Rmu_sc0006297.1_g000001

Protein ROOT PRIMORDIUM DEFECTIVE 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006297.1
OG0011284
Physical Location & Seq
Reverse (-)
2 .. 641
640 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 640 bp
atgcatgattctttggtaagctatagtatagtcaagaatgctgctcatctactgcgtggggagaaagtcttcacttcaggttcgcaagacacagaatgtaatgacaggcaagaatgctgcttatctatttaccaagacagaaccatggttccgtacatcctatttagtaaggttggggttggcttcatccgtggaacacaagtatttaatttccagcacaagcgtactctggtgaatattagattgaaatgggttaaagatagggcactggatgctgttgtgactgctgagagggatctcaaagctgcctgtgttcttgtttccattatctcctcctcgtctgatggctgcctccccatataccatctctcttgtcatcgtggacagctcgatcttccttatgacctcaaggtctctacttttatcaggaggtacccatctgtttttctagagtcccatgttctcgatagcggtggcactcgtgtcccatgttttagcttaactcctgaagccataaaactccgccaggaagagtccaatgtcgttcagcagaataagattgatatccttaataggcttcggaaactgctcatgctcactaggggctggaagcttcctttacaaaccattgaccagctga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PORR

Protein Analysis

213

Amino Acids

24.25

Weight (kDa)

9.11

Isoelectric Point (pI)

