2BG449600 Rh2CG426500 Rh2DG459500
rosa_wichuraiana	Rw2G035950

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	922
Acc36I	ACCTGC	1 cut(s)	922
AccB1I	GGYRCC	1 cut(s)	139
AccB7I	CCANNNNNTGG	1 cut(s)	263
AccI	GTMKAC	1 cut(s)	1076
AciI	CCGC	4 cut(s)	57, 1059, 1487, 1526
AclI	AACGTT	1 cut(s)	368
AclWI	GGATC	3 cut(s)	832, 1325, 1502
AcsI	RAATTY	1 cut(s)	1370
AcuI	CTGAAG	1 cut(s)	894
AfaI	GTAC	2 cut(s)	755, 1170
AfiI	CCNNNNNNNGG	3 cut(s)	263, 647, 1381
AgsI	TTSAA	9 cut(s)	159, 247, 660, 884, 1137, 1312, 1542, 1600, 1618
AjnI	CCWGG	2 cut(s)	532, 1316
AjuI	GAANNNNNNNTTGG	2 cut(s)	62, 94
AleI	CACNNNNGTG	1 cut(s)	134
Alw26I	GTCTC	3 cut(s)	9, 1356, 1563
AlwI	GGATC	3 cut(s)	832, 1325, 1502
AlwNI	CAGNNNCTG	2 cut(s)	524, 1067
ApeKI	GCWGC	6 cut(s)	253, 506, 551, 605, 698, 1514
ApoI	RAATTY	1 cut(s)	1370
AseI	ATTAAT	2 cut(s)	282, 717
Asp700I	GAANNNNTTC	3 cut(s)	855, 879, 950
AspLEI	GCGC	2 cut(s)	1446, 1519
AspS9I	GGNCC	1 cut(s)	1290
AsuHPI	GGTGA	4 cut(s)	503, 946, 1003, 1142
AsuNHI	GCTAGC	1 cut(s)	94
AvaII	GGWCC	1 cut(s)	1290
BanI	GGYRCC	1 cut(s)	139
BanII	GRGCYC	1 cut(s)	770
BbsI	GAAGAC	1 cut(s)	679
BbvI	GCAGC	6 cut(s)	240, 518, 563, 617, 685, 1526
BccI	CCATC	6 cut(s)	217, 406, 617, 806, 902, 1385
BceAI	ACGGC	1 cut(s)	1127
BciT130I	CCWGG	2 cut(s)	534, 1318
BcoDI	GTCTC	3 cut(s)	9, 1356, 1563
BfaI	CTAG	4 cut(s)	95, 180, 1353, 1386
BfmI	CTRYAG	3 cut(s)	471, 558, 1077
BfuAI	ACCTGC	1 cut(s)	922
BisI	GCNGC	8 cut(s)	254, 507, 552, 606, 699, 1059, 1515, 1527
BlsI	GCNGC	8 cut(s)	255, 508, 553, 607, 700, 1060, 1516, 1528
Bme1390I	CCNGG	2 cut(s)	534, 1318
Bme18I	GGWCC	1 cut(s)	1290
BmgT120I	GGNCC	1 cut(s)	1290
BmiI	GGNNCC	3 cut(s)	141, 652, 1039
BmrFI	CCNGG	2 cut(s)	534, 1318
BmsI	GCATC	3 cut(s)	679, 1017, 1018
BmtI	GCTAGC	1 cut(s)	98
BpiI	GAAGAC	1 cut(s)	679
BpmI	CTGGAG	3 cut(s)	501, 1024, 1251
BsaJI	CCNNGG	2 cut(s)	707, 1316
BsaWI	WCCGGW	1 cut(s)	1013
BsaXI	ACNNNNNCTCC	4 cut(s)	981, 1011, 1224, 1254
Bsc4I	CCNNNNNNNGG	3 cut(s)	263, 647, 1381
Bse1I	ACTGG	4 cut(s)	427, 518, 789, 1166
Bse3DI	GCAATG	2 cut(s)	178, 1287
BseBI	CCWGG	2 cut(s)	534, 1318
BseDI	CCNNGG	2 cut(s)	707, 1316
BseGI	GGATG	4 cut(s)	609, 901, 1033, 1408
BseLI	CCNNNNNNNGG	3 cut(s)	263, 647, 1381
BseMI	GCAATG	2 cut(s)	178, 1287
BseMII	CTCAG	4 cut(s)	375, 516, 842, 941
BseNI	ACTGG	4 cut(s)	427, 518, 789, 1166
BseRI	GAGGAG	1 cut(s)	1470
BseXI	GCAGC	6 cut(s)	240, 518, 563, 617, 685, 1526
BseYI	CCCAGC	1 cut(s)	106
BshNI	GGYRCC	1 cut(s)	139
