Rmu_sc0007538.1_g000013

cytochrome

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0007538.1
OG0012398
Physical Location & Seq
Reverse (-)
76464 .. 79097
2634 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 648 bp
atgccgacgctgacgaagctctacacaatgcaagaagcctcgcagcacaacaccaaagacgactgctggatcgtcgtcgacggcaaggtgtacgatgtgtcaacatatttggatgatcaccctggtggggatgatgtaatccttgctacaactggcaaagatgccactgacgattttgaagatgctgggcacagcaaaagtgcaaaggagctcatggaaaccatgtgcattggagagcttgacacagacacctccgcctcagctgaacttgagaatcaaccaactggttatccgcagaagctcatgaccctgacaaagcaatactgggctgttcctgcagctgttgtgggcatctctgtggtggttggcttcgtccttcaaatcccaaaaaccaccgccgcccaccttgaagatctcaactccaagagtccagaaccggcggcccccacccctgaaccggccggcaaaccaccgaaccggccaccggaagtagcaaaagtctctctgcccggttcaaagccttcctcaccacctcctgcagccatactcgaccacctacagcgtctccaccccagtaacccggaaccggaaatttcatcaccggaaccggccaaaattccatcgaacccgccaccggaatcttattaa

Protein Analysis

215

Amino Acids

23.0

Weight (kDa)

4.64

Isoelectric Point (pI)

48.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cyt-b5 PF00173 9 - 81 3.4e-29 Cytochrome b5-like Heme/Steroid binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G26340
fragaria_vesca FvH4_4g29701
malus_domestica MD13G1059800.v1.1 MD13G1059900.v1.1 MD16G1059300.v1.1
prunus_persica Prupe.1G289700_v2.0.a1
pyrus_communis pycom13g05330
rosa_chinensis RchiOBHm_Chr4g0437951
rosa_laevigata RLG00000006399
rosa_multiflora Rmu_sc0007538.1_g000013 Rmu_sc0025141.1_g000004
rosa_roxburghii Rroxscaffold_5G00379110
rosa_rugosa Rorug04G0305800
rosa_samantha Rh4AG357400 Rh4BG368300 Rh4CG382400 Rh4DG363200
rosa_wichuraiana Rw0G011530 Rw0G016140