50.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PORR PF11955 85 - 213 7.8e-29 Plant organelle RNA recognition domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 410
Acc65I GGTACC 1 cut(s) 432
AccB1I GGYRCC 1 cut(s) 432
AciI CCGC 2 cut(s) 471, 523
AclWI GGATC 1 cut(s) 303
AcuI CTGAAG 2 cut(s) 60, 528
AfaI GTAC 3 cut(s) 155, 226, 434
AgsI TTSAA 1 cut(s) 247
AjnI CCWGG 1 cut(s) 525
AloI GAACNNNNNNTCC 2 cut(s) 133, 165
AluBI AGCT 6 cut(s) 21, 305, 388, 498, 613, 637
AluI AGCT 6 cut(s) 21, 305, 388, 498, 613, 637
Alw26I GTCTC 1 cut(s) 418
AlwI GGATC 1 cut(s) 303
ApeKI GCWGC 4 cut(s) 41, 117, 305, 348
Asp700I GAANNNNTTC 1 cut(s) 68
Asp718I GGTACC 1 cut(s) 432
AsuHPI GGTGA 1 cut(s) 244
BaeGI GKGCMC 1 cut(s) 268
BanI GGYRCC 1 cut(s) 432
BauI CACGAG 1 cut(s) 480
BbsI GAAGAC 1 cut(s) 61
BbvI GCAGC 4 cut(s) 28, 104, 292, 335
BccI CCATC 3 cut(s) 338, 372, 445
BciT130I CCWGG 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 418
BfaI CTAG 2 cut(s) 449, 600
BfmI CTRYAG 1 cut(s) 22
BisI GCNGC 4 cut(s) 42, 118, 306, 349
BlsI GCNGC 4 cut(s) 43, 119, 307, 350
Bme1390I CCNGG 1 cut(s) 527
BmiI GGNNCC 2 cut(s) 150, 434
BmrFI CCNGG 1 cut(s) 527
BmsI GCATC 1 cut(s) 262
BpiI GAAGAC 1 cut(s) 61
BpuEI CTTGAG 1 cut(s) 392
BsaBI GATNNNNATC 1 cut(s) 563
BsaI GGTCTC 1 cut(s) 418
BsaJI CCNNGG 2 cut(s) 144, 190
Bse1I ACTGG 1 cut(s) 273
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 1 cut(s) 527
BseDI CCNNGG 2 cut(s) 144, 190
BseGI GGATG 3 cut(s) 156, 186, 277
BseJI GATNNNNATC 1 cut(s) 563
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 1 cut(s) 273
BseRI GAGGAG 2 cut(s) 322, 325
BseSI GKGCMC 1 cut(s) 268
BseXI GCAGC 4 cut(s) 28, 104, 292, 335
BshNI GGYRCC 1 cut(s) 432
BslFI GGGAC 2 cut(s) 439, 470
BsmAI GTCTC 1 cut(s) 418
BsmFI GGGAC 2 cut(s) 439, 470
BsmI GAATGC 2 cut(s) 43, 119
Bso31I GGTCTC 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 268
Bsp143I GATC 2 cut(s) 295, 391
Bsp19I CCATGG 1 cut(s) 144
BspACI CCGC 2 cut(s) 471, 523
BspCNI CTCAG 1 cut(s) 280
BspLI GGNNCC 2 cut(s) 150, 434
BspPI GGATC 1 cut(s) 303
BspT107I GGYRCC 1 cut(s) 432
BspTNI GGTCTC 1 cut(s) 418
BsrI ACTGG 1 cut(s) 273
BssECI CCNNGG 2 cut(s) 144, 190
BssMI GATC 2 cut(s) 295, 391
BssSI CACGAG 1 cut(s) 480
BssT1I CCWWGG 1 cut(s) 144
Bst2BI CACGAG 1 cut(s) 480
Bst2UI CCWGG 1 cut(s) 527
Bst6I CTCTTC 1 cut(s) 525
BstAPI GCANNNNNTGC 1 cut(s) 272
BstDEI CTNAG 1 cut(s) 288
BstDSI CCRYGG 2 cut(s) 144, 190
BstF5I GGATG 3 cut(s) 156, 186, 277
BstKTI GATC 2 cut(s) 298, 394
BstMAI GTCTC 1 cut(s) 418
BstMBI GATC 2 cut(s) 295, 391
BstMWI GCNNNNNNNGC 1 cut(s) 272
BstNI CCWGG 1 cut(s) 527
BstSCI CCNGG 1 cut(s) 525
BstSFI CTRYAG 1 cut(s) 22
BstSLI GKGCMC 1 cut(s) 268
BstV1I GCAGC 4 cut(s) 28, 104, 292, 335
BstV2I GAAGAC 1 cut(s) 61
BstX2I RGATCY 1 cut(s) 295
BstYI RGATCY 1 cut(s) 295
BtgI CCRYGG 2 cut(s) 144, 190
BtsCI GGATG 3 cut(s) 156, 186, 277
BtsIMutI CAGTG 1 cut(s) 266
Csp6I GTAC 3 cut(s) 154, 225, 433
CviAII CATG 5 cut(s) 5, 145, 458, 489, 592
CviQI GTAC 3 cut(s) 154, 225, 433
DdeI CTNAG 1 cut(s) 288
DpnI GATC 2 cut(s) 297, 393
DpnII GATC 2 cut(s) 295, 391
DrdI GACNNNNNNGTC 1 cut(s) 410
DseDI GACNNNNNNGTC 1 cut(s) 410
Eam1104I CTCTTC 1 cut(s) 525
