Rmu_sc0007357.1_g000003

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007357.1
OG0008347
Physical Location & Seq
Reverse (-)
15331 .. 15900
570 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 570 bp
atgatttctcaaaattgttcgccagatatcgtaacctataacacactgctgggagctctttgcagggagggaatgattgatcaggccatacagttacttgaacttttaagtggtaccagttgttcaccgggtctgatcacttacaatactgtgattgatggattggctaaatcggggtctatggaaaaagccatgggattgtatggccaaatggtagaaaatggtatcattcctgatgaaattacccatcgttctttggttggtggcttttgccgggcaaatctagttgaggaagctgctgctatactgaaggagatgcataaacgaggccaccaggtccgaactagctctttcaagtatgtgatccatggattatgtagaaacaataaggtggatattgcaatacaagttctagaattgatgatatcaaatcggtgcatgccagatgagggaatttattcaactatactcaaaggtgtaactgctgctggtatgacaaaagaggctgatcagttgcgtcagaagctgattgaatggaatgttgtacgagaagagaccatgttaatctag

Protein Analysis

189

Amino Acids

20.82

Weight (kDa)

5.82

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 3 - 53 4.5e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 5 - 34 4.5e-12 PPR repeat
PPR_2 PF13041 8 - 56 7.8e-14 PPR repeat family
TPR_24 PF23276 21 - 137 1.2e-11 Fungal tetratrico peptide repeats
PPR_1 PF12854 41 - 72 9.4e-08 PPR repeat
PPR_2 PF13041 43 - 92 4.2e-15 PPR repeat family
PPR PF01535 46 - 76 2e-09 PPR repeat
PPR_long PF17177 59 - 172 6.7e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 74 - 106 9.2e-09 PPR repeat
PPR_2 PF13041 117 - 159 4.4e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 113
AccB1I GGYRCC 1 cut(s) 113
AclWI GGATC 1 cut(s) 358
AcoI YGGCCR 1 cut(s) 205
AcsI RAATTY 1 cut(s) 453
AcuI CTGAAG 1 cut(s) 329
AfaI GTAC 2 cut(s) 115, 546
AfiI CCNNNNNNNGG 1 cut(s) 449
AgsI TTSAA 4 cut(s) 101, 355, 462, 533
AjnI CCWGG 1 cut(s) 333
AluBI AGCT 4 cut(s) 56, 296, 348, 526
AluI AGCT 4 cut(s) 56, 296, 348, 526
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 548
AlwI GGATC 1 cut(s) 358
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 3 cut(s) 84, 205, 328
ApeKI GCWGC 3 cut(s) 296, 299, 485
ApoI RAATTY 1 cut(s) 453
Asp700I GAANNNNTTC 1 cut(s) 457
Asp718I GGTACC 1 cut(s) 113
AspS9I GGNCC 1 cut(s) 337
AsuC2I CCSGG 2 cut(s) 129, 275
AsuHPI GGTGA 1 cut(s) 117
AvaII GGWCC 1 cut(s) 337
BalI TGGCCA 1 cut(s) 207
BanI GGYRCC 1 cut(s) 113
BanII GRGCYC 1 cut(s) 58
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 3 cut(s) 283, 286, 472
BccI CCATC 2 cut(s) 152, 255
BciT130I CCWGG 1 cut(s) 335
BclI TGATCA 3 cut(s) 79, 135, 508
BcnI CCSGG 2 cut(s) 129, 275
BcoDI GTCTC 1 cut(s) 548
BfaI CTAG 4 cut(s) 284, 345, 413, 568
BisI GCNGC 3 cut(s) 297, 300, 486
BlsI GCNGC 3 cut(s) 298, 301, 487
Bme1390I CCNGG 3 cut(s) 129, 275, 335
Bme18I GGWCC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 337
BmiI GGNNCC 1 cut(s) 115
BmrFI CCNGG 3 cut(s) 129, 275, 335
