Rmu_sc0035221.1_g000001
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0035221.1
OG0020027
Physical Location & Seq
Forward (+)
1 .. 517
517 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0035221.1_g000001.1.cds

Sequence Viewer

Length: 517 bp
tcggatcccgcccgagctcggaaacttaacgaacctcgaggtcctctggctgaccgagtgtaacttagtcggcgaaataccggactcgctgggtcgactcagtaaactcaccgatttggacctcgccattaacgccctgcacggctcgattccggcgtcgttgaccgagttgaccagcgtggttcagattgagctctataacaacaccttgaccggaggcctcccgccgggaatgtcgaagctgaagaatctccggctgctcgacgcgtccatgaaccggctgacggggccgattcccgacgagttgactcagctggagctggaaagcctgaatctttacgagaacctcttcgaggggagtttgccagcgagcattgccgactcgccgaacctgtacgagctccggctgtttcagaacaagctcaccggcgagctaccgcggaatctcggccagaactcgccgctgaggtggatagacctgtcgaacaaccagttttccgggagtattccggcgagt
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

172

Amino Acids

18.83

Weight (kDa)

4.5

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0020027)

Species Orthologous Gene IDs
rosa_laevigata RLG00000016946
rosa_multiflora Rmu_sc0000157.1_g000040 Rmu_sc0002819.1_g000008 Rmu_sc0004199.1_g000024 Rmu_sc0035221.1_g000001
rosa_roxburghii Rroxscaffold_2G00140740
rosa_samantha Rh5CG217800

