Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000006

Orthogroup ID

OG0008347

Physical Location & Seq

Genomic Coordinates Forward (+)

48110459 .. 48113275

Gene Structure 2817 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_6G00427540.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1755 bp

ATGGCGCATACCATTGCTCAACAGAATAGTACAATTGATTTCCGATGTTTGCATGTGTGTGCAAAAGAAGTTAGCCCCTGTTCTGGCATTGGTCATTTACCAATATCAAGGTTTTTAAGCAAGTATAGTCGTGAATCTCAGTGTTGTTTACGATGGGGAGGTGTTGGTTCCAAAAGCGATGTGCAGTATTTACAGCATAGTGGTTTTTGGAGAAGTGTTTGTATTGATAGAGTCCATGAAATGGACCTGGATGAATGGAGCACTGATACTGATAAGATGGGTATTGAGAAAAAATCTGGAGAAGAATTGTATGAGAAGAGGCCTCATAGGTCTTCAACAGTGGATTTAGATGGCCGTTTTGTTGGAAATGGTGAGGATAAAAACAATGATGTTCTTCGGAATTTCTGCAGCCGTGGGAAGTTACTTCTTGCATCACGACTGATTGATATCATGGCACGTCGAAACCAAATTCCGGATTTCCCTTGTTGCAAAAACTTAATCCGCGGGCTCGTTAAAATAGATCAAGTAGATAAAGCTGTGAAGATTCTAAAGATCATGGTCATGTCTGGTGGGGTTCCAGATATTATCACATATAACATGATGGTGAGTGCTCTCTGTAAGAAAGGACAGTTAAGATCTGCAATTGATCTTGTGGAAGACATGAGCTTGAGCGGTTGCCCTCCAGATGTGATTACTTACAATACAATAATCCGCGGCATGTTTGCTATTGGGAATTTTGAGCAGGCTATTGCATTTTGGAAGGAGGAACTGAGAAAGGGGTGCCCTCCTTATATAGTTACCTACACTGTTCTCATCGACCTAATCTGCAGACACTGTGGAATCATGCGGGCTGTGGAAGTTTTGGATGATATGGTAGTAGAGGGATGTTATCCTGATATTATTACATACAACTCTCTGGTTAATTTTACTAGTAGGCGGAATAAACTTGAAGATACTACTCTGGTTATTCATAATCTTCTCTCTCTTGGGTTCGATCCCAATGTTGTTACTTACAACACTATTCTCCACTCGCTTTGTGGCCGTGGGTGTTGGGATGAAGCAGATGAAATCTTCACAATCATGAATGAGACTTCTGTATCCCCTACAGTAGTTACTTACAATATTATGATCAACGGTTTGTGTAAATTTGGGATTTTAGATCGCGCGATCAACTTCTTTACTCAAATGATTTCTCAAAATTGTTCACCGGATATCATAACCTATAACACACTGCTGGGAGCTCTTTGTAGGGAGGGAATGATTGATCAGGCCATCCAGTTACTCGAACTTTTAAGTGGTACCAGTTGTTCGCCGGGTCTGATCACTTACAATACTGTGATTGATGGATTGGCTAAATCGGGGTCTATGGAGAAAGCCATGGGATTGTATGGTCAAATGGTAGAAAATGGTATCATTCCTGATGAAATTACCCATCGTTCTTTGGTTGGTGGGTTTTGCCGGGCAAATCTAGTTGAGGAAGCTGCTGCTATACTGAAGCAGATGCATAAAAGAGGCCATCAGGTCCGAACTAGCTCTTTCAAGTATGTGATCCATGGATTATGTAGAAACAATAAAGTGGATATTGCAATACAAGTTCTAGAAATGATGATATCAAATCGGTGCATGCCAGATGAGGGAATTTATTCAACTATACTCAAAGGTGTAACTGCTGCTGGTATGACGAAGGAGGCTGATCAATTGCGTCAGAAGCTGATTGCATGGAATGTTGTACGAGAAGAGACCATGTTAAACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0006139 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009451 GO:0009987 GO:0016070 GO:0016787 GO:0016788 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044464 GO:0046483 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3

