Rmu_sc0006244.1_g000002

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006244.1
OG0007963
Physical Location & Seq
Forward (+)
5658 .. 10966
5309 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1872 bp
atgcaagcctccctctacctggacaccaagactctcgcatggccttcacgtggccacacctcaacccaccacatattccggccaacacccacagtcagactcaggccattgcccccagtctccgccgtggccacgtcacctccgcctctaactcggcagaaggcccactcgttgccaccggagaaggtggagctcttcaagtcactagagggctgggcctcccagacaatcctcccatttctgaagccggtggacgaatgctggcaacccagtgccttcttgccggacccgtctctcccgaaggaggagttcttggacagggttcgggcacttaggaaccggaccgatgggctgcccgacgagtactttgtggtgctggtcggggatatgatcaccgaggaggcgttgcctacgtaccagagtatgatcaatgggctcgatggggtgagagacgagaccggagctagctccagcccgtgggcgcggtggactcgggcttggaccgctgaggaaaaccgccacggcgatctgcttaaaacctatctgtacttgtccggtcgggttgatatgttcaagatcgagaagactattcagaaccttattggagctggcatggagccagggacggagaataacccgtacttgggtttcgtgtacacttcatttcaagagcgagccacgttcatatcgcatggcaacacggctcggctggctaaggaccgcggcgatcctgtgcttgccagaatatgcggcaccattgcatccgacgagaggcgccacgagttcgcctattccaagattgtggaaaagctactcgaagtggacccaaccggagcaatggtcgccattgcggacatgatgaggaagaagatcacaatgccggcccagcttatggacgatggggaggaccccaagctgttcgagcattttgcggccgtgactcaacggcttggggtgtacacggcacatgattatgcggacattttggagtttttgatcggacggtggaggctggagaaggtggaaggaatgacaggggagggtaaacgtgcacaggacttcgtgtgtgggttggcgccgaggatcaggaggctcgaagagcgggccgatgagcgagcaaggaagatggagccgcatggcgttaagtttagctggatttacaataaagaagtgggtttcgcggcaccaagcagcttggggctgaagctcccaatcgaacccgataccggtcgaagaacttgctggtgctgggatgatcaacaacccgttggcttaaattggggcggccgagttgttgagcgagacgaggcgttcagccggaagctattcttcttcttcttctctctcctcctctctctctctctcctctccttctcctctgcccaaaccatcactaacgccgccgagatcctctccaattcctcctacaaatccatctccctcgcactcctgtggattaaccacctcctcccatctctttccctgtctgatcactcaagtccctcccgttcggtgccaagatcgctaccatgctcgatggccactctatcacggtaccttcccgctaaacgcctcgtctcgctcaacaacttggcagcctcaatttacgccgacgggtcgttgatcatctcagcaccagcaggttcttcgaccgattccttgcaactcaccggtgagctcgaccgcttccaaccgcctcaccgatgcaggtcgacttttgccctgattctgctacagctcacccaagctcggctgacgctgacaacaacaaccacacgacgtcttcgatggcctcctggacacctcgccgtctctttggcattgccgatagcctcctggacgacgtcggtgcttgacccaggctgtggctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0016020
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

623

Amino Acids

69.6