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 78
AciI CCGC 6 cut(s) 255, 293, 396, 399, 440, 629
AclWI GGATC 1 cut(s) 77
AcoI YGGCCR 3 cut(s) 459, 479, 609
AcsI RAATTY 2 cut(s) 591, 615
AfaI GTAC 1 cut(s) 92
AfiI CCNNNNNNNGG 5 cut(s) 127, 457, 484, 586, 634
AgsI TTSAA 4 cut(s) 179, 380, 410, 516
AjnI CCWGG 1 cut(s) 121
AleI CACNNNNGTG 1 cut(s) 123
AluBI AGCT 6 cut(s) 19, 211, 238, 263, 301, 341
AluI AGCT 6 cut(s) 19, 211, 238, 263, 301, 341
Alw21I GWGCWC 1 cut(s) 213
Alw26I GTCTC 2 cut(s) 505, 569
AlwI GGATC 1 cut(s) 77
AoxI GGCC 4 cut(s) 441, 459, 479, 609
ApeKI GCWGC 3 cut(s) 43, 338, 539
ApoI RAATTY 2 cut(s) 591, 615
AspS9I GGNCC 1 cut(s) 442
AsuC2I CCSGG 2 cut(s) 510, 581
AsuHPI GGTGA 3 cut(s) 110, 519, 591
BaeGI GKGCMC 1 cut(s) 192
BanII GRGCYC 1 cut(s) 213
Bbv12I GWGCWC 1 cut(s) 213
BbvCI CCTCAGC 1 cut(s) 259
BbvI GCAGC 3 cut(s) 55, 350, 551
BccI CCATC 1 cut(s) 628
BceAI ACGGC 1 cut(s) 97
BciT130I CCWGG 1 cut(s) 123
BclI TGATCA 1 cut(s) 115
BcnI CCSGG 2 cut(s) 510, 581
BcoDI GTCTC 2 cut(s) 505, 569
BfmI CTRYAG 3 cut(s) 336, 537, 557
BglII AGATCT 1 cut(s) 412
BisI GCNGC 5 cut(s) 44, 339, 399, 441, 540
BlsI GCNGC 5 cut(s) 45, 340, 400, 442, 541
Bme1390I CCNGG 3 cut(s) 123, 510, 581
BmgT120I GGNCC 1 cut(s) 442
BmiI GGNNCC 3 cut(s) 444, 585, 606
BmrFI CCNGG 3 cut(s) 123, 510, 581
BmrI ACTGGG 2 cut(s) 334, 567
BmsI GCATC 3 cut(s) 151, 172, 360
BmuI ACTGGG 2 cut(s) 334, 567
Bpu10I CCTNAGC 1 cut(s) 259
BpuEI CTTGAG 1 cut(s) 290
BpuMI CCSGG 2 cut(s) 510, 581
BsaJI CCNNGG 1 cut(s) 121
BsaWI WCCGGW 4 cut(s) 484, 586, 601, 634
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 5 cut(s) 127, 457, 484, 586, 634
Bse118I RCCGGY 5 cut(s) 436, 457, 461, 477, 607
Bse1I ACTGG 4 cut(s) 157, 289, 329, 573
BseBI CCWGG 1 cut(s) 123
BseDI CCNNGG 1 cut(s) 121
BseGI GGATG 2 cut(s) 118, 136
BseLI CCNNNNNNNGG 5 cut(s) 127, 457, 484, 586, 634
BseMII CTCAG 1 cut(s) 273
BseNI ACTGG 4 cut(s) 157, 289, 329, 573
BseSI GKGCMC 1 cut(s) 192
BseX3I CGGCCG 1 cut(s) 459
BseXI GCAGC 3 cut(s) 55, 350, 551
BseYI CCCAGC 1 cut(s) 185
Bsh1285I CGRYCG 1 cut(s) 462
BshFI GGCC 4 cut(s) 443, 461, 481, 611
BsiEI CGRYCG 1 cut(s) 462
BsiHKAI GWGCWC 1 cut(s) 213
BslI CCNNNNNNNGG 5 cut(s) 127, 457, 484, 586, 634
BsmAI GTCTC 2 cut(s) 505, 569
BsmBI CGTCTC 1 cut(s) 569
BsnI GGCC 4 cut(s) 443, 461, 481, 611
Bsp1286I GDGCHC 2 cut(s) 192, 213
Bsp143I GATC 3 cut(s) 69, 115, 412
BspACI CCGC 6 cut(s) 255, 293, 396, 399, 440, 629
BspANI GGCC 4 cut(s) 443, 461, 481, 611
BspCNI CTCAG 1 cut(s) 272