Protein Analysis

584

Amino Acids

65.35

Weight (kDa)

6.38

Isoelectric Point (pI)

32.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	131 - 255	3.3e-08	Fungal tetratrico peptide repeats
PPR_2	PF13041	163 - 207	3.5e-09	PPR repeat family
PPR_1	PF12854	190 - 222	3.2e-10	PPR repeat
PPR_2	PF13041	193 - 240	7.6e-17	PPR repeat family
PPR	PF01535	196 - 226	2.1e-06	PPR repeat
PPR_3	PF13812	217 - 275	6.3e-07	Pentatricopeptide repeat domain
PPR_1	PF12854	225 - 255	4.9e-07	PPR repeat
PPR_2	PF13041	228 - 277	2.1e-12	PPR repeat family
PPR_3	PF13812	258 - 309	5.4e-06	Pentatricopeptide repeat domain
PPR_2	PF13041	264 - 308	2.5e-10	PPR repeat family
PPR_3	PF13812	322 - 378	2.2e-10	Pentatricopeptide repeat domain
PPR_1	PF12854	330 - 361	9.2e-07	PPR repeat
PPR_2	PF13041	333 - 382	4.8e-18	PPR repeat family
TPR_24	PF23276	355 - 465	1e-06	Fungal tetratrico peptide repeats
PPR_1	PF12854	366 - 396	1.3e-11	PPR repeat
PPR_2	PF13041	368 - 417	3.1e-17	PPR repeat family
PPR	PF01535	371 - 401	1.9e-06	PPR repeat
PPR_3	PF13812	394 - 448	1.3e-06	Pentatricopeptide repeat domain
PPR_1	PF12854	400 - 429	4.7e-11	PPR repeat
PPR_2	PF13041	403 - 452	4.4e-13	PPR repeat family
TPR_24	PF23276	416 - 532	2.9e-10	Fungal tetratrico peptide repeats
PPR_1	PF12854	436 - 467	4.4e-07	PPR repeat
PPR_2	PF13041	438 - 487	2.3e-14	PPR repeat family
PPR	PF01535	441 - 471	9.2e-09	PPR repeat
PPR_long	PF17177	454 - 563	1.3e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	469 - 501	9.5e-08	PPR repeat
PPR_2	PF13041	512 - 554	1.3e-06	PPR repeat family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0008347)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08610
fragaria_vesca	FvH4_6g01910 FvH4_6g01910 FvH4_6g01910 FvH4_6g01910 FvH4_6g01910
malus_domestica	MD04G1230200.v1.1
prunus_persica	Prupe.6G350100_v2.0.a1 Prupe.6G350100_v2.0.a1 Prupe.6G350100_v2.0.a1 Prupe.6G350100_v2.0.a1
pyrus_communis	pycom04g20410
rosa_chinensis	RchiOBHm_Chr3g0449641
rosa_laevigata	RLG00000025812
rosa_multiflora	Rmu_sc0007357.1_g000003 Rmu_sc0009629.1_g000014
rosa_roxburghii	Rroxscaffold_6G00427540
rosa_rugosa	Rorug02G0622600
rosa_samantha	Rh3AG020500 Rh3BG021200 Rh3CG019700 Rh3DG021000
