Rmu_sc0006553.1_g000014

Domain of unknown function (DUF296)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006553.1
OG0013944
Physical Location & Seq
Forward (+)
100959 .. 104744
3786 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
atgccggcgggggccgggtccgtggcggtgaagaagaagagagggaggccgaggaagtatgggccagatgggagcgtgactatggcgctgtcaccgaagccgatatcgtcggcggcgccgcctccggtgattgatttcgcggcggagaagcagccgcgtgggaaagtgaagccggccagctcagtgagcaagaacaagtacgagatggagaatttaggtgaatgggttgcatgctctgttggtgctaattttactcctcatatcatcaatgttaattctggcgaggatgtcatgatgaagattatatcattttctcaacaaggtcctcgagctatatgtgtgctctccgcaaatggtgttatatcaagtgttacacttcgtcaacctgattcttctggtggtacattgacatacgagggtcgtttcgagatactgtctttatctggttcctttatgccaaatgaaagtggagggacaagaagcagatcaggtgggatgagtgtttctttagcaagtccagatgggcgtgtcgtaggtggtggagttgctggactattagttgctgcaagtcctgtgcaggttgtagttggcagttttctggcgggaaaccagcatgagcagaaacccaagaaacagaaacatgaatacatttctaatgcaacaccaactatggctattcctgtttcaagtgctgaaccaaagacaaacctctcatcttcgacttccttccgtggtgataattggtcttcgtatcaatccgatcataaaaataagactgacattaatgtatcattgcctggaggtgtaatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF296

Protein Analysis

270

Amino Acids

28.18

Weight (kDa)

9.67

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PCC PF03479 85 - 199 1.1e-26 Plants and Prokaryotes Conserved (PCC) domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013944)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G12080
fragaria_vesca FvH4_5g00860
malus_domestica MD00G1083100.v1.1 MD06G1063800.v1.1
prunus_persica Prupe.5G081500_v2.0.a1
pyrus_communis pycom04g06900 pycom06g06420
rosa_chinensis RchiOBHm_Chr7g0201041
rosa_laevigata RLG00000003696
rosa_multiflora Rmu_sc0006553.1_g000014
rosa_roxburghii Rroxscaffold_3G00255570
rosa_rugosa Rorug07G0061700
rosa_samantha Rh7AG187500 Rh7BG190800 Rh7CG198500 Rh7DG193100
rosa_wichuraiana Rw7G016450