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 3 cut(s) 444, 585, 606
BspMAI CTGCAG 2 cut(s) 340, 541
BspPI GGATC 1 cut(s) 77
BsrFI RCCGGY 5 cut(s) 436, 457, 461, 477, 607
BsrI ACTGG 4 cut(s) 157, 289, 329, 573
BssAI RCCGGY 5 cut(s) 436, 457, 461, 477, 607
BssECI CCNNGG 1 cut(s) 121
BssMI GATC 3 cut(s) 69, 115, 412
Bst2UI CCWGG 1 cut(s) 123
BstC8I GCNNGC 1 cut(s) 463
BstDEI CTNAG 1 cut(s) 259
BstF5I GGATG 2 cut(s) 118, 136
BstKTI GATC 3 cut(s) 72, 118, 415
BstMAI GTCTC 2 cut(s) 505, 569
BstMBI GATC 3 cut(s) 69, 115, 412
BstMCI CGRYCG 1 cut(s) 462
BstMWI GCNNNNNNNGC 2 cut(s) 16, 335
BstNI CCWGG 1 cut(s) 123
BstSCI CCNGG 3 cut(s) 121, 508, 579
BstSFI CTRYAG 3 cut(s) 336, 537, 557
BstSLI GKGCMC 1 cut(s) 192
BstV1I GCAGC 3 cut(s) 55, 350, 551
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BstZI CGGCCG 1 cut(s) 459
BsuRI GGCC 4 cut(s) 443, 461, 481, 611
BtsCI GGATG 2 cut(s) 118, 136
BtsIMutI CAGTG 1 cut(s) 165
Cac8I GCNNGC 1 cut(s) 463
CciI TCATGA 1 cut(s) 303
Cfr10I RCCGGY 5 cut(s) 436, 457, 461, 477, 607
Cfr13I GGNCC 1 cut(s) 442
CseI GACGC 2 cut(s) 16, 551
Csp6I GTAC 1 cut(s) 91
CviAII CATG 3 cut(s) 214, 223, 304
CviQI GTAC 1 cut(s) 91
DdeI CTNAG 1 cut(s) 259
DpnI GATC 3 cut(s) 71, 117, 414
DpnII GATC 3 cut(s) 69, 115, 412
EaeI YGGCCR 3 cut(s) 459, 479, 609
EagI CGGCCG 1 cut(s) 459
EciI GGCGGA 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 211
EclXI CGGCCG 1 cut(s) 459
Eco24I GRGCYC 1 cut(s) 213
Eco52I CGGCCG 1 cut(s) 459
Eco53kI GAGCTC 1 cut(s) 211
EcoICRI GAGCTC 1 cut(s) 211
EcoRII CCWGG 1 cut(s) 121
EcoT38I GRGCYC 1 cut(s) 213
Esp3I CGTCTC 1 cut(s) 569
FaeI CATG 3 cut(s) 217, 226, 307
FaiI YATR 5 cut(s) 106, 215, 224, 305, 545
FatI CATG 3 cut(s) 213, 222, 303
FauI CCCGC 1 cut(s) 636
FbaI TGATCA 1 cut(s) 115
FblI GTMKAC 1 cut(s) 78
Fnu4HI GCNGC 5 cut(s) 44, 339, 399, 441, 540
FokI GGATG 2 cut(s) 125, 143
FriOI GRGCYC 1 cut(s) 213
Fsp4HI GCNGC 5 cut(s) 44, 339, 399, 441, 540
GluI GCNGC 5 cut(s) 44, 339, 399, 441, 540
GsaI CCCAGC 1 cut(s) 189
HaeIII GGCC 4 cut(s) 443, 461, 481, 611
HgaI GACGC 2 cut(s) 16, 551
Hin1II CATG 3 cut(s) 217, 226, 307
HincII GTYRAC 2 cut(s) 79, 102
HindII GTYRAC 2 cut(s) 79, 102
HinfI GANTC 3 cut(s) 274, 427, 638
HphI GGTGA 3 cut(s) 110, 519, 591
Hpy166II GTNNAC 3 cut(s) 79, 91, 102
Hpy188III TCNNGA 2 cut(s) 304, 431
Hpy8I GTNNAC 3 cut(s) 79, 91, 102
Hpy99I CGWCG 4 cut(s) 10, 77, 80, 83
HpyAV CCTTC 2 cut(s) 386, 531
HpyCH4V TGCA 5 cut(s) 31, 203, 228, 338, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 16, 335