Weight (kDa)

8.31

Isoelectric Point (pI)

43.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FA_desaturase_2 PF03405 61 - 386 5.6e-152 Fatty acid desaturase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 1783, 1847
Acc36I ACCTGC 2 cut(s) 1631, 1698
Acc65I GGTACC 1 cut(s) 1554
AccB1I GGYRCC 6 cut(s) 752, 774, 1075, 1183, 1513, 1554
AccB7I CCANNNNNTGG 3 cut(s) 477, 892, 1865
AccBSI CCGCTC 1 cut(s) 1102
AccI GTMKAC 1 cut(s) 1712
AccII CGCG 3 cut(s) 484, 723, 1181
AclWI GGATC 3 cut(s) 722, 1091, 1402
AcoI YGGCCR 6 cut(s) 52, 80, 129, 933, 1285, 1539
AcuI CTGAAG 2 cut(s) 263, 1223
AcvI CACGTG 1 cut(s) 50
AcyI GRCGYC 4 cut(s) 775, 1076, 1780, 1844
AfaI GTAC 7 cut(s) 365, 416, 548, 641, 656, 959, 1556
AgeI ACCGGT 2 cut(s) 1226, 1670
AgsI TTSAA 3 cut(s) 199, 574, 668
AjiI CACGTC 1 cut(s) 135
AjnI CCWGG 5 cut(s) 18, 619, 1795, 1835, 1858
AleI CACNNNNGTG 1 cut(s) 1450
Alw21I GWGCWC 3 cut(s) 195, 1054, 1680
Alw26I GTCTC 7 cut(s) 124, 297, 444, 449, 1296, 1582, 1816
Alw44I GTGCAC 1 cut(s) 1050
AlwI GGATC 3 cut(s) 722, 1091, 1402
Ama87I CYCGRG 1 cut(s) 492
ApaLI GTGCAC 1 cut(s) 1050
ApeKI GCWGC 3 cut(s) 352, 1191, 1595
ArsI GACNNNNNNTTYG 2 cut(s) 350, 382
AsiGI ACCGGT 2 cut(s) 1226, 1670
Asp700I GAANNNNTTC 1 cut(s) 1325
Asp718I GGTACC 1 cut(s) 1554
AspLEI GCGC 3 cut(s) 484, 777, 1078
AsuHPI GGTGA 7 cut(s) 129, 385, 457, 1660, 1685, 1691, 1732
AsuNHI GCTAGC 1 cut(s) 464
AvaI CYCGRG 1 cut(s) 492
AvaII GGWCC 6 cut(s) 286, 342, 501, 718, 823, 907
BaeGI GKGCMC 2 cut(s) 331, 1054
BalI TGGCCA 3 cut(s) 54, 131, 1541
BanI GGYRCC 6 cut(s) 752, 774, 1075, 1183, 1513, 1554
BanII GRGCYC 3 cut(s) 195, 438, 1680
BauI CACGAG 1 cut(s) 779
BbrPI CACGTG 1 cut(s) 50
BbsI GAAGAC 2 cut(s) 590, 1775
Bbv12I GWGCWC 3 cut(s) 195, 1054, 1680
BbvCI CCTCAGC 1 cut(s) 507
BbvI GCAGC 3 cut(s) 339, 1203, 1607
BccI CCATC 9 cut(s) 341, 434, 893, 1120, 1397, 1442, 1480, 1531, 1782
BceAI ACGGC 7 cut(s) 110, 538, 717, 920, 962, 978, 1793
BcgI CGANNNNNNTGC 8 cut(s) 911, 945, 1221, 1255, 1629, 1663, 1831, 1865
BciT130I CCWGG 5 cut(s) 20, 621, 1797, 1837, 1860
BclI TGATCA 5 cut(s) 390, 426, 1255, 1489, 1623
BcoDI GTCTC 7 cut(s) 124, 297, 444, 449, 1296, 1582, 1816
BfaI CTAG 2 cut(s) 206, 465
BfmI CTRYAG 1 cut(s) 1733