rosa_wichuraiana	Rw3G001690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1298
AccB1I	GGYRCC	2 cut(s)	780, 1298
AccB7I	CCANNNNNTGG	1 cut(s)	241
AccBSI	CCGCTC	1 cut(s)	672
AccII	CGCG	4 cut(s)	504, 714, 1164, 1166
AccIII	TCCGGA	1 cut(s)	472
AciI	CCGC	7 cut(s)	502, 504, 672, 712, 714, 847, 937
AclWI	GGATC	2 cut(s)	989, 1543
AcoI	YGGCCR	2 cut(s)	352, 1039
AcsI	RAATTY	5 cut(s)	400, 468, 733, 1145, 1638
AcuI	CTGAAG	1 cut(s)	1514
AfaI	GTAC	3 cut(s)	31, 1300, 1731
AfiI	CCNNNNNNNGG	4 cut(s)	83, 241, 472, 1634
AgsI	TTSAA	4 cut(s)	336, 950, 1540, 1647
AhlI	ACTAGT	1 cut(s)	929
AjiI	CACGTC	1 cut(s)	458
AjnI	CCWGG	1 cut(s)	246
AluBI	AGCT	6 cut(s)	536, 666, 1241, 1481, 1533, 1711
AluI	AGCT	6 cut(s)	536, 666, 1241, 1481, 1533, 1711
Alw21I	GWGCWC	3 cut(s)	263, 613, 1243
Alw26I	GTCTC	2 cut(s)	1082, 1733
AlwI	GGATC	2 cut(s)	989, 1543
AlwNI	CAGNNNCTG	2 cut(s)	834, 1711
Aor13HI	TCCGGA	1 cut(s)	472
AoxI	GGCC	5 cut(s)	320, 352, 1039, 1269, 1513
ApeKI	GCWGC	4 cut(s)	408, 1481, 1484, 1670
ApoI	RAATTY	5 cut(s)	400, 468, 733, 1145, 1638
Asp700I	GAANNNNTTC	1 cut(s)	1642
Asp718I	GGTACC	1 cut(s)	1298
AspLEI	GCGC	2 cut(s)	7, 1166
AspS9I	GGNCC	2 cut(s)	244, 1522
AsuC2I	CCSGG	2 cut(s)	1314, 1460
AsuHPI	GGTGA	3 cut(s)	383, 616, 1197
AvaII	GGWCC	2 cut(s)	244, 1522
BaeGI	GKGCMC	1 cut(s)	785
BaeI	ACNNNNGTAYC	2 cut(s)	1080, 1113
BanI	GGYRCC	2 cut(s)	780, 1298
BanII	GRGCYC	2 cut(s)	510, 1243
BbsI	GAAGAC	2 cut(s)	324, 663
Bbv12I	GWGCWC	3 cut(s)	263, 613, 1243
BbvI	GCAGC	4 cut(s)	420, 1468, 1471, 1657
BccI	CCATC	8 cut(s)	147, 271, 344, 595, 1280, 1337, 1440, 1524
BceAI	ACGGC	3 cut(s)	339, 396, 1026
BciT130I	CCWGG	1 cut(s)	248
BciVI	GTATCC	1 cut(s)	1108
BclI	TGATCA	4 cut(s)	1128, 1264, 1320, 1693
BcnI	CCSGG	2 cut(s)	1314, 1460
BcoDI	GTCTC	2 cut(s)	1082, 1733
BcuI	ACTAGT	1 cut(s)	929
BfaI	CTAG	5 cut(s)	930, 1469, 1530, 1598, 1753
BfmI	CTRYAG	3 cut(s)	406, 826, 1104
BfuI	GTATCC	1 cut(s)	1108
BglII	AGATCT	1 cut(s)	635
BisI	GCNGC	5 cut(s)	409, 715, 1482, 1485, 1671
BlsI	GCNGC	5 cut(s)	410, 716, 1483, 1486, 1672
Bme1390I	CCNGG	3 cut(s)	248, 1314, 1460
Bme18I	GGWCC	2 cut(s)	244, 1522
BmgBI	CACGTC	1 cut(s)	458
BmgT120I	GGNCC	2 cut(s)	244, 1522
BmiI	GGNNCC	4 cut(s)	169, 576, 782, 1300
BmrFI	CCNGG	3 cut(s)	248, 1314, 1460