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 568
AccB1I GGYRCC 1 cut(s) 115
AccII CGCG 2 cut(s) 140, 157
AciI CCGC 9 cut(s) 8, 26, 113, 119, 140, 143, 155, 348, 602
AcoI YGGCCR 1 cut(s) 174
AcsI RAATTY 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 116
AfaI GTAC 2 cut(s) 200, 403
AgsI TTSAA 1 cut(s) 687
AjnI CCWGG 1 cut(s) 796
AluBI AGCT 2 cut(s) 180, 332
AluI AGCT 2 cut(s) 180, 332
Alw21I GWGCWC 1 cut(s) 345
Ama87I CYCGRG 1 cut(s) 327
AoxI GGCC 4 cut(s) 12, 47, 62, 174
ApeKI GCWGC 2 cut(s) 151, 563
ApoI RAATTY 1 cut(s) 211
AseI ATTAAT 1 cut(s) 783
AspLEI GCGC 2 cut(s) 88, 118
AspS9I GGNCC 4 cut(s) 12, 18, 62, 323
AsuC2I CCSGG 1 cut(s) 16
AsuHPI GGTGA 5 cut(s) 40, 84, 139, 230, 746
AvaI CYCGRG 1 cut(s) 327
AvaII GGWCC 2 cut(s) 18, 323
BanI GGYRCC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 738
Bbv12I GWGCWC 1 cut(s) 345
BbvI GCAGC 2 cut(s) 163, 550
BccI CCATC 3 cut(s) 62, 199, 515
BciT130I CCWGG 1 cut(s) 798
BcnI CCSGG 1 cut(s) 16
BfoI RGCGCY 2 cut(s) 89, 119
BfuAI ACCTGC 1 cut(s) 568
BisI GCNGC 6 cut(s) 114, 119, 141, 152, 155, 564
BlsI GCNGC 6 cut(s) 115, 120, 142, 153, 156, 565
Bme1390I CCNGG 2 cut(s) 16, 798
Bme18I GGWCC 2 cut(s) 18, 323
BmeT110I CYCGRG 1 cut(s) 327
BmgT120I GGNCC 4 cut(s) 12, 18, 62, 323
BmiI GGNNCC 4 cut(s) 13, 19, 117, 448
BmrFI CCNGG 2 cut(s) 16, 798
BpiI GAAGAC 1 cut(s) 738
BpuMI CCSGG 1 cut(s) 16
BsaHI GRCGYC 1 cut(s) 116
BsaJI CCNNGG 3 cut(s) 21, 50, 730
BsaWI WCCGGW 1 cut(s) 124
Bse118I RCCGGY 2 cut(s) 4, 172
Bse3DI GCAATG 1 cut(s) 791
BseBI CCWGG 1 cut(s) 798
BseDI CCNNGG 3 cut(s) 21, 50, 730
BseGI GGATG 2 cut(s) 292, 501
BseMI GCAATG 1 cut(s) 791
BseMII CTCAG 1 cut(s) 195
BseRI GAGGAG 1 cut(s) 246
BseXI GCAGC 2 cut(s) 163, 550
BsgI GTGCAG 1 cut(s) 596
Bsh1236I CGCG 2 cut(s) 140, 157
BshFI GGCC 4 cut(s) 14, 49, 64, 176
BshNI GGYRCC 1 cut(s) 115
BsiHKAI GWGCWC 1 cut(s) 345
BsiHKCI CYCGRG 1 cut(s) 327
BsiSI CCGG 4 cut(s) 5, 15, 125, 173
BslFI GGGAC 1 cut(s) 487
BsmFI GGGAC 1 cut(s) 487
BsnI GGCC 4 cut(s) 14, 49, 64, 176
BsoBI CYCGRG 1 cut(s) 327
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 2 cut(s) 485, 760
BspACI CCGC 9 cut(s) 8, 26, 113, 119, 140, 143, 155, 348, 602
BspANI GGCC 4 cut(s) 14, 49, 64, 176
BspCNI CTCAG 1 cut(s) 194
BspFNI CGCG 2 cut(s) 140, 157
BspHI TCATGA 1 cut(s) 291
BspLI GGNNCC 4 cut(s) 13, 19, 117, 448
BspMI ACCTGC 1 cut(s) 568
BspT107I GGYRCC 1 cut(s) 115
BsrDI GCAATG 1 cut(s) 791
BsrFI RCCGGY 2 cut(s) 4, 172
BssAI RCCGGY 2 cut(s) 4, 172
BssECI CCNNGG 3 cut(s) 21, 50, 730
BssMI GATC 2 cut(s) 485, 760
BssNI GRCGYC 1 cut(s) 116
Bst2UI CCWGG 1 cut(s) 798