BfoI RGCGCY 2 cut(s) 778, 1079
BfuAI ACCTGC 2 cut(s) 1631, 1698
BmcAI AGTACT 1 cut(s) 365
Bme1390I CCNGG 5 cut(s) 20, 621, 1797, 1837, 1860
Bme18I GGWCC 6 cut(s) 286, 342, 501, 718, 823, 907
BmeT110I CYCGRG 1 cut(s) 492
BmgBI CACGTC 1 cut(s) 135
BmrFI CCNGG 5 cut(s) 20, 621, 1797, 1837, 1860
BmrI ACTGGG 2 cut(s) 110, 264
BmsI GCATC 2 cut(s) 770, 1694
BmtI GCTAGC 1 cut(s) 468
BmuI ACTGGG 2 cut(s) 110, 264
BpiI GAAGAC 2 cut(s) 590, 1775
BplI GAGNNNNNCTC 2 cut(s) 1397, 1429
BpmI CTGGAG 2 cut(s) 454, 1034
Bpu10I CCTNAGC 2 cut(s) 507, 714
BpuEI CTTGAG 1 cut(s) 1480
BsaAI YACGTR 2 cut(s) 50, 414
BsaHI GRCGYC 4 cut(s) 775, 1076, 1780, 1844
BsaI GGTCTC 1 cut(s) 449
BsaJI CCNNGG 8 cut(s) 126, 396, 476, 520, 620, 721, 1079, 1858
BsaWI WCCGGW 7 cut(s) 178, 339, 458, 554, 830, 1226, 1670
BsaXI ACNNNNNCTCC 4 cut(s) 124, 154, 825, 855
Bse118I RCCGGY 4 cut(s) 247, 880, 1226, 1670
Bse1I ACTGG 2 cut(s) 116, 270
Bse3DI GCAATG 5 cut(s) 107, 756, 843, 846, 1820
BseBI CCWGG 5 cut(s) 20, 621, 1797, 1837, 1860
BseDI CCNNGG 8 cut(s) 126, 396, 476, 520, 620, 721, 1079, 1858
BseGI GGATG 2 cut(s) 761, 1258
BseMI GCAATG 5 cut(s) 107, 756, 843, 846, 1820
BseMII CTCAG 3 cut(s) 115, 498, 1644
BseNI ACTGG 2 cut(s) 116, 270
BseRI GAGGAG 7 cut(s) 320, 413, 1337, 1340, 1355, 1366, 1457
BseSI GKGCMC 2 cut(s) 331, 1054
BseX3I CGGCCG 2 cut(s) 933, 1285
BseXI GCAGC 3 cut(s) 339, 1203, 1607
BseYI CCCAGC 3 cut(s) 213, 885, 1248
Bsh1236I CGCG 3 cut(s) 484, 723, 1181
Bsh1285I CGRYCG 6 cut(s) 559, 936, 1231, 1288, 1653, 1684
BshNI GGYRCC 6 cut(s) 752, 774, 1075, 1183, 1513, 1554
BshTI ACCGGT 2 cut(s) 1226, 1670
BsiEI CGRYCG 6 cut(s) 559, 936, 1231, 1288, 1653, 1684
BsiHKAI GWGCWC 3 cut(s) 195, 1054, 1680
BsiHKCI CYCGRG 1 cut(s) 492
BslFI GGGAC 2 cut(s) 637, 1485
BsmAI GTCTC 7 cut(s) 124, 297, 444, 449, 1296, 1582, 1816
BsmBI CGTCTC 5 cut(s) 297, 444, 1296, 1582, 1816
BsmFI GGGAC 2 cut(s) 637, 1485
BsmI GAATGC 1 cut(s) 263
Bso31I GGTCTC 1 cut(s) 449
BsoBI CYCGRG 1 cut(s) 492
Bsp1286I GDGCHC 5 cut(s) 195, 331, 438, 1054, 1680
Bsp1407I TGTACA 2 cut(s) 654, 957
BspCNI CTCAG 3 cut(s) 114, 499, 1643
BspFNI CGCG 3 cut(s) 484, 723, 1181
BspMI ACCTGC 2 cut(s) 1631, 1698
BspOI GCTAGC 1 cut(s) 468
BspPI GGATC 3 cut(s) 722, 1091, 1402
