Rmu_sc0006633.1_g000004

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0006633.1
OG0015382
Physical Location & Seq
Forward (+)
12282 .. 13298
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1017 bp
atggcttccaatcttctcagaattcatacaaaaaccatcagaacaccaaacccagcaaccaccccattttcactcaccaaaaccctctcctccccctctcttcaatttcccacacaccaccactcccaactctcattcccttcaactctcctcaacaaatccaaaactccctctctatccccactactctgctccctctcctcccccaccccaccaaactcaaaagaagcagccatccaacaagccaaaacatgtctctcaaccaccttagagaagcccctcaacaacctcagactcaccagcaagctcaagaagctcaaacagccccgattccgtatcgaaatcccggtcgccgacgactcccccgagtccctatcccaactcgccctccaattcttccgagacctccccatcaaaagaaaaggctccccagtcaacaccgtaatcctatggcccgatatcacctccacagaatcggcacttaaggccttcaagtcctccccctcaagcgcagtagagcatttagacattcccgcagtcgccaacggtaataccagaatttcaaacaacgctgacgtggcggtgtttttggcgccagaaccatcccaagtggcggttatgaaaaccgttgtggatttgttgtacccgaggccggtggttattttcaaccccaagtgggggtttgaggaagaggccgagtttggtgagctgagtggatttgtgggttcgtttgaggtcattttttcgtttatgggtttggaggtgaaagggcttttgagtaaaagaaatggggtggttttcaagtgtgtgagaaatggggttgtgagcggtgagaagtgggcggttcttgttgcagaagaacaaggggaattgaaggtggtttcgacgtttaagtcgaggccttcgattgctgaagttgagtatgtgttgtataatttgatggccatgaattcgcctgtaacgaaatccgtgaagttctttaaggatttggtgtcaaatgtaaccggaaagaagtaa
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

338

Amino Acids

37.08

Weight (kDa)

9.58

Isoelectric Point (pI)