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 36
AccI GTMKAC 1 cut(s) 95
AccII CGCG 2 cut(s) 267, 440
AciI CCGC 5 cut(s) 9, 225, 438, 440, 462
AclWI GGATC 1 cut(s) 12
AcoI YGGCCR 1 cut(s) 449
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 156
AfaI GTAC 1 cut(s) 396
AfiI CCNNNNNNNGG 5 cut(s) 18, 227, 277, 284, 353
AflIII ACRYGT 1 cut(s) 265
AluBI AGCT 8 cut(s) 17, 194, 242, 314, 320, 401, 422, 434
AluI AGCT 8 cut(s) 17, 194, 242, 314, 320, 401, 422, 434
Alw21I GWGCWC 3 cut(s) 19, 196, 403
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 2 cut(s) 12, 36
AoxI GGCC 3 cut(s) 218, 288, 449
ApeKI GCWGC 1 cut(s) 257
Asp700I GAANNNNTTC 1 cut(s) 348
AspS9I GGNCC 3 cut(s) 41, 119, 288
AsuC2I CCSGG 2 cut(s) 229, 500
AsuHPI GGTGA 2 cut(s) 101, 416
AvaI CYCGRG 2 cut(s) 12, 36
AvaII GGWCC 2 cut(s) 41, 119
BamHI GGATCC 1 cut(s) 4
BanII GRGCYC 3 cut(s) 19, 196, 403
Bbv12I GWGCWC 3 cut(s) 19, 196, 403
BbvCI CCTCAGC 1 cut(s) 465
BbvI GCAGC 1 cut(s) 244
BceAI ACGGC 1 cut(s) 158
BcnI CCSGG 2 cut(s) 229, 500
BisI GCNGC 2 cut(s) 258, 462
BlsI GCNGC 2 cut(s) 259, 463
Bme1390I CCNGG 2 cut(s) 229, 500
Bme18I GGWCC 2 cut(s) 41, 119
BmeT110I CYCGRG 2 cut(s) 12, 36
BmgT120I GGNCC 3 cut(s) 41, 119, 288
BmiI GGNNCC 2 cut(s) 6, 289
BmrFI CCNGG 2 cut(s) 229, 500
BpmI CTGGAG 1 cut(s) 336
Bpu10I CCTNAGC 1 cut(s) 465
BpuMI CCSGG 2 cut(s) 229, 500
BsaHI GRCGYC 1 cut(s) 156
BsaJI CCNNGG 1 cut(s) 438
BsaWI WCCGGW 2 cut(s) 80, 213
Bsc4I CCNNNNNNNGG 5 cut(s) 18, 227, 277, 284, 353
Bse118I RCCGGY 2 cut(s) 277, 426
Bse1I ACTGG 1 cut(s) 491
Bse3DI GCAATG 1 cut(s) 373
BseDI CCNNGG 1 cut(s) 438
BseLI CCNNNNNNNGG 5 cut(s) 18, 227, 277, 284, 353
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 3 cut(s) 113, 324, 456
BseNI ACTGG 1 cut(s) 491
BseXI GCAGC 1 cut(s) 244
BseYI CCCAGC 1 cut(s) 89
BsgI GTGCAG 1 cut(s) 123
Bsh1236I CGCG 2 cut(s) 267, 440
BshFI GGCC 3 cut(s) 220, 290, 451
BsiHKAI GWGCWC 3 cut(s) 19, 196, 403
BsiHKCI CYCGRG 2 cut(s) 12, 36
BslI CCNNNNNNNGG 5 cut(s) 18, 227, 277, 284, 353
BsnI GGCC 3 cut(s) 220, 290, 451
BsoBI CYCGRG 2 cut(s) 12, 36
Bsp1286I GDGCHC 3 cut(s) 19, 196, 403
Bsp143I GATC 1 cut(s) 4
BspACI CCGC 5 cut(s) 9, 225, 438, 440, 462
BspANI GGCC 3 cut(s) 220, 290, 451
BspCNI CTCAG 3 cut(s) 112, 323, 457
BspFNI CGCG 2 cut(s) 267, 440
BspLI GGNNCC 2 cut(s) 6, 289
BspPI GGATC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 373
BsrFI RCCGGY 2 cut(s) 277, 426
BsrI ACTGG 1 cut(s) 491
BssAI RCCGGY 2 cut(s) 277, 426
BssECI CCNNGG 1 cut(s) 438
BssMI GATC 1 cut(s) 4
BssNI GRCGYC 1 cut(s) 156
Bst6I CTCTTC 1 cut(s) 354
BstACI GRCGYC 1 cut(s) 156
BstC8I GCNNGC 3 cut(s) 367, 371, 432
BstDEI CTNAG 4 cut(s) 65, 99, 310, 465
BstDSI CCRYGG 1 cut(s) 438
BstENI CCTNNNNNAGG 1 cut(s) 351
BstFNI CGCG 2 cut(s) 267, 440
BstKTI GATC 1 cut(s) 7
BstMBI GATC 1 cut(s) 4
BstMWI GCNNNNNNNGC 3 cut(s) 132, 287, 375
BstSCI CCNGG 2 cut(s) 227, 498
BstUI CGCG 2 cut(s) 267, 440
BstV1I GCAGC 1 cut(s) 244
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BsuRI GGCC 3 cut(s) 220, 290, 451
BtgI CCRYGG 1 cut(s) 438
Cac8I GCNNGC 3 cut(s) 367, 371, 432
Cfr10I RCCGGY 2 cut(s) 277, 426
Cfr13I GGNCC 3 cut(s) 41, 119, 288
Cfr42I CCGCGG 1 cut(s) 441
CseI GACGC 3 cut(s) 145, 256, 273
Csp6I GTAC 1 cut(s) 395
CviAII CATG 1 cut(s) 272
CviQI GTAC 1 cut(s) 395
DdeI CTNAG 4 cut(s) 65, 99, 310, 465
DpnI GATC 1 cut(s) 6
DpnII GATC 1 cut(s) 4
EaeI YGGCCR 1 cut(s) 449
Eam1104I CTCTTC 1 cut(s) 354
EarI CTCTTC 1 cut(s) 354
Ecl136II GAGCTC 3 cut(s) 17, 194, 401
Eco147I AGGCCT 1 cut(s) 220
Eco24I GRGCYC 3 cut(s) 19, 196, 403
Eco47I GGWCC 2 cut(s) 41, 119
Eco53kI GAGCTC 3 cut(s) 17, 194, 401