BspQI GCTCTTC 2 cut(s) 200, 1092
BspT107I GGYRCC 6 cut(s) 752, 774, 1075, 1183, 1513, 1554
BspTNI GGTCTC 1 cut(s) 449
BsrBI CCGCTC 1 cut(s) 1102
BsrDI GCAATG 5 cut(s) 107, 756, 843, 846, 1820
BsrFI RCCGGY 4 cut(s) 247, 880, 1226, 1670
BsrGI TGTACA 2 cut(s) 654, 957
BsrI ACTGG 2 cut(s) 116, 270
BssAI RCCGGY 4 cut(s) 247, 880, 1226, 1670
BssECI CCNNGG 8 cut(s) 126, 396, 476, 520, 620, 721, 1079, 1858
BssNI GRCGYC 4 cut(s) 775, 1076, 1780, 1844
BssSI CACGAG 1 cut(s) 779
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 5 cut(s) 20, 621, 1797, 1837, 1860
Bst4CI ACNGT 3 cut(s) 94, 1005, 1554
Bst6I CTCTTC 2 cut(s) 200, 1092
BstACI GRCGYC 4 cut(s) 775, 1076, 1780, 1844
BstAUI TGTACA 2 cut(s) 654, 957
BstBAI YACGTR 2 cut(s) 50, 414
BstDEI CTNAG 5 cut(s) 101, 332, 507, 714, 1630
BstDSI CCRYGG 4 cut(s) 126, 476, 520, 721
BstF5I GGATG 2 cut(s) 761, 1258
BstFNI CGCG 3 cut(s) 484, 723, 1181
BstH2I RGCGCY 2 cut(s) 778, 1079
BstHHI GCGC 3 cut(s) 484, 777, 1078
BstMAI GTCTC 7 cut(s) 124, 297, 444, 449, 1296, 1582, 1816
BstMCI CGRYCG 6 cut(s) 559, 936, 1231, 1288, 1653, 1684
BstMWI GCNNNNNNNGC 7 cut(s) 503, 710, 842, 886, 922, 1099, 1522
BstNI CCWGG 5 cut(s) 20, 621, 1797, 1837, 1860
BstSCI CCNGG 5 cut(s) 18, 619, 1795, 1835, 1858
BstSFI CTRYAG 1 cut(s) 1733
BstSLI GKGCMC 2 cut(s) 331, 1054
BstSNI TACGTA 1 cut(s) 414
BstUI CGCG 3 cut(s) 484, 723, 1181
BstV1I GCAGC 3 cut(s) 339, 1203, 1607
BstV2I GAAGAC 2 cut(s) 590, 1775
BstX2I RGATCY 1 cut(s) 1407
BstXI CCANNNNNNTGG 1 cut(s) 802
BstYI RGATCY 1 cut(s) 1407
BstZI CGGCCG 2 cut(s) 933, 1285
BtgI CCRYGG 4 cut(s) 126, 476, 520, 721
BtrI CACGTC 1 cut(s) 135
BtsCI GGATG 2 cut(s) 761, 1258
BtsIMutI CAGTG 1 cut(s) 277
BveI ACCTGC 2 cut(s) 1631, 1698
CfoI GCGC 3 cut(s) 484, 777, 1078
Cfr10I RCCGGY 4 cut(s) 247, 880, 1226, 1670
Cfr42I CCGCGG 1 cut(s) 724
CpoI CGGWCCG 1 cut(s) 342
CseI GACGC 1 cut(s) 1765
Csp6I GTAC 7 cut(s) 364, 415, 547, 640, 655, 958, 1555
CspAI ACCGGT 2 cut(s) 1226, 1670
CspI CGGWCCG 1 cut(s) 342
CviAII CATG 7 cut(s) 39, 613, 692, 856, 968, 1136, 1530
CviQI GTAC 7 cut(s) 364, 415, 547, 640, 655, 958, 1555
DdeI CTNAG 5 cut(s) 101, 332, 507, 714, 1630
DinI GGCGCC 2 cut(s) 776, 1077
EaeI YGGCCR 6 cut(s) 52, 80, 129, 933, 1285, 1539