EarI CTCTTC 1 cut(s) 525
EciI GGCGGA 1 cut(s) 512
Eco130I CCWWGG 1 cut(s) 144
Eco31I GGTCTC 1 cut(s) 418
Eco32I GATATC 1 cut(s) 565
Eco57I CTGAAG 2 cut(s) 60, 528
EcoRII CCWGG 1 cut(s) 525
EcoRV GATATC 1 cut(s) 565
EcoT14I CCWWGG 1 cut(s) 144
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 144
FaeI CATG 5 cut(s) 8, 148, 461, 492, 595
FaqI GGGAC 2 cut(s) 439, 470
FatI CATG 5 cut(s) 4, 144, 457, 488, 591
Fnu4HI GCNGC 4 cut(s) 42, 118, 306, 349
FokI GGATG 3 cut(s) 143, 173, 284
Fsp4HI GCNGC 4 cut(s) 42, 118, 306, 349
FspBI CTAG 2 cut(s) 449, 600
GluI GCNGC 4 cut(s) 42, 118, 306, 349
Hin1II CATG 5 cut(s) 8, 148, 461, 492, 595
HindIII AAGCTT 1 cut(s) 611
HinfI GANTC 3 cut(s) 8, 452, 533
HphI GGTGA 1 cut(s) 244
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 2 cut(s) 343, 582
Hpy188III TCNNGA 5 cut(s) 34, 427, 449, 464, 506
Hpy8I GTNNAC 1 cut(s) 383
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 1 cut(s) 272
HpyF3I CTNAG 1 cut(s) 288
Hsp92II CATG 5 cut(s) 8, 148, 461, 492, 595
KpnI GGTACC 1 cut(s) 436
Kzo9I GATC 2 cut(s) 295, 391
Lsp1109I GCAGC 4 cut(s) 28, 104, 292, 335
LweI GCATC 1 cut(s) 262
MaeI CTAG 2 cut(s) 449, 600
MaeIII GTNAC 1 cut(s) 280
MalI GATC 2 cut(s) 297, 393
MboI GATC 2 cut(s) 295, 391
MboII GAAGA 3 cut(s) 61, 386, 542
MflI RGATCY 1 cut(s) 295
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 1 cut(s) 208
MlyI GAGTC 2 cut(s) 461, 542
MnlI CCTC 6 cut(s) 285, 343, 346, 362, 416, 423
Mph1103I ATGCAT 1 cut(s) 6
MroXI GAANNNNTTC 1 cut(s) 68
MseI TTAA 4 cut(s) 207, 255, 500, 570
MspA1I CMGCKG 1 cut(s) 637
MspR9I CCNGG 1 cut(s) 527
Mva1269I GAATGC 2 cut(s) 43, 119
MvaI CCWGG 1 cut(s) 527
MwoI GCNNNNNNNGC 1 cut(s) 272
NcoI CCATGG 1 cut(s) 144
NdeII GATC 2 cut(s) 295, 391
NlaIII CATG 5 cut(s) 8, 148, 461, 492, 595
NlaIV GGNNCC 2 cut(s) 150, 434
NmuCI GTSAC 1 cut(s) 280
NsiI ATGCAT 1 cut(s) 6
PctI GAATGC 2 cut(s) 43, 119
PdmI GAANNNNTTC 1 cut(s) 68
PfeI GAWTC 1 cut(s) 8
PkrI GCNGC 4 cut(s) 43, 119, 307, 350
PleI GAGTC 2 cut(s) 460, 541
PpsI GAGTC 2 cut(s) 460, 541
Psp6I CCWGG 1 cut(s) 525
PspGI CCWGG 1 cut(s) 525
PspN4I GGNNCC 2 cut(s) 150, 434
PsuI RGATCY 1 cut(s) 295
PvuII CAGCTG 1 cut(s) 637
RsaI GTAC 3 cut(s) 155, 226, 434
RsaNI GTAC 3 cut(s) 154, 225, 433
SaqAI TTAA 4 cut(s) 207, 255, 500, 570
SatI GCNGC 4 cut(s) 42, 118, 306, 349
Sau3AI GATC 2 cut(s) 295, 391
SchI GAGTC 2 cut(s) 461, 542
ScrFI CCNGG 1 cut(s) 527
SduI GDGCHC 1 cut(s) 268
SfaNI GCATC 1 cut(s) 262
SfcI CTRYAG 1 cut(s) 22
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
Sse9I AATT 1 cut(s) 208
SsiI CCGC 2 cut(s) 471, 523
SspI AATATT 1 cut(s) 238
SspMI CTAG 2 cut(s) 449, 600
StyD4I CCNGG 1 cut(s) 525
StyI CCWWGG 1 cut(s) 144
TaqI TCGA 2 cut(s) 390, 465
TasI AATT 1 cut(s) 208
TfiI GAWTC 1 cut(s) 8
Tru1I TTAA 4 cut(s) 207, 255, 500, 570
Tru9I TTAA 4 cut(s) 207, 255, 500, 570
TscAI CASTG 1 cut(s) 273
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 4 cut(s) 41, 117, 305, 348
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 1 cut(s) 175
TspGWI ACGGA 2 cut(s) 141, 179
TspRI CASTG 1 cut(s) 273
XbaI TCTAGA 1 cut(s) 448
XmnI GAANNNNTTC 1 cut(s) 68
XspI CTAG 2 cut(s) 449, 600
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.