Bst4CI ACNGT 1 cut(s) 435
Bst6I CTCTTC 1 cut(s) 32
BstACI GRCGYC 1 cut(s) 116
BstC8I GCNNGC 4 cut(s) 6, 174, 178, 232
BstDEI CTNAG 1 cut(s) 181
BstDSI CCRYGG 2 cut(s) 21, 730
BstF5I GGATG 2 cut(s) 292, 501
BstFNI CGCG 2 cut(s) 140, 157
BstH2I RGCGCY 2 cut(s) 89, 119
BstHHI GCGC 2 cut(s) 88, 118
BstKTI GATC 2 cut(s) 488, 763
BstMBI GATC 2 cut(s) 485, 760
BstMWI GCNNNNNNNGC 1 cut(s) 186
BstNI CCWGG 1 cut(s) 798
BstNSI RCATGY 1 cut(s) 234
BstSCI CCNGG 2 cut(s) 14, 796
BstUI CGCG 2 cut(s) 140, 157
BstV1I GCAGC 2 cut(s) 163, 550
BstV2I GAAGAC 1 cut(s) 738
BsuRI GGCC 4 cut(s) 14, 49, 64, 176
BtgI CCRYGG 2 cut(s) 21, 730
BtsCI GGATG 2 cut(s) 292, 501
BtsIMutI CAGTG 1 cut(s) 189
BveI ACCTGC 1 cut(s) 568
Cac8I GCNNGC 4 cut(s) 6, 174, 178, 232
CciI TCATGA 1 cut(s) 291
CfoI GCGC 2 cut(s) 88, 118
Cfr10I RCCGGY 2 cut(s) 4, 172
Cfr13I GGNCC 4 cut(s) 12, 18, 62, 323
Csp6I GTAC 2 cut(s) 199, 402
CviAII CATG 4 cut(s) 231, 292, 614, 641
CviQI GTAC 2 cut(s) 199, 402
DdeI CTNAG 1 cut(s) 181
DinI GGCGCC 1 cut(s) 117
DpnI GATC 2 cut(s) 487, 762
DpnII GATC 2 cut(s) 485, 760
EaeI YGGCCR 1 cut(s) 174
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
EciI GGCGGA 1 cut(s) 158
Eco32I GATATC 1 cut(s) 105
Eco47I GGWCC 2 cut(s) 18, 323
Eco88I CYCGRG 1 cut(s) 327
EcoO109I RGGNCCY 1 cut(s) 323
EcoRII CCWGG 1 cut(s) 796
EcoRV GATATC 1 cut(s) 105
EgeI GGCGCC 1 cut(s) 117
EheI GGCGCC 1 cut(s) 117
FaeI CATG 4 cut(s) 234, 295, 617, 644
FaqI GGGAC 1 cut(s) 487
FatI CATG 4 cut(s) 230, 291, 613, 640
FauI CCCGC 1 cut(s) 595
Fnu4HI GCNGC 6 cut(s) 114, 119, 141, 152, 155, 564
FokI GGATG 2 cut(s) 299, 508
Fsp4HI GCNGC 6 cut(s) 114, 119, 141, 152, 155, 564
GlaI GCGC 2 cut(s) 87, 117
GluI GCNGC 6 cut(s) 114, 119, 141, 152, 155, 564
HaeII RGCGCY 2 cut(s) 89, 119
HaeIII GGCC 4 cut(s) 14, 49, 64, 176
HapII CCGG 4 cut(s) 5, 15, 125, 173
HhaI GCGC 2 cut(s) 88, 118
Hin1I GRCGYC 1 cut(s) 116
Hin1II CATG 4 cut(s) 234, 295, 617, 644
Hin6I GCGC 2 cut(s) 86, 116
HinP1I GCGC 2 cut(s) 86, 116
HincII GTYRAC 1 cut(s) 383
HindII GTYRAC 1 cut(s) 383
HinfI GANTC 1 cut(s) 389
HpaII CCGG 4 cut(s) 5, 15, 125, 173
HphI GGTGA 5 cut(s) 40, 84, 139, 230, 746
Hpy166II GTNNAC 1 cut(s) 383
Hpy188I TCNGA 1 cut(s) 760
Hpy188III TCNNGA 3 cut(s) 292, 427, 518
Hpy8I GTNNAC 1 cut(s) 383
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 1 cut(s) 736
HpyCH4III ACNGT 1 cut(s) 435
HpyCH4V TGCA 4 cut(s) 230, 566, 577, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 186
HpyF3I CTNAG 1 cut(s) 181
Hsp92I GRCGYC 1 cut(s) 116
Hsp92II CATG 4 cut(s) 234, 295, 617, 644
HspAI GCGC 2 cut(s) 86, 116
KasI GGCGCC 1 cut(s) 115