EagI CGGCCG 2 cut(s) 933, 1285
Eam1104I CTCTTC 2 cut(s) 200, 1092
EarI CTCTTC 2 cut(s) 200, 1092
EciI GGCGGA 2 cut(s) 112, 132
Ecl136II GAGCTC 2 cut(s) 193, 1678
EclXI CGGCCG 2 cut(s) 933, 1285
Eco105I TACGTA 1 cut(s) 414
Eco24I GRGCYC 3 cut(s) 195, 438, 1680
Eco31I GGTCTC 1 cut(s) 449
Eco47I GGWCC 6 cut(s) 286, 342, 501, 718, 823, 907
Eco52I CGGCCG 2 cut(s) 933, 1285
Eco53kI GAGCTC 2 cut(s) 193, 1678
Eco57I CTGAAG 2 cut(s) 263, 1223
Eco72I CACGTG 1 cut(s) 50
Eco88I CYCGRG 1 cut(s) 492
EcoICRI GAGCTC 2 cut(s) 193, 1678
EcoO109I RGGNCCY 1 cut(s) 907
EcoRII CCWGG 5 cut(s) 18, 619, 1795, 1835, 1858
EcoT38I GRGCYC 3 cut(s) 195, 438, 1680
EgeI GGCGCC 2 cut(s) 776, 1077
EheI GGCGCC 2 cut(s) 776, 1077
Esp3I CGTCTC 5 cut(s) 297, 444, 1296, 1582, 1816
FaeI CATG 7 cut(s) 42, 616, 695, 859, 971, 1139, 1533
FalI AAGNNNNNCTT 2 cut(s) 1313, 1345
FaqI GGGAC 2 cut(s) 637, 1485
FatI CATG 7 cut(s) 38, 612, 691, 855, 967, 1135, 1529
FauI CCCGC 2 cut(s) 1095, 1570
FbaI TGATCA 5 cut(s) 390, 426, 1255, 1489, 1623
FblI GTMKAC 1 cut(s) 1712
FokI GGATG 2 cut(s) 748, 1265
FriOI GRGCYC 3 cut(s) 195, 438, 1680
FspBI CTAG 2 cut(s) 206, 465
GlaI GCGC 3 cut(s) 483, 776, 1077
GsaI CCCAGC 3 cut(s) 217, 889, 1252
GsuI CTGGAG 2 cut(s) 454, 1034
HaeII RGCGCY 2 cut(s) 778, 1079
HgaI GACGC 1 cut(s) 1765
HhaI GCGC 3 cut(s) 484, 777, 1078
Hin1I GRCGYC 4 cut(s) 775, 1076, 1780, 1844
Hin1II CATG 7 cut(s) 42, 616, 695, 859, 971, 1139, 1533
Hin6I GCGC 3 cut(s) 482, 775, 1076
HinP1I GCGC 3 cut(s) 482, 775, 1076
HincII GTYRAC 1 cut(s) 1713
HindII GTYRAC 1 cut(s) 1713
HinfI GANTC 6 cut(s) 31, 99, 490, 940, 1655, 1726
HphI GGTGA 7 cut(s) 129, 385, 457, 1660, 1685, 1691, 1732
Hpy188I TCNGA 6 cut(s) 98, 243, 594, 766, 1001, 1489
Hpy188III TCNNGA 5 cut(s) 298, 574, 580, 668, 1087
Hpy99I CGWCG 6 cut(s) 362, 770, 1616, 1782, 1846, 1849
HpyAV CCTTC 9 cut(s) 54, 154, 178, 286, 295, 1012, 1019, 1381, 1568
HpyCH4III ACNGT 3 cut(s) 94, 1005, 1554
HpyCH4IV ACGT 7 cut(s) 49, 134, 413, 680, 1048, 1780, 1844
HpyCH4V TGCA 5 cut(s) 4, 761, 1052, 1663, 1707
HpyF10VI GCNNNNNNNGC 7 cut(s) 503, 710, 842, 886, 922, 1099, 1522
HpyF3I CTNAG 5 cut(s) 101, 332, 507, 714, 1630
HpySE526I ACGT 7 cut(s) 49, 134, 413, 680, 1048, 1780, 1844
Hsp92I GRCGYC 4 cut(s) 775, 1076, 1780, 1844