BsiSI	CCGG	3 cut(s)	8, 1014, 1035
BslFI	GGGAC	1 cut(s)	1334
BslI	CCNNNNNNNGG	3 cut(s)	263, 647, 1381
BsmAI	GTCTC	3 cut(s)	9, 1356, 1563
BsmBI	CGTCTC	1 cut(s)	1356
BsmFI	GGGAC	1 cut(s)	1334
BsmI	GAATGC	1 cut(s)	950
Bsp1286I	GDGCHC	1 cut(s)	770
Bsp1407I	TGTACA	1 cut(s)	753
Bsp143I	GATC	4 cut(s)	824, 1183, 1330, 1507
BspACI	CCGC	4 cut(s)	57, 1059, 1487, 1526
BspCNI	CTCAG	4 cut(s)	376, 517, 841, 940
BspHI	TCATGA	2 cut(s)	1341, 1407
BspLI	GGNNCC	3 cut(s)	141, 652, 1039
BspMI	ACCTGC	1 cut(s)	922
BspOI	GCTAGC	1 cut(s)	98
BspPI	GGATC	3 cut(s)	832, 1325, 1502
BspQI	GCTCTTC	1 cut(s)	870
BspT107I	GGYRCC	1 cut(s)	139
BsrDI	GCAATG	2 cut(s)	178, 1287
BsrGI	TGTACA	1 cut(s)	753
BsrI	ACTGG	4 cut(s)	427, 518, 789, 1166
BssECI	CCNNGG	2 cut(s)	707, 1316
BssMI	GATC	4 cut(s)	824, 1183, 1330, 1507
Bst2UI	CCWGG	2 cut(s)	534, 1318
Bst4CI	ACNGT	9 cut(s)	43, 205, 227, 272, 635, 997, 1069, 1127, 1248
Bst6I	CTCTTC	1 cut(s)	870
BstAPI	GCANNNNNTGC	1 cut(s)	698
BstAUI	TGTACA	1 cut(s)	753
BstC8I	GCNNGC	1 cut(s)	96
BstDEI	CTNAG	6 cut(s)	26, 132, 384, 525, 828, 927
BstEII	GGTNACC	1 cut(s)	199
BstF5I	GGATG	4 cut(s)	609, 901, 1033, 1408
BstHHI	GCGC	2 cut(s)	1446, 1519
BstKTI	GATC	4 cut(s)	827, 1186, 1333, 1510
BstMAI	GTCTC	3 cut(s)	9, 1356, 1563
BstMBI	GATC	4 cut(s)	824, 1183, 1330, 1507
BstMWI	GCNNNNNNNGC	5 cut(s)	560, 698, 1088, 1450, 1523
BstNI	CCWGG	2 cut(s)	534, 1318
BstNSI	RCATGY	1 cut(s)	1428
BstPI	GGTNACC	1 cut(s)	199
BstSCI	CCNGG	2 cut(s)	532, 1316
BstSFI	CTRYAG	3 cut(s)	471, 558, 1077
BstV1I	GCAGC	6 cut(s)	240, 518, 563, 617, 685, 1526
BstV2I	GAAGAC	1 cut(s)	679
BstX2I	RGATCY	2 cut(s)	824, 1330
BstXI	CCANNNNNNTGG	1 cut(s)	136
BstYI	RGATCY	2 cut(s)	824, 1330
BtsCI	GGATG	4 cut(s)	609, 901, 1033, 1408
BtsI	GCAGTG	2 cut(s)	24, 570
BtsIMutI	CAGTG	4 cut(s)	24, 232, 570, 1253
BveI	ACCTGC	1 cut(s)	922
Cac8I	GCNNGC	1 cut(s)	96
CaiI	CAGNNNCTG	2 cut(s)	524, 1067
CciI	TCATGA	2 cut(s)	1341, 1407
CfoI	GCGC	2 cut(s)	1446, 1519
Cfr13I	GGNCC	1 cut(s)	1290
CsiI	ACCWGGT	1 cut(s)	532
Csp6I	GTAC	2 cut(s)	754, 1169
CspCI	CAANNNNNGTGG	2 cut(s)	54, 89
CviAII	CATG	6 cut(s)	84, 749, 818, 1342, 1408, 1425
CviQI	GTAC	2 cut(s)	754, 1169
DdeI	CTNAG	6 cut(s)	26, 132, 384, 525, 828, 927
DpnI	GATC	4 cut(s)	826, 1185, 1332, 1509
DpnII	GATC	4 cut(s)	824, 1183, 1330, 1507
Eam1104I	CTCTTC	1 cut(s)	870
EarI	CTCTTC	1 cut(s)	870
EciI	GGCGGA	1 cut(s)	1502
Eco24I	GRGCYC	1 cut(s)	770
Eco47I	GGWCC	1 cut(s)	1290
Eco57I	CTGAAG	1 cut(s)	894
Eco91I	GGTNACC	1 cut(s)	199