KroI GCCGGC 2 cut(s) 4, 172
KroNI GCCGGC 2 cut(s) 6, 174
Kzo9I GATC 2 cut(s) 485, 760
LmnI GCTCC 1 cut(s) 72
Lsp1109I GCAGC 2 cut(s) 163, 550
MaeIII GTNAC 3 cut(s) 76, 90, 370
MalI GATC 2 cut(s) 487, 762
MboI GATC 2 cut(s) 485, 760
MboII GAAGA 7 cut(s) 43, 46, 49, 310, 384, 708, 738
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 4 cut(s) 211, 247, 274, 739
Mly113I GGCGCC 1 cut(s) 116
MroNI GCCGGC 2 cut(s) 4, 172
MseI TTAA 2 cut(s) 273, 783
MspI CCGG 4 cut(s) 5, 15, 125, 173
MspR9I CCNGG 2 cut(s) 16, 798
MvaI CCWGG 1 cut(s) 798
MvnI CGCG 2 cut(s) 140, 157
MwoI GCNNNNNNNGC 1 cut(s) 186
NaeI GCCGGC 2 cut(s) 6, 174
NarI GGCGCC 1 cut(s) 116
NciI CCSGG 1 cut(s) 16
NdeII GATC 2 cut(s) 485, 760
NgoMIV GCCGGC 2 cut(s) 4, 172
NlaIII CATG 4 cut(s) 234, 295, 617, 644
NlaIV GGNNCC 4 cut(s) 13, 19, 117, 448
NmeAIII GCCGAG 1 cut(s) 75
NmuCI GTSAC 2 cut(s) 76, 90
NspI RCATGY 1 cut(s) 234
PaeI GCATGC 1 cut(s) 234
PaeR7I CTCGAG 1 cut(s) 327
PagI TCATGA 1 cut(s) 291
PdiI GCCGGC 2 cut(s) 6, 174
PfeI GAWTC 1 cut(s) 389
PkrI GCNGC 6 cut(s) 115, 120, 142, 153, 156, 565
PluTI GGCGCC 1 cut(s) 119
PpuMI RGGWCCY 1 cut(s) 323
PshBI ATTAAT 1 cut(s) 783
Psp5II RGGWCCY 1 cut(s) 323
Psp6I CCWGG 1 cut(s) 796
PspGI CCWGG 1 cut(s) 796
PspN4I GGNNCC 4 cut(s) 13, 19, 117, 448
PspPI GGNCC 4 cut(s) 12, 18, 62, 323
PspPPI RGGWCCY 1 cut(s) 323
PspXI VCTCGAGB 1 cut(s) 327
RsaI GTAC 2 cut(s) 200, 403
RsaNI GTAC 2 cut(s) 199, 402
SaqAI TTAA 2 cut(s) 273, 783
SatI GCNGC 6 cut(s) 114, 119, 141, 152, 155, 564
Sau3AI GATC 2 cut(s) 485, 760
Sau96I GGNCC 4 cut(s) 12, 18, 62, 323
ScrFI CCNGG 2 cut(s) 16, 798
SduI GDGCHC 1 cut(s) 345
SfoI GGCGCC 1 cut(s) 117
Sfr274I CTCGAG 1 cut(s) 327
SinI GGWCC 2 cut(s) 18, 323
SlaI CTCGAG 1 cut(s) 327
SmlI CTYRAG 1 cut(s) 327
SmoI CTYRAG 1 cut(s) 327
SphI GCATGC 1 cut(s) 234
Sse9I AATT 4 cut(s) 211, 247, 274, 739
SsiI CCGC 9 cut(s) 8, 26, 113, 119, 140, 143, 155, 348, 602
SspDI GGCGCC 1 cut(s) 115
StyD4I CCNGG 2 cut(s) 14, 796
TaaI ACNGT 1 cut(s) 435
TaqI TCGA 3 cut(s) 328, 426, 719
TasI AATT 4 cut(s) 211, 247, 274, 739
TauI GCSGC 4 cut(s) 116, 121, 143, 157
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 2 cut(s) 273, 783
Tru9I TTAA 2 cut(s) 273, 783
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 76, 90
TseI GCWGC 2 cut(s) 151, 563
Tsp45I GTSAC 2 cut(s) 76, 90
TspDTI ATGAA 3 cut(s) 311, 477, 657
TspGWI ACGGA 2 cut(s) 10, 719
TspRI CASTG 1 cut(s) 189
VpaK11BI GGWCC 2 cut(s) 18, 323
VspI ATTAAT 1 cut(s) 783
XapI RAATTY 1 cut(s) 211
XceI RCATGY 1 cut(s) 234
XhoI CTCGAG 1 cut(s) 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.