Hsp92II CATG 7 cut(s) 42, 616, 695, 859, 971, 1139, 1533
HspAI GCGC 3 cut(s) 482, 775, 1076
KasI GGCGCC 2 cut(s) 774, 1075
KpnI GGTACC 1 cut(s) 1558
KroI GCCGGC 1 cut(s) 880
KroNI GCCGGC 1 cut(s) 882
Ksp22I TGATCA 5 cut(s) 390, 426, 1255, 1489, 1623
KspI CCGCGG 1 cut(s) 724
LguI GCTCTTC 2 cut(s) 200, 1092
LmnI GCTCC 8 cut(s) 190, 461, 473, 605, 616, 833, 1129, 1212
Lsp1109I GCAGC 3 cut(s) 339, 1203, 1607
LweI GCATC 2 cut(s) 770, 1694
MaeI CTAG 2 cut(s) 206, 465
MaeII ACGT 7 cut(s) 49, 134, 413, 680, 1048, 1780, 1844
MaeIII GTNAC 3 cut(s) 135, 201, 937
MbiI CCGCTC 1 cut(s) 1102
MflI RGATCY 1 cut(s) 1407
MhlI GDGCHC 5 cut(s) 195, 331, 438, 1054, 1680
MlsI TGGCCA 3 cut(s) 54, 131, 1541
MluCI AATT 3 cut(s) 1276, 1417, 1602
MluNI TGGCCA 3 cut(s) 54, 131, 1541
Mly113I GGCGCC 2 cut(s) 775, 1076
MlyI GAGTC 4 cut(s) 25, 93, 484, 934
MmeI TCCRAC 2 cut(s) 789, 1714
Mox20I TGGCCA 3 cut(s) 54, 131, 1541
MroNI GCCGGC 1 cut(s) 880
MroXI GAANNNNTTC 1 cut(s) 1325
MscI TGGCCA 3 cut(s) 54, 131, 1541
MseI TTAA 4 cut(s) 534, 1143, 1274, 1458
MslI CAYNNNNRTG 2 cut(s) 972, 1450
Msp20I TGGCCA 3 cut(s) 54, 131, 1541
MspA1I CMGCKG 2 cut(s) 506, 723
MspR9I CCNGG 5 cut(s) 20, 621, 1797, 1837, 1860
Mva1269I GAATGC 1 cut(s) 263
MvaI CCWGG 5 cut(s) 20, 621, 1797, 1837, 1860
MvnI CGCG 3 cut(s) 484, 723, 1181
MwoI GCNNNNNNNGC 7 cut(s) 503, 710, 842, 886, 922, 1099, 1522
NaeI GCCGGC 1 cut(s) 882
NarI GGCGCC 2 cut(s) 775, 1076
NgoMIV GCCGGC 1 cut(s) 880
NheI GCTAGC 1 cut(s) 464
NlaIII CATG 7 cut(s) 42, 616, 695, 859, 971, 1139, 1533
NmeAIII GCCGAG 6 cut(s) 133, 685, 1104, 1313, 1429, 1729
NmuCI GTSAC 3 cut(s) 135, 201, 937
OliI CACNNNNGTG 1 cut(s) 1450
PciSI GCTCTTC 2 cut(s) 200, 1092
PcsI WCGNNNNNNNCGW 1 cut(s) 1783
PctI GAATGC 1 cut(s) 263
PdiI GCCGGC 1 cut(s) 882
PdmI GAANNNNTTC 1 cut(s) 1325
PfeI GAWTC 2 cut(s) 1655, 1726
PflFI GACNNNGTC 1 cut(s) 1843
PflMI CCANNNNNTGG 3 cut(s) 477, 892, 1865
PfoI TCCNGGA 2 cut(s) 1795, 1835
PinAI ACCGGT 2 cut(s) 1226, 1670
PleI GAGTC 4 cut(s) 25, 93, 484, 934
PluTI GGCGCC 2 cut(s) 778, 1079
PmaCI CACGTG 1 cut(s) 50
PmlI CACGTG 1 cut(s) 50
PpsI GAGTC 4 cut(s) 25, 93, 484, 934
Ppu21I YACGTR 2 cut(s) 50, 414
PpuMI RGGWCCY 1 cut(s) 907
Psp124BI GAGCTC 2 cut(s) 195, 1680