BmsI	GCATC	2 cut(s)	440, 1491
BpiI	GAAGAC	2 cut(s)	324, 663
BpmI	CTGGAG	2 cut(s)	318, 666
BpuEI	CTTGAG	1 cut(s)	688
BpuMI	CCSGG	2 cut(s)	1314, 1460
BsaBI	GATNNNNATC	1 cut(s)	446
BsaI	GGTCTC	1 cut(s)	1733
BsaJI	CCNNGG	6 cut(s)	412, 502, 712, 1042, 1377, 1552
BsaWI	WCCGGW	2 cut(s)	472, 1207
Bsc4I	CCNNNNNNNGG	4 cut(s)	83, 241, 472, 1634
Bse1I	ACTGG	2 cut(s)	1276, 1302
Bse3DI	GCAATG	1 cut(s)	12
Bse8I	GATNNNNATC	1 cut(s)	446
BseAI	TCCGGA	1 cut(s)	472
BseBI	CCWGG	1 cut(s)	248
BseDI	CCNNGG	6 cut(s)	412, 502, 712, 1042, 1377, 1552
BseGI	GGATG	5 cut(s)	256, 871, 890, 1060, 1272
BseJI	GATNNNNATC	1 cut(s)	446
BseLI	CCNNNNNNNGG	4 cut(s)	83, 241, 472, 1634
BseMI	GCAATG	1 cut(s)	12
BseMII	CTCAG	2 cut(s)	152, 761
BseNI	ACTGG	2 cut(s)	1276, 1302
BseSI	GKGCMC	1 cut(s)	785
BseXI	GCAGC	4 cut(s)	420, 1468, 1471, 1657
BseYI	CCCAGC	1 cut(s)	1234
BsgI	GTGCAG	1 cut(s)	203
Bsh1236I	CGCG	4 cut(s)	504, 714, 1164, 1166
BshFI	GGCC	5 cut(s)	322, 354, 1041, 1271, 1515
BshNI	GGYRCC	2 cut(s)	780, 1298
BsiHKAI	GWGCWC	3 cut(s)	263, 613, 1243
BsiSI	CCGG	4 cut(s)	473, 1208, 1313, 1459
BslI	CCNNNNNNNGG	4 cut(s)	83, 241, 472, 1634
BsmAI	GTCTC	2 cut(s)	1082, 1733
BsnI	GGCC	5 cut(s)	322, 354, 1041, 1271, 1515
Bso31I	GGTCTC	1 cut(s)	1733
Bsp1286I	GDGCHC	5 cut(s)	263, 510, 613, 785, 1243
Bsp13I	TCCGGA	1 cut(s)	472
Bsp19I	CCATGG	2 cut(s)	1377, 1552
BspACI	CCGC	7 cut(s)	502, 504, 672, 712, 714, 847, 937
BspANI	GGCC	5 cut(s)	322, 354, 1041, 1271, 1515
BspCNI	CTCAG	2 cut(s)	151, 762
BspEI	TCCGGA	1 cut(s)	472
BspFNI	CGCG	4 cut(s)	504, 714, 1164, 1166
BspHI	TCATGA	1 cut(s)	1080
BspLI	GGNNCC	4 cut(s)	169, 576, 782, 1300
BspMAI	CTGCAG	2 cut(s)	410, 830
BspPI	GGATC	2 cut(s)	989, 1543
BspT107I	GGYRCC	2 cut(s)	780, 1298
BspTNI	GGTCTC	1 cut(s)	1733
BsrBI	CCGCTC	1 cut(s)	672
BsrDI	GCAATG	1 cut(s)	12
BsrI	ACTGG	2 cut(s)	1276, 1302
BssECI	CCNNGG	6 cut(s)	412, 502, 712, 1042, 1377, 1552
BssT1I	CCWWGG	2 cut(s)	1377, 1552
Bst2UI	CCWGG	1 cut(s)	248
Bst4CI	ACNGT	7 cut(s)	340, 630, 808, 836, 1108, 1136, 1336
Bst6I	CTCTTC	2 cut(s)	311, 1731
BstC8I	GCNNGC	4 cut(s)	506, 744, 849, 1625
BstDEI	CTNAG	2 cut(s)	138, 770
BstDSI	CCRYGG	6 cut(s)	412, 502, 712, 1042, 1377, 1552
BstF5I	GGATG	5 cut(s)	256, 871, 890, 1060, 1272