BmsI GCATC 1 cut(s) 306
BpuMI CCSGG 2 cut(s) 129, 275
BsaI GGTCTC 1 cut(s) 548
BsaJI CCNNGG 2 cut(s) 192, 367
Bsc4I CCNNNNNNNGG 1 cut(s) 449
Bse1I ACTGG 1 cut(s) 117
BseBI CCWGG 1 cut(s) 335
BseDI CCNNGG 2 cut(s) 192, 367
BseLI CCNNNNNNNGG 1 cut(s) 449
BseNI ACTGG 1 cut(s) 117
BseXI GCAGC 3 cut(s) 283, 286, 472
BseYI CCCAGC 1 cut(s) 49
BshFI GGCC 3 cut(s) 86, 207, 330
BshNI GGYRCC 1 cut(s) 113
BsiHKAI GWGCWC 1 cut(s) 58
BsiSI CCGG 2 cut(s) 128, 274
BslI CCNNNNNNNGG 1 cut(s) 449
BsmAI GTCTC 1 cut(s) 548
BsnI GGCC 3 cut(s) 86, 207, 330
Bso31I GGTCTC 1 cut(s) 548
Bsp1286I GDGCHC 1 cut(s) 58
Bsp143I GATC 4 cut(s) 79, 135, 363, 508
Bsp19I CCATGG 2 cut(s) 192, 367
BspANI GGCC 3 cut(s) 86, 207, 330
BspLI GGNNCC 1 cut(s) 115
BspPI GGATC 1 cut(s) 358
BspT107I GGYRCC 1 cut(s) 113
BspTNI GGTCTC 1 cut(s) 548
BsrI ACTGG 1 cut(s) 117
BssECI CCNNGG 2 cut(s) 192, 367
BssMI GATC 4 cut(s) 79, 135, 363, 508
BssT1I CCWWGG 2 cut(s) 192, 367
Bst2UI CCWGG 1 cut(s) 335
Bst4CI ACNGT 2 cut(s) 93, 151
Bst6I CTCTTC 1 cut(s) 546
BstC8I GCNNGC 1 cut(s) 440
BstDSI CCRYGG 2 cut(s) 192, 367
BstKTI GATC 4 cut(s) 82, 138, 366, 511
BstMAI GTCTC 1 cut(s) 548
BstMBI GATC 4 cut(s) 79, 135, 363, 508
BstMWI GCNNNNNNNGC 1 cut(s) 523
BstNI CCWGG 1 cut(s) 335
BstNSI RCATGY 1 cut(s) 442
BstSCI CCNGG 3 cut(s) 127, 273, 333
BstV1I GCAGC 3 cut(s) 283, 286, 472
BsuRI GGCC 3 cut(s) 86, 207, 330
BtgI CCRYGG 2 cut(s) 192, 367
BtsI GCAGTG 1 cut(s) 44
BtsIMutI CAGTG 1 cut(s) 44
Cac8I GCNNGC 1 cut(s) 440
CaiI CAGNNNCTG 1 cut(s) 526
Cfr13I GGNCC 1 cut(s) 337
CseI GACGC 1 cut(s) 506
CsiI ACCWGGT 1 cut(s) 333
Csp6I GTAC 2 cut(s) 114, 545
CviAII CATG 4 cut(s) 193, 368, 439, 559
CviQI GTAC 2 cut(s) 114, 545
DpnI GATC 4 cut(s) 81, 137, 365, 510
DpnII GATC 4 cut(s) 79, 135, 363, 508
EaeI YGGCCR 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Ecl136II GAGCTC 1 cut(s) 56
Eco130I CCWWGG 2 cut(s) 192, 367
Eco24I GRGCYC 1 cut(s) 58
Eco31I GGTCTC 1 cut(s) 548
Eco32I GATATC 2 cut(s) 28, 426
Eco47I GGWCC 1 cut(s) 337
Eco53kI GAGCTC 1 cut(s) 56
Eco57I CTGAAG 1 cut(s) 329
EcoICRI GAGCTC 1 cut(s) 56
EcoRII CCWGG 1 cut(s) 333
EcoRV GATATC 2 cut(s) 28, 426
EcoT14I CCWWGG 2 cut(s) 192, 367
EcoT22I ATGCAT 1 cut(s) 321
EcoT38I GRGCYC 1 cut(s) 58
ErhI CCWWGG 2 cut(s) 192, 367
FaeI CATG 4 cut(s) 196, 371, 442, 562
FatI CATG 4 cut(s) 192, 367, 438, 558
FbaI TGATCA 3 cut(s) 79, 135, 508
Fnu4HI GCNGC 3 cut(s) 297, 300, 486
FriOI GRGCYC 1 cut(s) 58
Fsp4HI GCNGC 3 cut(s) 297, 300, 486
FspBI CTAG 4 cut(s) 284, 345, 413, 568
GluI GCNGC 3 cut(s) 297, 300, 486
GsaI CCCAGC 1 cut(s) 53
HaeIII GGCC 3 cut(s) 86, 207, 330
HapII CCGG 2 cut(s) 128, 274
HgaI GACGC 1 cut(s) 506
Hin1II CATG 4 cut(s) 196, 371, 442, 562
HpaII CCGG 2 cut(s) 128, 274
HphI GGTGA 1 cut(s) 117