Psp5II RGGWCCY 1 cut(s) 907
Psp6I CCWGG 5 cut(s) 18, 619, 1795, 1835, 1858
PspCI CACGTG 1 cut(s) 50
PspFI CCCAGC 3 cut(s) 213, 885, 1248
PspGI CCWGG 5 cut(s) 18, 619, 1795, 1835, 1858
PspPPI RGGWCCY 1 cut(s) 907
PsuI RGATCY 1 cut(s) 1407
PsyI GACNNNGTC 1 cut(s) 1843
RsaI GTAC 7 cut(s) 365, 416, 548, 641, 656, 959, 1556
RsaNI GTAC 7 cut(s) 364, 415, 547, 640, 655, 958, 1555
RseI CAYNNNNRTG 2 cut(s) 972, 1450
Rsr2I CGGWCCG 1 cut(s) 342
RsrII CGGWCCG 1 cut(s) 342
SacI GAGCTC 2 cut(s) 195, 1680
SacII CCGCGG 1 cut(s) 724
SalI GTCGAC 1 cut(s) 1711
SapI GCTCTTC 2 cut(s) 200, 1092
SaqAI TTAA 4 cut(s) 534, 1143, 1274, 1458
ScaI AGTACT 1 cut(s) 365
SchI GAGTC 4 cut(s) 25, 93, 484, 934
ScrFI CCNGG 5 cut(s) 20, 621, 1797, 1837, 1860
SduI GDGCHC 5 cut(s) 195, 331, 438, 1054, 1680
SfaNI GCATC 2 cut(s) 770, 1694
SfcI CTRYAG 1 cut(s) 1733
SfoI GGCGCC 2 cut(s) 776, 1077
Sfr303I CCGCGG 1 cut(s) 724
SgrAI CRCCGGYG 1 cut(s) 1670
SgrBI CCGCGG 1 cut(s) 724
SinI GGWCC 6 cut(s) 286, 342, 501, 718, 823, 907
SmiMI CAYNNNNRTG 2 cut(s) 972, 1450
SmlI CTYRAG 1 cut(s) 1495
SmoI CTYRAG 1 cut(s) 1495
SnaBI TACGTA 1 cut(s) 414
Sse9I AATT 3 cut(s) 1276, 1417, 1602
SspDI GGCGCC 2 cut(s) 774, 1075
SspMI CTAG 2 cut(s) 206, 465
SstI GAGCTC 2 cut(s) 195, 1680
StyD4I CCNGG 5 cut(s) 18, 619, 1795, 1835, 1858
TaaI ACNGT 3 cut(s) 94, 1005, 1554
TaiI ACGT 7 cut(s) 52, 137, 416, 683, 1051, 1783, 1847
TaqII GACCGA 2 cut(s) 359, 1667
TasI AATT 3 cut(s) 1276, 1417, 1602
TatI WGTACW 4 cut(s) 363, 546, 654, 957
TauI GCSGC 7 cut(s) 726, 753, 935, 1135, 1184, 1287, 1403
TfiI GAWTC 2 cut(s) 1655, 1726
Tru1I TTAA 4 cut(s) 534, 1143, 1274, 1458
Tru9I TTAA 4 cut(s) 534, 1143, 1274, 1458
TscAI CASTG 1 cut(s) 277
TseFI GTSAC 3 cut(s) 135, 201, 937
TseI GCWGC 3 cut(s) 352, 1191, 1595
Tsp45I GTSAC 3 cut(s) 135, 201, 937
TspDTI ATGAA 2 cut(s) 651, 673
TspGWI ACGGA 1 cut(s) 641
TspRI CASTG 1 cut(s) 277
Tth111I GACNNNGTC 1 cut(s) 1843
Van91I CCANNNNNTGG 3 cut(s) 477, 892, 1865
VneI GTGCAC 1 cut(s) 1050
VpaK11BI GGWCC 6 cut(s) 286, 342, 501, 718, 823, 907
XmiI GTMKAC 1 cut(s) 1712
XmnI GAANNNNTTC 1 cut(s) 1325
XspI CTAG 2 cut(s) 206, 465
ZraI GACGTC 2 cut(s) 1781, 1845
ZrmI AGTACT 1 cut(s) 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.