BstFNI	CGCG	4 cut(s)	504, 714, 1164, 1166
BstHHI	GCGC	2 cut(s)	7, 1166
BstMAI	GTCTC	2 cut(s)	1082, 1733
BstMWI	GCNNNNNNNGC	1 cut(s)	1708
BstNI	CCWGG	1 cut(s)	248
BstNSI	RCATGY	3 cut(s)	56, 721, 1627
BstSCI	CCNGG	3 cut(s)	246, 1312, 1458
BstSFI	CTRYAG	3 cut(s)	406, 826, 1104
BstSLI	GKGCMC	1 cut(s)	785
BstUI	CGCG	4 cut(s)	504, 714, 1164, 1166
BstV1I	GCAGC	4 cut(s)	420, 1468, 1471, 1657
BstV2I	GAAGAC	2 cut(s)	324, 663
BstX2I	RGATCY	1 cut(s)	635
BstYI	RGATCY	1 cut(s)	635
BsuI	GTATCC	1 cut(s)	1108
BsuRI	GGCC	5 cut(s)	322, 354, 1041, 1271, 1515
BtgI	CCRYGG	6 cut(s)	412, 502, 712, 1042, 1377, 1552
BtgZI	GCGATG	1 cut(s)	192
BtrI	CACGTC	1 cut(s)	458
BtsCI	GGATG	5 cut(s)	256, 871, 890, 1060, 1272
BtsI	GCAGTG	1 cut(s)	1229
BtsIMutI	CAGTG	6 cut(s)	146, 261, 345, 804, 832, 1229
Cac8I	GCNNGC	4 cut(s)	506, 744, 849, 1625
CaiI	CAGNNNCTG	2 cut(s)	834, 1711
CciI	TCATGA	1 cut(s)	1080
CfoI	GCGC	2 cut(s)	7, 1166
Cfr13I	GGNCC	2 cut(s)	244, 1522
Cfr42I	CCGCGG	2 cut(s)	505, 715
CseI	GACGC	1 cut(s)	1691
Csp6I	GTAC	3 cut(s)	30, 1299, 1730
CspCI	CAANNNNNGTGG	2 cut(s)	1016, 1051
CviQI	GTAC	3 cut(s)	30, 1299, 1730
DdeI	CTNAG	2 cut(s)	138, 770
EaeI	YGGCCR	2 cut(s)	352, 1039
Eam1104I	CTCTTC	2 cut(s)	311, 1731
EarI	CTCTTC	2 cut(s)	311, 1731
EciI	GGCGGA	1 cut(s)	952
Ecl136II	GAGCTC	1 cut(s)	1241
Eco130I	CCWWGG	2 cut(s)	1377, 1552
Eco147I	AGGCCT	1 cut(s)	322
Eco24I	GRGCYC	2 cut(s)	510, 1243
Eco31I	GGTCTC	1 cut(s)	1733
Eco32I	GATATC	3 cut(s)	448, 1213, 1611
Eco47I	GGWCC	2 cut(s)	244, 1522
Eco53kI	GAGCTC	1 cut(s)	1241
Eco57I	CTGAAG	1 cut(s)	1514
EcoICRI	GAGCTC	1 cut(s)	1241
EcoRII	CCWGG	1 cut(s)	246
EcoRV	GATATC	3 cut(s)	448, 1213, 1611
EcoT14I	CCWWGG	2 cut(s)	1377, 1552
EcoT22I	ATGCAT	1 cut(s)	1506
EcoT38I	GRGCYC	2 cut(s)	510, 1243
ErhI	CCWWGG	2 cut(s)	1377, 1552
FauI	CCCGC	2 cut(s)	497, 840
FbaI	TGATCA	4 cut(s)	1128, 1264, 1320, 1693
Fnu4HI	GCNGC	5 cut(s)	409, 715, 1482, 1485, 1671
FokI	GGATG	5 cut(s)	263, 878, 897, 1067, 1259
FriOI	GRGCYC	2 cut(s)	510, 1243
Fsp4HI	GCNGC	5 cut(s)	409, 715, 1482, 1485, 1671
FspBI	CTAG	5 cut(s)	930, 1469, 1530, 1598, 1753
GlaI	GCGC	2 cut(s)	6, 1165
GluI	GCNGC	5 cut(s)	409, 715, 1482, 1485, 1671
GsaI	CCCAGC	1 cut(s)	1238