Eco57I CTGAAG 1 cut(s) 264
Eco88I CYCGRG 2 cut(s) 12, 36
EcoICRI GAGCTC 3 cut(s) 17, 194, 401
EcoNI CCTNNNNNAGG 1 cut(s) 351
EcoO109I RGGNCCY 1 cut(s) 41
EcoT38I GRGCYC 3 cut(s) 19, 196, 403
FaeI CATG 1 cut(s) 275
FaiI YATR 2 cut(s) 199, 273
FatI CATG 1 cut(s) 271
FauI CCCGC 2 cut(s) 16, 232
FblI GTMKAC 1 cut(s) 95
Fnu4HI GCNGC 2 cut(s) 258, 462
FriOI GRGCYC 3 cut(s) 19, 196, 403
Fsp4HI GCNGC 2 cut(s) 258, 462
GluI GCNGC 2 cut(s) 258, 462
GsaI CCCAGC 1 cut(s) 93
GsuI CTGGAG 1 cut(s) 336
HaeIII GGCC 3 cut(s) 220, 290, 451
HgaI GACGC 3 cut(s) 145, 256, 273
Hin1I GRCGYC 1 cut(s) 156
Hin1II CATG 1 cut(s) 275
HincII GTYRAC 4 cut(s) 96, 163, 172, 307
HindII GTYRAC 4 cut(s) 96, 163, 172, 307
HinfI GANTC 9 cut(s) 84, 97, 149, 248, 293, 308, 332, 381, 443
HphI GGTGA 2 cut(s) 101, 416
Hpy166II GTNNAC 5 cut(s) 96, 105, 163, 172, 307
Hpy188I TCNGA 4 cut(s) 4, 21, 187, 415
Hpy188III TCNNGA 1 cut(s) 297
Hpy8I GTNNAC 5 cut(s) 96, 105, 163, 172, 307
Hpy99I CGWCG 3 cut(s) 161, 267, 303
HpyCH4V TGCA 1 cut(s) 140
HpyF10VI GCNNNNNNNGC 3 cut(s) 132, 287, 375
HpyF3I CTNAG 4 cut(s) 65, 99, 310, 465
Hsp92I GRCGYC 1 cut(s) 156
Hsp92II CATG 1 cut(s) 275
KspI CCGCGG 1 cut(s) 441
Kzo9I GATC 1 cut(s) 4
LmnI GCTCC 2 cut(s) 317, 406
Lsp1109I GCAGC 1 cut(s) 244
MaeIII GTNAC 1 cut(s) 60
MalI GATC 1 cut(s) 6
MboI GATC 1 cut(s) 4
MboII GAAGA 2 cut(s) 257, 341
MflI RGATCY 1 cut(s) 4
MhlI GDGCHC 3 cut(s) 19, 196, 403
MluI ACGCGT 1 cut(s) 265
MlyI GAGTC 4 cut(s) 78, 91, 302, 375
MnlI CCTC 9 cut(s) 32, 45, 54, 132, 210, 231, 347, 357, 460
MroXI GAANNNNTTC 1 cut(s) 348
MseI TTAA 2 cut(s) 27, 130
MspA1I CMGCKG 3 cut(s) 314, 440, 464
MspR9I CCNGG 2 cut(s) 229, 500
MvnI CGCG 2 cut(s) 267, 440
MwoI GCNNNNNNNGC 3 cut(s) 132, 287, 375
NciI CCSGG 2 cut(s) 229, 500
NdeII GATC 1 cut(s) 4
NlaIII CATG 1 cut(s) 275
NlaIV GGNNCC 2 cut(s) 6, 289
NmeAIII GCCGAG 1 cut(s) 427
PaeR7I CTCGAG 1 cut(s) 36
PceI AGGCCT 1 cut(s) 220
PcsI WCGNNNNNNNCGW 1 cut(s) 153
PdmI GAANNNNTTC 1 cut(s) 348
PfeI GAWTC 5 cut(s) 149, 248, 293, 332, 443
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 2 cut(s) 259, 463
PleI GAGTC 4 cut(s) 78, 91, 302, 375
PpsI GAGTC 4 cut(s) 78, 91, 302, 375
PpuMI RGGWCCY 1 cut(s) 41
Psp124BI GAGCTC 3 cut(s) 19, 196, 403
Psp5II RGGWCCY 1 cut(s) 41
PspFI CCCAGC 1 cut(s) 89
PspN4I GGNNCC 2 cut(s) 6, 289
PspPI GGNCC 3 cut(s) 41, 119, 288
PspPPI RGGWCCY 1 cut(s) 41
PspXI VCTCGAGB 1 cut(s) 36
PsuI RGATCY 1 cut(s) 4
PvuII CAGCTG 1 cut(s) 314
RsaI GTAC 1 cut(s) 396
RsaNI GTAC 1 cut(s) 395
SacI GAGCTC 3 cut(s) 19, 196, 403
SacII CCGCGG 1 cut(s) 441
SalI GTCGAC 1 cut(s) 94
SaqAI TTAA 2 cut(s) 27, 130
SatI GCNGC 2 cut(s) 258, 462
Sau3AI GATC 1 cut(s) 4
Sau96I GGNCC 3 cut(s) 41, 119, 288
SchI GAGTC 4 cut(s) 78, 91, 302, 375
ScrFI CCNGG 2 cut(s) 229, 500
SduI GDGCHC 3 cut(s) 19, 196, 403
Sfr274I CTCGAG 1 cut(s) 36
Sfr303I CCGCGG 1 cut(s) 441
SgrAI CRCCGGYG 1 cut(s) 426
SgrBI CCGCGG 1 cut(s) 441
SinI GGWCC 2 cut(s) 41, 119
SlaI CTCGAG 1 cut(s) 36
SmlI CTYRAG 1 cut(s) 36
SmoI CTYRAG 1 cut(s) 36
SseBI AGGCCT 1 cut(s) 220
SsiI CCGC 5 cut(s) 9, 225, 438, 440, 462
SstI GAGCTC 3 cut(s) 19, 196, 403
StuI AGGCCT 1 cut(s) 220
StyD4I CCNGG 2 cut(s) 227, 498
TaqI TCGA 7 cut(s) 37, 95, 147, 237, 262, 352, 483
TaqII GACCGA 2 cut(s) 69, 180
TauI GCSGC 1 cut(s) 464
TfiI GAWTC 5 cut(s) 149, 248, 293, 332, 443
Tru1I TTAA 2 cut(s) 27, 130
Tru9I TTAA 2 cut(s) 27, 130
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 288
VpaK11BI GGWCC 2 cut(s) 41, 119
XagI CCTNNNNNAGG 1 cut(s) 351
XhoI CTCGAG 1 cut(s) 36
XmiI GTMKAC 1 cut(s) 95
XmnI GAANNNNTTC 1 cut(s) 348
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.