EcoO65I	GGTNACC	1 cut(s)	199
EcoRI	GAATTC	1 cut(s)	1370
EcoRII	CCWGG	2 cut(s)	532, 1316
EcoT38I	GRGCYC	1 cut(s)	770
Esp3I	CGTCTC	1 cut(s)	1356
FaeI	CATG	6 cut(s)	87, 752, 821, 1345, 1411, 1428
FaqI	GGGAC	1 cut(s)	1334
FatI	CATG	6 cut(s)	83, 748, 817, 1341, 1407, 1424
FblI	GTMKAC	1 cut(s)	1076
Fnu4HI	GCNGC	8 cut(s)	254, 507, 552, 606, 699, 1059, 1515, 1527
FokI	GGATG	4 cut(s)	596, 908, 1040, 1415
FriOI	GRGCYC	1 cut(s)	770
Fsp4HI	GCNGC	8 cut(s)	254, 507, 552, 606, 699, 1059, 1515, 1527
FspBI	CTAG	4 cut(s)	95, 180, 1353, 1386
GlaI	GCGC	2 cut(s)	1445, 1518
GluI	GCNGC	8 cut(s)	254, 507, 552, 606, 699, 1059, 1515, 1527
GsaI	CCCAGC	1 cut(s)	110
GsuI	CTGGAG	3 cut(s)	501, 1024, 1251
HapII	CCGG	3 cut(s)	8, 1014, 1035
HhaI	GCGC	2 cut(s)	1446, 1519
Hin1II	CATG	6 cut(s)	87, 752, 821, 1345, 1411, 1428
Hin6I	GCGC	2 cut(s)	1444, 1517
HinP1I	GCGC	2 cut(s)	1444, 1517
HindIII	AAGCTT	1 cut(s)	391
HinfI	GANTC	4 cut(s)	287, 464, 1133, 1585
HpaII	CCGG	3 cut(s)	8, 1014, 1035
HphI	GGTGA	4 cut(s)	503, 946, 1003, 1142
Hpy166II	GTNNAC	4 cut(s)	754, 851, 1077, 1594
Hpy188I	TCNGA	5 cut(s)	124, 385, 435, 526, 614
Hpy188III	TCNNGA	8 cut(s)	967, 1137, 1230, 1328, 1334, 1342, 1408, 1629
Hpy8I	GTNNAC	4 cut(s)	754, 851, 1077, 1594
HpyAV	CCTTC	4 cut(s)	495, 866, 1231, 1434
HpyCH4III	ACNGT	9 cut(s)	43, 205, 227, 272, 635, 997, 1069, 1127, 1248
HpyCH4IV	ACGT	1 cut(s)	368
HpyF10VI	GCNNNNNNNGC	5 cut(s)	560, 698, 1088, 1450, 1523
HpyF3I	CTNAG	6 cut(s)	26, 132, 384, 525, 828, 927
HpySE526I	ACGT	1 cut(s)	368
Hsp92II	CATG	6 cut(s)	87, 752, 821, 1345, 1411, 1428
HspAI	GCGC	2 cut(s)	1444, 1517
Kzo9I	GATC	4 cut(s)	824, 1183, 1330, 1507
LguI	GCTCTTC	1 cut(s)	870
LmnI	GCTCC	2 cut(s)	233, 1043
Lsp1109I	GCAGC	6 cut(s)	240, 518, 563, 617, 685, 1526
LweI	GCATC	3 cut(s)	679, 1017, 1018
MabI	ACCWGGT	1 cut(s)	532
MaeI	CTAG	4 cut(s)	95, 180, 1353, 1386
MaeII	ACGT	1 cut(s)	368
MaeIII	GTNAC	5 cut(s)	19, 199, 511, 528, 1474
MalI	GATC	4 cut(s)	826, 1185, 1332, 1509
MboI	GATC	4 cut(s)	824, 1183, 1330, 1507
MboII	GAAGA	8 cut(s)	323, 601, 681, 684, 698, 887, 1095, 1214
MfeI	CAATTG	2 cut(s)	60, 693
MflI	RGATCY	2 cut(s)	824, 1330
MhlI	GDGCHC	1 cut(s)	770
MroXI	GAANNNNTTC	3 cut(s)	855, 879, 950
MseI	TTAA	4 cut(s)	282, 297, 717, 729
MslI	CAYNNNNRTG	1 cut(s)	134
MspA1I	CMGCKG	1 cut(s)	1082
MspI	CCGG	3 cut(s)	8, 1014, 1035
MspR9I	CCNGG	2 cut(s)	534, 1318
MunI	CAATTG	2 cut(s)	60, 693
Mva1269I	GAATGC	1 cut(s)	950
MvaI	CCWGG	2 cut(s)	534, 1318