Hpy166II GTNNAC 1 cut(s) 125
Hpy188I TCNGA 3 cut(s) 135, 341, 522
Hpy188III TCNNGA 2 cut(s) 233, 413
Hpy8I GTNNAC 1 cut(s) 125
HpyAV CCTTC 1 cut(s) 304
HpyCH4III ACNGT 2 cut(s) 93, 151
HpyCH4V TGCA 4 cut(s) 63, 319, 401, 438
HpyF10VI GCNNNNNNNGC 1 cut(s) 523
Hsp92II CATG 4 cut(s) 196, 371, 442, 562
KpnI GGTACC 1 cut(s) 117
Ksp22I TGATCA 3 cut(s) 79, 135, 508
Kzo9I GATC 4 cut(s) 79, 135, 363, 508
LmnI GCTCC 1 cut(s) 53
Lsp1109I GCAGC 3 cut(s) 283, 286, 472
LweI GCATC 1 cut(s) 306
MabI ACCWGGT 1 cut(s) 333
MaeI CTAG 4 cut(s) 284, 345, 413, 568
MaeIII GTNAC 3 cut(s) 31, 93, 478
MalI GATC 4 cut(s) 81, 137, 365, 510
MboI GATC 4 cut(s) 79, 135, 363, 508
MboII GAAGA 1 cut(s) 563
MhlI GDGCHC 1 cut(s) 58
MlsI TGGCCA 1 cut(s) 207
MluCI AATT 4 cut(s) 13, 240, 416, 453
MluNI TGGCCA 1 cut(s) 207
MnlI CCTC 5 cut(s) 61, 283, 320, 442, 496
Mox20I TGGCCA 1 cut(s) 207
Mph1103I ATGCAT 1 cut(s) 321
MroXI GAANNNNTTC 1 cut(s) 457
MscI TGGCCA 1 cut(s) 207
MseI TTAA 2 cut(s) 107, 563
Msp20I TGGCCA 1 cut(s) 207
MspI CCGG 2 cut(s) 128, 274
MspR9I CCNGG 3 cut(s) 129, 275, 335
MvaI CCWGG 1 cut(s) 335
MwoI GCNNNNNNNGC 1 cut(s) 523
NciI CCSGG 2 cut(s) 129, 275
NcoI CCATGG 2 cut(s) 192, 367
NdeII GATC 4 cut(s) 79, 135, 363, 508
NlaIII CATG 4 cut(s) 196, 371, 442, 562
NlaIV GGNNCC 1 cut(s) 115
NsiI ATGCAT 1 cut(s) 321
NspI RCATGY 1 cut(s) 442
PaeI GCATGC 1 cut(s) 442
PdmI GAANNNNTTC 1 cut(s) 457
PkrI GCNGC 3 cut(s) 298, 301, 487
Psp124BI GAGCTC 1 cut(s) 58
Psp6I CCWGG 1 cut(s) 333
PspFI CCCAGC 1 cut(s) 49
PspGI CCWGG 1 cut(s) 333
PspN4I GGNNCC 1 cut(s) 115
PspPI GGNCC 1 cut(s) 337
PsrI GAACNNNNNNTAC 2 cut(s) 106, 138
PstNI CAGNNNCTG 1 cut(s) 526
RsaI GTAC 2 cut(s) 115, 546
RsaNI GTAC 2 cut(s) 114, 545
SacI GAGCTC 1 cut(s) 58
SaqAI TTAA 2 cut(s) 107, 563
SatI GCNGC 3 cut(s) 297, 300, 486
Sau3AI GATC 4 cut(s) 79, 135, 363, 508
Sau96I GGNCC 1 cut(s) 337
ScrFI CCNGG 3 cut(s) 129, 275, 335
SduI GDGCHC 1 cut(s) 58
SetI ASST 8 cut(s) 38, 58, 298, 339, 350, 393, 478, 528
SexAI ACCWGGT 1 cut(s) 333
SfaNI GCATC 1 cut(s) 306
SinI GGWCC 1 cut(s) 337
SphI GCATGC 1 cut(s) 442
Sse9I AATT 4 cut(s) 13, 240, 416, 453
SspMI CTAG 4 cut(s) 284, 345, 413, 568
SstI GAGCTC 1 cut(s) 58
StyD4I CCNGG 3 cut(s) 127, 273, 333
StyI CCWWGG 2 cut(s) 192, 367
TaaI ACNGT 2 cut(s) 93, 151
TasI AATT 4 cut(s) 13, 240, 416, 453
Tru1I TTAA 2 cut(s) 107, 563
Tru9I TTAA 2 cut(s) 107, 563
TscAI CASTG 1 cut(s) 51
TseI GCWGC 3 cut(s) 296, 299, 485
TspDTI ATGAA 1 cut(s) 252
TspRI CASTG 1 cut(s) 51
VpaK11BI GGWCC 1 cut(s) 337
XapI RAATTY 1 cut(s) 453
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 1 cut(s) 442
XmnI GAANNNNTTC 1 cut(s) 457
XspI CTAG 4 cut(s) 284, 345, 413, 568
Zsp2I ATGCAT 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.