GsuI	CTGGAG	2 cut(s)	318, 666
HaeIII	GGCC	5 cut(s)	322, 354, 1041, 1271, 1515
HapII	CCGG	4 cut(s)	473, 1208, 1313, 1459
HgaI	GACGC	1 cut(s)	1691
HhaI	GCGC	2 cut(s)	7, 1166
Hin6I	GCGC	2 cut(s)	5, 1164
HinP1I	GCGC	2 cut(s)	5, 1164
HinfI	GANTC	4 cut(s)	134, 231, 544, 840
HpaII	CCGG	4 cut(s)	473, 1208, 1313, 1459
HphI	GGTGA	3 cut(s)	383, 616, 1197
Hpy166II	GTNNAC	2 cut(s)	149, 1205
Hpy188I	TCNGA	5 cut(s)	44, 399, 1320, 1526, 1707
Hpy8I	GTNNAC	2 cut(s)	149, 1205
Hpy99I	CGWCG	1 cut(s)	462
HpyAV	CCTTC	2 cut(s)	754, 1678
HpyCH4III	ACNGT	7 cut(s)	340, 630, 808, 836, 1108, 1136, 1336
HpyCH4IV	ACGT	1 cut(s)	457
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1708
HpyF3I	CTNAG	2 cut(s)	138, 770
HpySE526I	ACGT	1 cut(s)	457
HspAI	GCGC	2 cut(s)	5, 1164
Kpn2I	TCCGGA	1 cut(s)	472
KpnI	GGTACC	1 cut(s)	1302
Ksp22I	TGATCA	4 cut(s)	1128, 1264, 1320, 1693
KspI	CCGCGG	2 cut(s)	505, 715
LmnI	GCTCC	2 cut(s)	258, 1238
Lsp1109I	GCAGC	4 cut(s)	420, 1468, 1471, 1657
LweI	GCATC	2 cut(s)	440, 1491
MaeI	CTAG	5 cut(s)	930, 1469, 1530, 1598, 1753
MaeII	ACGT	1 cut(s)	457
MaeIII	GTNAC	6 cut(s)	420, 796, 1006, 1111, 1278, 1663
MbiI	CCGCTC	1 cut(s)	672
MfeI	CAATTG	3 cut(s)	33, 642, 1697
MflI	RGATCY	1 cut(s)	635
MhlI	GDGCHC	5 cut(s)	263, 510, 613, 785, 1243
MlyI	GAGTC	1 cut(s)	240
MmeI	TCCRAC	1 cut(s)	343
Mph1103I	ATGCAT	1 cut(s)	1506
MroI	TCCGGA	1 cut(s)	472
MroXI	GAANNNNTTC	1 cut(s)	1642
MseI	TTAA	7 cut(s)	116, 497, 513, 632, 921, 1292, 1748
MslI	CAYNNNNRTG	4 cut(s)	57, 560, 602, 1079
MspA1I	CMGCKG	2 cut(s)	504, 714
MspI	CCGG	4 cut(s)	473, 1208, 1313, 1459
MspR9I	CCNGG	3 cut(s)	248, 1314, 1460
MunI	CAATTG	3 cut(s)	33, 642, 1697
MvaI	CCWGG	1 cut(s)	248
MvnI	CGCG	4 cut(s)	504, 714, 1164, 1166
MwoI	GCNNNNNNNGC	1 cut(s)	1708
NciI	CCSGG	2 cut(s)	1314, 1460
NcoI	CCATGG	2 cut(s)	1377, 1552
NlaIV	GGNNCC	4 cut(s)	169, 576, 782, 1300
NsiI	ATGCAT	1 cut(s)	1506
NspI	RCATGY	3 cut(s)	56, 721, 1627
PaeI	GCATGC	1 cut(s)	1627
PagI	TCATGA	1 cut(s)	1080
PceI	AGGCCT	1 cut(s)	322
PdmI	GAANNNNTTC	1 cut(s)	1642
PfeI	GAWTC	3 cut(s)	134, 544, 840
PflMI	CCANNNNNTGG	1 cut(s)	241
PkrI	GCNGC	5 cut(s)	410, 716, 1483, 1486, 1672
PleI	GAGTC	1 cut(s)	239
PpsI	GAGTC	1 cut(s)	239
Psp124BI	GAGCTC	1 cut(s)	1243