HpyF3I CTNAG 1 cut(s) 259
Hsp92II CATG 3 cut(s) 217, 226, 307
KroI GCCGGC 1 cut(s) 461
KroNI GCCGGC 1 cut(s) 463
Ksp22I TGATCA 1 cut(s) 115
Kzo9I GATC 3 cut(s) 69, 115, 412
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 3 cut(s) 55, 350, 551
LweI GCATC 3 cut(s) 151, 172, 360
MaeIII GTNAC 1 cut(s) 575
MalI GATC 3 cut(s) 71, 117, 414
MboI GATC 3 cut(s) 69, 115, 412
MboII GAAGA 2 cut(s) 191, 422
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 192, 213
MluCI AATT 2 cut(s) 591, 615
MlyI GAGTC 1 cut(s) 436
MnlI CCTC 5 cut(s) 49, 262, 268, 535, 543
MroNI GCCGGC 1 cut(s) 461
MseI TTAA 1 cut(s) 646
MslI CAYNNNNRTG 2 cut(s) 123, 356
MspA1I CMGCKG 2 cut(s) 263, 341
MspR9I CCNGG 3 cut(s) 123, 510, 581
MvaI CCWGG 1 cut(s) 123
MwoI GCNNNNNNNGC 2 cut(s) 16, 335
NaeI GCCGGC 1 cut(s) 463
NciI CCSGG 2 cut(s) 510, 581
NdeII GATC 3 cut(s) 69, 115, 412
NgoMIV GCCGGC 1 cut(s) 461
NlaIII CATG 3 cut(s) 217, 226, 307
NlaIV GGNNCC 3 cut(s) 444, 585, 606
OliI CACNNNNGTG 1 cut(s) 123
PagI TCATGA 1 cut(s) 303
PdiI GCCGGC 1 cut(s) 463
PfeI GAWTC 2 cut(s) 274, 638
PkrI GCNGC 5 cut(s) 45, 340, 400, 442, 541
PleI GAGTC 1 cut(s) 435
PpsI GAGTC 1 cut(s) 435
Psp124BI GAGCTC 1 cut(s) 213
Psp6I CCWGG 1 cut(s) 121
PspFI CCCAGC 1 cut(s) 185
PspGI CCWGG 1 cut(s) 121
PspN4I GGNNCC 3 cut(s) 444, 585, 606
PspPI GGNCC 1 cut(s) 442
PstI CTGCAG 2 cut(s) 340, 541
PsuI RGATCY 1 cut(s) 412
PvuII CAGCTG 2 cut(s) 263, 341
RsaI GTAC 1 cut(s) 92
RsaNI GTAC 1 cut(s) 91
RseI CAYNNNNRTG 2 cut(s) 123, 356
SacI GAGCTC 1 cut(s) 213
SalI GTCGAC 1 cut(s) 77
SaqAI TTAA 1 cut(s) 646
SatI GCNGC 5 cut(s) 44, 339, 399, 441, 540
Sau3AI GATC 3 cut(s) 69, 115, 412
Sau96I GGNCC 1 cut(s) 442
SchI GAGTC 1 cut(s) 436
ScrFI CCNGG 3 cut(s) 123, 510, 581
SduI GDGCHC 2 cut(s) 192, 213
SfaNI GCATC 3 cut(s) 151, 172, 360
SfcI CTRYAG 3 cut(s) 336, 537, 557
SgrDI CGTCGACG 1 cut(s) 77
SmiMI CAYNNNNRTG 2 cut(s) 123, 356
SmlI CTYRAG 1 cut(s) 269
SmoI CTYRAG 1 cut(s) 269
Sse9I AATT 2 cut(s) 591, 615
SsiI CCGC 6 cut(s) 255, 293, 396, 399, 440, 629
SstI GAGCTC 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 121, 508, 579
TaqI TCGA 3 cut(s) 78, 549, 623
TasI AATT 2 cut(s) 591, 615
TauI GCSGC 2 cut(s) 401, 443
TfiI GAWTC 2 cut(s) 274, 638
Tru1I TTAA 1 cut(s) 646
Tru9I TTAA 1 cut(s) 646
TscAI CASTG 1 cut(s) 172
TseI GCWGC 3 cut(s) 43, 338, 539
TspDTI ATGAA 1 cut(s) 585
TspRI CASTG 1 cut(s) 172
XapI RAATTY 2 cut(s) 591, 615
XmiI GTMKAC 1 cut(s) 78
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.