MwoI	GCNNNNNNNGC	5 cut(s)	560, 698, 1088, 1450, 1523
NdeII	GATC	4 cut(s)	824, 1183, 1330, 1507
NheI	GCTAGC	1 cut(s)	94
NlaIII	CATG	6 cut(s)	87, 752, 821, 1345, 1411, 1428
NlaIV	GGNNCC	3 cut(s)	141, 652, 1039
NmuCI	GTSAC	1 cut(s)	19
NspI	RCATGY	1 cut(s)	1428
OliI	CACNNNNGTG	1 cut(s)	134
PagI	TCATGA	2 cut(s)	1341, 1407
PaqCI	CACCTGC	1 cut(s)	922
PciSI	GCTCTTC	1 cut(s)	870
PctI	GAATGC	1 cut(s)	950
PdmI	GAANNNNTTC	3 cut(s)	855, 879, 950
PfeI	GAWTC	4 cut(s)	287, 464, 1133, 1585
PflMI	CCANNNNNTGG	1 cut(s)	263
PkrI	GCNGC	8 cut(s)	255, 508, 553, 607, 700, 1060, 1516, 1528
PshBI	ATTAAT	2 cut(s)	282, 717
Psp1406I	AACGTT	1 cut(s)	368
Psp6I	CCWGG	2 cut(s)	532, 1316
PspEI	GGTNACC	1 cut(s)	199
PspFI	CCCAGC	1 cut(s)	106
PspGI	CCWGG	2 cut(s)	532, 1316
PspN4I	GGNNCC	3 cut(s)	141, 652, 1039
PspPI	GGNCC	1 cut(s)	1290
PsrI	GAACNNNNNNTAC	2 cut(s)	1257, 1289
PstNI	CAGNNNCTG	2 cut(s)	524, 1067
PsuI	RGATCY	2 cut(s)	824, 1330
PvuII	CAGCTG	1 cut(s)	1082
RsaI	GTAC	2 cut(s)	755, 1170
RsaNI	GTAC	2 cut(s)	754, 1169
RseI	CAYNNNNRTG	1 cut(s)	134
SapI	GCTCTTC	1 cut(s)	870
SaqAI	TTAA	4 cut(s)	282, 297, 717, 729
SatI	GCNGC	8 cut(s)	254, 507, 552, 606, 699, 1059, 1515, 1527
Sau3AI	GATC	4 cut(s)	824, 1183, 1330, 1507
Sau96I	GGNCC	1 cut(s)	1290
ScrFI	CCNGG	2 cut(s)	534, 1318
SduI	GDGCHC	1 cut(s)	770
SexAI	ACCWGGT	1 cut(s)	532
SfaNI	GCATC	3 cut(s)	679, 1017, 1018
SfcI	CTRYAG	3 cut(s)	471, 558, 1077
SinI	GGWCC	1 cut(s)	1290
SmiMI	CAYNNNNRTG	1 cut(s)	134
SsiI	CCGC	4 cut(s)	57, 1059, 1487, 1526
SspI	AATATT	1 cut(s)	721
SspMI	CTAG	4 cut(s)	95, 180, 1353, 1386
StyD4I	CCNGG	2 cut(s)	532, 1316
TaaI	ACNGT	9 cut(s)	43, 205, 227, 272, 635, 997, 1069, 1127, 1248
TaiI	ACGT	1 cut(s)	371
TaqI	TCGA	3 cut(s)	462, 1286, 1438
TatI	WGTACW	1 cut(s)	753
TauI	GCSGC	2 cut(s)	1061, 1529
TfiI	GAWTC	4 cut(s)	287, 464, 1133, 1585
Tru1I	TTAA	4 cut(s)	282, 297, 717, 729
Tru9I	TTAA	4 cut(s)	282, 297, 717, 729
TscAI	CASTG	4 cut(s)	24, 232, 570, 1253
TseFI	GTSAC	1 cut(s)	19
TseI	GCWGC	6 cut(s)	253, 506, 551, 605, 698, 1514
Tsp45I	GTSAC	1 cut(s)	19
TspDTI	ATGAA	6 cut(s)	108, 201, 848, 1221, 1383, 1521
TspGWI	ACGGA	1 cut(s)	643
TspRI	CASTG	4 cut(s)	24, 232, 570, 1253
Van91I	CCANNNNNTGG	1 cut(s)	263
VpaK11BI	GGWCC	1 cut(s)	1290
VspI	ATTAAT	2 cut(s)	282, 717
XapI	RAATTY	1 cut(s)	1370
XceI	RCATGY	1 cut(s)	1428
XmiI	GTMKAC	1 cut(s)	1076
XmnI	GAANNNNTTC	3 cut(s)	855, 879, 950
XspI	CTAG	4 cut(s)	95, 180, 1353, 1386

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.