Psp6I	CCWGG	1 cut(s)	246
PspFI	CCCAGC	1 cut(s)	1234
PspGI	CCWGG	1 cut(s)	246
PspN4I	GGNNCC	4 cut(s)	169, 576, 782, 1300
PspPI	GGNCC	2 cut(s)	244, 1522
PsrI	GAACNNNNNNTAC	2 cut(s)	1291, 1323
PstI	CTGCAG	2 cut(s)	410, 830
PstNI	CAGNNNCTG	2 cut(s)	834, 1711
PsuI	RGATCY	1 cut(s)	635
RsaI	GTAC	3 cut(s)	31, 1300, 1731
RsaNI	GTAC	3 cut(s)	30, 1299, 1730
RseI	CAYNNNNRTG	4 cut(s)	57, 560, 602, 1079
SacI	GAGCTC	1 cut(s)	1243
SacII	CCGCGG	2 cut(s)	505, 715
SaqAI	TTAA	7 cut(s)	116, 497, 513, 632, 921, 1292, 1748
SatI	GCNGC	5 cut(s)	409, 715, 1482, 1485, 1671
Sau96I	GGNCC	2 cut(s)	244, 1522
SchI	GAGTC	1 cut(s)	240
ScrFI	CCNGG	3 cut(s)	248, 1314, 1460
SduI	GDGCHC	5 cut(s)	263, 510, 613, 785, 1243
SfaNI	GCATC	2 cut(s)	440, 1491
SfcI	CTRYAG	3 cut(s)	406, 826, 1104
Sfr303I	CCGCGG	2 cut(s)	505, 715
SgrBI	CCGCGG	2 cut(s)	505, 715
SinI	GGWCC	2 cut(s)	244, 1522
SmiMI	CAYNNNNRTG	4 cut(s)	57, 560, 602, 1079
SmlI	CTYRAG	1 cut(s)	667
SmoI	CTYRAG	1 cut(s)	667
SpeI	ACTAGT	1 cut(s)	929
SphI	GCATGC	1 cut(s)	1627
SseBI	AGGCCT	1 cut(s)	322
SsiI	CCGC	7 cut(s)	502, 504, 672, 712, 714, 847, 937
SspI	AATATT	1 cut(s)	1123
SspMI	CTAG	5 cut(s)	930, 1469, 1530, 1598, 1753
SstI	GAGCTC	1 cut(s)	1243
StuI	AGGCCT	1 cut(s)	322
StyD4I	CCNGG	3 cut(s)	246, 1312, 1458
StyI	CCWWGG	2 cut(s)	1377, 1552
TaaI	ACNGT	7 cut(s)	340, 630, 808, 836, 1108, 1136, 1336
TaiI	ACGT	1 cut(s)	460
TaqI	TCGA	4 cut(s)	460, 816, 993, 1284
TatI	WGTACW	1 cut(s)	29
TauI	GCSGC	1 cut(s)	717
TfiI	GAWTC	3 cut(s)	134, 544, 840
Tru1I	TTAA	7 cut(s)	116, 497, 513, 632, 921, 1292, 1748
Tru9I	TTAA	7 cut(s)	116, 497, 513, 632, 921, 1292, 1748
TscAI	CASTG	6 cut(s)	146, 268, 345, 811, 839, 1236
TseI	GCWGC	4 cut(s)	408, 1481, 1484, 1670
TspDTI	ATGAA	7 cut(s)	252, 267, 959, 1071, 1080, 1097, 1437
TspRI	CASTG	6 cut(s)	146, 268, 345, 811, 839, 1236
Van91I	CCANNNNNTGG	1 cut(s)	241
VpaK11BI	GGWCC	2 cut(s)	244, 1522
XapI	RAATTY	5 cut(s)	400, 468, 733, 1145, 1638
XbaI	TCTAGA	1 cut(s)	1597
XceI	RCATGY	3 cut(s)	56, 721, 1627
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1034
XmnI	GAANNNNTTC	1 cut(s)	1642
XspI	CTAG	5 cut(s)	930, 1469, 1530, 1598, 1753
Zsp2I	ATGCAT	1 cut(s)	1506

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.