43.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 72 - 307 9.6e-38 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G20680
fragaria_vesca FvH4_3g21740
malus_domestica MD03G1212500.v1.1 MD11G1227700.v1.1
prunus_persica Prupe.4G201600_v2.0.a1
pyrus_communis pycom03g16120
rosa_chinensis RchiOBHm_Chr5g0037211
rosa_laevigata RLG00000033765
rosa_multiflora Rmu_co8240257.1_g000001 Rmu_sc0006633.1_g000004
rosa_roxburghii Rroxscaffold_1G00043760
rosa_rugosa Rorug05G0163800
rosa_samantha Rh5BG255400 Rh5CG288100 Rh5DG263700
rosa_wichuraiana Rw5G023400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 892
AccB1I GGYRCC 1 cut(s) 592
AccBSI CCGCTC 1 cut(s) 828
AciI CCGC 5 cut(s) 534, 581, 614, 828, 842
AcoI YGGCCR 1 cut(s) 942
AcsI RAATTY 3 cut(s) 21, 558, 949
AcuI CTGAAG 1 cut(s) 933
AcyI GRCGYC 1 cut(s) 593
AfaI GTAC 1 cut(s) 644
AfiI CCNNNNNNNGG 5 cut(s) 613, 652, 676, 677, 678
AflII CTTAAG 1 cut(s) 482
AflIII ACRYGT 1 cut(s) 251
AgsI TTSAA 7 cut(s) 104, 144, 493, 564, 667, 802, 874
AjiI CACGTC 1 cut(s) 577
AluBI AGCT 3 cut(s) 307, 316, 709
AluI AGCT 3 cut(s) 307, 316, 709
Alw26I GTCTC 2 cut(s) 260, 396
Ama87I CYCGRG 2 cut(s) 365, 646
AoxI GGCC 6 cut(s) 452, 486, 650, 693, 899, 942
ApeKI GCWGC 1 cut(s) 230
ApoI RAATTY 3 cut(s) 21, 558, 949
ArsI GACNNNNNNTTYG 2 cut(s) 333, 365
AspLEI GCGC 2 cut(s) 512, 595
AspS9I GGNCC 1 cut(s) 453
AsuC2I CCSGG 1 cut(s) 347
AsuHPI GGTGA 6 cut(s) 67, 289, 454, 716, 775, 842
AvaI CYCGRG 2 cut(s) 365, 646
BalI TGGCCA 1 cut(s) 944
BanI GGYRCC 1 cut(s) 592
BbvI GCAGC 1 cut(s) 242
BccI CCATC 5 cut(s) 44, 242, 419, 610, 934
BcnI CCSGG 1 cut(s) 347
BcoDI GTCTC 2 cut(s) 260, 396
BfoI RGCGCY 1 cut(s) 596
BfrI CTTAAG 1 cut(s) 482
BisI GCNGC 1 cut(s) 231
BlsI GCNGC 1 cut(s) 232
Bme1390I CCNGG 1 cut(s) 347
BmeT110I CYCGRG 2 cut(s) 365, 646
BmgBI CACGTC 1 cut(s) 577
BmgT120I GGNCC 1 cut(s) 453
BmiI GGNNCC 2 cut(s) 427, 594
BmrFI CCNGG 1 cut(s) 347
BmrI ACTGGG 1 cut(s) 425
BmuI ACTGGG 1 cut(s) 425
BpuEI CTTGAG 2 cut(s) 293, 490
BpuMI CCSGG 1 cut(s) 347
BsaHI GRCGYC 1 cut(s) 593
BsaI GGTCTC 1 cut(s) 396
BsaJI CCNNGG 1 cut(s) 647
BsaWI WCCGGW 1 cut(s) 1004
BsaXI ACNNNNNCTCC 4 cut(s) 107, 137, 372, 402
Bsc4I CCNNNNNNNGG 5 cut(s) 613, 652, 676, 677, 678
Bse118I RCCGGY 1 cut(s) 652
Bse1I ACTGG 1 cut(s) 431
BseDI CCNNGG 1 cut(s) 647
BseGI GGATG 2 cut(s) 234, 602
BseLI CCNNNNNNNGG 5 cut(s) 613, 652, 676, 677, 678
BseMII CTCAG 3 cut(s) 31, 304, 701
BseNI ACTGG 1 cut(s) 431
BseRI GAGGAG 3 cut(s) 79, 140, 190
BseXI GCAGC 1 cut(s) 242
BseYI CCCAGC 1 cut(s) 52
Bsh1285I CGRYCG 1 cut(s) 351
BshFI GGCC 6 cut(s) 454, 488, 652, 695, 901, 944
BshNI GGYRCC 1 cut(s) 592
BsiEI CGRYCG 1 cut(s) 351
BsiHKCI CYCGRG 2 cut(s) 365, 646
BsiSI CCGG 3 cut(s) 347, 653, 1005
BslFI GGGAC 1 cut(s) 355
BslI CCNNNNNNNGG 5 cut(s) 613, 652, 676, 677, 678
BsmAI GTCTC 2 cut(s) 260, 396
BsmFI GGGAC 1 cut(s) 355
BsnI GGCC 6 cut(s) 454, 488, 652, 695, 901, 944
Bso31I GGTCTC 1 cut(s) 396
BsoBI CYCGRG 2 cut(s) 365, 646
BspACI CCGC 5 cut(s) 534, 581, 614, 828, 842
BspANI GGCC 6 cut(s) 454, 488, 652, 695, 901, 944
BspCNI CTCAG 3 cut(s) 30, 303, 702
BspLI GGNNCC 2 cut(s) 427, 594
BspT107I GGYRCC 1 cut(s) 592
BspTI CTTAAG 1 cut(s) 482
BspTNI GGTCTC 1 cut(s) 396
BsrBI CCGCTC 1 cut(s) 828
BsrFI RCCGGY 1 cut(s) 652
BsrI ACTGG 1 cut(s) 431
BssAI RCCGGY 1 cut(s) 652
BssECI CCNNGG 1 cut(s) 647
BssNI GRCGYC 1 cut(s) 593
Bst4CI ACNGT 3 cut(s) 442, 548, 628
Bst6I CTCTTC 2 cut(s) 105, 684
BstACI GRCGYC 1 cut(s) 593
BstAFI CTTAAG 1 cut(s) 482
BstC8I GCNNGC 1 cut(s) 305
BstDEI CTNAG 4 cut(s) 17, 268, 290, 710
BstF5I GGATG 2 cut(s) 234, 602
BstH2I RGCGCY 1 cut(s) 596
BstHHI GCGC 2 cut(s) 512, 595
BstMAI GTCTC 2 cut(s) 260, 396
BstMCI CGRYCG 1 cut(s) 351
BstMWI GCNNNNNNNGC 4 cut(s) 313, 322, 485, 578
BstNSI RCATGY 1 cut(s) 255
BstSCI CCNGG 1 cut(s) 345
BstV1I GCAGC 1 cut(s) 242
BsuRI GGCC 6 cut(s) 454, 488, 652, 695, 901, 944
BtrI CACGTC 1 cut(s) 577
BtsCI GGATG 2 cut(s) 234, 602
Cac8I GCNNGC 1 cut(s) 305
CfoI GCGC 2 cut(s) 512, 595
Cfr10I RCCGGY 1 cut(s) 652
Cfr13I GGNCC 1 cut(s) 453
Csp6I GTAC 1 cut(s) 643
CviAII CATG 2 cut(s) 252, 946
CviQI GTAC 1 cut(s) 643
DdeI CTNAG 4 cut(s) 17, 268, 290, 710
DinI GGCGCC 1 cut(s) 594
DrdI GACNNNNNNGTC 1 cut(s) 892
DseDI GACNNNNNNGTC 1 cut(s) 892
EaeI YGGCCR 1 cut(s) 942
Eam1104I CTCTTC 2 cut(s) 105, 684
EarI CTCTTC 2 cut(s) 105, 684
Eco147I AGGCCT 2 cut(s) 488, 901
Eco31I GGTCTC 1 cut(s) 396
Eco32I GATATC 1 cut(s) 460
Eco57I CTGAAG 1 cut(s) 933
Eco88I CYCGRG 2 cut(s) 365, 646
EcoRI GAATTC 2 cut(s) 21, 949
EcoRV GATATC 1 cut(s) 460
EgeI GGCGCC 1 cut(s) 594
EheI GGCGCC 1 cut(s) 594
FaeI CATG 2 cut(s) 255, 949
FaiI YATR 8 cut(s) 27, 253, 451, 620, 752, 924, 933, 947
FaqI GGGAC 1 cut(s) 355
FatI CATG 2 cut(s) 251, 945
FauI CCCGC 1 cut(s) 541
Fnu4HI GCNGC 1 cut(s) 231
FokI GGATG 2 cut(s) 221, 589
Fsp4HI GCNGC 1 cut(s) 231
GlaI GCGC 2 cut(s) 511, 594
GluI GCNGC 1 cut(s) 231
GsaI CCCAGC 1 cut(s) 56
HaeII RGCGCY 1 cut(s) 596
HaeIII GGCC 6 cut(s) 454, 488, 652, 695, 901, 944
HapII CCGG 3 cut(s) 347, 653, 1005
HhaI GCGC 2 cut(s) 512, 595
Hin1I GRCGYC 1 cut(s) 593
Hin1II CATG 2 cut(s) 255, 949
Hin6I GCGC 2 cut(s) 510, 593
HinP1I GCGC 2 cut(s) 510, 593
HincII GTYRAC 1 cut(s) 436
HindII GTYRAC 1 cut(s) 436
HinfI GANTC 5 cut(s) 294, 330, 359, 368, 473
HpaII CCGG 3 cut(s) 347, 653, 1005
HphI GGTGA 6 cut(s) 67, 289, 454, 716, 775, 842
Hpy166II GTNNAC 1 cut(s) 436
Hpy188I TCNGA 4 cut(s) 20, 41, 293, 401
Hpy188III TCNNGA 1 cut(s) 310
Hpy8I GTNNAC 1 cut(s) 436
Hpy99I CGWCG 2 cut(s) 359, 889
HpyAV CCTTC 4 cut(s) 150, 499, 868, 912
HpyCH4III ACNGT 3 cut(s) 442, 548, 628
HpyCH4IV ACGT 2 cut(s) 576, 887
HpyCH4V TGCA 1 cut(s) 854
HpyF10VI GCNNNNNNNGC 4 cut(s) 313, 322, 485, 578
HpyF3I CTNAG 4 cut(s) 17, 268, 290, 710
HpySE526I ACGT 2 cut(s) 576, 887
Hsp92I GRCGYC 1 cut(s) 593
Hsp92II CATG 2 cut(s) 255, 949
HspAI GCGC 2 cut(s) 510, 593
KasI GGCGCC 1 cut(s) 592
LmnI GCTCC 2 cut(s) 197, 431
LpnPI CCDG 8 cut(s) 66, 313, 360, 444, 568, 609, 666, 969
Lsp1109I GCAGC 1 cut(s) 242
MaeII ACGT 2 cut(s) 576, 887
MaeIII GTNAC 2 cut(s) 958, 1000
MbiI CCGCTC 1 cut(s) 828
MboII GAAGA 5 cut(s) 5, 92, 388, 701, 869
MlsI TGGCCA 1 cut(s) 944
MluCI AATT 7 cut(s) 21, 104, 392, 558, 869, 934, 949
MluNI TGGCCA 1 cut(s) 944
Mly113I GGCGCC 1 cut(s) 593
MlyI GAGTC 3 cut(s) 288, 353, 377
MmeI TCCRAC 1 cut(s) 262
Mox20I TGGCCA 1 cut(s) 944
MscI TGGCCA 1 cut(s) 944
MseI TTAA 3 cut(s) 483, 891, 981
Msp20I TGGCCA 1 cut(s) 944
MspCI CTTAAG 1 cut(s) 482
MspI CCGG 3 cut(s) 347, 653, 1005
MspR9I CCNGG 1 cut(s) 347
MwoI GCNNNNNNNGC 4 cut(s) 313, 322, 485, 578
NarI GGCGCC 1 cut(s) 593
NciI CCSGG 1 cut(s) 347
NlaIII CATG 2 cut(s) 255, 949
NlaIV GGNNCC 2 cut(s) 427, 594
NmeAIII GCCGAG 1 cut(s) 721
NspI RCATGY 1 cut(s) 255
PceI AGGCCT 2 cut(s) 488, 901
PciI ACATGT 1 cut(s) 251
PcsI WCGNNNNNNNCGW 4 cut(s) 363, 893, 902, 959
PfeI GAWTC 2 cut(s) 330, 473
PkrI GCNGC 1 cut(s) 232
PleI GAGTC 3 cut(s) 288, 353, 376
PluTI GGCGCC 1 cut(s) 596
PpsI GAGTC 3 cut(s) 288, 353, 376
PscI ACATGT 1 cut(s) 251
PspFI CCCAGC 1 cut(s) 52
PspN4I GGNNCC 2 cut(s) 427, 594
PspPI GGNCC 1 cut(s) 453
RsaI GTAC 1 cut(s) 644
RsaNI GTAC 1 cut(s) 643
SaqAI TTAA 3 cut(s) 483, 891, 981
SatI GCNGC 1 cut(s) 231
Sau96I GGNCC 1 cut(s) 453
SchI GAGTC 3 cut(s) 288, 353, 377
ScrFI CCNGG 1 cut(s) 347
SfoI GGCGCC 1 cut(s) 594
SmlI CTYRAG 3 cut(s) 308, 482, 505
SmoI CTYRAG 3 cut(s) 308, 482, 505
Sse9I AATT 7 cut(s) 21, 104, 392, 558, 869, 934, 949
SseBI AGGCCT 2 cut(s) 488, 901
SsiI CCGC 5 cut(s) 534, 581, 614, 828, 842
SspDI GGCGCC 1 cut(s) 592
StuI AGGCCT 2 cut(s) 488, 901
StyD4I CCNGG 1 cut(s) 345
TaaI ACNGT 3 cut(s) 442, 548, 628
TaiI ACGT 2 cut(s) 579, 890
TaqI TCGA 4 cut(s) 339, 884, 896, 905
TasI AATT 7 cut(s) 21, 104, 392, 558, 869, 934, 949
TfiI GAWTC 2 cut(s) 330, 473
Tru1I TTAA 3 cut(s) 483, 891, 981
Tru9I TTAA 3 cut(s) 483, 891, 981
TseI GCWGC 1 cut(s) 230
TspDTI ATGAA 3 cut(s) 14, 635, 962
TspGWI ACGGA 2 cut(s) 323, 958
Vha464I CTTAAG 1 cut(s) 482
XapI RAATTY 3 cut(s) 21, 558, 949
XceI RCATGY 1 cut(s) 255
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.